CA2223155A1 - Secretion leader trap cloning method - Google Patents

Secretion leader trap cloning method

Info

Publication number
CA2223155A1
CA2223155A1 CA002223155A CA2223155A CA2223155A1 CA 2223155 A1 CA2223155 A1 CA 2223155A1 CA 002223155 A CA002223155 A CA 002223155A CA 2223155 A CA2223155 A CA 2223155A CA 2223155 A1 CA2223155 A1 CA 2223155A1
Authority
CA
Canada
Prior art keywords
orphan
methods
cloning method
secretion leader
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002223155A
Other languages
French (fr)
Other versions
CA2223155C (en
Inventor
Si Lok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2223155A1 publication Critical patent/CA2223155A1/en
Application granted granted Critical
Publication of CA2223155C publication Critical patent/CA2223155C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Expression vectors and methods for cloning amino-terminal signal sequences are disclosed. These vectors and methods are useful tools for selecting, expressing and isolating previously unknown signal sequences. In turn, an orphan signal sequence can be used to clone, identify and isolate the corresponding orphan proteins that the orphan signal sequence directs to the surface of a cell. Using these expression vectors and methods, an orphan protein can be cloned in the absence of a functional assay that detects such protein.
CA002223155A 1995-06-07 1996-06-04 Secretion leader trap cloning method Expired - Fee Related CA2223155C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US48349395A 1995-06-07 1995-06-07
US47280695A 1995-06-07 1995-06-07
US08/472,806 1995-06-07
US08/483,493 1995-06-07
PCT/US1996/008936 WO1996040904A1 (en) 1995-06-07 1996-06-04 Secretion leader trap cloning method

Publications (2)

Publication Number Publication Date
CA2223155A1 true CA2223155A1 (en) 1996-12-19
CA2223155C CA2223155C (en) 2003-08-12

Family

ID=27043933

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002223155A Expired - Fee Related CA2223155C (en) 1995-06-07 1996-06-04 Secretion leader trap cloning method

Country Status (5)

Country Link
EP (1) EP0832209A1 (en)
JP (1) JPH11506939A (en)
AU (1) AU6045196A (en)
CA (1) CA2223155C (en)
WO (1) WO1996040904A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
GB9701492D0 (en) * 1997-01-24 1997-03-12 Univ Edinburgh Transfection of embryonic stem cells
WO1999005256A2 (en) * 1997-07-24 1999-02-04 President And Fellows Of Harvard College Method for cloning secreted proteins
RU2230751C2 (en) 1997-11-26 2004-06-20 Чугаи Сейяку Кабусики Кайся Peptide mpi without signal sequence, dna encoding thereof, vector, method for assay capacity for secretion of testing peptide, method for isolating cdna
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
US6103472A (en) * 1998-02-20 2000-08-15 Amgen Inc. Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
US6228590B1 (en) 1998-03-23 2001-05-08 Genentech, Inc. Method of selection for genes encoding secreted and transmembrane proteins
DE69931776T2 (en) 1998-05-20 2007-05-16 Chugai Seiyaku K.K. METHOD OF CLONING GENES
WO2001009310A1 (en) * 1999-07-29 2001-02-08 Berlex Laboratories, Inc. 5'ENRICHED cDNA LIBRARIES AND A YEAST SIGNAL TRAP
US7112434B2 (en) 2001-05-02 2006-09-26 University Of South Florida Vector system for selection of genes encoding secreted proteins and membrane-bound proteins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE110111T1 (en) * 1986-05-02 1994-09-15 Gist Brocades Nv SECRETION SIGNAL SELECTION VECTORS FOR EXTRACELLULAR PROTEIN SYNTHESIS IN BACILLA.
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
DE3901681A1 (en) * 1989-01-21 1990-07-26 Behringwerke Ag SIGNAL PEPTIDE FOR THE SECRETION OF PEPTIDES IN ESCHERICHIA COLI, METHOD FOR ITS EFFECT AND ITS USE
GB8926269D0 (en) * 1989-11-21 1990-01-10 Dynal As Plasmid
JP2879303B2 (en) * 1993-01-14 1999-04-05 佑 本庶 Method for preparing cDNA library, novel polypeptide and DNA encoding the same
DE4332463A1 (en) * 1993-09-24 1995-03-30 Boehringer Mannheim Gmbh Process for the specific cloning of nucleic acids

Also Published As

Publication number Publication date
EP0832209A1 (en) 1998-04-01
CA2223155C (en) 2003-08-12
JPH11506939A (en) 1999-06-22
AU6045196A (en) 1996-12-30
WO1996040904A1 (en) 1996-12-19

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