MX428A - Region regulatoria para expresion de gen heterologa en levadura. - Google Patents

Region regulatoria para expresion de gen heterologa en levadura.

Info

Publication number
MX428A
MX428A MX428A MX42885A MX428A MX 428 A MX428 A MX 428A MX 428 A MX428 A MX 428A MX 42885 A MX42885 A MX 42885A MX 428 A MX428 A MX 428A
Authority
MX
Mexico
Prior art keywords
relates
expression
yeast
regulatory region
dna
Prior art date
Application number
MX428A
Other languages
English (en)
Inventor
David Womack Stroman
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Publication of MX428A publication Critical patent/MX428A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/31—Linker sequence

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención se refiere al campo de biotecnología de ADN recombinante. En uno de sus aspectos, la invencíon se refiere a fragmentos de ADN que regulan la transcripción de ADN en ARN mensajero, y la iniciación y término de la traducción de ARN mensajero en proteína. en otro aspecto, la invención se refiere a vectores de expresión que incorporan los fragmentos de ADN anteriormente descritos. Aún en otro aspecto, la invención se refiere a microorganismos novedosos transformados con los vectores de expresión anteriormente descritos. En un aspecto adicional, la invención se refiere a la producción de polipéptidos.
MX428A 1984-10-30 1985-10-29 Region regulatoria para expresion de gen heterologa en levadura. MX428A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use

Publications (1)

Publication Number Publication Date
MX428A true MX428A (es) 1994-03-31

Family

ID=24673982

Family Applications (1)

Application Number Title Priority Date Filing Date
MX428A MX428A (es) 1984-10-30 1985-10-29 Region regulatoria para expresion de gen heterologa en levadura.

Country Status (3)

Country Link
US (1) US4808537A (es)
MX (1) MX428A (es)
ZA (1) ZA858181B (es)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US5240838A (en) * 1984-07-27 1993-08-31 Internationale Otrool Maatschappij "Octropa" B.V. Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha
US5316931A (en) * 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US6054566A (en) * 1988-02-26 2000-04-25 Biosource Technologies, Inc. Recombinant animal viral nucleic acids
US20030150019A1 (en) * 1988-02-26 2003-08-07 Large Scale Biology Corporation Monopartite RNA virus transformation vectors
US6284492B1 (en) 1988-02-26 2001-09-04 Large Scale Biology Corporation Recombinant animal viral nucleic acids
WO1990001068A1 (en) * 1988-07-22 1990-02-08 Life Technologies, Inc. Sequence specific assay for the detection of a nucleic acid molecule
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
KR100342948B1 (ko) * 1993-03-03 2002-11-29 유니버시티 오브 아이오와 리써치 파운데이션 메틸로트로픽효모에서글리콜레이트산화효소의제조방법
US5500483A (en) * 1993-03-25 1996-03-19 Biosource Technologies, Inc. Pichia pastoris alcohol oxidase ZZA1 regulatory region for heterologous gene expression
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
HU225126B1 (en) * 1994-10-18 2006-06-28 Dendreon Corp Nematode-extracted serine protease inhibitors and anticoagulant proteins
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5955349A (en) * 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5716808A (en) * 1995-11-09 1998-02-10 Zymogenetics, Inc. Genetic engineering of pichia methanolica
CA2237039A1 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Production of gad65 in methylotrophic yeast
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
NZ504214A (en) * 1997-10-24 2003-06-30 Invitrogen Corp Recombination cloning using nucleic acids having recombination sites
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US6730499B1 (en) * 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
EP1114148A4 (en) * 1998-08-28 2004-12-22 Invitrogen Corp SYSTEM FOR RAPID HANDLING OF NUCLEIC ACID SEQUENCES
EP1173460B1 (en) 1999-03-02 2009-09-16 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
WO2001042509A1 (en) 1999-12-10 2001-06-14 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1385866A4 (en) * 2001-04-05 2006-03-29 Univ Nebraska ALCOHOL OXIDASE 1 REGULATORY NUCLEOTIDE SEQUENCES FOR THE EXPRESSION OF HETEROLOGOUS GENES IN YEAST
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
AU2003253992A1 (en) * 2002-07-18 2004-02-09 Robert P. Bennett Viral vectors containing recombination sites
DE10248258A1 (de) * 2002-10-16 2004-05-06 Biopsytec Analytik Gmbh Mutierte Nukleinsäure einer Cel I-Endonuklease und Verfahren zur Herstellung des rekombinanten vollständigen Cel I-Proteins
US7118901B2 (en) * 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
US8304189B2 (en) * 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
ATE455849T1 (de) * 2003-12-05 2010-02-15 Hoffmann La Roche Rekombinante carboxypeptidase b und ihre aufreinigung
KR101304958B1 (ko) * 2005-09-14 2013-09-17 에프. 호프만-라 로슈 아게 트립신 변이체에 의한 인슐린 전구체의 절단
KR20100120708A (ko) 2008-03-03 2010-11-16 아보트 러보러터리즈 효모를 형질전환시키는 방법
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
MX2013007621A (es) 2011-01-18 2013-10-03 Syngenta Participations Ag Metodos y composiciones para sistemas de promotores inducibles por etanol modificados.
IN2014DN03419A (es) 2011-10-07 2015-05-22 Lonza Ag
ES2751336T3 (es) 2013-03-15 2020-03-31 Lonza Ag Promotor constitutivo
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter

Also Published As

Publication number Publication date
ZA858181B (en) 1986-06-25
US4808537A (en) 1989-02-28

Similar Documents

Publication Publication Date Title
MX428A (es) Region regulatoria para expresion de gen heterologa en levadura.
BG60107B2 (bg) Метод за получаване на еукариотен полипептид
BR8301918A (pt) Proteina,sequencia de dna,veiculo de expressao replicavel,microorganismo ou cultura celular,plasmideo,composicao,cultura de celulas,utilizacao de tal proteina,processo de expressao,processo para producao de um veiculo de expressao e processo para preparacao de uma proteina uroquinase
ES2056799T3 (es) Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
DK320686D0 (da) Ekspressionskontrolsekvenser
MX9304027A (es) Variante de al enzima lipasa y metodo para su produccion.
ES2013324A6 (es) Un metodo mejorado para producir una proteina heterologa.
DE3588255D1 (de) Klonierung und Expression von HTLV-III-DNS
EP0260148A3 (en) Improved recombinant expression method, vector and transformed cells
HUT46061A (en) Dna sequences encoding hirudin-like protein and process for producing such proteins
ES8703930A1 (es) Procedimiento de preparar hirudina y analogos a partir de levadura
IL88151A (en) Interleukin-7 protein, dna sequences encoding it, its preparation and compositions containing it
AU8110487A (en) DNA molecules coding for proteins targeted to plant cell walls
ES2076926T3 (es) Produccion por tecnologia genetica de factor xiiia.
ATE67242T1 (de) Verfahren zur verbesserung der expression von eukaryontenprotein.
DE3850774D1 (de) Rinder-interleukin-1-alpha.
ES2065915T3 (es) Procedimiento para aumentar la expresion de polipeptidos en un cultivo de celula recombinante.
EE03074B1 (et) Rekombinantsed eukarüootsed rakud, nende saamiseks vajalikud DNA järjestused, meetod eukarüootsete rakkude konstrueerimiseks ja sekreteeritavate valkude ning biomassi tootmismeetod
GR3020035T3 (en) Mammalian cell derived autonomously replicating sequence DNA fragments having affinity to DNA binding proteins
ATE113655T1 (de) Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten.
IT8521507A0 (it) Vettore di clonaggio, molecole di dna ricombinante, ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni.
DK577283D0 (da) Gen kodet for interleukin-2-polypeptid,rekombinant dna baerende dette gen,levende cellelinie indeholdende dette rekombinante dna og fremgangsmaade til fremstilling af interleukin-2 ved anvendelse af denne cellelinie
EP0183272A3 (en) Vector capable of efficient gene expression and utilization of such vector
EP0194626A3 (en) Method for transformation and direct selection of transformants in eucaryotic cells
ITMI931034A1 (it) Processo per la preparazione di proteine inibenti i ribosomi