ATE391789T1 - Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen - Google Patents
Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonenInfo
- Publication number
- ATE391789T1 ATE391789T1 AT00984184T AT00984184T ATE391789T1 AT E391789 T1 ATE391789 T1 AT E391789T1 AT 00984184 T AT00984184 T AT 00984184T AT 00984184 T AT00984184 T AT 00984184T AT E391789 T1 ATE391789 T1 AT E391789T1
- Authority
- AT
- Austria
- Prior art keywords
- molecules
- cloning
- compounds
- recombination
- recombinatory
- Prior art date
Links
- 238000005215 recombination Methods 0.000 title abstract 4
- 230000006798 recombination Effects 0.000 title abstract 4
- 238000010367 cloning Methods 0.000 title abstract 3
- 150000001875 compounds Chemical class 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 235000014633 carbohydrates Nutrition 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 150000002632 lipids Chemical class 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16998399P | 1999-12-10 | 1999-12-10 | |
US18802000P | 2000-03-09 | 2000-03-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE391789T1 true ATE391789T1 (de) | 2008-04-15 |
Family
ID=26865565
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00984184T ATE391789T1 (de) | 1999-12-10 | 2000-12-11 | Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen |
Country Status (12)
Country | Link |
---|---|
US (1) | US7393632B2 (de) |
EP (3) | EP1978098A3 (de) |
JP (1) | JP4729222B2 (de) |
CN (2) | CN100587068C (de) |
AT (1) | ATE391789T1 (de) |
AU (2) | AU785483B2 (de) |
CA (1) | CA2392753A1 (de) |
DE (1) | DE60038572T2 (de) |
DK (1) | DK1250453T3 (de) |
HK (1) | HK1062034A1 (de) |
NZ (3) | NZ530816A (de) |
WO (1) | WO2001042509A1 (de) |
Families Citing this family (119)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6964861B1 (en) * | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
US6720140B1 (en) * | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US7670823B1 (en) | 1999-03-02 | 2010-03-02 | Life Technologies Corp. | Compositions for use in recombinational cloning of nucleic acids |
US6274369B1 (en) * | 1996-02-02 | 2001-08-14 | Invitrogen Corporation | Method capable of increasing competency of bacterial cell transformation |
US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
CN101125873A (zh) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
DE69836092T2 (de) | 1997-10-24 | 2007-05-10 | Invitrogen Corp., Carlsbad | Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen |
JP2002502977A (ja) | 1998-02-04 | 2002-01-29 | インビトロジェン コーポレイション | マイクロアレイとその使用 |
AU766257C (en) | 1998-05-07 | 2004-06-03 | Universite Libre De Bruxelles | Cytotoxin-based biological containment |
US6991922B2 (en) | 1998-08-12 | 2006-01-31 | Proteus S.A. | Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation |
US6951719B1 (en) | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
JP4729222B2 (ja) | 1999-12-10 | 2011-07-20 | ライフ テクノロジーズ コーポレーション | 組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用 |
US7244560B2 (en) * | 2000-05-21 | 2007-07-17 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US7198924B2 (en) * | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
EP2105496B1 (de) * | 2000-12-08 | 2013-02-20 | Life Technologies Corporation | Verfahren und Zusammensetzungen zur Synthese von Nukleinsäuremolekülen unter Verwendung multipler Erkennungsstellen |
WO2002053576A1 (en) | 2001-01-05 | 2002-07-11 | The General Hospital Corporation | Viral delivery system for infectious transfer of large genomic dna inserts |
AU2002235676B2 (en) | 2001-02-23 | 2006-10-26 | Universite Libre De Bruxelles | Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to a toxic molecule |
WO2002079409A2 (en) * | 2001-03-29 | 2002-10-10 | Rutgers, The University Of New Jersey | Integrases for the insertion of heterologous nucleic acids into the plastid genome |
US20040132042A1 (en) * | 2001-04-06 | 2004-07-08 | Frankard Valerie Marie-Noelle | Use of double and opposite recombination sites or the single step cloning of two dna segments |
CA2444195A1 (en) * | 2001-04-19 | 2002-10-31 | Invitrogen Corporation | Compositions and methods for recombinational cloning of nucleic acid molecules |
CA2448505A1 (en) * | 2001-05-21 | 2002-11-28 | Invitrogen Corporation | Compositions and methods for use in isolation of nucleic acid molecules |
US20030059888A1 (en) * | 2001-08-22 | 2003-03-27 | Shengfeng Li | Compositions and methods for generating antigen-binding units |
US7985553B2 (en) | 2001-10-29 | 2011-07-26 | Nathaniel Heintz | Method for isolating cell type-specific mRNAs |
JP4210769B2 (ja) * | 2001-11-22 | 2009-01-21 | 独立行政法人産業技術総合研究所 | Gatewayエントリークローンの作製方法 |
US7056665B2 (en) * | 2002-01-16 | 2006-06-06 | Panomics, Inc. | Screening methods involving the detection of short-lived proteins |
US6955882B2 (en) * | 2002-01-23 | 2005-10-18 | Cropdesign N.V. | Method for easy cloning and selection of chimeric DNA molecules |
US6818420B2 (en) | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
US20040005710A1 (en) * | 2002-03-09 | 2004-01-08 | Ho-Sun Son | Method for producing recombinant viruses using site-specific recombination |
US7319001B2 (en) * | 2002-03-09 | 2008-01-15 | Neurogenex Co., Ltd. | High throughput system for producing recombinant viruses using site-specific recombination |
WO2003078638A1 (en) * | 2002-03-19 | 2003-09-25 | Universite Libre De Bruxelles | Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms |
US20040038304A1 (en) * | 2002-03-28 | 2004-02-26 | Gala Design, Inc. | Antibody libraries |
US20030228608A1 (en) * | 2002-04-16 | 2003-12-11 | Promega Corporation | Method to enhance homologous recombination |
US20040106566A1 (en) * | 2002-05-17 | 2004-06-03 | Shi-Lung Lin | RNA-splicing and processing-directed gene silencing and the relative applications thereof |
AU2003239897A1 (en) * | 2002-05-23 | 2003-12-12 | Ceptyr, Inc. | Modulation of ptp1b signal transduction by rna interference |
US7527966B2 (en) | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
US20040172667A1 (en) * | 2002-06-26 | 2004-09-02 | Cooper Richard K. | Administration of transposon-based vectors to reproductive organs |
US20040132133A1 (en) * | 2002-07-08 | 2004-07-08 | Invitrogen Corporation | Methods and compositions for the production, identification and purification of fusion proteins |
WO2004009768A2 (en) * | 2002-07-18 | 2004-01-29 | Invitrogen Corporation | Viral vectors containing recombination sites |
CA2496514C (en) | 2002-09-03 | 2012-11-06 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
US9309518B2 (en) | 2002-09-03 | 2016-04-12 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
WO2004024940A2 (en) * | 2002-09-16 | 2004-03-25 | University Of Southern California | Rna-mediated gene modulation |
CN1263860C (zh) * | 2002-09-30 | 2006-07-12 | 华南农业大学 | 多基因载体的构建方法与应用 |
WO2004029284A2 (en) * | 2002-09-30 | 2004-04-08 | Protein Design Labs, Inc. | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
US7267984B2 (en) * | 2002-10-31 | 2007-09-11 | Rice University | Recombination assembly of large DNA fragments |
US7629120B2 (en) | 2002-10-31 | 2009-12-08 | Rice University | Method for assembling PCR fragments of DNA |
TW200502390A (en) * | 2002-11-28 | 2005-01-16 | Peter Droege | Sequence specific DNA recombination in eukaryotic cells |
US7491539B2 (en) * | 2002-12-05 | 2009-02-17 | Boehringer Ingelheim Pharma Kg | Sequence specific DNA recombination in eukaryotic cells |
US20040115642A1 (en) * | 2002-12-12 | 2004-06-17 | Changlin Fu | Single-step methods for gene cloning |
US7078234B2 (en) | 2002-12-18 | 2006-07-18 | Monsanto Technology Llc | Maize embryo-specific promoter compositions and methods for use thereof |
US20040253620A1 (en) * | 2003-04-30 | 2004-12-16 | Invitrogen Corporation | Use of site specific recombination to prepare molecular markers |
EP1921152A1 (de) | 2003-05-05 | 2008-05-14 | Monsanto Technology, LLC | Transgene Pflanzen mit Glycin-Betain-spezifischem Promoter |
US20090203141A1 (en) * | 2003-05-15 | 2009-08-13 | Shi-Lung Lin | Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents |
US20060228800A1 (en) * | 2003-05-15 | 2006-10-12 | Shi-Lung Lin | Novel Transgenic Methods Using intronic RNA |
US9567591B2 (en) * | 2003-05-15 | 2017-02-14 | Mello Biotechnology, Inc. | Generation of human embryonic stem-like cells using intronic RNA |
EP1644538A4 (de) * | 2003-06-26 | 2006-11-08 | Invitrogen Corp | Verfahren und zusammensetzungen zum nachweis von promotoraktivität und zur expression von fusionsproteinen |
EP1687323A4 (de) * | 2003-08-08 | 2009-08-12 | Life Technologies Corp | Verfahren und zusammensetzungen zum nahtlosen klonieren von nukleinsäuremolekülen |
EP2272967B1 (de) | 2003-10-02 | 2015-12-23 | Monsanto Technology LLC | Sammeln von Kulturpflanzenverbesserungsmerkmalen bei transgenen Pflanzen |
US8304189B2 (en) | 2003-12-01 | 2012-11-06 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
GB2408980B (en) * | 2003-12-09 | 2006-06-07 | Nat Biolog Standards Board | Genetic reference materials |
US8071364B2 (en) * | 2003-12-24 | 2011-12-06 | Transgenrx, Inc. | Gene therapy using transposon-based vectors |
EP1711504A2 (de) | 2004-01-21 | 2006-10-18 | Molecular Probes Inc. | Derivate von cephalosporin und clavulansäure zum nachweis von beta-lactamase in einer probe |
AR047598A1 (es) | 2004-02-10 | 2006-01-25 | Monsanto Technology Llc | Semilla de maiz transgenica con mayor contenido de aminoacidos |
WO2005086654A2 (en) * | 2004-02-27 | 2005-09-22 | The Trustees Of Princeton University | Self-cleaving affinity tags and methods of use |
US9944713B2 (en) | 2004-11-24 | 2018-04-17 | Medicinal Bioconvergence Research Center | Antibody specific to the AIMP2-DX2 |
US8314290B2 (en) | 2004-12-21 | 2012-11-20 | Monsanto Technology Llc | Temporal regulation of gene expression by MicroRNAs |
EP2765189A1 (de) | 2004-12-21 | 2014-08-13 | Monsanto Technology LLC | Rekombinante DNA-Konstrukte und Verfahren zur Steuerung der Gen-Expression |
EP1874938B1 (de) * | 2005-04-19 | 2012-04-04 | BASF Plant Science GmbH | Endosperm-spezifische Expression und/oder Expression in keimenden Embryos monokotyledoner Pflanzen |
WO2006135974A1 (en) * | 2005-06-24 | 2006-12-28 | Australian Centre For Plant Functional Genomics Pty Ltd | Targeting vector |
KR20070009269A (ko) * | 2005-07-15 | 2007-01-18 | 한국생명공학연구원 | 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자 |
CA2625031C (en) | 2005-10-13 | 2016-07-19 | Monsanto Technology Llc | Methods for producing hybrid seed |
WO2007121467A2 (en) | 2006-04-18 | 2007-10-25 | Rutgers, The State University Of New Jersey | Compositions and methods for increasing transgene expression in the plastids of higher plants |
KR100762962B1 (ko) * | 2006-05-04 | 2007-10-04 | 한국과학기술원 | 게놈정보 및 인실리코 분석을 이용한 배양배지의 제조방법 |
US20100196336A1 (en) | 2006-05-23 | 2010-08-05 | Dongsu Park | Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods |
KR100780324B1 (ko) | 2006-07-28 | 2007-11-29 | 한국과학기술원 | 신규 순수 숙신산 생성 변이 미생물 및 이를 이용한 숙신산제조방법 |
ES2655700T3 (es) | 2006-08-31 | 2018-02-21 | Monsanto Technology, Llc | ARN pequeños en fase |
EP2074227A4 (de) | 2006-10-12 | 2010-03-10 | Monsanto Technology Llc | Pflanzen-mikro-rnas und verfahren zu ihrer verwendung |
KR20090119910A (ko) | 2007-02-16 | 2009-11-20 | 바스프 플랜트 사이언스 게엠베하 | 외떡잎 식물에서 배-특이적 발현의 조절을 위한 핵산 서열 |
EP2115141A4 (de) | 2007-02-20 | 2010-08-04 | Monsanto Technology Llc | miRNA AUS INVERTEBRATEN |
EP2135945B1 (de) * | 2007-03-14 | 2014-04-23 | Japan Science and Technology Agency | Verfahren zur doppelten regulation von transkription/abbau für ein protein mittels antibiotikum |
TWI382849B (zh) * | 2007-10-29 | 2013-01-21 | Mello Biotech Taiwan Co Ltd | 產生藉由基因靜默效應以用於皮膚維護之化妝品的方法以及編碼基因靜默效應物之基因重組核苷酸 |
WO2009114185A2 (en) | 2008-03-12 | 2009-09-17 | The Rockefeller University | Methods and compositions for translational profiling and molecular phenotyping |
US20090280103A1 (en) * | 2008-04-04 | 2009-11-12 | Martin Flueck | Regulation of muscle repair |
ES2563485T3 (es) | 2008-07-01 | 2016-03-15 | Monsanto Technology Llc | Construcciones de ADN recombinante y procedimientos para modular la expresión de un gen diana |
EP2342224A2 (de) * | 2008-09-25 | 2011-07-13 | TransGenRx, Inc. | Neue vektoren zur herstellung von interferon |
WO2010036976A2 (en) * | 2008-09-25 | 2010-04-01 | Transgenrx, Inc. | Novel vectors for production of antibodies |
WO2010036978A2 (en) * | 2008-09-25 | 2010-04-01 | Transgenrx, Inc. | Novel vectors for production of growth hormone |
US9150881B2 (en) * | 2009-04-09 | 2015-10-06 | Proteovec Holding, L.L.C. | Production of proteins using transposon-based vectors |
BRPI1011936A2 (pt) | 2009-07-02 | 2016-05-03 | Verdezyne Inc | métodos biológicos para a preparação de ácido adípico |
EP2451960A2 (de) * | 2009-07-09 | 2012-05-16 | Verdezyne, Inc. | Manipulierte mikroorganismen mit erhöhter gärungsaktivität |
US8889394B2 (en) * | 2009-09-07 | 2014-11-18 | Empire Technology Development Llc | Multiple domain proteins |
JPWO2012020574A1 (ja) * | 2010-08-13 | 2013-10-28 | 静岡県公立大学法人 | カビ類ポリケチド合成遺伝子の酵母での異種発現 |
US20120107815A1 (en) * | 2010-10-28 | 2012-05-03 | Arkray, Inc. | Polymorphism Detection Probe, Polymorphism Detection Method, Evaluation of Drug Efficacy, and Polymorphism Detection Kit |
US8695618B2 (en) | 2010-12-22 | 2014-04-15 | Carnegie Mellon University | 3D chemical pattern control in 2D fluidics devices |
JP6158170B2 (ja) * | 2011-04-27 | 2017-07-12 | アミリス, インコーポレイテッド | ゲノム修飾のための方法 |
US8343752B2 (en) | 2011-05-03 | 2013-01-01 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
US8728798B2 (en) | 2011-05-03 | 2014-05-20 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
US9738913B2 (en) | 2011-07-06 | 2017-08-22 | Verdezyne, Inc. | Biological methods for preparing a fatty dicarboxylic acid |
CN102296059A (zh) * | 2011-08-17 | 2011-12-28 | 石振宇 | 一种多段dna并行组装的方法 |
WO2013078433A1 (en) | 2011-11-23 | 2013-05-30 | University Of Hawaii | Auto-processing domains for polypeptide expression |
US9465519B2 (en) | 2011-12-21 | 2016-10-11 | Life Technologies Corporation | Methods and systems for in silico experimental designing and performing a biological workflow |
US9909151B2 (en) | 2012-12-19 | 2018-03-06 | Verdezyne, Inc. | Biological methods for preparing a fatty dicarboxylic acid |
SG11201504838YA (en) | 2012-12-19 | 2015-07-30 | Verdezyne Inc | Biological methods for preparing a fatty dicarboxylic acid |
BR112016003533B1 (pt) | 2013-08-19 | 2021-11-03 | Syngulon Sa | Célula microbiana geneticamente modificada e método para controlar o crescimento de uma segunda célula microbiana em um meio de cultura |
TWI638047B (zh) | 2014-04-09 | 2018-10-11 | 史基普研究協會 | 藉由核酸三磷酸酯轉運子將非天然或經修飾的核苷三磷酸酯輸入至細胞中 |
US9745611B2 (en) * | 2014-05-06 | 2017-08-29 | Genewiz Inc. | Methods and kits for identifying microorganisms in a sample |
FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
US20180148744A1 (en) | 2015-03-20 | 2018-05-31 | Verdezyne, Inc. | Biological methods for preparing 3-hydroxypropionic acid |
WO2017106767A1 (en) | 2015-12-18 | 2017-06-22 | The Scripps Research Institute | Production of unnatural nucleotides using a crispr/cas9 system |
CN106397598B (zh) * | 2016-02-23 | 2020-07-14 | 上海交通大学 | 多价多特异性抗体及免疫杂合蛋白的表达和制备方法 |
EP4163293A1 (de) | 2016-06-24 | 2023-04-12 | The Scripps Research Institute | Neuartiger nukleosid-triphosphat-transporter und verwendungen davon |
WO2019014267A1 (en) | 2017-07-11 | 2019-01-17 | Synthorx, Inc. | INCORPORATION OF NON-NATURAL NUCLEOTIDES AND ASSOCIATED METHODS |
US11174488B2 (en) | 2017-07-13 | 2021-11-16 | Radici Chimica S.P.A. | Biological methods for modifying cellular carbon flux |
JP2020529976A (ja) | 2017-08-03 | 2020-10-15 | シンソークス,インク. | 自己免疫疾患の処置のためのサイトカイン抱合体 |
JP2021506328A (ja) | 2017-12-19 | 2021-02-22 | シンギュロン・ソシエテ・アノニム | 発酵プロセス |
WO2020060948A1 (en) | 2018-09-17 | 2020-03-26 | Levadura Biotechnology, Inc. | Production of cannabinoids in yeast using a fatty acid feedstock |
US20200246467A1 (en) | 2019-02-06 | 2020-08-06 | Synthorx, Inc. | Il-2 conjugates and methods of use thereof |
CN114207129A (zh) | 2019-06-14 | 2022-03-18 | 斯克利普斯研究所 | 用于在半合成生物体中复制、转录和翻译的试剂和方法 |
WO2021022308A2 (en) * | 2019-08-01 | 2021-02-04 | The Regents Of The University Of California | Non-viral transgenesis |
Family Cites Families (189)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4873191A (en) | 1981-06-12 | 1989-10-10 | Ohio University | Genetic transformation of zygotes |
US4626505A (en) | 1984-02-24 | 1986-12-02 | E. I. Du Pont De Nemours And Company | Selectable markers for yeast transformation |
US4673640A (en) | 1984-04-30 | 1987-06-16 | Biotechnica International, Inc. | Regulated protein production using site-specific recombination |
US4736866A (en) | 1984-06-22 | 1988-04-12 | President And Fellows Of Harvard College | Transgenic non-human mammals |
US4808537A (en) | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
US4743546A (en) | 1985-02-13 | 1988-05-10 | Biotechnica International, Inc. | Controlled gene excision |
US5200333A (en) | 1985-03-01 | 1993-04-06 | New England Biolabs, Inc. | Cloning restriction and modification genes |
IT8520964V0 (it) | 1985-03-01 | 1985-03-01 | Alfa Romeo Spa | Contenitore per il liquido lavavetri di un autoveicolo. |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
DE3688920D1 (de) | 1985-07-03 | 1993-09-30 | Genencor Int | Hybride Polypeptide und Verfahren zu deren Herstellung. |
US4980286A (en) | 1985-07-05 | 1990-12-25 | Whitehead Institute For Biomedical Research | In vivo introduction and expression of foreign genetic material in epithelial cells |
US6121043A (en) | 1985-09-06 | 2000-09-19 | Syntro Corporation | Recombinant herpesvirus of turkeys comprising a foreign DNA inserted into a non-essential region of the herpesvirus of turkeys genome |
CA1293460C (en) | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US5149639A (en) | 1986-03-24 | 1992-09-22 | Abbott Laboratories | Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production |
US4874748A (en) | 1986-03-24 | 1989-10-17 | Abbott Laboratories | Cloning vectors for streptomyces and use thereof in macrolide antibiotic production |
US4959217A (en) | 1986-05-22 | 1990-09-25 | Syntex (U.S.A.) Inc. | Delayed/sustained release of macromolecules |
US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
US4962022A (en) | 1986-09-22 | 1990-10-09 | Becton Dickinson And Company | Storage and use of liposomes |
JPS6427477A (en) * | 1987-04-01 | 1989-01-30 | Takeda Chemical Industries Ltd | Dna and use thereof |
US4960707A (en) | 1987-08-17 | 1990-10-02 | Associated Universities, Inc. | Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae |
US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
US5063155A (en) | 1988-03-28 | 1991-11-05 | Eli Lilly And Company | Method for producing 2"'-o-demethyltylosin |
US5378618A (en) | 1988-04-15 | 1995-01-03 | E. I. Du Pont De Nemours And Company | Vitro headful packaging system for cloning DNA fragments as large as 95kb |
US5139942A (en) | 1988-05-19 | 1992-08-18 | New England Biolabs, Inc. | Method for producing the nde i restriction endonuclease and methylase |
US5098837A (en) | 1988-06-07 | 1992-03-24 | Eli Lilly And Company | Macrolide biosynthetic genes for use in streptomyces and other organisms |
US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
GB8822228D0 (en) | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
US5334375A (en) | 1988-12-29 | 1994-08-02 | Colgate Palmolive Company | Antibacterial antiplaque oral composition |
DE3901681A1 (de) | 1989-01-21 | 1990-07-26 | Behringwerke Ag | Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung |
US5102797A (en) | 1989-05-26 | 1992-04-07 | Dna Plant Technology Corporation | Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system |
US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US6309822B1 (en) | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
US5270179A (en) | 1989-08-10 | 1993-12-14 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity |
US5047342A (en) | 1989-08-10 | 1991-09-10 | Life Technologies, Inc. | Cloning and expression of T5 DNA polymerase |
US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
US5436146A (en) | 1989-09-07 | 1995-07-25 | The Trustees Of Princeton University | Helper-free stocks of recombinant adeno-associated virus vectors |
US5252743A (en) | 1989-11-13 | 1993-10-12 | Affymax Technologies N.V. | Spatially-addressable immobilization of anti-ligands on surfaces |
US5658772A (en) | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
ATE201047T1 (de) | 1990-01-08 | 2001-05-15 | Stratagene Inc | Neue wirtsorganismen zur klonierung |
US5283173A (en) | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
US5082784A (en) | 1990-03-20 | 1992-01-21 | Life Technologies, Inc. | Cloned kpni restriction-modification system |
US5192675A (en) | 1990-03-20 | 1993-03-09 | Life Technologies, Inc. | Cloned KpnI restriction-modification system |
US5451513A (en) | 1990-05-01 | 1995-09-19 | The State University of New Jersey Rutgers | Method for stably transforming plastids of multicellular plants |
US5098839A (en) | 1990-05-10 | 1992-03-24 | New England Biolabs, Inc. | Type ii restriction endonuclease obtainable from pseudomonas alcaligenes and a process for producing the same |
US5147800A (en) | 1990-06-08 | 1992-09-15 | Life Technologies, Inc. | Host expressing ngoaiii restriction endonuclease and modification methylase from neisseria |
US5179015A (en) | 1990-07-23 | 1993-01-12 | New England Biolabs, Inc. | Heterospecific modification as a means to clone restriction genes |
ES2134198T3 (es) | 1990-09-28 | 1999-10-01 | Hoffmann La Roche | Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas. |
AU660629B2 (en) | 1990-10-01 | 1995-07-06 | University Of Connecticut, The | Targeting viruses and cells for selective internalization by cells |
US5227288A (en) | 1990-10-01 | 1993-07-13 | Blattner Frederick R | DNA sequencing vector with reversible insert |
WO1992006188A2 (en) | 1990-10-05 | 1992-04-16 | Barnes Wayne M | Thermostable dna polymerase |
US5202248A (en) | 1990-11-02 | 1993-04-13 | New England Biolabs, Inc. | Method for cloning and producing the nco i restriction endonuclease and methylase |
US5286632A (en) | 1991-01-09 | 1994-02-15 | Jones Douglas H | Method for in vivo recombination and mutagenesis |
WO1993013663A1 (en) | 1992-01-17 | 1993-07-22 | Abbott Laboratories | Method of directing biosynthesis of specific polyketides |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5328984A (en) | 1991-03-04 | 1994-07-12 | The United States As Represented By The Department Of Health & Human Services | Recombinant chimeric proteins deliverable across cellular membranes into cytosol of target cells |
WO1992015694A1 (en) | 1991-03-08 | 1992-09-17 | The Salk Institute For Biological Studies | Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor |
US5763170A (en) | 1991-04-16 | 1998-06-09 | Amersham International Plc | Method for forming an array of biological particles |
ATE199647T1 (de) | 1991-05-14 | 2001-03-15 | Univ Connecticut | Gerichtete abgabe von genen, die immunogene proteine kodieren |
US5858657A (en) | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
DE69230142T2 (de) * | 1991-05-15 | 2000-03-09 | Cambridge Antibody Tech | Verfahren zur herstellung von spezifischen bindungspaargliedern |
ATE195656T1 (de) | 1991-06-05 | 2000-09-15 | Univ Connecticut | Zielgerichtete freisetzung von genen, die sekretorische proteine kodieren |
US5412087A (en) | 1992-04-24 | 1995-05-02 | Affymax Technologies N.V. | Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces |
WO1993014188A1 (en) | 1992-01-17 | 1993-07-22 | The Regents Of The University Of Michigan | Targeted virus |
WO1993015191A1 (en) | 1992-01-24 | 1993-08-05 | Life Technologies, Inc. | Modulation of enzyme activities in the in vivo cloning of dna |
ATE201236T1 (de) | 1992-02-26 | 2001-06-15 | Zeneca Mogen B V | Zur ortsspezifischen rekomination fähige agrobakterium stämme |
US5733743A (en) | 1992-03-24 | 1998-03-31 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
AU3940293A (en) | 1992-04-03 | 1993-11-08 | Alexander T. YOUNG | Gene therapy using targeted viral vectors |
US5231021A (en) | 1992-04-10 | 1993-07-27 | Life Technologies, Inc. | Cloning and expressing restriction endonucleases and modification methylases from xanthomonas |
BE1006085A3 (fr) | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
US5348886A (en) | 1992-09-04 | 1994-09-20 | Monsanto Company | Method of producing recombinant eukaryotic viruses in bacteria |
US6225121B1 (en) | 1992-09-14 | 2001-05-01 | Institute Of Molecular Biology And Biotechnology/Forth | Eukaryotic transposable element |
JPH08504320A (ja) | 1992-09-25 | 1996-05-14 | コモンウエルス・サイエンティフィック・アンド・インダストリアル・リサーチ・オーガニゼーション | 標的結合性ポリペプチド |
CA2146747C (en) | 1992-10-09 | 2006-12-19 | Brian A. Naughton | Liver reserve cells |
AU680459B2 (en) | 1992-12-03 | 1997-07-31 | Genzyme Corporation | Gene therapy for cystic fibrosis |
JP3720353B2 (ja) | 1992-12-04 | 2005-11-24 | メディカル リサーチ カウンシル | 多価および多重特異性の結合タンパク質、それらの製造および使用 |
US5248605A (en) | 1992-12-07 | 1993-09-28 | Life Technologies, Inc. | Cloning and expressing restriction endonucleases from haemophilus |
US5334575A (en) | 1992-12-17 | 1994-08-02 | Eastman Kodak Company | Dye-containing beads for laser-induced thermal dye transfer |
US5312746A (en) | 1993-01-08 | 1994-05-17 | Life Technologies, Inc. | Cloning and expressing restriction endonucleases and modification methylases from caryophanon |
US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
US5334526A (en) | 1993-05-28 | 1994-08-02 | Life Technologies, Inc. | Cloning and expression of AluI restriction endonuclease |
US6120764A (en) | 1993-06-24 | 2000-09-19 | Advec, Inc. | Adenoviruses for control of gene expression |
US6140087A (en) | 1993-06-24 | 2000-10-31 | Advec, Inc. | Adenovirus vectors for gene therapy |
US5919676A (en) | 1993-06-24 | 1999-07-06 | Advec, Inc. | Adenoviral vector system comprising Cre-loxP recombination |
US5837832A (en) | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
EP0632054A1 (de) | 1993-06-28 | 1995-01-04 | European Molecular Biology Laboratory | Regulation stellenspezifischer Rekombination mittels stellungsspezifischer Rekombinase/Kernrezeptor-Fusionsproteine |
US5843744A (en) | 1993-07-08 | 1998-12-01 | Ecogen Inc. | Bacillus thuringiensis Tn5401 proteins |
US5441884A (en) | 1993-07-08 | 1995-08-15 | Ecogen Inc. | Bacillus thuringiensis transposon TN5401 |
US5672491A (en) | 1993-09-20 | 1997-09-30 | The Leland Stanford Junior University | Recombinant production of novel polyketides |
CA2171629A1 (en) | 1993-09-20 | 1995-03-30 | Chaitan Khosla | Recombinant production of novel polyketides |
US5712146A (en) | 1993-09-20 | 1998-01-27 | The Leland Stanford Junior University | Recombinant combinatorial genetic library for the production of novel polyketides |
US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
US5470740A (en) | 1993-11-04 | 1995-11-28 | Life Technologies, Inc. | Cloned NsiI restriction-modification system |
US5556772A (en) | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
US5470727A (en) | 1993-12-21 | 1995-11-28 | Celtrix Pharmaceuticals, Inc. | Chromosomal expression of non-bacterial genes in bacterial cells |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
WO1995032225A1 (en) | 1994-05-23 | 1995-11-30 | The Salk Institute For Biological Studies | Method for site-specific integration of nucleic acids and related products |
US5723765A (en) | 1994-08-01 | 1998-03-03 | Delta And Pine Land Co. | Control of plant gene expression |
US5589618A (en) | 1994-09-01 | 1996-12-31 | University Of Florida | Materials and methods for increasing corn seed weight |
US5534428A (en) | 1994-09-06 | 1996-07-09 | Life Technologies, Inc. | Cloned Ssti/SacI restriction-modification system |
US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
US5614365A (en) | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
AU716045B2 (en) | 1994-11-28 | 2000-02-17 | Genetic Therapy, Inc. | Vectors for tissue-specific replication |
US5998205A (en) | 1994-11-28 | 1999-12-07 | Genetic Therapy, Inc. | Vectors for tissue-specific replication |
FR2727689A1 (fr) | 1994-12-01 | 1996-06-07 | Transgene Sa | Nouveau procede de preparation d'un vecteur viral |
US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US5981177A (en) | 1995-01-25 | 1999-11-09 | Demirjian; David C. | Protein fusion method and constructs |
US5599695A (en) | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
US5814300A (en) * | 1995-03-03 | 1998-09-29 | Cephalon, Inc. | Gene-targeted non-human mammals deficient in the SOD-1 gene |
US5695971A (en) | 1995-04-07 | 1997-12-09 | Amresco | Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production |
US5965796A (en) | 1995-04-21 | 1999-10-12 | University Of Georgia Research Foundation Inc. | Metal resistance sequences and transgenic plants |
US5877274A (en) | 1995-06-02 | 1999-03-02 | University Of British Columbia | Antimicrobial cationic peptides |
US6964861B1 (en) | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
WO1996040724A1 (en) * | 1995-06-07 | 1996-12-19 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
US7670823B1 (en) | 1999-03-02 | 2010-03-02 | Life Technologies Corp. | Compositions for use in recombinational cloning of nucleic acids |
US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
EP0836649B1 (de) | 1995-07-06 | 2004-12-29 | The Leland Stanford Junior University | Zellfreie synthese von polyketiden |
US5801030A (en) | 1995-09-01 | 1998-09-01 | Genvec, Inc. | Methods and vectors for site-specific recombination |
JP2000502882A (ja) | 1995-09-08 | 2000-03-14 | ライフ・テクノロジーズ・インコーポレイテッド | サーモトガ由来のクローン化dnaポリメラーゼ類およびそれらの変異体 |
US5770435A (en) | 1995-11-02 | 1998-06-23 | University Of Chicago | Mutant E. coli strain with increased succinic acid production |
CA2239366A1 (en) | 1996-01-05 | 1997-07-17 | Genetic Therapy, Inc. | Recombinase-mediated generation of adenoviral vectors |
US6228646B1 (en) | 1996-03-07 | 2001-05-08 | The Regents Of The University Of California | Helper-free, totally defective adenovirus for gene therapy |
US5928914A (en) | 1996-06-14 | 1999-07-27 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Methods and compositions for transforming cells |
US5710248A (en) | 1996-07-29 | 1998-01-20 | University Of Iowa Research Foundation | Peptide tag for immunodetection and immunopurification |
EP2264045B1 (de) | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren |
US6025192A (en) | 1996-09-20 | 2000-02-15 | Cold Spring Harbor Laboratory | Modified retroviral vectors |
JP2002511732A (ja) * | 1996-10-11 | 2002-04-16 | ザ・テキサス・エイ・アンド・エム・ユニヴァーシティ・システム | トランスジェニック動物を発生させるための組成物および方法 |
US6066778A (en) | 1996-11-06 | 2000-05-23 | The Regents Of The University Of Michigan | Transgenic mice expressing APC resistant factor V |
US6083697A (en) | 1996-11-14 | 2000-07-04 | Affymetrix, Inc. | Chemical amplification for the synthesis of patterned arrays |
US6110372A (en) | 1996-12-17 | 2000-08-29 | Perriello; Felix Anthony | Bioremediation of petroleum pollutants with alkane-utilizing bacteria |
US6033883A (en) | 1996-12-18 | 2000-03-07 | Kosan Biosciences, Inc. | Production of polyketides in bacteria and yeast |
US6303301B1 (en) | 1997-01-13 | 2001-10-16 | Affymetrix, Inc. | Expression monitoring for gene function identification |
US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
WO1998047912A1 (en) | 1997-04-22 | 1998-10-29 | Life Technologies, Inc. | Methods for the production of aslv reverse transcriptases composed of multiple subunits |
JP2001515234A (ja) | 1997-07-25 | 2001-09-18 | アフィメトリックス インコーポレイテッド | 多型性データベースを提供するためのシステム |
US5989872A (en) | 1997-08-12 | 1999-11-23 | Clontech Laboratories, Inc. | Methods and compositions for transferring DNA sequence information among vectors |
US5888795A (en) | 1997-09-09 | 1999-03-30 | Becton, Dickinson And Company | Thermostable uracil DNA glycosylase and methods of use |
US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
CN101125873A (zh) | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
US7351578B2 (en) * | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
DE69836092T2 (de) | 1997-10-24 | 2007-05-10 | Invitrogen Corp., Carlsbad | Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen |
DE69833457T2 (de) * | 1997-11-18 | 2006-09-14 | Pioneer Hi-Bred International, Inc. | Neuartige methode zur integration von fremd-dna ins eukaryotische genom |
US5874259A (en) | 1997-11-21 | 1999-02-23 | Wisconsin Alumni Research Foundation | Conditionally amplifiable BAC vector |
US5994136A (en) | 1997-12-12 | 1999-11-30 | Cell Genesys, Inc. | Method and means for producing high titer, safe, recombinant lentivirus vectors |
US6333155B1 (en) | 1997-12-19 | 2001-12-25 | Affymetrix, Inc. | Exploiting genomics in the search for new drugs |
US6150147A (en) | 1998-02-06 | 2000-11-21 | Affymetrix, Inc. | Biological array fabrication methods with reduction of static charge |
US6436707B1 (en) | 1998-03-27 | 2002-08-20 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
US6080576A (en) | 1998-03-27 | 2000-06-27 | Lexicon Genetics Incorporated | Vectors for gene trapping and gene activation |
EP1114148A4 (de) | 1998-08-28 | 2004-12-22 | Invitrogen Corp | System zur schnellen manipulation von nukleinsäuresequenzen. |
US6576463B1 (en) | 1999-01-15 | 2003-06-10 | The Regents Of The University Of California | Hybrid vectors for gene therapy |
US6120814A (en) | 1999-02-22 | 2000-09-19 | Nature's One, Inc. | Organic nutritional formula |
EP1165775A2 (de) | 1999-03-05 | 2002-01-02 | Maxygen, Inc. | Rekombination von insertionsmodifizierten nucleinsäuren |
US6410317B1 (en) | 1999-07-14 | 2002-06-25 | Clontech Laboratories, Inc. | Recombinase-based methods for producing expression vectors and compositions for use in practicing the same |
JP2003509060A (ja) | 1999-09-17 | 2003-03-11 | ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ | 逆トランスフェクション方法 |
NZ518187A (en) | 1999-11-04 | 2002-12-20 | Genentech Inc | Reversed-phase HPLC assay for plasminogen activators |
JP4729222B2 (ja) | 1999-12-10 | 2011-07-20 | ライフ テクノロジーズ コーポレーション | 組換えクローニングにおける独自の選択性を有する複数の組換え部位の使用 |
WO2001062892A2 (en) | 2000-02-25 | 2001-08-30 | Stratagene | Method for ligating nucleic acids and molecular cloning |
US6451602B1 (en) | 2000-03-02 | 2002-09-17 | Isis Pharmaceuticals, Inc. | Antisense modulation of PARP expression |
CA2403397A1 (en) | 2000-03-16 | 2001-09-20 | Genetica, Inc. | Methods and compositions for rna interference |
US20020068290A1 (en) | 2000-05-31 | 2002-06-06 | Timur Yarovinsky | Topoisomerase activated oligonucleotide adaptors and uses therefor |
JP5043277B2 (ja) | 2000-08-21 | 2012-10-10 | ライフ テクノロジーズ コーポレーション | 分子クローニング法および使用試薬 |
US7198924B2 (en) * | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US20030135888A1 (en) * | 2001-09-26 | 2003-07-17 | Tong Zhu | Genes that are modulated by posttranscriptional gene silencing |
JP4210769B2 (ja) * | 2001-11-22 | 2009-01-21 | 独立行政法人産業技術総合研究所 | Gatewayエントリークローンの作製方法 |
US7294504B1 (en) * | 2001-12-27 | 2007-11-13 | Allele Biotechnology & Pharmaceuticals, Inc. | Methods and compositions for DNA mediated gene silencing |
WO2004009768A2 (en) * | 2002-07-18 | 2004-01-29 | Invitrogen Corporation | Viral vectors containing recombination sites |
EP1644538A4 (de) * | 2003-06-26 | 2006-11-08 | Invitrogen Corp | Verfahren und zusammensetzungen zum nachweis von promotoraktivität und zur expression von fusionsproteinen |
EP1687323A4 (de) * | 2003-08-08 | 2009-08-12 | Life Technologies Corp | Verfahren und zusammensetzungen zum nahtlosen klonieren von nukleinsäuremolekülen |
WO2005054427A2 (en) * | 2003-10-22 | 2005-06-16 | Invitrogen Corporation | Target sequences for synthetic molecules |
US8972161B1 (en) | 2005-11-17 | 2015-03-03 | Invent.Ly, Llc | Power management systems and devices |
-
2000
- 2000-12-11 JP JP2001544380A patent/JP4729222B2/ja not_active Expired - Lifetime
- 2000-12-11 NZ NZ530816A patent/NZ530816A/en not_active IP Right Cessation
- 2000-12-11 DE DE60038572T patent/DE60038572T2/de not_active Expired - Lifetime
- 2000-12-11 WO PCT/US2000/033546 patent/WO2001042509A1/en active IP Right Grant
- 2000-12-11 DK DK00984184T patent/DK1250453T3/da active
- 2000-12-11 AT AT00984184T patent/ATE391789T1/de not_active IP Right Cessation
- 2000-12-11 EP EP08153538A patent/EP1978098A3/de not_active Withdrawn
- 2000-12-11 AU AU20851/01A patent/AU785483B2/en not_active Ceased
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- 2000-12-11 CN CN00818077.6A patent/CN100587068C/zh not_active Expired - Lifetime
- 2000-12-11 NZ NZ539430A patent/NZ539430A/en unknown
- 2000-12-11 EP EP00984184A patent/EP1250453B1/de not_active Expired - Lifetime
- 2000-12-11 CN CN200510103730.5A patent/CN1757724B/zh not_active Expired - Lifetime
- 2000-12-11 EP EP10151112A patent/EP2210948A3/de not_active Withdrawn
- 2000-12-11 US US09/732,914 patent/US7393632B2/en not_active Expired - Lifetime
- 2000-12-11 CA CA002392753A patent/CA2392753A1/en not_active Abandoned
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Also Published As
Publication number | Publication date |
---|---|
AU785483B2 (en) | 2007-09-20 |
DE60038572D1 (de) | 2008-05-21 |
CN1468317A (zh) | 2004-01-14 |
EP1978098A2 (de) | 2008-10-08 |
EP2210948A3 (de) | 2010-10-06 |
EP1250453B1 (de) | 2008-04-09 |
WO2001042509A1 (en) | 2001-06-14 |
AU2007221891A1 (en) | 2007-11-01 |
JP4729222B2 (ja) | 2011-07-20 |
NZ530816A (en) | 2005-10-28 |
JP2004500061A (ja) | 2004-01-08 |
WO2001042509A9 (en) | 2002-05-30 |
AU2085101A (en) | 2001-06-18 |
CN1757724A (zh) | 2006-04-12 |
US20020007051A1 (en) | 2002-01-17 |
NZ539430A (en) | 2006-09-29 |
EP1250453A1 (de) | 2002-10-23 |
EP2210948A2 (de) | 2010-07-28 |
CN1757724B (zh) | 2014-06-11 |
HK1062034A1 (en) | 2004-10-15 |
CA2392753A1 (en) | 2001-06-14 |
US7393632B2 (en) | 2008-07-01 |
DK1250453T3 (da) | 2008-08-11 |
EP1978098A3 (de) | 2009-02-18 |
NZ519217A (en) | 2004-03-26 |
CN100587068C (zh) | 2010-02-03 |
EP1250453A4 (de) | 2004-04-07 |
DE60038572T2 (de) | 2009-04-30 |
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