JPH08504320A - 標的結合性ポリペプチド - Google Patents
標的結合性ポリペプチドInfo
- Publication number
- JPH08504320A JPH08504320A JP6508515A JP50851594A JPH08504320A JP H08504320 A JPH08504320 A JP H08504320A JP 6508515 A JP6508515 A JP 6508515A JP 50851594 A JP50851594 A JP 50851594A JP H08504320 A JPH08504320 A JP H08504320A
- Authority
- JP
- Japan
- Prior art keywords
- target
- polypeptide
- binding
- antibody
- binding polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1018—Orthomyxoviridae, e.g. influenza virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.標的結合性ポリペプチドであって、 a)安定なコアポリペプチド領域(SCR)と、 b)少なくとも一つの標的結合領域(TBR) とを具備し、 前記標的結合領域は、前記SCRに共有結合体に結合しており、また標的に対 する特異性、親和性または結合活性を改変するために、任意に成熟プロセスを受 けているポリペプチド。 2.請求項1に記載のポリペプチドであって、自己会合して安定な二量体、凝 集体またはアレイを形成することができるポリペプチド。 3.請求項1または2に記載のポリペプチドであって、前記SCRおよびTB Rがリンカー成分によって結合されているポリペプチド。 4.請求項1〜3の何れか1項に記載のポリペプチドであって、二官能性また は多官能性であるポリペプチド。 5.請求項1〜4の何れか1項に記載のポリペプチドであって、前記SCRが ヒト起源であり、また血清中に存在するか若しくは細胞表面に表現されるタイプ のタンパクであるポリペプチド。 6.請求項1〜5の何れか1項に記載のポリペプチドであって、前記TBRが 、グリコホリンもしくは他の赤血球細胞表面タンパク、インフルエンザウイルス ・ノイラミニダーゼ、 ウイルス抗原、抗体もしくは他のIgGファミリーのメンバー、腫瘍増殖因子α (TGF−α)、腫瘍マーカー、細胞表面タンパクおよび白血病阻害因子(LI F)からなる群から選択される標的に結合することができるポリペプチド。 7.請求項1〜6の何れか1項に記載の標的結合性ポリペプチドであって、、 修飾抗体もしくは抗体フラグメント、scFvフラグメント、修飾CD8分子、 および抗体分子もしくはそのフラグメントとCD8との組合せを含む、免疫グロ ブリンスーパーファミリーに対して相同性を有するポリペプチド。 8.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドをコードする DNA構築物。 9.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドをコードする DNA構築物を製造する方法であって、標的結合性ポリペプチドコードするDN Aを、突然変異および選別の一以上のサイクルに供して、改変された親和性、特 異性または結合活性の特性をもった標的結合性ポリペプチドをコードするDNA のサブポピュレーションを得ることを具備した方法。 10.請求項9に記載の方法であって、標的結合性ポリペプチドをコードする 前記DNAが、複製の能力のある要素または表現ベクター内に存在する方法。 11.請求項10に記載の方法であって、前記表現ベクターが、バクテリオフ ァージ、繊維状バクテリオファージ、ウイルス、バクテリア、酵母、粘菌、また は哺乳類細胞からな る群から選択される方法。 12.請求項10に記載の方法であて、前記表現ベクターがpHFAである方 法。 13.請求項12に記載の方法であって、突然変異が大腸菌の突然変異株を用 いて行なわれる方法。 14.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドを製造する 方法であって、 a)一以上の所望の標的結合性ポリペプチドのフレームワーク構造をコードす るDNAを、ポリメラーゼチェイン反応によって単離する工程と、 b)該DNAを一以上の表現ベクターに挿入する工程と、 c)所望の特異性、結合活性または親和性の標的結合性ポリペプチドを表現す る表現ベクターのサブポピュレーションを選別することと、 d)改変された親和性、特異性または結合活性の特性を有する標的結合性ポリ ペプチドを表現する表現ベクターのサブポピュレーションを得るために、前記該 選別されたサブポピュレーションを、突然変異誘発および選別の一以上のサイク ルに供する工程と、 e)改変された標的結合性ポリペプチドをコードするDNAを、高レベル発現 ベクターに挿入する工程と、 f)上記の高レベル発現ベクターを適切な発現ホスト中に導入する工程と、 g)前記標的結合性タンパクを発現させる工程と、 h)こうして産生されたタンパクを単離する工程とを具備 した方法。 14.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドと、薬学的 に許容され得る担体とを含有する薬剤組成物。 15.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドと、稀釈剤 とを含有する診断試薬。 16.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドの診断にお ける使用。 17.請求項1〜7の何れか1項に記載の標的結合性ポリペプチドの医薬とし ての使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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AUPL497392 | 1992-09-25 | ||
AU4973 | 1992-09-25 | ||
PCT/AU1993/000491 WO1994007921A1 (en) | 1992-09-25 | 1993-09-24 | Target binding polypeptide |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2008302527A Division JP2009148252A (ja) | 1992-09-25 | 2008-11-27 | 標的結合性ポリペプチド |
Publications (1)
Publication Number | Publication Date |
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JPH08504320A true JPH08504320A (ja) | 1996-05-14 |
Family
ID=3776442
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JP6508515A Withdrawn JPH08504320A (ja) | 1992-09-25 | 1993-09-24 | 標的結合性ポリペプチド |
JP2008302527A Pending JP2009148252A (ja) | 1992-09-25 | 2008-11-27 | 標的結合性ポリペプチド |
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JP2008302527A Pending JP2009148252A (ja) | 1992-09-25 | 2008-11-27 | 標的結合性ポリペプチド |
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US (1) | US5844094A (ja) |
EP (2) | EP1550729B1 (ja) |
JP (2) | JPH08504320A (ja) |
CA (1) | CA2145278C (ja) |
DE (1) | DE69334287D1 (ja) |
HK (1) | HK1078110A1 (ja) |
SG (1) | SG41929A1 (ja) |
WO (1) | WO1994007921A1 (ja) |
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