ATE460472T1 - Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen - Google Patents

Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen

Info

Publication number
ATE460472T1
ATE460472T1 AT01996076T AT01996076T ATE460472T1 AT E460472 T1 ATE460472 T1 AT E460472T1 AT 01996076 T AT01996076 T AT 01996076T AT 01996076 T AT01996076 T AT 01996076T AT E460472 T1 ATE460472 T1 AT E460472T1
Authority
AT
Austria
Prior art keywords
methods
nucleic acid
acid molecules
compositions
molecules
Prior art date
Application number
AT01996076T
Other languages
English (en)
Inventor
Jonathan Chesnut
John Carrino
Louis Leong
Knut Madden
Martin Gleeson
James Fan
Michael Brasch
David Cheo
James Hartley
Devon Byrd
Gary Temple
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/732,914 external-priority patent/US7393632B2/en
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE460472T1 publication Critical patent/ATE460472T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Hall/Mr Elements (AREA)
AT01996076T 2000-12-08 2001-12-07 Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen ATE460472T1 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US25451000P 2000-12-08 2000-12-08
US09/732,914 US7393632B2 (en) 1999-12-10 2000-12-11 Use of multiple recombination sites with unique specificity in recombinational cloning
US31890201P 2001-09-14 2001-09-14
US32609201P 2001-09-28 2001-09-28
US33312401P 2001-11-27 2001-11-27
PCT/US2001/045773 WO2002046372A1 (en) 2000-12-08 2001-12-07 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Publications (1)

Publication Number Publication Date
ATE460472T1 true ATE460472T1 (de) 2010-03-15

Family

ID=27540352

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01996076T ATE460472T1 (de) 2000-12-08 2001-12-07 Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen

Country Status (9)

Country Link
EP (2) EP1349917B1 (de)
JP (2) JP4394878B2 (de)
CN (1) CN100357427C (de)
AT (1) ATE460472T1 (de)
AU (2) AU2715302A (de)
CA (1) CA2430947A1 (de)
DE (1) DE60141533D1 (de)
NZ (1) NZ526803A (de)
WO (1) WO2002046372A1 (de)

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US7160702B2 (en) 2002-07-26 2007-01-09 Shuwei Yang Methods and nucleic acid vectors for rapid expression and screening of CDNA clones
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US20050176065A1 (en) 2003-10-22 2005-08-11 Invitrogen Corporation Target sequences for synthetic molecules
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
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CN105039375B (zh) * 2015-03-11 2019-02-05 戚智青 一种利用蛋白ihf构建克隆载体的方法
TWI678213B (zh) 2015-07-22 2019-12-01 美商史倍壯製藥公司 用於長春新鹼硫酸鹽脂質體注射之即可使用的調配物
JP7015551B2 (ja) 2016-02-23 2022-02-15 ソーク インスティテュート フォー バイオロジカル スタディーズ ウイルス動態への影響を最小限にするための治療用アデノウイルスにおける外因性遺伝子発現
EP3390428B1 (de) 2016-02-23 2019-09-25 Salk Institute for Biological Studies Test mit hohem durchsatz zur messung der adenovirusreplikationskinetik
JP7794421B2 (ja) 2016-12-12 2026-01-06 ソーク インスティテュート フォー バイオロジカル スタディーズ 腫瘍を標的にする合成アデノウイルスおよびその使用
BR112020019942A2 (pt) 2018-04-09 2021-01-26 Salk Institute For Biological Studies composições de adenovírus oncolítico com propriedades de replicação aprimoradas
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CN117092084B (zh) * 2023-10-20 2024-01-12 浙江迪福润丝生物科技有限公司 Wnv蛋白酶抑制剂的筛选方法和抑制效果评价方法

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Also Published As

Publication number Publication date
EP1349917A4 (de) 2004-09-08
CN1489627A (zh) 2004-04-14
JP4394878B2 (ja) 2010-01-06
AU2715302A (en) 2002-06-18
JP2004522428A (ja) 2004-07-29
EP1349917B1 (de) 2010-03-10
EP1349917A1 (de) 2003-10-08
EP2105496B1 (de) 2013-02-20
CA2430947A1 (en) 2002-06-13
JP2009219497A (ja) 2009-10-01
NZ526803A (en) 2005-02-25
JP5191953B2 (ja) 2013-05-08
AU2002227153B2 (en) 2008-01-24
DE60141533D1 (de) 2010-04-22
CN100357427C (zh) 2007-12-26
EP2105496A1 (de) 2009-09-30
WO2002046372A9 (en) 2004-04-29
WO2002046372A1 (en) 2002-06-13

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