WO2003103600A3 - Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites - Google Patents

Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites Download PDF

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Publication number
WO2003103600A3
WO2003103600A3 PCT/US2003/018036 US0318036W WO03103600A3 WO 2003103600 A3 WO2003103600 A3 WO 2003103600A3 US 0318036 W US0318036 W US 0318036W WO 03103600 A3 WO03103600 A3 WO 03103600A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
acid molecules
compositions
molecules
Prior art date
Application number
PCT/US2003/018036
Other languages
French (fr)
Other versions
WO2003103600A2 (en
Inventor
Jonathan D Chesnut
John Carrino
Louis Leong
Knut Madden
Martin Gleeson
James Fan
Michael A Brasch
David Cheo
James L Hartley
Devon R N Byrd
Gary F Temple
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Priority to AU2003273995A priority Critical patent/AU2003273995A1/en
Priority to EP03741892A priority patent/EP1539998A4/en
Publication of WO2003103600A2 publication Critical patent/WO2003103600A2/en
Priority to US10/792,035 priority patent/US7198924B2/en
Publication of WO2003103600A3 publication Critical patent/WO2003103600A3/en
Priority to US11/612,445 priority patent/US8030066B2/en
Priority to US13/219,440 priority patent/US20130004996A1/en
Priority to US15/060,536 priority patent/US20160251664A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
PCT/US2003/018036 2000-08-21 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites WO2003103600A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2003273995A AU2003273995A1 (en) 2002-06-05 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP03741892A EP1539998A4 (en) 2002-06-05 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US10/792,035 US7198924B2 (en) 2000-12-11 2004-03-04 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US11/612,445 US8030066B2 (en) 2000-12-11 2006-12-18 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US13/219,440 US20130004996A1 (en) 2000-12-11 2011-08-26 Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US15/060,536 US20160251664A1 (en) 2000-08-21 2016-03-03 Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38561302P 2002-06-05 2002-06-05
US60/385,613 2002-06-05

Publications (2)

Publication Number Publication Date
WO2003103600A2 WO2003103600A2 (en) 2003-12-18
WO2003103600A3 true WO2003103600A3 (en) 2004-10-07

Family

ID=29736108

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018036 WO2003103600A2 (en) 2000-08-21 2003-06-05 Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites

Country Status (3)

Country Link
EP (1) EP1539998A4 (en)
AU (1) AU2003273995A1 (en)
WO (1) WO2003103600A2 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7223576B2 (en) 1995-06-07 2007-05-29 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7282326B2 (en) 1995-06-07 2007-10-16 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US7408049B2 (en) 1997-10-24 2008-08-05 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US8883988B2 (en) 1999-03-02 2014-11-11 Life Technologies Corporation Compositions for use in recombinational cloning of nucleic acids
US8993745B2 (en) 2000-12-01 2015-03-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US9534252B2 (en) 2003-12-01 2017-01-03 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2028278B1 (en) 2000-03-30 2014-03-19 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
AU2003261449A1 (en) 2002-08-07 2004-02-25 Compositions for rna interference and methods of use thereof
EP1629096A1 (en) * 2004-05-10 2006-03-01 BASF Plant Science GmbH Methods for assembling multiple expression constructs
US7622252B2 (en) 2005-06-10 2009-11-24 Baylor College Of Medicine Generation of minicircle DNA with physiological supercoiling
CA2777835A1 (en) 2009-10-16 2011-04-21 Baylor College Of Medicine Supercoiled minicircle dna for gene therapy applications
FR3028527A1 (en) 2014-11-13 2016-05-20 Pivert IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016594A2 (en) * 2000-08-21 2002-02-28 Invitrogen Corporation Methods and reagents for molecular cloning
US20030220249A1 (en) * 2002-02-07 2003-11-27 Hackett Perry B. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation, and methods of use thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888732A (en) * 1995-06-07 1999-03-30 Life Technologies, Inc. Recombinational cloning using engineered recombination sites

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016594A2 (en) * 2000-08-21 2002-02-28 Invitrogen Corporation Methods and reagents for molecular cloning
US20030220249A1 (en) * 2002-02-07 2003-11-27 Hackett Perry B. Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation, and methods of use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1539998A4 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223576B2 (en) 1995-06-07 2007-05-29 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7282326B2 (en) 1995-06-07 2007-10-16 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7304130B2 (en) 1995-06-07 2007-12-04 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7408049B2 (en) 1997-10-24 2008-08-05 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US8883988B2 (en) 1999-03-02 2014-11-11 Life Technologies Corporation Compositions for use in recombinational cloning of nucleic acids
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US9309520B2 (en) 2000-08-21 2016-04-12 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8993745B2 (en) 2000-12-01 2015-03-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US8945884B2 (en) 2000-12-11 2015-02-03 Life Technologies Corporation Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US9534252B2 (en) 2003-12-01 2017-01-03 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same

Also Published As

Publication number Publication date
AU2003273995A8 (en) 2003-12-22
EP1539998A2 (en) 2005-06-15
EP1539998A4 (en) 2007-10-31
WO2003103600A2 (en) 2003-12-18
AU2003273995A1 (en) 2003-12-22

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