EP1539998A4 - Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites - Google Patents
Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sitesInfo
- Publication number
- EP1539998A4 EP1539998A4 EP03741892A EP03741892A EP1539998A4 EP 1539998 A4 EP1539998 A4 EP 1539998A4 EP 03741892 A EP03741892 A EP 03741892A EP 03741892 A EP03741892 A EP 03741892A EP 1539998 A4 EP1539998 A4 EP 1539998A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- synthesis
- compositions
- methods
- nucleic acid
- acid molecules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38561302P | 2002-06-05 | 2002-06-05 | |
US385613P | 2002-06-05 | ||
PCT/US2003/018036 WO2003103600A2 (en) | 2002-06-05 | 2003-06-05 | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1539998A2 EP1539998A2 (en) | 2005-06-15 |
EP1539998A4 true EP1539998A4 (en) | 2007-10-31 |
Family
ID=29736108
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03741892A Withdrawn EP1539998A4 (en) | 2002-06-05 | 2003-06-05 | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1539998A4 (en) |
AU (1) | AU2003273995A1 (en) |
WO (1) | WO2003103600A2 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7670823B1 (en) | 1999-03-02 | 2010-03-02 | Life Technologies Corp. | Compositions for use in recombinational cloning of nucleic acids |
JP4303418B2 (en) | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | Recombination cloning using nucleic acids with recombination sites |
US20020086356A1 (en) | 2000-03-30 | 2002-07-04 | Whitehead Institute For Biomedical Research | RNA sequence-specific mediators of RNA interference |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
PL218876B1 (en) | 2000-12-01 | 2015-02-27 | Europaisches Lab Für Molekularbiologie Embl | Rna interference mediating small rna molecules |
WO2004014933A1 (en) | 2002-08-07 | 2004-02-19 | University Of Massachusetts | Compositions for rna interference and methods of use thereof |
US8304189B2 (en) | 2003-12-01 | 2012-11-06 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
BRPI0511046A (en) * | 2004-05-10 | 2007-11-27 | Basf Plant Science Gmbh | methods for producing a multiple expression construct, for producing food or food, pharmaceuticals or chemicals, and a transgenic plant or plant cell, multiple expression construct, microorganism, and, use of a microorganism, a plant cell , a plant tissue, a plant organ, or a part of a plant |
US7622252B2 (en) | 2005-06-10 | 2009-11-24 | Baylor College Of Medicine | Generation of minicircle DNA with physiological supercoiling |
EP2488641B1 (en) | 2009-10-16 | 2019-01-23 | Baylor College Of Medicine | Supercoiled minicircle dna for gene therapy applications |
FR3028527A1 (en) | 2014-11-13 | 2016-05-20 | Pivert | IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5888732A (en) * | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU8835601A (en) * | 2000-08-21 | 2002-03-04 | Invitrogen Corp | Methods and reagents for molecular cloning |
AU2003209054A1 (en) * | 2002-02-07 | 2003-09-02 | Discovery Genomics, Inc. | Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation and methods of use thereof |
-
2003
- 2003-06-05 AU AU2003273995A patent/AU2003273995A1/en not_active Abandoned
- 2003-06-05 WO PCT/US2003/018036 patent/WO2003103600A2/en not_active Application Discontinuation
- 2003-06-05 EP EP03741892A patent/EP1539998A4/en not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5888732A (en) * | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
Non-Patent Citations (2)
Title |
---|
SHUMAN STEWART: "Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 269, no. 51, 23 December 1994 (1994-12-23), pages 32678 - 32684, XP002164562, ISSN: 0021-9258 * |
ZECHIEDRICH E LYNN ET AL: "Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli", GENES AND DEVELOPMENT, vol. 11, no. 19, 1997, pages 2580 - 2592, XP002450742, ISSN: 0890-9369 * |
Also Published As
Publication number | Publication date |
---|---|
AU2003273995A1 (en) | 2003-12-22 |
WO2003103600A3 (en) | 2004-10-07 |
AU2003273995A8 (en) | 2003-12-22 |
EP1539998A2 (en) | 2005-06-15 |
WO2003103600A2 (en) | 2003-12-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2002318386A1 (en) | Methods for characterization of nucleic acid molecules | |
AU2003228627A1 (en) | Application of cantilevers in nucleic acid sequencing | |
EP1062360A4 (en) | Compositions and methods for enhanced synthesis of nucleic acid molecules | |
AU2003287299A8 (en) | Process for concentration of macromolecules | |
IL174486A0 (en) | Method for small volume nucleic acid synthesis | |
EP1451344A4 (en) | Compositions and methods for use in isolation of nucleic acid molecules | |
AU2002365811A1 (en) | Methods for the selection and cloning of nucleic acid molecules free of unwanted nucleotide sequence alterations | |
AU2003270310A8 (en) | Compositions and methods for synthesizing nucleic acids | |
IL173107A0 (en) | Compositions and methods for purifying and crystallizing molecules of interest | |
AU2003230957A8 (en) | Quantitation of biological molecules | |
GB2406169B (en) | Methods and compositions relating to labeled rna molecules that reduce gene expression | |
HK1086816A1 (en) | Novel method for the synthesis of s-indoline-2-carboxylic acid and application thereof in the synthesis of perindopril | |
AU2003260291A8 (en) | Proximity-aided synthesis of templated molecules | |
AU2003273995A8 (en) | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites | |
AU2003303588A1 (en) | Methods and compositions for sequencing nucleic acid molecules | |
AU2003250506A8 (en) | Method for the characteristation of nucleic acid molecules | |
EP1660630A4 (en) | Nucleic acid biomaterials and methods of formation and use | |
AU2003211867A1 (en) | Novel method of highly sensitive nucleic acid analysis | |
AU2003268478A1 (en) | Cardiolipin molecules and method of synthesis | |
HUE039866T2 (en) | Further novel forms of interfering rna molecules | |
GB0320415D0 (en) | Analysis of macromolecules | |
GB0212853D0 (en) | Separation of molecules | |
EP1614750A4 (en) | Novel functional peptide nucleic acid and process for producing the same | |
GB2392746B (en) | Comparison of molecules using field points | |
EP1581647A3 (en) | Rapid direct sequence analysis of multi-exon genes |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20050105 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR |
|
AX | Request for extension of the european patent |
Extension state: AL LT LV MK |
|
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1079551 Country of ref document: HK |
|
RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: TEMPLE, GARY, F. Inventor name: BYRD, DEVON, R.N. Inventor name: HARTLEY, JAMES, L. Inventor name: CHEO, DAVID Inventor name: BRASCH, MICHAEL, A. Inventor name: FAN, JAMES Inventor name: GLEESON, MARTIN Inventor name: MADDEN, KNUT Inventor name: LEONG, LOUIS Inventor name: CARRINO, JOHN Inventor name: CHESNUT, JONATHAN, D. |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20070927 |
|
17Q | First examination report despatched |
Effective date: 20081021 |
|
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: LIFE TECHNOLOGIES CORPORATION |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20090603 |
|
R18D | Application deemed to be withdrawn (corrected) |
Effective date: 20090505 |
|
REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1079551 Country of ref document: HK |