EP1539998A4 - Methodes et compositions pour synthese de molecules d'acides nucleiques a l'aide de sites de reconnaissance multiples - Google Patents
Methodes et compositions pour synthese de molecules d'acides nucleiques a l'aide de sites de reconnaissance multiplesInfo
- Publication number
- EP1539998A4 EP1539998A4 EP03741892A EP03741892A EP1539998A4 EP 1539998 A4 EP1539998 A4 EP 1539998A4 EP 03741892 A EP03741892 A EP 03741892A EP 03741892 A EP03741892 A EP 03741892A EP 1539998 A4 EP1539998 A4 EP 1539998A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- synthesis
- compositions
- methods
- nucleic acid
- acid molecules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38561302P | 2002-06-05 | 2002-06-05 | |
US385613P | 2002-06-05 | ||
PCT/US2003/018036 WO2003103600A2 (fr) | 2002-06-05 | 2003-06-05 | Methodes et compositions pour synthese de molecules d'acides nucleiques a l'aide de sites de reconnaissance multiples |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1539998A2 EP1539998A2 (fr) | 2005-06-15 |
EP1539998A4 true EP1539998A4 (fr) | 2007-10-31 |
Family
ID=29736108
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03741892A Withdrawn EP1539998A4 (fr) | 2002-06-05 | 2003-06-05 | Methodes et compositions pour synthese de molecules d'acides nucleiques a l'aide de sites de reconnaissance multiples |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1539998A4 (fr) |
AU (1) | AU2003273995A1 (fr) |
WO (1) | WO2003103600A2 (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4303418B2 (ja) | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | 組換え部位を有する核酸を使用する組換えクローニング |
DE60042969D1 (de) | 1999-03-02 | 2009-10-29 | Life Technologies Corp | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren |
AU2001249622B2 (en) | 2000-03-30 | 2007-06-07 | Massachusetts Institute Of Technology | RNA sequence-specific mediators of RNA interference |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
HU230458B1 (hu) | 2000-12-01 | 2016-07-28 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Az RNS interferenciát közvetítő kis RNS molekulák |
WO2004014933A1 (fr) | 2002-08-07 | 2004-02-19 | University Of Massachusetts | Compositions pour l'interference de l'arn et procedes d'utilisation |
EP1697534B1 (fr) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation |
WO2005108568A1 (fr) * | 2004-05-10 | 2005-11-17 | Basf Plant Science Gmbh | Procédés d'assemblage de produits d'expression multiples |
US7622252B2 (en) | 2005-06-10 | 2009-11-24 | Baylor College Of Medicine | Generation of minicircle DNA with physiological supercoiling |
WO2011047318A2 (fr) | 2009-10-16 | 2011-04-21 | Baylor College Of Medicine | Adn surenroulé en minicercle pour applications en thérapie génique |
FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5888732A (en) * | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002016594A2 (fr) * | 2000-08-21 | 2002-02-28 | Invitrogen Corporation | Procedes et reactifs de clonage moleculaire |
US20030220249A1 (en) * | 2002-02-07 | 2003-11-27 | Hackett Perry B. | Factors for angiogenesis, vasculogenesis, cartilage formation, bone formation, and methods of use thereof |
-
2003
- 2003-06-05 WO PCT/US2003/018036 patent/WO2003103600A2/fr not_active Application Discontinuation
- 2003-06-05 EP EP03741892A patent/EP1539998A4/fr not_active Withdrawn
- 2003-06-05 AU AU2003273995A patent/AU2003273995A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5888732A (en) * | 1995-06-07 | 1999-03-30 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
Non-Patent Citations (2)
Title |
---|
SHUMAN STEWART: "Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 269, no. 51, 23 December 1994 (1994-12-23), pages 32678 - 32684, XP002164562, ISSN: 0021-9258 * |
ZECHIEDRICH E LYNN ET AL: "Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli", GENES AND DEVELOPMENT, vol. 11, no. 19, 1997, pages 2580 - 2592, XP002450742, ISSN: 0890-9369 * |
Also Published As
Publication number | Publication date |
---|---|
AU2003273995A8 (en) | 2003-12-22 |
WO2003103600A2 (fr) | 2003-12-18 |
AU2003273995A1 (en) | 2003-12-22 |
WO2003103600A3 (fr) | 2004-10-07 |
EP1539998A2 (fr) | 2005-06-15 |
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Legal Events
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RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: TEMPLE, GARY, F. Inventor name: BYRD, DEVON, R.N. Inventor name: HARTLEY, JAMES, L. Inventor name: CHEO, DAVID Inventor name: BRASCH, MICHAEL, A. Inventor name: FAN, JAMES Inventor name: GLEESON, MARTIN Inventor name: MADDEN, KNUT Inventor name: LEONG, LOUIS Inventor name: CARRINO, JOHN Inventor name: CHESNUT, JONATHAN, D. |
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A4 | Supplementary search report drawn up and despatched |
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RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: LIFE TECHNOLOGIES CORPORATION |
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