DE69932446D1 - Verfahren zur herstellung von nukleinsäure- und polypeptidbanken durch in vivo rekombination und ihre verwendungen - Google Patents

Verfahren zur herstellung von nukleinsäure- und polypeptidbanken durch in vivo rekombination und ihre verwendungen

Info

Publication number
DE69932446D1
DE69932446D1 DE69932446T DE69932446T DE69932446D1 DE 69932446 D1 DE69932446 D1 DE 69932446D1 DE 69932446 T DE69932446 T DE 69932446T DE 69932446 T DE69932446 T DE 69932446T DE 69932446 D1 DE69932446 D1 DE 69932446D1
Authority
DE
Germany
Prior art keywords
nucleic acid
recombination
polypeptide
methods
replication
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69932446T
Other languages
English (en)
Other versions
DE69932446T2 (de
Inventor
Raymon Bradbury
Daniele Sblattero
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scuola Internazionale Superiore di Studi Avanzati SISSA
Original Assignee
Scuola Internazionale Superiore di Studi Avanzati SISSA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scuola Internazionale Superiore di Studi Avanzati SISSA filed Critical Scuola Internazionale Superiore di Studi Avanzati SISSA
Application granted granted Critical
Publication of DE69932446D1 publication Critical patent/DE69932446D1/de
Publication of DE69932446T2 publication Critical patent/DE69932446T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Ceramic Products (AREA)
  • Medicines Containing Plant Substances (AREA)
DE69932446T 1998-11-19 1999-11-18 Verfahren zur herstellung von nukleinsäure- und polypeptidbanken durch in vivo rekombination und ihre verwendungen Expired - Lifetime DE69932446T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ITMI982509 1998-11-19
IT1998MI002509A IT1303776B1 (it) 1998-11-19 1998-11-19 Processo per la preparazione di genoteche, di polipeptidi utilizzandodette genoteche e i polipepti ottenuti.
PCT/EP1999/008856 WO2000031246A2 (en) 1998-11-19 1999-11-18 Methods for the preparation by in vivo recombination of nucleic acid and polypeptide libraries and uses thereof

Publications (2)

Publication Number Publication Date
DE69932446D1 true DE69932446D1 (de) 2006-08-31
DE69932446T2 DE69932446T2 (de) 2007-01-04

Family

ID=11381090

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69932446T Expired - Lifetime DE69932446T2 (de) 1998-11-19 1999-11-18 Verfahren zur herstellung von nukleinsäure- und polypeptidbanken durch in vivo rekombination und ihre verwendungen

Country Status (9)

Country Link
EP (1) EP1131421B1 (de)
AT (1) ATE333502T1 (de)
AU (1) AU3790300A (de)
CA (1) CA2350779A1 (de)
DE (1) DE69932446T2 (de)
ES (1) ES2268907T3 (de)
IL (2) IL143123A0 (de)
IT (1) IT1303776B1 (de)
WO (1) WO2000031246A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780620B1 (en) 1998-12-23 2004-08-24 Bristol-Myers Squibb Company Microbial transformation method for the preparation of an epothilone
US6686154B2 (en) * 2000-06-05 2004-02-03 Zyomyx, Inc. Screening of phage displayed peptides without clearing of the cell culture
AU2002366039A1 (en) * 2001-11-22 2003-06-10 Keio University Artificial antibody library with super-repertory
JP2013533211A (ja) 2010-05-04 2013-08-22 メリマック ファーマシューティカルズ インコーポレーティッド 上皮成長因子受容体（ｅｇｆｒ）に対する抗体およびその使用
US8691231B2 (en) 2011-06-03 2014-04-08 Merrimack Pharmaceuticals, Inc. Methods of treatment of tumors expressing predominantly high affinity EGFR ligands or tumors expressing predominantly low affinity EGFR ligands with monoclonal and oligoclonal anti-EGFR antibodies
US9399672B2 (en) 2012-05-17 2016-07-26 The United States Of America, As Represented By The Secretary Department Of Health And Human Services Hepatitis C virus neutralizing antibody
WO2014065822A1 (en) 2012-10-26 2014-05-01 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Hepatitis c virus neutralizing epitopes, antibodies, and methods
US20180244785A1 (en) 2017-01-09 2018-08-30 Merrimack Pharmaceuticals, Inc. Anti-fgfr antibodies and methods of use
TWI788340B (zh) 2017-04-07 2023-01-01 美商必治妥美雅史谷比公司 抗icos促效劑抗體及其用途
US11230601B2 (en) 2017-10-10 2022-01-25 Tilos Therapeutics, Inc. Methods of using anti-lap antibodies
EP3853252A1 (de) 2018-09-18 2021-07-28 Merrimack Pharmaceuticals, Inc. Anti-tnfr2-antikörper und verwendungen davon
EP3863722A2 (de) 2018-10-10 2021-08-18 Tilos Theapeutics, Inc. Anti-lap-antikörper und verwendungen davon
JP7208380B2 (ja) * 2018-10-26 2023-01-18 エフ．ホフマン－ラ ロシュ アーゲー リコンビナーゼ媒介性カセット交換を使用した多重特異性抗体スクリーニング法
CN113874083A (zh) 2019-03-01 2021-12-31 梅里麦克制药股份有限公司 抗-tnfr2抗体及其用途
WO2021207449A1 (en) 2020-04-09 2021-10-14 Merck Sharp & Dohme Corp. Affinity matured anti-lap antibodies and uses thereof
CN117430696A (zh) * 2022-07-22 2024-01-23 北京智仁美博生物科技有限公司 用于表达Fab片段库的重组系统

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5728557A (en) * 1995-06-01 1998-03-17 Merck & Co., Inc. Method of making herpes simplex type 1 mutants and mutants so produced
EP0948615B1 (de) * 1996-12-20 2004-12-15 Novozymes A/S In vivo rekombination

Also Published As

Publication number Publication date
ES2268907T3 (es) 2007-03-16
DE69932446T2 (de) 2007-01-04
ATE333502T1 (de) 2006-08-15
EP1131421B1 (de) 2006-07-19
WO2000031246A3 (en) 2000-08-17
WO2000031246A2 (en) 2000-06-02
CA2350779A1 (en) 2000-06-02
IL143123A0 (en) 2002-04-21
IT1303776B1 (it) 2001-02-23
ITMI982509A1 (it) 2000-05-19
EP1131421A2 (de) 2001-09-12
AU3790300A (en) 2000-06-13
IL143123A (en) 2009-11-18

Similar Documents

Publication Publication Date Title
DE69932446D1 (de) Verfahren zur herstellung von nukleinsäure- und polypeptidbanken durch in vivo rekombination und ihre verwendungen
Morris et al. Fast atom bombardment: a new mass spectrometric method for peptide sequence analysis
ATE460472T1 (de) Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen
ATE294229T1 (de) Verfahren zur trocknung von kompetenten zellen
ATE204298T1 (de) Hybrider mensch/tier faktor viii
DE60038572D1 (de) Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
DK0586618T3 (da) Anvendelse af nuklainsyreanaloger i diagnostiske og analytiske fremgangsmåder
FI925986A0 (fi) Slumpartat bio-oligomer bibliotek, ett foerfarande foer syntetisering avdet samt ett foerfarande foer anvaendning av det
ATE449850T1 (de) Mutanten von flt3-l und ihre verwendungen
DE59708838D1 (de) Spiegelselektion und spiegelevolution von nucleinsäuren
DE69738905D1 (de) Methoden und zusammensetzungen zur transformation von zellen
ATE425175T1 (de) Verfahren und zusammensetzungen zur bestimmung von sequenzen von nukleinsäure-molekülen
AU699568B2 (en) A method for identification of biologically active peptides and nucleic acids
CA2413156A1 (en) Expression vectors
DE69821376D1 (de) Verfahren zur proteinrückfaltung durch verwendung zwitterionischer wirkstoffe von niedrigem molekulargewicht
CA2257115A1 (en) Spruce budworm antifreeze proteins, genes and methods of using same
ID27206A (id) Generasi produk-produk perbanyakan gena dari protein-protein fusi simerik melalui pemutusan endoprotease ubikuitus
DK1098991T3 (da) Hidtil ukendte fremgangsmåder til identifikation af ligand- og målbiomolekyler
DE60036794D1 (de) Proteomabbau
Lempens et al. Noncovalent synthesis of protein dendrimers
DE60233347D1 (de) Schnellreifende fluoreszierende proteine und verfahren zu deren anwendung
Fox Thermodynamic perspectives and the origin of life
WO1998042868A3 (en) Screening method for proline-rich proteins
Hoffmann et al. Artificial proteins screened from combinatorial chemical libraries with strong binding to double-stranded oligodeoxyribonucleotides: towards repressors to long terminal repeat controlled viral transcription
ATE319821T1 (de) Verfahren und produkt zur regulation der reaktionsempfindlichkeit von zellen auf externe signale

Legal Events

Date Code Title Description
8364 No opposition during term of opposition