WO1998042868A3 - Screening method for proline-rich proteins - Google Patents

Screening method for proline-rich proteins Download PDF

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Publication number
WO1998042868A3
WO1998042868A3 PCT/IB1998/000525 IB9800525W WO9842868A3 WO 1998042868 A3 WO1998042868 A3 WO 1998042868A3 IB 9800525 W IB9800525 W IB 9800525W WO 9842868 A3 WO9842868 A3 WO 9842868A3
Authority
WO
WIPO (PCT)
Prior art keywords
proline
rich proteins
screening method
amino acid
screening
Prior art date
Application number
PCT/IB1998/000525
Other languages
French (fr)
Other versions
WO1998042868A2 (en
Inventor
Vega Masignani
Guido Grandi
Rino Rappuoli
Beatrice Arico
Original Assignee
Chiron Spa
Vega Masignani
Guido Grandi
Rino Rappuoli
Beatrice Arico
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Spa, Vega Masignani, Guido Grandi, Rino Rappuoli, Beatrice Arico filed Critical Chiron Spa
Priority to JP54534198A priority Critical patent/JP2001514527A/en
Priority to CA002285480A priority patent/CA2285480A1/en
Priority to EP98910914A priority patent/EP0973942A2/en
Publication of WO1998042868A2 publication Critical patent/WO1998042868A2/en
Publication of WO1998042868A3 publication Critical patent/WO1998042868A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

Abstract

A method for screening a pathogenic microorganism for a secreted or cell-surface protein potentially useful as a vaccine antigen involves the step of identifying proline-rich proteins, either as the amino acid level, or preferably at the DNA level by employing PCR. Suitable consensus sequences are described as (PXm)nP or (PPXq)nPP wherein: P is proline; X is any amino acid, m is from 1 to 5; q is 1 or 2; and n is 2 or more.
PCT/IB1998/000525 1997-03-24 1998-03-24 Screening method for proline-rich proteins WO1998042868A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP54534198A JP2001514527A (en) 1997-03-24 1998-03-24 Screening methods for proline-rich proteins
CA002285480A CA2285480A1 (en) 1997-03-24 1998-03-24 Screening method for proline-rich proteins
EP98910914A EP0973942A2 (en) 1997-03-24 1998-03-24 Screening method for proline-rich proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9706078.4 1997-03-24
GBGB9706078.4A GB9706078D0 (en) 1997-03-24 1997-03-24 Screening method and proteins and dna identified thereby

Publications (2)

Publication Number Publication Date
WO1998042868A2 WO1998042868A2 (en) 1998-10-01
WO1998042868A3 true WO1998042868A3 (en) 1998-12-23

Family

ID=10809774

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1998/000525 WO1998042868A2 (en) 1997-03-24 1998-03-24 Screening method for proline-rich proteins

Country Status (5)

Country Link
EP (1) EP0973942A2 (en)
JP (1) JP2001514527A (en)
CA (1) CA2285480A1 (en)
GB (1) GB9706078D0 (en)
WO (1) WO1998042868A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066731A2 (en) * 2011-10-31 2013-05-10 Merck Sharp & Dohme Corp. Protective vaccine based on staphylococcus aureus sa2451 protein

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992006191A1 (en) * 1990-09-28 1992-04-16 Protein Engineering Corporation Proteinaceous anti-dental plaque agents
WO1995031216A1 (en) * 1994-05-18 1995-11-23 Medical Research Council Vaccine against mycobacterial infections
US5476929A (en) * 1991-02-15 1995-12-19 Uab Research Foundation Structural gene of pneumococcal protein
US5609876A (en) * 1993-08-09 1997-03-11 University Of Pittsburgh Of The Commonwealth System Of Higher Education Peptide vaccines and associated methods for protection against feline leukemia virus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992006191A1 (en) * 1990-09-28 1992-04-16 Protein Engineering Corporation Proteinaceous anti-dental plaque agents
US5476929A (en) * 1991-02-15 1995-12-19 Uab Research Foundation Structural gene of pneumococcal protein
US5609876A (en) * 1993-08-09 1997-03-11 University Of Pittsburgh Of The Commonwealth System Of Higher Education Peptide vaccines and associated methods for protection against feline leukemia virus
WO1995031216A1 (en) * 1994-05-18 1995-11-23 Medical Research Council Vaccine against mycobacterial infections

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CLEAVINGER C M ET AL.: "Identification of mycoplasma membrane proteins by systematic Tn phoA mutagenesis of a recombinant library", MOLECULAR MICROBIOLOGY, vol. 18, no. 2, 1995, pages 283 - 293, XP002072133 *
DALLO S F ET AL.: "Characterization of the gene for a 30-kilodalton adhesin-related protein of Mycoplasma pneumoniae", INFECTION AND IMMUNITY, vol. 58, no. 12, 1990, pages 4163 - 4165, XP002072130 *
MARGOLIN W ET AL.: "Cloning and characterization of a Rhizobium meliloti homolog of the Escherichia coli cell division gene ftsZ", JOURNAL OF BACTERIOLOGY, vol. 173, no. 18, 1991, pages 5822 - 5830, XP002072129 *
NONET M L ET AL.: "The hisT-purF region of the Eschirichia coli K-12 chromosome", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 262, no. 25, 1987, pages 12209 - 12217, XP002072131 *
TASCON R E ET AL.: "Vaccination against tuberculosis by DNA injection", NATURE MEDICINE, vol. 2, no. 8, 1996, pages 888 - 892, XP002080167 *
WANG M AND COHEN S N: "ard-1 A human gene that reverses the effects of temperature-sensitive and deletion mutants in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages", PRCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 91, 1994, pages 10591 - 10595, XP002072132 *
WILLIAMSON S ET AL.: "Theileria annulata sporozoite surface antigen expressed in Escherichia coli elicits neutralizing antibody", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 86, 1989, pages 4639 - 4643, XP002080166 *

Also Published As

Publication number Publication date
GB9706078D0 (en) 1997-05-14
CA2285480A1 (en) 1998-10-01
EP0973942A2 (en) 2000-01-26
JP2001514527A (en) 2001-09-11
WO1998042868A2 (en) 1998-10-01

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