CA1312566C - Method for increasing the expression of polypeptides in recombinant cell culture - Google Patents

Method for increasing the expression of polypeptides in recombinant cell culture

Info

Publication number
CA1312566C
CA1312566C CA577153A CA577153A CA1312566C CA 1312566 C CA1312566 C CA 1312566C CA 577153 A CA577153 A CA 577153A CA 577153 A CA577153 A CA 577153A CA 1312566 C CA1312566 C CA 1312566C
Authority
CA
Canada
Prior art keywords
expression
polypeptides
increasing
cell culture
recombinant cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA577153A
Inventor
Arthur D Levinson
Jennie P Mather
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to CA000616540A priority Critical patent/CA1333778C/en
Application granted granted Critical
Publication of CA1312566C publication Critical patent/CA1312566C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Abstract

Viable cell proportions and total cell densities in recombinant eukaryotic cell cultures are increased by transfecting a eukaryotic host cell with nucleic acid encoding a desired polypeptide and with an oncogene. Transfectant cultures are screened thereafter for the expression of elevated quantities of the desired polypeptide as compared to expression from the parent host cell.
CA577153A 1987-09-11 1988-09-12 Method for increasing the expression of polypeptides in recombinant cell culture Expired - Lifetime CA1312566C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA000616540A CA1333778C (en) 1987-09-11 1993-01-05 Method for increasing the expression of polypeptides in recombinant cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9724687A 1987-09-11 1987-09-11

Publications (1)

Publication Number Publication Date
CA1312566C true CA1312566C (en) 1993-01-12

Family

ID=22262433

Family Applications (1)

Application Number Title Priority Date Filing Date
CA577153A Expired - Lifetime CA1312566C (en) 1987-09-11 1988-09-12 Method for increasing the expression of polypeptides in recombinant cell culture

Country Status (8)

Country Link
EP (1) EP0307248B1 (en)
JP (1) JP2796547B2 (en)
AT (1) ATE113984T1 (en)
AU (1) AU612404B2 (en)
CA (1) CA1312566C (en)
DE (1) DE3852082T2 (en)
ES (1) ES2065915T3 (en)
IE (1) IE64979B1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5256553A (en) * 1987-10-09 1993-10-26 Immunex Corporation Multiple promoter transforming retroviral vectors
US5648254A (en) * 1988-01-15 1997-07-15 Zymogenetics, Inc. Co-expression in eukaryotic cells
CA2005311C (en) * 1988-12-13 2006-05-30 Edward A. Hoover Prototype felv isolates for use in disease models and vaccines
FR2650838A1 (en) * 1989-08-09 1991-02-15 Transgene Sa FACTOR IX EXPRESSION VECTORS IN A SUPERIOR EUKARYOTE CELL, PROCESS FOR THE PREPARATION OF FACTOR IX BY TRANSGENIC ANIMALS, AND FACTOR IX OBTAINED
GB8928884D0 (en) * 1989-12-21 1990-02-28 Feldmann Marc Transformed cell lines
WO1991014781A1 (en) * 1990-03-19 1991-10-03 Henkel Research Corporation METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
AUPN442295A0 (en) * 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT85828B (en) * 1986-09-30 1990-08-31 Smithkline Beecham Corp PROFFESS OF PRODUCTION OF A LINE OF MAMIFERO, RECOMBINANT, CELL LINE THUS OBTAINED AND PROCESS OF PREPARATION OF A GENE
EP0263908B1 (en) * 1986-10-15 1991-09-25 Battelle Memorial Institute Method for the production of proteins by means of inducible expression systems in genetically modified eukaryotic host-cells multiplicated in-vivo

Also Published As

Publication number Publication date
EP0307248A2 (en) 1989-03-15
ES2065915T3 (en) 1995-03-01
DE3852082T2 (en) 1995-05-04
EP0307248B1 (en) 1994-11-09
ATE113984T1 (en) 1994-11-15
IE64979B1 (en) 1995-09-20
JPH01120300A (en) 1989-05-12
JP2796547B2 (en) 1998-09-10
AU2209988A (en) 1989-04-20
AU612404B2 (en) 1991-07-11
IE882769L (en) 1989-03-11
EP0307248A3 (en) 1990-04-25
DE3852082D1 (en) 1994-12-15

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