ES8205432A1 - Polypeptide production - Google Patents

Polypeptide production

Info

Publication number
ES8205432A1
ES8205432A1 ES500397A ES500397A ES8205432A1 ES 8205432 A1 ES8205432 A1 ES 8205432A1 ES 500397 A ES500397 A ES 500397A ES 500397 A ES500397 A ES 500397A ES 8205432 A1 ES8205432 A1 ES 8205432A1
Authority
ES
Spain
Prior art keywords
gene
fused
polypeptide
eukaryotic
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES500397A
Other languages
Spanish (es)
Other versions
ES500397A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of ES8205432A1 publication Critical patent/ES8205432A1/en
Publication of ES500397A0 publication Critical patent/ES500397A0/en
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Compositions and methods featuring, in one aspect, a method of providing a fused gene having a promoter located an optimal distance from the translation start site, the method including providing a fused gene having a region of a gene coding for a eukaryotic or prokaryotic polypeptide fused to a region of a gene coding for an assayable polypeptide, inserting a portable promotor at varying distances in front of the translation start site, cloning the fused genes into microorganisms, selecting those producing the greatest amount of assayable polypeptide, and reconstituting the gene for the eukaryotic or prokaryotic protein.
ES500397A 1980-03-17 1981-03-16 A METHOD OF DETERMINING THE AMOUNT OF AN ENCAHRIIOTIC OR PROCARIOTIC POLYPEPTIDE PRODUCED BY A MICROORGANISM Granted ES500397A0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13115280A 1980-03-17 1980-03-17

Publications (2)

Publication Number Publication Date
ES8205432A1 true ES8205432A1 (en) 1982-06-01
ES500397A0 ES500397A0 (en) 1982-06-01

Family

ID=22448125

Family Applications (2)

Application Number Title Priority Date Filing Date
ES500397A Granted ES500397A0 (en) 1980-03-17 1981-03-16 A METHOD OF DETERMINING THE AMOUNT OF AN ENCAHRIIOTIC OR PROCARIOTIC POLYPEPTIDE PRODUCED BY A MICROORGANISM
ES510007A Granted ES510007A0 (en) 1980-03-17 1982-03-01 A METHOD OF PROVIDING A SAMPLE OF A MERGED GENE CODING A DESIRED EUCARYTIC OR PROCARIOTIC POLYPEPTIDE.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES510007A Granted ES510007A0 (en) 1980-03-17 1982-03-01 A METHOD OF PROVIDING A SAMPLE OF A MERGED GENE CODING A DESIRED EUCARYTIC OR PROCARIOTIC POLYPEPTIDE.

Country Status (11)

Country Link
JP (1) JPS56167698A (en)
AT (1) AT378004B (en)
BE (1) BE887991A (en)
CA (1) CA1198385A (en)
DE (2) DE3110031A1 (en)
ES (2) ES500397A0 (en)
FR (1) FR2478125B1 (en)
GB (1) GB2071671B (en)
IT (1) IT1144705B (en)
NL (1) NL8101289A (en)
SE (1) SE8101665L (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4874702A (en) * 1980-09-08 1989-10-17 Biogen, Inc. Vectors and methods for making such vectors and for expressive cloned genes
FI64813C (en) * 1980-12-31 1984-01-10 Ilkka Antero Palva FOERFARANDE FOER PRODUCERING AV ETT UTVALT AEGGVITEAEMNE OCH VID FOERFARANDET ANVAENDA REKOMBINANTPLASMIDVEKTORER
IL66263A (en) * 1981-08-10 1985-12-31 Genex Corp Expression vectors for introducing a gene into a procaryotic organism
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0109428B1 (en) * 1982-05-25 1988-04-06 Brandeis University Cloning vector with indicator genes which have been inactivated by frameshift
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
JPS5939899A (en) * 1982-08-27 1984-03-05 Gakuzo Tamura Novel vector
DE3484078D1 (en) * 1983-06-27 1991-03-14 Genentech Inc TRANSFERABLE INDUCIBLE CONTROL SYSTEMS, THESE EXPRESSION VECTORS, WITH THESE TRANSFORMED MICROORGANISMS AND THEIR USE IN EXPRESSING EXOGENIC PROTEIN.
EP0172194B1 (en) * 1984-02-08 1994-01-19 Cetus Oncology Corporation Control systems for recombinant manipulations
US4923808A (en) * 1985-03-12 1990-05-08 Genentech, Inc. Method for identifying mutants secreting high levels of heterologous proteins
IL78775A (en) * 1985-05-15 1992-06-21 Biotech Australia Pty Ltd Oral vaccines
US5030576A (en) * 1986-04-30 1991-07-09 Genentech, Inc. Receptors for efficient determination of ligands and their antagonists or agonists
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
WO2016208385A1 (en) 2015-06-23 2016-12-29 富士フイルム株式会社 Electroacoustic conversion film and electroacoustic transducer

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT373280B (en) * 1977-11-08 1984-01-10 Genentech Inc METHOD FOR GENERATING A SPECIFIC POLYPEPTIDE FROM A MICROBIAL RECOMBINATION CLONING SUPPORT
FI792481A (en) * 1978-08-11 1980-02-12 Univ California SYNTHESIS OF ENCAROYTIC PROTEIN GENOM ANVAENDNING AV MICRO-ORGANISM
NL8000127A (en) * 1979-01-15 1980-07-17 Harvard College METHOD FOR FORMING PROKARYOTIC OR EUKARYOTIC PROTEINS AND A GENERALLY COUPLED GENE.

Also Published As

Publication number Publication date
BE887991A (en) 1981-07-16
FR2478125B1 (en) 1985-07-05
GB2071671B (en) 1983-10-05
FR2478125A1 (en) 1981-09-18
CA1198385A (en) 1985-12-24
GB2071671A (en) 1981-09-23
IT8167359A0 (en) 1981-03-16
NL8101289A (en) 1981-10-16
ES8306177A1 (en) 1983-05-01
DE3153267A1 (en) 1985-08-14
ES510007A0 (en) 1983-05-01
AT378004B (en) 1985-06-10
JPS56167698A (en) 1981-12-23
ES500397A0 (en) 1982-06-01
DE3110031A1 (en) 1982-01-07
ATA122281A (en) 1984-10-15
IT1144705B (en) 1986-10-29
SE8101665L (en) 1981-09-18

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