IL84702A - Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors - Google Patents
Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectorsInfo
- Publication number
- IL84702A IL84702A IL8470287A IL8470287A IL84702A IL 84702 A IL84702 A IL 84702A IL 8470287 A IL8470287 A IL 8470287A IL 8470287 A IL8470287 A IL 8470287A IL 84702 A IL84702 A IL 84702A
- Authority
- IL
- Israel
- Prior art keywords
- host cells
- vectors
- protein
- transformed
- expression
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8415186A GB8415186D0 (en) | 1984-06-14 | 1984-06-14 | Polypeptides |
Publications (1)
Publication Number | Publication Date |
---|---|
IL84702A true IL84702A (en) | 1993-07-08 |
Family
ID=10562425
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL8470287A IL84702A (en) | 1984-06-14 | 1987-12-03 | Expression vectors for expression of a protein in higher eukaryotic host cells comprising an enhancer/promoter unit, a protein encoding sequence, signal sequences, and selection marker gene, host cells transformed thereby, a method for producing the protein using the transformed host cells and host cells transformed by the vectors for cloning and multiplication of the vectors |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0182838A1 (en) |
JP (1) | JPS61502377A (en) |
DK (1) | DK70486D0 (en) |
GB (1) | GB8415186D0 (en) |
IL (1) | IL84702A (en) |
WO (1) | WO1986000089A1 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8607502D0 (en) * | 1986-03-26 | 1986-04-30 | Antibioticos Sa | Penicillium chrysogenum |
US4861614A (en) * | 1988-02-19 | 1989-08-29 | General Mills, Inc. | Instant traditional oatmeal and method of preparation |
JPH03503599A (en) * | 1988-03-24 | 1991-08-15 | ザ・ジェネラル・ホスピタル・コーポレーション | artificial chromosome vector |
US5270201A (en) * | 1988-03-24 | 1993-12-14 | The General Hospital Corporation | Artificial chromosome vector |
US6004776A (en) * | 1989-07-07 | 1999-12-21 | Unilever Patent Holdings, B.V. | Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
DE69031710T2 (en) * | 1989-07-07 | 1998-03-05 | Unilever Nv | METHOD FOR PRODUCING A PROTEIN BY MEANS OF A MUSHROOM TRANSFORMED BY MULTIPLE COPY INTEGRATION OF AN EXPRESSION VECTOR |
US5674728A (en) * | 1993-11-03 | 1997-10-07 | Novartis Corporation | Aspergillus niger vacuolar aspartyl protease |
JP2001501089A (en) * | 1996-09-24 | 2001-01-30 | バハマイア,アンドレアス | DNA constructs and methods for producing proteins using these DNA constructs |
US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
US9757330B2 (en) | 2013-10-18 | 2017-09-12 | Industrial Technology Research Institute | Recipe for in-situ gel, and implant, drug delivery system formed thereby |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
AU572108B2 (en) * | 1983-01-19 | 1988-05-05 | Genentech Inc. | Human tpa production using vectors coding for dhfr protein |
-
1984
- 1984-06-14 GB GB8415186A patent/GB8415186D0/en active Pending
-
1985
- 1985-06-11 JP JP50258885A patent/JPS61502377A/en active Pending
- 1985-06-11 WO PCT/EP1985/000278 patent/WO1986000089A1/en not_active Application Discontinuation
- 1985-06-11 EP EP19850902543 patent/EP0182838A1/en not_active Withdrawn
-
1986
- 1986-02-13 DK DK70486A patent/DK70486D0/en not_active Application Discontinuation
-
1987
- 1987-12-03 IL IL8470287A patent/IL84702A/en unknown
Also Published As
Publication number | Publication date |
---|---|
DK70486A (en) | 1986-02-13 |
DK70486D0 (en) | 1986-02-13 |
WO1986000089A1 (en) | 1986-01-03 |
JPS61502377A (en) | 1986-10-23 |
GB8415186D0 (en) | 1984-07-18 |
EP0182838A1 (en) | 1986-06-04 |
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