IL197917A0 - Genetic incorporation of unnatural amino acids into proteins in mammalian cells - Google Patents

Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Info

Publication number
IL197917A0
IL197917A0 IL197917A IL19791709A IL197917A0 IL 197917 A0 IL197917 A0 IL 197917A0 IL 197917 A IL197917 A IL 197917A IL 19791709 A IL19791709 A IL 19791709A IL 197917 A0 IL197917 A0 IL 197917A0
Authority
IL
Israel
Prior art keywords
proteins
amino acids
mammalian cells
unnatural amino
genetic incorporation
Prior art date
Application number
IL197917A
Other languages
English (en)
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of IL197917A0 publication Critical patent/IL197917A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL197917A 2006-10-18 2009-04-05 Genetic incorporation of unnatural amino acids into proteins in mammalian cells IL197917A0 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85300806P 2006-10-18 2006-10-18
US92345807P 2007-04-12 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Publications (1)

Publication Number Publication Date
IL197917A0 true IL197917A0 (en) 2011-08-01

Family

ID=39512252

Family Applications (1)

Application Number Title Priority Date Filing Date
IL197917A IL197917A0 (en) 2006-10-18 2009-04-05 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Country Status (12)

Country Link
US (2) US20080166766A1 (enExample)
EP (1) EP2069481A4 (enExample)
JP (1) JP2010506591A (enExample)
KR (1) KR20090094812A (enExample)
CN (1) CN101535338A (enExample)
AU (1) AU2007333010A1 (enExample)
BR (1) BRPI0718389A2 (enExample)
CA (1) CA2665678A1 (enExample)
IL (1) IL197917A0 (enExample)
MX (1) MX2009004043A (enExample)
RU (1) RU2009112104A (enExample)
WO (1) WO2008073184A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) * 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
EP2647721B1 (en) 2010-12-03 2019-02-27 The University of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
BR112015006368B1 (pt) * 2012-09-24 2022-02-08 Medimmune Limited Processo para produção de linha celular eucariótica estável
BR112015022641B1 (pt) * 2013-03-15 2023-02-28 Medimmune Limited Construção de dna e processo de preparação de uma proteína-alvo
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN112725282A (zh) * 2016-01-27 2021-04-30 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
AU2018221227A1 (en) 2017-02-17 2019-08-15 Rubius Therapeutics, Inc. Functionalized erythroid cells
CN117286182A (zh) * 2017-06-02 2023-12-26 Ambrx公司 促进含非天然氨基酸的蛋白质产生的方法和组合物
EP3634988A4 (en) 2017-06-06 2021-02-24 Kindred Biosciences, Inc. ERYTHROPOIETIN AND SIMILAR COMPOUNDS FOR VETERINARY USE
US20210062176A1 (en) 2018-04-19 2021-03-04 Kindred Biosciences, Inc. Variant Asparaginase Polypeptides for Medical Use
US20210356377A1 (en) * 2018-09-07 2021-11-18 Medimmune, Llc Methods Of Cell Selection
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
JP2022552137A (ja) * 2019-10-15 2022-12-15 トラスティーズ オブ ボストン カレッジ 非天然アミノ酸組み込み増強のためのキメラ耐熱性アミノアシルｔＲＮＡシンテターゼ
WO2021117848A1 (ja) * 2019-12-12 2021-06-17 中外製薬株式会社 非天然アミノ酸を含むペプチドの製造方法
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697A (zh) * 2024-12-20 2025-03-21 天津大学 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
DK1578949T3 (da) 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
CA2503749A1 (en) * 2002-10-31 2004-05-13 Riken Method of expressing protein having unnatural amino acid integrated thereinto
EP1583816A4 (en) 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
EA200700469A1 (ru) * 2003-02-14 2008-10-30 Байоджен Айдек Ма Инк. Экспрессионная кассета и вектор для временной или постоянной экспрессии экзогенных молекул
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
WO2005003294A2 (en) * 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005038002A2 (en) 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
BRPI0417801A (pt) 2003-12-18 2007-04-10 Scripps Research Inst incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas
BRPI0507159A (pt) 2004-02-02 2007-06-26 Ambrx Inc polipeptìdeos em feixe de quatro hélices humanos modificados e seus usos
AU2005248392A1 (en) 2004-05-25 2005-12-08 Irm, Llc Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
EP1797177A4 (en) * 2004-09-21 2008-07-09 Scripps Research Inst ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE
EP1794323B1 (en) 2004-09-21 2014-08-06 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
JP2008513806A (ja) 2004-09-22 2008-05-01 ザ スクリップス リサーチ インスティテュート Ｎｍｒ試験のための蛋白質の部位特異的標識
EP1809758A4 (en) 2004-10-20 2009-04-01 Scripps Research Inst LOCAL IN VIVO INSTALLATION OF N-ACETYLGALACTOSAMINE AMINO ACIDS IN EU BACTERIA
AU2005330514B2 (en) 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CA2638763A1 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現

Also Published As

Publication number Publication date
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
RU2009112104A (ru) 2010-11-27
MX2009004043A (es) 2009-04-27
JP2010506591A (ja) 2010-03-04
WO2008073184A2 (en) 2008-06-19
EP2069481A4 (en) 2010-01-06
BRPI0718389A2 (pt) 2012-04-10
KR20090094812A (ko) 2009-09-08
WO2008073184A3 (en) 2008-11-27
US20100021963A1 (en) 2010-01-28
AU2007333010A1 (en) 2008-06-19
CA2665678A1 (en) 2008-06-19
EP2069481A2 (en) 2009-06-17
CN101535338A (zh) 2009-09-16

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