CA2665678A1 - Genetic incorporation of unnatural amino acids into proteins in mammalian cells - Google Patents
Genetic incorporation of unnatural amino acids into proteins in mammalian cells Download PDFInfo
- Publication number
- CA2665678A1 CA2665678A1 CA002665678A CA2665678A CA2665678A1 CA 2665678 A1 CA2665678 A1 CA 2665678A1 CA 002665678 A CA002665678 A CA 002665678A CA 2665678 A CA2665678 A CA 2665678A CA 2665678 A1 CA2665678 A1 CA 2665678A1
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- Prior art keywords
- trna
- amino acid
- unnatural amino
- host cell
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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| PCT/US2007/022232 WO2008073184A2 (en) | 2006-10-18 | 2007-10-17 | Genetic incorporation of unnatural amino acids into proteins in mammalian cells |
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| CN114134141A (zh) * | 2021-11-03 | 2022-03-04 | 浙江大学 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
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| EP1490483B1 (en) * | 2001-04-19 | 2015-06-03 | The Scripps Research Institute | In vivo incorporation of unnatural amino acids |
| KR20050073559A (ko) * | 2002-10-16 | 2005-07-14 | 더 스크립스 리서치 인스티튜트 | 당단백질 합성 |
| CN103289960B (zh) | 2003-04-17 | 2017-04-26 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
| DE602004011789T2 (de) * | 2003-07-07 | 2009-02-12 | The Scripps Research Institute, La Jolla | Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen |
| WO2010114615A2 (en) * | 2009-04-03 | 2010-10-07 | The Scripps Research Institute | A facile system for encoding unnatural amino acids in mammalian cells |
| EP2647721B1 (en) | 2010-12-03 | 2019-02-27 | The University of Tokyo | Peptide with safer secondary structure, peptide library, and production methods for same |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| BR112015006368B1 (pt) * | 2012-09-24 | 2022-02-08 | Medimmune Limited | Processo para produção de linha celular eucariótica estável |
| BR112015022641B1 (pt) * | 2013-03-15 | 2023-02-28 | Medimmune Limited | Construção de dna e processo de preparação de uma proteína-alvo |
| CN105420203B (zh) * | 2014-09-03 | 2018-11-16 | 中国科学院生物物理研究所 | 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族 |
| CN105400816A (zh) * | 2015-11-24 | 2016-03-16 | 深圳市第二人民医院 | 用于膀胱癌检测的逻辑与门基因线路的构建方法 |
| GB201600512D0 (en) | 2016-01-12 | 2016-02-24 | Univ York | Recombinant protein production |
| CN112725282A (zh) * | 2016-01-27 | 2021-04-30 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| AU2018221227A1 (en) | 2017-02-17 | 2019-08-15 | Rubius Therapeutics, Inc. | Functionalized erythroid cells |
| CN117286182A (zh) * | 2017-06-02 | 2023-12-26 | Ambrx公司 | 促进含非天然氨基酸的蛋白质产生的方法和组合物 |
| EP3634988A4 (en) | 2017-06-06 | 2021-02-24 | Kindred Biosciences, Inc. | ERYTHROPOIETIN AND SIMILAR COMPOUNDS FOR VETERINARY USE |
| US20210062176A1 (en) | 2018-04-19 | 2021-03-04 | Kindred Biosciences, Inc. | Variant Asparaginase Polypeptides for Medical Use |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| US20220325269A1 (en) * | 2019-08-08 | 2022-10-13 | Brickbio, Inc. | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
| JP2022552137A (ja) * | 2019-10-15 | 2022-12-15 | トラスティーズ オブ ボストン カレッジ | 非天然アミノ酸組み込み増強のためのキメラ耐熱性アミノアシルｔＲＮＡシンテターゼ |
| WO2021117848A1 (ja) * | 2019-12-12 | 2021-06-17 | 中外製薬株式会社 | 非天然アミノ酸を含むペプチドの製造方法 |
| CN113388654A (zh) * | 2021-06-04 | 2021-09-14 | 清华大学 | 一种基于线形模板无细胞体系非天然氨基酸嵌入方法 |
| CN119662697A (zh) * | 2024-12-20 | 2025-03-21 | 天津大学 | 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用 |
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| KR20050073559A (ko) | 2002-10-16 | 2005-07-14 | 더 스크립스 리서치 인스티튜트 | 당단백질 합성 |
| DK1578949T3 (da) | 2002-10-16 | 2009-07-27 | Scripps Research Inst | Stedspecifik optagelse af keto-aminnosyrer hos proteiner |
| CA2503749A1 (en) * | 2002-10-31 | 2004-05-13 | Riken | Method of expressing protein having unnatural amino acid integrated thereinto |
| EP1583816A4 (en) | 2002-12-22 | 2007-06-13 | Scripps Research Inst | PROTEIN ARRAYS |
| EA200700469A1 (ru) * | 2003-02-14 | 2008-10-30 | Байоджен Айдек Ма Инк. | Экспрессионная кассета и вектор для временной или постоянной экспрессии экзогенных молекул |
| CN103289960B (zh) | 2003-04-17 | 2017-04-26 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
| WO2005003294A2 (en) * | 2003-06-18 | 2005-01-13 | The Scripps Research Institute | Unnatural reactive amino acid genetic code additions |
| WO2005007870A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF |
| DE602004011789T2 (de) | 2003-07-07 | 2009-02-12 | The Scripps Research Institute, La Jolla | Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen |
| WO2005007624A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof |
| WO2005038002A2 (en) | 2003-10-14 | 2005-04-28 | The Scripps Research Institute | Site-specific incorporation of redox active amino acids into proteins |
| BRPI0417801A (pt) | 2003-12-18 | 2007-04-10 | Scripps Research Inst | incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas |
| BRPI0507159A (pt) | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos em feixe de quatro hélices humanos modificados e seus usos |
| AU2005248392A1 (en) | 2004-05-25 | 2005-12-08 | Irm, Llc | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
| EP1797177A4 (en) * | 2004-09-21 | 2008-07-09 | Scripps Research Inst | ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE |
| EP1794323B1 (en) | 2004-09-21 | 2014-08-06 | The Scripps Research Institute | In vivo incorporation of alkynyl amino acids into proteins in eubacteria |
| JP2008513806A (ja) | 2004-09-22 | 2008-05-01 | ザ スクリップス リサーチ インスティテュート | Ｎｍｒ試験のための蛋白質の部位特異的標識 |
| EP1809758A4 (en) | 2004-10-20 | 2009-04-01 | Scripps Research Inst | LOCAL IN VIVO INSTALLATION OF N-ACETYLGALACTOSAMINE AMINO ACIDS IN EU BACTERIA |
| AU2005330514B2 (en) | 2004-10-27 | 2011-05-12 | The Scripps Research Institute | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| CA2624290A1 (en) | 2005-10-12 | 2007-04-26 | Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
| CA2638763A1 (en) | 2006-03-09 | 2007-09-13 | The Scripps Research Institute | System for the expression of orthogonal translation components in eubacterial host cells |
| JP5249194B2 (ja) | 2006-03-16 | 2013-07-31 | ザ スクリプス リサーチ インスティチュート | 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現 |
-
2007
- 2007-10-17 US US11/975,351 patent/US20080166766A1/en not_active Abandoned
- 2007-10-17 WO PCT/US2007/022232 patent/WO2008073184A2/en not_active Ceased
- 2007-10-17 AU AU2007333010A patent/AU2007333010A1/en not_active Abandoned
- 2007-10-17 CN CNA2007800390157A patent/CN101535338A/zh active Pending
- 2007-10-17 RU RU2009112104/10A patent/RU2009112104A/ru not_active Application Discontinuation
- 2007-10-17 MX MX2009004043A patent/MX2009004043A/es not_active Application Discontinuation
- 2007-10-17 CA CA002665678A patent/CA2665678A1/en not_active Abandoned
- 2007-10-17 JP JP2009533372A patent/JP2010506591A/ja active Pending
- 2007-10-17 EP EP07870795A patent/EP2069481A4/en not_active Withdrawn
- 2007-10-17 KR KR1020097009346A patent/KR20090094812A/ko not_active Ceased
- 2007-10-17 US US12/311,545 patent/US9624485B2/en not_active Expired - Fee Related
- 2007-10-17 BR BRPI0718389-5A patent/BRPI0718389A2/pt not_active IP Right Cessation
-
2009
- 2009-04-05 IL IL197917A patent/IL197917A0/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114134141A (zh) * | 2021-11-03 | 2022-03-04 | 浙江大学 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
| CN114134141B (zh) * | 2021-11-03 | 2024-01-30 | 杭州嵌化合生医药科技有限公司 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20080166766A1 (en) | 2008-07-10 |
| US9624485B2 (en) | 2017-04-18 |
| RU2009112104A (ru) | 2010-11-27 |
| MX2009004043A (es) | 2009-04-27 |
| JP2010506591A (ja) | 2010-03-04 |
| WO2008073184A2 (en) | 2008-06-19 |
| EP2069481A4 (en) | 2010-01-06 |
| BRPI0718389A2 (pt) | 2012-04-10 |
| KR20090094812A (ko) | 2009-09-08 |
| WO2008073184A3 (en) | 2008-11-27 |
| IL197917A0 (en) | 2011-08-01 |
| US20100021963A1 (en) | 2010-01-28 |
| AU2007333010A1 (en) | 2008-06-19 |
| EP2069481A2 (en) | 2009-06-17 |
| CN101535338A (zh) | 2009-09-16 |
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