RU2009112104A - Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих - Google Patents
Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих Download PDFInfo
- Publication number
- RU2009112104A RU2009112104A RU2009112104/10A RU2009112104A RU2009112104A RU 2009112104 A RU2009112104 A RU 2009112104A RU 2009112104/10 A RU2009112104/10 A RU 2009112104/10A RU 2009112104 A RU2009112104 A RU 2009112104A RU 2009112104 A RU2009112104 A RU 2009112104A
- Authority
- RU
- Russia
- Prior art keywords
- trna
- amino acid
- host cell
- translation system
- specified
- Prior art date
Links
- 150000001413 amino acids Chemical class 0.000 title claims abstract 35
- 102000004169 proteins and genes Human genes 0.000 title claims 9
- 108090000623 proteins and genes Proteins 0.000 title claims 9
- 241000124008 Mammalia Species 0.000 title 1
- 230000002068 genetic effect Effects 0.000 title 1
- 230000010354 integration Effects 0.000 title 1
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims abstract 13
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims abstract 13
- 108091033319 polynucleotide Proteins 0.000 claims abstract 10
- 102000040430 polynucleotide Human genes 0.000 claims abstract 10
- 239000002157 polynucleotide Substances 0.000 claims abstract 10
- DOCYTUNUHIGJTI-QMMMGPOBSA-N (2r)-2-[(2-nitrophenyl)methylamino]-3-sulfanylpropanoic acid Chemical compound OC(=O)[C@H](CS)NCC1=CC=CC=C1[N+]([O-])=O DOCYTUNUHIGJTI-QMMMGPOBSA-N 0.000 claims abstract 6
- ZXSBHXZKWRIEIA-JTQLQIEISA-N (2s)-3-(4-acetylphenyl)-2-azaniumylpropanoate Chemical compound CC(=O)C1=CC=C(C[C@H](N)C(O)=O)C=C1 ZXSBHXZKWRIEIA-JTQLQIEISA-N 0.000 claims abstract 6
- 241000288906 Primates Species 0.000 claims abstract 5
- 241000283984 Rodentia Species 0.000 claims abstract 4
- VKBLQCDGTHFOLS-NSHDSACASA-N (2s)-2-(4-benzoylanilino)propanoic acid Chemical compound C1=CC(N[C@@H](C)C(O)=O)=CC=C1C(=O)C1=CC=CC=C1 VKBLQCDGTHFOLS-NSHDSACASA-N 0.000 claims abstract 3
- NEMHIKRLROONTL-QMMMGPOBSA-N (2s)-2-azaniumyl-3-(4-azidophenyl)propanoate Chemical compound OC(=O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1 NEMHIKRLROONTL-QMMMGPOBSA-N 0.000 claims abstract 3
- AKVBCGQVQXPRLD-UHFFFAOYSA-N 2-aminooctanoic acid Chemical compound CCCCCCC(N)C(O)=O AKVBCGQVQXPRLD-UHFFFAOYSA-N 0.000 claims abstract 3
- 241000588724 Escherichia coli Species 0.000 claims abstract 3
- 241001425800 Pipa Species 0.000 claims abstract 3
- 101100191375 Xenopus laevis prkra-b gene Proteins 0.000 claims abstract 3
- 241000186394 Eubacterium Species 0.000 claims abstract 2
- GEYBMYRBIABFTA-VIFPVBQESA-N O-methyl-L-tyrosine Chemical compound COC1=CC=C(C[C@H](N)C(O)=O)C=C1 GEYBMYRBIABFTA-VIFPVBQESA-N 0.000 claims abstract 2
- 230000000689 aminoacylating effect Effects 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims 17
- 108020004705 Codon Proteins 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 238000002741 site-directed mutagenesis Methods 0.000 claims 2
- QHGDJQUCSGUYMF-QMMMGPOBSA-N (2s)-3-hydroxy-2-[(2-nitrophenyl)methylamino]propanoic acid Chemical compound OC[C@@H](C(O)=O)NCC1=CC=CC=C1[N+]([O-])=O QHGDJQUCSGUYMF-QMMMGPOBSA-N 0.000 claims 1
- PZNQZSRPDOEBMS-QMMMGPOBSA-N 4-iodo-L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(I)C=C1 PZNQZSRPDOEBMS-QMMMGPOBSA-N 0.000 claims 1
- 241000282693 Cercopithecidae Species 0.000 claims 1
- 241000282575 Gorilla Species 0.000 claims 1
- 241000282620 Hylobates sp. Species 0.000 claims 1
- 241000282553 Macaca Species 0.000 claims 1
- 241000282576 Pan paniscus Species 0.000 claims 1
- 241000282577 Pan troglodytes Species 0.000 claims 1
- 241000282405 Pongo abelii Species 0.000 claims 1
- 241000288961 Saguinus imperator Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- RCRODHONKLSMIF-UHFFFAOYSA-N isosuberenol Natural products O1C(=O)C=CC2=C1C=C(OC)C(CC(O)C(C)=C)=C2 RCRODHONKLSMIF-UHFFFAOYSA-N 0.000 claims 1
- 230000004952 protein activity Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85300806P | 2006-10-18 | 2006-10-18 | |
| US60/853,008 | 2006-10-18 | ||
| US92345807P | 2007-04-12 | 2007-04-12 | |
| US60/923,458 | 2007-04-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2009112104A true RU2009112104A (ru) | 2010-11-27 |
Family
ID=39512252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2009112104/10A RU2009112104A (ru) | 2006-10-18 | 2007-10-17 | Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20080166766A1 (enExample) |
| EP (1) | EP2069481A4 (enExample) |
| JP (1) | JP2010506591A (enExample) |
| KR (1) | KR20090094812A (enExample) |
| CN (1) | CN101535338A (enExample) |
| AU (1) | AU2007333010A1 (enExample) |
| BR (1) | BRPI0718389A2 (enExample) |
| CA (1) | CA2665678A1 (enExample) |
| IL (1) | IL197917A0 (enExample) |
| MX (1) | MX2009004043A (enExample) |
| RU (1) | RU2009112104A (enExample) |
| WO (1) | WO2008073184A2 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2464532T3 (es) * | 2001-04-19 | 2014-06-03 | The Scripps Research Institute | Métodos y composiciones para la producción de pares ortogonales de ARNt-aminoacil-ARNt sintetasa |
| EP1558747B1 (en) * | 2002-10-16 | 2009-10-14 | The Scripps Research Institute | Glycoprotein synthesis |
| JP5642916B2 (ja) | 2003-04-17 | 2014-12-17 | ザ スクリプス リサーチ インスティテュート | 真核遺伝コードの拡張 |
| WO2005019415A2 (en) | 2003-07-07 | 2005-03-03 | The Scripps Research Institute | Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof |
| WO2010114615A2 (en) * | 2009-04-03 | 2010-10-07 | The Scripps Research Institute | A facile system for encoding unnatural amino acids in mammalian cells |
| EP2647721B1 (en) * | 2010-12-03 | 2019-02-27 | The University of Tokyo | Peptide with safer secondary structure, peptide library, and production methods for same |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| US9670521B2 (en) * | 2012-09-24 | 2017-06-06 | Medimmune Limited | Amino acid derivatives |
| US10131920B2 (en) * | 2013-03-15 | 2018-11-20 | Medimmune Limited | Nucleic acid molecules |
| CN109295025B (zh) * | 2014-09-03 | 2021-04-02 | 中国科学院生物物理研究所 | 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族 |
| CN105400816A (zh) * | 2015-11-24 | 2016-03-16 | 深圳市第二人民医院 | 用于膀胱癌检测的逻辑与门基因线路的构建方法 |
| GB201600512D0 (en) | 2016-01-12 | 2016-02-24 | Univ York | Recombinant protein production |
| CN112725282A (zh) * | 2016-01-27 | 2021-04-30 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| CN110520522A (zh) | 2017-02-17 | 2019-11-29 | 鲁比厄斯治疗法股份有限公司 | 功能化红系细胞 |
| KR102705871B1 (ko) | 2017-06-02 | 2024-09-11 | 암브룩스, 인코포레이티드 | 비천연 아미노산 함유 단백질 생성을 촉진하는 방법 및 조성물 |
| AU2018281334A1 (en) | 2017-06-06 | 2019-12-19 | Kindred Biosciences, Inc. | Erythropoietin and analogs for veterinary use |
| ES3056533T3 (en) | 2018-04-19 | 2026-02-23 | Elanco Us Inc | Variant asparaginase polypeptides for medical use |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| US12565646B2 (en) | 2019-06-21 | 2026-03-03 | Trustees Of Boston College | Enhanced platforms for unnatural amino acid incorporation in mammalian cells |
| CA3150276A1 (en) * | 2019-08-08 | 2021-02-11 | Brickbio, Inc. | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
| EP4045639A1 (en) * | 2019-10-15 | 2022-08-24 | Trustees of Boston College | Chimeric thermostable aminoacyl-trna synthetase for enhanced unnatural amino acid incorporation |
| CN114787359A (zh) * | 2019-12-12 | 2022-07-22 | 中外制药株式会社 | 产生含有非天然氨基酸的肽的方法 |
| US20230313129A1 (en) * | 2020-08-07 | 2023-10-05 | Brickbio, Inc. | Stable cell lines for site-specific incorporation of unnatural amino acids |
| CN113388654A (zh) * | 2021-06-04 | 2021-09-14 | 清华大学 | 一种基于线形模板无细胞体系非天然氨基酸嵌入方法 |
| CN114134141B (zh) * | 2021-11-03 | 2024-01-30 | 杭州嵌化合生医药科技有限公司 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
| CN119662697B (zh) * | 2024-12-20 | 2026-03-27 | 天津大学 | 缺失基因的酿酒酵母及在利用非天然氨基酸修饰蛋白质中的应用 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2464532T3 (es) | 2001-04-19 | 2014-06-03 | The Scripps Research Institute | Métodos y composiciones para la producción de pares ortogonales de ARNt-aminoacil-ARNt sintetasa |
| EP1558747B1 (en) | 2002-10-16 | 2009-10-14 | The Scripps Research Institute | Glycoprotein synthesis |
| MXPA05003983A (es) | 2002-10-16 | 2005-06-22 | Scripps Research Inst | Incorporacion en sitio especifico de cetoaminoacidos en proteinas. |
| CA2503749A1 (en) * | 2002-10-31 | 2004-05-13 | Riken | Method of expressing protein having unnatural amino acid integrated thereinto |
| EP1583816A4 (en) | 2002-12-22 | 2007-06-13 | Scripps Research Inst | PROTEIN ARRAYS |
| PL378110A1 (pl) * | 2003-02-14 | 2006-03-06 | Biogen Idec Ma Inc. | Kaseta ekspresyjna i wektor do przejściowej albo stabilnej ekspresji egzogennych cząsteczek |
| JP5642916B2 (ja) | 2003-04-17 | 2014-12-17 | ザ スクリプス リサーチ インスティテュート | 真核遺伝コードの拡張 |
| JP5275566B2 (ja) * | 2003-06-18 | 2013-08-28 | ザ スクリプス リサーチ インスティチュート | 反応性非天然アミノ酸遺伝コード付加 |
| WO2005019415A2 (en) | 2003-07-07 | 2005-03-03 | The Scripps Research Institute | Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof |
| WO2005007624A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof |
| EP1704242A4 (en) | 2003-07-07 | 2008-06-18 | Scripps Research Inst | COMPOSITIONS WITH PAIRS OF ORTHOGONAL LEUCYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF |
| CN101532046A (zh) | 2003-10-14 | 2009-09-16 | 斯克利普斯研究院 | 将氧化还原活性氨基酸位点特异地掺入蛋白质 |
| CA2549830A1 (en) | 2003-12-18 | 2006-01-05 | The Scripps Research Institute | Selective incorporation of 5-hyroxytryptophan into proteins in mammalian cells |
| BRPI0507169A (pt) * | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos |
| CA2567624A1 (en) | 2004-05-25 | 2005-12-08 | The Scripps Research Institute | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
| MX2007003142A (es) | 2004-09-21 | 2007-07-04 | Scripps Research Inst | Incorporacion in vivo de alquinil aminoacidos a proteinas en eubacterias. |
| US20090181858A1 (en) * | 2004-09-21 | 2009-07-16 | The Scripps Research Institute | Adding photoregulated amino acids to the genetic code |
| EP1794312A4 (en) | 2004-09-22 | 2009-08-12 | Scripps Research Inst | LOCAL LABELING OF PROTEINS FOR NMR STUDIES |
| WO2006045116A2 (en) | 2004-10-20 | 2006-04-27 | The Scripps Research Institute | In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria |
| MX2007004892A (es) | 2004-10-27 | 2007-08-16 | Scripps Research Inst | Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales. |
| CA2624290A1 (en) | 2005-10-12 | 2007-04-26 | Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
| MX2008011470A (es) | 2006-03-09 | 2008-09-24 | Scripps Research Inst | Sistemas para la expresion de componentes de traduccion ortogonales en celulas huesped eubacterianas. |
| US7790847B2 (en) | 2006-03-16 | 2010-09-07 | The Scripps Research Institute | Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine |
-
2007
- 2007-10-17 CN CNA2007800390157A patent/CN101535338A/zh active Pending
- 2007-10-17 AU AU2007333010A patent/AU2007333010A1/en not_active Abandoned
- 2007-10-17 WO PCT/US2007/022232 patent/WO2008073184A2/en not_active Ceased
- 2007-10-17 BR BRPI0718389-5A patent/BRPI0718389A2/pt not_active IP Right Cessation
- 2007-10-17 US US11/975,351 patent/US20080166766A1/en not_active Abandoned
- 2007-10-17 RU RU2009112104/10A patent/RU2009112104A/ru not_active Application Discontinuation
- 2007-10-17 JP JP2009533372A patent/JP2010506591A/ja active Pending
- 2007-10-17 EP EP07870795A patent/EP2069481A4/en not_active Withdrawn
- 2007-10-17 US US12/311,545 patent/US9624485B2/en not_active Expired - Fee Related
- 2007-10-17 CA CA002665678A patent/CA2665678A1/en not_active Abandoned
- 2007-10-17 MX MX2009004043A patent/MX2009004043A/es not_active Application Discontinuation
- 2007-10-17 KR KR1020097009346A patent/KR20090094812A/ko not_active Ceased
-
2009
- 2009-04-05 IL IL197917A patent/IL197917A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2069481A2 (en) | 2009-06-17 |
| JP2010506591A (ja) | 2010-03-04 |
| EP2069481A4 (en) | 2010-01-06 |
| WO2008073184A3 (en) | 2008-11-27 |
| CN101535338A (zh) | 2009-09-16 |
| BRPI0718389A2 (pt) | 2012-04-10 |
| WO2008073184A2 (en) | 2008-06-19 |
| KR20090094812A (ko) | 2009-09-08 |
| US20080166766A1 (en) | 2008-07-10 |
| US9624485B2 (en) | 2017-04-18 |
| US20100021963A1 (en) | 2010-01-28 |
| CA2665678A1 (en) | 2008-06-19 |
| MX2009004043A (es) | 2009-04-27 |
| IL197917A0 (en) | 2011-08-01 |
| AU2007333010A1 (en) | 2008-06-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20120606 |