BRPI0718389A2 - incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos - Google Patents

incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos Download PDF

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Publication number
BRPI0718389A2
BRPI0718389A2 BRPI0718389-5A BRPI0718389A BRPI0718389A2 BR PI0718389 A2 BRPI0718389 A2 BR PI0718389A2 BR PI0718389 A BRPI0718389 A BR PI0718389A BR PI0718389 A2 BRPI0718389 A2 BR PI0718389A2
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BR
Brazil
Prior art keywords
trna
amino acid
unnatural amino
host cell
cell
Prior art date
Application number
BRPI0718389-5A
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English (en)
Portuguese (pt)
Inventor
Wenshe Liu
Peter G Schultz
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Scripps Research Inst
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Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of BRPI0718389A2 publication Critical patent/BRPI0718389A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BRPI0718389-5A 2006-10-18 2007-10-17 incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos BRPI0718389A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US85300806P 2006-10-18 2006-10-18
US60/853.008 2006-10-18
US92345807P 2007-04-12 2007-04-12
US60/923.458 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Publications (1)

Publication Number Publication Date
BRPI0718389A2 true BRPI0718389A2 (pt) 2012-04-10

Family

ID=39512252

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0718389-5A BRPI0718389A2 (pt) 2006-10-18 2007-10-17 incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos

Country Status (12)

Country Link
US (2) US20080166766A1 (enExample)
EP (1) EP2069481A4 (enExample)
JP (1) JP2010506591A (enExample)
KR (1) KR20090094812A (enExample)
CN (1) CN101535338A (enExample)
AU (1) AU2007333010A1 (enExample)
BR (1) BRPI0718389A2 (enExample)
CA (1) CA2665678A1 (enExample)
IL (1) IL197917A0 (enExample)
MX (1) MX2009004043A (enExample)
RU (1) RU2009112104A (enExample)
WO (1) WO2008073184A2 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) * 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
EP2647721B1 (en) 2010-12-03 2019-02-27 The University of Tokyo Peptide with safer secondary structure, peptide library, and production methods for same
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
BR112015006368B1 (pt) * 2012-09-24 2022-02-08 Medimmune Limited Processo para produção de linha celular eucariótica estável
BR112015022641B1 (pt) * 2013-03-15 2023-02-28 Medimmune Limited Construção de dna e processo de preparação de uma proteína-alvo
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN112725282A (zh) * 2016-01-27 2021-04-30 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
AU2018221227A1 (en) 2017-02-17 2019-08-15 Rubius Therapeutics, Inc. Functionalized erythroid cells
CN117286182A (zh) * 2017-06-02 2023-12-26 Ambrx公司 促进含非天然氨基酸的蛋白质产生的方法和组合物
EP3634988A4 (en) 2017-06-06 2021-02-24 Kindred Biosciences, Inc. ERYTHROPOIETIN AND SIMILAR COMPOUNDS FOR VETERINARY USE
US20210062176A1 (en) 2018-04-19 2021-03-04 Kindred Biosciences, Inc. Variant Asparaginase Polypeptides for Medical Use
US20210356377A1 (en) * 2018-09-07 2021-11-18 Medimmune, Llc Methods Of Cell Selection
US20220325269A1 (en) * 2019-08-08 2022-10-13 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
JP2022552137A (ja) * 2019-10-15 2022-12-15 トラスティーズ オブ ボストン カレッジ 非天然アミノ酸組み込み増強のためのキメラ耐熱性アミノアシルtRNAシンテターゼ
WO2021117848A1 (ja) * 2019-12-12 2021-06-17 中外製薬株式会社 非天然アミノ酸を含むペプチドの製造方法
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697A (zh) * 2024-12-20 2025-03-21 天津大学 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1490483B1 (en) 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
DK1578949T3 (da) 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
CA2503749A1 (en) * 2002-10-31 2004-05-13 Riken Method of expressing protein having unnatural amino acid integrated thereinto
EP1583816A4 (en) 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
EA200700469A1 (ru) * 2003-02-14 2008-10-30 Байоджен Айдек Ма Инк. Экспрессионная кассета и вектор для временной или постоянной экспрессии экзогенных молекул
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
WO2005003294A2 (en) * 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005038002A2 (en) 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
BRPI0417801A (pt) 2003-12-18 2007-04-10 Scripps Research Inst incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas
BRPI0507159A (pt) 2004-02-02 2007-06-26 Ambrx Inc polipeptìdeos em feixe de quatro hélices humanos modificados e seus usos
AU2005248392A1 (en) 2004-05-25 2005-12-08 Irm, Llc Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination
EP1797177A4 (en) * 2004-09-21 2008-07-09 Scripps Research Inst ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE
EP1794323B1 (en) 2004-09-21 2014-08-06 The Scripps Research Institute In vivo incorporation of alkynyl amino acids into proteins in eubacteria
JP2008513806A (ja) 2004-09-22 2008-05-01 ザ スクリップス リサーチ インスティテュート Nmr試験のための蛋白質の部位特異的標識
EP1809758A4 (en) 2004-10-20 2009-04-01 Scripps Research Inst LOCAL IN VIVO INSTALLATION OF N-ACETYLGALACTOSAMINE AMINO ACIDS IN EU BACTERIA
AU2005330514B2 (en) 2004-10-27 2011-05-12 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CA2624290A1 (en) 2005-10-12 2007-04-26 Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CA2638763A1 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現

Also Published As

Publication number Publication date
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
RU2009112104A (ru) 2010-11-27
MX2009004043A (es) 2009-04-27
JP2010506591A (ja) 2010-03-04
WO2008073184A2 (en) 2008-06-19
EP2069481A4 (en) 2010-01-06
KR20090094812A (ko) 2009-09-08
WO2008073184A3 (en) 2008-11-27
IL197917A0 (en) 2011-08-01
US20100021963A1 (en) 2010-01-28
AU2007333010A1 (en) 2008-06-19
CA2665678A1 (en) 2008-06-19
EP2069481A2 (en) 2009-06-17
CN101535338A (zh) 2009-09-16

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2261 DE 06/05/2014.