CN101535338A - 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质 - Google Patents

在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质 Download PDF

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CN101535338A
CN101535338A CNA2007800390157A CN200780039015A CN101535338A CN 101535338 A CN101535338 A CN 101535338A CN A2007800390157 A CNA2007800390157 A CN A2007800390157A CN 200780039015 A CN200780039015 A CN 200780039015A CN 101535338 A CN101535338 A CN 101535338A
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trna
amino acid
amino acids
unnatural amino
host cell
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刘文设
P·G·舒尔茨
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Scripps Research Institute
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Gastroenterology & Hepatology (AREA)
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CNA2007800390157A 2006-10-18 2007-10-17 在哺乳动物细胞中将非天然氨基酸遗传掺入蛋白质 Pending CN101535338A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US85300806P 2006-10-18 2006-10-18
US60/853,008 2006-10-18
US92345807P 2007-04-12 2007-04-12
US60/923,458 2007-04-12

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CN101535338A true CN101535338A (zh) 2009-09-16

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US (2) US20080166766A1 (enExample)
EP (1) EP2069481A4 (enExample)
JP (1) JP2010506591A (enExample)
KR (1) KR20090094812A (enExample)
CN (1) CN101535338A (enExample)
AU (1) AU2007333010A1 (enExample)
BR (1) BRPI0718389A2 (enExample)
CA (1) CA2665678A1 (enExample)
IL (1) IL197917A0 (enExample)
MX (1) MX2009004043A (enExample)
RU (1) RU2009112104A (enExample)
WO (1) WO2008073184A2 (enExample)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103328648A (zh) * 2010-12-03 2013-09-25 国立大学法人东京大学 具有稳定的二级结构的肽和肽库以及它们的制造方法
CN110724707A (zh) * 2013-03-15 2020-01-24 米迪缪尼有限公司 新型核酸分子
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
CN112725282A (zh) * 2016-01-27 2021-04-30 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141A (zh) * 2021-11-03 2022-03-04 浙江大学 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN114555645A (zh) * 2019-10-15 2022-05-27 波士顿学院董事会 用于增强非天然氨基酸掺入的嵌合热稳定氨酰tRNA合成酶
CN114729334A (zh) * 2019-08-08 2022-07-08 布里克生物公司 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系
CN114787359A (zh) * 2019-12-12 2022-07-22 中外制药株式会社 产生含有非天然氨基酸的肽的方法
CN110959041B (zh) * 2017-06-02 2023-09-29 Ambrx公司 促进含非天然氨基酸的蛋白质产生的方法和组合物
CN119662697A (zh) * 2024-12-20 2025-03-21 天津大学 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用

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EP1490483B1 (en) * 2001-04-19 2015-06-03 The Scripps Research Institute In vivo incorporation of unnatural amino acids
KR20050073559A (ko) * 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
CN103289960B (zh) 2003-04-17 2017-04-26 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) * 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
BR112015006368B1 (pt) * 2012-09-24 2022-02-08 Medimmune Limited Processo para produção de linha celular eucariótica estável
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
AU2018221227A1 (en) 2017-02-17 2019-08-15 Rubius Therapeutics, Inc. Functionalized erythroid cells
EP3634988A4 (en) 2017-06-06 2021-02-24 Kindred Biosciences, Inc. ERYTHROPOIETIN AND SIMILAR COMPOUNDS FOR VETERINARY USE
US20210062176A1 (en) 2018-04-19 2021-03-04 Kindred Biosciences, Inc. Variant Asparaginase Polypeptides for Medical Use

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KR20050073559A (ko) 2002-10-16 2005-07-14 더 스크립스 리서치 인스티튜트 당단백질 합성
DK1578949T3 (da) 2002-10-16 2009-07-27 Scripps Research Inst Stedspecifik optagelse af keto-aminnosyrer hos proteiner
CA2503749A1 (en) * 2002-10-31 2004-05-13 Riken Method of expressing protein having unnatural amino acid integrated thereinto
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EA200700469A1 (ru) * 2003-02-14 2008-10-30 Байоджен Айдек Ма Инк. Экспрессионная кассета и вектор для временной или постоянной экспрессии экзогенных молекул
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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103328648B (zh) * 2010-12-03 2015-11-25 国立大学法人东京大学 具有稳定的二级结构的肽和肽库以及它们的制造方法
CN103328648A (zh) * 2010-12-03 2013-09-25 国立大学法人东京大学 具有稳定的二级结构的肽和肽库以及它们的制造方法
CN110724707A (zh) * 2013-03-15 2020-01-24 米迪缪尼有限公司 新型核酸分子
CN112725282A (zh) * 2016-01-27 2021-04-30 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN110959041B (zh) * 2017-06-02 2023-09-29 Ambrx公司 促进含非天然氨基酸的蛋白质产生的方法和组合物
CN112673109A (zh) * 2018-09-07 2021-04-16 免疫医疗有限责任公司 细胞选择方法
CN114729334A (zh) * 2019-08-08 2022-07-08 布里克生物公司 用于非天然氨基酸的位点特异性掺入的氨酰tRNA合成酶和细胞系
CN114555645A (zh) * 2019-10-15 2022-05-27 波士顿学院董事会 用于增强非天然氨基酸掺入的嵌合热稳定氨酰tRNA合成酶
CN114787359A (zh) * 2019-12-12 2022-07-22 中外制药株式会社 产生含有非天然氨基酸的肽的方法
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141A (zh) * 2021-11-03 2022-03-04 浙江大学 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN119662697A (zh) * 2024-12-20 2025-03-21 天津大学 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用

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US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
RU2009112104A (ru) 2010-11-27
MX2009004043A (es) 2009-04-27
JP2010506591A (ja) 2010-03-04
WO2008073184A2 (en) 2008-06-19
EP2069481A4 (en) 2010-01-06
BRPI0718389A2 (pt) 2012-04-10
KR20090094812A (ko) 2009-09-08
WO2008073184A3 (en) 2008-11-27
IL197917A0 (en) 2011-08-01
US20100021963A1 (en) 2010-01-28
AU2007333010A1 (en) 2008-06-19
CA2665678A1 (en) 2008-06-19
EP2069481A2 (en) 2009-06-17

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Application publication date: 20090916