JP2004514431A5 - - Google Patents

Info

Publication number

JP2004514431A5

JP2004514431A5 JP2002545124A JP2002545124A JP2004514431A5 JP 2004514431 A5 JP2004514431 A5 JP 2004514431A5 JP 2002545124 A JP2002545124 A JP 2002545124A JP 2002545124 A JP2002545124 A JP 2002545124A JP 2004514431 A5 JP2004514431 A5 JP 2004514431A5

Authority

JP

Japan

Prior art keywords

coa

activity

polypeptide

cell

contacting

Prior art date

2001-11-20

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 230000000694 effects Effects 0.000 claims description 154
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 100
• 229920001184 polypeptide Polymers 0.000 claims description 97
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 97
• 210000004027 cell Anatomy 0.000 claims description 96
• VIWKEBOLLIEAIL-FBMOWMAESA-N lactoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C(O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VIWKEBOLLIEAIL-FBMOWMAESA-N 0.000 claims description 36
• 150000007523 nucleic acids Chemical group 0.000 claims description 34
• 101710088194 Dehydrogenase Proteins 0.000 claims description 33
• 238000000034 method Methods 0.000 claims description 29
• 108020004707 nucleic acids Proteins 0.000 claims description 26
• 102000039446 nucleic acids Human genes 0.000 claims description 26
• 108090000364 Ligases Proteins 0.000 claims description 24
• 102000003960 Ligases Human genes 0.000 claims description 24
• POODSGUMUCVRTR-IEXPHMLFSA-N acryloyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C=C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 POODSGUMUCVRTR-IEXPHMLFSA-N 0.000 claims description 24
• 238000004519 manufacturing process Methods 0.000 claims description 22
• 238000012258 culturing Methods 0.000 claims description 18
• 150000002148 esters Chemical class 0.000 claims description 18
• ALRHLSYJTWAHJZ-UHFFFAOYSA-N 3-hydroxypropionic acid Chemical compound OCCC(O)=O ALRHLSYJTWAHJZ-UHFFFAOYSA-N 0.000 claims description 16
• 102000004190 Enzymes Human genes 0.000 claims description 16
• 108090000790 Enzymes Proteins 0.000 claims description 16
• 102000004157 Hydrolases Human genes 0.000 claims description 16
• 108090000604 Hydrolases Proteins 0.000 claims description 16
• JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 14
• 102000004357 Transferases Human genes 0.000 claims description 14
• 108090000992 Transferases Proteins 0.000 claims description 14
• DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 claims description 12
• YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims description 12
• NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims description 12
• 108010067653 lactate dehydratase Proteins 0.000 claims description 12
• 229920000166 polytrimethylene carbonate Polymers 0.000 claims description 12
• 229920001273 Polyhydroxy acid Polymers 0.000 claims description 10
• -1 ethyl 3-hydroxy propionate Chemical compound 0.000 claims description 10
• 230000002366 lipolytic effect Effects 0.000 claims description 10
• BERBFZCUSMQABM-IEXPHMLFSA-N 3-hydroxypropanoyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCO)O[C@H]1N1C2=NC=NC(N)=C2N=C1 BERBFZCUSMQABM-IEXPHMLFSA-N 0.000 claims description 9
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 8
• 241000588724 Escherichia coli Species 0.000 claims description 8
• LTYOQGRJFJAKNA-KKIMTKSISA-N Malonyl CoA Natural products S(C(=O)CC(=O)O)CCNC(=O)CCNC(=O)[C@@H](O)C(CO[P@](=O)(O[P@](=O)(OC[C@H]1[C@@H](OP(=O)(O)O)[C@@H](O)[C@@H](n2c3ncnc(N)c3nc2)O1)O)O)(C)C LTYOQGRJFJAKNA-KKIMTKSISA-N 0.000 claims description 8
• 102000004316 Oxidoreductases Human genes 0.000 claims description 8
• 108090000854 Oxidoreductases Proteins 0.000 claims description 8
• ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 claims description 8
• UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 claims description 8
• LTYOQGRJFJAKNA-DVVLENMVSA-N malonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 LTYOQGRJFJAKNA-DVVLENMVSA-N 0.000 claims description 8
• 108030005924 3-hydroxypropionyl-CoA dehydratases Proteins 0.000 claims description 7
• 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
• 108010008386 malonyl-Coa reductase Proteins 0.000 claims description 6
• SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 4
• NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 4
• 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 4
• 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 4
• 102000005369 Aldehyde Dehydrogenase Human genes 0.000 claims description 4
• 108020002663 Aldehyde Dehydrogenase Proteins 0.000 claims description 4
• 241000186660 Lactobacillus Species 0.000 claims description 4
• 241000194036 Lactococcus Species 0.000 claims description 4
• 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
• 125000000539 amino acid group Chemical group 0.000 claims description 4
• 229940000635 beta-alanine Drugs 0.000 claims description 4
• 239000002773 nucleotide Substances 0.000 claims description 4
• 125000003729 nucleotide group Chemical group 0.000 claims description 4
• 230000002255 enzymatic effect Effects 0.000 claims description 3
• RVGLEPQPVDUSOJ-UHFFFAOYSA-N 2-Methyl-3-hydroxypropanoate Chemical group COC(=O)CCO RVGLEPQPVDUSOJ-UHFFFAOYSA-N 0.000 claims description 2
• 102000000452 Acetyl-CoA carboxylase Human genes 0.000 claims description 2
• 108010016219 Acetyl-CoA carboxylase Proteins 0.000 claims description 2
• 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 claims description 2
• 108030006993 Beta-alanyl-CoA ammonia-lyases Proteins 0.000 claims description 2
• 108010018763 Biotin carboxylase Proteins 0.000 claims description 2
• 102000016901 Glutamate dehydrogenase Human genes 0.000 claims description 2
• 150000001252 acrylic acid derivatives Chemical class 0.000 claims description 2
• 125000005396 acrylic acid ester group Chemical group 0.000 claims description 2
• 239000012190 activator Substances 0.000 claims description 2
• 150000001413 amino acids Chemical group 0.000 claims description 2
• 239000011230 binding agent Substances 0.000 claims description 2
• 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 2
• 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
• 230000009870 specific binding Effects 0.000 claims description 2
• 238000006467 substitution reaction Methods 0.000 claims description 2
• 230000018044 dehydration Effects 0.000 description 1
• 238000006297 dehydration reaction Methods 0.000 description 1