JP2008516612A5 - - Google Patents

Download PDF

Info

Publication number
JP2008516612A5
JP2008516612A5 JP2007537034A JP2007537034A JP2008516612A5 JP 2008516612 A5 JP2008516612 A5 JP 2008516612A5 JP 2007537034 A JP2007537034 A JP 2007537034A JP 2007537034 A JP2007537034 A JP 2007537034A JP 2008516612 A5 JP2008516612 A5 JP 2008516612A5
Authority
JP
Japan
Prior art keywords
probe
reaction mixture
low temperature
detecting
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2007537034A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008516612A (ja
JP5543065B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2005/037667 external-priority patent/WO2006044994A2/en
Publication of JP2008516612A publication Critical patent/JP2008516612A/ja
Publication of JP2008516612A5 publication Critical patent/JP2008516612A5/ja
Application granted granted Critical
Publication of JP5543065B2 publication Critical patent/JP5543065B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

JP2007537034A 2004-10-18 2005-10-17 核酸増幅のためのプライマー、プローブおよび方法 Expired - Lifetime JP5543065B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
US60/619,654 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2014000518A Division JP5860484B2 (ja) 2004-10-18 2014-01-06 核酸増幅のためのプライマー、プローブおよび方法

Publications (3)

Publication Number Publication Date
JP2008516612A JP2008516612A (ja) 2008-05-22
JP2008516612A5 true JP2008516612A5 (enExample) 2012-01-19
JP5543065B2 JP5543065B2 (ja) 2014-07-09

Family

ID=36203686

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2007537034A Expired - Lifetime JP5543065B2 (ja) 2004-10-18 2005-10-17 核酸増幅のためのプライマー、プローブおよび方法
JP2014000518A Expired - Lifetime JP5860484B2 (ja) 2004-10-18 2014-01-06 核酸増幅のためのプライマー、プローブおよび方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2014000518A Expired - Lifetime JP5860484B2 (ja) 2004-10-18 2014-01-06 核酸増幅のためのプライマー、プローブおよび方法

Country Status (17)

Country Link
US (3) US7632642B2 (enExample)
EP (3) EP1805199B1 (enExample)
JP (2) JP5543065B2 (enExample)
KR (3) KR101424812B1 (enExample)
CN (1) CN101076607B (enExample)
AT (1) ATE494295T1 (enExample)
AU (1) AU2005295298B2 (enExample)
BR (1) BRPI0517105B1 (enExample)
CA (3) CA2996947C (enExample)
DE (1) DE602005025791D1 (enExample)
DK (1) DK1805199T3 (enExample)
ES (3) ES2668467T3 (enExample)
NO (1) NO20072506L (enExample)
PL (1) PL1805199T3 (enExample)
PT (1) PT1805199E (enExample)
RU (1) RU2460804C2 (enExample)
WO (1) WO2006044994A2 (enExample)

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2668467T3 (es) * 2004-10-18 2018-05-18 Brandeis University Métodos de amplificación LATE y secuenciación de un ácido nucleico
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
AU2008275597A1 (en) 2007-07-12 2009-01-15 Smiths Detection Inc. Fiber optic detection system
EP2215259A1 (en) 2007-10-23 2010-08-11 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
US9856522B2 (en) * 2008-12-30 2018-01-02 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムｐｃｒ装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
EP2406394B1 (en) * 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr
US8674080B2 (en) * 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
AR077840A1 (es) 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
JP5499037B2 (ja) * 2009-08-14 2014-05-21 株式会社日立製作所 遺伝子配列解析法及び試薬
US9353407B2 (en) 2009-10-21 2016-05-31 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
JP2013509863A (ja) * 2009-11-03 2013-03-21 エイチティージー モレキュラー ダイアグノスティクス， インコーポレイテッド 定量的ヌクレアーゼ保護シークエンシング（ｑＮＰＳ）
US20140186827A1 (en) * 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
US20120058481A1 (en) * 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
EP2614157A4 (en) * 2010-09-08 2014-03-26 Univ Brandeis Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
RU2565550C2 (ru) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Прямой захват, амплификация и секвенирование днк-мишени с использованием иммобилизированных праймеров
US20130295570A1 (en) * 2010-10-07 2013-11-07 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP2638178B1 (en) 2010-11-10 2016-10-05 Brandeis University Methods, and kits for detecting and identifying mycobacteria
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
EP2649201A4 (en) * 2010-12-10 2014-10-01 Univ Brandeis COMPOSITIONS AND METHODS FOR DETECTING AND ANALYZING AFRICAN SWINE FEVER
EP2659002B1 (en) 2010-12-27 2017-02-01 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
AU2012251027B2 (en) 2011-05-04 2015-03-26 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection Assay (qNPA) and sequencing (qNPS) improvements
WO2013019759A1 (en) * 2011-07-30 2013-02-07 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
WO2013040491A2 (en) 2011-09-15 2013-03-21 Shafer David A Probe: antiprobe compositions for high specificity dna or rna detection
EP2774995A4 (en) * 2011-10-31 2015-04-29 Arkray Inc METHOD OF MEASURING THE ABUNDANCE OF GENES
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
WO2013131083A1 (en) 2012-03-02 2013-09-06 Winthrop-University Hospital METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED β CELL DERIVED DNA AS A MEASURE OF β CELL LOSS IN DIABETES
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
IN2015DN01609A (enExample) * 2012-08-03 2015-07-03 California Inst Of Techn
WO2014043660A2 (en) * 2012-09-17 2014-03-20 Brandeis University Combination of dsdna binding dye and probes for characterization of ssdna sequences
EP2959016A4 (en) * 2013-02-21 2016-10-12 Toma Biosciences Inc METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
EP3666903B1 (en) 2013-08-12 2025-12-31 Bio-Rad Laboratories, Inc. AMPLIFICATION REPORTER GENE CONTAINING OLIGOMERS WITH BASIC PAIRINGS
ES2875998T3 (es) * 2013-09-30 2021-11-11 Vesicode Ab Procedimientos de perfilado de complejos moleculares mediante el uso de códigos de barras dependientes de la proximidad
WO2015086604A1 (en) * 2013-12-11 2015-06-18 Qiagen Gmbh Nucleic acid detection and quantification
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
WO2016040523A1 (en) * 2014-09-12 2016-03-17 Click Diagnostics, Inc. Multiplex optical detection
EP3204517B1 (en) 2014-10-10 2021-08-11 Rutgers, the State University of New Jersey Polymerase chain reaction primers and probes for mycobacterium tuberculosis
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
EP3234186A1 (en) * 2014-12-19 2017-10-25 Brandeis University Mispriming prevention reagents
ES2898103T3 (es) 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
EP3247797B1 (en) * 2015-01-12 2020-04-29 Life Technologies Corporation Methods, systems and compositions thereof for nucleic acid library quality control and quantification
KR102165931B1 (ko) * 2015-09-24 2020-10-14 주식회사 씨젠 타겟 분석물질의 존재 또는 부존재를 결정하기 위한 다중 데이터 세트 분석법
WO2017087942A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting rna and dna sequences comprising high levels of intramolecular hybridization
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN109923613B (zh) * 2016-06-02 2023-07-04 Seegene株式会社 利用信号变化量数据集的样品内的目标分析物质检测方法
EP3478857A1 (en) 2016-06-29 2019-05-08 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
CN110582577B (zh) * 2017-04-11 2025-03-21 帝肯基因组学公司 文库定量和鉴定
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
KR102380264B1 (ko) * 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
CN111655866A (zh) 2017-11-09 2020-09-11 维斯比医学公司 便携式分子诊断装置和检测靶病毒的方法
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅（武汉）生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
EP3743211A4 (en) * 2018-01-22 2021-11-24 Luminex Corporation METHODS AND COMPOSITIONS FOR THE ANALYSIS OF DISCRETE MELT
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
EP3781708B1 (en) 2018-04-20 2025-01-15 Seegene, Inc. Method for detecting a plurality of target nucleic acid sequences in sample
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP2021534804A (ja) 2018-09-03 2021-12-16 ビスビュー メディカル，インコーポレイテッド 抗生物質感受性検査のための装置および方法
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
SG11202110286PA (en) 2019-04-28 2021-10-28 Visby Medical Inc Molecular diagnostic devices with digital detection capability and wireless connectivity
US20220259641A1 (en) * 2019-07-17 2022-08-18 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
AU2020354419B2 (en) 2019-09-23 2025-12-04 Universiteit Gent Probe and method for STR-genotyping
WO2021138544A1 (en) 2020-01-03 2021-07-08 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术（深圳）有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
WO2023023533A1 (en) * 2021-08-19 2023-02-23 Luminex Corporation Digital amplification assay analysis method
US12516389B2 (en) 2021-11-19 2026-01-06 Stmicroelectronics S.R.L. Microdot array having PCR-primers fixed in each microdot and method of forming the same on a substrate for gene based pathogen detection
JP7852045B2 (ja) * 2022-06-20 2026-04-27 株式会社日立ハイテク プライマー、ｄｎａ検出方法およびｄｎａ検出キット
WO2026024668A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Method of determining a volume of a liquid in a sample
WO2026024677A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Method for determining the plasma volume of a blood sample and method for determining the dilution of plasma
WO2026024666A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Systems and methods for digital detection assays

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
ATE428801T1 (de) * 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
EP1210358A4 (en) 1999-08-13 2005-01-05 Univ Brandeis PROOF OF NUCLEIC ACIDS
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
DE60030145T2 (de) 1999-10-22 2008-01-10 PHRI Properties, Inc., Newark Nachweisverfahren für kurze sequenzvarianten
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
WO2002101004A2 (en) * 2001-06-08 2002-12-19 Shanghai Mendel Dna Center Co., Ltd Low-temperature cycle extension of dna with high priming specificity
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
WO2003040397A2 (en) * 2001-10-25 2003-05-15 Gorilla Genomics, Inc. Asymmetric pcr with nuclease-free polymerase or nuclease-resistant molecular beacons
AU2002346484A1 (en) 2001-11-21 2003-06-10 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
EP1394271B1 (en) * 2002-09-02 2007-11-14 Toyo Boseki Kabushiki Kaisha Method for identifying nucleotide polymorphisms using resonance energy transfer
US7718783B2 (en) * 2002-11-22 2010-05-18 Kaplan Mark H ELF3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
EP1709200B1 (en) * 2003-12-03 2012-05-09 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
EP3611273A1 (en) * 2004-03-31 2020-02-19 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
ES2668467T3 (es) * 2004-10-18 2018-05-18 Brandeis University Métodos de amplificación LATE y secuenciación de un ácido nucleico

Similar Documents

Publication Publication Date Title
Jiang et al. G-quadruplex fluorescent probe-mediated real-time rolling circle amplification strategy for highly sensitive microRNA detection
JP6144623B2 (ja) 核酸測定用の核酸プローブ
WO2006128010A3 (en) Quantification of nucleic acids and proteins using oligonucleotide mass tags
JP2011530296A5 (enExample)
JP2021511056A5 (enExample)
WO2008046645A3 (en) A new method for qualitative and quantitative detection of short nucleic acid sequences of about 8-50 nucleotides in length
WO2004059009A1 (en) A method of real-time detection of nucleic acid sequences
CA2790153A1 (en) Thd primer target detection
WO2007087336A3 (en) Methods for detecting nucleic acids using multiple signals
RU2007132732A (ru) СПОСОБ КОЛИЧЕСТВЕННОГО АНАЛИЗА МИКРООРГАНИЗМА С ПРИМЕНЕНИЕМ pPHK В КАЧЕСТВЕ МИШЕНИ
US20060035225A1 (en) Method for detecting target nucleic acid
EP1375674A1 (en) Method of detecting nucleic acids using probes with fluorescent labels by analysis of the fluorescence melting curves
JP2018503390A (ja) 基質分子
JP5593582B2 (ja) 核酸の迅速な検出方法
JP2010017127A5 (enExample)
JP2008535478A (ja) シグナル増幅による核酸の検出および分析のための方法および組成物
JP5096007B2 (ja) 核酸プローブセットを用いるリアルタイムｐｃｒ方法
KR101438747B1 (ko) 신규한 형광성 핵산 염색용 조성물 및 이를 이용한 핵산 증폭의 측정방법
JP6720161B2 (ja) 複数の標的核酸の検出キット及びそれを用いる検出方法
KR101899371B1 (ko) 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
JP2009077736A (ja) 核酸の新規測定方法
JP6435568B2 (ja) Ａｃｉｎｅｔｏｂａｃｔｅｒ ｂａｕｍａｎｎｉｉの検出
US6872532B2 (en) Method and kit for detecting white spot syndrome virus
JP5670396B2 (ja) 核酸プローブセットおよびその使用方法
WO2008021010A2 (en) Methods for real-time quantitative pcr