JP2008516612A5 - - Google Patents

Download PDF

Info

Publication number
JP2008516612A5
JP2008516612A5 JP2007537034A JP2007537034A JP2008516612A5 JP 2008516612 A5 JP2008516612 A5 JP 2008516612A5 JP 2007537034 A JP2007537034 A JP 2007537034A JP 2007537034 A JP2007537034 A JP 2007537034A JP 2008516612 A5 JP2008516612 A5 JP 2008516612A5
Authority
JP
Japan
Prior art keywords
probe
reaction mixture
low temperature
detecting
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2007537034A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008516612A (ja
JP5543065B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2005/037667 external-priority patent/WO2006044994A2/en
Publication of JP2008516612A publication Critical patent/JP2008516612A/ja
Publication of JP2008516612A5 publication Critical patent/JP2008516612A5/ja
Application granted granted Critical
Publication of JP5543065B2 publication Critical patent/JP5543065B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

JP2007537034A 2004-10-18 2005-10-17 核酸増幅のためのプライマー、プローブおよび方法 Expired - Lifetime JP5543065B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
US60/619,654 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2014000518A Division JP5860484B2 (ja) 2004-10-18 2014-01-06 核酸増幅のためのプライマー、プローブおよび方法

Publications (3)

Publication Number Publication Date
JP2008516612A JP2008516612A (ja) 2008-05-22
JP2008516612A5 true JP2008516612A5 (enExample) 2012-01-19
JP5543065B2 JP5543065B2 (ja) 2014-07-09

Family

ID=36203686

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2007537034A Expired - Lifetime JP5543065B2 (ja) 2004-10-18 2005-10-17 核酸増幅のためのプライマー、プローブおよび方法
JP2014000518A Expired - Lifetime JP5860484B2 (ja) 2004-10-18 2014-01-06 核酸増幅のためのプライマー、プローブおよび方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2014000518A Expired - Lifetime JP5860484B2 (ja) 2004-10-18 2014-01-06 核酸増幅のためのプライマー、プローブおよび方法

Country Status (17)

Country Link
US (3) US7632642B2 (enExample)
EP (3) EP1805199B1 (enExample)
JP (2) JP5543065B2 (enExample)
KR (3) KR101424812B1 (enExample)
CN (1) CN101076607B (enExample)
AT (1) ATE494295T1 (enExample)
AU (1) AU2005295298B2 (enExample)
BR (1) BRPI0517105B1 (enExample)
CA (3) CA2855748C (enExample)
DE (1) DE602005025791D1 (enExample)
DK (1) DK1805199T3 (enExample)
ES (3) ES2668467T3 (enExample)
NO (1) NO20072506L (enExample)
PL (1) PL1805199T3 (enExample)
PT (1) PT1805199E (enExample)
RU (1) RU2460804C2 (enExample)
WO (1) WO2006044994A2 (enExample)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101424812B1 (ko) 2004-10-18 2014-08-07 브랜데이스 유니버시티 핵산 증폭을 위한 프라이머, 프로브 및 방법
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
US7933011B2 (en) 2007-07-12 2011-04-26 Smiths Detection Inc. Fiber optic detection system
CN101910410A (zh) * 2007-10-23 2010-12-08 斯特拉托斯基因公司 通过间隔进行高通量核酸测序
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
US9856522B2 (en) 2008-12-30 2018-01-02 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムpcr装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
US8674080B2 (en) 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077840A1 (es) 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
CN102471364B (zh) * 2009-08-14 2015-01-14 株式会社日立制作所 基因序列分析的方法和试剂
EP2980224B1 (en) 2009-10-21 2019-07-03 Brandeis University Kits for analyzing single-stranded nucleic acid sequences
EP2496715B1 (en) * 2009-11-03 2016-01-27 HTG Molecular Diagnostics, Inc. Quantitative nuclease protection sequencing (qnps)
EP2569447A4 (en) * 2010-05-14 2013-11-27 Fluidigm Corp ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
EP2606150B1 (en) * 2010-08-20 2016-03-16 Life Technologies Corporation Quantitative real-time pcr assay using fret dual-labeled primers
US9850529B2 (en) 2010-09-08 2017-12-26 Brandeis University Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
US9309556B2 (en) 2010-09-24 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US20130295570A1 (en) * 2010-10-07 2013-11-07 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
US10273525B2 (en) 2010-11-10 2019-04-30 Brandeis University Compositions, methods, and kits for detecting and identifying mycobacteria
WO2012075230A1 (en) 2010-12-03 2012-06-07 Brandeis University Detecting mutations in dna
EP2649201A4 (en) * 2010-12-10 2014-10-01 Univ Brandeis COMPOSITIONS AND METHODS FOR DETECTING AND ANALYZING AFRICAN SWINE FEVER
EP3181704B1 (en) 2010-12-27 2021-01-27 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
CN103649335B (zh) 2011-05-04 2015-11-25 Htg分子诊断有限公司 定量核酸酶保护测定(qnpa)和测序(qnps)的改进
US20150031024A1 (en) * 2011-07-30 2015-01-29 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
CA2849023C (en) 2011-09-15 2022-07-19 David A. Shafer Probe:antiprobe compositions for high specificity dna or rna detection
CN104024433B (zh) * 2011-10-31 2016-11-16 爱科来株式会社 基因丰度的测定方法
CN104271767B (zh) 2012-02-03 2017-08-25 加州理工学院 多路生化测定中信号的编码和解码
WO2013131083A1 (en) 2012-03-02 2013-09-06 Winthrop-University Hospital METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED β CELL DERIVED DNA AS A MEASURE OF β CELL LOSS IN DIABETES
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
IN2015DN01609A (enExample) 2012-08-03 2015-07-03 California Inst Of Techn
WO2014043660A2 (en) * 2012-09-17 2014-03-20 Brandeis University Combination of dsdna binding dye and probes for characterization of ssdna sequences
CA2902207A1 (en) * 2013-02-21 2014-08-28 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
EP2971095B1 (en) 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
CN105378160B (zh) 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
DK3052658T3 (da) * 2013-09-30 2021-06-07 Vesicode Ab Fremgangsmåder til profilering af molekylkomplekser ved anvendelse af proksimitetsafhængig stregkodning
US10501783B2 (en) 2013-12-11 2019-12-10 Qiagen Gmbh Nucleic acid detection and quantification
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170247745A1 (en) * 2014-09-12 2017-08-31 Click Diagnostics, Inc. Multiplex optical detection
CN116042880A (zh) 2014-10-10 2023-05-02 新泽西鲁特格斯州立大学 结核分枝杆菌的聚合酶链反应引物和探针
US10876155B2 (en) 2014-12-09 2020-12-29 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
EP3234186A1 (en) 2014-12-19 2017-10-25 Brandeis University Mispriming prevention reagents
EP3240906B1 (en) 2014-12-31 2021-08-25 Visby Medical, Inc. Devices for molecular diagnostic testing
WO2016115001A2 (en) * 2015-01-12 2016-07-21 Life Technologies Corporation Methods, systems and compositions thereof for nucleic acid library quality control and quantification
EP3353697B1 (en) * 2015-09-24 2025-11-05 Seegene, Inc. Multiple dataset analysis for determining the presence or absence of target analyte
WO2017087943A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
KR102326604B1 (ko) * 2016-06-02 2021-11-15 주식회사 씨젠 신호 변화량 데이터 세트를 이용한 샘플 내 타겟 분석물질 검출 방법
WO2018005710A1 (en) 2016-06-29 2018-01-04 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
JP6983906B2 (ja) * 2017-04-11 2021-12-17 ニューゲン テクノロジーズ, インコーポレイテッド ライブラリーの定量および定性
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
EP3676400A4 (en) * 2017-08-31 2021-06-02 Seegene, Inc. EVALUATING THE PERFORMANCE OF COMPONENTS USING A PAIR OF DIMERING PRIMERS
WO2019094784A1 (en) 2017-11-09 2019-05-16 Click Diagnostics, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅(武汉)生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
JP2021511056A (ja) 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物
WO2019204357A1 (en) 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
BR112020019755A2 (pt) * 2018-04-20 2021-01-26 Seegene, Inc. método e aparelho para detectar uma pluralidade de sequências de ácidos nucleicos alvo na amostra
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US12239993B2 (en) 2018-09-03 2025-03-04 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
KR20220005009A (ko) 2019-04-28 2022-01-12 비스비 메디컬, 인코포레이티드 디지털 검출 능력 및 무선 연결성을 갖는 분자 진단 디바이스
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
US20220340981A1 (en) 2019-09-23 2022-10-27 Universiteit Gent Probe and method for str-genotyping
WO2021138544A1 (en) 2020-01-03 2021-07-08 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术(深圳)有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
US20230068047A1 (en) * 2021-08-19 2023-03-02 Luminex Corporation Digital amplification assay analysis method
EP4541906A1 (en) * 2022-06-20 2025-04-23 Hitachi High-Tech Corporation Primer, dna detection method, and dna detection kit

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
JP3898228B2 (ja) 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
WO1997046714A1 (en) * 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
EP1210358A4 (en) 1999-08-13 2005-01-05 Univ Brandeis DETECTION OF NUCLEIC ACIDS
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
WO2001031062A1 (en) 1999-10-22 2001-05-03 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
EP1436391A4 (en) * 2001-06-08 2005-02-16 Shanghai Mendel Dna Ct Co Ltd CYCLIC EXTENSION AT LOW TEMPERATURE OF DNA WITH HIGH SPECIFICITY OF BEGINNING
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
EP1444365A4 (en) * 2001-10-25 2005-07-20 Gorilla Genomics Inc ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASERESISTENT MOLECULAR BEACONS
AU2002346484A1 (en) 2001-11-21 2003-06-10 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
EP1394271B1 (en) * 2002-09-02 2007-11-14 Toyo Boseki Kabushiki Kaisha Method for identifying nucleotide polymorphisms using resonance energy transfer
WO2004048516A2 (en) * 2002-11-22 2004-06-10 North Shore-Long Island Jewish Research Institute Elf3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
CA2548205C (en) * 2003-12-03 2017-06-27 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
CN107988363A (zh) * 2004-03-31 2018-05-04 综合医院公司 测定癌症对表皮生长因子受体靶向性治疗反应性的方法
KR101424812B1 (ko) * 2004-10-18 2014-08-07 브랜데이스 유니버시티 핵산 증폭을 위한 프라이머, 프로브 및 방법

Similar Documents

Publication Publication Date Title
JP2008516612A5 (enExample)
Jiang et al. G-quadruplex fluorescent probe-mediated real-time rolling circle amplification strategy for highly sensitive microRNA detection
US20070154940A1 (en) Method for detecting target nucleic acid
JP6144623B2 (ja) 核酸測定用の核酸プローブ
WO2006128010A3 (en) Quantification of nucleic acids and proteins using oligonucleotide mass tags
JP2011530296A5 (enExample)
Zhu et al. Label‐Free Detection of MicroRNA: Two‐Step Signal Enhancement with a Hairpin‐Probe‐Based Graphene Fluorescence Switch and Isothermal Amplification
JP2011530296A (ja) Pcrアッセイのための検出アルゴリズム
JP7170062B2 (ja) サンプル内複数のターゲット核酸配列の検出のための方法及び装置
WO2008046645A3 (en) A new method for qualitative and quantitative detection of short nucleic acid sequences of about 8-50 nucleotides in length
WO2004059009A1 (en) A method of real-time detection of nucleic acid sequences
WO2007087336A3 (en) Methods for detecting nucleic acids using multiple signals
RU2007132732A (ru) СПОСОБ КОЛИЧЕСТВЕННОГО АНАЛИЗА МИКРООРГАНИЗМА С ПРИМЕНЕНИЕМ pPHK В КАЧЕСТВЕ МИШЕНИ
EP1375674A1 (en) Method of detecting nucleic acids using probes with fluorescent labels by analysis of the fluorescence melting curves
JP5593582B2 (ja) 核酸の迅速な検出方法
Cheng et al. Ligase chain reaction coupled with rolling circle amplification for high sensitivity detection of single nucleotide polymorphisms
Darius et al. Visual detection of DNA from salmonella and mycobacterium using split DNAzymes
JP2010017127A5 (enExample)
JP2008535478A (ja) シグナル増幅による核酸の検出および分析のための方法および組成物
JP5096007B2 (ja) 核酸プローブセットを用いるリアルタイムpcr方法
Xu et al. Branched RCA coupled with a NESA-based fluorescence assay for ultrasensitive detection of miRNA
JP6720161B2 (ja) 複数の標的核酸の検出キット及びそれを用いる検出方法
KR101899371B1 (ko) 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
JP2009077736A (ja) 核酸の新規測定方法
Feng et al. Label-free optical bifunctional oligonucleotide probe for homogeneous amplification detection of disease markers