JP2001504838A - タンパク質精製 - Google Patents
タンパク質精製Info
- Publication number
- JP2001504838A JP2001504838A JP52465898A JP52465898A JP2001504838A JP 2001504838 A JP2001504838 A JP 2001504838A JP 52465898 A JP52465898 A JP 52465898A JP 52465898 A JP52465898 A JP 52465898A JP 2001504838 A JP2001504838 A JP 2001504838A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- antibody
- antibodies
- solid phase
- immunoglobulin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/828—Protein A
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.プロテインAクロマトグラフィーによる不純化溶液からCH2/CH3領域を含むタ ンパク質を精製する方法であり、 (a)シリカまたはガラスを含む固相に固定化されたプロテインAに該タンパク 質を吸着させ; (b)疎水性電解質溶媒を用いて固相を洗浄することによって固相に結合した不 純物を除去し;及び (c)固相から該タンパク質を回収する ことを含む精製法。 2.該タンパク質が抗体である請求項1の方法。 3.該タンパク質かイムノアドヘシンである請求項1の方法。 4.該疎水性電解質溶媒が塩化テトラメチルアンモニウム(TMAC)を含む請求項1 の方法。 5.該疎水性電解質溶媒が塩化テトラエチルアンモニウム(TEAC)を含む請求項1 の方法。 6.該固相が制御された直径のガラスカラムである請求項1の方法。 7.該固相がケイ酸カラムである請求項1の方法。 8.該不純物がチャイニーズハムスター卵巣タンパク質(CHOP)である請求項1の 方法。 9.疎水性電解質溶媒中の疎水性電解質の濃度が約0.1から約1.0Mの範囲である請 求項1の方法。 10.疎水性電解質溶媒のpHが約4から約8の範囲である請求項1の方法。 11.工程(c)が約2.0から約5.0の範囲のpHを有する溶出バッファーを用いて該タ ンパク質を溶出することを含む請求項1の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3150096P | 1996-11-27 | 1996-11-27 | |
US60/031,500 | 1996-11-27 | ||
PCT/US1997/019994 WO1998023645A1 (en) | 1996-11-27 | 1997-10-29 | Affinity purification of polypeptide on protein a matrix |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001504838A true JP2001504838A (ja) | 2001-04-10 |
JP4031049B2 JP4031049B2 (ja) | 2008-01-09 |
Family
ID=21859810
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52465898A Expired - Lifetime JP4031049B2 (ja) | 1996-11-27 | 1997-10-29 | タンパク質精製 |
Country Status (16)
Country | Link |
---|---|
US (4) | US6127526A (ja) |
EP (4) | EP0950067B1 (ja) |
JP (1) | JP4031049B2 (ja) |
AR (1) | AR010073A1 (ja) |
AT (4) | ATE451391T1 (ja) |
AU (1) | AU729164B2 (ja) |
CA (1) | CA2271255C (ja) |
DE (4) | DE69739673D1 (ja) |
DK (4) | DK1864999T3 (ja) |
ES (4) | ES2335365T3 (ja) |
HK (2) | HK1112248A1 (ja) |
IL (1) | IL130061A (ja) |
PT (4) | PT950067E (ja) |
SI (4) | SI0950067T1 (ja) |
WO (1) | WO1998023645A1 (ja) |
ZA (1) | ZA979996B (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014192877A1 (ja) * | 2013-05-29 | 2014-12-04 | Jsr株式会社 | 洗浄用組成物、タンパク質精製方法、及びタンパク質 |
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WO2014192877A1 (ja) * | 2013-05-29 | 2014-12-04 | Jsr株式会社 | 洗浄用組成物、タンパク質精製方法、及びタンパク質 |
JPWO2014192877A1 (ja) * | 2013-05-29 | 2017-02-23 | Jsr株式会社 | 洗浄用組成物、タンパク質精製方法、及びタンパク質 |
JP2018145437A (ja) * | 2013-05-29 | 2018-09-20 | Jsr株式会社 | 洗浄用組成物、タンパク質精製方法、及びタンパク質 |
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