IL89391A

IL89391A - A structural gene that encodes an insecticidal mutant endotoxin protein, the said protein is produced and the use of the said gene and protein

A structural gene that encodes an insecticidal mutant endotoxin protein, the said protein is produced and the use of the said gene and protein

Info

Publication number: IL89391A
Authority: IL; Israel
Prior art keywords: amino acid; dna; natural amino; acid except; prak
Prior art date: 1988-02-25

Application number

IL8939189A

Other languages

English (en)

Hebrew (he)

Other versions

IL89391A0 (en

Original Assignee

Sandoz Ag

Priority date

1988-02-25

Filing date

1989-02-23

Publication date

1995-01-24

1989-02-23 Application filed by Sandoz Ag filed Critical Sandoz Ag

1989-09-10 Publication of IL89391A0 publication Critical patent/IL89391A0/xx

1995-01-24 Publication of IL89391A publication Critical patent/IL89391A/en

Links

239000002158 endotoxin Substances 0.000 title claims description 138
108090000623 proteins and genes Proteins 0.000 title claims description 84
102000004169 proteins and genes Human genes 0.000 title claims description 39
230000000749 insecticidal effect Effects 0.000 title claims description 15
238000004519 manufacturing process Methods 0.000 title claims description 12
150000001413 amino acids Chemical class 0.000 claims description 118
125000003275 alpha amino acid group Chemical group 0.000 claims description 55
241000588724 Escherichia coli Species 0.000 claims description 38
239000002773 nucleotide Substances 0.000 claims description 37
125000003729 nucleotide group Chemical group 0.000 claims description 37
238000000034 method Methods 0.000 claims description 32
241000238631 Hexapoda Species 0.000 claims description 18
239000000203 mixture Substances 0.000 claims description 18
239000013598 vector Substances 0.000 claims description 15
230000008859 change Effects 0.000 claims description 11
239000013604 expression vector Substances 0.000 claims description 11
230000002588 toxic effect Effects 0.000 claims description 11
238000007792 addition Methods 0.000 claims description 9
230000001580 bacterial effect Effects 0.000 claims description 8
238000012217 deletion Methods 0.000 claims description 8
230000037430 deletion Effects 0.000 claims description 8
238000012986 modification Methods 0.000 claims description 4
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238000012258 culturing Methods 0.000 claims 1
239000013612 plasmid Substances 0.000 description 107
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239000012634 fragment Substances 0.000 description 61
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QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 9
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