HUT77916A - Molekuláris jelölőrendszer - Google Patents

Molekuláris jelölőrendszer

Info

Publication number
HUT77916A
HUT77916A HU9801187A HU9801187A HUT77916A HU T77916 A HUT77916 A HU T77916A HU 9801187 A HU9801187 A HU 9801187A HU 9801187 A HU9801187 A HU 9801187A HU T77916 A HUT77916 A HU T77916A
Authority
HU
Hungary
Prior art keywords
polynucleotides
particles
tagging system
substrate
molecular tagging
Prior art date
Application number
HU9801187A
Other languages
English (en)
Inventor
Sydney Brenner
Original Assignee
Lynx Therapeutics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics, Inc. filed Critical Lynx Therapeutics, Inc.
Publication of HUT77916A publication Critical patent/HUT77916A/hu

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B80/00—Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
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    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00583—Features relative to the processes being carried out
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    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00583—Features relative to the processes being carried out
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    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
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    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Pyrane Compounds (AREA)
  • Magnetic Heads (AREA)
  • Control Of Driving Devices And Active Controlling Of Vehicle (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Air Transport Of Granular Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Multiple-Way Valves (AREA)
  • Non-Silver Salt Photosensitive Materials And Non-Silver Salt Photography (AREA)
  • Fluid-Driven Valves (AREA)
HU9801187A 1994-10-13 1995-10-12 Molekuláris jelölőrendszer HUT77916A (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32234894A 1994-10-13 1994-10-13
US08/358,810 US5604097A (en) 1994-10-13 1994-12-19 Methods for sorting polynucleotides using oligonucleotide tags

Publications (1)

Publication Number Publication Date
HUT77916A true HUT77916A (hu) 1998-10-28

Family

ID=26983370

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9801187A HUT77916A (hu) 1994-10-13 1995-10-12 Molekuláris jelölőrendszer

Country Status (13)

Country Link
US (4) US5604097A (hu)
EP (3) EP1724348A3 (hu)
JP (6) JP4206130B2 (hu)
KR (2) KR970707279A (hu)
AT (1) ATE323159T1 (hu)
AU (2) AU712929B2 (hu)
CA (1) CA2202167C (hu)
CZ (1) CZ86697A3 (hu)
DE (1) DE69534930T2 (hu)
FI (1) FI971473A (hu)
HU (1) HUT77916A (hu)
NO (1) NO971644L (hu)
WO (1) WO1996012014A1 (hu)

Families Citing this family (508)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
AU1248292A (en) 1990-12-06 1992-07-08 Affymax Technologies N.V. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
US6156501A (en) * 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US7375198B2 (en) * 1993-10-26 2008-05-20 Affymetrix, Inc. Modified nucleic acid probes
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6654505B2 (en) 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6280935B1 (en) 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6974666B1 (en) * 1994-10-21 2005-12-13 Appymetric, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
JP4293634B2 (ja) * 1995-06-07 2009-07-08 ソレクサ・インコーポレイテッド 分類および同定のためのオリゴヌクレオチドタグ
EP0832287B1 (en) * 1995-06-07 2007-10-10 Solexa, Inc Oligonucleotide tags for sorting and identification
US5780231A (en) * 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
EP0880598A4 (en) * 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6613508B1 (en) * 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5948615A (en) * 1996-04-16 1999-09-07 Hitachi, Ltd. Method for analysis of nucleic acid and DNA primer sets for use therein
US7041510B2 (en) 1996-04-25 2006-05-09 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6958245B2 (en) 1996-04-25 2005-10-25 Bioarray Solutions Ltd. Array cytometry
DE69737883T2 (de) * 1996-04-25 2008-03-06 Bioarray Solutions Ltd. Licht-regulierte, elektrokinetische zusammensetzung von partikeln an oberflächen
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US7144119B2 (en) * 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6048691A (en) * 1996-05-13 2000-04-11 Motorola, Inc. Method and system for performing a binding assay
AU730633B2 (en) 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
HUP0003944A3 (en) * 1996-06-06 2003-08-28 Lynx Therapeutics Inc Hayward Sequencing by ligation of encoded adaptors
CA2301230A1 (en) 1996-09-20 1998-03-26 Digital Drives, Inc. Spatially addressable combinatorial chemical arrays in cd-rom format
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US6258533B1 (en) * 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
GB9708606D0 (en) * 1997-04-28 1997-06-18 Webster Andrew Sequencing
US6007990A (en) * 1997-04-29 1999-12-28 Levine; Robert A. Detection and quantification of one or more nucleotide sequence target analytes in a sample using spatially localized target analyte replication
JP2002502237A (ja) * 1997-05-12 2002-01-22 ライフ テクノロジーズ，インコーポレイテッド 核酸分子の生成および精製のための方法
US6969488B2 (en) * 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
EP0985142A4 (en) * 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
AU8139698A (en) * 1997-06-05 1998-12-21 Cellstore Methods and reagents for indexing and encoding nucleic acids
US6096496A (en) * 1997-06-19 2000-08-01 Frankel; Robert D. Supports incorporating vertical cavity emitting lasers and tracking apparatus for use in combinatorial synthesis
DE69829058D1 (de) * 1997-06-27 2005-03-24 Lynx Therapeutics Inc Verfahren zur restriktionskarten-analyse von polynukleotiden
WO1999004042A1 (en) * 1997-07-16 1999-01-28 Burden David W Synthetic oligonucleotide-peptide conjugates and methods of use
US6607878B2 (en) 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US6136537A (en) * 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
US6019896A (en) * 1998-03-06 2000-02-01 Molecular Dynamics, Inc. Method for using a quality metric to assess the quality of biochemical separations
IL138287A0 (en) * 1998-03-18 2001-10-31 Quark Biotech Inc Selection subtraction approach to gene identification
WO1999055886A1 (en) * 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
NZ508548A (en) * 1998-05-13 2003-06-30 Spectra Science Corp Micro-lasing beads and structures, and associated methods
DE69940430D1 (de) 1998-06-24 2009-04-02 Illumina Inc Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
JP4651196B2 (ja) * 1998-11-02 2011-03-16 ソレクサ・インコーポレイテッド 相補的オリゴヌクレオチドタグセットを作製するための方法
AU769964B2 (en) * 1998-11-27 2004-02-12 Synaptics (Uk) Limited Position sensor
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
WO2000050632A2 (en) * 1999-02-22 2000-08-31 Lynx Therapeutics, Inc. Polymorphic dna fragments and uses thereof
GB9905807D0 (en) * 1999-03-12 1999-05-05 Amersham Pharm Biotech Uk Ltd Analysis of differential gene expression
WO2000056925A2 (en) * 1999-03-19 2000-09-28 Aclara Biosciences, Inc. Methods for single nucleotide polymorphism detection
CA2366459A1 (en) * 1999-03-26 2000-10-05 Affymetrix, Inc. Universal arrays
AU778438B2 (en) 1999-04-06 2004-12-02 Yale University Fixed address analysis of sequence tags
US20060275782A1 (en) * 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6544732B1 (en) * 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
EP2360270B1 (en) 1999-05-20 2016-11-09 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US8080380B2 (en) * 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20020042081A1 (en) * 2000-10-10 2002-04-11 Eric Henderson Evaluating binding affinities by force stratification and force panning
US20030073250A1 (en) * 1999-05-21 2003-04-17 Eric Henderson Method and apparatus for solid state molecular analysis
US6573369B2 (en) * 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US20030186311A1 (en) * 1999-05-21 2003-10-02 Bioforce Nanosciences, Inc. Parallel analysis of molecular interactions
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6465183B2 (en) 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
CA2383264A1 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
DK1218545T3 (da) 1999-08-18 2012-02-20 Illumina Inc Fremgangsmåder til fremstilling af oligonukleotidopløsninger
WO2001014589A2 (en) * 1999-08-20 2001-03-01 Luminex Corporation Liquid array technology
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7211390B2 (en) * 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
AU7586100A (en) * 1999-09-17 2001-04-17 Affymetrix, Inc. Method of cluster analysis of gene expression profiles
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US7809382B2 (en) * 2000-04-11 2010-10-05 Telecommunication Systems, Inc. Short message distribution center
US6235483B1 (en) * 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
WO2001057269A2 (en) * 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7361488B2 (en) * 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
US7582420B2 (en) * 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) * 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
DE60127939T2 (de) 2000-02-07 2008-01-24 Illumina, Inc., San Diego Nukleinsäure-Nachweisverfahren mit universellem Priming
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
AU2001238389B2 (en) 2000-02-16 2006-09-21 Illumina, Inc. Parallel genotyping of multiple patient samples
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
US6749756B1 (en) * 2000-02-18 2004-06-15 University Of Pittsburgh Reaction and separation methods
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
US7157564B1 (en) 2000-04-06 2007-01-02 Affymetrix, Inc. Tag nucleic acids and probe arrays
US6368801B1 (en) 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
AU2001267191A1 (en) * 2000-06-06 2001-12-17 Tm Bioscience Corporation Capture moieties for nucleic acids and uses thereof
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
EP1311839B1 (en) * 2000-06-21 2006-03-01 Bioarray Solutions Ltd Multianalyte molecular analysis using application-specific random particle arrays
DE60130763T2 (de) 2000-06-30 2008-07-17 Qiagen Gmbh Signalamplifikation mit "lollipop"-hydridisierungsproben
WO2002014553A2 (en) * 2000-08-11 2002-02-21 Favrille, Inc. A molecular vector identification system
US6911204B2 (en) 2000-08-11 2005-06-28 Favrille, Inc. Method and composition for altering a B cell mediated pathology
US6824981B2 (en) * 2000-08-11 2004-11-30 Agilix Corporation Ultra-sensitive detection systems using alterable peptide tags
DE60135150D1 (de) * 2000-08-15 2008-09-11 Bioforce Nanosciences Inc Vorrichtung zur bildung von nanomolekularen netzwerken
GB0021367D0 (en) * 2000-09-01 2000-10-18 Sec Dep Of The Home Department Improvements in and relating to marking
US7057704B2 (en) * 2000-09-17 2006-06-06 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
CA2425747C (en) * 2000-10-14 2012-01-24 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US20030045005A1 (en) * 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
CA2426824A1 (en) 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20040018491A1 (en) * 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US6861222B2 (en) * 2000-11-09 2005-03-01 Yale University Nucleic acid detection using structured probes
JP4516273B2 (ja) * 2000-11-15 2010-08-04 ミナーヴァ・バイオテクノロジーズ・コーポレーション オリゴヌクレオチド識別子
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
US20030049616A1 (en) * 2001-01-08 2003-03-13 Sydney Brenner Enzymatic synthesis of oligonucleotide tags
US6958217B2 (en) * 2001-01-24 2005-10-25 Genomic Expression Aps Single-stranded polynucleotide tags
WO2002059355A2 (en) * 2001-01-25 2002-08-01 Tm Bioscience Corporation Polynucleotides for use as tags and tag complements, manufacture and use thereof
US7226737B2 (en) * 2001-01-25 2007-06-05 Luminex Molecular Diagnostics, Inc. Polynucleotides for use as tags and tag complements, manufacture and use thereof
AU2002231934A1 (en) * 2001-01-30 2002-08-12 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
JP2005522980A (ja) * 2001-04-23 2005-08-04 エリトラ ファーマシューティカルズ，インコーポレイテッド アスペルギルス・フミガーツス（Ａｓｐｅｒｇｉｌｌｕｓｆｕｍｉｇａｔｕｓ）の必須遺伝子の同定および使用方法
EP1262563A3 (en) * 2001-05-31 2003-02-05 Takara Bio Inc. Method for immobilizing DNA on a surface
US20070184436A1 (en) * 2001-06-07 2007-08-09 Joel Myerson Generic capture probe arrays
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
US20060234231A1 (en) * 2001-06-20 2006-10-19 Nuevolution A/S Microarrays displaying encoded molecules
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20030134273A1 (en) * 2001-07-17 2003-07-17 Eric Henderson Combined molecular binding detection through force microscopy and mass spectrometry
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US7026123B1 (en) 2001-08-29 2006-04-11 Pioneer Hi-Bred International, Inc. UTR tag assay for gene function discovery
US7042488B2 (en) 2001-09-27 2006-05-09 Fujinon Corporation Electronic endoscope for highlighting blood vessel
US20030148335A1 (en) * 2001-10-10 2003-08-07 Li Shen Detecting targets by unique identifier nucleotide tags
US6767733B1 (en) 2001-10-10 2004-07-27 Pritest, Inc. Portable biosensor apparatus with controlled flow
JP4377689B2 (ja) 2001-10-15 2009-12-02 バイオアレイ ソリューションズ リミテッド 同時尋問及び酵素仲介検出による多型遺伝子座の複合分析
US7132236B2 (en) * 2001-10-25 2006-11-07 Agilent Technologies, Inc. Composition and method for optimized hybridization using modified solutions
JPWO2003038091A1 (ja) * 2001-10-29 2005-04-07 独立行政法人科学技術振興機構 ミスハイブリダイゼーションを回避しうるオリゴヌクレオチド配列とその設計方法
EP1451588A4 (en) * 2001-11-06 2005-03-09 Agilix Corp SENSITIVE CODED DEFINITION SYSTEMS
EP1314783B1 (de) * 2001-11-22 2008-11-19 Sloning BioTechnology GmbH Nukleinsäure-Linker und deren Verwendung in der Gensynthese
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US20040020993A1 (en) * 2001-12-28 2004-02-05 Green Larry R. Method for luminescent identification and calibration
ATE414769T1 (de) 2002-03-15 2008-12-15 Nuevolution As Eine verbesserte methode zur synthese von matritzenabhängigen molekülen
DE60326224D1 (de) * 2002-04-26 2009-04-02 Solexa Inc Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden
US20040060987A1 (en) * 2002-05-07 2004-04-01 Green Larry R. Digital image analysis method for enhanced and optimized signals in fluorophore detection
US20050239193A1 (en) * 2002-05-30 2005-10-27 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of microorganisms and microparticles
WO2004001074A1 (en) * 2002-06-21 2003-12-31 Lynx Therapeutics, Inc. Method for detecting foreign dna in a host genome
US20050186573A1 (en) * 2002-07-24 2005-08-25 Janeczko Richard A. Polynucleotides for use as tags and tag complements in the detection of nucleic acid sequences
AU2003247266A1 (en) * 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
DK3363809T3 (da) 2002-08-23 2020-05-04 Illumina Cambridge Ltd Modificerede nukleotider til polynukleotidsekvensering
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US20040043384A1 (en) * 2002-08-28 2004-03-04 Oleinikov Andrew V. In vitro protein translation microarray device
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
AU2003266932B2 (en) * 2002-09-27 2009-04-09 Mpm-Holding Aps Spatially encoded polymer matrix
US20040259105A1 (en) * 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
ES2640279T3 (es) 2002-10-30 2017-11-02 Nuevolution A/S Procedimiento para la síntesis de un complejo bifuncional
US20040086892A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
EP1756277B1 (en) 2002-12-19 2009-12-02 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
KR20050100367A (ko) * 2003-01-02 2005-10-18 바이오포스 나노사이언스, 인크. 소용적 샘플 내의 분자 분석용 방법 및 장치
US7514212B2 (en) * 2003-01-17 2009-04-07 Eragen Biosciences, Inc. Nucleic acid amplification using non-standard bases
CA2513985C (en) * 2003-01-21 2012-05-29 Illumina Inc. Chemical reaction monitor
WO2004069849A2 (en) * 2003-01-29 2004-08-19 454 Corporation Bead emulsion nucleic acid amplification
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20060228786A1 (en) * 2003-02-06 2006-10-12 Salerno John C Polymerase-based protocols for the introduction of deletions and insertions
US20060234238A1 (en) * 2003-02-06 2006-10-19 Salerno John C Polymerase-based protocols for generating chimeric oligonucleotides
US20060257876A1 (en) * 2003-02-06 2006-11-16 Rensselaer Polytechnis Institute Polymerase-based protocols for generating chimeric and combinatorial...
US20060134624A1 (en) * 2003-02-06 2006-06-22 Salerno John C Polymerase-based protocols for the introductions of deletions and insertions
US20070026397A1 (en) * 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
EP1597394A2 (en) * 2003-02-21 2005-11-23 Nuevolution A/S A method for obtaining structural information about an encoded molecule
EP2236606B1 (en) 2003-03-20 2013-11-06 Nuevolution A/S Ligational encoding of small molecules
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20060078893A1 (en) * 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
JP4567673B2 (ja) 2003-04-01 2010-10-20 エラジェン バイオサイエンシズ インコーポレイテッド ポリメラーゼ阻害剤およびその使用方法
US7195875B2 (en) * 2003-04-18 2007-03-27 Beckman Coulter, Inc. Oligonucleotide pairs for multiplexed binding assays
JP2004355294A (ja) * 2003-05-29 2004-12-16 National Institute Of Advanced Industrial & Technology 情報担体としてのｄｎａ符号の設計方法
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis
WO2005010494A2 (en) * 2003-07-21 2005-02-03 Jie Wu Multiplexed analyte detection
WO2005026686A2 (en) * 2003-09-09 2005-03-24 Compass Genetics, Llc Multiplexed analytical platform
US7927796B2 (en) * 2003-09-18 2011-04-19 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
EP1670939B1 (en) * 2003-09-18 2009-11-04 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
US20050100939A1 (en) * 2003-09-18 2005-05-12 Eugeni Namsaraev System and methods for enhancing signal-to-noise ratios of microarray-based measurements
US7595279B2 (en) * 2003-09-22 2009-09-29 Bioarray Solutions Ltd. Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US20050089916A1 (en) * 2003-10-28 2005-04-28 Xiongwu Xia Allele assignment and probe selection in multiplexed assays of polymorphic targets
CA2544041C (en) * 2003-10-28 2015-12-08 Bioarray Solutions Ltd. Optimization of gene expression analysis using immobilized capture probes
PT1694859E (pt) 2003-10-29 2015-04-13 Bioarray Solutions Ltd Análise de ácidos nucleicos multiplexada através de fragmentação de adn de cadeia dupla
US20050094807A1 (en) * 2003-11-04 2005-05-05 John Silzel Accuracy array assay system and method
DE602004025371D1 (de) * 2003-11-10 2010-03-18 Geneohm Sciences Inc Nukleinsäurenachweisverfahren mit erhöhter empfindlichkeit
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP3175914A1 (en) 2004-01-07 2017-06-07 Illumina Cambridge Limited Improvements in or relating to molecular arrays
EP1709203A2 (en) * 2004-01-23 2006-10-11 Lingvitae AS Improving polynucleotide ligation reactions
ATE483020T1 (de) 2004-01-23 2010-10-15 Sloning Biotechnology Gmbh Enzymatische herstellung von nukleinsäuremolekülen
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
GB0402895D0 (en) * 2004-02-10 2004-03-17 Solexa Ltd Arrayed polynucleotides
US20080108515A1 (en) * 2004-02-10 2008-05-08 Gormley Niall A Arrayed polynucleotides
AU2005214329A1 (en) * 2004-02-12 2005-09-01 Population Genetics Technologies Ltd Genetic analysis by sequence-specific sorting
EP1723255B1 (en) * 2004-02-17 2010-12-29 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
EP1730277B1 (en) 2004-03-22 2009-10-28 Nuevolution A/S Ligational encoding using building block oligonucleotides
EP1582599A1 (en) * 2004-03-31 2005-10-05 Takara Bio Inc. Method for purifying microbeads
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US20050266447A1 (en) * 2004-04-19 2005-12-01 Pioneer Hi-Bred International, Inc. Method for identifying activators of gene transcription
US20050250147A1 (en) * 2004-05-10 2005-11-10 Macevicz Stephen C Digital profiling of polynucleotide populations
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP2065466B1 (en) 2004-05-28 2014-07-09 Asuragen, Inc. Methods and compositions involving MicroRNA
US7363170B2 (en) * 2004-07-09 2008-04-22 Bio Array Solutions Ltd. Transfusion registry network providing real-time interaction between users and providers of genetically characterized blood products
US20060019304A1 (en) * 2004-07-26 2006-01-26 Paul Hardenbol Simultaneous analysis of multiple genomes
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP1623996A1 (en) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved method of selecting a desired protein from a library
ATE494392T1 (de) 2004-09-21 2011-01-15 Life Technologies Corp Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060099626A1 (en) * 2004-10-27 2006-05-11 Harbury Pehr B DNA-templated combinatorial library device and method for use
EP2281889B1 (en) 2004-11-12 2014-07-30 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
DE502005003509D1 (de) * 2004-11-19 2008-05-08 Ebm Papst St Georgen Gmbh & Co Anordnung mit einem luefter und einer pumpe
US20080003571A1 (en) 2005-02-01 2008-01-03 Mckernan Kevin Reagents, methods, and libraries for bead-based sequencing
EP2241637A1 (en) 2005-02-01 2010-10-20 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
CA2596117A1 (en) * 2005-02-03 2006-08-10 Perkinelmer Las, Inc. Ultra-sensitive detection systems using multidimension signals
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060204971A1 (en) * 2005-03-11 2006-09-14 Varde Shobha A Oligonucleotides for multiplexed binding assays
US20060211030A1 (en) * 2005-03-16 2006-09-21 Sydney Brenner Methods and compositions for assay readouts on multiple analytical platforms
US7498136B2 (en) * 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
EP1893777B1 (en) 2005-06-07 2014-10-01 Luminex Corporation Methods for detection and typing of nucleic acids
EP2463386B1 (en) 2005-06-15 2017-04-12 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US20090117552A1 (en) * 2005-07-07 2009-05-07 Pamgene Bv Method for Detection and Quantification of Target Nucleic Acids in a Sample
US20070172429A1 (en) * 2005-08-12 2007-07-26 Xiaolian Gao Labeling compositions and methods of use for deterrent trackability
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP1957644B1 (en) 2005-12-01 2010-12-01 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
CA2636855C (en) 2006-01-11 2016-09-27 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007087312A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
EP1987162A4 (en) * 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
EP2007907A2 (en) * 2006-04-19 2008-12-31 Applera Corporation Reagents, methods, and libraries for gel-free bead-based sequencing
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
US7923533B2 (en) * 2006-06-29 2011-04-12 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US20080004813A1 (en) * 2006-06-29 2008-01-03 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Systems for arbitrary peptide synthesis
US7777002B2 (en) * 2006-06-29 2010-08-17 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7910695B2 (en) * 2006-06-29 2011-03-22 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US7879975B2 (en) * 2006-06-29 2011-02-01 The Invention Science Fund I, Llc Methods for arbitrary peptide synthesis
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US20090002703A1 (en) * 2006-08-16 2009-01-01 Craig Edward Parman Methods and systems for quantifying isobaric labels and peptides
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
AU2007299828C1 (en) 2006-09-19 2014-07-17 Interpace Diagnostics, Llc MicroRNAs differentially expressed in pancreatic diseases and uses thereof
JP2010510964A (ja) 2006-09-19 2010-04-08 アシュラジェン インコーポレイテッド 治療的介入の標的としての、ｍｉＲ−１５、ｍｉＲ−２６、ｍｉＲ−３１、ｍｉＲ−１４５、ｍｉＲ−１４７、ｍｉＲ−１８８、ｍｉＲ−２１５、ｍｉＲ−２１６、ｍｉＲ−３３１、ｍｍｕ−ｍｉＲ−２９２−３ｐによって調節される遺伝子および経路
US7955802B2 (en) 2006-12-13 2011-06-07 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CL2008001682A1 (es) 2007-06-08 2008-12-12 Monsanto Technology Llc Metodos para el mejoramiento de plantas mediante el uso de informacion directa de secuencias de acidos nucleicos.
WO2009031054A2 (en) * 2007-06-29 2009-03-12 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
WO2009040682A2 (en) * 2007-09-26 2009-04-02 Population Genetics Technologies Ltd. Methods and compositions for reducing the complexity of a nucleic acid sample
CA2704487A1 (en) * 2007-11-02 2009-06-18 Luminex Molecular Diagnostics, Inc. One-step target detection assay
WO2009059199A2 (en) * 2007-11-02 2009-05-07 Hunch Inc. Interactive machine learning advice facility
US8815576B2 (en) * 2007-12-27 2014-08-26 Lawrence Livermore National Security, Llc. Chip-based sequencing nucleic acids
US9115352B2 (en) 2008-03-31 2015-08-25 Sloning Biotechnology Gmbh Method for the preparation of a nucleic acid library
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
EP2990487A1 (en) 2008-05-08 2016-03-02 Asuragen, INC. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
EP2291533B2 (en) * 2008-07-02 2020-09-30 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
EP2318552B1 (en) 2008-09-05 2016-11-23 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
GB2467704B (en) 2008-11-07 2011-08-24 Mlc Dx Inc A method for determining a profile of recombined DNA sequences in T-cells and/or B-cells
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8685898B2 (en) 2009-01-15 2014-04-01 Imdaptive, Inc. Adaptive immunity profiling and methods for generation of monoclonal antibodies
SG173627A1 (en) 2009-02-13 2011-09-29 Chem Inc X Methods of creating and screening dna-encoded libraries
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
WO2010115100A1 (en) * 2009-04-03 2010-10-07 L&C Diagment, Inc. Multiplex nucleic acid detection methods and systems
CA2760439A1 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
CA2766351C (en) 2009-06-29 2018-02-27 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
WO2011037990A1 (en) 2009-09-22 2011-03-31 President And Fellows Of Harvard College Entangled mate sequencing
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
CN102639712A (zh) * 2009-10-23 2012-08-15 卢米耐克斯公司 用于提高反应特异性的具有非标准碱基的扩增引物
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP3214174B1 (en) 2010-03-04 2019-10-16 InteRNA Technologies B.V. A mirna molecule defined by its source and its diagnostic and therapeutic uses in diseases or conditions associated with emt
WO2011127933A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
US8278049B2 (en) * 2010-04-26 2012-10-02 Ann & Robert H. Lurie Children's Hospital of Chicago Selective enrichment of CpG islands
WO2011139371A1 (en) 2010-05-06 2011-11-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP3369817A1 (en) 2010-07-06 2018-09-05 InteRNA Technologies B.V. Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma , or in diseases or conditions with activated braf pathway
EP3115468B1 (en) 2010-09-21 2018-07-25 Agilent Technologies, Inc. Increasing confidence of allele calls with molecular counting
MX346956B (es) 2010-09-24 2017-04-06 Univ Leland Stanford Junior Captura directa, amplificación y secuenciación de objetivo adn usando cebadores inmovilizados.
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
EP2622103B2 (en) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP6118725B2 (ja) 2010-11-12 2017-04-19 ジェン９・インコーポレイテッドＧｅｎ９，ＩＮＣ． 核酸合成のための方法およびデバイス
CA2817882C (en) 2010-11-17 2022-08-23 Asuragen, Inc. Mirnas as biomarkers for distinguishing benign from malignant thyroid neoplasms
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
CA2828532A1 (en) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Anti-mullerian hormone changes in pregnancy and prediction of adverse pregnancy outcomes and gender
US10501779B2 (en) 2011-05-12 2019-12-10 President And Fellows Of Harvard College Oligonucleotide trapping
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
CN103732744A (zh) * 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
LT3594340T (lt) 2011-08-26 2021-10-25 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
BR112014005205A2 (pt) 2011-09-07 2017-03-21 X-Chem Inc métodos para etiquetar bibliotecas codificadas com dna
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
KR20140066782A (ko) 2011-09-29 2014-06-02 루미넥스 코포레이션 가수분해 프로브
US9228233B2 (en) 2011-10-17 2016-01-05 Good Start Genetics, Inc. Analysis methods
CA2853088C (en) 2011-10-21 2018-03-13 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US20130157884A1 (en) 2011-10-26 2013-06-20 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
WO2013063544A1 (en) 2011-10-27 2013-05-02 Asuragen, Inc. Mirnas as diagnostic biomarkers to distinguish benign from malignant thyroid tumors
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP3211100A1 (en) 2011-12-22 2017-08-30 Ibis Biosciences, Inc. Amplification primers and methods
EP3309262B1 (en) 2012-02-24 2019-09-25 Bio-Rad Laboratories, Inc. Labeling and sample preparation for sequencing
EP2820158B1 (en) 2012-02-27 2018-01-10 Cellular Research, Inc. Compositions and kits for molecular counting
EP2820174B1 (en) 2012-02-27 2019-12-25 The University of North Carolina at Chapel Hill Methods and uses for molecular tags
ES2741099T3 (es) 2012-02-28 2020-02-10 Agilent Technologies Inc Método de fijación de una secuencia de recuento para una muestra de ácido nucleico
EP2823060B1 (en) 2012-03-05 2018-02-14 Adaptive Biotechnologies Corporation Determining paired immune receptor chains from frequency matched subunits
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
EP2844768B1 (en) 2012-04-30 2019-03-13 Raindance Technologies, Inc. Digital analyte analysis
WO2013169957A1 (en) 2012-05-08 2013-11-14 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
WO2013188756A1 (en) 2012-06-15 2013-12-19 The University Of Chicago Oligonucleotide-mediated quantitative multiplexed immunoassays
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
EP2870263A1 (en) 2012-07-03 2015-05-13 InteRNA Technologies B.V. Diagnostic portfolio and its uses
IL236633B (en) 2012-07-13 2022-07-01 X Chem Inc DNA-encoded libraries containing a conjugate containing conjugated oligonucleotide links
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102028375B1 (ko) 2012-09-04 2019-10-04 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
ES2660027T3 (es) 2012-10-01 2018-03-20 Adaptive Biotechnologies Corporation Evaluación de la inmunocompetencia por la diversidad de los receptores de inmunidad adaptativa y caracterización de la clonalidad
WO2014055117A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
US9365896B2 (en) 2012-10-19 2016-06-14 Agilent Technologies, Inc. Addition of an adaptor by invasive cleavage
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
US10597650B2 (en) 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US10597710B2 (en) 2012-12-21 2020-03-24 New England Biolabs, Inc. Ligase activity
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
CA2907377A1 (en) 2013-03-15 2014-09-18 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
KR102230831B1 (ko) 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
US9540685B2 (en) 2013-03-15 2017-01-10 President And Fellows Of Harvard College Methods of identifying homologous genes using FISH
US9944992B2 (en) 2013-03-15 2018-04-17 The University Of Chicago Methods and compositions related to T-cell activity
EP2971149B1 (en) 2013-03-15 2018-05-09 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
US20160068894A1 (en) 2013-04-04 2016-03-10 Georgia State University Research Foundation, Inc. RNA Microchip Detection Using Nanoparticle-Assisted Signal Amplification
EP2986762B1 (en) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP2999792B1 (en) 2013-05-23 2018-11-14 The Board of Trustees of The Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
WO2014197377A2 (en) 2013-06-03 2014-12-11 Good Start Genetics, Inc. Methods and systems for storing sequence read data
WO2014205083A1 (en) 2013-06-19 2014-12-24 Luminex Corporation Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
KR102398399B1 (ko) 2013-08-09 2022-05-16 루미넥스 코포레이션 핵산 에세이에서의 향상된 용융 식별 및 복합화를 위한 프로브
CA2921619C (en) 2013-08-19 2021-05-25 Abbott Molecular Inc. Nucleotide analogs
EP3626866B1 (en) 2013-08-19 2021-03-24 Abbott Molecular Inc. Next-generation sequencing libraries
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015057565A1 (en) 2013-10-18 2015-04-23 Good Start Genetics, Inc. Methods for assessing a genomic region of a subject
CN106170558B (zh) 2013-10-21 2021-10-29 金圣千 利用寡核苷酸的生物分子的分析方法及装置
EP3062252A4 (en) 2013-10-22 2017-06-07 KIM, Sung-Chun Marker for generating binding information on biomolecules and nucleic acids, preparation method therefor, and method and apparatus for analyzing biomolecule by using same
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
EP3378952B1 (en) 2013-12-28 2020-02-05 Guardant Health, Inc. Methods and systems for detecting genetic variants
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
WO2015105179A1 (ja) 2014-01-10 2015-07-16 国立大学法人京都大学 タンパク質と高次構造を含むｒｎａとの相互作用を検出するためのｒｎａマイクロアレイ
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
EP3132059B1 (en) 2014-04-17 2020-01-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
JP6664381B2 (ja) 2014-08-11 2020-03-13 ルミネックス コーポレーション 核酸アッセイにおける改善された融解識別と多重化のためのプローブ
US10093967B2 (en) 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
US10174383B2 (en) 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample
WO2016028843A2 (en) 2014-08-19 2016-02-25 President And Fellows Of Harvard College Rna-guided systems for probing and mapping of nucleic acids
US10648031B2 (en) 2014-09-05 2020-05-12 Qiagen Gmbh Preparation of adapter-ligated amplicons
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
JP2017536087A (ja) 2014-09-24 2017-12-07 グッド スタート ジェネティクス， インコーポレイテッド 遺伝子アッセイのロバストネスを増大させるためのプロセス制御
US10040048B1 (en) 2014-09-25 2018-08-07 Synthego Corporation Automated modular system and method for production of biopolymers
CA3002133A1 (en) 2014-10-17 2016-04-21 Good Start Genetics, Inc. Pre-implantation genetic screening and aneuploidy detection
ES2784343T3 (es) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Detección simultánea altamente multiplexada de ácidos nucleicos que codifican heterodímeros de receptores inmunes adaptativos emparejados de muchas muestras
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
WO2016086029A1 (en) 2014-11-25 2016-06-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
EP3271480B8 (en) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Screening for structural variants
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
US10246702B1 (en) 2015-02-23 2019-04-02 New England Biolabs, Inc. Compositions and methods for labeling target nucleic acid molecules
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016161273A1 (en) 2015-04-01 2016-10-06 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US10508300B2 (en) 2015-09-18 2019-12-17 Vanadis Diagnostics Probe set for analyzing a DNA sample and method for using the same
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
US9931638B2 (en) 2016-02-24 2018-04-03 Latonya Stewart Garbage disposal cleaning system
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017168329A1 (en) 2016-03-28 2017-10-05 Boreal Genomics, Inc. Droplet-based linked-fragment sequencing
US10822643B2 (en) 2016-05-02 2020-11-03 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10240196B2 (en) 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3500669A4 (en) 2016-08-16 2020-01-22 The Regents of the University of California METHOD FOR FINDING LOW FREQUENCY SEQUENCES BY HYBRIDIZATION (FLASH)
EP3885448B1 (en) 2016-09-02 2023-03-22 New England Biolabs, Inc. Analysis of chromatin using a nicking enzyme
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018057928A1 (en) 2016-09-23 2018-03-29 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
US10822651B2 (en) 2016-10-28 2020-11-03 Grail, Inc. Methods for single-stranded nucleic acid library preparation
WO2018089377A1 (en) 2016-11-08 2018-05-17 Cellular Research, Inc. Methods for cell label classification
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス， インコーポレイテッド 連結型ライゲーション
EP4357455A3 (en) 2016-12-12 2024-07-24 Grail, LLC Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
EP3559255A1 (en) 2016-12-23 2019-10-30 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
JP7104048B2 (ja) 2017-01-13 2022-07-20 セルラー リサーチ， インコーポレイテッド 流体チャネルの親水性コーティング
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
CA3062708A1 (en) * 2017-05-23 2018-11-29 Rutgers, The State University Of New Jersey Target mediated in situ signal amplification with dual interacting hairpin probes
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
US11078482B2 (en) 2017-06-15 2021-08-03 Genome Research Limited Duplex sequencing using direct repeat molecules
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
CA3079524A1 (en) 2017-11-03 2019-05-09 Interna Technologies B.V. Mirna molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
WO2019118925A1 (en) 2017-12-15 2019-06-20 Grail, Inc. Methods for enriching for duplex reads in sequencing and error correction
CN111492068A (zh) 2017-12-19 2020-08-04 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
US20190237161A1 (en) 2017-12-22 2019-08-01 Grail, Inc. Error removal using improved library preparation methods
CA3044782A1 (en) 2017-12-29 2019-06-29 Clear Labs, Inc. Automated priming and library loading device
EP3553182A1 (en) 2018-04-11 2019-10-16 Université de Bourgogne Detection method of somatic genetic anomalies, combination of capture probes and kit of detection
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
KR102209178B1 (ko) * 2018-07-17 2021-01-29 이윤경 유전체 및 유전체 정보의 보존 및 활용을 위한 방법
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
EP3894593B1 (en) 2018-12-13 2024-10-02 DNA Script Direct oligonucleotide synthesis on cdna
EP3884071A4 (en) 2019-01-03 2022-04-06 Boreal Genomics, Inc. LINKED TARGET CAPTURE
JP7491934B2 (ja) 2019-01-03 2024-05-28 ディーエヌエー スクリプト オリゴヌクレオチドセットのワンポット合成
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
CN113454234A (zh) 2019-02-14 2021-09-28 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US11512346B2 (en) 2019-03-14 2022-11-29 Genome Research Limited Method for sequencing a direct repeat
KR20210151944A (ko) 2019-04-12 2021-12-14 더 리전트 오브 더 유니버시티 오브 캘리포니아 근육량 및 산화 대사를 증가시키기 위한 조성물 및 방법
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US20220033810A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022029688A1 (en) 2020-08-05 2022-02-10 Inivata Ltd. Highly sensitive method for detecting cancer dna in a sample
WO2023012521A1 (en) 2021-08-05 2023-02-09 Inivata Limited Highly sensitive method for detecting cancer dna in a sample
CN116635533A (zh) 2020-11-20 2023-08-22 贝克顿迪金森公司 高表达的蛋白和低表达的蛋白的谱分析
EP4413582A1 (en) 2021-10-04 2024-08-14 F. Hoffmann-La Roche AG Online base call compression
WO2024003332A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
WO2024028794A1 (en) 2022-08-02 2024-02-08 Temple Therapeutics BV Methods for treating endometrial and ovarian hyperproliferative disorders

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4046720A (en) 1974-01-17 1977-09-06 California Institute Of Technology Crosslinked, porous, polyacrylate beads
US4318846A (en) 1979-09-07 1982-03-09 Syva Company Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4973679A (en) 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4678814A (en) 1981-03-30 1987-07-07 California Institute Of Technology Polyacrolein microspheres
US4413070A (en) 1981-03-30 1983-11-01 California Institute Of Technology Polyacrolein microspheres
US4948882A (en) 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US5206143A (en) 1985-11-01 1993-04-27 Smithkline Beecham Corporation Method and reagents for performing subset analysis using quantitative differences in fluorescence intensity
US4659774A (en) 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
US5093232A (en) 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
US4855225A (en) 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5091519A (en) 1986-05-01 1992-02-25 Amoco Corporation Nucleotide compositions with linking groups
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
SE458968B (sv) 1987-06-16 1989-05-22 Wallac Oy Biospecifikt analysfoerfarande foer flera analyter i vilket ingaar partikelraekning och maerkning med fluorescerande maerksubstanser
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
WO1989003849A1 (en) 1987-10-28 1989-05-05 Howard Florey Institute Of Experimental Physiology Oligonucleotide-polyamide conjugates
US5104791A (en) 1988-02-09 1992-04-14 E. I. Du Pont De Nemours And Company Particle counting nucleic acid hybridization assays
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US5002867A (en) 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
JPH05501709A (ja) 1989-11-17 1993-04-02 リンクス　セラピューティクス，インコーポレイテッド ポリ（アルキルおよびアルケニルホスフェート）およびそれらのチオホスフェートおよび抗ウイルス剤としてのセレノホスフェート誘導体
ATE137269T1 (de) 1990-01-26 1996-05-15 Abbott Lab Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5650489A (en) * 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
DE69130647T2 (de) * 1990-08-24 1999-05-06 Ixsys, Inc., San Diego, Calif. Verfahren zur herstellung von oligonukleotiden mit regellosen codonen
DE69120821T2 (de) 1990-08-31 1997-01-23 The Regents Of The University Of Minnesota, Minneapolis, Minn. Polyethylenglykol-Derivate für Festphasenanwendungen
AU1248292A (en) * 1990-12-06 1992-07-08 Affymax Technologies N.V. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
JPH057490A (ja) * 1991-05-25 1993-01-19 Sumitomo Electric Ind Ltd Ｄｎａ断片の分取法
ATE171543T1 (de) 1991-07-16 1998-10-15 Transmed Biotech Inc Verfahren und zusammensetzungen für die gleichzeitige analyse einer vielzahl von analyten
WO1993005183A1 (en) * 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
EP0604552B1 (en) * 1991-09-18 1997-02-12 Affymax Technologies N.V. Method of synthesizing diverse collections of oligomers
AU2657392A (en) 1991-09-27 1993-04-27 Allelix Biopharmaceuticals Inc. Duplex-forming, polynucleotide conjugates
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
KR100249683B1 (ko) * 1991-12-24 2000-04-01 스티브 민터 핵산서열조작
ATE278807T1 (de) * 1992-02-19 2004-10-15 New York Health Res Inst Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren
CA2133554C (en) * 1992-04-15 2009-07-14 David R. Shortle Synthesis of diverse and useful collections of oligonucleotides
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5541061A (en) * 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
JP3311752B2 (ja) 1992-07-02 2002-08-05 ソイニ，エルッキ 生体特異的多変数検定法
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
WO1994002515A1 (en) * 1992-07-21 1994-02-03 Bunsen Rush Laboratories Inc. Oligomer library formats and methods relating thereto
ES2204910T3 (es) * 1992-10-01 2004-05-01 The Trustees Of Columbia University In The City Of New York Bibliotecas quimicas combinatorias complejas codificadas con señales.
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
EP0743989B1 (en) 1994-02-14 2007-03-21 Smithkline Beecham Corporation Methed of identifying differentially expressed genes
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage

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