ATE494392T1 - Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) - Google Patents

Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)

Info

Publication number
ATE494392T1
ATE494392T1 AT05810351T AT05810351T ATE494392T1 AT E494392 T1 ATE494392 T1 AT E494392T1 AT 05810351 T AT05810351 T AT 05810351T AT 05810351 T AT05810351 T AT 05810351T AT E494392 T1 ATE494392 T1 AT E494392T1
Authority
AT
Austria
Prior art keywords
mirnas
target polynucleotide
rnas
micro
time
Prior art date
Application number
AT05810351T
Other languages
English (en)
Inventor
Andrew Finn
Caifu Chen
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Application granted granted Critical
Publication of ATE494392T1 publication Critical patent/ATE494392T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05810351T 2004-09-21 2005-09-21 Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas) ATE494392T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61219904P 2004-09-21 2004-09-21
PCT/US2005/033943 WO2006034387A1 (en) 2004-09-21 2005-09-21 TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)

Publications (1)

Publication Number Publication Date
ATE494392T1 true ATE494392T1 (de) 2011-01-15

Family

ID=35645577

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05810351T ATE494392T1 (de) 2004-09-21 2005-09-21 Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)

Country Status (6)

Country Link
US (3) US7642055B2 (de)
EP (2) EP2186908A1 (de)
JP (1) JP2008513028A (de)
AT (1) ATE494392T1 (de)
DE (1) DE602005025782D1 (de)
WO (1) WO2006034387A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US7642055B2 (en) 2004-09-21 2010-01-05 Applied Biosystems, Llc Two-color real-time/end-point quantitation of microRNAs (miRNAs)
US20070077570A1 (en) * 2005-05-31 2007-04-05 Applera Corporation Multiplexed amplification of short nucleic acids
WO2007103808A2 (en) 2006-03-02 2007-09-13 The Ohio State University Microrna expression profile associated with pancreatic cancer
US20080038727A1 (en) * 2006-03-10 2008-02-14 Applera Corporation MicroRNA and Messenger RNA Detection on Arrays
US7727745B2 (en) * 2006-08-14 2010-06-01 Bio-Id Diagnostic Inc. Synthesis of single-stranded DNA
US20080161197A1 (en) * 2006-12-12 2008-07-03 Kai Qin Lao Method for amplifying monomorphic-tailed nucleic acids
US20090023190A1 (en) 2007-06-20 2009-01-22 Kai Qin Lao Sequence amplification with loopable primers
EP2548962B1 (de) 2007-09-19 2016-01-13 Applied Biosystems, LLC Sirna-sequenz-unabhängige Modifikationsformate zur Verringerung von das Ziel verfehlenden phänotypischen Effekten bei RNAI und stabilisierte Formen davon
US20090291475A1 (en) 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
DE102012215925B3 (de) * 2012-09-07 2013-09-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Zeitgleicher Nachweis verschiedener microRNA-Biogenese-Formen
JP6577660B2 (ja) 2016-03-18 2019-09-18 株式会社東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
JP2022513408A (ja) 2018-10-31 2022-02-07 ザイマージェン インコーポレイテッド Ｄｎａライブラリーの多重化決定論的アセンブリー

Family Cites Families (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040166A (en) * 1985-03-28 2000-03-21 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences, including a probe
US6197563B1 (en) * 1985-03-28 2001-03-06 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DK0540693T3 (da) 1990-07-24 1999-09-13 Hoffmann La Roche Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
AU5871094A (en) * 1992-12-09 1994-07-04 Jeffrey E. Miller Methods and compositions for cdna synthesis
US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US5876927A (en) * 1993-07-13 1999-03-02 The Regents Of The University Of California Nucleic acid diagnostic assay for Charcot-Marie-Tooth Disease Type 1B
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6821727B1 (en) * 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
EP0706649B1 (de) 1994-04-29 2001-01-03 Perkin-Elmer Corporation Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
HUP9900910A2 (hu) 1995-06-07 1999-07-28 Lynx Therapeutics, Inc. Oligonukleotid jelzések osztályozáshoz és azonosításhoz
US6153425A (en) 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
CA2244891C (en) 1996-02-09 2008-12-30 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6458530B1 (en) 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5982405A (en) 1996-04-18 1999-11-09 Japan Servo Co., Ltd. Thermal transfer recording apparatus and transfer ribbon
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
JP4468488B2 (ja) 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
US5736333A (en) 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US6174670B1 (en) 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
CA2744096C (en) * 1996-07-31 2013-07-30 Laboratory Corporation Of America Holdings Biomarkers and targets for diagnosis, prognosis and management of prostate disease
WO1998015644A2 (en) 1996-09-27 1998-04-16 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method
US6090557A (en) * 1997-04-18 2000-07-18 Roche Molecular Systems, Inc. Neisseria gonorrhoeae-specific oligonucleotides
US6309829B1 (en) 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
EP1025120B1 (de) 1997-10-27 2010-08-18 Boston Probes, Inc. Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6582936B1 (en) * 1997-12-12 2003-06-24 The Regents Of The University Of California Methods for making nucleic acids
US6114152A (en) * 1997-12-12 2000-09-05 The Regents Of The University Of California Methods for making nucleic acids
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
DE69942444D1 (de) * 1998-02-04 2010-07-15 Life Technologies Corp Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6451525B1 (en) 1998-12-03 2002-09-17 Pe Corporation (Ny) Parallel sequencing method
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6383752B1 (en) 1999-03-31 2002-05-07 Hybridon, Inc. Pseudo-cyclic oligonucleobases
WO2000079009A2 (en) * 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
CA2383264A1 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US6528254B1 (en) 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
WO2001092579A2 (en) 2000-05-30 2001-12-06 Pe Corporation (Ny) Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6596490B2 (en) 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6358679B1 (en) * 2000-08-24 2002-03-19 Pe Corporation (Ny) Methods for external controls for nucleic acid amplification
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
US6777180B1 (en) * 2000-11-28 2004-08-17 Trustees Of Columbia University In The City Of New York Method for full-length cDNA cloning using degenerate stem-loop annealing primers
WO2002061143A2 (en) 2001-01-31 2002-08-08 Ambion, Inc. Comparative analysis of nucleic acids using population tagging
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6589250B2 (en) 2001-11-20 2003-07-08 Stephen A. Schendel Maxillary distraction device
EP1540017A4 (de) * 2002-05-09 2006-05-24 Us Genomics Inc Verfahren zur analyse einer nukleinsäure
AU2003259017A1 (en) * 2002-09-06 2004-03-29 Johns Hopkins Singapore Pte Ltd Strand specific detection and quantification
EP1540003A4 (de) * 2002-09-19 2006-06-21 Applera Corp Verfahren und zusammensetzung zum nachweis von targets
JP2006500023A (ja) * 2002-09-20 2006-01-05 ファルマシア・アンド・アップジョン・カンパニー・エルエルシー ランダムＲＮＡｉライブラリーの生成法、および細胞に基づくスクリーニングにおける該ライブラリーの適用
US20040175732A1 (en) * 2002-11-15 2004-09-09 Rana Tariq M. Identification of micrornas and their targets
WO2004046344A2 (en) 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays
CN1836050A (zh) * 2003-07-07 2006-09-20 单细胞系统公司 发夹标记的探针及其使用方法
WO2005047547A1 (en) * 2003-11-04 2005-05-26 Applera Corporation Concatameric ligation products: compositions, methods and kits for same
ATE431433T1 (de) * 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
US7642055B2 (en) 2004-09-21 2010-01-05 Applied Biosystems, Llc Two-color real-time/end-point quantitation of microRNAs (miRNAs)
US7169561B2 (en) * 2004-12-29 2007-01-30 Applera Corporation Methods, compositions, and kits for forming self-complementary polynucleotides
US20070015176A1 (en) * 2005-02-18 2007-01-18 Applera Corporation Small nucleic acid detection probes and uses thereof
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas
ATE532878T1 (de) * 2005-08-24 2011-11-15 Life Technologies Corp Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
FR2989691B1 (fr) 2012-04-24 2014-05-23 Commissariat Energie Atomique Reacteur pour le depot de couche atomique (ald), application a l'encapsulage d'un dispositif oled par depot de couche transparente en al2o3.
JP6102810B2 (ja) 2014-03-25 2017-03-29 富士ゼロックス株式会社 帯電部材、帯電装置、プロセスカートリッジ、画像形成装置、および帯電部材の製造方法

Also Published As

Publication number Publication date
DE602005025782D1 (de) 2011-02-17
US20090087858A1 (en) 2009-04-02
EP1791982A1 (de) 2007-06-06
EP2186908A1 (de) 2010-05-19
US20060078924A1 (en) 2006-04-13
US7642055B2 (en) 2010-01-05
EP1791982B1 (de) 2011-01-05
US20100203545A1 (en) 2010-08-12
WO2006034387A1 (en) 2006-03-30
JP2008513028A (ja) 2008-05-01

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