ATE431433T1 - Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen - Google Patents

Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen

Info

Publication number
ATE431433T1
ATE431433T1 AT05760637T AT05760637T ATE431433T1 AT E431433 T1 ATE431433 T1 AT E431433T1 AT 05760637 T AT05760637 T AT 05760637T AT 05760637 T AT05760637 T AT 05760637T AT E431433 T1 ATE431433 T1 AT E431433T1
Authority
AT
Austria
Prior art keywords
present
encoding
reaction
ligation
sample
Prior art date
Application number
AT05760637T
Other languages
English (en)
Inventor
Mark R Andersen
Kenneth J Livak
Adam Broomer
Caifu Chen
Original Assignee
Applied Biosystems Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Biosystems Llc filed Critical Applied Biosystems Llc
Application granted granted Critical
Publication of ATE431433T1 publication Critical patent/ATE431433T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT05760637T 2004-03-24 2005-03-24 Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen ATE431433T1 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US55615704P 2004-03-24 2004-03-24
US55616304P 2004-03-24 2004-03-24
US55616204P 2004-03-24 2004-03-24
US55622404P 2004-03-24 2004-03-24
US63068104P 2004-11-24 2004-11-24
PCT/US2005/010184 WO2005098040A2 (en) 2004-03-24 2005-03-24 Ligation and amplification reactions for determining target molecules

Publications (1)

Publication Number Publication Date
ATE431433T1 true ATE431433T1 (de) 2009-05-15

Family

ID=35064420

Family Applications (2)

Application Number Title Priority Date Filing Date
AT05760637T ATE431433T1 (de) 2004-03-24 2005-03-24 Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
AT05732398T ATE399884T1 (de) 2004-03-24 2005-03-24 Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT05732398T ATE399884T1 (de) 2004-03-24 2005-03-24 Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden

Country Status (6)

Country Link
US (2) US7601821B2 (de)
EP (3) EP1727913B8 (de)
JP (2) JP2007530048A (de)
AT (2) ATE431433T1 (de)
DE (2) DE602005014453D1 (de)
WO (2) WO2005094532A2 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1552001A4 (de) * 2002-09-19 2006-01-04 Applera Corp Verfahren und zusammensetzungen zum nachweis von zielen
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
ATE431433T1 (de) 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
US7364855B2 (en) * 2004-04-30 2008-04-29 Applera Corporation Methods and kits for methylation detection
US7427479B2 (en) * 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060019288A1 (en) * 2004-06-30 2006-01-26 Applera Corporation Methods, reaction mixtures, and kits for ligating polynucleotides
EP1791982B1 (de) * 2004-09-21 2011-01-05 Life Technologies Corporation Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
CN101351542A (zh) * 2005-07-15 2009-01-21 阿普尔拉公司 液体加工装置和方法
US7745122B2 (en) * 2005-07-15 2010-06-29 Applied Biosystems, Llc Analyzing messenger RNA and micro RNA in the same reaction mixture
WO2007024798A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Apparatus, system, and method using immiscible-fluid-discrete-volumes
EP1924713B1 (de) 2005-08-24 2011-11-09 Life Technologies Corporation Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
US10829803B2 (en) * 2006-05-10 2020-11-10 Dxterity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8597938B2 (en) * 2007-10-12 2013-12-03 Qiagen Sciences Llc System for providing control reactions for real time RT-PCR
WO2009100449A1 (en) * 2008-02-08 2009-08-13 Fluidigm Corporation Dynamic array assay methods
EP2326732A4 (de) * 2008-08-26 2012-11-14 Fluidigm Corp Testverfahren für erhöhten durchsatz von proben und/oder targets
WO2010028288A2 (en) 2008-09-05 2010-03-11 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
JP5608998B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
CN102449169B (zh) * 2009-04-01 2016-03-16 德克斯特里蒂诊断公司 化学连接依赖性的探针扩增(clpa)
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US8574864B2 (en) * 2009-11-05 2013-11-05 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of RNA
US20120202704A1 (en) * 2009-12-07 2012-08-09 Illumina, Inc. Multi-sample indexing for multiplex genotyping
WO2011100057A2 (en) * 2010-02-09 2011-08-18 Eugene Spier Methods and compositions for universal detection of nucleic acids
NZ608313A (en) 2010-09-24 2013-12-20 Univ Leland Stanford Junior Direct capture, amplification and sequencing of target dna using immobilized primers
WO2012054933A2 (en) * 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
US20120288857A1 (en) * 2011-02-03 2012-11-15 Fluidigm Corporation Multifunctional probe-primers
US20140170653A1 (en) 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
WO2012154876A1 (en) 2011-05-09 2012-11-15 Fluidigm Corporation Probe based nucleic acid detection
EP2710145B1 (de) 2011-05-17 2015-12-09 Dxterity Diagnostics Incorporated Verfahren und zusammensetzungen zum nachweis von zielnukleinsäuren
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US20140242587A1 (en) * 2011-10-03 2014-08-28 University Of Florida Research Foundation, Inc. Rapid and Reliable Detection of Infectious Agents
CA2859048C (en) 2011-12-30 2020-08-18 Daniel T. Chiu Chromophoric polymer dots with narrow-band emission
US10648030B2 (en) 2012-01-13 2020-05-12 Affymetrix, Inc. Methods of determining the presence or absence of a plurality of target polynucleotides in a sample
EP4361607A2 (de) 2012-02-03 2024-05-01 California Institute of Technology Signalkodierung und -dekodierung in multiplexierten biochemischen assays
ES2655509T3 (es) 2012-02-14 2018-02-20 Cornell University Método para la cuantificación relativa de la secuencia, la expresión o los cambios de copias de ácidos nucleicos, usando reacciones combinadas de polimerasa, ligadura y nucleasa
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
WO2014022827A1 (en) 2012-08-03 2014-02-06 California Institute Of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
US10011862B2 (en) 2013-03-14 2018-07-03 Cornell University Method for relative quantification of changes in DNA methylation, using combined nuclease, ligation, and polymerase reactions
EP3126523B1 (de) 2014-04-01 2020-02-19 Cornell University Nachweis von dna-methylierung unter verwendung kombinierter nukleaseligationsreaktionen
CN106879252B (zh) 2014-06-10 2020-07-17 德克斯特里蒂诊断公司 用于收集和稳定生物样品的装置及方法
US10344321B2 (en) 2014-10-08 2019-07-09 Cornell University Method for identification and quantification of nucleic acid expression, splice variant, translocation, copy number, or methylation changes
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
CN108026568A (zh) * 2016-01-31 2018-05-11 阿费梅特里克斯公司 通过结合条形码标记的多核苷酸探针进行核酸分析
EP3472354A4 (de) 2016-06-17 2020-01-01 California Institute of Technology Nukleinsäurereaktionen sowie zugehörige verfahren und zusammensetzungen

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
ATE173767T1 (de) * 1992-04-03 1998-12-15 Perkin Elmer Corp Proben zusammensetzung und verfahren
WO1996015271A1 (en) 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
WO1997045559A1 (en) * 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US7014994B1 (en) * 1999-03-19 2006-03-21 Cornell Research Foundation,Inc. Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
CA2398107C (en) * 2000-01-28 2013-11-19 Althea Technologies, Inc. Methods for analysis of gene expression
EP1313880A2 (de) * 2000-05-30 2003-05-28 PE Corporation (NY) Methoden zur detektion gewünschter nukleinsäuren mittels kombinierter ligation und amplifizierung
US6620586B2 (en) 2001-02-20 2003-09-16 Applied Gene Technologies, Inc. Methods and compositions for analyzing nucleic acids
US20030119004A1 (en) * 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
EP1319718A1 (de) 2001-12-14 2003-06-18 Keygene N.V. Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen
US20060088826A1 (en) * 2001-12-28 2006-04-27 Van Eijk Michael Josephus Ther Discrimination and detection of target nucleotide sequences using mass spectrometry
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
US20040214196A1 (en) * 2002-09-19 2004-10-28 Applera Corporation Methods and compositions for detecting targets
EP1552001A4 (de) * 2002-09-19 2006-01-04 Applera Corp Verfahren und zusammensetzungen zum nachweis von zielen
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US20040110134A1 (en) * 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
WO2005026389A2 (en) 2003-08-29 2005-03-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna
ATE431433T1 (de) 2004-03-24 2009-05-15 Applied Biosystems Llc Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen

Also Published As

Publication number Publication date
EP2161346A1 (de) 2010-03-10
WO2005098040A2 (en) 2005-10-20
EP1727913B1 (de) 2009-05-13
ATE399884T1 (de) 2008-07-15
EP1727913B8 (de) 2009-08-19
DE602005007874D1 (de) 2008-08-14
US20050272071A1 (en) 2005-12-08
EP2161346B1 (de) 2013-06-19
JP2007530048A (ja) 2007-11-01
EP1730312B1 (de) 2008-07-02
JP2007530051A (ja) 2007-11-01
EP1727913A2 (de) 2006-12-06
WO2005094532A2 (en) 2005-10-13
EP1730312A2 (de) 2006-12-13
US7601821B2 (en) 2009-10-13
DE602005014453D1 (de) 2009-06-25
US7604937B2 (en) 2009-10-20
WO2005098040A8 (en) 2005-12-01
WO2005094532A3 (en) 2005-12-22
US20050260640A1 (en) 2005-11-24
WO2005098040A3 (en) 2006-03-23

Similar Documents

Publication Publication Date Title
ATE431433T1 (de) Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen
HK1098789A1 (en) Method for detecting and quantifying rare mutations/polymorphisms
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
DE60133111D1 (de) Verfahren zur erkennung und zum assay von nukleinsäuresequenzen
Islam et al. Optical biosensing strategies for DNA methylation analysis
ATE494389T1 (de) Universeller tag-assay
DE50015854D1 (de) Oligonukleotide zur schnellen Bestimmung von mikrobieller DNS/RNS
Lee et al. Single-molecule visualization of ROS-induced DNA damage in large DNA molecules
WO2007002567A3 (en) Selective isolation and concentration of nucleic acids from complex samples
ATE414096T1 (de) Verfahren zum nachweis und zur quantifizierung von analyten in komplexen mischungen
WO2005094532B1 (en) Encoding and decoding reactions for determining target polynucleotides
TW200720441A (en) New labelling strategies for the sensitive detection of analytes
KR20080084029A (ko) 앱타머를 이용한 표적 단백질 검출 방법 및 검출키트
WO2005047543A3 (en) Ligation assay
ATE546550T1 (de) Verfahren zum nachweis von hpv sowie sonden, primer und kits
WO2006128010A3 (en) Quantification of nucleic acids and proteins using oligonucleotide mass tags
DE60023539D1 (de) Immunologischer nachweis von rna:dna hybriden auf mikroarrays
ATE377091T1 (de) Verfahren zur erkennung von nukleinsäuren
WO2007115242A3 (en) Binary probes for fluorescent analysis of nucleic acids
WO2005010199A3 (en) Invasive cleavage reaction with electrochemical readout
ATE553216T1 (de) Sonden, bibliotheken und kits zur analyse von nukleinsäuregemischen und verfahren zu deren konstruktion
GB2539675A (en) Reagents, kits and methods for molecular barcoding
Wang et al. Colorimetric biosensor based on a DNAzyme primer and its application in logic gate operations for DNA screening
Cho et al. Detection of mismatched DNAs via the binding affinity of MutS using a gold nanoparticle-based competitive colorimetric method
DE59914868D1 (de) DNA-Nachweis über einen Strangreassoziationskomplex

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties