ATE431433T1 - Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen - Google Patents
Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülenInfo
- Publication number
- ATE431433T1 ATE431433T1 AT05760637T AT05760637T ATE431433T1 AT E431433 T1 ATE431433 T1 AT E431433T1 AT 05760637 T AT05760637 T AT 05760637T AT 05760637 T AT05760637 T AT 05760637T AT E431433 T1 ATE431433 T1 AT E431433T1
- Authority
- AT
- Austria
- Prior art keywords
- present
- encoding
- reaction
- ligation
- sample
- Prior art date
Links
- 238000006243 chemical reaction Methods 0.000 title abstract 5
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 239000000523 sample Substances 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US55615704P | 2004-03-24 | 2004-03-24 | |
US55616304P | 2004-03-24 | 2004-03-24 | |
US55622404P | 2004-03-24 | 2004-03-24 | |
US55616204P | 2004-03-24 | 2004-03-24 | |
US63068104P | 2004-11-24 | 2004-11-24 | |
PCT/US2005/010184 WO2005098040A2 (en) | 2004-03-24 | 2005-03-24 | Ligation and amplification reactions for determining target molecules |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE431433T1 true ATE431433T1 (de) | 2009-05-15 |
Family
ID=35064420
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT05760637T ATE431433T1 (de) | 2004-03-24 | 2005-03-24 | Ligation und amplifikationsreaktionen zur bestimmung von zielmolekülen |
AT05732398T ATE399884T1 (de) | 2004-03-24 | 2005-03-24 | Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT05732398T ATE399884T1 (de) | 2004-03-24 | 2005-03-24 | Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden |
Country Status (6)
Country | Link |
---|---|
US (2) | US7601821B2 (de) |
EP (3) | EP1730312B1 (de) |
JP (2) | JP2007530051A (de) |
AT (2) | ATE431433T1 (de) |
DE (2) | DE602005007874D1 (de) |
WO (2) | WO2005098040A2 (de) |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2006500034A (ja) * | 2002-09-19 | 2006-01-05 | アプレラ コーポレイション | 標的を検出するための方法および組成物 |
US7570443B2 (en) | 2003-09-19 | 2009-08-04 | Applied Biosystems, Llc | Optical camera alignment |
DE602005007874D1 (de) | 2004-03-24 | 2008-08-14 | Applera Corp | Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden |
US7427479B2 (en) * | 2004-04-30 | 2008-09-23 | Applera Corporation | Methods and kits for identifying target nucleotides in mixed populations |
US7364855B2 (en) * | 2004-04-30 | 2008-04-29 | Applera Corporation | Methods and kits for methylation detection |
US7575863B2 (en) | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
EP1761637A2 (de) * | 2004-06-30 | 2007-03-14 | Applera Corporation | Verfahren, reaktionsmischungen und kits zur bindung von polynukleotiden |
JP2008513028A (ja) * | 2004-09-21 | 2008-05-01 | アプレラ コーポレイション | マイクロRNA(miRNA)の二色リアルタイム/エンドポイント定量化 |
CN101351542A (zh) * | 2005-07-15 | 2009-01-21 | 阿普尔拉公司 | 液体加工装置和方法 |
DE602006018472D1 (de) | 2005-07-15 | 2011-01-05 | Life Technologies Corp | Analyse von boten-rna und mikro-rna im gleichen reaktionsansatz |
EP1928571B1 (de) * | 2005-08-22 | 2013-03-13 | Life Technologies Corporation | Vorrichtung und verfahren zur mikrofluidischen steuerung eines ersten fluids in kontakt mit einem zweiten fluid, wobei das erste fluid und das zweite fluid nicht mischbar sind |
ATE532878T1 (de) * | 2005-08-24 | 2011-11-15 | Life Technologies Corp | Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas |
EP2677039B8 (de) * | 2006-05-10 | 2022-10-05 | DxTerity Diagnostics Incorporated | Erkennung von Nukleinsäuretargets mithilfe chemisch reaktiver Oligonnucleotidsonden |
US8597938B2 (en) * | 2007-10-12 | 2013-12-03 | Qiagen Sciences Llc | System for providing control reactions for real time RT-PCR |
WO2009100449A1 (en) * | 2008-02-08 | 2009-08-13 | Fluidigm Corporation | Dynamic array assay methods |
US8697363B2 (en) * | 2008-08-26 | 2014-04-15 | Fluidigm Corporation | Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags |
US8583380B2 (en) | 2008-09-05 | 2013-11-12 | Aueon, Inc. | Methods for stratifying and annotating cancer drug treatment options |
JP5608998B2 (ja) * | 2009-03-31 | 2014-10-22 | 東洋紡株式会社 | 保存安定性に優れた核酸増幅検出試薬キット |
CA2757300C (en) * | 2009-04-01 | 2018-01-09 | Dxterity Diagnostics Incorporated | Chemical ligation dependent probe amplification (clpa) |
EA023190B9 (ru) | 2009-04-02 | 2018-03-30 | Флуидигм Корпорейшн | Способ мультипраймерной амплификации для штрихового кодирования целевых нуклеиновых кислот |
US8574864B2 (en) * | 2009-11-05 | 2013-11-05 | Epicentre Technologies Corporation | Methods and kits for 3'-end-tagging of RNA |
EP2510126B1 (de) * | 2009-12-07 | 2017-08-09 | Illumina, Inc. | Indexierung mehrerer proben für multiplex-genotypisierung |
EP2534263B1 (de) * | 2010-02-09 | 2020-08-05 | Unitaq Bio | Verfahren und zusammensetzungen zur universellen erkennung von nukleinsäuren |
AU2011305445B2 (en) | 2010-09-24 | 2017-03-16 | The Board Of Trustees Of The Leland Stanford Junior University | Direct capture, amplification and sequencing of target DNA using immobilized primers |
WO2012054933A2 (en) * | 2010-10-22 | 2012-04-26 | Fluidigm Corporation | Universal probe assay methods |
WO2012106668A2 (en) * | 2011-02-03 | 2012-08-09 | Fluidigm Corporation | Multifunctional probe-primers |
US20140170653A1 (en) | 2011-04-15 | 2014-06-19 | Life Technologies Corporation | Chemical ligation |
CN108342453A (zh) | 2011-05-09 | 2018-07-31 | 富鲁达公司 | 基于探针的核酸检测 |
EP2710145B1 (de) | 2011-05-17 | 2015-12-09 | Dxterity Diagnostics Incorporated | Verfahren und zusammensetzungen zum nachweis von zielnukleinsäuren |
CN103890245B (zh) | 2011-05-20 | 2020-11-17 | 富鲁达公司 | 核酸编码反应 |
EP2764100A4 (de) * | 2011-10-03 | 2015-09-16 | Univ Florida | Schnelle und zuverlässige detektion von infektionserregern |
CA2859048C (en) * | 2011-12-30 | 2020-08-18 | Daniel T. Chiu | Chromophoric polymer dots with narrow-band emission |
WO2013106807A1 (en) | 2012-01-13 | 2013-07-18 | Curry John D | Scalable characterization of nucleic acids by parallel sequencing |
BR112014019168B1 (pt) | 2012-02-03 | 2021-10-05 | California Institute Of Technology | Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos |
JP2015508655A (ja) | 2012-02-14 | 2015-03-23 | コーネル ユニバーシティー | 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法 |
SG11201407901PA (en) | 2012-05-21 | 2015-01-29 | Fluidigm Corp | Single-particle analysis of particle populations |
US9791372B2 (en) | 2012-08-03 | 2017-10-17 | California Institute Of Technology | Multiplexing and quantification in PCR with reduced hardware and requirements |
EP2971172B1 (de) | 2013-03-14 | 2019-07-24 | Cornell University | Verfahren zur relativen quantifizierung von veränderungen in einer dna-methylierung mit kombinierten nuklease-, ligations- und polymerasereaktionen |
US10221447B2 (en) | 2014-04-01 | 2019-03-05 | Cornell University | Detection of DNA methylation using combined nuclease ligation reactions |
US9757095B2 (en) | 2014-06-10 | 2017-09-12 | Dxterity Diagnostics Incorporated | Devices and methods for collecting and stabilizing biological samples |
JP6871160B2 (ja) | 2014-10-08 | 2021-05-12 | コーネル・ユニバーシティーCornell University | 核酸の発現、スプライス変異体、転座、コピー数、またはメチル化変化を識別及び定量化するための方法 |
US11118216B2 (en) | 2015-09-08 | 2021-09-14 | Affymetrix, Inc. | Nucleic acid analysis by joining barcoded polynucleotide probes |
WO2017044993A2 (en) * | 2015-09-08 | 2017-03-16 | Affymetrix, Inc. | Nucleic acid analysis by joining barcoded polynucleotide probes |
US11117113B2 (en) | 2015-12-16 | 2021-09-14 | Fluidigm Corporation | High-level multiplex amplification |
US10066263B2 (en) | 2016-06-17 | 2018-09-04 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4988617A (en) * | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
US5494810A (en) * | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
DK51092D0 (da) | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
JP2775346B2 (ja) * | 1992-04-03 | 1998-07-16 | アプライド バイオシステムズ,インコーポレイテッド | プローブ構成物および方法 |
WO1996015271A1 (en) | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
GB9609441D0 (en) * | 1996-05-04 | 1996-07-10 | Zeneca Ltd | Process |
EP1736554B1 (de) * | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion |
US7014994B1 (en) * | 1999-03-19 | 2006-03-21 | Cornell Research Foundation,Inc. | Coupled polymerase chain reaction-restriction-endonuclease digestion-ligase detection reaction process |
US6331393B1 (en) * | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
EP1257664A4 (de) | 2000-01-28 | 2006-04-05 | Althea Technologies Inc | Verfahren zur analyse der genexpression |
WO2001092579A2 (en) * | 2000-05-30 | 2001-12-06 | Pe Corporation (Ny) | Methods for detecting target nucleic acids using coupled ligation and amplification |
US6620586B2 (en) * | 2001-02-20 | 2003-09-16 | Applied Gene Technologies, Inc. | Methods and compositions for analyzing nucleic acids |
US20030119004A1 (en) * | 2001-12-05 | 2003-06-26 | Wenz H. Michael | Methods for quantitating nucleic acids using coupled ligation and amplification |
EP1319718A1 (de) | 2001-12-14 | 2003-06-18 | Keygene N.V. | Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen |
WO2003060163A2 (en) * | 2001-12-28 | 2003-07-24 | Keygene N.V. | Discrimination and detection of target nucleotide sequences using mass spectrometry |
US6960436B2 (en) * | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
US7176002B2 (en) * | 2002-05-16 | 2007-02-13 | Applera Corporation | Universal-tagged oligonucleotide primers and methods of use |
JP2006500033A (ja) * | 2002-09-19 | 2006-01-05 | アプレラ コーポレイション | 標的を検出するための方法および組成物 |
JP2006500034A (ja) * | 2002-09-19 | 2006-01-05 | アプレラ コーポレイション | 標的を検出するための方法および組成物 |
US20040235005A1 (en) * | 2002-10-23 | 2004-11-25 | Ernest Friedlander | Methods and composition for detecting targets |
US20040110134A1 (en) * | 2002-12-05 | 2004-06-10 | Wenz H. Michael | Methods for quantitating nucleic acids using coupled ligation and amplification |
WO2005026389A2 (en) | 2003-08-29 | 2005-03-24 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna |
DE602005007874D1 (de) | 2004-03-24 | 2008-08-14 | Applera Corp | Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden |
-
2005
- 2005-03-24 DE DE602005007874T patent/DE602005007874D1/de active Active
- 2005-03-24 US US11/090,830 patent/US7601821B2/en not_active Expired - Fee Related
- 2005-03-24 DE DE602005014453T patent/DE602005014453D1/de active Active
- 2005-03-24 WO PCT/US2005/010184 patent/WO2005098040A2/en not_active Application Discontinuation
- 2005-03-24 AT AT05760637T patent/ATE431433T1/de not_active IP Right Cessation
- 2005-03-24 JP JP2007505241A patent/JP2007530051A/ja active Pending
- 2005-03-24 WO PCT/US2005/009882 patent/WO2005094532A2/en active Search and Examination
- 2005-03-24 EP EP05732398A patent/EP1730312B1/de not_active Not-in-force
- 2005-03-24 EP EP09004414.0A patent/EP2161346B1/de not_active Not-in-force
- 2005-03-24 JP JP2007505187A patent/JP2007530048A/ja active Pending
- 2005-03-24 EP EP05760637A patent/EP1727913B8/de not_active Not-in-force
- 2005-03-24 AT AT05732398T patent/ATE399884T1/de not_active IP Right Cessation
- 2005-03-24 US US11/090,468 patent/US7604937B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2007530048A (ja) | 2007-11-01 |
ATE399884T1 (de) | 2008-07-15 |
DE602005007874D1 (de) | 2008-08-14 |
WO2005098040A3 (en) | 2006-03-23 |
DE602005014453D1 (de) | 2009-06-25 |
WO2005094532A3 (en) | 2005-12-22 |
EP1727913B1 (de) | 2009-05-13 |
US20050272071A1 (en) | 2005-12-08 |
US7604937B2 (en) | 2009-10-20 |
WO2005098040A8 (en) | 2005-12-01 |
JP2007530051A (ja) | 2007-11-01 |
US20050260640A1 (en) | 2005-11-24 |
WO2005098040A2 (en) | 2005-10-20 |
EP2161346A1 (de) | 2010-03-10 |
WO2005094532A2 (en) | 2005-10-13 |
EP1730312A2 (de) | 2006-12-13 |
EP1727913A2 (de) | 2006-12-06 |
US7601821B2 (en) | 2009-10-13 |
EP1727913B8 (de) | 2009-08-19 |
EP2161346B1 (de) | 2013-06-19 |
EP1730312B1 (de) | 2008-07-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |