HUE033361T2 - Új pirrol származékok, eljárás elõállításukra és az ezeket tartalmazó gyógyszerkészítmények - Google Patents

Új pirrol származékok, eljárás elõállításukra és az ezeket tartalmazó gyógyszerkészítmények Download PDF

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Publication number
HUE033361T2
HUE033361T2 HUE14178016A HUE14178016A HUE033361T2 HU E033361 T2 HUE033361 T2 HU E033361T2 HU E14178016 A HUE14178016 A HU E14178016A HU E14178016 A HUE14178016 A HU E14178016A HU E033361 T2 HUE033361 T2 HU E033361T2
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HU
Hungary
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add add
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added
ihe
mmol
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HUE14178016A
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English (en)
Inventor
Tiran Arnaud Le
Diguarher Thierry Le
Jerôme-Benoît Starck
Jean-Michel Henlin
Anne-Françoise Guillouzic
Nanteuil Guillaume De
Olivier Geneste
Imre Fejes
Janos Tatai
Miklos Nyerges
James Edward Paul Davidson
James Brooke Murray
I-Jen Chen
Didier Durand
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Servier Lab
Vernalis R&D Ltd
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50023639&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HUE033361(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Servier Lab, Vernalis R&D Ltd filed Critical Servier Lab
Publication of HUE033361T2 publication Critical patent/HUE033361T2/hu

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5383—1,4-Oxazines, e.g. morpholine ortho- or peri-condensed with heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/4709—Non-condensed quinolines and containing further heterocyclic rings
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/472—Non-condensed isoquinolines, e.g. papaverine
    • A61K31/4725—Non-condensed isoquinolines, e.g. papaverine containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene
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    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
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    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
    • A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
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    • A—HUMAN NECESSITIES
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/10—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing aromatic rings
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
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    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
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    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/10—Spiro-condensed systems
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    • C07D498/14—Ortho-condensed systems

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  • Psychiatry (AREA)
  • Hematology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Plural Heterocyclic Compounds (AREA)
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  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Description

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Bel«2 ínhifeUras alceaáy krtown ir» ihe literatura tiñere ase thc 2~( \ ,2,3,4-i«trah>'drotsc.ujuinoIine-2“Cai'honyl}-4-Í5uiphon}'lo!iíi>aíVioyS)pbi:?tyl eosnpounsls desoríbed. tn WO201U \623P5* thc \ ·( I ,2*Χ·$4etrahydmisoqujnüÍiac>2-eaytxmv] >-2 J/í«trihydrexy-plnntyl eomprasndn deacsihed in W’<)20t}6-:'023778 and thc e-jc-s L A3 AhetiahydsOído-· ijutimliue-2<st?hnny l)pluray i |sskís»lizmc compounds dcséábed i» WO2013¡$ 10K*tth They ars all nl'poSesUiai inifrast in thc treauuesst ni'cáncer: llt cádinoc So bdng stew, t.he compouacls of tbe prasem istt calis m have prn-apupt&amp;tie propertics making it pussibie io «se them in pathoíngses ¿m,china a delect in »spopk>itfe*·. such as* !nr exampie, in thc IrcatmesU nf cáncer, autoimmiiae citseases and dísei^pjflhe· imaiune system.
Use ¡vesem mwsU'en suatea mese vspouaih te ^smposuJs nf fraseada xiv
I whereln: ψ A; represeam a hydrogen or isalogen aiom, a imeas' m branchcd (€s-€?,}-pcl>hatoaiky i gs<u.sp( a linea» ,»r branehed KYkVkslkyl gsYasps»r o e>es<vJhyí gn>up. % Áf «paseata alinear ar branchcd {€s-€<.)alkyl group optioaaily substitrapl iby á gm«P scleetcd ünm· halagctsi.'hyiipmy, %m$ pr branched fCrbjpalfeaxy., I®·!!4 and morphollncg or Λ> represen** a linear or branched CC^-C«)poiyhaloaJkyl group or a eydopropyi groop, ii being uadarníood that R* and R'\ each índependetttly of tire olher, represen! a hydíognn. aloro or a linear or branehed íCrC-palkyl groop. ’Φ Ϊ represaros a hydrogen moni, a linear or bmnched (C¡*( <.)alkyt group opoonally sobstiimed by írom une ro time halogcn monis, a grcnp (ΓΚ',Ο^Μ'^^η ér a eroup U s'<.Ym!k>l'0Ro. * R« and R.. each mdepankntk ni the odiei. tupiesen! a bydtogen alora or a limen or hranehed (CrQ)alkyl groop, or Rj and R.' form wiih tK* imrogen moro earrvlng íbero a beíerveydoalkyl, ♦ R.i repreaents an aryl or heíeroary! group, i?, being ímdpsstood that one or íppM carbón atoros of the prpnedlng gmnps, or of rinde ppssibie snbsmuppfs, tony be deateraíed, # lii rgprosenis a phenyi groop, a. dsbyinóxypkÍByi prnup, m d-fiproamd-m dtoxyphern I group, a ü"bydroK>pynm?dísH. group or a 3-hydroxypyri.dine group, it being understood ti mi role or more carbón atoros of riso preceding groups, or of duro possibie sobstnrcnto, non be demerated, R$ represaros a hydrogeo or halogcn aiom< a linear or branche i (!>! VaRvi gn»np, or a linear or hranched (fV(\Kdf.oxy groop. t R¿ ,e n estro > * > Jsogtm nmm οι a i n ¡r o han. btd o" lk\ 1 mo n m Ra and R<< each represen! a hydrogen atoro and (Ri,,Rc) ib no togeí.ber whh the o.o'boo muros carr>np. íbero a M-diosnl.mr murro »>. a L-kdnuroue picaro, or R·,. Rc and R.j eacii represen! a hsdrogen atoro and R», representa a hydrogen or inuogeiv aloro or a medio.vy groop, ur R.i and R¿ cnth opios» ot a í \ iu\»η η η, < κ , kstnts a ! \ώι pon or haiogen muro and Rc represems a hydraxy or medios y gro up, or: R.a and R,j eaeh represen! a ipdiui.cn .nono R*. u pro-roe a ht Jorro ^ rvlU'<ro group ,md R, represenK a halogcn aturo, it being underáU'Od iLn - l'ared' meaos a phenyb naphthyl» blphenyl or imkmyl group. - 'Rielmomyr meaos any mono ί'ϊ boeu'he groop componed oí Rom 5 lo 10 nng memhetx, h&amp;viog al seas? one aromatlc moieiy and ooniaming írom 1 lo 4 helero alonas soledad i rom oxyge-m saiphor and niírogen (ineludíng qualernary nUrogens), ® kydo&amp;lkyr mesas any mono- or bbeydk, «puowTmiaíIc, catfeocydk group comaining Irom 3 ίο ΙΡιΙηρ^ρ^ρ» - '‘‘heieroeycloalky!^ meaos any mono- or bi-cydlc, oon-aromatie, eondensed or spiro exoup componed oi irom 3 to 10 rmg memnem and coniaining Iroso 1 lo 3 helero atoms soleeied írom oxygen, sulphur, SO, SO» and nlirogam íi beiog possible for ihe arel, heíemarvh cydoaíkyi and hetmjcydoalkyl groups so defumd and the groups <úkyk aildayh ilkynyl amf alkoxy 10 be substituted hy fegfttl lo 3 gkidp ^dornO tumi hnaar or hmnehed (i ¡Pddkd. íCoOarnro, linear or hmaehed O' rí'sKalkoay, iCrC&amp;}aíkyi-S-> hydroxv, oxo (or Aboxide where appropmdey nitro* eyamn kOORk OCURk NR'R!!, linear or hranehed l€r-€.\s)polyhaloalkyb inDuoromethoxy, AVlYedkylsdpbomk halogem aryk hmemard, aryloxy, aryUhio, cyeloaikyL heteroeycloalkyi optidkly snhpltnied by <«ne groups, lo íheiv enairtíomer* and diáskrmiisóriirs, and ío addiiñm Mita theridi wiik a pharmaeeuikally acceplabk udd or base.
Among the pharrnaeeutieaiiy aceeptable acida ibero may be mentioned, wilhoul hnplyiog ímy limitalion, hydrochlorie acki hydrobromie asad, suíphurie aci4 phospinmk acid. acede aeid, írillnoroacetie add. lache arad, pyruvie acki makmk aeid, suecánk aeid, gkiark add, fumaric addYadaioaniC mfleidap|||k|MospMs; ΐβοχΛει mMi methanesufphonk add, camphoric add de
Anmog ti* phannaeeüdeally aceeptable bases there may la; meutkmed. wiíhout imple mg aro Imiitauon s«\h«m hydmxule·, po* tsoum hxdromde, umtmdnuno, im-bats lasóme en. \dsanUuieoux|y, A¡ sopsesems atpdmpeo ,dom ora meSOyl group. i' ven more prefembly. A* and Ar both represen! a meihyl group. {« si prefcrréd amhodimetví «>is{he invcmiott, i «epresents a (mear or braudkd κ\ ·0<,>»ίΚχΙ group. hvanother preferred esnbeeámen?, Ϊ represonts a group (€ j •COaiky i>NR sR¿, amt aspre ggpgiytg g igrodp * í~<. %>alkysR> wherefa R- ami R2 forro xxhh {he nitrogeo earr> íng Otero a heunOcsa loalkyl fn preferid compramos oí the snvendorn T representa a ntethyl, aminornethyb imorphoH»~ o shim |j\ s4 íu! i' ptpae m > m* mi ^ .navphoht' *x!víhx‘ \2 ,μ i"i> * yi)eih«m jmethyk hydroxymethy|.. p"(dirnelhylamíoo)eihox>]meb5>L hexahydso* pyíaísslii«[2s i Jí5?íaídlM'· 1¾^ 1 jíl¡, jI* :#: t'roorphoiun4-yl )propyI or milnnmmdhyí group. kven more preRrahly, T representa a Pnorphoün'4-yi ímeíhyl, rnedyvt or 34morphoUn-4-yi)propyl grmtp. bv<m soorc nuuerabK. mal R* e.uh repujo ni a indívgen aloro and {R>Jí c iogeiher svitb the carbón alones carryíng ihene ferm a kluikixolane gmnp; or R*? R« and 1¾ eagR represen! a hydtogen atore and Re representa a iuüogen, prderahly a chlorioe or Onorinc atona
Even more oreierahhe Re repPiSestts α4οχνη^^ρΐΜ^Γ§ι·οο;ρ, lo prelerred eooreoreab. o the retesasen, Fb prefetabH represóme sí group vbk\| toan pbeoyK i//· pyrazülc, I //-«tdole, l&amp;unda?;ok\ pyridme* pxrimidloe, 1//-pyrndof2,3-b)pyrkiine, 2,3-íMbydro-1 #·>|>ymdo| 2x3-b jpyríUine> 1 /£ben&amp;nu<Ja‘/oie, \B~pym>l®i· lié* pyrmlof 2,3-e jpyrid i fte, 1 //-pyrrolnf a^^jpyridme. 5//-pyrro{o| 3χ2-ί/|ρ> rímidme,: ihlopbonry pyraz.irse, pftjtyraxoh«j 3,4»£)p> mióte, i ,2-oxtagoíe, and \ H·pyra£olo[ 1,5··-irjpymnidine, Otos* gurupa opiiouulh has útg one or inore sobstóoetos sróxied dore halogen, linear or branched (LYC*ttlkyl« linear or brattehed (CKY}aikoxy, cyano, byoippropyl, éxeiáósb íétrahydmiintag -CO-O-C-Hy, MdautenOmethyk 3Rmbrphoisn-4~ yljeihyf and 2morpholin-4-yl>ihoxy. Ntbie prefcrably,. 1¾ repásentea ipip l-meibyl-li/i'N'azsd· i >1 pyddin-4-yL ! -oseihyi- i/bpyrroiojdJb>Yysidin->yl 3-eyuno-1 methyl-Lb-px,no-3-s'\ ''resano-í ?-dí nsthxl-iíí-pxtroi->-x{, 1 -rectin Ire 3 dlhsdsu 1/f-pyr.roU> [ J'h]po«dno x o' ' ν'- ere *. recual 1-pudenteoomethx!) 1/bpx'το', b\.¡ ñofcuwl vVíui^uíuLn <Η.οοο1κ*ρ to Mis mxentxm aso moiudnl so síu? Sodomup proup* ~ 5-(>chíoro»3'i!OSV3-(OK^hoHo-4->i(neíh>nO,4'düi\drossoqHmoUn-2{t//V YÍ|carl«ínyi \ pbeny! }-A-(4-hyd?s sxyptenyl)- \ 2kdime?Jiyf-:¥-(1 1 tf-pyraaol* 4-\A- l//-pvn<4e ϊ cadvxamisk, ^xUoro 3-(|Γ'£?-' nv&amp;\! lk d»h>dtotMtqiii^lx*^^ }pheny||« Λ -(4-h\ dro\> pheny IV í ,2-tlaoeíh\ o Λ -f pvvsdin-4 -> IV l /Aporróle - * -eaiboxamkie. ~ Λ?*<4-Η)?4ΐΓθχ>·ρίΐ6ηνί)-1,2"^δώγ^ί¥~υ^ηβ%·|>Ι//-ρ)ί^χ>Ι«4^!)''5'46-||(^-.'ϊ* roiorpholoO'4-yhtK4hyl}-k4“d4yv<.'hxíi5íKp5.5SK45n--2{ 1 /A-yl|carbonyf \ - 1,3 -basxodsoxol-S-y l}-1 H~pyrwL·- 3-carbexami.de, »: f?^fcliges«^ypi)eny$)&amp;^^ 2( I //)-)1 jcarbonyi)-K3-benzodicsxo!-5'yl)-íV-(pyridm-4-yl)-l //-pyrrole-.^ carlx^xsiroido:, -- S-íd-ileose -2- > 1(3M- Hmojpholm-4-> imeihy l3~3v4-ddsydfosM>q«sfso1lfs~3f 1 /A->1jera Iπmy\HdvsnIVA-í4-hvdro\>phomu- I.2 -di sneshsI - V (1 -mcü\yl' \ 4-yi)- i.//-pyrro!o-3-a;^sxamide, y1)cavbonyi} phtanis-AH'í ”^Vdroxypheny!)-1-nt^yl·!./#-pyrroíopv3-/»]pyric)in-5->3)-1 /^pyrrole-3-carboxam iét* - 5-(5-chko^'2-^(3k)'3-ímoq?holm-4-ybnodiyi}“3,4-dí!jy#él|pQiSÍ®olio43pl^ yl }cartx*n> i} pherq u · Y~í 4 - - h> oh o.x y p hí? v%y I) ^ 1 ^ 3 y í|i ο ΐ e ü ΐ y ií (3 í >-1 pvrrolo-5 - carboxanás.'kx * 5-<;S-clüoo>-.?.- U(3 x5- -y osorpkd sn~4-y Is'xxin i>- 3,4-diHydf oAorsemoisn-2( IIñ- y!jaarfei>ny1ÍpbenylV<V-(5-cyf.mís-i~íne(hyi-i //“pyrro!-3-yl)~/yH4-hyxln>xyphsnyh- 1,2 -dimeshyi-1 H-p\s rolc-J-csdíoxamitio, - N-o-*o , so-i-scem-,' 3, fcr;oM-v‘> MkikkxoO >js "Ά'3 % *0'pk> oa4 s\ oxxhvi) k4«dibvdroi,v'q$.»to!ísi-2(5 / Ay, \|o>srhosryl|pix'm A-.V-¡4-h>ο^χγρΗοη'·Π- 1,2-diroedn-!- i iV-pym>le-3-carbox¿«!ikie, - 3-( '-chb>o>-3- {|(* xv3-< niorphoíín I -yimcth)! > 3.4 dihydsmxoquhmlm-2( i //)·> 11 carbuny 1) pheoy l)-/V-(S-eyarsm 1,2-íf iimdhy 1 - I H-pyrrol - 3-y 1P/V-s 4-hydroxy phenyl)-í *2xdlmethyl -1//- pyrro le-3 -ek^pxinddo;,: ·» 5 (5-chloiO'-2 -1 [{35Ί--3-{moiphol in ·Ί-y Jmeihyt)» yi je&amp;rbonyl} pheny! hAH*I diydfoxypbeny | v 1 ^-dimethyl-AH i -m€:thy:i-2x3*<iili>!d«> 1 //«pymdop^jpyrtdfo^yl b 1 tf-pynote-3 -carfeoxami'íUq ' Λ -i 5-cyamb 1^dimethvl-1N~ρνηοϊ··Λ~γ\)·54>ñmm-2- {«3&amp;>3dmorphoim~4-y!-meihyi)“3^-diísytIroLs<x|airío!in~2{1//)~yl)csdKi«y1^phe«yl}-iV-('-1''^ydrox>iphenyl}' :|;j¿^te%l4^pym>k‘3--cadKyxamicle, - $-(5-chioro-2-'|f(^-s,3"3-(ntoipholin“«1“>'lmeÍhy!}-3,*1”dihyíÍroísoquinoÜn-2{.í/.f)·· yi|c<uixxs>nj)ha>vh“ν~^Η·ν»Γ>'>·'3-«χ<0'}Ι-ν i ikiomcreovbnIbd //-pirro! ^ \t\ \ \ MnoU'-viphenvl· U^mxl^M/f-p^iv^iO^^aibí^aaník, thcir cnaníiomers and diasiercoisomers. amí adillfo» salís ihereof wit.h &amp; |3hal*d®dd|kiiÍ|y: accepiahíe acia or base,
The Inv^otioa íislatesyilp io a process for'fhe pfppaiation oí copApqpids oí |||:· whích proee«f· l^dNafai^agd in tiutt ihesv ;s uidd as síartmg mjeitf&amp;j} 1h# .|0^pp«d.:if:: formula CII >:
!1 vvbereio E:s, Rs,5 Rc ansí 1¾ are as ciefmsd Cor lomuda (1), whíeh compound of .formula 1.11} is subjeeted lo a Heck rcaclioo, m an aqueousor organie médium, jn íhe preseuee of a palhdmm eafulvsf oi a base·, of a phoaphme and ni fue compound of formula (III);
I! whdTttn tlm gronps A$ and A? ii i &amp;r l¿múb!a (I) and Alk mpresants a Ifetar m
Imanelmd (CmCktafltgL to eosnpomiíl í# íormnla i!V}í
(IV) wherein A;, Aj, RSs íU, R, and R,· are as deíksed ibr formula {]) and Alk ís as delira;d hereeibrdríre, tlw akk'hydcr íunctioo oí which cwnpouttd oí tomada (i V) is oxiUiacü to &amp; earboayüc ruad to form the enmpoumí oí formula (V);
üV) wbcrem Λξ? Ág, R*, Rs;. and %iM&amp;Má^WiM-4tá^máié&amp;|pí^A|i$&amp; §s deiíned hereinheiore, xslneh roospnood oí fornmU\\) k títóo swhjccicd lo peptidc coupUng voth o voropomd oí fotmub (Vi)
(V!) 'as deOntd for formula i I h tojibld itte ebÉtpoúftd oí iormuia i VII):
Pf xvhcrom As, A-, R„ R;·., R>, R,o í' and ÍV au deRaed foi ío¡muía 0? and Aik ia as da Π oed he reinbe tora, tila o\te< o \\u ' o| x'hoh \ »" *»¡sao oí κνη n la (Vis ' h> olwd n ao’a Ju. C0TT0spond:.na eanboxske aeld or carbuxyhíte, vviudi may be eooveríed o no &amp;n add deovad ve snob a.\ sho coneapoonmg aeyí ehloride os asdsydrkie be ib se beiog eoupíed witb ai' anhe \HR A wku o Rs ard R, ss<¡se sho vanv meínsng'' a^ hr ioomia íh, .o su id fhe essmpound ¡a ibnmua (1). wtrkh compound of formula (h mas be purilkd aceotdmg to a convetukfnal scpatnñoa ieehmque, whkh ís convened, if desired, ímo its atfdhmn salís with a pharraaceutieaBy aeeeplablp liekl Or i&amp;tse and whteh is optionatís aepamíed tato iís ssnsners acmráing v» a conventional separaban techníquc» tí batng tmdmiood thab ai any time considerad appropmte m the course oí the above-deserihed proccas, eertaio groups (hydroxy, ranino. .} oí i he reagenCs or iníemiediaies of sx miañas may he proteeted and *hvt* dvponu.ied aeeordtng to 'te mjatrements oí symhesís.
More particular!}', when ouamí íhe groups R¡ or R< of the anime Mlk¿&amp;4 ís snhsimned by a hydroxy Íuncíkm, the saber may be subjecied betbrehand to a proteeiion reaction prior lo-<my eoupliug with the earboxylte «ud Jbrmed l'rom the compound oí lomuda (VI!h or wUh.. a «,οΐτο\ροηϋηη> sd derixatíso kxaxok tbe r-.Mktmg proteeted eonrpoone o» ^rntsb ÜV subsequently undergoes a deproteetion reaetíou and is then opdonally conversad ínto one '>f its addiUon -mlts vvith a pbamt.teuttumU <u*eptahk add or base
The compound* of formuiae Mlb lili), ti!’}. CjV% .{VI/ and .the aniírse NRRA are eiíher eommerekdiy ovaüabb or can be nbdinei jby the pemon skílíed in .the art trstóg «Pnyentlonal Chemical reacdnns deseríbed rníhe limraitrre.
Pharmacological study oí the compoands of the mvetuion has shoxvu ihat thgy have pro-apopíotíe proponías, 'lite ahíhtx to reackvate the apoptotk procesa in canceróos celis ís oí mafor tbetttpetdk interest m. tíre íttatsnent oí cahiers, aato-lmpnme dtaéhses and diieaüil bf the immtmc sysiera.
Mosr e\peesalh, the compouuda meordm- to the ímentíon ntU be manuí m the Ueaimom of ehemo- or radio'resistant eanet-rs, and sn siudígnam haesnoputhíe* and smalbceh iang cáncer.
Mmotig'Éise^ir i\m&amp; may be ώΐββΐ0]θ^^''»1^ίιίΙήΐρ^ι§. any límitaüon, cancera of the bladder, hrairg breas! and úteros, choróme hmphoid lenhuemias, roioreetas costees. «anee^ of the «"Vphagua and oxeo hntphoHanuo íeokaemu'-. non Modgkín íyntplomas, moknomng niallptpt baemoptthks, myekunas, ovarlart cancar. non-Mnall-ceU Idng cáncer, pástale: sotall-ed! kmg cursiir.. u:80k--:
HndgLm lympNwnas. there imy be rfteiíieséá more pnei'erably fóíi^skr iymphpsab rnaolle csíÍI lympbowas, dhfiuse largo B-edi bmphomas, email lyatphoeyík' lymphomas ailMgiilw Bmcü Hwphoma.'v. l'be pksetd B^iete aíso m phm»aeeáíi^ ^mpositíons corapmtng a| feast epe eotPpouríd oí formula (1) hi eosrdthuaioo with ene or more pharmaeeuPcaUy-acceplabie; exeípbms \motu? the pharmaceuttcat eomposstknrss comed ing lo tho m.\ entrón 11¾ re mas be memioued more especia! iy ihose that are sanable ibr erálj parerderaf nasal, per- or inatv-etitanceus, rectal, pedmmmm ocalar or {espiraban. sdnmtisíraiion, eapeeialh tableta or dragéesr sublingual taKcv tuchen. caguen, capsules, glosante*, kvengex stsgpositonss, crearas, oíntmenLv dermal gels. and driokalíiMli^glilléi'^p'ÉM,. .:ÍÍi^= -5sseii3^ii.J oi the patierd, the admmbhabost órnate, she ppfprs of lite iherspeutic itulkaüott, or of aoy assoetaíed tmtdnre^^ iopt 0,01 rng lb;! g per 24 honra in ons or atore adrninkimtióM, f'uríhermore, the presen!. inveiUton reíales abo to the associadon oí' a eompound of formula (!) with un andcunccr a geni sefeeted from genotoxie agems, mitotie poisons, anti-meíaboiUm,·. píasteasome IphiMígm, ifnap fuíiMíom and antihodtes, and ikb ;íp pharmaceatka! compobdons comprblng that type oí aasoeíution and i he ir ose m the mapufaeím'e:pf pMdbmftehts fpr use ipahe tfearptirn pf
Fhe vontpoumb, oi me sawmísoa as ss ab- be osed m assoetauon sstth multo! harapo m Ote rreannent of caneen
Finally, the componnds of the iaveadon raay be Hsrked lo roonocionai aníihodks or tegmeila:SáaEÍ^>láE ptoieñss thal cao be related or aot to monoelomd mdibodkne
Atuibedy nagmems nmst be unüfcrsiood as fragmente °£ Pv, seFv, Fab, F(ab!)2, i|a|%-ssdwK’ f- pe m i'isbxo^.K whi.i genera’ \ naw fríe \o.nv speedkn'. ot bmdmp as ibe mPibody from which they are descended. Aecording to the presemí ínventío», mtübody ftagments u( iho invcntio» cea be ohtained sUuting from amihodtes. by nteshods saeh as digestión by enzymes* sneh as pepsin or papa i n, nnd/or by cleavage of the disuMde hridges Os chamen! reJocpon ht uuothu mannety the antüxxh fragmenta eompmed m tbe prese ¡a invendon can be obialned by seehniqyes of genetie reeomhimnion iikewixe web known to the person skiiied i π the mi. or dse by pepüde symhesis by mesas of, for cxmnple, automatic peptide synthesix.sm such as those supphed by the eompany Applied Biosystems, etc,
Scaiibíd proteins ihaf cap he rplated or no!. to monoelonai andhodiex are undersíood te mena a protein dan eootains or not an irnrmirmgiobuhn foki and tbat vichis a hindíng capuats similar to a mooodomd amihods fbe man sidkd m the mí knowN how to sdect the premio seaííold Mmv pan kularh, h m known that, to be sdeeted, auch a seaffold lheníá.éi^^p:'.^Mf'Áy^ is tbliow (Ikerra A,;í j. Mol. Meepgn., ÍSs iOOCi, Íd7«if!fi: phytogenetkaliy good consemtion, robus? arehkeeture svith a sveil-known ihree-dimensional molecular orgaai¿ation ísueh as, for example, crystaitography or MMR), sntáll sixe, no or nniy a lovv negree of posí-transíationa! modifieailons. easy to produce, exproxs and psn ity. Snob a protein seaífold can be, but wíthout hmitudon, a stmoture sotectod buró tbe «ronp eonsístíng mhbroaeetin rnta preferenilally the ieudviironeetbr tvpe III dontain (bNfrsiO), Ppocalm. anlieaÜn ?Skerra A,, J. Biotechtiul.. 2001, 74(4):257-701, the protein /. derivative from. the domaos B oí staphylooocoal p.rotmn A, thíoredoxm A or any protein with a repeated domain sueh as an "ankyrm repeaf* pKobl et ai., ΡΝΛΗ, 20«si, vol.lüO. No.4, 1700-1705), "armadillo repeatA "lencme-rieh repeaf or “tetratricopeptide mpeaf. Títere eouíd aiso he menhoned a seaffoki derivative from toxina (sueh as, for example. seorpion, inseet, plan? or mohoso soxm.s) or protein iohibitors of neurona! o i me oxide synthase tPÍN). i he tofo*' r,ot\.,aijons arnf Fvunpíes abombo d;e invenínm \m imut hsmímg s‘ ,n .un O ay. faplirMN» I' 4kldorrvl'-f 4'lctlmy«&amp;rhonyl|-1 uhy!-tll~pfrraKI" ;1^Í|ibes«o?« aeisl &amp;id : Ethyí /. í-dmnnhyl· ¡Ηψγ^ί&amp;~3~αί?Βϊΐ\νί(ίϊί° Το ;t «sshsihm m ííf g oí ethyl 2-sssethy)·· 1 íó-pvy¡oleUmarhoxvUte (65.3 mnu d} .sssd if>5 mL <130.6 ssssool) oí snethU íodsde írs 70 mi. oí dmseüiysíommsnsde placed as 0"(' the re are added, in three portiorss. 2.61 g (05.3 mmol} oí sodums hydride s NaHi 00 U,. '1 he 'uleh b thcn stsjsod at O'Y ios ¡ bo*! l'hcn, Ose leaeusm snsNsust se (od-oYcd o\ Ose ¡uidition sil'420 mí oí iee-eold water. The reacia so mixture is Osen dshssed whh eshU »<* mu\ sueeessiveh wsixhcd -wtth P.IM raqucou* huinchas'se <n sd ,:((1} asOsseos.. e&amp;turated aqueoua UCI sokítkstl áttd -ttak&amp;rine. The organic pitase is Osen dried over hsgSOs. fshered,. eoncentrated (o dn ?u:ss and psrrífsed hy sihronsis(ogmphy over «sisea gel .dPetroleum ether-'AeOEt gradiessll. SI1 NMK: δ (400 MHz; dsnso-dív. 300K): 0.65 (d, 1T1 pyrrole}; 6.3 (Id, 1H parróte); 4.1 (1<6211,OCH2CH.O: 3.5 (s. 31 í N-pyrruie); 2.4 (s, 3H pymtie}; 1.5 (it 3H OCHjCHj) 1K; v, Ύ (s. soK8otn\v f'-CM1172 au"! /} ¿-dimeíh) le .·. «ohsmm oí (o v.oí Ose compulsad ohsained hs Stop \ (o .'.8 ssnsH'h us o5 mi. oí V..V-dissset hylaeeUsSüSík si se re are ss.scce.ss.svdy added i 5,2 g oí .Tbromom mhkirohessxukkíssyde soO 2 ' ds, 12 ' g i pe.^nisss aeetmv, v s o sm \d¡ sd 0 tl\ i skh s\ \ssmd os\*i» argua lor 20 mmuim Hiere are thesi addtd 1.2 c oí palludmm v.duly.yt l\(( í(Pn¡,'; (3.14 mmol). Use reaetsosi mixture ss (Hess heased at. Í30°C overnigist. The mixture is isllowed 10 retisns (o assihiesit temperáis.!re and is is Osen dilatad vdth díchlnrosnethasie. Xsmoaí ohsteoal ts e,\ied s" e per g 0' pseumts asd the mdeh * Y reo as unbeni lessipene ese íes i ho«s assd thv.' Í0íí."cd Use asynesn olíase isso x^s^ds.d w al oí e oxx hlpSs) and eom s sed so osxsk^ Ihv < s.ste ^oossm * se eK H sud S'· pssrilsed bv dmsmatography sner síiica gel (pesrolessns ether AcOíl gradipiOT The ilite prisduei is olisúned iss the form oía solid. *ί§ ΝΜΕ; b (400 MÍÍX; dmso-d6; 300Κ): Μ (s, lli, formyik 7.91-7.69-7.61 (d, 311* aromado i XV. to X- Ht ρνοοίοο 4,2 (φ 3í i OVÍU;Hi í; 5,4 ijk MI €livX-p>uvie). J NN M, B 1 p\rr«>k*v I 7H (!. 31 !> Ol 'H'< ’Hjl
Stop Γ: A soludon X prepared eomaining 13.85 g oí úse eompound obudmxi -a Sien B (4 > mraai) and 35,7 mt (336 mmol)oí2-nwthy b.Vbidene in a mixmre compeled rd 20 mi., or acekmc and 70 mi oí teifuhvdíohnun Iheie «no ndded, Χορν. ¡μ>. 20o mi oí tn .npaeoo vdmit'n contamine; «» huBme m lll g ni *odnun ehkniíe tNM ;OX U4? miro*) and 14,5 g oí sodmm huí agón phoaphan. (TvdlPi>d i 1M > mnmB íhc haieh is then unos otado s! u sed a? umbient lempon-Hure lor / honra, f he rcncíion mixture is s.hen concentraied na remove dte auoione. Qhyí acólate i§ addcd. and íhe oíganse pha.se ss washed with water, dried ove? Mg5U.s and iban conceturcued lo dryaess The residuo ís thcu taino up in a ndniun.no oí cihsl oihcr íhe solíd Shan obuimed ¡á tihered o t í» washed o Uh eider and bren dried X vacuo at 40fC oveniight. The «Üe producá i&amp; obteioed in the form oí'a so!M, ΛοΙ Í8 '.uhsr^nomfx uned wu.ruun hemg oihenúue puntkd. 3l! mm; d (400 Mllz; dSiso-dé; 300Kb la dtp 11) COOMV 7.*5-7*-7.4|*d,dd, wd. 3íb aromaiic lis); 6.3 (s, 111. M pyrrole); 4.15 (<p 211, OOÍS€IX); 3,25 (s, mt CH.?-N-pvrrole); 2.5 ts. .HL Oiv-pyrroie); 1.25 (i. 31!. CX.hHClIj) M; v; -OH: * 101625(81 enís acid; v: :><>0: 16¾) cm’! ester ó aeid líSIÜlldA 2~f4XEthoxy«!%onyli4^!olliyKI7E¡jyrroK2^1Ife:ed«oteaey 1 he proccdure b; in aeeotdanee w;í leche proééss oí ÍXeparaikm i, repiaeiag, on ihe ooe hand. the etiry) 2~niethv|-! //-pyrro!e'-3~carboxyhue used in Smp a by ethy! 1 /7-pym>ie-3-carboxylaíe and» on thc olher hand, the 2d-nu.uuo~4u:hknn4>nnnnhkh>dc nnud in 5iky B h>: P-bronio-bersaldehj'de. llMiÉig#: 4C!tl6»i~|4-í®tfeoxyenrbonylEl»me$hyl« ll^yrrol^yI|fe«»x»^ ribii;
Iho procedure B in neyordaneo with íhe procesa oí Preparaíion u repino ing thc eth>! 3' ií!e!hybl/T-pyrrole- '3 -carbox>la!e usod m Slop A hy «thyl i Wp^mde^.^-carbaxyfek» dartaryile brid
The groeedure u> actóoroantócrwtíh ihe proso-ss of Psepararíon k repiadrtg, ao the <me-haiKt, ihe edvyl ITmeihvl·! late «sed «i St>,-n Λ b\ t,oh\i Ubp>u,>k" >· ios κ Miai> < o s o ' U\ v d e* Οαηο5 ihe 2:*br»nu -4 *, Mes ,!prs< nVokJí chV *r Skp Η K 6"bíorno -! ,3 bersí'odioxoie'·:' k:irjtód&amp;hyde. IR: e: -OH: 3500 -3300 om'(add: v KM>; 1688-1670 c&amp;f* efcíer f add
The po>'.. salero ia m aeesndame vsith ihe procese of Preparados 1. stspiaesngdn hiep A. on too ene luaid ihe dh) i 2-« «5íhy I - i //-pymde«3-carboxylai# by eihyt !/&amp;pyiTole»3~ eachoxyiule sanh on ihe oíher batid* ihe sneihyl sodide hy eihyí todíde (see íbe pfddg:# deseríbed in ? !b o ,2 58,800 B i v
Preparativa JO 4-Odoro~2~ [ 1 -«yelvpropy 1-4-( ethosyesrbvayIH- add lito pjvocd'JK' k m .tv.u>rdam.e Hkh ihe proceso »Hh eparation I, replae;ng m Stop ,\, on Too ene iomsl tho asml Τ-η,οόΗ 0 i/Tpymdod-i'nrbsKAÍMo b> Uh>! 1 //~pvm»!e-3-
earhoxylate and, on ihe oiher batid. tbe niethyl iodide by eydopnspylhoronic add (seo ihe protoeol doaenbed m Bepard S, «y úL jonrnai of Urganíc < hennstry 'Abiten o4 H-P444, ililT
Tbe proeedhnrix in aoeordance ivbb ihe proeess of Preparativa 1, repladng in ^:m" ihe me has d ibo *. \l ' tele HA p í «e ^ euihoxx o1 o> s i'it A'^^ok ·> eadv^ii «o and, o t dv pió r hand tbn modo) íoduf. hi ssopr yoI lod de íscc ibe pntsocoí desoubed hi i'ikada b e; ni. Iklenvien:-' OH'), IT;>.'441,19b2 s iS-dlmethyl*i l^pymt^-jrtlbeíixak
«M i Híí'proeevH * ·" s * ux»wxP kí ^<*χ hv. k<.m oí P <. χ na pp «. uy a sk ' ' 5|m til#! 2>roeihybI //-py rrok--3 curboxyiate by inethyi ~>'ϊηίΜΡνΡΙ//'ρ^ίίοΙ<ϊ"3·ο;ΐίΡοχν1®ΐε' and alsa Uk .Mpomo~4<bi.acRT¡aaidxi?>de nmú m :Step -¾ i|y "24>f0a^*4m β IR? v: Λ)Η. ΡΊΓ^'Μ c«n ' ..cal. v: 'k O: R8" cm s
Prepara tkm % b~f 1 »j2H[1*m*yÍexY)ethyIMTfctbos Y«arbo»YIpSmHhvl' t M-nyrml-2<-y 1} -1 j3" d lo x; n le- S -€ a rbnry Ik ndd
StepÁ; Eiky! (^(^η^κψΙ^χφί'ηϊ^γ^ΙΗψ^Ύ^^^α^χβα^·
The procedure h in ávméímm xvith ihe proecss ofStep Λ oí Preparaüen LjYplphig the mathx! huíale uxoP ae ¿úkx lauoy agont by bee/x \ 2'bromnethx! oüicr. %f MÜMt i--(408 MHz; dmso-dé; 30GK); 7.32. (k 2H< aypnpík .1¼ .H meta berasyí ffedl 7.3 lf:L arornaüc Η, H pttm bmsryl eíher); 7.2.3 uf» 2Μ, arbMbc IR II "#?ΛίΙβ|^1 eíhcrl. h ?3 pl i H, U- pyTroIe); r.O> id, IIP H- penóle). 4.48 {s. 2H. üiphaüc 1P\ O-ÜP-PhV. 4.15 (q, 211. aliphatíc )R O-CHrCHjK 4.1 (1, 2!L aliphaüc Ms. OMM.'HrPh); 3.7 (t. 211. aliphawe 1¼ Cllsl'HrOT.'Hv-Plu; 2,l-' nv '1!.. €Η*~ pvrtoIeV. 1.2^ u. iH. ahphatk'ík. tX'lír<W IR; x'. NC"0: lf?8d cío 1
Sm> B: fhil-Í2~(Benz}4axy}etki1^4-(t4Íwxmirbmi}4}-S~met¡t}^íH~p}>m4~2'-yi}4tJ'· .kmz04fosék»$^a*bmyfie--ú€iii
The procedarc te m accardane* wjíh thp. proeesscs oí Stops B and € ol Preparaban L repkemg lile 2-'hTomO''4~chknobeazaldehyde by i-tes0iO'T2^nngpÍins«>le»3'· cathaldehyde. TI NMU; 3 (4(H> MR/, doisa-do h'Ok) ~'on ΓΙ aroman II. !1 boa .\inx\ak ' ' *' ?rn, Uf. aromada lív U Rn/x; esht"¡. ~ Nx n>. Hí. .ínxovaiie *í H bonAxdiasolek ' H! ul. 2H> aromaiie Hs, H orího ben*yl eiher); 12.55 {bro&amp;d s„ lli, COOIij; 6.75 {§. !H> B~ py trole}; 6.15 (hroad s, 2H< abph&amp;íic Hs. 0”Ot>Og 43b (s. 2H. alijrhaíte Ib;. O-CB^-PIik 4.15 (<p 211 aliphatie lis, O-CHrCH·,); 3.7 (m, 2H. alsphaUc Hy C1J?-01rO-Cn,-Ph)'. 3.40 (p 2B.. aiiphano Hs.. OlrOO-O-OHrPh); 2.50 is, 3H. Olr pyrrolefc 1.25 3H. alip&amp;bk l is. ΙΕ: v: -OI!: 3200-3300 as:! add; v: >C3~0: 16B? cía'* add
MiiMMimJJx MM34 Íte«*ylnxy)pmpyl]^"í£íhoxyearb«»y!)“$“msdhyl-i/#“pymd“ 2-yI) •'d-flnnrtíbenKok asid I be procedure k m m.cordípiee with the: praeess oí PfepatptM« 9f replaOng tío benzyf 2-brotooílbyl eíher «sed ín Síep Λ by bern&amp;y! 3-bmmoprüpyl sther and also (he b-broinb-lj” bennxiksxok-S-eatbaidehyds·· ussá in. Stop B by-2r>brom<’»-4'41«orobeu'¿aUkhyde. m vi "OH: 32000305 cío λ id;>: >0K3: 1600 cm 5 Md
Pm?a ratina 11; ít~|4"(l20íOsycíirl.HOwÍ)'d0"bb'ocHpy1~ÍíI"pyrrol-'2~yl|'-l,34Hi».?.o-· dloxüiíí-5~««rbexyik* aeid
The proccdurc Ls Oí accordance whh fhe process oí íbeparation 1. repl&amp;eiog *be 2-bro«K>4- cilombs4aalde!iydo «sed ib Stop B by 6-br»ínoH.?3-I^MMb.xdfe45 -c;« haldehyde, I* re na raí km 12: 4»M rtbexyO“|44n«lb«xyrarbenyB-· i ,5-dbnstbyl- tííypyrrOO-ylb hen&amp;oíe aeki
The pmeeduta ls ?&amp; aecordance wkh ihe proeess «i Prépdrsbon !, rspkebig ihe eíhyi 2-meítiyk 1 //-pykededkcarboxy late u&amp;ed in 5tqs A by ntethyl .2~meÍhyjO,//~pym}Íe-3-carhoxylate and- abo the 2>bromo*4'Chk>robeaMl.í.kbyde: «mi in- Síep B by S-bromoO-oíeüíoxybens.akkhyde. ΙΚ.λο “OH: 3000-2300 cirO aeki; v: >(>:0: 1603 « 1670 cníbaeid-r esler
The prwedyre ík jn aeeordaneb wltli:l® ppeexs of Ptepunttíon i, replaeing m Siep A Ihe-eüiy! tdbéibyh! H-pyn0\t~3~<0im$\éi$ by metbyl 2 - methy l>I il· pyrrüfc-3 -carboxy fot* asid aliso tlie 2-btomO'4-chlofoben*aláehyds asad m Stop IJ by ?4iromo-2, J-dihydro-ϊ >4 ·». benzodsox so· bcas'baídehyde. IR: v; >011: 3100-2500m\s acid; v: >0-0: Ks9U a I67<1 cm"!aeid * ester
Frcoaralba 14; 4-l*l»or»-5-math«^2-|4'4aieih«xycarb«»yi)4íS-di»ietbyMW- pymstdhyí\hmmk a cid
3 0 p Ov\t,K Ήϊ* XV XX i* h tV p < >,_ v ^ i*u {víais ai I vpl - nsp ή ^yp \ ?V 2 p>uolc4 earbowlae bx meibsl 2-meihyl-1 //«pyrroie*3<arboxy]ji$e and aiso the 2~bronHj-4-ch!orobeíi?iddehydc uaed m Slqi B bv 2^omo-4-t]uos-p-Jh ί-iashrsOv! a i* v ndr
Pmiaratlen Id: 4~lilíia^S-¡iyikoxy-2-H-(melli^sycarb«»ylp;lsSdilíJj|íliyiÍI:^|^rpb I-ytfhcMok aOd Tías procedure L· ín aecordanct? xvith tho prooess of kropimiuoíi I, ?&amp;plaei«g m bicp A Ose mbx\ Xíu'ihyí-l/bpxs*ok-“-e<*rbs>vd<xíe h> methvt 2-methvbl^^ynoi^2t<ubmyíu&amp; asid also du? d-bromo-d-chloníheajraWehyde «sed m iíep É by hydroxy-ben&amp;ddehyde,
Preparado» id: 6-|4-<hthoxycsrf sasryI)-i-mefhyhS-triflnorometlty 14tf-pyrrel«2byil~ 1 >3-h c s i»od laxo k-5~ea rboxy lie acid
The proceduro O m accordance w-Uh the proeexs of Prepararon 1. rcplMlpg :m it|p A lie ethvl 2-methy!-1//-py?}oic-3-cstrbsix-yiatc. by cthyl ,2-(iní]noroiiicíhyl>-i/Apym'dc-3-s asbi.í\ibito and abo nv Noino-4-chloroben/aldohyde o sed in Step B by Odirosoo- L3-bcTiaod laxóle-5-carbaídchyde.
The procedure Irin accorüanee with ihe prooc&amp;s of Prepa radon LK reptaelng tire mcthy! iodkle by ethyf iodíde (see Ote protoco! descrlbed in US 6,258,805 Rt). b''|4~{Uthoxy«íirhos^l|?l-«8|hp!»I<frtiiiiorbiÍÉt:hyl}4/^|íyrs^U2-fl|^
Siep A; £thyi J^methyi^-Ítr^Mür^iik^pyrmk^eméúxyhm
Etbyl (29 pii Í.2Í9 nanol) (40 ?! solution in water) :|S0 mk Orlll mnt«í| pold tú i(U€ua #dte predpSpe: ferrm 1 .2-Dihronuxahy! apeíaíe t preparad aecordOtg lo MMemÍMy Ib, 91ÍÍ-ffiS; 20Í1Í té added dropwíse. Ί' he reactor is Orea .waled and heated at 70®C f§t Φ$. 'ΡΗ«»Ι^:^ιβ.^ΡΙί^ ..mixture Ls cooled, Oren extracto! wth eihyl acótate» driod ovar sodium sulphate (Na^SO*) taró evaporafed lo Uryoeas. The crud| ireaebon prndtad bbtóped m puriiid by ebrpraatography ovar silrea. gol asing baplspé gud rétkyiiredípe as elwOá, liarexpePíei cot.api.sund b obudned in Ore torra of crysiais, SH NtVlM (400 MH¡?,, dmso-dó) δ ppíre flí'il (ds ! !i); 6.52 (O, 1 H), 4,21 2 H), 3,8 (8,3 i{}, 1.2?(i,3 H) 1 *F HMíi i"400 MÍOp dmso-dO} 4 ppwn-53.9 IR tATli) cm " 3Í45 * 3.128 v -CH, Ι7Π v H O. 1183 0 1117 4- 1078 v -CF3 ¿ikw $$* >- f 1 ^x&amp;Í0-5~^«^m^ík s(íki
Iho pnwdure t\ m ouoídáKO u-uh ihe pnuneol OracntreJ m Stcp\ R anU i oí IN'áparatioa L replMíng Ote 2¿br6ñío-4-ab!óroódaÉakkhyáP «sed ® Stpp 1F by O-Preípo* U3 - be ireodiOK.ole-5-carbaídebyde. Í*fiTOratkm 1%; 6-} 1 s.yetIsyesrbí>?^yí IsyI' IH~$$rmb2~y\}~ ! ,3-líenxiKli»x«Í8-5-e^rb«xylie »ci*l
Amm' U Eihyi hf:ΐΦ^(^Εηγ^Μ’ϋ~2'Μ^Μψι^^^ΦκχβΜ^ f'dhy! .Vtm>3yyf-L//-fynnl^^^carlx>xylals? <10 g, 65.3 mmol) is dissolved i» 100 mi oí dimethx ItbmmrnkUí onder aseon tsooied m *0 C. umt ihen 3-Kooveiho\ymelh> ibta v vw C8.1 o, |.Ό« nnol) is uldcU ató ai oao«&amp;n.íhc neaoüon rrnxune o- placel onde* Mimoy. 1 here i.s íhm anded ihereio„ ai 0°<\ m ikree porüons. Nal! (1.72 g. ?L83 rumo!) ov« a penod oí 15 mmme.v I he reanima oí alare ss suma! lor ÍS «limites al i¥X\ and tlsen for 15 hotos al ambient iemperature. !i ss then poureü iok> aa ice baíh ami then exsraeted '} limos wíih eihyl aeeUUe. The «rganic pisase is waslsed 3 times voto saíurated aqut-ous liilmsns ehksride sohuion, dncb over MgSsTn díte red and then evaporated io dnnoss. '1 be 0 fiKv !ií*¡H' ^named o o adu i by ehmmntogmphy ovar miseá peí usíap peiroleum eluanís. 1 he pxpected eompound is ohtsaned in thc tona ol a« olí Mí NMR {4p0 Míte,. dmsO'dd) si pptsi; ; 33 St. 3 ilh 7 3 (h 1 Hí 7.23 \d, 3 H1 o T? (d. 1 H), 6. '5 (d, i Πκ 4 45 -v. 2 Ul 4.15 Misad * 3 ΠΤ TI n\ 2 II), 3.7 it, 2 Hh 2 45 (s. 3 II). 1.25 (OH> IR<ATR)c!#; !6h9v-C>0
Sm* B: k\íhyi i-{2~heft?y&amp;>xve&amp;}i}-$-{6~fvrmri~L*Eht>#zmfnmiE$~yÍE¿~m¿th}i-p\r?íik~ 3~curhoxyíaié ího p'ocedu-e is in ss'.tonkme v>T Me procesa oí SUp H oí Pie paral loa L txplm mg Ihe ddsmnioM-ehUsí obesaaidehuto by o-homo.IT -hessaaisoxok 5-catha lüehxde. II NMÍ1 (4§# MBi, dmass-dd) &amp; ppm 9.5 (a. i í l>. 7.3 1 .í|)jT2o iPVd 1¾ /.05 f«T 2 Mí ks Hh. na .s. Í11U- 3 ths 3 lis. 4 25 2 U\A2 (miad , 3 H), 4 u5 po. 2 Mi. 2 4 <m, 2 H>. 2.55 ss; 3 H), i .25 (í, 3 i i)
Etm Ck é-¡Py2~-EmyyimyeÍkyi} 4-eEmMymirñimyES~meShyEÍ0-py^E~2-'yÍE o'oí.YOtV' 5-e'drbifxyik acM
The procedas e in ín aeeordanoe with thc ptooess of huyo C oí Prepamiioo i > NIVIR (400 ΜΗζ. dmso-db) δ ppnu 1155 chs. \ Π), 7.)5 i-i, ) Η), 7.3 (m, 3 Η), 7.2 {my 3 Μ), 6:75 (s, 1 il)s 0,15 (8, 2 Μ), 4.1:(8. 2 11), 4,15 (quid, 2 Μ)* 3.1 (bq 11¾ 1.4 (:q 2 1¾ 3.5 (s. 3 II). 1.25(1, 5 Π· Μ (.1:11¾) mild32iK)b2300 ν 4¾¾ 163? (6 shs^iiter) vddO * eoqjogafeif
ON'kT
Pyepqrqfea 21b 6-|4~|Ethoxy<;aHíeí»yl)“Í»eihyl“$'*m'eíhy¡»líl*|i:yrroS»2-yl|«13'*l>ert.P»^ dsoMied-e ar f) o x y Id adit
The. procedan is 1) acumlaace with iht pbbbsbl descilbed IrtPpparMto 11f replMSgli)# meihyl hxikie hy elh>i ¡<xbde
Hl NMK (400 MI Lí. Imso-dOy 5 ppm: 12 49 (te, 1 Hi 7 33 0% i lis, 6.49 (s, 1 H)} 6.1? ís, 2 1¾ 6.13 fs, I 11), 4,11 :(£|xMs 2 11),(3 J9 (quad, 2 ϊí), 1.S1 (s, 1II), 1:.24 (0 1111 lili (t. 3 i!) 7Ji 6-[44^ίΙίο*>ίί,8?ίιοι»>!Ι)«Ι«'(2'*ΙΙ»ο^«ίΙι^Ι)''5»*»«ίΙί^1»Ι//-|^ΓΓβϊ-2»5!Ι}-i ,3"henmúi» xa k*S-ea rfeoxyIte a sid I he procedan.* d m ;u.eardaiie.o vdh ibe pmeess qf P.r6!>a3'atbq U* Mplagirig Ihg melhyl ioílidq %· í-fefqqTO-i-flpqrqetbaqq. *II OiMR (400 MH/„ dmso-U6, 300K} 6 ppnv 1.2.53 (bs, i H}„ 7.34 (&amp;. i II), 6.9 (s, i II), 6.16 (¾. I II), 6.10 (S, 2 i Ib 4.4 (di. 2 \i), 4,15 (quad, 2 H). 4.01 Un, 2 H), 2.Si (S, 3 H), 1.24 (0 2: II) ’V NMR (400 Mil/, dmso-db, 300 Kl 6 ppm; -322 IR; v: -OH: 1700-2400 cía ! acid; v: ' C:::0: 1689 on'1 acal: v; -(16 1213 em': py rrol-i-yi) -1,1 -be smod kvx e fe~S~ea r !m> % y lie add 6'feft .i; SJ^DMktfxypmpmmc avifi 1¾ &amp; silabeó el 2S; g af elilsjí) 3J-áÍ8lhDK:fpr«|íioaaíe (OI mippl). I» 79 mL oi' meihípffd OiCío ae added ** i sx of xpsooua *5 * < seo'uso ss^esoxkle sobaco pA 1 sfsmoll i he rene don mistare A sMred ai ambient tomp^rature íbr 3 hosas. The renomm ssíAtsax is Osen eosseesstrased b> remove (he mefhanot. A bar dissmivmg día stndissnived material bs addissg water, oqucous 5H HC! soladon is addéddb ohdbo ® pl of 5. X)lhiÍd£de^iiÉe la adáed a«d ihen fhe orgarsie phase ts wasised with klge, Afier drylpg pvgr idgill* má ceoeendanso fe dsxocvs, use fulo probaos sa ^burnsed m thc form oígl oh whssds Ib gp?| so the fpxt Step wifhisul he|«g D^mvise purUied; ?ll NÜI (400 MBx, dm.so-d6> δ pprsx 12.2 <s, I H). 4 A (i, ! B), 3.58/3.4? $%4 tf|:';2,5 (4 2 H), U'KMf.bbj)
Aleo I?; íiihyi S^S^iíí^íhaxy-^-thx^pettiítmfiíle
To a eokstkm oí- 16 mi. oí AcihoxyBmxosmdonse aeisl (135 mmoll in 40 mi. oí pt||il|'Oroñifád fdpfp: are adéexf smder argop, IfeÉl di po#tedd: ¡91X4 mñvá}> "Ihbí brixdsre oltisned 'h th«n deaied ai I1FC' ib* f kpir&amp;: Aíler ¿piuralpg to ambietk iemperamre, this mixture Is bansilmsi hy fig of Ote eobiposmd obraksed in Sfep A (61,2 snmol) la 64 sal, offeirahydrodgad Os wlieO^tbere has prevtousiy Koss adik.il hs jxaisoo'i, ti g oi eardesn llissasda/ek feo rosoeh Πιο soaotton mixture ss ssirred Ibr 3 davs ssi ambiesk iemperaUsre. Altor esmbdhtmtsosx thi reskkse Is mked ssp su a mixture of edsy! acólate áse! aqueons sodimn fsvdrogen sol písate (NaHSCXj) soksikm. The. mixture h sfrred vigóreoslo nnñl no more gas is eyqlved. Altar separalkm of the pifases, the organie plaao la teaahed soceesslveiv yritlt yaslpp safurafed 'gqppppx NaHC'(4 sdiutiou and fsnaih bnne. Alte? dnsng orer Mobil and eosxesftmdfsg t¡> ilosaeO the mié psssduct A sibilmed !ó fhe Pmu of ast sai! which is osed- id tlp mxi Stdp ddtddnt feeing otilbrMise purilled. lH NMR.(400 MHz, Umao-d6) 8 ppos: 4.84 is. i H), 4.08 (q, 2 H), .159 is. 2 Ü1156/3.46 :(2:if|J Ιϊ| 2 8 Id, 2 H). US (p 1 i||; 1.09((,6 ¡h
Ste» C: Fthyi
To a sokuion of ti Bgof (he oorfe:^«ii.itesiá. miSIep B (5§J ireuol) i ti l&amp;mL mmmm thcre aie added, émpmm- M CLCh 6,6 mí oí' aqueous 4$:::% meíhytastiip colusión f ?#* 3 omol), l'he ivjfction mhtum B stirred and geníH nehealed to umbient tempeonnre over 5 honra Aíter remmmg lo O'W §Á mi of uqoeouv 40 % motín lamine volea hm (102 mmol) and thon i 0.6 mi. of aqnooua *>0 V» ehloroaeemldehyde volution U2? mmot) ase coeh adoee d¡o5 uiv m a o opeum <, o lev iban ld‘3 i Me ooeoor πομοκ o ' oseo ai ambiem h-m permute i'or 16 homv and tltcn diíufed wiíh oihyl eco late. The organse phase is warhed witVs brlne, dried o ver MgSfL and conceutrased to dryness. The crudo nrudoc.i íhereby relamed iv purified by diromutogrtqihy ovcr video gol usiug peírolemn ether and ethyl aeenue as okutmv. The odie product i.s ohtained in i tu- torro of an osl. ‘11 AMK * lod Vil* osiH> do* 6 rpm; o. 3 I Hh 62Π id. 1 fl\ 4-55 (i 1 H1 4,15 uu 2 11}- 3-6 un- 2 H}- 3.6 m, 3 1U, 3-3 (?«s 2 1¾ 3-15 {á, 2 Η), i -25 (i, 3 Hh LOS itt 6 H)
Sfep 3; iJkri Í«me&amp;yf~:~ü~mo?pkúUmHh^)py?míe~3^mbmy$me To a soiufion of 3.S e of the compotrod ohtained in Stop € (14,05 mino!} }fL2B· idí. di íetr thjdromiao ihere are added mi of anusouv: H 5« sulphimc acid s o latida, ibd reaodon mixture iv \uoed at mnluent temperatura tur 3 honra and then dthucb vvitb a mixture of eihyl acetóte and water. <\líer xeparaüon* tíre organtc pirase is wavhed with hrinc- dried o ver SleNtu arel conconltaíed to dryncxs. To a volation of iba. recidue Ihereby ohtaine-.l in A) mi of diehlmocthane moto aje mUled a sotmhm of 13-5 mi. of niorphoiiuc (14.5 rumo!) in a mixture componed of 30 mi ofdtchloroeíhane and 3.6 mL «Í4N aqueoos rMSt-^Míoa:·^ dfcxane (14,5 mutbife^rpeit· 2-4 g of fpdium írlnyafex) borohydrlde tNaBBiOAeld <3 Al t mmol). The reaetion máxime iv vtkfed ai nmbkmt mrnperatore for 5 boom and then dibned with a mixture of diehloromeihnne and satnmied aoneoue l\xdRX>'- rol mam .Λ tíer repamiam- of Ihe phavr and extrneboo of (be mpK'oua phave vuth dk’hloromeíhane. íhe orgatúe phases are dried over VlgSCL and coucenlrmed lo drynesv i'he crn.de prodnet ihcrrhs ohimned is pinified hy ebromamn-apiu roer míh..ü ye i nving dioMpromeihane and medumol ay elndniy. The tule prodnet ív ivolated in the form v*f an oil- *H NMR (40D ΜΗ/. dmsodOí ¿ppm: 6.65 (d? I H), 63.1 (6. : H), 4.H (<6 2 H}:. 3.S8 ts. 3 H'k 3.57 (m. 4 11). 3.05 (m, 2 H), 2.4S-238(«u ó H), 1.24 ít. 3 i i) :kettzmÍim:i^e~S'Carh<¡xyik add
Bhe piocedure *x tn occordanee uith Stop·* B ,md 6' m kepamistm 1. reida^mg dfe$v«.~ '^tuo^ehlorabmziddehyüc uaed in Stop B h\ 64xomo-1.3dxo¿iHÍa¡Xi.>k'-> Mrbnídehyde, ^4RiíieearhopydH^ pyrrsíl-ls-y! f~ I ^S'-bfe.nxodi^xok^S-i'srhtí.'syiio acu! Síi'g 3: hjhyi 4t4~dL>\^&amp;X}''3'ii\'t>"fammmie
To a solísima oí 40 g oíeihji riMhoxyucetate (22? mmoí) in 67 mfie$f$fS$ se^OimtheM ÉI6ÍÉI&amp;áÉifeXiiigérlón porUoas. 6 g o( sodiusn (261 mano!). Therreaetkm mixture ts Oten; ¿msred ai .unbkm temporasme to> 48 hours. 1 hes >: *$ie noded 10 mí., oi'.mothano!. and ibón the sutxUue u.mdm!.>:sed-*tt{* 68 mt oí water, The pH of the reactirm mixtura ís adjusted tu pll 6 hy addmg IM tupeau^ MCI sulutton. 'Vhe mixture h> Rtparated atui then extractad wnh odní acetato Ote óigame pha.ws are eombsned, waslxd vdih baño, dríed ove»· MgSO*, iíUercd and eoneuur.ííod tu di> nesx i he utle pu>du<d o- »mtumed sn the tom' <0 <nx oít whíeh is used irs the next Siep vdihom being ¡uhenvBe puridad,
*11 NMR (400 ΜΗ/, ΠΧΊ0 6 ppuv. 3,o («, 2 Itk ! 28 (t. « \l). 3.7-3.6 (2m. 4 11). 4.7 (x. J H). 4.2 (quad, 2 H>
Sim B; Ethyi 2~{2.2-dietk#xymefhyf)'í~tnetHyí--pyrr&amp;fc*-3~ewbi>\yiitte k , ^ «.onpoietd msotud * ao~or >uv ouh ue phm xi.ONe.0vv o Veo t. ü Preparados^ 22, asurdug trom tho edi>] 4,4'dseshoxy- kYM-buOuKxne obumod ia she piveeding Step 5ItNMR(40<UÜk. 4mae-Jfo8pem u/n (d. 1 11).6 32 uU m.6 23 k s 1h,4P<q, 2 H). 3./ p, 5 I t)s 3.68/3.4 3 (2m, 4 11). 1.26 0. 3 Jh. 113 ít. 0 H)
The tifie compotmd k oht&amp;íned ht aecordmiee witfi (he procesa clesedbeíl is Stop D o!' Preparaban 22, starñng írom íhe eihyf 2-{2»2:-dk;lhoxymetb'yl}~ f.H^hyl^yrífcle~3-carboRylate obtaíned in (he preccding Siep. *H NMK (400 MH.?, dmso-dfc} a ppm: 6.74 (ch '! Η), ή 32 (4. S H), 42 5 Og 2 Hg 25 {>:, 2 Η), 3;65 íx, 3 H), 3J (m. 4 H), 2.32 (agi H)} 1,22 (g 3 TI) 33?/.» />; ñ~i4-(Eth(>xyeiírhtfnyÍ)"I~mefh$Í¡~$~{mmpM&amp;Míi^^íMg$h$í}*IN^ifrrú7-'2~fi!¡~iJ~ faMZiidkmtf'e-S-viwfxfxyííc sacid
The prueedure is in accordance with thc protocoi. deserihed in Steps B and € of fepMrdkm I, replncing (he 2-hromo-4 ·chlomhenzaM^hyde «sed in Stop B. % (V%n:me> í,3-lxmsodioxole-5-carhai<tehyde. ¥rm»r»ñén. 24: si)-13-heÉ»dhsxi>k'5-carbesy!k' ndd ^ÜELál Methyi 2-0?- meih^xyeih}dbd~meÚiyÍ--pynúk'-i-'Ctirbi}xyÍme
The hile u>nqx>and 'is ohtained ia aeeordaaeé: wíih the prótaeni of Sfeg O oí
Iheparáii en 2:2S esing methyi 5 -methoRy-S oxavatemle. !if NME (400 MHg drnso-dd} δ ppm: 6.69 {wd>. 1. H), 6.31 (wd, 1 IT)> 3.60 (g 3 !%. 3,50 (g 3 13),3.49 (g 2 1¾ 3.2 ig 3 10,3,11 (i.. 3 11): diifXíiÍe-S-aitboxyik’· ocié
The procedwre is in aeeardanee with (he prolocol describid in Steps B and C oC Prepararon g replacmg ihe 2-bromo-4-eld»3robtay/aidchyde uscd m Oiep B tey 6-hromO' 1 J-henRodioxoie-S-eaiihaíd^lsíyde;
EllllSIállmlSi 2-ί·Μ Ethftxyearbeay IH ,$-*Íi«wíhy I-1 .TBpyrmb l-y l| ÍRw.ok aeki The procedurc ís in acoordanee wirh thc procos oí P?epanition 1. rt piaonni dw ddaonnw-eldombeozaklehyde ased in Sí«p B by 2-bromobeox.ahiehyde, 5íl NMR (400 MIL·., dmsu-dó) ñ ppm: 12.81 (m. I Η), 7.84 ix\\ 1 Hh 7.56 ira, 1 H). 7.51 (m, 1; 1-1), 7.35 (ta, ti m. 6,25 (p; i HK-3J6 (q, 2 H>, 2,24 ($0 3 Hj, 2,5 (a, 3 M)s 1.25 0. 3 TI) íbxnnarafion 26: 5-FI««r«-2'|4-ÍKH,íboxycarho»yI)«13'd^?eíbyt»ifí'py¥'í'^'-2*‘yl]'· bíríuok a.ríd 1 le.1 procederá ss in accordance '-vnh fin,· pnsccss oí Preparados- L repiacmg in Síep Λ i he otkyl tfeyl- I //-pyrroIe-3 -omhoxjdste by meihyl 2-meíhvM /7-pyrro!e-B-x:arhoxyime and aku -he 24>rxnno-4<.Uorolxav¿aídehyde usad in Stop B hy 2-bromo-5-fhti»n»-beneahiekydo *B NMB pao M He, dmso-dá) Sppm: 7,6 (dd, 1 IB, 7.42 i ni, 2 I!h 6.22 (s, 1 B-. 4.15 (% 2 H), 5.21 (s, 3 H), 2.5 (s, 3 H), 1.23 0. 3 H) WP NIVIR (400 MI he, dmso>dó) 6 ppnn -113
BreparfBkffl 27; 0ndbnxyb«n:«ni€ add
The pmccduro is in aeeardancc with fhe procesa oi Preparation .1, rcpiadng in Siep A the ethsl e-anefhyki /7-pyrroÍe-3-en rhoxy late hy mcihyl 'd-rneihyl-1//·pynrole-3·car bcxylaur and aleo fhe 2“h?onH>'4áhlorf4>ernoddehyde used in Siep B by 2-bromO"4~meth(ixy-5' lf4>rplMaig!dó!iyd6, *H NMR (400 MHy droso-db) 6 ppro: 6.65 (d. 1 Ηκ 7.67 (d, 1 Ηκ 7.24 (d. i IB, 6 48 ;s, I 11), 4. i 9 (% 2 B)s 3 J7 (S, 3 1:!);13,35 (s, 3 ííí 2.56 (a, 3 HT 1.26 u, 3 TI),
Prepararon2&amp; 244·€ΙΜ«; ^iairlM>síy hvl Bllf-pyaTOBilyyll^'tlííOs^S» asid
The proeedurc ls- k: aceosBaneq wlih Preparatiun T, rfplpcipg ?kp d-hrosnoM'-cldiypbenyald:ehydó psed In Mqp B %v4^ilpp^>^S-nunUoxyb^^kfgby4i;. •*Ü IMIt (#00 MHl, (feiso-#) δ ppm: O.f (nit 1 H)„ 7.9 (41 H), 7,27 (9, 7 1% 9-23 p. I 1), 4.2(7«!, 2 Mk 3.95 íc, 3 11), 313 (¾. 3 Hk 2.5(sJHl ! 15 (t. 31!)
If; SChferp-iM
»«ñS í'hc proeedurc »s m aceordanco wiih -he procos;·; o* Preparador 1 repiad ng tbo ilbromo-d-ddofobenzaldehydc used in Siep B hy 2-hrosηο-5-dd mohenzabfehyde. |9'cpprp||ou ;|lh *«•4» «Idnrnibcsmde add
The pínceéum-is so soeordapee wih liso pmddss o;f Frepamtíoñ 19. r^íaplog ífeo 6*%fCtm0-· 1,3-bcuzí»dioxolo-5-c3rbakiehydo by 2#>re?no-4-dik»robe?ízaldehyde. «cid I'hc proecdure is in aecordanco with Iho process oí Prcp&amp;raíiou I, repUomg in Stop A. the oihy) 2-me?hy!«1 ¿/-pYm)ic*3«eaihoxy{aie by nwthvi 2-mcthyi-1 //^ym>k-.Mearboxyhu; and aiso ihe 2-hronso-4-cbknsshonsnddehyüc useü in Stcp B by dlnonsO'Museihoxy·· beuipldehyde:. *B mm (400 Mil. dmso-db.s 5 ppm: 12.8 iba, i Bk 7.34 mi I H\ '7.26 (d, 1 Hl 7AS #1!,! MI 6. i P (s, I 11), 314 (sil H), 1.09 p, 3 Η), 311 p, 3 112.5 (s. 3 H)
Preparativa 33; 4«jM2(|4>*fl»«rv«ihyll^pym>M-yl|-1,3 ~b a n t od im ole- S~c.a r b os y I i c sdd
Vhc procedute 1 us aoeo; dance Mi du: psouxxd < 1 Prepaíation Rh repiucing thc 3·· baennedn>.x} nsúOsyibeoeone used in Stop Λ hy .’Mxvnus·· 1,;-dUluoro-Mbané. ^methviheHzmemiphmmíe I v a solotioss oí 30.2 g n| {{.bsY L2J,^-{etFahyds\dsoqu*imlisv.l-yljnse!Í«mol i 184 nnsnd) sn 750 í«L oí díehlos'omoílpne there are succekssvdy added 01,7! g of fosgl neyotlbe (481 mmol) and Osen, dropwise, 122 3 tnf, of fofoAMrieOwfomme (740 mmol). The reactton mixture h then stnred at amblen! teusperaiure for 20 hunos. h la then dilnted with dichioromeihaoo, washed ssjccesslveiy with SM ΪΚΊ solution, satura!ed agneous Nal ICO3 aoknlon and then hs'me unid neutral. !he urgente pitase is Usen dríed mor MgbO.;. füiered tuíd eonneuíFated id Is then dlssoísíed ín a rninisnum wíutne of dlélílorfmeídand and Osen eyelohgygne Is; added s.mí|: a precipítate is formad. This preeipifote is íbess ílhered ufTastd vusshcd svüh oselnhoKano AOer dtying. thelitio prodssu N obtnined tn lite fossnofctwat.s. sil NMRí o {400 MH?; dmso-db; 300K): 7.75 ts.i 211, aForpgtk Hs» orillo O-tosyl); 7.0 (di 2H. tmamatic lis. ortho .v-tosvig 7.5 ífo 2IL aromatie lis, me ¡a O-tosyl); 7,.3 (d, 2H, aromatic Hs, meto íY-msylK 7.15 -0,0 (m} 4H, aromado H% .414» 4.15 $1% 'M%- aliphahc ! Ls. teíndiydndsoqohmlitte}; 4.25 (m, 1H, aüphatk H. UtrahsJndsí.xptatdlmeK 4sfo s s Md, 2Π, aliph me lis, ΓΙΙ-ΐΜοίρΠ {2dd. 311. sd phatíe IN sePois\dse> sm n\<'nek3 tN m 0L OSCfoFO t Ufo 2 fo ts 1H VSO. Ph- 01.2 lllt v 'SO'' 1IfoM 105 cus *
Step II; LhR^J-Meihyl^-fi^meikylphmyíkmíplítmy^JJJ^-íetmíiv^rmsoquimfim··:·.
Td a suspensión of 8. 15 g {214.8 trasto!s ol hthfom fosan untan hydride foJAd!;} in 800 mi. of rnedtyí ño'í-r-utyi ether íMFBÍo Osera are added 101.2 g of Ose ditesyi etannoossd obtainesi in Step A 0214 8 mscoil díssolved in .700 trsL of ΜΓΒΕ. The batch ss then heated as 5GeC for 2 hours, Ϊ1 se aUou-ed to crol tsssd placed at (BCfo ansí títere are ibón added, dropvvfoe, í ‘ sol oí fofo \,n h. vdiíkon, Πιο tv.fos ls sheed at jtnhvaí teooKsuhne tes --N mi sanes. ílse sood Usenos oHfoned ls sisen Hite red ->o and vvasbed wíth MI 8b' and then u jh m, hlromro lañe I ro tnt ae ro hen rotíteone tt, J te dt\nt,*s s (κ uro grodm t'- fot n obi&amp;roed m the .forro of a eolia. !I1 NV!R; a (40u M!i‘¿‘. dmscroK 50*0: '-' 'v0 <d. A uana nutro lis. roO \no seis OS (d, 3Π uromane Hs, roeij \ to-nh, 13 uO ;m, 4H< αη-routro !K termhukoisiKjiUroohml 44 tro, 3U, akpkaúc Hs, tclrahyároíscxguinoline);; 43 (m, !H, aHphattc H, !ctruh\drossoqumo!rot;V, 23503! (2dd. 21 i, aHphattc 1 !n teirahydrmtroqmnohnel 2 35 tro 314, ¿¥-.S%4*h· €11.»); 0.90 (d, 3! I, IE ; v: fofo)?: 13323154 cu#
Sim €’i üñhJ-Meiíni-iJJJ-mntávdrfmmjtítimtlme l a a sohítH.m s.n'i I. í 5 g (103.15 mm«!¡ oí fhe nronoioroA eompound dnaroed ro Step B hs 500 mí, of tmhydrous snetharso! Otero aro added, i a portrons, 3.92 g (10! snrod) oí sitagstesltros turmngs. llfo OifoO is sOrfod m fe pfoédhee of uitrasuund íor 90 Ifoum 1¾ fokttron mf^forexk'-thmvlílfoí^d.'^ #g .fálití is washed several; tíme» wi-fh meíbanol lite íthraíc is Ütest eorteeroroted to dn ñero, Alio; pssriUoaírots by dtsT.roisfogrsphy over süica gd using diehioromethtm* and arorooautdn'Cihanol ai; ehmms, the tille produci is obutitied in the forro of a o os!. 5e mam: w tm- «% 4*™^* iir m? mmm: ié, tetrífoydroisoquOrolínel; 5.90 tro. 21 i alíphatk Hs, t e lab) dr< >koq u m< s i me κ 185 (ni. 11!, aliphatse H, :tmah}drrosroq.urooImcV 5.68-2 4 (200, 2Ü, altphaíie Hs, telnihydro-isoqubtolhro): 1.12 tki, 311, teUahydfoísoquinoUne^CIIa); 2.9-2.3 (ni, bread, !H, ÜN (te í rehy droisoq s i ? a d is te) s f»i v: -NH: 3248 o» *
Skvlb' (Mti-J'Mcthyl· 1,2, a, 4~tetnikydrm$oqitmoiifíe kydmehiútMe i\> a so kit ron oí 14.5 g 197,20 auroro m the eoropoond obtamed m Stop í ’ in 30 mi oí anh\drou;> othanol Otero aro addeO. dropwise^; 100’mh of a IM srilOdon ef 140 ín efoén: tte kátóí $á. álfeÉdkaf ;&amp;r I hour and Oten fíhered. ! he erysfoís thereby obUtinerl are tvashed xvlth ethy! ether. A ker dr>'!ng. the tule prodttct ró obtained nt ihe fonn oí'erystals. 511 N*MR; Λ (400 .Mitro 2m;rod6; 100)0: 0.5; p. bread. 211, ΝΗ3* (te«rahydrí>-Hoquínotmee '022 ím. 4R monudíe lis. teU'.írodvoixngbmdhk'); 4,22 (sr 2R aliphaík: i R nv ,h$diw-Mquin*diñe\ ' O *ro ΠΙ abpkdv R tcuabyUreisoquifiolineV, ' 03Ό.4:; i2dd„ 2R ülíphaiie Rs. teroahydro¿soqronolroo; 1.20 (0, IR terrahYdrossoqehrohneRliid IR: v. -NH·/: 30Q0-23G0 em‘l ·. v: aroroalie 011' 766 ero'5 carhamste
SfefíA: Bsn^v? ($$hS'th}^n>*ymeth?th¿*4~tíihydw~lH~húqtiÍHafíne~2-cerb&amp;Ayhiie \ n vOj' roiroe »n d\PY„d tea» a i a κ 4 tro o dk íunouu R l· Rea !u,> ¡ *
Sro¡ro ii ,¡a v *» ηαί ot ni" n R !\ 2000' \tarmg ítem R a ro RO RR.4 |00§ mrnol) )n Olp iprepnspipf <md triethsWuira. ro utáw m J»v'hb*reoK'ih*me lííer eaníroairon fey ehrotnatography í>uw \{Uv.<; í.v. n\rog *\ueksrro Res rod Hh\í acetan .e. da euro Ove Me produet ss-nbtained ro the fomt of an oii. 5ΪΙ NMK: 6 (100 M1R. RMMUIro HK»K1; 7.2 Hay 5R aromatie Rs O-0«í/>R 7.1$ (n 4H. aromade Ms. tt kis'dpdrokoqromdroe); kt3 rs- 3Π. <sllrH0; 4 0 p|. IR H *etrahydro5.soqi»radinet; 4.47 (ro, R OljOls. 4 16 (n¡, iII, ti tdrahvdtomHpHnolimd; 4 22 (d, III l( íeirahRroisoquroolíneK 3.30 (dd, IR CMjORq 1.23 (dd, IR , CÍf>OÜK 23)3 (!,ki t1 f, I i tdrahydroboquinoline); 2.16 (dd. IR H letrahydrakoqumoüne) lii; y: OH; 3410 ero"5; v; <00 !<20 ero"5'vrot'oroaüe >€·Ή: 754ero 5
SrlS' (3$)~J-{awfomi>ihyi}-3,4-fiíhjr'tl?úh'&amp;qm#oHne-2{ní)-<tirbúxyiefá
l'hi.s cantpotmd la nbt.tinul romy a proroed Ira ai the Otenme e ·; O Oagé t>¡ ít¡ j R\/ í ‘Rw. 44. R8\ 20015 ronling (raro 33 g ol'dk eoropmtnd ORned i o Susp A ΓΟ.3 romol) tP Oté pt£se«ce oí dlphedflphosphotd aa;lde and tríphenyiptiosphme 30 gdluli©a !i ilil. AiWr puntiearoro bv ehromat.uumPhy over allica. gel u$£ti$ éíbep -mü etlwO .arelare :k eheaUs. the tdlé p?oihu.í ss tibtaíned in Ose rom; oían oi!. {ϋ ''Λ 11¾ b O'T' ^01,\ !íNÍSO dA V0hT ' ;f> iss· OI, «novar !|\ O-bt ltí v|), / 1¾¾¾ 411, man $ He 1A U o ¡xah > d t o i em uta te 1 h te t; 54 o v< Ά. < Hj- ho 4.x m m, n íetralmtroisoquutojineV· 4.5.3 (m< iI'Js H t e irah > d rossopu m r> h ne): 4,30 om 111, H ktr.dpΟηηρί.ημηηοίηνΑ. 3.38 ím, 310, CH>N 0 3,Pk {dd. 111, 11 kkíahyaUotssuiutnolbmp 2.78 (dd,. llí, II kdmhy d ro í soq · s i no h fiel
IR: v: N;¿: '2095 cm'4 v. <€-0:1694 em v: aromarle >€-H: 754 errO $ie&amp; €V Bewyí {3S}-j^(ámhmmeíh^i}'Jt4-dihydmiM^i^m0^fi^mrfym0kÍÍ&amp; I o a Nultmuu 1 20 o b i n at v<! o! ihv ando vO1 sp «miobiamed in Sien B in 650 mi, ol T.HF ths. :i are NUdoov>ne(\ yddul 7^5 g (^"2 mtnoB oí irspbem tphesphiut.* .n«{ ΟΆ0 of n,tte¡ Vhe baíeh O radiocd lor 2 hunt \ '0 ««ñutes, lite reaettusi mixture í.s Oten v onceo?: ated to ds^mc and Bu ',ealdue mí ts «un nAm np ¡« ¡snpropO cOter A vdnm precipítale appeitrs; it 1« Osle red oíí and washed uddi iaopropyl edter, "Ote i i i trate Ls Iben concentmed tu dtvness and iheh pnriiúi: by éitOTnatugntpísy over sillo» yel using diehloroniethane atul mat.hand as d temía. "('be dde prndiset ís ¡thtained itt Ose ibrnt oían olí llIMMEt δ (400 MIA; DMSO-dd; 3C.H31Q: 7.40 <m, 514, arontalle Has O-hen:ey0; 7.20 <*«, 411, aromaüe Hs, H tetrahydrolsoquírioUneK 5.15 (s, 211 ClIrPhí; 4.754J; |lb %%M tetmhydíxn'íoquintthiíe'u 4.'40 (d. !U, U íemihsdrtdampmolmeK 3X0 im, 311, , CflNOT; 2,45 :m, 21L II tet rahv<,lr« b soqu s no i me t; 1.40 (nt, 211, NI l·?
IR: v: NH¿: 3400X300 em'l v: <0-0: iÓgOcnO $m M: Benzyi
Tu a suba!ion oí 18,4 g 162.1 nimnl) oi'the eotnpoitmi nbíamed ín Slep C in 630 mt oí' diehlorenK'íhnne íhere tac suceessix'ely added 17.5 mi., i 124 ntmol) ni'triethyíamine and, ín porOona, 14,9 g toO mmol) oí dnkxkhuiyi dioarhoualo. l ito bak:h ís sOrred m ambienl kmperuture for 2 It. llm reaaiion nasinre is Oten esmcemmted and ethyi aeeiate is Oten stdded. l'he tugante pitase is suceesshoh washetl wuh i 01 ÍH'.'i soluOen, brine, sakuased aqueous Nal KT)« solallmt and Oten britte. AOer drviog, eormeafraUng to Jryness atnl prifyim? by dhromatography o ver tilica gol mi&amp;g pettoleutu ether and ethyi aiv-iatf: .p ekum.v the litio product i i obbnood i o dio íorm oían od *H NMRi δ sddO MHz: DMSOobo 300K); ?.35 (no 511 aroreauc ÍK Odinnrvh; ·' id i;m, 1K, A( rn.it·. i s, d tcdat'xdii"'OvnooUncL o N', :no 5 i. Mido.' Id x U1 CU' dho -t Ό ie 111 1 ιοί o svbo vcijU' ¡ moo* 4 M (no 11 , ι leen'uO >, h\ oodnO C;>y; UU Η .„*íish\(U>n\(n)uoMlmíU. 3 ^ un. Rd lí ΐνΐηΟι\ιοοΐΜ·κ|''.'η do.· ' <. llds'l UOO. 2 Ί (d. 1 Μ. H temihydmisoc|uinoiine): 1,34 (§, OH, NlIBoe) IRí v; Ni!: 3..151 coi d v: <(>0: ¡6S6c.rn¡ Síe&amp; ili terf~8uf}4 f(3$)~l¿J&amp;teimk)i&amp;#íí¡&amp;qmi&amp;lm~3yimeíhylfmr&amp;a&amp;MÍÍé·· lo a soiudon o! 21 g (53 mmob ol dio compnnnd ohtaíned in Step D m 600 m.L. oí ethyi «ivvtme tUi.lv aic .nkleo 2 1 g o? paUadh«tvonKatriho« .10 Ido Nnch »n miucO ai .nulnoni temperatura under a presare oí 1,3 bars oí dihydnogen fbr 5 hoüts, Tfe ífaqitf&amp;tlIttMíi1® Ideo fute red and then e anee atañed lo dni;:.ee. lia tifie pnxiovt i.^ obodned tn dio fono oía so! id. 50 NMRí o (400 MMe DMSO-dbi 300K); di5 ím» 4H, aronume lis, H tetrahydro-ísoquinolíne); o. 35 u i!L NlIBoe); 1.90 (no 21i Η t e ii ¿di y d nb soq u i no i i n e}; 3.00 (m, 2R OljMIBoe); 2,80 (m, IH, H letrabyUmisoquinoime); 2.65 tdd, 1H, H tetrahydíO-iaoquhmüm- κ 2.49 (dd. !Hh H tetra!iv ámbsoquttudmb); 1.40 (s. ΟίΙ/Νί ΙΙίοο) IRi v \1I Ή5ο.'304 un t M i .nmde¡, \, ' t O lo88 erfS' vi NM: i! §2#; «m 1 (ΚΠ asíinel
Preparatkm 3d{3^''3'-(4-MorphoIinyMaUi^ÍCÍ Sím.........A: Bentyt (3S)~3-t4-m0rpk»t$nykertonyt)-3,4~itihytir0-2iÍJHÍ)~w>quÍm>ÍÍne- <:ú?ha\\ f!sh> Tó o sobr. *n ol ί s οι i (hiAlto b atNmUj-1.2, M tthaindni 3 ixoqnmolineeanHsxyiie aeid id> ramal? tn 160 mC of diehlii'Oíninliase there afe added ! ."· mL oí murpholinc {17.6 romo!), íhen 9 mh mf RRARriethRdMine(64 maní). 3,3 g oí '!i51 -¿il|y^:Uíúv(EDC) (1922 rimad) and 2.6 g oí !"uifo\*.Kívv'M'¿ vio v*obb' . íc 3 cono!) The ¡eaeunr íí'kuiív Ín Ojciod ai andbeni tempetaturv overnighi. ii B thvn ponred i ato amminium ehíoride sohüíon and eximeted wiih eth.yi. -acetan The mgame phast ts then ¡ined wvf MpM )*, and thoo tihered and· evapotateci to drynexs. The ¡mide ptroduci iherehy obtained h then ptirííkd by ehmmaMgraphy over Alba ge! issmg diehionmndhane and meihanol M «Inania. The product b obiamed io ihe fono oí a Ibam»
Ti NMR; o (400 MHr; dmao-dé; 35310; 7.30 {m-, SH benzyi); 7/15 (n\ 4B< aromad;: lis);
$<2-53) Cm> 3li¿ 214 beiuyl, 11! ddtydroixoop.nnoHnt:K 4.75-4 A (2rL 3H dihydmisoquim»jmeK 3 55-3.3 (bg 811 morpboHM); 3f!2b2/i: ;(2dd? 2t:l díhydrmsoqumoiimjí IR: v >(M3: 1604;1650 cm! $g&amp;M* Bett®i{M)^-(4-tMri&amp;4tfmyfyMtkyÍfá4^ydr0-2(l&amp;)^Nti&amp;MlÍntf«rin>cytm Ί o &amp; solado» of 5T g of ¡he product ubigined -in Step A (13.*) mrnel) i» 278 mi.· of telrahydroiuran there are added N mi. of boramí-dimethyisolpbide; crmipkx (27.8 mmol) at amhiení temperamre. 1 he hateh la heaied Ib? 4 honra ai 80"€. I; ts atlowed io retare to ambicnt iemperatnre and ibero are then added 7 mi, (14 mmol) oí El bMe?S, The reactiou mixture L· agairs beated ai 80*0 íor 2 Ippp- Tlie teífghydroínrun 1$ iban evapórate*! olí and iban ibera la siowly added methanof and then 5.6 m.L oí 5H aqueous HCI soba ion i 27.8 ¡timo!). íhe mixture ¡s si irred at ambsenl tempennure ovenbght, and thm ai 8(0(7 tbr 1 honr, Samrated aqueous NaHCO* aóluUon ís then ilMWTifiteie»®· mixture placed ai 0°C mitil a olí of 8 ix sbiasned, and extrae don wrtbediyl acétate is then :^Ι3ί^.'ό«ί.·:ΐ%6:organlcr pitase ts then dried over Mg.sO*, and then ftHered and evaporated to dnnoxw fhe ütio product ts obhñned ín the Corro oíanoH. *H NMR: 5 (400 Orneo do; 353K1: 7.41-7.30 ¡uurexohed peak HI HeiwU): TN (m„ 41b aromarle Ih.i, 5 nn. 31b 211 Ivn.’Tl; 4.70 '+.20 ni, 311 dihydr-msomnm'hne); 4-58 (,m, ¡M dthydruisogmnolme); 3.50 (m, 4H laorpluníno); 3.02-2.80 (dd, 31i οιΟ'Ό-'ΗΛορίίΐΐίΌη^ y 3 42-2 P (nnresobod peak. 'bf 411 mmphoboe, Ή unapsadiurt, 3.15 (dd. III morphohsu.·) IR: v: 2810 cnf5; v: "C:-Í.í. 1694 era'5; v; >CMK>.; 1 1 14 cmk v. >CH-A.r: 751: 69? criíf*
Stetf C: 0S)~3~i4~M0rpki)ÍmjH<meikylhIf2):3,4''Mrahydmlmqídn^h'm¡
To a .sohnion oí4.9 g oí fíu.< οοτοροοιχ! ol'Síep B (13.4 mmoi) in 6? mL oíeth&amp;uul (heve is added 03)80 g oí’ pafíadkn.o dilmhmide (20 % hy xveight) al ambient temperaiure. The fhacóoii mixture is placed «ndhf L2 hara of hy díogéa.:M ÜÉ^I hóum:
It is then passed thmugh a Wharmim lllícr and the puiiadíum ís Hitas Bnaed severa! times; wsth eth mol I he i'dnaio is > \apa;a:ed te de nea* l no ok ?"o>H el <>·η<}κ od o* tLr Uasit el an oil. *11 AMR; < tkl Míe?. dn>o oe DOIO » í ^ ! 0 (m ev %ed eeah n i, asiv> »t\ 1k κ 3.92 2B teUahvdroisoquuioh.ne); 3,60 (t, 4B nierpholineK 2 98 (m, IH teir&amp;hulrei'í.aponcfüne)' 2 08 akh IB ícnaliveicisoquireline', > 2 o utm esehed peah, 8N, IH teírahydroisoquifioiíoi.c 6H morphoiine, IH MH) iR;v. 'ΜΚ3Β^οοΓ v H'-O-t'* ΠΙ^ορΓ' v Π!-\ί 74? em 5 4k (3ά^'^3~|{4~Metl¾yi”i"pli>era¾ínyi|»lethylI"Ií2,3í4-fatr¾byxtra- fep|spil|ip Πιο proeedme ís in aoeordanee whh tire proeoss of Freparaíion 32 repiaeing the morphoüne uxed m Síep A by N«ethyl-pipersxine!.
Fm-mraOon SB (3$Η$^P-fMnrph«Íiii'4-yl)ethylf-1,2Js4-fetraliyclroiantpilimite lylfieltipfllp
Stop ,1; íert-ftutpl (3S)-3-(2‘m0fpkotin<>-2-0x&amp;*tkyth3,44Íihy¿ro-lli-is0quittvlfae~2~
í'íi/'Aoat/íoV
To a aPluiloo of 1; ,f (; í) 30 mmol) of |( 35)-2 i(erfbmoxyearbony i)·· 1,2,3 A-ie:odiyd?oiSiHionMÍoeie;jaeeoe -oeid m 100 oil el dk-hkoímíeUasa. meo* ore added dropwiae 1.10 mi..· (11 32 samo!) of me-pholino, still dropesise 4.3 ntí. (30.3 narseB ot tííahsWnxíi ' '0 ¿ O" 01 rumo') o*' ΠΒ <n'd 1 V 9 0 68 "imek o' UOBi (mdsev^bonoeis: i ek) i he haích ss Bís'ed al <on 4u3 lenjpeíatyío f>vt 1Λ heue ihc οηχήοη mixture ui thus - xuth dschleunneihaoe, auccx^n-eb xxa.sked \vnh tX| IU. 1 .solutíoo, saturated aqnesstsa NaHCQ.r solutlon and ¡han briste imtil neutral. The orgauic pao'» í\ Ibón osvd o\ct MíAO; íüseruo nM v. - roernr stud su d*xmss Alto, '\oíxv4,ue hx chromaUxgrnphv mor allica gel a al na dsehlorosnethane and methanol na eíuanB, the tille pmxksot ie ohtalned m the íorm ¡' un mi *11 vN.MR: 6 (400 MIL% dímando; 300k.r A 20-7.10 (m, 411, aromad*. !L\, tetrahydroisoquínoline); 4,70 (m, 111. aliphatíe I la, CH tetrahvdroisoquinoimeK 4.75-4.20 tira, 21i ahphaóe lía. di. aípha tu K o.trah y d s ¡ usoq o mo h ¡ le >; 5.bu |m. 81L ah pitad*, IK morphoiíne): 3.00 and 2,70 (2cki 2!I, aliphatie H, letrahydroisoquinoline); 2.50-2,20 (2<i 211. aHphaik I Lv C\ K\»; 1.40 íá, o| I. *RU>
ItevrCK): 16«?; 1625 cm1
Mm Μ: I ^Í^hoim^ylb2^3Shh2Jt^etmhyJmh&amp;quimiíitf-3--yl^thuHtíne i o a 'snhuiun os 3 86 g .1 15 n; xa-»!} oí the componnd ohndned tn 84ep A in ib mi oí dsddofometlnme tdere are ndded dtunxvLse So snT 1.80 mntoh oí 1M solation oí H( 1 m ether, The baieh is uirred ¿u simbieni semjtmture íor 15 hours, and ihenthe suspenAms ls ídtered a mi tho psoupkate vtashed xuth eider. \í\er dsxsug, the tille prodoei es ohtamed m the íorm oí a solio. sIt NMRt o MOi? Mili·. dmaO'dO, 3ü0K.): 9.80-9.50 (oí, 211, NIT'h 7.5(07.fu (m, 411 aromarle Hs, tetrahydroisoquinolmeg 4.30 (m, 211. alsphaOo Hs, CTB ai pin* tu N ieiruhydtoisoqtdoohne}·. 3.80 «m, I i I, nhphutse ll.v CH tetra hx ,0 - * ¡ soma i noli ne K 5 "u-ldO siso, 6;i ehphat-e lis, >morphohneh * M and 28 (m, 41 h ahphsck II, t il.·; tetrahydroisoqninuline and C1BCO) IR: v; -NBA: 28004»0»Τί: v: <>-0: 1630»'*
Step (j f3$h3~¡2~fMorphtiÍÍn /< 2JJ~tem?hvdr#ÍwqmmÍim hydmehimife Λ sokitioo oí 2 5 g (7 44 ηηοοΠ oí the eontponnd oblaínod m Step i> su 22 mi. oí ΜI Bu ard ;· ntl -o díehkeoiiuthaoe ,n pmpared. AHot eooOag :n ,tn seo ixtth at 0oí.\ dono ate adJkd fhesvk!, 0:op\\íM . i " τη) 18 \ tsxsoB ot sM 1 nAllh -totudoss in tettahxdsafm'aa i 0, ixsích i'i íhen siirrt-d al amblen! lemptraíuro for 6 hoars. )t is placad ;u 0°C„ and ihm- is iban andad dropeenas 1 ed >a -M \'mRÍ vduíton. Vhe haioo is sthred ai amblen! n\ ue ice 1N x "sjUs Ί x. ! k \ κη ! aed t»>! »aiU* \ W1 íl 4 s o wiíh dichloi'ouictbanc and i he Obrare k> cnncemrated u> dryness. The o!t i he re h y ohumu'd i\ diluix.il o un dld do-m'.imkav md thoio me aoded, drope-ce, 0 3 nd of a. \M soiumn al Í-1C) ia eíhcr. The hatch Is süned al ambiettt tempcralur# íbr 1 hour aacl th«n Hííered. The cryeiafs ''huelo cerned alie 3 onb oda I etheí Λ he? Jeme 'he utle posmu v\ obluims.! m ha· formo!'a mjid. !II NMH. o «^0 Mil , c umOp. Tojo i '5 - 9.8Γ , 'm O* Vil·'! 10 {<0 ,m. II NIC); 1.20 (m, 41·!» aromasic lis, teira1iy\lroistu|aí«ollaoT i.30 m, 2H, oliphale 11%: C3í%i ulplm io N ietiahcdK>iv¡'x|K>a'>lak°\ a,0u i ^ s> ho c >' i aUfhain 1c, CR; sipha to 1 niocphoOne), 3 70 orn Ili ahphabe Rs, OI icmdnmoccqumolmes „\.55*3J0 (n>. 4I.L diphaoe lis, Olí a Orna m O morphtdno and i Ik-nwiphaline 1 3,15 (dd, Ili ahphatic 11 CH-t teinihydroiuoiptmohnt'): 3.10 (m, 211 aliphaik 11, CU alpba ío O morpholme); 23)0 ídd. III. .dlphatm ti OH; i ciad i> ib msoqui m d I nel 2.0> * 2,13 (2 su, 211, uÜphade 11, 011' t e u n 11 y <j ro i socf u i no 0 ne) IR; X-; NlT / 1ΙΓ: betsveeo 3500 and 2250 eiu't- v; C:::C; vyeak 1593 un’1, v; aroma lie C-i i 705 cm*
$rep»mium 00: <3^}k?-|3»fMur|>hiill»'4'yi)|'mipyllls23í4-íeíMlsy#3>k«^ilunll«e The procesa i? the sanie as ibas oí hlep Λ oí thepasanon V ,Vfem Bi mi'-BuM2-(tf3ilh2-Í(4-ttiethtfph0mrlhíttii*haH¥il· f JJJ^tahrármimíérnim- 3mt}mÉMρί}^§!Mr0iMin^4φφ^mψrψmmme: |f:t suspensión of 1 g oí NaH (60 %) (25.08 mmol) m 30 rol, oí MTBE Ihere are addesi, diopwise, a soludon of 5 g of íerí-hiny] 3onorplu>llnO'3'OSoprí>pauoat.e (21.81 mmol) m 20 mL of anhydrous CH BE This suapcmslon soned ai amhieol temperature for 1 hour and iban ihe x. cuspo me! ohíainttl ín 5u-p \ is adslen in ihe Oms oí ,s ¡env-der l'he baieh o-· s^rred .« no Γ Tos '0 0οη*\. 10o od of vimusmd ajamo·- onmsooium ehkaide Ssdmionare added. The resuídhg MlüÉon la leíase 1¾ drled over sVlgdlV tsltered and eooeentrateá i$- drpess, Afier fnimkain.m by ehroipatograplsv over sitien ge! nsiag díchlorom^hifp.-ámí MeOH as ekumsj th« expecned". produet ss obtained m ihe fono of mi od. }I1 NMR^OO ΜΙΙλ >fo$st,-do}dppnv 7 (m 7 2 ÜT 7.3- ' 2o (2d. 3 iH ” * 3 (tn, 2 i IX 7.00 t» 9? i2u 2 ÜX 4M ¿'''» i, \ti 4 28 ^AR. 3 íh. 4 7 VA Λ * Ow, l H), 5Js m*. 1 K> ' X 3 H fm *' Ih. ^'í'Xü 4 H\ 2 X (a - 7 Π- ' '3 3 «hV Mi» n$ i '*> »:n\ ' ΠΧ I W I >8t.V X!) 111 (Al Si) cm' v. a' o. P^o-m-'-.v C <> XvU (amsdei <· S02 1134-Πχ* x >€~0-€ -; IU :X y ; Η'Ή-Ar: 81^-7-16-^0¾
Sfw €? 2~(¿í3M}~2~i{4~Metk¥Ípht?m'$ftiíiphtmyif~f>2t3t4-teímhydmÍ'i{Hitimeíin~3~ vfjM<?fh¥Í)~3~fm&amp;rjf*halm~4~yi}~S~(*M'priiptwt¡L acá/ so a aoludon of'0,5 g t1 7 X? tumo i) oí Üie eompound -obíained di Stop B isi 40 mt of dioxane therr ,uc added. d'Ofmtse. 20 mi of a 4M sotunoo ofiUl io dsoxaoe. The dandi is ?sfeed :&amp;r 48 honra ioid bien íhd sohstion ss esineóiírateb 4o vis \ s.n.--s Λ fies: droiij. tlur expeesed predio.! h ohamed so dio form oían olí Ί Ι NMR (400 MI tj dmso-d6} δ ppsn: 12.75 (π>. 1 H), 7-0 {2XX 2 H},. 7.3 (2X1 2 H), ~ I 0.05 (3Xo. 4 IB- 4 7-4.: (d. 2 Ih.4.25 4.13 i2%o ! íí). .10-5.3 un.9 III 2.--3 (,J. 2 IB. 2.3 (2*s,3H>, 1.8 (1 2 H) IR (ATR) era4; v: -OH: 3500 to 2000' v; eOO: 172/ tacid); v: >C~<X 1634 SasmdeT v: -802: 13300:155
Step Di 3~f{M}~2~f(4~Mirth?íphmyÍ)suípfam}¿¡-tJJ>4^etMh}'dr(m-nqmfieÍin-^yl}-i" fmorphúíin-4-yí}prúpm~í-ett<?
To a soliuion of 7 JO g (I6.SI ai seo 0 oí Ose eompound ohiaims! in Stop C in 100 mi of DMSO diefv aro mlk\! i.lP g 101H3 mmoh oi'.sohd sodium chkirldo sNal'B smd ihc«, irppwise. 5 mL «fxvater. íXe batch is stlri'od as 4S0'XC for í hoor and Ihesx fc asakaloo k concesa rated to 11 The reaedoss nlxtore la íhon di tosed wlth dsehloromethane and washed χ' til μ«' >and aqueoui Htbnan ehlondc sotoon and, thm wsrh bnm. Ihe • a gamo phy-'c te (lien diícd oxcr MgSOi, fihtrrcd and sseneentratcd to dnnesv Álter jxa-itlcíulon hy clnosnaiography over si lien s»d using eyelohexasie and eríryi acósate as eiuams, (he eapecied proüuet is obUnned in ihe forro oían olí. *M NIMR (400 ΜΪ lz, dmso-dfti Ü ppm; 7.65 (d. 3 Hs. 73 uk 2 lis. 7,15/7 Γ2 nn 4 i l\ 4.6 íd. I 10 '1,5? id, 1 m. 4.2 (m. 1 Ih. 5 5 tm, 4 i h. 5.4 (2 m. 4 Ih, 2.o s2 dd, 2 llK 7.55 ss, 5 m. 2.3{rn»2H)t i .5 (quad.„ 2 .11) IR fAl'R) cm"!: v; KM); 1554; v: -S02: 1331-1 156; y : >CH~Ar: !1.>6?5 iigdd ¿> te$mhyé?úki}qumú7mfd 3 e '(nlnoon ot * »* 4 t.4(t πην!' oí tHo *omf>*»ra ofeiamed stv Step D ín 60íwL pi' MTÜF and 14 ral oí dais ion-sneíhane dunas are added 1,06 g (2¾ mmoB oí LAN m penden' ,'%o N e malvo ÍT^. baub is \nncd nt ambient temperatura tos 1 ^ hon*v (.hete,.are added. .Ooposse, '.,5 mí. oí oates and vi di m a iv camcd o ni ior 15 roinide.v \ here are ideo added. divpy'.me. 1.5 mL οι 5M sodmro h\anisado soiotion and Mñxing sx casxíed oíd fot 15 minute':. lite reaehon mixture is siséis ddoted whh MTBE and dichlot'omethane. Thf; stsvpestssots E thscn ídtered and (be preupiíate is wifcd #ith ΜΓΒΕ ara! dichlor<xnethap# lía? oíganse písase ís then dríed over MgSO.<, ñlfóred and ©pnedntraíed to dryness. Aíler rmsfsenoon K cbnmwtogtjph' ,« miseá ge! uging díddommaíhane and atnroosna-m-crimno) as eluanlx, Ose es peo red producá is obrainad in ilxdfe»» ol'an oiS. *H NMH ^uo MIí.,\ dnmo-ÜM 5 ppns ros va 3 1 h, / 37 (d, 2 i h,72 usmexohed pestk, 4Ηί,-ίίό42Η\Β(:ΐη,4.Μ^ 1 lo. ' ^s;,41h, 7.7 2ni.\BX( 7Hk 2,^ist Oh, 2.75 (í, 4 Η κ 2.2 (t. 2 11). 1.5/1.3 Csn. 4 i i), 114 {ATR}em';: v: -S02: 1333o 155 Το a aolunoTí of 1T0 p (3.62 ssanol) of ihe cosopound «híaijsnd ín Siep H: in 20 .mí., oí anUydrous mnihasisd (hese are added 2.0 g il® Plspoi), m poríicnB, of mapiesiüm; Sí'.nmuo'. Ihe iW',6 ss snrrtd so ihe psvevss>.'i.' of ultrasonnd fos Op hours Fhe reíií'ííon un-· ínrr o ihoo íihered. ilv soíkí ís v.ashed .-cOral times Mth meih uve. a-ml fue ídtmie is ronce mimad 10 drynesx. Alter puritlcaikm by chrematogniphy over siUcu gel usina, d v v >*1 ctl i v aro amo **a m Ον uno! (\ '.mom-, dx v\pix v frodiv ' 4s 'M* ?v :the,ftíTOof sa oil=<; *HlSMIÍ(4i)üMHAü«íao-dHíippm 'Míd.MOM 1 PMHuM ido. 3 !U 7 u, 2 1h. ' b (>:..? H\ LM U. 4 B). : ?5 tm. ' Hk 2.72 2 15 (M. 2 Ü), 2 ^ α 4 i i). 2 25 tt. 2 U), Lima 2 H). 1.45 i na 2 H) iKHFHlv·») x 'MO Ml· 'HMd.W i '0-0 C*· ÍÜ5 fíigh-resototím tttans spiVirometiy (E$l+-f¥iAfWR);
! mpmcsi! kan nl i 611>{\' O fM+H}‘caícuhted,20UV6l [M-Hlp mensurad MUMO
Preiumidcm Ti 1 A$KHJ-e|p®!rgifI»S-f I&amp;atdjlyit* l^^M-fetrabydrohmíídndiifieírihyílroichiftry*?
Step Λ: tert-Buty! {9oS}-4~oxaM4M¡iy¿mpiperaúml2J~cjmorpholme^caúm0MA i he -svinhesis oí dns o-numasd is km-oo u¡ die h lamían. tJ XfcJ i Mw 21112. ' \ MS7 íor fUe opposite cnantiomer). á'iep II- i^iShOcttihytiropipemzínof^,! 'C^mm'pht>iín-4'ime kydnh hhmíe A 4M soiution oí HOI in dmxane (5O mL. 2*10 mcnoi) O addad lo íhe eonipound aotdmt|1
{b#6V4*oxo-'OelídívdropspenyBtto[.M -cjmnrpímiáno8--c&amp;rhüxyUu.e (I L5 gy 46.0 nicooL \ vded o-m t a? ho n * κ sol on - m > m al rr*ntr en tx u x ío* „ hotm and Ihen a? 50-60"C íor 10 hours íhe sosainas ss tKeu erapomíeo io dmu ss I h¡. a-Orne is co-evaponued wilh díoxane (3 x 20 mi s and Ihen dned ia vacuo lo oblain íhe exporten eompoand in íhe tona oía salid. Ηϊ % μ» ροο %m . 5 2 íío - i'U ' íí\ : · ι ν*ο, ι Rk 5 2> - 3 ΐ ' ira. 2 Hk 3 5** i <Ό ί»κ 1 Ηκ 3J3 - 3όΜ pm ! )h, V*5 - 4ΛΜ pm ! Rk 4.02 * 4 >3 im, : i I). 4.45 - 4 S ' 05', I I S> 4; O (br 3 I h
SimSl terl~Butyt i'isrbtmytjl-ltltíA-tetrühyérm'iúqmn&amp;ññe-l-c&amp;fhexvíaíi* MK' -:3.90 n, 20,3 ñamo.!) ib edded to a xoiuüoq of the nompoond of Step 8 0.03 g, i 5.1 mmol). í .'kV|-01 i ier/dostos y íearbonyl]- 10(3,4“íci^,4'p'do4soqirOH>!Íne-3--i.'&amp;.n'so>í>1ic achí (4.0 ¿p ; ó.4 smnoh, Uíeün iassdne (5,.0 mL, 57,4 mnsol) usía HOBi (2.72 y. 20.1 m«5ol) in diehlowmethane :15(^ m! ». The tnM^í¡i#fed-::M :tóbip.t (^peratiire fe 2:1 Itiéiim.· sN aquoous !K1 somsion (8)5 mí.) d added and the precipitóte fornied la iasemd oíYusing <' 8 <.»mo Onsr,.i I se rhe\e, oo i Orale am vmise,,. 5 do χμ* oín p, · o exea·. v« ^íth d\ohxo5e,etham :3 ^ 00 mt 5 1 he eombuvd orejen' pha\e° me v\.í-d'-ei \*nh v\ aq acema HCÍ soknkat (35 mi..), then nith aqneous 5 % pot asaran 5 hkiabsmnH: sohnio.55 t3 \ 35 mi.) and fm&amp;tly with 'hrine (35 ?«L). fbe?^fg|n;%:::||ha§®-%#||d m$x Na>S€B, t%re# and toen oosmenmoed antier redoeed pseyoire. The produet m porítkd by chromaiügraphy over Mhea geí -.s.sing ethy! acétate ajad heptarie as eloams to obtaks the expected eomponmi Id o he íorm oí a so i id. %ίρΐ»<400·|ί^,€1Ι«2·.|3):«.ρρι«ί:Ι·38 ~ 107 (u, 9 H}> 2.39-- 2J9 ím, 2 Vi), 2 JO - 3.34 tm< i Hk 0 (4 - k7ts 505. 2 i ík i 90 - 4 dk (ΠΚ i IR. 4.(K* . 4 2> t?5k 2 Rk 4.3(4- < oO un. 5 Uk 5.30 - y 37 (05. Uik ' 0.1 . ; 34 (m, 4 m $m? ík (X^3^{9aS,hÜi^h}HÍ^Ípermfmii2J^m^wiM^ímeikyÍfh2J>4~ i mmkydmimg iim&amp;íin^MkpármñhrM^ 4M Hí';i sohaioií Is5 dioxíme (45 ;55Í., i 50 5555550!) 555 takteá to (he compoood of Ssep C 8> h g, 40,(4 O5O50S) eo-oSed 55i asi ice i<atb. Í'ho aü\pe5í.Moi5 d Sben bdrrtvl al ,anb'em mmpar ,0ae tor ' ^ Sióurs abd (lien 51 is e^pmao o ιο dmasb, 5 ia tas J v .0 as\'5 ed witb ΜΓΒ12 and then dfksá /0 mcm. The solid tlseraby obtained is suspended in :MlAydmféían;( ihé?5diJ,!i'RspsO p5 79.1 ns5MOÍ|4s added, l'he anappnJda ia 'f'Ouxed }, r ** s \«'ΐ v ,v t5' .he>5 00¾ , e tn mi -.-1 \ ih 3*5 aiei (' 'ail > 5 ovn iddoi! o%*: a pednd 1,0' " osínates J.íter I1 3 (joiirs. 2N eapíee-as vOiiim hsdroHñie mOuijou :o mi 5 ia hL \'Λ o evam <\uií\ 0 1 ? mal % t chkM ' g ? a >d \ < 50
|;11 g) are added 0.5 timas iaier, The .suspensión is filiered over C'eHte and rinatsd witb anOndoU <' \ ** eV > U\ ! h au ^umeumovd «<' daor^ ) he ms thevH bfeMided Id dissfsiwd 1». Mtpti OSO ΜΛ The resúümg solution-ds ülfcsred and the -filtróte ioneeÉhváed,· 11 »o a >xlue 'x> Oss-nK^ m oeih,ei«4 xoo mí 1 and Hita 4\1 ΗΠ snlutloo to dv\ane vJY m.U a added i de vduüen λ boa te i v 4-í'V and u\\<teO o ah aenvated x «.«reo.d (0 no ^ asi - m ng ít» , bou? i e ^ ¡νλ1 ιμο<ι ?\ iohned ovo (. v, go " d ar sd wúh warrn melhanol. The fíltrate ss ooncentmted umií the produe? a taris r.o erystalhse out. CrnaaíOsatmn is aliorved to continué for 16 houfs ai amblen! temperaiure, The so!k! ohtatned ts itltered off and Hasta! wifb a mistare of 2-prnpanoí Sll'Bh -he? O s h> τη! ¡, and then vúth MFRil (2 x JO mi T Altee drylng, tlte especiad eompound n? ahormad lH NMR (400 MHx, D>0> <5 pprn: 2.28 ~ 2.44 <m. J Ti). 2.74 - 3.00 <m, 4 H>„ 3.08 - 3.27 (m, 3 HA 3.2? - 3,42 (m, J HA 3.43 - 3.56 fin. 2 !)). 3om - 3.60 tm. 2 Ii>, 3.76 - 3.6-3 2 H). 2 Η), 4.35 · 4.50 (na 2 Η), 7.20 -- 7.41 (ai 4 Η) nC NMR {i00 Mtte, DjO) δ ppm; 20.00, 44.58, 50.30, 51,08, $ΙΛ\Μ^»ΜΜΜΜε 61,60, 64.61, 66.37, 127.14, 127.7!, .128.00, 128.77, 120.55. I3U5 MS RTSl y, [ΊνΗΒΓ 288 16 iVcrtaratien 80 ¢3.81-5.(1 -Os a-fc-atm^írof 3 J j hept-n-> fmeifp!)·! J,5,4-teírah>dro--
StepA: {3$h3~{hh'h<t!h<íh} !¡ tth'íi{iiphenví¡\tíÍpki>bVÍi~l JJJ'-iMraJmírii- is&amp;quimtíMá
The compartid oí Stop .Λ ai' Prepáranos 6: ¢4.0 g: 8.48 rnmol) ir? acetona? lie i 10 nd \ is placed in a .'7-ml mu revene labe and rhen \?>ahnn mdide sj.do g; 0,33 mmoh ?r added The reacüon mixture is heated íbr 5 ham.s al UKH’ using microsvaves {200 \Y). Ii a.; iheo rOtofed an?i ihe so Ud i a 'Λ-ashcd with díchloronieihaiie. 11 x* til ira le is evaporatcd h> dryn.essi and shen the residía la poríded h> cluaanatogmpísy over .Hhca ge! usina hepianc and eihyl acétate as claanhs. The fiüe compound is obtained le Ibeífdnn nf ao: o!h 5If NMR (400 M1!>% dmM>-TUO λ ppm: 7.o4 ül 2 ! B. 7 .¾ u!, 2 !!k 7.15*7 (nk 4 Uk 4 5 T5 (2d. 4 Ha, *!. 14 m. UIk 4 44 («,. 4 11k 4 44 lnk 4 Hk 4 51 ís, 3141 IR(ArR>cm*' ΒΟ)/v-U. 11- Miaos -607 &amp;tep B> ¡h-sO amuevo/? U/gy f / Mwathvií l ,.4,. 1,4- tefrfthyiiroteúqmnoiitie iíTK iOJm^Cd νΟΓ πικίι' ·> ' \·^ noH j '\XUed }' t*k ptu 4 U> SvO l'Ui\ Η ν’ !' x •aeetonitrite t50 m!.>. i><)\a»6>a¿aspiro|5.3 jheptane oxnlate 11.4i g; o.36 bu®!} added. íbiioued by ^safenaiS:||»6J: :.gi. 1! ,f vmmol). libe reaetíon- r^MtoíiÉlltostó· ibr 15 'aouis ai retlux The reaenm· mixturo i* íUunxd and *xuehcd wnh a-, ekmdríle, utv»! íhen ls ex'aporated k> drvo.ess. ! he emnpoand k; punhed by chromaíog-fí.min ave: Tüca ge! nving dichkmnoethane and ammmu.i-m-nk'T.agoI av e loares »he a de oontpoimd ;v ohtamod m iho fono .4 ,m od NMR ^ <0 Müa dmse-n< > ,*rm ' bK ,4 4 Uk '' (d 4 11), 5 !4^ rm. 4 Hk 4.*>3/4,2 iúát 2 11), 4.54 (i. 2 ñ). 3.75 <ui. i Uk 5.543.4/24)8 i3m, 4 Hk 2.7 (1, 2 Hk 2,68-2.58 un. 4. Hk 2.34 {o, 3 Hk 2.3!-4.24 um 2 !h !R CA*f»> cm *: 1333+ 1156 o -SH4
Sm C: (3ShS^-(>M>S^zaspiwf3^0^40iméÍ^Í^Ít2Jt4-td^^^kü^i^tí^^· The ?vK->ykued compound oí iHe afenvé Stop (1,3 g; 3.26 mmol} diasoived m 10. mi, oí mcthanol Powdered maguesium (633 sngy 26.01 mmol) is added. sn portions oí 160 mg averv 5 hourn, i iu. rvaeiion mixture K U-nod ín an ukrasound batir ihr i 4 lunas, h 6: then fílterod ovor Celite. washed wtth eopious amoimts oí meihunot and dren evaporated io duráis. Uro , mnp<mnd -u poma d bx ehromaíogrophy over aliara ge! using dichloromclhane and asunsMia-in-methanul o» eUmms. The compound is obiained tn ihe ínrm oí aa oh. *11 NMR C400 K!!L·., Uun><vd6) h pane 7.0j (m. 4 Uk 4,46 (l 4 ! h. 3,85 (s, 2 15), 3.51/5.05 (dd.2HT7.73 (0 2 Bk 4.61/2,4 (m. 4 Hk 2.4 (m. ! HK 2.4 (m, I H)
IRíATR)un'!: 3325 v N'H
Prepamlow 07 {3<S)^|(^iO^>ctehydr»ptper»3ừ|2,Í*cÍn)orphoÍi8»S^ylaa«thyiJ'' &amp;MP^PMtPMMPí í-e/m&amp;tph&amp;iipe^mpémpíitt^
Hk* aymhc.sd oí' db; convpoimd k dcscrihed m nV lúemíum {J \h-<i Chem 2012, 55, 5887). Símil $$aR)~Ovtahpi?&amp;pÍpemumÍljHÍmfirphif!m~4~ime kvifrifehforMe A 4M «olinion oí HC1 i» dioxa.no (59 mi., 154 rnmol) ¿s a<l(kd to ibo compoimd oíStep A i H 3 g. I-i i mmol). The soiutioa ie ílsen sOrred ai aíd§^l5t»mpeMarei:-lot :|-: ,he«* .aáÜ íHcn ?ι 4M soiulion of IfCl ín dioxaae (\2 mL, 4| ipo^ol} ksdpáípÉd: ThoidMiip |s Mined íor H> hours, Tí se soluimn ?s ihen evaporated to «ryness lo yield ihi ópipiod prodiscí in thc ibim ofa «olld. 2.79 2 43 (m, 2 H). 3,02 (id,#- 13.1, 2,7 Hv% 1 Mp ϊ 32 * ΐ 14 (v " m, * 5*í - ' o" vn> Mi# 80- 3.96 im, I Ih# % - 4.01 (m, 1 H), 4,05 iA8 c f 1 *.* H/. ' IR 448 idd../ !4.1, 3 < 11/, 1 113, 0 71 ιΝ % 1 IR 9.9| (br. f,:1||| ste»<t# carhmni'j-1 < 3,3,4 ~fetmhy{frinu}qmtuúme~2~carhoxytme EDC (5.17 g. 27.0 rornol) k addcd to &amp; solution of Iho compound oi'íhe ahoye Siep <4.0 g, 20,7 mmoíK i AS>2-{üt‘f‘^butox% $oarhonyi|-1,2.3,4-icíraliydixx.sogoínolínc· vcmho\yíic acid ib 04 g, 21.8 mmol). tiieilnbimínc (11-0 mi. 83 1 mmol) and HOBt <5 08 g* :2#0.mrM!Í:%iÁ^ld^iSéi1imé;(lii pif,}. Tbo miMurd ls Mrmáí-áb-ámfeietlT· UmpeváiMé} fm lo ívius 1M xiueoos Hv 1 -nAmum . Ό mi ' ^ add/J and tlie pmeípiulo tornad É hite red o*f o-mg a Hmtmer (.00x4 1 m plom m dio filma o are *o|vin<td. íbe otéame nhaso *' xa d.d voí ",fu , ' upa. * \ a s>n n > u vean -.opa >* >" 1«*?« conoíinmícd nmkr reJuted puxauíe ido oxidoe o; pm-abaorlxai outo khca gd <md is puriíied hy chmmatogmphy owt ssiióa gsf uaing eíhyl accíak -mé.:^ llopiaoe; as oina<í|s lo , ,o!d dio cvpccSf.'d oomgonn,i io, Uk tbmi of a solid 51I ®mk (400 ΜΗ* dm»Ml6) δ ppm: L25 - 1.58 (m, 9 »1.50 -- 2.?0 (m, 2 B\ 2.76 -3.25 («η, 3 11 >, ,U? - 3,76 (m, 2 » 3,92 - 4,50 (m, 5 H), 4,0« <8< 7 |¡y 4 66 (d, J - 15.6 Ib, I H). 476 - 5.28 (m, ΙΊΓ 0- 7.5l xa a «l· te¿n?k s 'éto is i njumoUn e iríkydr&amp;cfoíürtd# Λ 4M Mad-m «f }ΚΊ In dmxsate «34.0 mi, %1 mmols is added lo u soiuibn oí Ihc eumpoundof Btep C (8.00 g, 12,25 mmol) m dkhloromethane (25 mi..}. The mixture is stírrcd al amhient. tempemture for i 6 hoars and is then corteentraícd to drynens. The crude ptodúOL oWatned b added 10 a susjwnm -d i lAiü-, 7 9? g, 51.91 mnml) In ienahydrofiiran ?14o niLt. 11«e mixture i« reílnxM tmfü tha reaedon (mpplf&amp;red bv LC-Mm b complete and n is Jxn eooled to OT Wares 5 4 > mi } «x adden dtop,vsee MVt Mrring Sor i O «imittes, agüenos 2M sodium hydroxlde sedutíon (5 mL) 1 added dropwise. WufOi. 7 mi } ss ,¡g,dn .¡ddui ,06« ábmnje ior 1«) omunes Uto»o me frnalK Mded t el v «4 p} aral Na ¡SO, ¡12 g) altar stisrmg ior an adddkmal 10 minutas. S ha suspensión Is liltered ovar Calila and Iba 13ia artida msidna tbaraby oluained is disolved in metiiunoi (80 mil and then a 4M solador* ni HC1 In *11«¡xana (16.75 mi., 67.0 romo!) b added. The mixture Is sdrred til aniNent temperatura for 3 hours, and ihan eonmtmded to dryness. Tha residan is dissolved tu a mínimum «>f unmu meihanol (?0 mí,), and iban MI BE (3-5 mi.,) is addad, 7 he xolution is coolad al (Π3 fot IIo«f m an teasaold w$ter l^ciP#É^'P?odH9t precipítales 001. A tilda MIBT (2-* mí.) is again addad nnd iban the mixtura is alionad to stand tur a íUrtbsr t hour ai 0"C. The sodd ebtmned is fdiared ¡jxi a Uuchncr tonnel and dded m »iíf¡ Os Ce Id ido oxpsxted compound in tha form of a solíd, % MME 1400 MH* CD>OD) δ ppm: 2.65 (t, 1-41.1 Ib, 1 H), 274 - 2.97 (m, 4 IR 3.10 ul.í 12,5 ib. i U). 3 |9 «dd../ 170. 4 8 íb. Mil 3,2? - 7.ds 5 ib ) 6» - 5 74 nn, 2 » 3.82 · 4 16 (m, 4 Hl 4.45 »AB 4, / I5t« 1 Ιλ 2 ΠΤ 7.21 - 7 í<¡ (>u, 4 IB. UC NMR <100 MR?, CD.sOD 1 δ ppm: 30.21, 45.6?, 50.22, 51.99, 52.90, 53.58, 53.71, 59,25, 62.58, o5.30, 67.07, 12776, 123.40, 13.9.08, 120.36, 130 Í6. 131.95 MSiKSip jMtΗΓ 288,2
The thie miíipouná ís obiained stanlng fwiro 4-amIhophenol m II b »« tlu» mesuno *>t iMkiiEoie atííi ?*?»* .<* ♦ν,κΙ'ηκΛίιΦΜη* < Hiende í' ηοοιηνηοο *nb thc ριοΐοοο! 4e\cuhcd >r no h'naUnv ?3, Kmsgga u M, th^mh' Λ dentón" f 'ht’wsitw 4? f y 400? 4 a i o; *11 NMH; b Ο*?0 MI!' Jms> e<\ >00k) Μνρ^ fod, 41 Hn 4 M (<p * í
Slt-Ph' OVss.Oü SuUl·^ iMHOhOÍOn sdh S4 Λ l^H'^'Myd Ut: X ΛΟ ' 'noo MOOom'
Step B;h ^4~fter^Bfíiyii^n^th^iíyi¡fakrphcfnif~hm^kii~pyrazi>M^itm3e IV a vduimo of 50 § g ¡O I '' nv< s m she \"'npontsd of 8a p Λ n 03 se! o* esdedsoua totume there areisoceessively addod 29J g of sodium ím-bulylate (0.310 mol), 4,5$ gaf Ou'íijJxO, ^^.νο ;eforreo m m tnHdthen/MideoeaeefonektipaUadíumfOb >4 06 snsnoh, 4,8! g of 2-dwe7tóbulyt|diaspldno034\6Mrid.sopropy!-l JM.>tphmyl (OP! -mmo!| :ani' 12.8 mL of04-bmmoal-pethyl'4//'pyraMÍ© (0>I24 mol), I he hately j§: degassed «mler; ;.u goss tor 30 mimaos. and Osen reflused for 3 hours |f ss allmvcd lo cool The reaetson mixture ix eoncemrafed m drvness and Ihen iuken up in dichloromeflmac, fihered over í'dlie and ihcn eoncentnted lo dryness again. The ¡eaakse is then puriiWd b> ehivmtóognphv mes "diva ge! a^ng dtdikoometUasst and eth>i eetuue as elsiassf" 4' imvsdeíheéKpecled predóol^ m The &amp;no: of s Jíí NMR; δ (490 MHa: dmso-dé; 300k): ?.5S (se ü-L pyraroie); 7.23 (a, 1H, <om,mkK ?J8 (broad o, ÜL o.o4 sm 4Π arosnufse Ib) ' ?7 pe ~df. Cfh-pm/vk'V o'3* tv$R sí \i 0.12b "li 8' (01^4 ii{\ bfa IR; v KH , '075 cm - x At aed 3' \ O'"1 and Od2 > ό \ S O 5'< en x' Ss O . 8og cm'1. x’ “x'4 o 828, /,3 eís¡ Το a sqtoion of 12 g oí 4-andmopheíK?! (64.7 ramo!) in 200 mi. oí aceiomlrtie there are added. ai amblen?. tem.pe*aUm3, -6,7 .§. of imtda?.nle (97.0b ínmííl) and i?-7 g en ien h.ui\ Hdimeihx B$ü>5 dikaüe i)7 o4 amad) s he betas w -osoed .η o-t he 4 ίαηα 1 ?v m?0 water and exmaded wíth erken The organíc pha?-e ts tken dried over MgSO,s, thers ñhm'd and exv.noíased ío drym-ss. 1 ho erada pro ¡kan íheroby obláis sed 0¾ Ule sí pisrdkd in ünOniatogruphy o ver sillas gol n.-bsw jwmoknaxs eider and Jsehlntomedumc os eks on-< i ^ -ηΛ- |*rudne¡ :\ obus^ed m ihc -Van .,d ,¾ noxwk'í ‘11 NMR: .' ? *0ví\m dmodo í*H»ki N-b H¡N»k M'o ’H - otm.\o->k M pbesw,5 ?\04 sd, j!1 .ehis-xd. οΛ' uf 21! phenoxx \ o ?.M. H1 -animo. O.o5 o 011 ¡en-Mdx ib 0.15 ¡ ?, Μ1 d-en.dxx IR: v vníT 3403 em'*; ν;>.\τ: I W? esn': ^Mdirkifení JQis ’$w0M$00 5-1(4-1 \$en~ has íyh diaxniby Ι>ΒΙνΙ|ο xy} phenyl)aa»iMf~ 1 IT-Indido1 -carbniybde
The prccedure *s h accordanen wiih the procesa of Preparation r?¿pplacmg (he 4-bmrmy-* methxl 7* px? ^-!v ?¡ \».dl ss s b ηΉ s biomo Ui ?¡kkde 1 emboo lite
Hl NMR: b <400 küb; edmo-de. ΙΟΟΚϊ 7.85 (d. Ulr. 7.78 Oí. H1V. ?Λ4 id. 1H); 7 t<Ud. IHbek'osn. ΒΓηο^ι. 5Hk^>8(d. ití\ Uvas. 4H); ! 00^ 0|Γ>ι 0 2 o, oN? 5 ni mine
The pn>i.eduro ia in occouiancc wdb (he process orPreparadon l-muhxM '7-pua-f Hí. a*td ux Niep B by Abromo-! -smdhx b 1//omfele, ;Frefin^lnii;:llk A^^n^^Ret^K^^ttoyllsi^IInxylpheeyljhl-methyklll- imteel»5~mi0«e i he piocedim ?s \ ¡ aoo*xrdane<. *Hk fbe pnu cvs of Preparamos VI rephesnn: dxe4 -bmmo--i - s?sdbx!-1 /(- p> rn/'-it os^d ?n Siep B bj 5 -brom<í-1 - sises tn 1-! /Tindsszole. lie pBmbdnre h w aceoralsriisl -wiÉt Üa procer oí Preparatkm V\ reptad ng rhe í nnedvi M ¿f-fjuzok need w Step B hv 4 hmmo-2 4ht»M\v 1 «xdnlk o/enca
The- proctódure te m accordance wuh {he procer of Prepamiion Γ, repiaetng (he 4'brease ! -inethyl· I //-pv?;tóde «sed ín Síep B by 1 -bromo- l~f1uorohe«zene.
Prepar&amp;Bms fPB 4*Bm^toxy^/V»|ife^iykaiiili«e Ίο o eokdnm oí 4Byulro\^-plx‘o\b,müf«e \40 g; 1*0 «vnoB ín «een>nítrik‘ ní^ * diere are ndded 58 g oí i.'mí íh (178 mmed) and stkriog B camed onr tbr í5 mirones ai jáfÉbÍéÍtcÍ¡ÍKprat«r«. BenzyS bromkie (22.5 míe 178 mmoí) is ihen adádd dmpwyb 4ίκί ífei ábe madííbb míxíere is rBdluxedodbr 4 honre,. Aiter Itkerímg? 'áaslif: ^Mh acetonitrile, íhe Obrate ii e«e«ntratbil api pudOed iy :dtematography«ver slllyi jñí using pelrofceum ether a|É:^W*MÍ^ílÍ^btólií&amp; 'ftilÉ pAd is ihep«btelped id íbe form of a coíourfess IdíM, *11 SMK* 4 (kfO ΜΗ/; dm,m dt\ "0*40: ~.8ü po !!L NiK " Ί< nn, 2H <sr>h, ^ do im. 211, a¡\d, ?.*·* Ub α»Ί> v k (\, 211, anVh * 0' td, ,'B. ,«}]« \rt <m, 4H. iMo 6.70 (i, ÍHS arvíV, 5,OS ís, 2B, henzyh. ΪΚ: v; >NH: 5408 om'1
Prg»»rafk*i 9**:
The procedía* te m accordanee wuh fe proee^: oP Pmpsmdbn P« replacíng the 4-bromo·-Í -mcíhyb! /·/· p> ra/ole «sed ín Smp B by 4-hroraopyrídíne, IB; v -Nlb 520d and 25ud cm \ voSbO-' 402 cm v ν-λ'.. 820 om 5
Vréñ&amp;Müm ^l4^HÍ^ÍMÍIiílÍÍ^^iyll«wi»h<mvl4^Uftmaailm« í he proeedaK {?í moordanee vudi {he punv^s «* Ptvpomuon B*. sepUanag ihe d-bsumo ! -mtílíyM /f-py rabote «sed ín Step B by 1 -brome-4-fksorobenzene. I^paralion lV%i i W* py r rol» |2 pyrldl ss-S-sa as rae
The proceduré is m sccordanee wíth the procesa oí Prepesf^tíon Γ\ a'ptechíi ‘Μ***®*1*' %‘ímÉiyl-\HippñmM «sed 1« Stop B by $»brbJÍ&amp;í*H^yi4^ |tsÉihed hi M&amp;étámM wiih *,.ρκοίόοο! lYwn ih# Itiftgpaip ¿fówwEft&amp;fc 2<HJ3). ííti v.;-NH~: 327Έ cm‘!; v: aroamdc ·<>€·· mo i siles a 160$ cms IT^MSlÉMi© pyrteídiaTtertÉlte
The procedure i.s m accordanee xvíth ihe procesa of ITepaMÉiin F$ replacing I Hrnethyt~l tffryrazoie osed íti Step B by $-broirH>-2-meihoxypyri««<llne.
PreiMinaiBn 13°: ΛΜ4- díl%ydro4IApyr^lo|2J-#|p^ridlil»^spÍ^e 1 lúe promhuo is m actélámce vciflilhé pteess óhTi#páis!im 1", tpdaciog the 44ssoftH>' WüÉIT*! uáeá i*» Stop B te S ^ o sí lT2>3 -í^ih^dl ' í TT- py r r om > - f;23T?)pyrkfiae.
PrciMuytlott 14**? :^HÍ^4!^^^t^ÍÍ«ít^É^tH^l>ailyll9xyÍph«»yl>«l -*aÉlti$M$fe tepeid^iTS^siíMé 1 he procedure B i» aaéPfcée wllb ite proeessvpf Treparadon 1", tepiÉPi the 4-bromo-I 'meíhyl-! iTpy uu-oio used ir» te'p B h> "'Τϊίϋοο*! -anedn M /Op rv anuda* ole,
Tmmrattei ISA >%’iT4'‘{[ftí/'r-B«i>Udi«HOiyl3faÍI>l|»st>|pheny¡>»A^%“-iH»8eíl»5ri'" py π ri í »c-2 ?4 hí la mine 1 he procedíate is ha ucoordanee wuh the procesa of Preparados |% pgpiprrpg; iig láifp·’ 1 arete! íí/jhu i- j u η \Λ;’ *} * '>'> λ> Η Λ-dámete Ipv-adan 2-tarmnc
Fr«i>»ratte« 1$”í • \V»WNWVVstA«WViW^W\W,«V>''>''!>'>'>V VV . . ' V o.·.·. . . .·.>·. . > * #· * p;y mas id i 11 -%~a m i« e I Ki1 pnteeamv in 10 te> md.au r "db the proeev, ?0 Psvpm'.man *'' tepkteiu^ tm 4Φ?οϊ&amp;0 1 'nie^hyl-'lií-'pyra/oie twed i a Stop B by b^mmopyraaokn I .5mjp>:{'m'tidine. IR x O \''»i - C '« InUem' x ^ t- Φ tnr f3,4-h | py rkllsi" &amp;* a «díte
The prneedyjúis ituaeeordanee wstll ílxe procesa oí Prepamiípo I'meíhyl·! /ípyf&amp;zoie used in S*ep B hy (obtamed in uecordan*.e whh a prmeeol $r«mt Ote Híerature; VYO 200b 0525ó8 >aaí1mg trom Ckinr*) -p>m, r'-KmmR .md 2dmmtop»opm'edád> i H"|>y m> k- 2 - ¡es r bou 0 rite Síep A: 4~Bww-/,5-ifimethvl·lH^)??mie~2«€8r&amp;úmMÍé A solutam oí brómate ¡6.3K mi.,, 0.13 mol) m aceite actd (dü mi.) is added dropwise, wiih a o ed a. a du^no ¡ ev', tu a soktson of dtme*h\l 1 putote 2 ^athomune vi 5.0 g. 0 1 3 mojí m -¾^ rué aekt *. k*0 mi 3 I be bateh is stirred ai amhíent temperante iior 34 hours. 1 he t\ .ictio» mixture is titea poured into a heaker coimúníng 300 mk oí water. The .solid íormed is íi Iterad of? aml riosed with water. ít ti ;ika MysdlTed lh dkabommethane t.iOu na t and 5he mgdnie phast ts woshed x\ith hm\ drted over Nads(k, iiltered and enneeninued m vt*e*#o te yield the especiad prwiuct ín the íonn ofa ao!M,; JH NMK K Ik U d m m 3 2> tic 3 líb 0.0? pe * ΙΠ. o 74 i5s< m> A saluden oí the eompaund oí the abroe Step (1.5 g. 7.53 mrooíh 4-¡{*er/·· buivUtnK'savlssMknxj.aehK' t? 0.3 g. Obi mmolk soJ tan- (ir< hmlm < t.45 g. and iy 1 plr iogplí t?K3 - S I M^hsgp^í (0.0 g,. 0.30 :mmol) i o tolueno {20 mi) is ptstged wtih nitrogen, Tris{ dibermy i kieíteaceUmey dipn'iadnmgU! ?0.\S a. 0 η) mmcd! s\ udded, and Oten ihe tenedor. imxtore íx heutul a* 0iO unt b* s> <. >vn s *m den. on m. ed ϊ>ν i!t ) vC.sí ^ 5¾ -ΛΐηκΟ md Jv mixture ¡x nlloxved lo temro m umbtam 5 empeña uro. Waícc s?5mU is added and the m \t se ' x*uk\s t xxnh .taxi ex'tate 11 \ nd t IK < x*n <' yn e pn o o o xxuMvd xm i hurte »e id th.v co m nt'.uied i ee. » de producl is absorbed o uto siltea ge* and P'tr f ed bx % m>n\tmg ¡tony over sí!ten ge! .as ng ti tx Uv^ak .mb hept me í >., iianU ’ lu producl thereby obuhned is disxolved m heptane in the xvamt sime and ís aiknved to precipítate. whh sbrnmy at amhíen? temperatura, and then at ÓT. The salid is idfered olí ándiíhe opertdion is repeated on Ote fíltrate lo yíeid the expecíed compound in Oso lona of a sol id . *H NMK {400 Mil/. t'ÍK'ib? 0 ppm 0 lo ρ-. o He OV (V o uK 3 u (v * Mi. Too m. Hh.doKthr x, i !Ι\η44ρ{.,/ i Ih.ooP(d.,m S.7fi/.2H) Hí NMU 11dd MR/. nX'Ki 8 Ppm. 4 34. *#.72. 18 'hí. 33 80. 3104. KOI?. 114,3?. i 11 ?u, i lo.41. ! 30."3.1? i 5?, π { 23. 141.54. 148 37 MS(l'S!f}; (M-Mij* mensure»!: 143 3 4-f f4~i|^:entyÍ(dbaetbyI)siyÍloxy}pb«yl!amlnn^!»ntstbyT!il« pyrrede-2''C8rbx>nitriIe M<V4>imt4hyl!hrmaroíde (3^«d2|;aiidds4^i^feíeyc!óp,232|PPtéM (0,451 g. 4.3 mmol) are added to a solibios a! pyrrolmdmafhordtfik (4 g; 43.4 mmol} íít dimethyl carbonate iSbmL). ría- sokttion is stirrod at OOl; j'or 15 hmsrs, and is iban heated ni HOY? for 8 hmits. bhe rmxuue e; eceded lo ambient teinoeuinic. unú ihoo eihxl acétalo (30 mL! ia added The phases are aeparated and ihe organic phase ís xvastied vvilh water 13 x SO mi.) ¡md !N aqueons 14.3 soluüan Π s 80 mi ). The comhhied aqucuns pirases are extracte*.! tg en xvito otlo 1 a>. otate ig \ SO mi ι 1 he conmine»? ntganie phases are xxasbed xvhb brine í 1 s SO mi i. dru'd »tser MgStp. Olióte»! and e-meemr.tie»! m nh'no tu ohimn tb«r expeeted producl in thc tbnn of a liqiiid; I-i Μ * O Di 5 ppmt 1 o> 3 h¡ o j i 18 (m, 1 Π), 6.74 ~ 6.8.2 ρη. 1 ti)
Sfep B: ^Βίοηίΐί-ί-ίη^ίΗγΙ-ίΗψ^ηύί^^α^οηΗηϊέ ' «λ're o Λ b 7 v >4 0 irenoft *v ν*Λ td te a - 4s non »J 1* ονΦ> 1-1i* ήηο'ο* ' carhouitrile (3.7 g. 343) ransolj m Λ·.Φ-d; n unhyiíornsatsísde (ISO mi.}. The saíutíoo (¾ edrsed for 1S bmtrs ai ambiem íeroperature. Ánother amoustf oí ^dmsmosucesmoskie (3,0 g, i I tmmdt is usUed and the esterare h sbm.\t 0>r 3 htatfa. tilica C" g5 b Oseo .sddcd ^ÉW^.'lilIpnsíon k» ihen evaporufed to-dryness. t- p\H\d >>n a vT . ge' e*> n.» aiú tv p Ttes se pur de»' >w v ru<*m J^paphf pyerdíleé: ge stop odss ! d *.>t\ dta.it \> óblate , οον,αοό jodud ssf e '4 a sohd. :ν1ΐ:iNMrn;p(MI:Mbx, 0)0,1 oppm:(4,/- i.?.¾ I Mg 6 JO C4</--1.7 R¿„ I H.s Símt ...........17 cmlmmí^ím a soluium of 4-bromod~nsethyi~ i #>pym}W>2~earHonitnle (7.83 g, 15 3 nsasat) and 4,(( - w-bstt;.ídnaeshsl>.lhlk>\}ObUno (4.0K g, 18 3 atuso!) m toioedg (55 mL) 0.4 5 abnulgy, Sodium lert-hutyíñUi (2.42 g. Ht.4 usmolh tós{dteftx\1íde«eaeetode}l|pálaáiÉla|CVí (55$. aig, OJÉ 1»1) #il l-di-íert» bsíty{]tfa^.sgid5Kv-2\4(s>^4r({so|imtp>4biphesTiyi (255 mg. 0.0 tamo!) are Usen added us Use resgipa guxíure I he mixture i$ shrryd uoderptrggeav The suspensión M iban eookd \o «mbk'ní. tcmpef.aiure and Diteied over Celite, The- Celite eake b Osen oswed oí ti'5 eOt>! .teet.ee. ! >r f, i nato t- u>t.sb-.\! v. hh water and tben \s ¡lU hrtne Hw organic pisase b dried uver MgSO.i. tlben-d and eornsenírated ín vacuo. \ he proriuct is purifted twite h| eítóiiégmpfeg ovet siltea gei using eihyi acétate assd heptane as ehssat.\ ,'íthí ¡hen b'. trst.srattaa tn isegtaae to obtaín ího e\poeud produet so Üv- torra »»! .¾ soiid.
N'MR no»' MIL. CIH10 a jijan Ojo (a 5 \\\ O 9? («, 9 H\ ,V?3 ss, a Mu 6.5 7(0,,^ i'1 u * 1 m. o.tvi 6 #,o <r», i 1 i¡ 6,\) ss, 4 U\ NMK **< NMRiKOXM, UV ' «t^n *<8 i*;*,' " >w», 10* ' >*6 5 &amp;, 1’";' Π'> 5\ X 2«» o'\ t vi λ! I ' 1 ^ $ * MS (mi+)t ffvhHf 3.:8,25 ik*OBMMfptIfe*.r>: /ν*(4-|^"Βϋί> ¡{úmn'ihy ih%1]ox>-3-£hms-sw|*hen>S|-i-ouJIiyI- 1 //» pyraauM~«ii
Hk pjotxdose «'i m ,íví í'íikuvo xuth fiv proowd oí ptx'patsvtíou í', icplacmg thc 3 asainoghenol nsoí Itiltip .# lyS JiP^aetopteMl:, ln NMR (40u \1Ha dsovvd?» 3 ppm: 7,89 (hs. I H) 7,80 (m. 1 Hs, ? 24 sd. i ll>, 6 74 (dd, 1 ) i}, 6.52 ídd, ! H)s (>.42 fddd 1 H>, 3,76 (s. 3 H>> 0.92 ís, 0 11), 0,1 (<L 6 H) yifiaKltPlfe: \ "-'-.síi*A w\opov'4 o'' No.ssO'O'p' «Isovt.os h*mlr*A (5 00 o, )6,1 msnoD, 4-jumos-buíyídmt£thyfoU>1fexyjanühH> iHM g, 36,1 mmoi), sodñtm íeíAlmiylato 4.4,5!) ^ 4o.9 mmoh and 2-tíi“Sír/“bvítylphosphsn«*2,,4í>6í-irHsopropylbiph6nyi' (0,458 g, 1.08 tsssssís!) so sol ueste í 50 mi Λ i$ ptsrged vsth ssits'ofpns. ΓπΜdihen*ylideí^ae€t««e'K dipaiiadiunuO) {05>0 g, 1,08 muso!) N then added to the fisactíon mixture, and theo the Ixnch ts hoaled ai 569 íor f.5 Lasa lite mixture sx then ulloxved u» eoo! to asnbieM tempor.snsro Waíer ss mkled ssod tho ¡wscison ηόχηην b extruoloO vñíh othyl aceusie «8 x 20 sol i. i he ί,ν?>ι6πκ\) oípank* ρ)η$ΑΑ uso wushed witH bosta, and ílum esssscesstruk'd usidet redneed preosure, The crutle ptodues ss absorbed simo siliea ge! «mi punikst be vluorvt o5' -ur ihe. gcí us«¿ o»n «not > no SvlHmK o’uosim itepodnes obíainosl s- xb -χ,-Κ-α! so siso ^srssst \lssU; in he piano .asó ptVx'spíUH," xutü atinisig, ut ambson! u upcxousv í*** toa et 0 ! \vtei 6Hs 00¾ J s. xs.\vs».vi í.v>u ^«sro x'btuovd ns tho lamí of ít soísd, 5I1 NMR (460 ΜΠλ t 'ÍH'hl 5 ppm. 6 22 rv <> Hl, LOO v ü>. #» 6i (bs, A i ID, b 8! * 6.84 sm, 2 Hk o 84 - 6,89 lm, 2 if>, 7.17 - 7 r ssn. 2 i Π, 8 3o fdd, J - 5 i, 03> Ha ! ih 5V NMRdOü Mlh, ÍIK'b) o pprn: -4.29. 25.78, 109.Π, Ü4.73, 07.23, 124.17, ':' i k χ isi.45,imm MS[MOif 326.14 P.rmafMi.QK 22*0 ;V-|4-|íí?Fí"B«tyJííHmt*thyl)silyJJoxy|>|ie»y¡l-l“íetir»hyilrofwraft"3-yl· pyríixoM-aimiie 3'yl'pyra?o!c (U.7,8 g, 3.48 mrnot) dOsolveá in aíihydTOua Hdrahydro&amp;fan (20 mL> are tbr hogr al pibkaí temperalure in ihe presence oí sodíum ?ír/-biiiylat« ü.7 mi. 2fvf soíutioa b TÍO·'} and d’i{or«(2~d{-ít'r/-bu!yjphosphhio~2',4í,6,-tríi.sop«'>pyi-lJ>»bsphonvn[2-i2'aníuw4i>hpiicn>npafladsuoHH> *84 mg, 0.122 amad) i he reacíkm miMurr K' íbered iuor «Vio* and th«n evopontkd u.t druara.v 'The residuo js crysialhoed irom a mixture oThopiane/cthyl aeeta% fíitomd aná woáhid wOh hepane aííd íhen pidlied by .ehromatography (per sitie» gei issipg diilbt^utphano^md-nteítetot m to ypld ihe e\|H?cted produet, 5lt NMR OU0 VdO, .hoas-de. >00 k* 5 ppm 7j>: {d, líi, p>íü/ok*4l5k 7 20 id, Hk p>w.t>le-?Í.O. 7,26 (s. Hi NH). 6.68 id. 2\L Ardí), 6.64 (<f. 2H. Ár-H), 4.93 (m, ÍHsTKÍb 341}. 3.05 mi, iii THFb'H), 3 04 tm, íü. ΠΙΚΤΗ). 3 87 fm, 3Jffc: 10. T01'-5T!}. 2.33 (ni, Uí. Mil-40* ' (a ü!, UU -TÍO 09'i(S 40. !Bn), 0.12 (s, 611, Me) !8í v C -H: 285? cm'b v aromado: 1505 em'*: v Ni-C: 1249 cm'"* ilreitaraOoa 23 b 6al4-¡(/e»-ÍOipldiaK4liylBl¥l}oxy||di«si)1}aoiiao}pyryíaí;-2-MrhodÉiie 4'Aminophefto! ¡3 3 g. 30.2 samo!) íís added ¡o a soluíion oí 6-chloiopyndbe~2-· v.a ^-íaud. {1 ' ' \«n\d) tn 1 e\1n\ ’ p .obduvae (70 tul i Iho va,<v 'iu\w is heated ai 140- 1500.' fov 16 hours i» a scaied tlask. The btuch is the« cooled to amhiení tentperalure. budaeob í 3.4 g, 49.4 remolí asid mr;-haiv1 ξ di in¡·.· ihyhs dy i ehíoríde {7.6 g. 50.4 mrnoi) are sub^eptenlly added s»d ihe rniMure is sticred tbr 16 fcours ai amhieni ti inpeuttjre. s he mixtiee ^ díhacu o ih '-·>Λ\α i 1 es ,y¡i s .aul ibe nroducí is exuaeled vvhh \x.O{ i f4 \ 50 sol 3, Hu: «\gaaíc p4 .sa·^ a.n coíebtueo .aid wa-diCti wah \\ate> le \ 5(s rvi.s.
Ove huv Ü \ N9 λϊΙ ^ ího gha.se w tWn Oued ose* M**SO+, «h'nsl mU conceiuraicd in vacuo. The cr^e-.pr^ei! i pAá % #iiM£0graphy «ver lí'IM fÉ U';mg eih> Ϊ <«ws su, and hopuuu as e moK tt> «hu>n Ose míe piodnet. !I1 NMR i4O0 MH*. €DCÍ.1> <1 ijjpm: $¿20: (s, 6 II), 0.99 (s, 9 Η), i>74 (|á,:p“: ?,f, 4yt β§* 1 11),6.81 -6.88 im* i TI), 736 · 7.42 (p, 2 H), 2.74 ídd, fj, W 1¾ 1 II), 8.34 (dd,T - 4.9,. 1.9 H¿, I II) hsMR O 00 ΜΗ * ι IH R> i'' p\« -4 18 <$ η '* *ΐ 92 i! » v 116 5\ 79 >0, 123,20, 131.07, 14 1.67. 152.42. 15235, 155.51 (HS (ESI);: [Mvll|: 326.24 frfefóáratlaii 24)0 earhdpRtile
SimA$ N^~f{ieH~BuiyíémeíkylsUyl)oxylphenyÍ¡~2^kimopynmiM^mimBO 4~AmioophenoI (4.8 g, 80.6 mmol) avui tríeihyiaimae (18.6 mi.,, 133,4 ?smo!) are addetí lo a soiuhon oí .Td-dsddosOpynssmíms; (10.0 g, 67.1 raniol) m eihanoi 1150 m 1.,),16e reaclion snixmse i'.; heaied ai 150*0 los 14 hours In íí sealcd llask. The hatea is thcn cooítd lo amhkmt temperaiure and Ose vívese O evapunued oü in vacuo. Díchioromethan® :p0Í4®L) k aáded ts fc reslíly®, and (he® MidázéM (9.1 g, 133,7 mmslf ¡and imí:* (12.1 g, 80.3 mmol) ose uüdeü. I'he mixture is síined IVir fS :h0'tsim irtiitó Éptpmhní, The reacmm míxturc is dthued vdih water (200 mL). The pirases dos separaiod ahd Ose oréame phase is washed with bnsie s i x 100 mi ) 0 k she-s islrieil oTer MgSO^ íiiterécli #i|.; «OhOOOIralOd M vmikB The fhlhldd Is pdÉied hy éhfoiBdfegmpHymw slliea gei asisg elhyi ageíaie aKdJfepÉaee-m^hiiH^fBi obiaín a solfei 1 he halo is mmisutea in kepiane, tliuied oíTand imscd wuh hcpume to > iold Ose c\fxv?ed eompound in the ibrar oía solid. sIi NMR1400 MHz, dsnso-d®) 8 ppm; 0.22 (s, 6 H), 0.99 (s, 9 SI). 6.42 (d,/- 5.9 ÍVz. 1.13|j 8,hl (br. s, I 11), 6.85 ·· 6.90 (m j ÍÍ| 3,13 %J -1,7 lia, .3 Μ|1Τ!3 5.9 !k, 211)
Aifthydroux A?.<V-<límeíhylff>m«ai»ide Π0 mi:..} k placed ismkr niirogen !n a ílask and then iv , orw mi d ot \u\ - i ' > m "II ornad s\ added /mi „.\<m\!e s foh mi\ 4 o om\> ) s 4 \ .vMMísspWs5' m > γ ss κ \i La en m*^v s4tM mg 0 * n>nv*> me \ubv\.|uunh <uUW Vikyoo ¡s huNded nm-nfo foo .vdam s, fes 5 mmmr- and í.km thc reuetfon rondase w stírrod so 120'5C tnr 2 hola;- under a sntsogi'n atrooaphete i he reaethm rotutUored b\ !,C-MS, k complete. The rosxfore k eooled to stmbient temperatura and then water (I 5 mL) isl added ihereto. i he produet k extreeied wkh AeOh.t > 3 x 25 mi/ The orgaTsU: pisases are íMdihmaif Ééí wd&amp;hed wsth vvstfr p- m :|| mL|, and fien, whl hrine ( I n 2$ mL% Tte orgume phase is drted oves MgSO.*. tlUem) asid cosicessfrated m vacuo. The resido# sg píissí ee v . tm>ni<s.h>gro.pbv over ssk s gol α-·ηρ efovi acótate and bepnoo JvS&amp;hMHig: lo •ohtaut the cxpected eoropouná ¿si Ose forro ofa eohd ^lINMRCTkt ΜΙ-Ι^αΧΊ-ίϊ? npm, o. fog o Ι-κΟΟΥ*. ^hs.mhkJ » «Jfeim h*ít»-o‘'\,'t 'Hk-í'^^s ih,/ . tmK'ahiLv*' ομι?,πι' ,3t' SMR* 100 M!K UX'ipo ppm, 45!, i 8,10, 25 55, Ufo. 52, ! IS.foL 12 LOO, 135 22. 24 'L SY m is, la, Ud ^
Bnait»ritl.Í0»..2g><!.iV-l4~N/^Rttiyyia(iei;hvisllvlh>\v|plitt»vÍI~Í>trldemerí»mgthvS>l/#~
Sfgp A; 4 -Bromo-1 //-pyraeole (0411 g, 6 i .6 sismo!) k added id ffoXtens to a suspensión of Nal ! (40 54 id oi|| (2Ji: ¾ 70,8 mino!) in tetrahydroturan (00 mL) eoosed m un Le hath. Atler havisig taken away (he ke bufo. the xsdutmn k \!tm*»l at assdvust íemperature for 0.5 honra. |t i-'- agam eooied sn .m u.e /.sil· and whmsethane ·γ 0 nO Hts 3 rumo!'» sh ,uf!ed í he ^>íut¡on sx stssTed at asmnesu tí-superaturo thr L> hume, TLe .suspensión is the;·. coneeimated. The evaporutirm residuo b íÉlirálüÉl.%IÉ.MTBE:!(9i. ráL).:MÍ Éiteá. "Ihfe fdaate is e<mee«lrated m mam to ohtain Use especiad eompound sn the forro of an olí '» NMÜHtW Mil/. CIX'L) 0 ^«n 7, >7 na 1 th, 7.4 fos. 1 fii N~(4^gkm~MuB3élm0^MM)axyip^ imim 4-Bromo-1 -trkknuerknnedvvl··· 1 ¿7-py razóle dds g. 58.5 somol), 4-[(/erí«btslykhmefhyU síiyí to\v jaraiate ¡.m 5 g. b-1,o tro>oi) and toba,¡o i: 50 mi > aro adnon so a s0nOd ¡hae-vi ktd 0 s-k Uv \ '< al o ! n be;: .0--00 \\¡10 ' n \ou ios N vsnu v\ ,.u bien '-o, soso A >> -Sstyhtc U 1 4 g, irs i' moK .^immv-lHdvifdkopbrno-O'bibo' ηΊΕορκγο Udphessd (0 7? g, i Sí nssonl'- and o mdsbonm hücsseaeebesokbp dodsiaooo í \ .60 g. s.79 samo!) are svK.xc%>tvely added. The suspensión ts heaíed at H5°C for 1.5 hours, The reaefbn mixture ts-the π enoted tes ambsesu temperatura and water s270 mL) is adherí. The mixíoro is sslrntd Tor 10 minutes Γοΐκο vgo $.¾ n- dvn added and Tur s¡.'ppenOOst. <s tlUeso.l >m a \ rd <u Celht I k ~ή m \ o 'K 1' r-óe ¡a sopaokod de u oooas h tso i1' v\0 vEO Os cOmi uceUne (3 x .200 mL}, The combined argente pisases are drkd over Na;S€6 asad LHered. bilie.s ¡ '0 g ¿v addod O1 the írhsate as ¡ i ti se kdeh .s eusgorauv ¡o dsvsa^N i no produü is psrst.bed b\ ehromnlographv ovos siítea gol usmg osos i sseetae. and Ueptane as eitatms ! he t's.nb'Oi ohtattVvl ks r^o'^sUtOs-ed srom heptaoo (80 "Ί > lo oblaos toe oxpeoted comportad *H VMRAOO \í|Β\ΟΒΠ<}6 ppm, 0 J p ^ o I i), 0e> ’ {s,b| ] g 4 U> (V 1 Hk od! -6,71 (tu, 4 H). 7,25 bg l Hh 7.36 is, i H) nC NMR (100 MMx, CDC:h) δ ppsrr. *4.37, 18.28, 25.86, 38,6? isepb, bOm - 21 0 Hd, 115 í 2, 120 72. 122.7o, 12-0d2, 174,74, 14 i .0?, 148,4 * MS (ESI): fMHÍ.f 307,08 JPreuaratíon Mn? A6|4-|/e^-Bnívl{dsHíeíhyÍ)s%l|oxypltettfl|7|^osíe|8«*3^1H7l6· pymmb4~imnm
Sm> A; 4-Bmm&amp;~I-fmetmt-3~yt)~71i-pymz&amp;fó 4-ftrosno· iopvmeóle ¡Lid g, 10.7 somol} i-' drsseived m anh\da>us dimotltNliosmanstdv (15 tul,). 3-Bromoos.i.4ane (2,0 g. 14.6 snmol} and eaesiom-.carbonate {4,7 g, 14 mmo!) aro sssccessively suíded theroto. T he reaetion mixture is heateé:¿ur # a sefledi
OasL At siso etul of Ose waetion, the a>Kom is ovatHtrjticd nii'm mem aod Ose residuo ss i'uríbed ·". », hmsm.uogt,mh\ osos silkagei us'atg oichh^rosnothaTSe ceniaiomg díothsUuuíno. and methassíd as da;¡na to oieid the oxpocsoá coropousu!. iÉldl* N-Í4^tert-Bufyi(dimefhyí}.iÍÍyÍ^oxyphmyÍM^0Metm^yí)^iH-pyrmoM^(mme 4«|/».íí-But^iiüÍ5ivíhyh^lyijox>iauhiic (1.5 g. 7.2 notad) and 4-bu>moi-(οχοηηΆ-).A orando »1 '- '< ? rotad) dimohed m anoidums totraksdrcdnntn V-Ss sol » aro stnxed kv 3 bours ai ambient tenspendure lit !:IÜ#Iprcsieoce of sodiunt /er/4}mylate «3.7 mU 2M sklutíou in ΊΤΗ') and chioroí,2-di>tó#%^dpUospliiuo*2\4\6,“irikopropyl*Í¿l:!¿bíphe»yf)(2«' f2»umimK4hy!}phenYljpaíUiditsm(íf) i 101 mg. 0.145 mmoi}. The eeacdon mixture is i'dtered ove>- i.Vine and theo t. s. apunateU tu dryoe.va lito retador A puñtied hs «.htontao sgrafdn ítvir ilestpl Assing;::É4blóróMÉIJ^ Joniaiañi^ dietbyiarnine and ethyl áeetiie as situante in yieid Ote óxfptei#rüdm 'Ü'S^IR, JttXlM Imv d<> ox \A mm ' <í m Π p^" >n. ΆΚ ' tOiv'h pymatle-rHh 7.34 (hr s, IA \m o.A) id, 2H. As H \ o tó yí, :H. v-íl}, 5. A ¡fti Ui oxAonrg 4 £0 $p% 4Ht oxctaneg 0 03 ¡s. olí, !Rug 0 1 m\ ed 0 Me* ÍRt v: C-H: 2V5$ ero'5; aromstie: l505e«s'*; Si-€; KD7 c«t"
EimmlMIlilla. Mistura ni' /V-{4-1 |»butyd(di«tb>4}$lfed|ttsy}pben>1V1 dOnt'tfeyklií»pya®é ΛΜ4*1f/e/í«feutyl(dimeihyi)stiyIjoxyJphetsyI>43* $im A: Mixture pyrazote l:.o· a suspmtadtt «A \ate no0,, m un *0 3 'i\»u»h 4t tetroln tu orinan {ISO ntl) íhere la added, at: Í0A3, 4'brorno-3-rocthyl·I#'pyra/olc disolved in 15 mi. oí tetrahydroturan dspwteig Over 15 minutes. .Aftar stirriog sor 40 minutes at amhíem temperarme, ktdorotHhana (0.45 rota 7.45 mmüll ts addsrd dropwism and then tbe reactto» mixture ia sti m;d ovemight. Aíler addina water. Ote reactiva mío ano $s evaporaied and taken «p in diehloromethat».* The ropmk phsuo s\ sepas ovd oít'mtd Usted mor MaSO^. ¡iitereU and conceutrated a - <iry««ss, The resida* is punited by chromatography over ai lien ge! k> yieid a mixture ,4 tito ntk ^.osnprsatds ¡4-lxomoA. t-<insmi1.n l-pxs.molt· and 44>mmo· i A inmethyi p>saoote seípoctiooh te :t oten ot'4:t>).
Abromo' f£-pyra/.ote; 5¡.| IMMI4 ?500 Mi iv\ dnv>o-,jO'i y pp?n 1 45 pv I IIV. 5 Ά (v * H\ ? 22 pa 4 IO ^roeie-l^'dímrtfevl·1 W'-pj'ratEok: *f| NMR (500 MH'a dmso-dbj δ ppm: ?.8Ι%: 1 H)> 4<74|e..J Ή», 100 üs, 4!|¡ -Stei> B¿ Miente ^'S^-^fett^imf^iidhneíhyBsíMiaxy^phem 11- yS-dímvthyi" ί H^rmuh1··· 4~wwm iWii ^''44-ifíerí-hmyí(íUmethyi}Jiifyiio\\ipih-‘nYl}~ tJ-tiwutfkyí-1¿l~pytinol~4-
The proeedure is m aceordance with :I ^;*eplésÉftg thc 4-btaPiófeír hy lié ípklore oCisomssrs -Iktm Sep A. A mixture oí- issmes Ina íutso ol'4.4 le ohenned (reepeethd> Λ-14-: \h'H-bu\s Hdimeihyí isiK 1 jos> | phemdy1 ¿ - oral Λ'44* {|?trf*bmyHdimetbyl)s5Íyl|oxs4pheay! *-l .4' dim*ít.hyi~ 1 /7"pyraxoM~amine1.
Preparaste» 2ΗΆ A~j4-1rAt-ihstyi(dime t hn iValYitetyphrttyiHH^ctoproroM N~
PdAioM-ordl&amp;e
Sfep Ai
WiVrtVlWrtVrtW/^ A .*. y Λ y Λ 7 4-BrostHí-4//'-pyrazóle ;|1,76 g* 12ípsdnÉI):·M dlsibiyed in átilpirotts diínefeyil&amp;mtáMidé 1(15 mLj. Cyclopropvj bromnk* (2.7 mL, 36 mmoí) and eítesíum carbonate (7 S g„ 24 nrmol! are 5>ueee\¡?iveh udded Usetelo. The reacUon imvture i.s heatod tor i 5 honra >n Í60'-C in mMáidd tiask, Ai the eod oí thc reaction, :t$teb!vppt ss evaporáted 80Γ tn:WéM&amp; and tbe smidae is porííleá ir Ohromatography o ver siiica gel usmg heplane miú • dicblómmnfúanie. a* elaidía to ylélá ihe oxpected ta>mpphnd.: SB'f> B; Χ~$4-μ#Μ'ΒΜ\4(άΒη&amp;&amp;\4)ιιίβΒ&amp;γρΗΜ}4ίΊ*\%ψί&amp;ρ?ψνΒΗίφ}·?βζ<*ί-4~αΐΜΜ*ϊ i |íeíel4m\|pjin}cih>hat}>iios>andínetko4 e, 7.3 mmois and i-bronso-1~e>eiopropsI'I(/" p\razóle (i 4 g. **.3 nmsois dj^ohed m .míodoms tePaindmiuran í30 mí > are r-orred 6« 3 hours at ambiern lemperafure lo ;lhe presence ol smlinm /ren-butylMe (3.7 mL, 254 soluíion m THFí and ehloro(2-dí“fe>'í-h\uy!phosphínO“2\4'í6t4níso3pfopyi'í>r“bipheayI)l'2· (2-ηϊη?η''ο4^|>ρΗοον!|ρα!ίη4ίηηΗΐΙ) i 101 mg5 0 14o mmol). l'bc maimón nskinm is iiUercd x. o vü o'd uw to süyK's'. Uk í ¡du ' RomuuI h> xht ornato^; ao o o\ >.o -íSucu pe. osin^ hopooic at*d oih> 1 acvOue as duerna U vabt Use especie·.! p$\\!act *1! NMU rí.H) Μ!!.·« thnsíwitu 'UKl K) o j*«n. 7 f*I ts. IM. i*yf«A>Je-$*Hκ λ23 ^ üh pyt»mk-mk 7.22 tbr a, Ui NR), 6.65 {út 2Ht Ar-My 6.63 id, 2R Ar-li), 3-64 fro> W> a\» ioprop> i!\ \ u* i) 4 (?ο. 411, o 'Jt -HL íBu}> &amp;.Ü 6«, of-L Me* i 14; X- í'H 29 Mi em M mvoi.u.e I MU em \ 4m '; ' ' ^ cm's fíigh-rmofutkm mmx specítomeify (t'Sli}: b'mpineai fbnmsht; r¡$H:-?NM>St INhUj' cíicHbtod 3 MlMXU IM-Híf measuied: 330. i 969 ££g8aratí«m.2*BM 1x5-0¡feétfe^M%fcea^i»lj8«»]Nl^^i^|i^Ís^ir^ai|^Bf
The proeedure 6; in aeeoidanee vviih ihe pímoeoi oí Prqmmnoo 1", repiadng the butylíditneíhyl)ai!y!]<5Kyjan?line osed in Sfep B by aosiine. *11 MMU (400 MHz, dtnao-db* δ ppm: 7.fo ;s, 1 H). 7.05 (t 2 H), 6.79 is, 1 H), 6,6 (ni 3 H). 3.61 (S}3 HK 2,1 (s, 3 H) .Brepurotloit 30**: 1 * Meí IsykV* phem !~ 1 I kpy rroíoj2,3~6jpvrIdhod'Ooosu' I be prceedure is ín a<. m >2 mee whh Te ps «ñocoi ai v;op B oí ÍMcparadoo V u-hnp e>oone ami 5-HrooHvUTneth\ 1-1//-ρ>'ττ<οο| cM-oipxradoe i olnamcT m ocj. ordnnct o oh a pfoioeol ííomcite íxim&amp;immiMkhrmydex 60(4 h 865, 24MMU,
Mí» .ood ΜΗλ dom-díd o rrm: 8.1 ul ! 1Π. 7 9 \ \\ p ’ Γ -o 1 Hy ? -b id IIK ?.I? {i. 2 lh, 6T <d, 2 Uk 6,7 tu i \\k 0.36 sd, 1 )ü. 3.8 m, ' i f> il^Ragaliüt» 3Γ*; A^4~({&amp;YAB»tyI(dimetliyi)silyl|oxy|pbe»yI)-I~trye»^»|ttflfe|ik i he piocx'dure ss o> aeeotdaoee xxuh tbe provea oí iVeparation \'\ Tc¡4adop íce 4-N'<>oío-l -οη.ο6υο4 /,6|ora/ole uaed in Stop R by 5- bronso-1 - i tiideoleriorneíhyI s-· 1 /'/yn rrolof 3,3 A ipytidtne (obtamnd in accotdance a pro toe oí íVom toe 0 leonuro /ie/urocre/ou e>0(4}, 865, 2003, replafcing the tneíhy! iodide by tridetttemíed rodhyi iodide). lHNM« Ψ*'%ι1Κ 'v « Opee t> 0 u* , h " m t ' r ~(o ' f*\ t> ' # Uh 0.3 o.L i Ht> 0.95 ís, v H>, 0.15 (s, 6 N|
Pre&amp;arpOnn......3Γ4 Ή PHHm~Rtm id tmdhy Isíh inm }píie»> IJaminoH Tmieuteno- methy l· 1H - py r rwle-2-ca rbon sí ríle
Stm A; i' í'pfdmh'Mt*msthyi-t H-pynok'-J-airfremtrtft.* \>,dl *00 6 oí otH P *0 g. 6^ ' utntolt sn eo.spend d m auTmdrr4\n.m Γ. lo nd 'i >a ;vV 1 ^/-Pyrrok'-^'OaTboniinle (5 g, 54,3 m.mt>!) is added dropsvise over 10 minutes. "Ose renehou mKturc i-· Oten v-amuni ap to .mmiem íempetatate o\et Oj minutes. U ts again eooíed lo 0"C. mid then iodomelíume-í/? (10.23 g< 70 o mímd) ^-íidáedveibéo^gtída mixture i.x síhred ?or i o hours under ititrogen si ambiem iempemlum The seaetion mmnre is ibtíu exjtvMko temer teüuoed pse.^me, and ilion ethyl nocíate (200 mí.,1 and water (Pop mi i rao added Fho pitases me sepamted. ano the agueous pitase m extracte^ wiíh ethyí 'acame (2 x 200 snU< The combiued espante -pilases are washed wiih bridé (1 m XOmC.). dried over MgSO.j. iiltareá aui emteentMied m nseum The residí.®m puriíled by chcomaiograpliy over silica gd «sing ethyí acétale and hepiapéM eínapli T4te: feéinuavgre eumbmeJ and m. tporated m veo?,o to ohmm the espumen compotmd.
*H SMU ,400 Mil? t'ÍK 14 6 p-n rs ! ' íoJ. 1 2 ", ' 9 ; Hh u."N (.4b. * 1 T 40 He, i He 0 7‘» i4T * “ í,T 2,r» il¿. 1 üt 8$ep íL· 4~Bronte~i-'triU¿ut&amp;iwmihyMfí^¥rmie~2~cúrhún'H#ÍÍie' ^Brcmiosucciiúmtde (b.6E g, 3?.5 mmoi¡ is added te a soluüun of tile eumpouud ot' the abeve Step (4<09 g» 5ú5 ttuool) tn .V .V-ditnethylfurntanode t IBS od t. The m*!uUoo k xtírred £br If The resi díte Is: purfiéd by chromalngrapby ising ΛρΙ to obiain the expiíelmtíitpotMd in tita fonn of a soíki Ί! Ν'MR i OH* MMe, COCI Λ Λ |>pn*: o 's ul * ~ \ 'MI/, UkbdOol? ' Ι./ΙΙλ ί Η)
Stm C? dW-i&amp;m'iht&amp;idimeikyhiitffAtyip&amp;tntiÍatántil· l-tnd*m?rkm¿tk?l·/ti-
Nltrügea m óofefeled thremgh-a solmíon o«nixwd ol'ihc conipomui obtened ai the abovo Step ($.8$ g* 31.1 mnto1)« 4-(i#m~butyl4imeihyküyl)oxy)8iiUHne t8 g, 35J rmmd). sodinm tcrChulviate: (T88 g. 40,4 rmnoí) and 2-di-/er/-bttlylphoaphtm'-2^\6MntS6propylbtpbeny! (062 *ng> 1x56. triíMÍ} ia tolueno (260 mí.,4 tbr 5 mimUes. Ί ris(dil?eft/.yüdenoact'íonc)” OlpMladimidO) fldl g?, I;I6 tam-ñs (¾ ilion adiíed, The misture ís stirred for 1 hour ai K^C uoder mangón l'he -ayo n^on o. du\i >. ,a>C<3 m subí nt tempeteime, díliHvd wth dhyt avoune and Oitered ovar Cdhe. Mío t dito cuke i.a tfien rinsed whh eth>! acciaie, The fíluau. d washed \xnh ivaiot .(i itmesl and then briao tonoel íhv urgente piase is dried ovoy Ndy-Su*, i'dtcred and convvMnned Itr v.uuo. Πιο rnoduei is purílkd b> elminMfópaphy fw. diío&amp;: gel wtfng clhy! acétale and hepOingoas duant%: and Émn % Kk\v so gk.v-v d Onk'nx apnx η- n "mío m\t xaui os d«»K to oH<« i the o^ove^d conipound i a sho tumi oh o pnvtdu,
sü NMR (400 .MHz, CDCÍ.0 Sppra: 007 (s* 6 H), 0,98 (s, 9 U\ 4.96 (br. «, I H), 6.5? (d,./ · 1M6 1 IRodhJ 0 i Hilo 1 Mi 6 ’Odtr S.4IM ü<· NMR f 100 MIC. COCIO ¿ ppm: -4.38, 18.2C 25.83, 34,83, 102.96, 113.69, H3.7L \ \ \ 05s 1H >8, I M?5, i 2'» s 4 Hk 14$ 84 MSlKSI): ΐΜ-Η·ΙΓ 3314)9
FramiradOR .CVb: 4ν(|4ΜΙ6?β«ί¥ί# mothjl~5~metbyCll/“|>ymdv-2'WsOxiiMr¡lo $te» A; $~Metlivl-M-vvrr<fte~2^*teMm·
The cempouod is prepared i« oecordarpi WlíhOllg. potoco! desenbed in Hetemcycívs 2011, 62, 1503.
Siep 8: ί~Τ?Μ?Ηί£Π(}Μ0&amp;}!8$~ηι#&amp;χΙ~ίΗψ}τ?ύ&amp;·'2·<α^η&amp;ηί€ Λ sofistion ot ^-«hhK í»-K>mUHe *0 *d g. 3 8/ twnol) in MMdunethvk (ormannde- ix mi t \.oek\1?oí í. \uH hO so J> ( * s ' oiouv ibio’ poriiora. and 'ifie reacuña mixture i$ stirred ai 0&amp;C tbr 30 minutes. lodomethane-r^ (4i^ ¡O Ss «idv \ Sí O "3 jdt H>*U" md *u SOA i >3 MVUU \ V!t'u 4 ambioi3i tensperaiure smtsl Uso >ea<..tioo ss complete. !t 1¾ bsess ds'assed witkuotc i 30 nd.) arel cthyl acetato (lo sol.). lito phexos me sopamlcd atul tito oooem;-: pitase 0' o.oirai tod a iscxymd time ^<th odssi ϊοϊ - no ti'- 'tí i. s, Mu oosnd' aed όρο a so písaos aro rntabed vush water ti o oO mío, and then briste, ptd dried mm Na^SOs. Áfífer ; filtradon and conccntralmn uodcr reducen preasuro, ?be residuo is purifsed hy ehromatography owr siisca í;c! udnu othy 1 acct tío and iu*piano os oiuoraa i·5 su. id the o^pcctod o«>mpoutid m dse íorsts oí a solid. sII X\1R ai-Ou \p 1a OOCI·' o ppsv -.3' «o 3 H), N Ή sdd, / *o n U/, i H), 0.09 id, x ^ii/.iii' ;5|e|y£> 4-ΒΜΜ(>~ϊ-ίη0£ϋϊ£?ίίΜΜώχί~$~ίη#ί&amp;χί~ί£ΐφ}ψί·αί&amp;-2~£β?&amp;&amp;ΐίφ8β. Λ MfiytKni td broiitíno tO 1 “- 'ni . 3 w' mmofs m aoetic aeid (.1,5 mí ) ts added dnspwt^e M o, 'tnlutioTt oi'tho eomponnd Ohiasned m tbe alune Stop tí' 305 g, 3.-19 truno!) ti) aootio ;a id (5.S mh) prevuíusiy ot soled lo O'-'C. i He reacitos* snixlure is stírréd and g^íÉWÍ)e#‘^É&amp;iM Up tó sMitepf |.e*nfemium :o.^r:?a.«perí«á of 20 boüfs. The reáliíiM:mixtoréaspobreá litio vso?cr < ñ! sol ' and dto sstixture is topaos* J wol. J'ob!messa.lb,Uie (3 \ "*0 mí s. Uto organic písase is dried over íiíiercd and concéntrate! under reduced pressure lo yiekl liso ex poete d oompotsstd ilt the form oí a solid. *ÍÍ WÍK» «*s VRM ÍHlddppss- ' is '1)0 ms Hl 4^0-¡iíerí*BtityMiméfkyisihi}oxxjf$ñm^]'pMM&amp;$~í"trideiPi>riemeth?Í'$~8i«fáyt· iH^yrmk^2~Cúrñimiitíh‘ \ -itlS) t <.> mposo * i o eosap > s?oi oht *r >.d d t. ^ \n¡ ^up (' X* *>. 'O'! musí). (/^rf-bsttvdaissteÜsyláilyhnKyJattilitse (8.90 g. 59.8 mmoll» sndiuns /m-bu(y!aíe (4 35 g¡, 45.0 -m:mr> id and 2-di''r«rob«tyiphosphino-2'.4:>6,-iriisopmp>'ihipíte.nyl (0.44Í g. Η4 múkiO m aduesk t"O mío h purn d ndh mhogco. ! ; n(dshe?u> lidene\aeenmek ,dipiHadiumi§} pSil :|5 l\M mtm\) h added, and íktm the reactúm mixture is heated ai, fí5unid the reaetkm, tnotuiured b> ΠΓ is complete. 1 leming is empped and the mistare 5¾ eoalod m aminora fempetature. xV'ster (200 mi.; n; added and the mixture is eumeted xxeh ethx s ukekm d r ee-p (lie oombmcd Oiganse pluses ate xxadnd xxnh cas re, and íhen eoncentrate-d. The mate prodnet is purtlkd by chromatogrsphy over silica gd usmg < drvl anmue aun heptaoe as duerna Flu pmuuct ohíainen is dtssoixed su heptaur in the xxaan uta,te; n is dhmmd te preeipume ont m aodnent tempcrutnre and then at o t to ykld the exponte d prudact <n the lorm cu eñauad ^^IRhhH^m/.CIk InÓpprmO Ieie-6h\o%{s,9Hi 7 4 ja ^ i \\\ 0.4η - d.5 i í m 3 1 i y o »4 ts„ i Uy o o4 - o on -oy 2 U' !'5C NÜ1R {J00 Mí fe. CDCbjbppm; -437» 9,64, I8.26, 25.84, 3I.62 - 32.87 (m), 101,14, 11 I b\ 114.60. 116.33, 120.68.124 31. *31.13, I 11 11 148-18 MS(ES¡): IM-HJ· 345.13 íarí-BuíyÍídii«etliyl)díyl|ex>d|>ftimyl)am«*ftj"f-*nelhyl-4/¿f- pv í'attele-3-earboti átrík StepA: 5-.4 lo a suspensión::#f l-aieshyhd-iutru-íif-pytaanledaearhonítríle 12 g: 13.1 mmolj in m mixturo oí xxamt {J4 mi 1 and ethanol d 20 mt t títere are added IIC1 37 *n i 170 μ i } and iron filmes (5-1 g; 91 mmoí), Ihe reaction mixture Is .heated íór 5 houtx ut SO'-C. Aliar eoulmp toombK'dee'ivutuu henuetíOs a vmse n iPteicd IV u a <. tsu> kuu- ed id dryncss and ihen pnrified bs chóbnbthgtanhy over sil tea ge! using dicidotOhtótltane ani: efhyl acetato as ektants to yíeld the expeeted eompotm.d uvihe íorm oía solid, *H NM» (400 MUe d« 36} d ppm ->8^5 11), χ ’ .m, ' *h, . o n ' ih
Mm &amp;V mrámtkriíe ) he proeedure is in aevorduuce xvitl; the pro loe os oíStep B oí PreparfUtou V\ rcplacing íhe ΊΦγ*^«Λ' OrnetOs! s .f0p>niA4e hv nl~hn>rK^htnto\%K'Ví‘? OniyiviñetasOvina and Ose 4 buhlpiimednlKdyOnv pnnhne *n dw vompound dura Svp \ *it NM» (400 MI b\ Ofiiy*.a<ti O ppm: 8. .i 5 a;. ! m, M (0, '3 I i). 6. ?5 (vi i II), 0.4 s ps, 1 m. 4 ?< u>, Mh. oas «a *αΐκ o, is <v <» m
Preparado» 331 (i»orpholi8-4“Vl)í.'tlíy|]-U/-|»yrt‘»te«2«earb«»¡írlle
Hu; en vedme i - m acerad mor utlh ího procesé oí I 'repara! ion 33", teplacing (he iodomethane ·«(< in Siep 0 by 2 '(chl0'reetíiyi)Ti^|jpWÍ^ Itydrodrloíidé;, *H NMR (400..MIL·, dmso~dM $ ppnn 6.85 (s. 1 H). 6.75 (s: I ñ\ 6M H 3 í% l i 2 Hk 4,j A ~ H\ 1« »t. 4 H). 2 o A .1 1h 3 4 O. 4 Ih, M ís, ' Hk 03* (s, 4 Η)* 0 1 os. oi-h
Prestaratkm 361 yl}eítsoxy | pyrÍmiíl*rí-5-amhí«1'
Mil (1,0 g, 25.0 mrnol SO 5* m oth suspended in arÉydroos te4rah)^ift«!4sm^M:::Mí 0Ί’ in un ice baili imder argón, and then ImMplioiibneshmtol (2·-·?· gv 20o? iísiMrfe adddd irnpwise, Ihe ice h.nh k wi^draffip nnd íbe cnspunáon m sdtm! %r '1 ho«l#:PÍlif8Í' tempe?,nutv vil a no di bknv-psnmidiue (4,0 y, 20.7 mmnlí ts liten added al amblen! iemp&amp;m|}nv and tito ceacikm misture ..fe sllrred ibr lé hoiits at Sníuraled ayueous anrmoítutrn ehferide soktlksn (10 mío and water (10 mf.4 are added to lié j-eaetlon mixture: Ore pít fe ad)«áledhta 0 % adding satúrales.! «queras ^414(¾ mMurn. flte resuiílng saluden fe extracte! 3 timex with eihyl acetase, íhe nrgank pitase !s Oten waxhed with hruxe dried over MgSífe, and íhen evaparaied to dryness, lite expeeíed r.ompn*md precipítales oul hv rnear.s ol'tíu· addidoo m'petrofenm eOwr, *Η mm (400 MH-a dmso-db) S ppnv 8.75 <v 2!h, 4.4 (t 2 H), 3.$$ (0 4 H), 2,7 (1.. 2 Hy 145(1.4 11} IR :lí§ÍÍ2 v M?'v AAMíAr ,¾¾) _ §; ^(4,{$Ϊ€Η"ΒΜ?&amp;$Μ&amp;Η¥ί}$Η^ίΟΧχίρ&amp;Μ)'0-2~Ρ-{Μ&amp;^ίί8&amp;'4'·?ϊ)ιΗΗ&amp;.χ\'^ pytfmüiin~$~amm&amp;
Ibe pracedute is m MbdrdMie®:iffi tlm pmíieol !i r@flátíisg ;lfe 4-hvmo bmeím p i/ñpvr.omie bv tho eomponnd bom Stop A.
Jil NMR (400 ΜΠλ doisu-db) λ ppm: R 3 (a. 3 íh. 7 § ís, ! U\ (2d. 4 ID, 4.^ (i
3 H) 3.53 a 4ííf 2Ad. - II), 2,4o <*.4 U», 0 ^ (sMMh, 0 (s. b ÍD v 837 v -Sí-Mtf, 837 and ??S v -CI! Ar fimmratmrs 3?·*; 4~(f4~[{íe^BuíyUUmeíhyf$il>i)tm l|íiie«vl}amímip!,3-dimeíh>i”ltí-::fji$£rrek^2» ca Hb o» * t n le Sít'pΛ; 4-^¿ίϊη?Λ-ίρηφ-2~ϋΗ 1 -j/}-Vo,ierre·-'iMalpktmamuL·
Vhhmhm ilo o mí . 0 M molí «\ nodo»* «ner *.x'tmd oí 4 minutes to a adunen ai m&amp;v\ oh!o<\io >> I v o i ' nv3 m dsobMiomcdunc A"H) mí) oookd so au ice haih. Tdeth>·lamine f 24 ssL, 0JI «»| il:iiíifed and Alten ibi lolntion is stkrad al aniMerlO (empernare íor 1,33 hours. Aqueous 3N i ICI soluí ion (60 mi.) is added and die phases ara theo separaíed. The organie. pílase h washed wkh auorher ansouní of aqueous 3N BCÍ soluíion i 60 mí A and íhen wsth 3 3 a aquena*. xodiuro bie&amp;rbonate soksíkm í60 mí.), The organie písase is dried over NashOo, Slllered and eosscenmded to ohtain the expe&amp;íed ooíopoudd idibd iít3i of asolid. 5H AMR ( Hat MU a COTI*) 6 ppm: 3,43 (s, 5 ID, ÍA4 · i,63 ísti. 2 H). 4.50 Us, 1 H). 5.05 -5.22 tm,2)l>. 5,66 * 5.7¾ <m. IHk ? 31 (41./- 8.1 He, 2 l\), Ub{4,J - 8.Í Ha 2 H) 3Afíik>nj-2ooe4by1propono (20 mi, 0.20 mol) is addad over a peded of 5 minutes m a °uspeiBion of 4' motín iM ~t pi op-2 oo- S λ APtai/ene- í~süipíam.umdo (2? 4 g. o í 5 mol.) and pouvisíum carbonato (2b. t g, 0 20 molí m 5 Λ dmieihs íformanude i.Mo mí í cookd ni au se*' Yoh xi'v'í 2c ηο^ιοΊ"·, fhe msh>0"ovOu y cmod ai amKem n mpounuro ir' I b lenas, The suspensión ss conceotraied 10 dryness. \ fie residuo la iek.ee up in oihyl acótate loShml> and svíuct (Π0 nd,}. The apóceos pause is esíonned witls eihyl aeeiate (2 κ 50 mL). 1 he coirshjned organk pisases me washed succcssively with aqueous 2<N HCl· Ne'ui'on (v nil l 'uní?* v* \ o¡ wl * aqueous :5 5f··»:gmasseiooi hicatboiutle snlutioo O* rnl o uid miad) hrme , Ύ mi i, The on\om pitase is dced i>\> ' \a,SOe OhtríY' ard coneestimiod a> nhimi me m pconel componed ,n dio fono o? so oil ¡I1 NMR i400 Sillo. ilXYtb ppm I mi A s Oq 2 43 is, 5 iIV * ?0 iv 21h, 1 V - ' Ύ mu 2 Η V 4 87 / 25 Π Lo í U\ 5 04 « <¡ «*) im, i Ih. « 00 - 5 12 imMW> 4¾ - $3® (m, lllh 7,29(4,/-%! H%2f·!}, 7.71 m#-1.! H% 2 H)
Λ soiaiiod dTlhe upmpaipd isihdned iu Smp B (142 g, 87J iMno^ m laluene pSO dEíTis heated a; 50 Γ m ihe picaneo o? ij,' (n,\i2,4 OdsenedssIphem!>Oemídu/vhdncOdcieV
4|ehioro(phenylnHHhykmeK4nc>-e!o!ie^iphSÍ^hÍMWÉ^llOII: ((kstofosí eamlpT 2M geaerulmnl (150 mg 0.18 ornad) íor I hoor. .?.,.!4>iehk>ro-$.6-dicyano-/>•benxoqumone \H* I g„ >‘0.9 señad > m then added n; - >oo no; non, and Oic M'ho'HO se Ueskd si NU Y P>r 24 honra, The soba ion is íhUnep ovar rdue, and ihe h i ueie ñ; o oslo entra ied ¿#.> vacua, The próduéí II jwM^d:%:séfe«m8%v§ff{piy nver stliea gol usmg eihyl agp|a|e and heptapt as elisuius Se oMam íhe expeeíed componed o? ün icen efe oIuí *11 NMR (400 \Μλ CDí Id 5 ppm: 3.U2 td, i Unix. ) 14 2 50 5 llh t> og - ρ.Π
Ym> 11¾ ÓJS - 490 <ns> 1 H), Til ·- 7,02 im, I % 7,27 (d, 11% 2 Hí, 7,72 (d? J - H,4 I !>% 2 II)
Stm .i): 3»Μ*(&amp;ΦΜ4*ΜϋίΗφ€η&amp;η4κ»ξρ&amp;Μνύ- Íff~pvrmÍe-2~atrhünitriÍe
Ahimimum cidoiidc Π9.4 g. 0,15 mol) h added ah ai once fo a sohuioa οΓ:Τίη«5ίι>1··Ι··(4· nieihylhe{izenesidphonyiVl//--p\m>íc 03 0 g, 55,3 mmub m L2 diehluruetfsane (230 ruL) ai amhtt.iu icmpeiatuse liles \mrsug los 7u mmsikxs, s.>unngen bromide (il.íí gs o 0' t i i fded m pomxma «vera ^tiod id 2(» «nmítes \Uei l n hours. m addmonal uuouri of s.ns! b os sido < i Ύ g '\ ' t' mol) i^ udikal \0or diumg lot Π hme^ ut eofment temperansre. tl»e reacio si mixturo ss pmsred xhnvly uim a mixture oí djchloromedtane (300 sssl.) and water (dOO mi.) cooled 1.0 O' i.'. "Ihe nsuhing mmnmU I r hm s e- , iv me p( v av ^paoitu *d ¡ ,iu a--, \$ \V >~Λν v* J' d\ ¡k ά < >vvm s ' \ IM 'ni I h. combmtd organiv pisases ere waahed oiíh svmer s 1 x 150 md > and brme (Pe od s j &amp; orpssio ph ese 5¾ driod oser Ha^df M stUemd and cosieeo(r<«eJ o x.wo te eh.ms Oso uxp.eíed umnpssnssd m the tono otM sohd *H NMR Mou MI \¿, CLH'I 5 5 ppm: ; Dhs, > Hy L43 (a, .1 Is), o P (d, ? Ό üy, IHí, ? »' *'>(,') ds| Mí* \ Hronuvxucdniinidt * l .1 o a. o" 4 nsmoh ís added all oí once ns a assapenxson of d-medol'· P<4-me}hy]ben2enesídphonyii-'h¥»pym>íe“2'earboniínie (14.45 g, 55.6 mmol) m i¥,¿¥-dimethy í tormamicte (60 sol '» .o amhtent" tit&amp;pmtim The mixture ís stirred «t ambiení tempéralos ibr 29 hmrrs and ss í:Ü#ft eodlid m M lee feÁ. laliráteá bisulphite soluüon (00 mLk water (90 mL} and elhyí aeelate (250 mi,) are Oseo added, The phase* sis sepárete, h mú then· Ose Rimaos phase is extractes! with ethv! acetato (2 x 70 mío. The eosnbissed «rgasñe pisases are washed wssh 5 5« aqeeous posassómí bicarbonato soiuüon (Mi mí,), water i 3 x 00 mL). and íhen Oróse 13 x 90mL). The «irgame phase is drsed over Mobil?, ísltered and eonceniraled in vacuo, The crudo prnduet is rpnsifcd iig: ehmmatogmphy «ver silieagel oil^diinéne and hepiane as eínhnís (o nhtam Ose expedí: d eompmmd in (he fosan oía aoiid. TI mm (40s.s miz, COCI*} δ ppm; 2.13 OS. 3 IIK 2.45 <$, 3 Hk 7.38 (d,d- 8,4 m, 2 H)f 7,43 (s, ! ML 00*5 (d,,/ ::: 8.4 He. 2 H)
Step f.; 4~li?omo-IJaímtcíhrT/$í~pvmtL·'- 3~< m'homhih'
Pos s.V'S'esí Osdsxsssdc (5 g, «4 1 msisof) ís added all a( once ns a \uxpensson oí 4-0ronso~ Ln t'shx M-(4-sneüsy1benAne.sulpho?s>i)"í//'(s%rroIe'2waríx>sdirde p-1 ,ορ (> 7 mnsol) In snethanol (95 sssl.) eoosed using as, ice badr Ai)er 15 minutes, (he sn.speualoss ss sdrred ai ximhiesit kmiperalssre fus 17 lajera. 1 he meíhasusí is es aposated to dryness. I he esaporatkm seasduc ís taken ep sn IVÍIDL (25 mLlassd waslisd whb water (25 rnL). 1¾ arpsófíns phasp ís extr&amp;eted sviíh MTBF. {2 x 25 m!,!. I'h* eomhmed óigante phasca are dried ovcr Na¿SO.^ liitered aoíl eonceniraied ñ? vacuo, i fe·· residus· outasned u> dsssolvesí m ietsahydroHisasv ^ΟπΊ) a» J te noUuío \ · us λ "-te* an fedh Vdl ste fe ir *x s -fe fe 25 N '*mofe ' auded \ tu > os sses vdm ^ o s' 4 »st '8 ft s'sí'oI) ^ <te ateo' The piffehte is kj <eJ \ airfc \nt s 'ΐρο»#» o> * ^ hoi Ihe *~vsahxJ* "Un ' oxafesu >d\ jí\ü(\v I κ k'nua h' 5-¾.¾ *p > dschfernn'sethai;;; ano xvashed xvfes wates\ ihe uqneons pOi\o le eanseiod vafe oíehh-for¡'o*h,mc \ he oosxte.sxed orp;eae phavx are dned exí s >í,í>\0,, ufe-red and cnsv enírated <·; re· sn iu teiain she >.-\|xvted eumpcnsísd hs ihe h>rm ofe ioM, *11 NMlteteXIifefe'm’fefe'sppn* Tfefe MK * fe fe. ' II),ü. '-fe·', i H)
Sms G; j~{fé~¡¿é^~BuiyhiimYtáyhihihx^htmyíhmmt>}^J"dimethy!~n^ym>k'~2~ afrbtwítrite 4-Btomo-l J-dínutest-i ^psuole^cmK'niud* {2,02 g, 10.2 smnolj ahd 4-|(/awfe buiyidhite*hy Isih 1 )oxy]aaifine 41 g. 15.3 mfrrfpdre dfeÉhted lo totehé {40 mLp The soíution fe deg&amp;ssed with «itrogen sor 10 rolmfe., Jodfum tefelilykíe (1.1 8 g. 12.2 rumo!), 2-cü-te/-Hutvlphosphmo'd'^'^-iriísopropylbíphmyl.(0J7 g. 0.41 mmoi) and rriNfdihv a, \fefesa.vi, eeuvhhpsshaüsuso·us sO 18? a. 0.2 ®πκ>1) are thee &amp;dded, l'he mexiure fehsteed ai 100SC for 30 minutes asid ihen eooled tu arobknt: (SCf.SÉi.i aad Cellte {0 gl aro ndded The suspensión fe fHieml oser Catee. and ihe fíltrate le diluted xxUh fe 1 fe i Ihe phase? ate srpeuaod asa* -he a.pseous phase ss sxüaesed vxísh ΚΊ ΓΒΚ s2 x 5ü efe ^ Ihe contened sjuyusse phases ase dried o ser No-Mfe and fsltesmt. Ihe resate fe pufefied fe «teosmuogrsipfex uves sdiea gol rssusg eüsx¡ metate aud hopíasie as ehsassts Ibüosvod bv \'h'Sv uh.sso eh;on\u.'g?aph\ «ms^· mwth.as.d anémmor as- eluafíts.. íhsg prsHhscs obiasueíl fe Ivophiílscd to pruvide ihe expeuted eompsxund su ihe forro í'f a solid. fll NMR (400 MUz, {.'ÜCfe) d ppm: 045 {s, 6 Ilp 0.98 is. 9 H), 2.05 (s} 3 H)2lP C% ^ 1Π. 4 fcO {bt vid 0 * 55 · o '* ? (Oí. 3 I fe o.pe ρ,ρο 0¾¾. '* H) i4í \'XtB v Ofe Xdh *ΊΜΊ * a porí, -4 ϊ 4 a„\ ÍP2\ H 84. «s Nfe HV Sfe . ' fe fe 120 T\ 13fe'126 ’ 12- ^fe 1 iu 08. {48 methyl-í j7-|n r s-¡ s le »2 - e a r bon! Irs le
The. ps'eeedure ís'Ir aecordane-e whh the |>ró0m.i>|-'Prep8jraUoírJi'??· fepáéliifAe; bdometheaie-dj in Sttp i !>y mdsaeilhmm Ή NMR (406 Mil;, dmso-*J(0 6 ppns: 6 85 (s, 1 11¡, 6.75 (s, i Hy 6.6/6.5 <2cl, 4 Π), 4 (quad, 2 !!). 3.1 {&amp;, i tíh O <$, 3 i I), 0.9 ís, O Hk 0.1 <*. o H)
Pref»ar»tl»3.3%”: ^|4»|tf^B«tyKdimfthv>)aiÍvM»ai:yfthmBy|j~2.^ihnxy.pyriiWl«1iii.«u atóre &amp;im Ai S~Ermmi~2^íhmy^mrimk(Ím 5-Bromo»2-chloro-pyTlmi<im«í (S.Ci g5 25. mmol) i<s dsssolved m «thanoí (55 mi.,} and sodium eihyíaie (1.81 g, 26.6 mmoí) ís added m porhons, The reaedon mixture is .stmed fia 15 hours .n anshsem nnnperatsne. When she mus. Pon is ampíete. ihe -íohent is m qmoned otY. water (300 mí.) ^ adiietl, and shen the restasen mixture Ls extractad vbth dlehiootmeshane {2 x lOOsid.) The irganlo phase ts dried ovar Na¿SO.}, and tben esapooned fv dr<oevt sovsüd 8~hsosrHí-5 ume\\~p\dssndsne, 37¡p.* Bi N-¡4"ftert~But¥ifdímetk}4tiihifanpkemfy2*eih&amp;xv-pvrimidÍti~5~iimme 4-j/i.I?'/--Buíyí(dunethyi)sd)4]oxyauíline i 1.8 g, 8 snsnoU and 5-bn>sno-3“eího.\V'pyrimsdíne (1,6 g. S oimo!} .'tó'iwd hs anií\díoa\ teírsth) drotuiass i ío mf) aiv. anned ibr 1 hovr ai snnNasi tempes ams-e m the nresence «d ^-dmnt íapbunhse (4 mi, 2M Kdutsmt m ΠΙΡ) and t;hiom{2-dí-í<rí-buty!pht.>sphiníí"2i,4’.6,~ínistíps-oipyí''I J!-b!phenyll|’2-(2"atniriGethyip jdteuyjlpnlíadmmdh 1111 rag. ojo m¡stolT 1 he rene? i en mixture sa lihesed oves s. vine and Oten evapora (ed to dryness, The jetóte is (riturated in heos ene to obtuso the e-xpected eompoond alíer fi I (rali o«. ífu NMR (500 MU/. dmsO'db, 100 K) o ppm: 8.31 s.s» 2H, pynnnáme-% 7r8C) C% íü, MH). 6.73 id, 2H. Ardí), 6 68 id. 2H, Ar-Hh 4.26 íq, 2H. Qi >011.0, 1,31 (t, 3H, CBrCftk 0.94 (g 91i. ''Be·. ¡T18 6H, Me) IHt v: anomitíe- i >04 >..m SnC i >4?em'si C O-C- 1057 ·su"! freimra.tmn 40**; reof-Bmt I Ι>-|Β -niethx 1-1 /Apymdnj j $n r«H« -5»j i)amt««!' py rkfisi"3o 1 carbonate Síi'p .-I.' &amp;~Bri>ttmpyrítiith$<'yí ierbhuiyi mrh&amp;neíe lo o iükMx oí s i. o ! bio*í o "< \b t ' ul \2\ < mmoB ^ íj > s' μ oí ds /o* dicarbonato i34 5 mmol) in 50 mi. oi íetmhydrofusan títere ¡s added íU8 g oí 4“ don, dwhsn.soopxrnhae .14 mnoB, He oumuh1 κϊ sí «red .4 amkenl tempeudarc tH b hoors and then concéntralo! I he residue ohtamcd is dissolved in a mixtura of elhyi eihe* íkiil water. Ato- separaron oí the pitases* thc xeparated~üíf organic phase ia dried «svfí MgSO.; and coneenínued n> dryrnws. The ufe produci is ohuuned in ihe íorm of a solid wltldi es used in (he Ovxt Stop wuhoni hemg otherwiae pmiíted. *H MIR (4db MU dmso-df) h rpnr n i ts, 1 H \ ? 7| (s, 2 tl\ 1.5 (x, '5 i il earh&amp;new lo a xoíutlon of 2,74 g of the compound obiainod i« Siep A (10.2 mmol) in !5 iit of adueñe and i 5 rol. of teuahydroíurán íhere of sodio») fer/%ntylate 113.3 *nmol\ and toen dio batel· ís st irred arder argón ior 15 ininotex. i hete rs then added 0.3* g of paíindium cafalysí <0,5 mmet). fbe reaeüon mixture is then stítred at ambíem lempennure íor Ib koutx and then Bife red, 1 he Obrate is enneentrated and taken up in a mixture of díchioremethane and water 1 he óiganle pitase ia separated off aad then washed wiih water, dríetí «Veif:^1g80%eíiiigkd and conoetnrated to dryness. The erude produdt íltereby obutmed is purided by ehromaniígraphy over xilina gel usina dichlormnethann and ethyí acetato as oinnms, The tesidue is tben taken up in a mínimum of Isopsopjl atbet íke solid then obtainod >s di te red off, wttsbed wkh ether and then dried. The tille produet is obtained in the. hasn ot a solid, which is subsequcittly used withont being oíhsrwise pariied.
*U NMK idtdl Mfhr, dmwndb) 5 ppm O ís. i B), 835 vv>du i íft. S3 twd, 1 IB, $ (wd. 1 ID, 7.45 idd. i Bu ? 45 (wd, 1 IB, n.K ¡d, 1 H), b.4 (wd, I H>, 3,8 (a, 5 11), 1.4 gv 4 f B i be procederé is in «ccordanc#with ihe pmeeskdíp^pm'aúon 1*\ repiaeing the 4-brorao~· imsblyM H'pymmh «sed in Sitp B % B"i>p5ppbgp«MlMfe ll^MR (400 Mi lar.» droso^b) l^f^® % 1 HK 73*1 1 ΙΟ. 7.2/7J (2dd, 2 Hk 7.15 «t, 1 Bi 7.05 R 2 0.S (0, 2 Hk 0.^5 ix Oía 0.7(^ 6 i)
Trenara dan 4222 4-|4“|/<ír/"Batvlítlifntíthyl)siiyÍ|6x>,nniiia«]íl»i«|>liene“2”€arbffnUi,ile Flre prneokne acema s-x» n«h día puu »0 fhoparatron 1", tepíaemg $0¾ 4-hun>v-*-nHh\T /7 í\í i, >. u\»o r^^Bh * aio rot'aophe' <. 1 11 'vi, t SB N-VIR (400 MU/. Omso-dP 6 ppm: 835 (s, 1 M). 739 (wd¡ i 11 j, 7*0 R iwd, 1 H), 0.05 (0, 2 11), 0.75 01 2 i i i, 0.04 (s, V H>s 0. f 6 (s, 6 H)
The amines NIIRjU* xvhereh Rj and Rs5, each mdependendy ofihe other» represen! an aryl os hepavarvl gronp are ohtamed í« acenalanee vdth pmeesaes desenhed m i he HiemnuY (Surry 2014» 2, 27'50, (/baríes M J'lváM?Oigame Isófem, 2005, % 3SOS-30®§k Tteieuclton ;p#pii^i:$8í ;|iydmx>^'ñ?^4i0pv.pf the 4-&amp;«|lmophe¡io! deserihed in Preparador 2" casa be apphed in vamm secomíary «mines Ni 117¾¾ {a$ defined heremhefore) havíng one or more hydroxy funciions» when they are av&amp;iíablé u«umuuaih Vbemakseh. the -cerníais aiomo hmnig ai k s·^ m\r hydmxx aab f u-esn mnv he syníhcsised direetly in a pmteeted torro, Le. stariing írom reagems abose hydroxy tunctkm has been proteged beforehanri. Among ihe proieeiing grdpps, íew-bnt'vl(dnnoth>!Va\ nv aiai Kmnio\\ am Ai'cetab) puneneb
Among ihe amina/ NTHT.R.; huslng a hydroxy sobsdtuení iba! are «sed ior symhesísmg Ore emnp'Uu>da nf dio bu andan diere ma> *v meouuoed 4~i4.¡.,d«sdím>}pb*moL 4« 4 dbormi«bmo!phenok 4--;.Mlti0u;-4-nmdn hmbinoipheind, 444Hiri0iannnieibesy)aniÍi«ej' phcnot* 4..|4..pydm>iydníb«d]pbenní, ·|#ίΟ·Ν!«:^Ι4:#:^ο1(Τν ^ }a^^|pe«yi mctimnnl 4-(23'díhydrm 1 l74«dok6-ylammn}píiennh 4-((ld;p§lbyi~23Ah^^ P-> nammojphenoi 4-|(l -sneths1-1/hhidoTo->dUmii«4phennl 44(1-medn!- i//•mdol-b-\ vtwmo]*>olol*.v*ivi. 4 jil oiüMb\23,4AHiahuho-tm|dmohn3iam«iojpkmeL 4-p4- 4-44-(dieíhyl&amp;n'díKJ)adUnof flmn^L 4 v{23-4ihydíO“ í 4 ~ | (i ~m«lhyt~ I ¿Mndazoi -5-y 1 jamirio |» phesiol, «Ι-ΚΙ^ηι^ηΙινΙ-Γ^^ΐΗν^ΓΟδρίΓοΙ'ί^^ΙορΓορ^οβ^Ι^^οοίοίΙ*?'^!^^»^^?!»^, 4-|'í 1 ^^«trirn^lhyi^MihydrO' 1 //-indol-S-yOáintPoJ^betKs!» 4'-|'4>m«tboxy"3-{tHfiu6tiO“ sssetby Haíti i i*-o jphe«oi, 4-4 4~s mol h> ÍMdpímtnl) · .1 ·-^ritluoronioiby í>aníUno fpheooi. 2 - fiuoro4~|(!-methy!~Uldndol-5'-y|^5^lno!phep0l? 4*®Μ%Μ/Ν«ίΙο1··5^Ι)θ«ίί»ο|ρΙι«^, Ή* l*í.,thyi"2,3-<.iihy<lni-l//-iad.o!-'5-yi}8misiO]plie«<.>I, --4"|{ 1 '-ísopropyl“2í3“<iihy<|jro-ϊ //- :Íttáoí''5~>1)aml.»e]phem>l. 44femylammo}ph*mol> 3-|í 1 >meih?3* t l/<*!Kk>Í-5»y 1 )amíno I -propago!, 4--f(Prneihyl·1 //~h*üol~5-yUstíínio j~ 1 -bui&amp;saaL 4404ksoro-4-mef1plphenvih a¡oím>]pheíioL 4-j (>ohk>ro-4 -mbhyiphenynamino fphenoh 4-{{44luorí^henyí)atninüj-phenol, 4-|{! -ro&amp;thyf~ 1 //-pym>!o|4,3-b )pyr s4i? v S-yl )amíno]phenQÍ« 4--| (4 -tluonjphenyt}-umisiolphenoL 4-[(fluoiopheííyl}am?nojphenoi, 4>|(3>fluar»phmyí)amíno){áK»io^ 4-¡(2,4híí 11 uoíophenyI)amíno]phem>!? 4-|(3,4*<íiÜuoropheiíyi)amiaojpheno!> 3-f(4”hydr<.my» phesn S\aaíoo]k‘n<?oouíík\ 440'rex'íhox\phemttomm*s]phcnc*L 4 p3,h ilí|]uo*ophu«yliv. aí'seselplaaOh 4 's4-meslnIp Iva ^bammo]pVooS> 4-f{4“hydrowphesiyl}asiissH>lh®s|yp--müilsx 4-j* Λ-chjoropbu-Si}'!Ms¡síno!phem>L 4*{p)riraidIu3~yhmbfto)phem>L 4-j (oval· dxuy!-meíhyDasvsinojphenoK 3 H- b\dio\vphsan I ussshm-heo/omüsh 4~ f [(1 -methylΊΑ p>T;s/!si-4-yÍ)meíhyí]aí«ÍT\o^phcnol, 4-4;¡-7ciopmpyimcí.Íxy!mmi»;]phesiolí 4-{[(P-vneshy1-1 / 4 pura sa 4 ~ 3 ~y í} mes h y 1 b m n ¡o ) phe a o t 4-(hsa~2~yn-l~ykmmK>)phas¡:.4; 4-{pyrabn-.'í~yk amuKOphenot, 4-(£npdwO-yi«at>ioo''pbes.í»h d-íjníida/o* ' b.i^asoyhennh KpxuitMdn- 3-ylan»no)phenol, 4 - i pyridin-3~ylí«oino)phen<'L, 4-(i3x5~dín«on.v4-methoxypbenyh- a^ n!' el u Hp>*,ds' í »)p!x'ns ^ v " Γ* ose n^M Xvosesix miü o pivv, 2^phe«ylaminít}pyriroid(ft“5-oL 5-({4-hydr«>xvphesíy!)am tno j--knu:ti *>xybtsn*.opitrüe,. 4* ka-^hs ‘ .u^>!!'osh\')p's »yí|»&amp;*jiiíX>ipbi'Ri4 «ti >1 4 (soetl··* Laio-hOpK'ívi The h>irox\ tura nonse) oítho nxumdsn aniinoá k-oed alune ss Luí } px-uacd huorehand by a áüiiable prouxüng grossp piior to any coupiing so an acu) denvaüve oí sha compound. s>í Vnnuh Λ ll· as defased 'a Use yueedhg peocrb psouní-
Pret)»r%ti8n i; íerMlntyl |2»<3»i«áO'4'>(({3#h3»í»í:í^yÍ-3>4'>clihyilr«boq»*iioiüi*Sülí)» y! ¡ ¢:¾ rbotiy 11 pheny! ) vifsv 1j eaíT>a?« 1¾
MmA: 4^2^(ierí~§tí^^a^dm^ddímí}víh^ÍMdBmk 0$kt- ¿ma-Bnnl dhunTxmate tla>4g; 7.5 msmd) b dbsoivecl in M-dioxane p mi) and tl*c soSnttou ts added 10 a odConned sobaron os 4- *2 .entinaos! t>T)huvotc trox! ti O g; v« muro!} ín 1M aqueous sodiuni hydroxide $«!lai p!ML) aM IgCdtoxane (IOMS.% ándi (he mixture is ihe» stíiSÉd Μ :% b&amp;m&amp; The i ,4~á¡mmwíú tmmtM Éf Ék -vmmi :.aná liten ethvi acetóte |2|íTrnt) is added lo ihe residuo. The pTI oí ;fe :SK|OdWs fitisels ts|j»§|cd to 1.5 wfth 2|l agoepus HCI soluómr. T he óigante phaso ts xeptnM ófí' ííüd iteppmms phase:i¡&amp;-ifei» tóptmied xvíth ethyl acetóte UOOniU. fhe eundnned utoank' o^ueeis are xxexhíM unU xxuer, irhd u\er Ν<ίοΉ q ítnd capotea..! m ^ vulume oí 10 mi.. Ileptane i? added to lde rextdue and tirenThe solid precipítate is til te red oís" ío vieid tire tule cosrrpourüí. *11 NMR {$m MU/, dmso-lp .H*0h): 12 61 |ix n Ul OHm " 6' rao 2R 4,4 and <4Ήκ '/.31 {in> 21¾ »4 and 5Ή}, 491 and 6.51 (2x br a, 11), NITh 3.16 (m, 2R OH?)* 2.75 ó. 2ΙΤ C1IR TJSand 1.31 (2 x br st 9S-L Bu‘); n€ Mil |Í2f Mfl% d»·* mi Kit 167.6 ni),. 156 0 <í1k t45 ) <ax 1 ».6 *2 x t Tl\ 129 5 (2 xCH). ! 29 t tqb \S 0 Igl. 41,5 <01iR R4 (CU- 26,7 <01S >
Step Jb 4-i^~fieri'Btí0XYí'arhimí>ÍMmmiiiefh^¿Bmi^heuzmc avié I he miuiponnd oí Ote above Step {OJTH g, 3,3! mmolh iodobenmie dlaeetate (1.0? g; 3,3! >ϊ£«$|, kfrahut} lamtnunium iodkk (1.22 g; 3,31 π*ρρί)> iodine =(|ΜΗΝΚ$ J4· y$indl|. and d i acetox y pal ladr unr (0.04 g; 0.165 mmol) are. dissolved in i .:2~diehkrsroeíhane (S mi,), and tire mixture -s riten he,aed ín a mtcrovxtne reactor at 80'V lo? 90 mino tes. Ihe toolcd místete ss> g utnmned 6et\sevu s muned x'uoe s xa^ O, no ηηο> 110 < í t =¾ J t e nnema anhein. ihe organk nhase is sopamted oí), and t he aq neo os residuo is ex t meted wuh dic-dnd ether (2 x 15 mL). The nli oí tlie resehingaaífusous so Union is adjnsted m 2 with 2M aqueous HCI soíudon and the produel Is dren extraeted with erhyl acetóte- (2 x 75 mi,}. The urgente pirase is drled o ver NmSO.í turd evaporaled. The crudo produet is purdied by ehronratograpthy óver liióa gol m ykld tiro mixUaro oí regtoisomers, wSdoh are sepangod K\ v'U'|\>raio0 11*9 i mírg ^.aonJFA ¿"'vi .a'emnjn'de aa eIoa#&amp; Iho pH vi ihe upi'fnprñiíe cnmbúvd Aík den'- A adjnsted m 5 vúth NaiUW >md uven the neelenñrím is evafibrated «¡íf mnlir h rnmm&amp;m$ fey mé-^m dried ¡.o yield ¡he tulle compound. 5II NMR <500 Mlir, drn^-dm 3«0k): Π1η,ρ3^Η.Κ iiUO(41>. '8HS *^A> V|1V 1M iíl ! H, Ar-d’H), 7.29 (d, !H, Ar-SH), 6.89 (U IH, ^11),3.14(1, IR CR), 2.69 (i, IR CHA i -35 (s, 911. Boc)
Skm C: Β^4ύ4^^(Μ}^^.ίΗν^Μ^(ί^ί^ίί^ιη4(^(Η'(Ι~ eerfampijpheítyfyefkyífcarhgmMM·. I h.> eomponuil oí fbe . »me Skp (4 ΓΜ g 1 82 mmo ¡ HUI p¡ ?* g. 2 28 mmoi) \ t4hyl-#-ieoprt»pyl-pro|)att“2*etajne 10.94 mL; 3,4b nmmb and- íV,Aí-din\e.d)yspyrkhn-4·· amina i22 my) are stírred hs dkdik>fo.rmahane ;20nü ¡ ms 5 mineaw and then s2RtA-methyi \ \ \4~tmradundas \>ime ¡ ! >¡ *„ mmoh \<* Piepammm I ¡ h added, fhe mixture i* stirred íbr ihree;; %§ri itrmrs. The reactiofí mixture is dtíuted with diehínromethane í.ISümU and then washed whh water (25 mi A dned nwr NajSO* and cvapbrated, lile crude pmdneí is pmifted hy chromaiograplty over si tica ge! usíng ίο yield ihe títle compound.
Hjgk-ntmhiíkw mms speemwidry {t'Sí^}: l'motrica! munida. C^li.oiNAh |MRij* ealeakned; 52U 303 [M-bíf measured; 52!. i 282: 4Rt v; i --Ü 2911 cm 5. '•C O i?<K 1627 cm !; nmtde 1508 cm \ Γ-Ο-Γ IMS. H66 cm'5 ieanaraflid II; &amp;?#í-Rutyl (d-hnlmd-1f(3J?}-3«methykd^dihvdrm&amp;aiitimo lin-ll í B}~ % I) carbón > 1)Ikw.y 1 KsrhamaR·
Stm ,·€? 2~-f<mle-$~methvl-heiiZofc add· wwww<IÍ««<««. ... .v : ·:·αγΑ “.·.. 3-Methyibenzose aecd ibJ3 g- dSmsaKsh, iedobeamiie diaeetate (14,49 g; 45 nwmik telrabutylammomum, Mide Ü6.62g, 45 mmel)¥ todíne (i.143 g; 45 mmol) and (liycetswxpaiUídíuní (0,5 g, 2.? mmol) are dÍAsuk\'d so i .^diehloíoethane, and then ihe mixtun? is heaied sn. a sealed mbeat bS°€ íhr 1 boesr and 45 minutes. The cooled sol ut ion is purifsed by chromatograpícy over sil sea gol using dsehkxrósneibaste and enefhascol as duassis lo yiekl Che filie eonsposmd.
Siep B: Met&amp;yi 2~>imío~S~0mhyí^ben^mfe
Ihe émupmmd ni che abose ktep IT ^ k- 5T0 mmols ¡s dsvtobed m anddrons mediano! (i00 mL}« and Oteo SOCb (3,d ?tCr 4f idmob is added dropwisg -W thÉ o Talón fhe solícitos* is tolhssed ior ÍH honts and ibón evapotated to &amp; volunte cd 55 mi. and tkca poureá inCi dresshed ice (10i g|. Idie Msidag aiMure.....is eairaded xvtih dteíbyi eihec (d x í5 tsdd Itu· engásese picase is \va\ked vdth saturated agüenos KaNO>> sohstion and then brsne,. dried over Na^SQ* artd evaporaba! to dryness to yieid the tille cotnpoui!&amp;
The dém|ioad o? the abose Stop o,} pe 2u.7 tumoih l-bromo-ssscesnmsíde (ThTgs 20.7 mmol} and debenzoy! petmlde <0.24 g; I mmob are dlMolved in carbón tetrachlorlie ¡40 col > ,nvt * he o >eitu\ed ior bhosess. NVv¡ porta >ns oí Tsd'sx sosos* M o únale (1,0 g;. \t> mmal) and dibestaoyl peroxkie *T05 g; rtj mmoi) are ches* added and heobng ís continuad ior 4 more honra. The reacción mixture is cooled lo ambieat teenperaiore. The. iscsoksbfe parta are removed by filtración, and ihe cosseentrated líbrate is* then puriíied by Jtsscn*acs*acaphs Ove* sdeea gol nscctg hopeaste .md ethxl acótate as eksacsís to > a id the cctíe compon? id. W$B Μι ΜΛΜ 5~(amm&amp;metHi2h2~mdú~henmttte 1Ée éosnpotmd of the abave Sitcp (0.55 g; 1.5 mmol) Is disssdved in ?M rndhanohe ammonta. (20 snt) and then stscred at ambient temperatura· ior 1 hour. lite .solaban It evaporated to arynoss so yteld the tule emicpoesnd. vdbeh waa sssed les tbe nexl Μρ vbkiom purificados*.
Meüryl 5~{«mmornefey1)~240d0-befl&amp;«sate hydmbromkie (0.56 g: 1,5 mnu>í) was dssaolved te pvridtee (10 mL). then dfevn?~bu(y1 dicarbonate (0.80 g; 3,6 minoí) is added and fes n 6 Mar y m ferrad ai umhtent tempcratuse íor fe mnnnes and dura e% apos asen so drynese Πκ rttodne s\ refesaolved la soeteano! slOoil 3» and tees* 2\t aqueous bteOI! ateuoon (¾ mi.) and 2 mi ni vuner ara addad. I'he resuHteg sutetsosi 6; ferrad Ibf 3 linun at 50*V, shen dduiod nnh veasu' (26 mil. and Irse mefeanfe la mapnrnted niV andar redueed mesura. Fhe pll oí (he sesntenp aqucons sed felón <<; femMod ιο 1 osfe 2M «púneos HU sokssfen; Ose pnfenct. ss Oven extracten vvith eihyl aceites. The organic pítase te washed wsfe Erke, and i)a® d#M Ovar Na¿SO.¡ and evapmated tu dsyness. The erudi potetei k trituraied Idtl: DCM. libe solid tbfmedrte rMovensd % ñfefeion fe y tefe: Ihe inte composmd. SH NMH (500 MJHk, dms»-d6, Λ80 K): 13.24 (broad s, Ufe COOH), 7.9! (fe 1H. Artel!}, tefe id, Ufe Arfeüs. ?. s? Π, m, NI0* 7,00 (d, 111, (Arte'Hb 4.00 (d, tefe CIfefe 1.38 (.v. 011, Boc); ,3C NMR (125 Mlfe, ákdteb, 3§«:}: 168.6 (q), i 56.3 Cqfe 141.1 feg 140.0 (OI). 137.1 (q\ 131,6((Ή), idOJ (CH}?02.2(i)), 78.5 (q), 28,7((¾)
Aten Fi tert~Bmví f4-ío&amp;*~Ji~ífbWhJ~m?ikyl~j>4^Íihyiiwi$tiwmtt*ÍM”¿tiW~$'ífcerbtwyil· bettzyfjmrbamttie
Hu: eosrspoisnJ ofthe abme Stop (1 o2 g; 4.3 mmol*, TBTü (2.?#:g; |49 snpinlldP-nSyte .V*te*spropy i “propan«2-»sntee (648 mi.,; 8,50 rimad? and A, Ateisnfeby IpyridinM-amine teOosy? ase stsned te DCM (50.mí..) fot 5 internes; (3RV.3oinkp4'lt2,3,4"; tenaten.e^mjoteoLv o) s> g ' fe ruóte) us fe »« en Γ) t\ then addad má fea íplgkre te ferred tor 15 internes more. The ímohible pana are fefebOai tíy· fillkidp^ and iban iba aoncentráted líbrate te pursikd h> chmromography Over slfcn pd u$nfe dseblorumetbane and metbanoi as i.teams m >ield fes. sitie enmpnund. 1 (34?rnnns^4B!(3tl-3~anelbyP3,4feíby<lmtenpnnm1lin2pi7}~ yl }e»rfo«»j 1« he»^y!}carbamare feyp . lr Methri4~{Mmttmmtfkyi}~2~h?8mi}-'bem<wííi ámohaion οΙ'ρκΌϊν) .^buurso.ddbrmssorixahyidvinmme (i 5"? μ; 14,Ü4 nrnsoU m 5u mi, oí utethanol is added iiropwise to a vvelkvttrred soluhon of ammonia in sncthancl <?M; 'l'SmÜ rst mupnmt sempesaturo. ,m s dre soststs.m i\ tkss Msm-d sos 4 {«sao Vi she solvents aro evaporaren oíí ;md th*.- rrekloc ís pnrüted by doajmatoguiphy .«ver sil sea ge] mmg dseldotoosefsusse .md sssdksssm ,x eiumsts h' ^soid the litio eompnund. áStóEJi - - /i? o*f'buioxi·i’uΦ>>nyimmnomu'ihi'ifbeazoic mid fhi eosnponstd of the ahove Step (4 44 g, )o. o ossnob sv dfv-sokrd ni sorkiisse < 45 sos i and sisen vb-i'í f\-d»ayi dse.uhonsne (5 4' e, 54 o miools o; added and use mixturo w stsnod ai ambhmt temperan»? ior ib honra; it ís shen evapórate*! to dryness. The residuo ís lodtssohed m srsethmxd ( It!u mi.), and theu 1M .iqooooa \.n d! m.Iuííou ^54 sos »ts added. Ilu svnaitssig solo, m as n .kv ím 4 horas i* a¡'0 as i. o non adovwd r<' <.» ni o< otsdnent itmpuratsnx Oso ps! oí risa \oispsoa* .uhuated m 7 Sising 5M .uosemss Hr I soiuüon; toe mediano! Ls ihen evapórale*! oíí asidor redueed presxura. Tiro lesuithtg aqueous ndsman sv dümed wsíh water (50 mL)„ and therr the pü is adjusted ro 5 nsing 2M aqueous I ICi soiution. The pnxiuct iyeyts'aeted with diehioromethsnte (2 x iOOmtd. Tito nrg.ndc pinoso in vutshed sobo briutx ího» dris'd oves Nu\Sth ami evaporaba! so dsa sun-.N to yioki the titie coso poned widch is med so the rsext Stop withosH purifí catión. Íl: v: N~H; 3359 ot1; C-H: 29Ü3 an ;; ><>«; I68S, !603 cm l; anúde; 15U> crn*; €~C>~ O 1 M.H, iU*5. !*>57 o?si ' em^omijhemytk'm^amafe Πιο eompnsrstd of the uhwe Stop ¢3.7 g; U.d msnol). TBiU (7.19 g; 55.4 msoulh V-etini-Aídsoprof>yi'pn.span''5-amíno OJfsmf.; 22.4 mmol) and íV^V-dimethylpyridín^ín-nine |48 mg) are atined ín diohiororuothano (100 mi.) for 5 minutes, and then ORkkmetbyU 1,2,3,4-tetrahydfniíKKlum^ine Π.9Η g. 13.44 mmoi) (ef. íheparatíon Γ) is added. aml the ior I hour more. The iosoiahie pana are removed hy llftratron, ami theu the oppepplruted tiiiratc is purihed hy ehrosxasography o ver siüca gel using. dados mlsM <m *ís v> ( h sa·. s a d dw rifi». ^ as( eua 1
High“re$»hsí*&amp;R masa &amp;pest«»*»®fry (ülSHp 1 mpukvJ lormnO i <dB BBdΌ; jMHlf calüülaied: 459.1285 {M-HNf measnred; 459.1282 SH NMR (5(41 MIO Omso-dO, 3 COK., paaxenee ot amide roíame]: 7,66-6,8-- no. 711, ,mvn<me> -> M nod 4 1 2 nl, 20, CH.-n-nqumohneh 5 O and 4 *0* and * 8 ! and ' 87 un 10, CBvásoqninolme), 4.17 (d, 20, ClOhermi), a. 18-3,48 (m, 1H, CHrisnqumoíme), 1,16 and 1.14 and 1.11) and 0.98 íd, 3H. Isoqumoirne Ohk í ,31 and í Al. (s, OH, Boc).
Mmmnte L ST5-Odnr«-2~f|0#}~3-ni«íiiyBS,4-dibydrnfe«í|«1nnlm-SC11/o p!Jpnrlmp|dlpta8f!|*7Ndd8fd fljhl lig j; Míhy7 $~{S~eMí}m~2~ll0M}~3~meÍh¥f'At4+AÍhyémÍmqmmÍm~2(lH}~)>Íjearlmmij~ ph envfy ~ í s 2^¢íimetkβ~H:l·p)rmie~3~€^ϋ.\yhm:: a no n?n < d' k a K ¡.nnpsnmd ola -a kd su Id en, a anón ! <405 *nmuO m 50 nd oí dsvhjoromeduuve lhe?e are suoen^ivcls added 0.7 a o! íhe eomfxmnd obüñned in Prep&amp;ration V {4,79 mmoO, 0.7 g οΠΙΟΒΤ (5.22 mmof}, 0.81 g oi'BDC {5,22 nunollMii i.6 mi oí' me'.KOmine v7i 7 nonel- loa baieb 0 thtm sUrre-J overnlght at atnldem ieMparabire, The reaciom mixture is ihen dituted wiíh dichlmnmeihane and xvashed. 3 al mes vuth raumued aqoeooe NaMH\< rolaran Ί he oréame pnase is iban dned mer MgSO,$, rdiered, eoneentrated lo drvoes.y and i lien puríiied hy chroimUugraphy «ver Olea gal uylng dleldorometliane and melhamd as eluants. *0 2*310; 6 nOO MHT'. dmsn-dO; 300&amp;}; 7.6-7.3 ím, 3H> aromado Os, d-dborophtmySl; " 2-6 8S $m, 40, ¡í v 0¾ uritvrndioi mqjmelmed 6 O f> - un, 01, II pvjoleo 8 o. 4.8-3.8 (m. 01, aliphatie 0, looanydroísoouínoUnol: $.3--3.75 (dd, 20, aüffeade lis leuahsduíí-'oquinolñud, 40-1,0 un, 2!0 l íCH.-CHu: 3 2' ($, 30, C’HnN-pxrrok), 2.0-2 2 un, TI, alíphalm Ha, ietmbvdíoesOqumol mer N <v O s, B5-p>,, oí q 1 ^ U ¡O, OO OO 0)'· B05 id. 30, Orad yd roi soq uínoime-O-h i IR: vi >0-0: 1693 cm's esier; v: >C:::0; 0125 em's anude
pkBmy^Í^iÍmetkyí-íÉ^m^^»€iiírimx0mmM
Treasolotlon of L? g oíthe compmmd ohiamed ío Stop A «3.7? mmol) ir 5 mi oí dioxanc theng ís added 0.3 i? g ofiiOH (7.5 nnnoH dissofved m S mi of water. 'The haicU ix heated m a mietowavo apparutus for 4 ho«*$ ai ]0O°í' aponer 140 W). fie learUon mixture *« ífeen ídfered and concéntrales; ?r> dryoess. The reaklue íhenefey obtained La laica up m dichlorbisiÉianami) and ibiliaiamicd arpíenos NI HCl solnílnias addM íteaioi The «rgrmkr pirase- i a oa.vhed vuth nater and thas nidt Pune. drkd ocer MgSO$, filíete·.! and ermeemrated to dryness. I he tüie produel ra obnbned i» Üte form of a so lid and is «sed di rea!; y in the nex! biep. %::tejSí.8:(5fMí;^^:;iÍaiso*d6; 30OK): 1Í.0S (broad ss f|i CQOHX 7,5-7.2 (m, 31 i aromado Ha. 4-v44omphrunB. 7 ?~n 9 un. 411, aroma! ;e Ha, ten ah t d a«soga; aoh ne i o,4> 0.3 (m, UL aíiphaüc H, U pundo); 5 i3 7> g!d. 31!, uliphade Hs. searahydroísoquinolinei 5.0-4.8-3.Η (no IH. aliphaüc B. íeirahydKdsoquínolsne); 3.5-3.2 is, 311, Ol^H-pymde}; Mi 3.1 (a'dphatn. Hs, €U.vionas\.\|nmoime); 2 5-2,4-1 B8 (m, 31b Ol,rpyrrolep LO**-0.S2 im. 3H. aliphmic Hs. CHb'tetraisoquinolme) IR ' v' -OH' ^0n~30¡L) om's earboxyLe .;u,o -· C-li ,004 » lo 58 cm ' eatbmyhe ae;d; v; '4' \-· lo.?5 > m ’ amibo
StepO N-'(4'í¡íen-'Muírí{dhnefhvÍ}sfhiiox¥Í¿>henvÍ)~5~($-chk>m-2~^Í3R}~S~nmhvi~3J~ Μ^ν0ΒΜϊΜ$Μηΐ^ηΐ'2!ΐΗί~},φύΦοη}ίί{ρΗΒ}!ΐ'ίρ}^~ίΗϊη*:!ΗϊΙΛ···{Ι·>·ίηί'ϊΗβ~!Ηψ}Ί\ί~»>ϊ~>$-ii}'W»prm>k"3-<. arbaxamtdi* Ίo a Nohitmo of pop y of the eompauod,·oHarnedem.Stop B 11,5a mmoh m l* mi of dlchloroelhane iherc is adácd. dropwise, 0;244 m!, Of ;!i-ehhn<v.V. AiddnnraihyBprofndmno I-omine i he maetmn ‘msour ta súrrc>! ai añádete tempuenuu· fot I lana', and íhciO a?e then ihLkd uno g ,o' me ·.>· tupo uso! m heperatíon 1" ti 84 nnnol), 10 mi oí Jiehieorileav .m.i «i mn g ot 4 .Umeüu wn'nsof'íudine (DVAF: . *{' mmofl i ss. haieh k Meced at 'OH* merman Mu.* uanae-r mixture H ssaitoace diwmed m diddnromei une and. mndud vunt mmro'.cd mmc<ma NidfCO -¡Mimen, 1 Le mi, pm.ioeMx obiaincil ñs ihe fonn oían s-d and is o>e;j direeíly m thewst Stop.
AfeoMi] $"0*€k¿ámy2 $0 $ím®ím> mrhkmmkie lo a sobo ion oí 0.5 g οϊ Ihe eompouml obnaoed m Slcp 1' tl 05 tnusoO ns ? nd oí nieth&amp;nol íbero are ndded 1.-55 mL oí a JM sosmion oí potassium iiydroxide in niethanol (L55 moioí). fot- boit.lt s\ asmed as amblan! icw potáoste bu bt mmoo.'$ íhe coséis no mixture is then dlkned sviüs dichloi'omeshane and suceesstvdy wasked wiíb Hvi sqí-teoos 1K1 *olutinn, ixmtrated tujoenus NalK üt sokmon asid then bnne umü ^cutral. 'íbe omame phase i\ M í dssed oo M$M>, P naed <a d ^aposated Ihs. cría o p oduvt *on n ohnined 1¾ puníled by ehromaíopraphy os oí siliea gol usmg dieidmorneíhane and nnímnnd as olu.'uta Vbo solid tVrehy ebkdned sx dissolved in o nnxlose m osuer jsOíotouilt* ussss- kssoíuünst is tompkm . Πbeted and then hophikmi ffemsntei microtffiúfysh; {%> ikmnakai:mpín:areá) %C::bd.7d:68-2S; %} 1-5.43:5.69; %N-1 1.79:1 1.66; %€R\9?;$.95 iligh-resahiíkm mas.« \pmirmma'ry (ESI*'};
Empírica! formula: Ck^híClbMk |M+H|' caículated: 5S>4.2366 \ Mi 11 }* measured; 594.2289
Sd2"(|{dS)-3"(AffilM«mefl?iyl)-3iÍ4*dlliyílro^Oíiol»olli^2(ll/)* >d|«arfeoiiyl}pli«siyl)*1-»^rlíyÍ-i%V^1p^«íi>1*l^pyrrokv3<8rhox8i»M#
Men A: íerEButyl $$38}~2~β~{4-(4ίρΗβη?&amp;^$&amp;Μ0}φ”1~Μ#ί$φ·}Μψ$*Μί~2~?ΙΪ&amp;«»®(φ#* li2J,4~knmkpdrúkmpam.4m-3~vÍ^ni^yÍiearhmHüh>
The tute cornponnd is obtained in aceordanec vvith Ote procesa oí Exampíe .1 usirtg íhe acid oí Propasaron 2 and .fncobs-dvi .2kk4'atnrah}nimiso*gsintdin-4 ~v!ntethy!]earbaioaíe ptee Preparatkm 20 m ütep Λ. and Λ'-'pdcnyksiiimo in Stop C, ΜΜ^$ί*Μ!\~$ρΗ*η?ί~1Πψ}>ηοί#~3~€β?&amp;&amp;χ#ηίΜ#: 4 sdlodon oí (he NH-IW compouud oí Stop Λ in dichtoromtdhattt: is placed at 0°CS |&amp;ití^tórequivalen!* oílrdlooroacehe acal are added choretodropwise. The hateh b sdrred <tc asnhiem temperature ios ^ hene·· ssotií the sksrthsg nsasenai has dlsappeared oompiesdy. tbo i»uto¡j ¡n'\t''c λ en ooí x o Viitcv !> puposa, bes' up >seeo' us too oosako uvíce 'Vstts tolueno.. íhen utken up m a mixtura oí accióname'! SO and Anally ivophiHsed. i he ütíe píoduet b Ten o human! ahora ncuiralisaíion stop.
Efementai Mipmmtmfysm (%t thetm'ticakmemttred} "iiO-77.7S.77.27: %i b-e.v/rS./T ΤΛ-10.36:i0,44 :|4l>íCllero»2«0í3>S}~3~íí4"melhyl|>5pcra^Ín-l»y!}MeÍhfÍ|^pl^dlfeydp^ Éh|s|ftp||i^|l:lÍ«yí|earh^ny^pheMyl}-A^í4-hydcexyphe«yiH^|;!ÍI»i»Í!®ÍSS^í|«|«
MeÍÍyÍ*Íi^-pyrmlv4-prb<axaMide
Sim ele iemBuiyí meíh?í~ltíy}rmte~jiij€arh#nyÍ}a^fi&amp;0M¿iH<toie~Í^vr&amp;0X$i®8 !ha te le wopuxuk! - ni o red u. acv<cdkpoo ^olahe |aoce\a «u F\v.oofk I o^mg do aese* oí Preparación.: 3 and :ÍÍ^34C^®^byl-i^lpera7Í«yl:|i»#É^I'|-,:1,2>3,4''telTahydrO’í iso«|^»i0l:ik@::|seerfepaui5i>n 43 iu Stop Λ. and (he compoumí oí Prqwation 3’* in Step O.
Stei* ii' *'{$'Chíont J'^(JS¡'3'it4'fmiiiyipfpentzin-i')iinteíh}i¡^,4'^ihytÍmm^itiiti*im-‘ ?(!H.h)i¡e{fthjH ii¡phr^H'!''X~{4~h}rh'mvphemi)~S'~(Ííi~ímk^5~}4hl~ti}!i.>fkrÍ~!lÍ~ pyrr&amp;í&amp;'S-i’arhitxtímíde
To á sohdldn tvf 455 mg: (OJO Tm«d|) Oí the igrhdtsdi niiaíned In pgp J¿ &amp; f mi, of meihano! ihoro are added i 35 mg i 3.5 mmol) oí KÜH. After sdrríng íor3 hours at amhiesit tcmpcuturc, da» teaetmn m'\tuf«? o vonccnuaaxl. i«o.\?ed \u*h saturaCed aqneous NalICCh ,o luden and exlrneted noh moíhJen*. üslonde. (he oreaoic pisase b then dried o\er \n v) 3ko>od asm o\ oom sed to 0p. m ^ The on\' pmduet the>eh> aNamed s\ Oten puuhed h\ {.hasniutogíapio ove? -ulna gol uahsg iln Idorontethane and meihanof M etuahcs so s¡old d'o e\peded produnt m the íurrn oía mam. fa», thetveticuiíMaixuredi jet iae
High ~re.it f>ia ¿km mms specíromefry (ES f (); ilknpikodi foífouifo CbAíOdaCfo |Máf:lf eateukfod: 713 J0D7 1M- I ΓΓ measumd; ?!3.2973 liaiMlkix A?»(4-Hydr«^pfe#iiy!)*ÍV.(m-ind«Í-S*yl>‘l*MiiílhykS-C6*{l(3S)-3-í{íl· metl*^lpipera»:i«-I->íl}i«eílíyl|-3^*dihy<lr«ásieq«¡ri^l*ni*2CÍ#í*yí)carb»»yl|”!3v' beímsdkxsoi-Syvdkilí-pyerrded'awhoxumirle
The fofo sompound fo ofoafoed isr^eordasce- with the oí' Inampic 3 ustog foe pppppund oí Preparaüon 4 in Step A.
Ekmmksk mktmmaiysis: {%, fheon;fkaí;meúst*red} %(>7!.45:49.32; %11-5,86:5.22; %Nfo1.65:11.08 ÍSigh-rgsefiHÚm masx specirtfmeíry (ESl·'·}; kmpincal formula: (.ífofolfoCH fMAHf cafoufomd: 723.329S |Mfoi;: mensure*! 723,3262
Urdese ο formo se me«üo«ed> ílu eompoemds oí fot· tbllovrmg Kxampks are ayorhexbed m aeeordanee wifo i he procesa of 1:7¾ ampie 1 nsing, i» Stop A; A; che npproprfole odd oht&amp;med aeeordiog lo ooe oí Preparo Horre 1 lo 32 and (o) tire appropríale te i r a i>y ú ro.ls otj o i o olio e eooipnond tfolafoeai ueeording lo ooe oí Preparadora Γ ίαΨ and, in Siep (5; (di) fot; sufofofo Alt Rd-U a o fose (a mm~ exhaustiva U$i m proposed i a Prepa ra dores 1" lo 42% lite eonspimmís foereby oblo i ota! are optkmaiiy su hjecka.l lo a stop oí conversión mío ealt foro? lis Ib o prest neo oí HO !n efoer, Afíer filtrar km and lyophilisafion in a mixture oí acefonltrilefoafoíy iba hydroehforkie oí fot espertad eootpoond b ofoafoefo dihydi^ísuí^ukmllu^líl^ilcarbanj'i) ph£8yi)«A9C4"b;ydru&amp;yphen¥l}- i "meíhyl"iV~{I ~ Eiememni micrtMmuíys'is; (%f ikeüretícaí;measuredl} %0:::64.;54:64.9; %H;;o.67;5.49, %t#4£L§; 194; %G::;:D. 29:12.52; %Ch::;8.86:/..19 me!hyÍ~17í'4siili4'\9'jI)9J!/'pyrr/9e'3H'ar9o.s.a/!9/le
Higk-rewiiittifm mms specuemstity {ESi i·}.;
Ernpirical formu!a; C uI 1 .t$C I 619¾ f MtH] ' s sO col alad ; 241,3320 [IvHKf measured: 74U326
High-remiuiimt mms spectrometiy-{ESkt};
Empírica! formula? C'^H^ONíCh ί M i 11 f calculau>d; ?53.3 320 {94:ríll: measureíl; 753,3906 i»dttí~5-yÍ)“I'{|iru|>aií“2-vÍ]|-IÍi“pyrr<ílí:-3-e5*rhí>xaittl-íit
High-fesvhEkm mms specírometry (ESI 69 Empírica! formula; 139 f.<?C'IN<ftr |M Mlf calcúlate!; 255.3476 IM-MC]' measurcd; 3955.3460
JktllMlüL AJ»í 4" íl y ú m xy pfe i)-· i-m&amp;hyUN-l í -mothyM II~Ip $1¾ I-S-y t f || ,1? p 3-Í(4-meti^lpi|>«r5ul«“l I ll,l"V I ¡«a rhwyl} -I3- be»^dio«0l-5''yi)-l|f.pyrMk'''3''í<srl5«sís^i:tík> hydm?t*k*rkte hi&amp;mettt&amp;i mietííutmfy&amp;k; fke$rgffcai;meü$ífreti} %C-65.2<>:ír5.S: %l{-5.73'5,47: %N-KU8: 10,48; %0=8.76;8.46; %CÍ~-8.?6:8,G2 S^5-I?luom.'2~{|C35"h3~(m^rpkfdl«'4«yli»#lOyl)-v354-dilydrsdso«|«lii»IÚ5<· 2( i 0}-ySlcarh»»y|| phenyl>-iV-<4^>'drft^phe»yl)4^^ÍiiKt^|^plíis'Pil»Í:|^yipilé' 3-c»Hm>x»mide ky*f roslíl&amp;yidy micwemfymi (% Hteoretícuiimtímikre^ ‘HC>69.11.08.56; %H«=S.8.5.31; %N-8.0é;8, í 3; %Ci—5.Í :-4.68 tomate. ü« S-CS^luo^^ll'M^S-'Cmiuiíiiidm^-ytetlsyiVS^dlIiydrolsíííiuiaoIia-l(lll)-yÍ|e^rlK>iiy!|pb*a>4)'^H4“lmlrexy|>h«:s5yl)-!»{3''lsydr«s:ypnspyI)''2'‘melliyk¥<-pts«&amp;y I-1 íl-py rro\t-$--ta rh«.$amkk líydrocbioridé·
Skm '4.1· ΦΊίρΦοΐ$ύ$Μηνίίη»2ίΠΐ}ψί/€ί&amp;&amp;ύη$!βρΗί%ν^&amp;~{#~&amp;¥3^ΦΡ&amp;€!1*}>0~2~*ΐ**:&amp;β·-Μψ&amp;**ΐν&amp; it^pyrr&amp;h^cerbüx'&amp;mide
The ñtle troropouori h obtained lo accordancc \sith ihe procesa oí hxumple 1 usiag Ihs acM ::Pí'Pr^jpmííük10^.Ésd4fe'd%íií£>mid o i' Preparas; o» A iryStep Á. and : ísíp:' paraísos* #m i le p €. Étm 8> $»0~Μύύ?&amp;24Η48}~3~{»Μ?ι&amp;ύίί®^^Μ&amp;Η}'ί)·"3,4~4&amp;)ώη*ίΜΜϊώη0ΐΜ<·3:{!1ΗΙ)* χί?€α?&amp;θΜ0ϊι^&amp;?ϊ}Λ,<·ξ4^Η!η>\γρΗιί8φ~ί"&amp;'&amp;ίΦοχ?ρηφχί}"?-*Μί&amp;}'ί-\φίι*'ηνΙ''ίΗ~ j^rmk*3¿eiírhüx®mM&amp;f l'hc componnd oí Siso 4 s4 Hih^vted o :s dopcoteeilHi rea^Uon m ; >. aukmco ^iíh a ponoc.d aoaieyons :o that dtvcs 6,.9 o* Sup h oíí xanpdc 4 >
Efcmmtat mknmnúivsis: (%> theoretk-ahmemmed) %ζΡΜΜ:07Μ: %!r:::6:S.79; %N:v:7,58;?.6h %ίΊ··:~4,8:4 75
Migh-f'esohititm ma ns spectnmteiry (ESh };
Bmpírica! íormuUí; C $ >Rn PN *0-s | ΜHPj! cabuíated: 703.3 776 j^easumd; 703.33294 i«dssíjt*5«y!}»l!£“|>yrrate«3“Car!>a$amÉíl® isyc!r<K'hlorkk Eiemetuai miememziy&amp;is: 0’*. thnúreih'iií^meiisttred) benaod *o x of »5 ~y i}-1 H~ py rro I e~3 < a rkmi m ote Iiy árm:h ín π d e Eiemenüzí mkrommiy.iii; (%f thetfretk'ahifteesurefi}
Ex ampie 14, /V^-HydmxypheiiylH 12«cIhitel0y!“A,''(k?»et!yy!''! l#dMte.el~5~vt)~§~(fE hsn*o<li«x«i~$-yÍ)- 1If-pyrns led ~eedomomié« byílroeblodde
Eiemeníai mh'riuimifysh; {%, ihmrencu!mmmum0 %C::::66.63.no.54; %Π 5 59:4.67; Ό.Η ;0,73,H4iyv ”<..·< ;-........I 5?:-4 2.4
Ex&amp;nsmle 15, ¿V-i 4 -14 v 0 roa y §> k ^ n vil -1 ¿t-dimd ΝνΙ~Μ6- f f í35>Mím^pb«Na~4~
<wwwwwwww\,^s%s%ss»sssssss^ V .y v v Λ Λ v K \ y \ X 1 il'-py rrok-3 ^ea rbex a1 a i de hy ú rodilorkle
Elementa? t%, fheoteñéaíí^áMréé}: ^^Μύ?Μ%:?Μ^±7ΜΜι%Ν^77^^Ψ0^Μ0;,11
Migk^MÍMimt mms spéeímmmtyy fESlk}? ümpiriea! ro:mah¿: (As; ΙΙ^ΝΟΟί, |Μ:·ί'1·ΐΓ e&amp;!enla|sb: 685.3026 pM&amp;ypi'©d: bíS Jb33 E&amp;otiórdo té, Af^3~Fl»«r®-pM»yÍ}'iV-(4»hy^r«s:ypheny §)~iximetit^'I^S^i6-([05]h5~ Clii«rpfeoiiii-4-ylmtitbyÍ}“3í4vílíhydríílsííq«moliii»2íl^|*yi)€»rbímyl|-'l0-t>í?«*odifts;«l· 5">Ί}-1H" py rrn te-Ot-ca r feo x a so i fie hy d r oeh lo r í de
Ekmem'ai mkrúamtfyxh';' 69, tkem\Hk'úi:measumi} %t>$&amp;.62;66J i; > %N~?M:?M: 5tó^4J:SJ9 ICxaTO.de 1?, S“í5*Chloro-2-11(^)-3-(1^0^110110^4^1180^1)-3^4-(1111^(1^1804101110110^ 2(li!/)-yÍjearbttoyl|íiho»>-|}-A'"<4-liydroxyplie«yl)~Í>2-d*meífeyl-A«pfeeityi'‘ÍjSf-|iyrrole» J'Carfeíísaabdií Isydroehfesríde
Eiemeníik miepeimaíysh: (%, thgíi?8iiaii;mmmreti) 56C-97.S1 :6é 91: %j 95.66:5,05; %N-7.S7:7.HH;
Exornóle 15. H2-íiydroxyethyiHVH^“feydr«j£ypfee»yÍh2~methyEM&amp;«f{{3<S)*3-j$~ {aí«rpla:Oin0-yl)fctfeyI|'3.4-dsOydmOoí|«b'solro~2{l /7)yyl]earlHuwl}0v3~feeaíTOlioxfd~5~ y!>-íV~|iifee»yl-l/l~|jymil«“3"eorfeoxoi»lde hydroehloHde $t£i> A: Í~f2"(B£x%vloxy)etftyil~N~f4~(benQÍox}'}phiitiylj~2":m0$kjít~5~(6~{f0S}~3~j2~ (mmwll&amp;tm~4zVl}?f&amp;vlf-3t4~íÍikydrtmoqwn&amp;ím~¿{nihyt¡Varhmyl}~ilJ~bmZM¿ltíX{ií~S-~}Í)~ N-phenyl· i ÍEpyrroíe^airlwxamide f'he tille componnd ix obUined m neeosdance wdh íht* procos ol'Exanípte I using tíío ai. id «F Prepararon 9 and: {3¿^0^|lH^»^feolíM->d}elhy!¡”ix2J:^4«|rifej#d||fe<iUtol!;feó (xée Ptlpatlfidn 50 m Stop. Λ, and -l-thenzyíoxyp;V'phenyianilioe (seo Prepararon 8”) in Step (.'. i~{2~ίίytfmxyeih}Ί}-N~(4-hydraΐ}ψf*enyί}-2~meώyl·$-{6~¡¡{3$hS-β~{Mitψkoim^* 4~y$)gthyif3>4~{likvdmii&amp;qiim8fm-2($M)-}ijc(trbORyÍj~$.J~hmziídítKi;o$-$<-yí)--N~phemi~ 1 $í~pyrmfe~J~carho.%:mmde kydrm'kiarkie
The pompopmi af Bfyp A !i tel>|ee!ed to a deprotepiimi reaetto in lecordáaéé wifts é pmfpepl simipgphs |p thg| hessdbtá ln S|sp B o|v!Aa»5ple II, Hígk -remimkw mas? xpectmtmfry f£$H); ktnpiricai ibnmU: <\*í |ΜΒίΓ calenÍaícd: 72>U2U j Μ Π1\' measured: 73 5232
lux amale 1% A-{4“Hyílraxyphenylh5“(5“i«ethoxy»2“||{35)»3-<morph»Hn-*l»yÍmeífeylh 3gl^!ih¥dr©ís^MÍiwÍiíi-2{l//)';vll«^rÍK>«yi| 1 ^I-dimethyl-A-phe^yi-I py iy d rpelsterlcl^
Eiemekiai microsftúíyih'r {%, th(*<fretícai:memure{í} %C-67JAbfAS; %íA6.13:5 94; 53-7.92:7.76: 7335,91:4.58
Bsampk 20> A-·(441 ydtmyphmyíl' S~fS-meihayy ~2-f|(3A 93-methv 1-3,4» dthy flrak<M|uÍ80Ü«”2f í 7:i>y 1| ea r he ay 1| |>h<et* y I)-1 A~dd»eíliyBA7(pyrklm~4~ylA!IÁ pymtkK^arhaxamtdi: hytlrochiodtle EíemiWifií mh'nmfuilmh; {%< tíiearetivaiímemanuí) %V-C7.USh6v.6h; %H-5.66.5.46: "3=3,977,92; %C)~-5.69:5.29 ílh>h~resúhkiim nms.s ¿ppctmmiwy (ESi^j:
EmpidesT ibmmk: |ΜΑΙ| *' éáloislated: 38? A#3 ίM Al] measured: 537,2649
Elemental mlcrimtmlyste: {%, theareücatmemured) %N===i 137:1 \ 27: %0·-===43:4.71 lligh-nnoiuHim masa speemwiefry ÍESf r);
Bffipmcal ferí«u!á7Cki4:l4^N<>Gíi: ÍMGlf pafculatsscl: 7035236 ¡M%Hf fteasnMd 37633234 E&amp;asimfc 12. jV~<4»S5ydroxypfe^ssyI}-1
Elemental miemanainh; (%> tjteoretkeL’mem&amp;r&amp;i} %(> 68.61 74(:1-==4.82:4.27 m«6r¥l'3olH!i6ydr«iso<p6s)í44i->2(1l/)--y!|«ar1>««vil4,34>«iv»idio>:f43--yl.|''íV-'f:!'-ffieyiyI-· Step 4: (6~f¡(3Il}~J^meihii-3akd¿lmlrmwquim.iÍÍn-2ilHh34¡€0rbmn4^'líJ2mmHlkxik'S-3i)~N·-i l-mefhyl- 117 -/η τ íí;:o/·· 4 -17} - i / (~p ¡ >m4e - 3 -aa rbax a mide
The íiUv oomponnd ss obUíintU in ;u oorJaus:c wdh sha procesa ot'l'.xaníplc \ u4ng Ou· acid of Prepara! i cas 7 and ihe eosnpoimü oí PreparaUon 1!> in Sl«íp.£4 Το Λ Muüoo oí 6.86 g {8,Η> mmai) oí ihe eompound oi'Stqj A in 70 mi, oí aahydrous iiitfíapol there are added 07 g víféiC 1®4 hoiírpaElitei surred at amhient temperatura under dihydrogen pressure <L5 bar) íbr 12 hoias, 'fbe feactloa Msiwe A then gtlterdtaitd íhM áÉÉeeti^ied 'ted^aess.. Tbe pxpeeted pradaet ís i^OvmM ib íbe fbrm oía solkl JM NMR; O MX) MI!/; Orncn-M XMk) VM4 Mí! H pviu/ole*: 7.1-6.8 <s, lii 11 $$iÉbié:|¿:: '¥ΜΙ^:'φ^:: US) :»rpm#íf 'Hs, H teAahydMtiooamoiibxl; 7.0-6.7 (*n, 21T aromaba H$k 677-6.4 (m, 2H, aromado lis); 6.8-6.4 (m, 2B, aromado 1:1a); 6.1 ün, 211, y<.TIjOV> 5.2 x 3,0, i/? (Μ Η. H inmole (presence M coniurmadoíia! bomoiNh 5.! 5 pi, ni adpbibk' II, H icíraln OsosaopuiUí diñeX 4.0 {.m, IR, alipbade Hs í!
ietraaydsoisioí.pdmsdoo); 4.9-4.8 (m, IB. €11(317); 4,7 (m, IH. aüphatic Η. H ®trah\dí.oí^'4.paosSUs.wX * ** ul lid ahpKatb 11 H teirahydroteoqumulme'l; >.8> ρο, IR aüphadc Η, H teirahydroisckjuínolhk'); 3.7 (m* 2B. aliphabe Hs, CII¿CH?0H); 3,75 (2s. 311, v. 3M3.2 (m, 2H« aüphatic lis, ClbCHsOHl; 3.0-2.4 (m, 2B:. allpbilk R B teísahuboiaoomoolmcX 0,4-2,3 31 k €tljrf>n>>UR 1Λ 03¾. 0>7i (4 d¿ 31!, ΟΙ,*- TH1QX 0.85 (broad s, OH, Si(€H RfCIIRB MR O. ! 5 (op 61!, SirClMACHlAlHsIs} IR v o 4 I ' Bo ota * ar'oov. lie acal; v: 'X>0: i 621 cío ' A6é? €7 $*{2*Η$φφ$\ν&amp;χ&amp;^(3~Η\>όη&amp;γρ!ί*'#νΙί~2·-ίη&amp;&amp;χί-$-(6~Μβ&amp;)~3-ΐηΗΗ} i-SA- iUkxdr(^iHium<íÍm~2{i7'I}~}iÍr(¿rh0m4}~iJ4)m:j)éhxí^S^^y-(l-meíh}i'iH~fm<az^l·^-yfy--///-/>)X carboxemide
The pisca o i íuneilon of ¡he eompoítad oí Step B is deproleeíed foflowmg Ore procesa oí Stop D of Bxampie i. 0 <* 08 0 ('68 1 ,V' <4! / '' 20, " „N IMtX 10, ~9
Empírica! lala: iMHIf calcúlate*!: 634.2662 [M ? Bj" .meaxured; 634.2660
Exaaipte 24. A^<4~Hydr«xypfeeiiyl>»S~CS~i»etii«*^||{3S)-3-(m<w*|ili«1Í»4^i|^fÍ>·· pyr»*»!-4-yl)-1#l~pymde4Warfm"ísadde b>xf rochlmide flememet mk f\t kteorelk ¡íi-medinrek} %€™SSJ6:64.4?; %ík6,09:SΜ; SN»l1.02.11.45; %CÍ--4.00:6.7
Migk -rmüluikm ®« spaeímmeiry f£Sf*} í Empírica! formula: Ο^Ν^Οι |Μ-ΗΓ calculan·* 675.3289 [MBíf me^urod: 675.3207 llamóle 1S» I-p*€7|!6?m2- ||0^)~3^aKsrp6«lm~4~ylmí;ife¥l)-3,4^II6yá»isííQ«l5i«Ii»-^ rí»y d rI l*y 1~ i pyra2«E4-y lEl76'¡nfrrí>!«-3-'«8 rfeoxst ái tde hydrdillorilfe IÉ¡mkMM {%> tímúreke¡}í>mmmm'ed¡ %C;;:63,77:62.83; %ik5.63:T83; %N-I !,74;I L29; %CB^.95:5.42 ¿yT^lSfdí'asiiífecsiytlili^^^ mctbyl'‘3,4“áÍbytlrolseq«moli»«2<ÍfíFEyl|c»rfe<myI}-'t3''*Hí»*odíojtol-5-yl>'*A-(l*meih3fl· I fTf>yr**«M-ylH H~pyrriiU~S-K*rh*mmute hydraefclarhlc
The procedure í\ m aecorüanee wilh Síeps A, δ and C oí the prutesa of Exampie 3ü xpl le'n* dx> \ kUsmí 5 Uf i v i ü S\\> Π n nod \í,mo s \ \*r ouu kuhom >^o i í<x gei (CB.T.lEMv-OM/Nlh; gsadiem). the eompouuu obuomxh 0.3 g (ü,46mmoB. B dLs;«.dved m y-mL o&amp;an ,\dn us ί» *nn>> *\ fkxC i< .Akx! duvnx^e d *n> I· m *0 4 * *' > <. * ,>i 1M Bi s .noIuooo m efher. Ote kueh x xúmx! íor 0 5 hours af ambienf temperutua·. hhe pseetpíkno tfvíebj obíamed a Ekicd ood dned .md the.n dissoíved in a mixmre oí.' SMsClN/liííC) ilstlng" tyophHiypd ti lpw; ieinpemi^im- Ttw -&amp;Μ|^6ίΙ prcxMat ls
Pfiiátpod lo : Pm tpsfrs of t solM. mk'nmnafysis: {%, íheor^dcaiímeúHured) %0^>5.{S;S4,64; Wl-lilSiS.PS; %H2.542.5; %0--5.19:5.39 flígh-rf>Mhittwi mess $peemw¡etn·· pV67i/;
ImpMesl lomuda: CM^Np'K |M;-l!f calcujated: 647.2901 (Mi-1 i p uHauaued: 647 2922 faampk 22' i-¡2-(Oimieíhylü«*iiio)€thyÍ|''iV-{4»by<lríixypb«iayl)“2’'««5íl*yl”5«(6*{|{3j|^ 3' m€4liyI--3,4Hlilpvdri6soqi«!íi4«i"2(U7|-}-Í¡c¿irbiOiyl|-' 13~hs^iKlkmk-5^1)9y-{ I -meíkyk I /#-p>Tír*üM~yi}~Í l7-pyrro!i>3-<iirÍKis;iio>«k> hy<lro«york1$ íhe pxocedufc is in uceordanee with thc pn secas oí hxample 26, replaemg the methylamme sn 52 ep R hy dimcthylamsne.
Eiemettfaí wit'rtíetwtysh: {%> fheoretkaí;memw$0 %<J:::6S.46:65.07; %! M5.92:5.K?; %N-;2.05: i2.06; %O --5.03'S,55
High~r(*.u>ÍuH&amp;n mms s'fmirwmay (ESl*): .Eíppskdai Ikmiola; pM-Hff ealsulafcif; iél JilPCi (MHlf sneasured: 661.3645
EsamyPt 23. 545->ChÍ9ríí'«2-{|0^)-3''iiíieíhyl>3,4>dibyclroisoq|uiaoll8'*2(IiElk ;y1!i'arii»ip3|p6eoy!}-'A/-4iÍ->hyi¡rfwy|4ROiyl?--I72-'i6met6yIx40(pyrÍ€ll?o4''yipi2l-'pyrrole~ 3->e»rt.*«sí*mlde 6y Proe6 !orUS« m$£&amp;>eneíy$te: i% ik&amp;tretkaltmet&amp;ureá)
mms speemmuvry 'inj'>noai Vt^eda' ( ,.!Β:Γ*\'χη |Mífif eaiotdated: 591.2157 |ΜΉ I j'" measured: 591.2) ?S B&amp;aaíole 29. dihydrtóogiduolkí~2t'! 7^-yljeaHm»y0phe«yl}dV^4-hydroaypl**»ylH 52-diimdhytd¥~ pl*e«y M 2f-pyrmíe*.Vea rboxaas ide hyú roeMoride
Jfep ..:Ü> M&amp;hyl yífmrbanyOphenyi^i^dmethyi-íIi^pyrrMi^i^W^oxyíme
To a solutum of 4.9 g oí th« compound of Ptdpaouon 1 iía 8 tóxlurt oí 40 Mr, oí dimoihyHHfmamkie and 40 nú. of ielahydroíurás Mere ios sdded 273 g; (1 1 eqoívateds) of t3$-3-hp3mxyme*hyÍ“ i ^.B^-teiraltydmh^AoKip,. 1.95 Λ oí dnsoprq^de&amp;yf-ilmino a«4:; :S84 i f!,'?:' equivalems); of MAlli, Jdíet stimig Mr 2 fam$· M 'MÚUmi lemperainre, ífc reaeüon mixtee i$ dried, íaken «p m elhyl acetóle and then waslsed wñh í0 %. sgueóos mtm' 'má soleten and ymtM,. ite* mmhi^á aquetes $jba&amp;f aí^ oitraefod wilh ethyl acopie, lid ©rpníe piases iitéreby obtened «re eombiaed.: dried oyfr Μη-βΟ^ íltítex! and eoneenirated p^'gpdnf^pd^iiev Ilgeriste pptuni· ohtónni ly etteiteqgpigby: over sdlea ge! using diehlorontehane and eterno! as e! usáis io yíeld te díte oompoumf SH NMIl; i (500 Mi-fe dmso-df»; 300 K): 7.6-7.4 (m* Bl Cl-Ph); 7.2-6.9 fe 4H, Aríl'fllQ); 6.5-6; 15 (broaü % I B, ?>ymde); 4.0-0 J # d. 2!L N€B? T10Q); 4.85-3.6 (m, ni. Ni n mte>, 4 2 4 o pn, ;n. οπι^^π. * 4* 5: <vte ni. n γμ,ι un >*» 2B, ÜOCIB); 3.0-2.0 (m, 2H.THIQ); 2.5-2.! (m, 3Ü, C% pymteK i..25-1.1 fynf 11(31¾ ester) ΠΙ; v -OB Vite an'1 v "O O' i693 ern's tyoogqteed oslen. v xte v h>20 cus 5 túmido* M^0á&amp;^uimim^20M}ψí|mré&amp;m§0k^nyi)^J^iϊm^$kyi~iHψyrrifí^^ari>fixyim A solution oí 1.7 g (3-2 .nundt oí the eonypntmd oht&amp;med ñt tito above Stop ¿o 20 mi., oí dimeihylfonnamide is added, diopwix <d 0*0, lo a suspensio» oí 0.280 g oí Mal í 60 % \n olí (2,2 equivalen») m LO mL oí dimodrylÉMiamMo, Afbk Mñdng íor 5 mmyíii g| oídblom tempemfbtdt sosppslon oí'0.68 g oí 4»(i-kooíoodyl|ÉíorpíioHoe hydrobrbmiáb (I I oqunatenLs) 5s added thopvd.se. I he Harneo mwture o- síirred al amhíem tempera ture ffm-4$ minutes. ¡Tere are tlten added. i« uvo stops over 20 hours, 2.2 equis utents oí 4-í3-Umnmethd'hnotphohnc hx Judromok, aro Oteo ' cqun a Ico 5 \ ot Mal! 00 N m od. lo reacuon nusture ¢- dvn pouwd mto a nnxture oí 10 % aqotous ammomum ehiorido and ethyS acetato. The resuhíng organie: pbapis siteeessiveiy washad: with xtdkband then with '-'ahoted amurcos bit i ¡ Intica and bou? ft ¡\ thcti Jried osos NaMhK ídtered and coucentrated ondee reduced presione, .The oil Oblaiged h pnrllkd by dhfpptaipgrapby ovar sihcn gd asiré Jtohloonnethanc and eshanoí as donato to próvido the expeeted ptoduet, Ή Ntttt. 6 t500 \íiM diase-JN Tn-Tt (tn..3¾W% W4MMM*: \.ί(ΠΗΟ); 6 '-o is s rnad v III. osmio): ' 25-3.8 fm. 211 NC!1« miQ); 5.05-3 75 un. Mi NCH TMIQ); 4.2-bu (m, 2Hd K?H> o,0or). 3.5-3.25 (m, 311, N-Cii); 3.6-2.75 (m. 8H. OOb morphoimoeíhoxymethyi). 2.05-2.05 un, 2h\ THIQ); 2.55-2.15 (m, 6H. NCH, sno:nh.dtnoedK>\yn!>'únI>; 2.5ς- ko5 (!n, Mb t 1JU pyrrokM, 1.25-1.1 sor, iií. OH, ester) 114'v C m; lb04 > n>! H cunase,ed o o-. 1 x o' O H>24cm 51 anudo'» )|&amp;y? (j S~{S~ChÍmú^20¡(3Sh3-{0^mrphiiim-4^l^k0^^ky0<-30^^m-iM!tíuimΦϊ~2{i¿íl·'}4fmrh<mγiϊphm0l·h2~íimeik}i~i}$ψ}rmί¢~3~ei!?ht^\yiiCiUid K v>l >boj o ' 4o ' Ί Oí 4 oroeísond ot Oso abovo Utep na s mL, obdmxam theo' ate added 5 »nt ot IM ousuentis I sG.Í 1 soluíam. 1 he batch is heaied m a mierowave appanstus <!0U W) at ÍOQX íor I honra, Tito ruaction mixture is pohred mío water and titeo exttaeted with ethyl eiher. The ethersaí phase iá extraehsl ugain with LO mi oí water, fhe aqneous pfeas aeídílled io píí S-6 by gdding seturated igt^pns ammtínibtn chinrlde sohgiop ar.d thrn eetmoted t'w**e x^th d'cbkxonnethane the .ik'ldomntedatne phase ts dned otes Ma'NtV M teted and v - r ovni oda t; te dnoess the "nto prnduet te obuonod m tbc fono ot a morí agüe. s ti.- temad m, «a. ei
Ph); 7,2-6.8 fm, 4¡l Λγ(ΤΗΙΟ>; 6.5-6;? (bro&amp;d 4s> 111 perrote!; 5.25-3.75 {m,¿H, NCH* hoo o* 3 u» \ci i*tn>K ^ '«i \ u , **' ot'í m-te'dsoiu'un.dvvimotee1e 7,5-; ' -,e ell, NOll· rne^hoteuxOem·'metetes: *íli' no, 211 nilOi ; "-:.0i-X31UIl?nu ic> IR? v -OH: 330112200 em':, v ->OÜ: 1697-1662 cm'! (double barsd, eatfeoN.ylic acid}, v ''(.Μ.}: .1628 cm'! (amkle) (m&amp;rp&amp;&amp;iw^-yi}efitex?jmethyí$~3sf~3ifiyrfr&amp;i8(*qi*ttt&amp;iíit~2{í&amp;)-?ífc<tr&amp;eñyíjphen¿>i}~l¿'·
The acid obteigfd m lio?* 0 ü,48 φ |$ suspended in 15 mi of 1.2'dk!doFObt|anev i 1 mpev.deutete i-chlmote V teñmelln lpro|vmhem«e aro added ihereso. Ateo sbmng ter ] hmu ,n mu hete tempe? tee?., teere are added 15 mi. of tolueno and 1,05 equivaléis of tho eompbund of Prqwadon 2'\ The reaction mixture is hented Ibf 14 teute ai ItOflr and J.hen drsed ihe cuido p vía teemln obou ed e, puntee In dueouUogtepte over liíim gal using dkldoroornsbane and eihanplesblnante te yield Ose especie#pntetueu lE NMRi 5 o» * NHL ínso do, 3UOIO " Ό ' 7a (m UL c l~Phg ’ 3' <‘6 ge. <HI. A *JfUte!lphen>b, 6.6'6.5 sm. 211, pbenoxvt: TO-n 6 (m. 211, phenoxy}, 5,7-5 05 tensad te-, HL p>ue!te: r 2-3.6 |8d, 211, teJÍQh 5.05-3.0 Hte, 111, NO! ÍIltQA 3,6-23-> |m. 8H. alíphabc mnqrholmoethoxymeihyl}; 3.4-3.2 (broad 3$,. 3H, MÜH(}; 3.0-2,! (m, 2H. T1 IPdl 2.35 (m, 211 NCHj morpholinnelhnxymeihyí); 2.4-2,2 (m, 4H. NOl morpholiüte; 2,4-2.15 (broad 3r>, 311, Ote permite; 0.8 (broad 2st 9IÍ SiCXUI.te): 0.1 6ls, 6 H, SiCH.O lili y ^Ctel3t 1635-1595 cm'5 (douhle babd), v Si-O: 1117 effr , v 81€; 837 orcf*
Afeo 12: 5-{$^&amp;fo¡^2~{f(X$)~3~íí2~(m»wh#ftn-4~?Íteth&amp;\yjlmethyi}~3¿~(tíh)iár<*&amp;»~ qisim>fitt~2{ni}~}4jcúrttmi4fphenyl}-N~{4~k}&amp;*typÍienyfy-it2-iíim#ikvl-N~pheityMH~ pyrmíe-3~mrbox(tmMe hyíimckí&amp;mk Té a aoludon uí 0.7 g of the componed of Stop O 1«. 5 mt of merhanol Ibero k added 0.92 mL oi a !M M>lutíon of poSesesum hydroxide in unOhauol, After .stlrñng íor i hour b' nihunu' at m.· tmm¡< íSatonited uu κν»Ν sodium n carbonate sobnutn w 'M<\t ! he padecí v.hteh ¡n cu pósteros Osirteu d wlth oih>) cáeme,· a«d Oten vutshed w jH water and brote, Ihe retmllmg aqeeaus pitasen are extractad agam with dhyl acétala. The ohtaíned »ι;ο dtuéd over Ma^SÜ*, fíikted and: éoncentrssed umler redueed prrssure The cruda p rodo vi ehmioed is puriíted by ehrootaíograpliy ove? süiea pe! T|tgg. dlebldMutMbáss: and anuneaíadu-'etbamd aa elpatis iopwtdb OJp g ef (he (lile pwvuHl. ut ihe tenso os tno e_.se suc tatú; >« dasádud m aeciu^utnie and s no ves mu ¡mu salí toros nsutg LM .κμκ'Ήκ Mr 1 seluunn \brr a hopbíbsaüon Mep, tbe especiad pnxiiscí rs ohtamed ós -he i'o.rot ni a ardid, MMmámiM micrémá^ÍMMt0É^ mi^suméÍtfimrefkraí}} %C:;kib,4(66.?5};%l‘b'5,9Bpd?k%N:::? JSi7.4i };%(M:::9.42(9JS);%Cr::::4.5?i'4,69) laoniude 2(1, 1
SfwAí ¿fiert-BunifMmetfn'í}7(v7/í<X}iph<and(í-md/iyí-(H-pymzüt-j- )fy'erhemofíj-¿~metk¥Í~$^~Íf(38h3~metkyÍ~3^ib}^MS0quinoíW"2{Íílh lifcer&amp;Mvfn'^tetnettwxiti^yihífi-pyrrtti-i-yíÍi.'íkyÍ methent'*HÍphmúte A soiniktTS oí 4,6.5 g. oís he corepoand ohtained in Step B ul hxampte 23 <6.2ΙΜμ©|;) ϊή ufe -ofanhydftíus ΪΗΓ h placed ai if'C. 'Hiere are sucemiveiy suided thereto 3.5 mi.». (12 44 rundí oí iíxeth}!aennv and liten, <lrepeasc, s> ' 7 ¡s>l. oí methssnesulphonse chtonde gí 31 uneoM d»aM»btr- m 'id ni' Η Π i 1k bu oh w dn n 'ií”cd a* uiepent ttsssps'ratussi p,¡ 5 poum 1 be reacnun "e'Uun n. hub »wsed h'- aedttmu o- aatuuu.3 aeaenm balICOj soiu&amp;M-É&amp;á thes cxlmeted Uvice wph eiliyi acetato. The óigante pitases are eottshhsed, dried over Mgbío ¿5m' votseentraied m drenase. Ote especiad compasad a* obialned Ib :fc foros oía glassy so No, *|| NíVSR: 6 (500 MHz; amso-dd; 300K): 7.7-7.45 (a, IIL H pyrasude}; 7.1«6.9 (s. IR II pvuosky 7o4 {5r 51> tsurcO > íst> (oo oA < ' '1 a eíVtaU 1K> el ot H 0€íl>0p 5.2, 4.6 (4s. Π, 1 bpyrrole tpreseoce oíco¡nf(»rmytioual isomers); 4,0,4,6, 3,4, 3.8 («?. ΙΗΚ 5.05-3 75 (m, !Η, aiiphsue R, H lekahydjRsiaoqumoline); -1.3*4.01 (m. 2H. aepn me Ha x H C1HOSO <'H k 4 5 χ s>s , ,\ HI akpkn *. k\ < llg R mku i 1 * ' ' . 2.7 i2s, 3!!„ €íH'-pyra/.o!e}'. 3.0 (severa! s, 310 €H49>SO¿€Iii); 3,05-2.5 (sesera! un >!!, aOphaoe IK Η Km o h x d' o s \oq xn no h ne), 3 45-2 ) (.sexos ai s, HI. (Ή* px roles, f 0\ O ?s (severa! 0. ML CíMetraisoqnmoime); 0.0 (severa! s. OH, Sí((2íl5):(£3H{C.'Bih); 04 (ms 0H.S4£ll3h(CH(CHn.4 IRí v: >0=0: 1626 em'*, v: -SO>; !349a»d 1172 em's
Sien 22; /--(2- 2rñ/md/p0-Λ-(4-¡¡ícH hutykdmeihvihϊi}i¡<^χβi*he»}'i}~2~meíhγl·^{6-^{3ΗΙ~3~ΜΡίΗχΜΛ'ΛϊΗν0Μί\&amp;μώΗ»Ηβ~ί!{($ϊϊ'?ί&amp;'β?&amp;&amp;8νί}~^·$'ϊ*™?&amp;3Η*χ0ί~$-}'8~Ν*{ί-meÍhrf'i^\p}TtiZ&amp;i~4^hlH^ym>k^~mrhoxmii3e (o a s.4en-vx K>\ 2 y, «2 42 mino!' o! aimpound oí' Slep Λ ín 20 mí., oí anhydrous OMF there is added 0,472 g (7.20 rumo!) oí andana a/ide. The baíeh !s síirred >.n 6543 f'or 4 botas ,χΟ nnrokes She R.xom; ;m\mk r ]H>med mío 'aranaed amn-ms Malk'Os áoknion and dien exnaeied ίχχκ", xvitb odpl aeeíaíe ike órgano piases me eombmed and xxasked. o Oh ’euos.nrd amaneo ! 4 seiukon, dnx\1 over Kk>0.t and eoacemraied so dryness, Aíter püritkaüosi over sí!lea ge! asina dk'hlouxmeihane and ammonía-ím meihanaí. as eliíarus, (he expected prodne? Is éktasnedin ihe íorsn ofaipmn, %íi NMH: 6 (500 MI!/. emso-dO; .100KV To5-7 4ñ mesera! v Mi \\ p> rumies; 74.-0 0 (severa! s, !!!, H pym/deT 7.3-6.4 (severa! m„ 6R aromaüe Ha); 7,1-65) (severa! s. !H, aromatk' Hsk 0.8. 6.5 (2m, 31!, aromado !hi; (>.! (severa! s> .3! I, Ü€II>Ok 5.25- 4 o5 (severa! a. !!!, íi-pyrade (presessee oí coníormatiooa! iaomersg 4.9, 4.6,3.8 (severa! no nig 5 05-k'' (sexera! sis, 4|{, aOpívko Hs. !!-!!!!() - €!l>CH>ks:o 3 '? mexeoo o m. H íl!e-pyra."o!e! 3.5-3,35 Ou. 2H,( !K ION;, r 34-7.4 mexere! m. 3H. CHj-pyrrok*}; 2.45-2 ' \ ..i v. HL <ΊΙ.-ρχιτο!*Λ ! 4 5 '» (sexma! o Hl, < Ηχ llllkk >ou.í s, !k!: 3be Ηο>(ί'ΜΐΓΗΛ)ίν.Ο ! (sesera! n.w| S-.prHjhíí HK'Hx*a IR; x N \ N 3l00<m ,s.'4' x> k4 i m 5, v; ~vs O- OH^eni dikyd?i>i$ú(piin&amp;iín~2{ ÍH¡yi¡airémyii~ i JdHmzmiwxid~byi)~iS~{ i ~m€íhybi Ηφ%^ζΜΦ yH'dH-pyrríde^bmrh axttmub Iíí 8 solado?? o! i.12 a oí Che compoo??d obtabred io ¡step B i i4.-19 romo!) is; 5 mí. ot moderno! tlie-re -.xv addod, dropo-oa, 7 24 nú oí IM uiribaeobo polas dnm iudsosKk-snhuton (72.45 mmoí), The bate!? 2 stirred ai ambieni Sempentture k?r 3 hours. Too reaebon mixture ?§ thes? eos?ee??anied jo drvness. tabeo up in did?iorrm?elhíu?e, hydroíysed wuh sat ura sed aleona Mal ¡Ci u .soiumm and ibón extractad tooee uhh dkkhaxtmethaue. 1 he orpapíc pitases are (he?? eomhineé* vvashcd witli wbtbtv dried over MgbíSa a??Í coucenoated lo Jryoeva lite capot κ d prixintu !\ obtabted so (he fono oí?? iba??:? and osed direcdy ir? the ??exi Ntep, S1I NMK: 5{$U0 fviHo; dmsn-dó; 300121: 9.6¾ fs, ili CHkTbOIi?; 7.65-7.45 (severa! s, I Η, H pyru/o.U.·}: ? i-6,9 (severa! s. 1 Η, Ι·ϊ pyraxok?; 7.3-6.4 (severa! np 6fS„ aroma?k Hsy ’ 1-6.9 («meso! a 11 í. atorune lis): 6.8. 6.6 ?2?η. 21L uromatic lis): 6.15 (severa! k 21:¾ OCHjO): 5.35' -LO tse>,erai s, 11 ís H-pym.de (preseuee o!' eonfnrmaiiomd isomersV, 4.9, 4,0, ' 3 mesen?! ,n. li!. abphane i!, il ieiMhxdtois<xpm>ohf?e' ' ~ s set eral s< 511 fl V\lA~ pyra/.ok); 3.5-3,25 (n?, 21¾ Cíh€í¡bN-b 3 1-2.4 (severa! m. Hl ri!¿-pyno!e): 2 45.27 (severa! % 31!, ü Π-HQ): 2.45-2,3 (severa! s, 3R CíRpyrrokp l.0-0.75 (severa! d, 31¾ CHi ΠΠφ IHí v -011:.3471. c?r?‘\ v-N-N-N-2!00un L v: ' ( '-O: 161? cma
Step />r /-f5''4.ou>oa-t/n7)-A'4rP/n'2rovF/0oun'/i'-2-'euí>f//r(-5-{6''}7(57^'J''t«¿P/p7-'-i14-· diÍn^d$0ipbmdiP~2{íHhyfií'arb>mihiJ~ketnmibxid~S~y(hN~{f-'meihyi~$H~pymzid~4-yfh lH~pyrmie-J-eurhaximhk hydravh bride
To a sobado;? oí ! .27 g oí the eomjxnu-d obtamed in the above ¡stop (1.63 mmol) ir? 15 mi.» ol ¿nshydrous etharml there is aelded 0.154 v oí Pd/C Í0 %. The baieh is degttased lor ?' 5 Ix'Xts, !( o íL'í sO'kd 6a :s kms a? smtnem umpeotUne under hxdrogeo ps tasase (75 ¡un The 'oacttoo srjxüso, sx kxm fbtered and theu eoncentratod m dnttess Aiter purideubcm by ^hrornatographf ϋβ sdki: pd (Clíapl^MeDH/NHs gradieol). cm ody residuo is ohtamed. 3¾¾ residuo ís disssdved lu !0 ?«L of asihydrous ethmtrd and Ihesr converled seto salt íom? by aUding ovo molar eqnivalents of a !N solado?? oí !!€) is? TM próduoi Is ftevdrbá hdtes b#hg. dsÉsPkM I» aMnlaiúid »f á mixture of MUar asid aeMoPlltda. Aft&amp;f-n lyégMll^ItmLskF al low lerapanaure, fhe expected prodticf A oblaínod \n the Toras of a
Eiementái mk'ráü&amp;afysk; f »> íh saralícaEmemureé} iiighremkkhm mass sp^íinmt^iry i ES i *}:
¡kmpsrkai Sbtssnsla: (AíJA^AA {Μ+ΗΓ cakulated: 633.2820 ίΜΗΠ' rneasured: IA3..?.K0K
Emmpk; 31» ¿\-{*l~¡ly<lr«xyphenyIBS^SHmethoxy>2·{í^é*-dlhydroís»<|«m«Ííti“2{ i77}-yí|carbom· i} pb^aylp 1 ,'2-ífimtd feyi-/7-(I - ma t iw I- i í.Epyraaol- 4-y l)~! II-f/yrrole-3 ·-« a rhtt xa m kia
Eiemeníai mícmmmíysk: {%, iheeretfkatmeasímíd) ΜM !V29:?d,! 7; %TN5,9l:5Jl; W-1!Jt:! L# Mígk~resoÍMiiim mm¡> speí'íntmeírx {íiSfi}:
BrapErk&amp;l
|Molí j' galoolatgíl: S0CK2702 ¡Mhá}]* jHgfsureí!·; S30.277K
MmmklL Alance example)
Fheuyl (4'7>E^TI~hydros>:|4His:syIi(«H3hyÍKair0araa>4|-d3H!i«Hah>3' !ll''pyrrái-2-yl)''3·' (j(3l3'03'asiallp7'\3f4''iidi5aisa0saí!OÍayÍm\3{Íl;f|-yl|iairlMaTy00«aayl)oar0aB5íPa
Slap .-Ir N"f^MmEvÍjm$pke&amp;vi}"SJt2"mmetk^pvr?o&amp;~3-<m?itm®miék The psx>cols is aaaiogoux |p:th&amp;t::M Stop A ¿íf Baaaiplg 4S,
Ste» B; tereButyi i4- (4- 4-1 z)4myjpkvmii¡ m¿fkyi)effréemityi}~ 1,5"iümeihy^$ H~ cerbematg"·· l'he componed oí Preparaüms Lí i 1.5 &amp; 2.96 mmoi} and the componed ofVStep A ÍLÍ6 g; 3.44 mrooh ore dtexolvsd so dk-oeíhs hseenirsude (25 mi s and thess nitrogen ss btsMbed thíougb the seísmos* ibr 5 smnotes. Potasssum acétate í0.5M g; 5.92 sronol) and bisüriphc$'i>dphosphme)paIíadunn(H> diehSondc (0.2 g, 0.295 enees) ere added to ihe mkime \%hjeh te shoo heatod te IOtV-'Γ erd. atter stsMng los Ό minutes, ssater (te ul s 0¾ added. Sítense.' al 1003 under a nitcogen atmosphere te coivtsmted for 2 hours ers. l'he st\sosmst stóMno. s-s altee ed e coot m s;omestt tenspcnthsre and sn sisen evqvraUM te a \oksme es' 15 sol l'he t'essdt'.ng msvort <s Hiloscd o\er a ohert 1'élite ^.oltisms and rhen. puriikd te prepanmve Iid: t ustetg oater~U'A ard acetenonte as eleaots she psl 01 sha approps tase tsaenosss ss admite? i 10 2 v.eh Sal ICO 1. and Osen the aeclensaik ís evaperakd off ustdes reduced pressus-e. i he aqueotss resulte; is cMmcíed tvteh DCM. The organic pítese ss wa|h#i: assd evapetated te drynoss te ysetd tete üüe eenspound.
High-mseluSkm mass spedronietry í'IRSJO;
Hmptescal formula; ítedíteteDi |M*H]' calcúlales!; 7BJ70S fX! a H|' measnsvd: 713.7709
StmC;S^rA^tmeώf?fM/¢Ml·^etéi^i^ik.van>J3^^^ittaβ»-2am‘Vίl· aírhmn4jphi’ft}4f~N~i'4^ett^Íüxi\}phem4f~NJJ<Mmefhí4^íly}>m}ie~3~em-bmmíide
The eenspossssd ef (he chave Stop (1.1 § g, 1,66 rosno!) ss diesolved/stsspended m 4M HO in dioxane (20 nsU. The heterogeneeas mixture is aterrecí for 30 mis tutes at troshsenl temperasen- and t|«« rx.ghuaU'd se 4s>ness te ¿seid 1,19 g el sise hite eompound whíehis used so she stexS Ssep cabero purefseastetu 2>; Pheayi Ui~-pyrmi-2~
The tille coroprnmd is ohtamed marting irom íhe eompound oí íhe above $*ep lo acconknce whh íhe process ofStep D oí Example 45. MM&amp;mtiMl miÉmmíiiysÍs; (%s memuredíihmov/é
High··rexoiutfon mmx xpectmmatry {&amp;Í*)°
Empírica! lormula: C *<>H í«N^( e jM+Hj' calcuiated; 643:2922 íM-r Π]' measered: 643J9M
Eviii|ml&amp;i 33, $4f5-€^!mví“2-ffí3&amp;14Mmoyphols»^"v!meíbvtKM-dlhvdroisoí|«i**«IÍ8~ «V*sssssv>sv*w«JSyii%v«*s.'«ssxsw«· νΐ >· .< >.· . . . . ¢. .. v ** .v .* .y 111' §>y mdeT -e a ritos ¡«a i de hydnK'fdoride Mk^m$&amp;lmfárü4MÍ0h: {%, mÉmmi>é{ikmmiÍmí}} MTCM arO'''l'd|í3S}'34?e.ia'ple:drn'4»yhHetlyyli'v3,4''dihydreis(a|eÍnií1ks·' 2íll#)*yl|e8í'hrí»yÍ|pheKylE/V-(3»'lluí>mphe«Lyl)''fV'-í4-hydr<«fyphe«yl}-l>2”dími‘thyl~ 1 ff-pyrreie--3-earfeo su* míde hyd rachíorlde
Eiemenmí mkrüanafyxh: (%< mésiMmé0Bé&amp;MMmi}}
Es ampie 35, irderaaee exnmple) /V«'{4~Hydí'«sypheayl)-Af,!s2~íHíííeíhyí~S''<2“([(3^)-3»methyl“3,4-á*hy<lroisoí|«i»«Nis-2í 1 //pyl|carifed«yi|:4«| |í |>li«iioxys«atyííaiaí»e)methy ijlphfeay I)-1lí-py msk'-3~ e&amp;rhoxamkíó.
The tille odmpdeid |i odtiíied t« secerdMce: with the procese of IxdPple 32 ueíp| % phenoxyacetyl ehlorlde as aeylaüng aeod m Siep D,
Bfemettkíí imcrmmaíysís: {%. tmmuresifihéúmiimi})
High~resí>Íufkm mass sjwtrtmittfty (ESHj:
EmpiricaI formula: €4¾½(h eakadaíed: 657.>079 l'M-í-Mf measured; 657.3061
Ksamsd*? 36^ i rufbrcocc exampic) S»{d~|^Eíhyk»rbam»yí}8mm»}i»ethylÍ"2"||(3.^}"3-'metiiyl~3í4“<IUiy.d|r«Í$í>í|«í»»Ue-
2( I Z#>~yi|«arlw«yÍ!|Ífe#Rj1)~Ar«f4~hydr»^fh$fíiy l)-“iV»I airbosamlkle
The hile compoimd h obtamed m accordance with -ihe procesa oí iixampse 57 usíng e?hvj ísocyaoatc as acylalmg ageni m Stcp I><
Ekmmmi wkroimúíytist {%, memurt*d{th£orttfk'a$$ %C:"?0,8B(?0«S);%j (-6.02(6,62);%N-11.17«; i 1.8} máMs^eéirúm&amp;ip íi'Si * i: kmpiríeíd ion aula: (ykbvNTB |M723]":óák:ük«rl^4.3 ISO [K^fl^l^ nieasaresl; 594J683 EMmkMt rraOaancf; exampk*} ;5„(4^.yil;k-n/.>kasrl>¿^?na;\l)íni6ftxí]oHa6yl}"2~l!(3l?)"5l~^cllpvl"3í4Hllhyí!raiao<}o0ialia·' 2(17i)''yI|í:^rbaíiyi||>hí;nyI}~íVd4'd5ydra.syphcnyl}''.Af!',k2~írl«5c?hyI''I7í'-pyrs'«k"3·' carbai.aroíík
The obe aornpound is obsahsed ln aaGíjnkm'e wlib Iba proeess oí Bxsnipfp 33 oalng booayt isocyanate as aeylatmg agent la Skp B.
EfetmmfiiÍmh'roamtfy$i%; (%, meesuredfthéiiréMMi}}.· %€······ 7 3.21 (73 Β6ΙΒsíl-S #8(&amp;3 };%N :B 0.2 3{ 10 J8}
Jfiígh~resoínUúfí mms spedmmeíry (E'SH): 35niprÍM! i^réiülaí 1¾¾¾¾¾ f M TI4 |: i'iíi cy.kiisí!: 666, 3 3 IMrl-lf' metoured: 656.3256 «arboxsmsde Eíementuí miermnefysh: (%, memnreátíhevreti$%i}]í %C^?0$7<?j ,34};6s!:6=E J(5J2);6&amp;N::-éJ6í6iE3)
di!sydrt6siM.píInolifi'2{lll}~yl|€5irbí«nlE^lí3~b€ni*#íÍÍ<sx#i~5"y!)'-Aí'"0^iH6:líyi~iÍE wi£?&amp;efteiy$h'? (%> mmsmed(theo?eíicul)} %C>69.6 ! {66.64 K%l 6-5,6( 5,51 κ%Ν-1! .55( Π .6) iUgh'-resohiíhm mms spedFmndfy {E$I+}: IS®pliÍM! Ibrmula; 1' *s!-)j.}HjOy ;|:M+Hir'-§ulcuUut\l; 604,2554 m«s$ure6·. 604.2565 v...V'^Ñ". V'.lv,.' v.%66,.^-. 1^../ $tep Ay ¡^~(4~Bent<vhixypht.,íiyí}-'.sS!>Í,^íi'¡meihy!~pyrmk,^carhoxímíMt The pmcess ?s atialogQU? to tltal describe*} m Step Λ of Exssmple 45, i o*6<* a iÍpheniif'S i^-{hen:\h\\Ipm ,?t */ \ ' ' *r >m^í$~$H^irmie~3~em'h}Xímíiée hyámckimMe
Ote comportad oí ñ'epurahoo III (! ,84 g; 3.99 mmoi) and the compmmd of Step A (L60 p 448 mido!) are día|dlved la diiietliflaeeMmMe (26¾¾ «tí- Ííéé hitogen is bu&amp;bfeá itengh the soluíion íor S minutes. Potasaiam acétate (0.78 g; 7.48 mmoi) and ^lríphetvyI|>l3os|#ii)palká:í^ÉÉi||í)' diellomfe p.28 g; 04 mrnol) ara added ti Ibe mixtura whích is then heded to 1055X? and, altor siirring fot 10 minutes, water Π1 ¢.0.,) ís added. bihsing al Í0.V‘C under a nitrogeil atmpsphere A uomi-úiátí !fdr 6 houm inore, The reaetíon mixture is a|l0:wi|1 to eoof lo amhíem remperaiure, the resuíing mtxpíi te f)!teml overa shorl Ceiüe eoMmband w thenpuriñed by preparativo BPLC usir%iiwUter-TPA:&amp;ÉÜ aeetorntrile nsi Pluants. Tire: p!isof the apprppriate irplbns is najusied to 7 with MalICOj and then the aceioníOsie ís evaporaied otY under tedueed proseare. The xolid precipítate te removed bv liltratkm and thendried {5 tobar. 45°C\ 16 hours) to iorm &amp;rAbutyl f3d4-({’»T |benzy loxy )plmnyl)(med5>· i icsrbamoyff ~l,;ó|tpeihyi -1 ^pyA(4-3-yI)4''{|p^|4-!bMbyf~ 3.4-dihydmoxx.unnohe--2(1H}--y|carbooyiT^axy1parha.ntaie wbieh ss dbxolvedAuspended in 4M I1CI in dioxane (35 mi..). "lite heferogeneous pkture w síítied te- 1Φ minutes at ambtent teptpemlore and then evapomed lo drynyss |p y!pli tbe iitle pptsptnnd whibltA used 1n the next Stop wiihout purifir«uion<
Iisii*4eyel«tiór&amp; trm.m apeetro metn? (ÍÍIT)t Brnpbieai íormuhr. 0*4 I^KiO.·* 1Mi Π[: ealculated: A13 31 Hí? (M3§!.f rupamsied: 613,3104 IB; v:: N4I+: 2854 enP; >0=0: i62! ent^pCbOTT 1234,112|5101! em4 Síep O Pheayí i 3- f4~¡{4~kytírti\yphmyp(tnetkyí)ear$iiíthoyfj~L S-!ÍÍmefhyl-ÍΗψχηί>1·2· yf$~4~{i(3$^~mMkyí~Sf4~áÍkydF(}M^Hmkm~2{Hfl^ÍimrbmyÍ^ew^Í}£m®®m®te Tbe dt.se compete) m obtaiped starüng ítem the epppouui of the aboye Stop m aeeordmee with the proeess ofibíp D of hxatpple 45,
Efemeattií mknmmfysis; {%> mem-medfthevretfcafy) fíigh-i-emiutwn num %peem>mHry (£$$+}: lmpmMtfeOT¾ιli^C¾ííI½¾í^i¾i}5 | Μ * I! f calcúlate*!: 643 .292 2 jMHif meamired:: §43,2902:
Exampie 41, íV~í3*Fluerif|>heny1)-/V-i4-fe>-<isroxypheayi}-1 t2-ú9m'tfsy§S~fé~(|íM)-3~ 0H4hy§3,4-dihy9rf4;soqiO?M>ll^-2i¡l/}“>'l|«^rb<í«}4hi3'4>«»EtHl¡oiíO'\4'-yl|-4l§-|n'rr€í§.''' S*€arbox»mide h'femeütei mtcroattalysist (%> memuredfiheatef^^} Hígh-resaímiún mms spenromt^n· fE$Í+}<'
EmpfnMi IxmOulO: C&amp;lfeENaOs ÍNRUf eaiculaied: ó 18.2399 (Mttlf measured: él §>*392
Es&amp;amls 42> N~{4~Iiydraxy phe«yí)4,2~dimeÜ»y 1-5-(6-1{0¿?}k$~methy i-3,4~ dihvdroisoí|«iPoiip’*2{ I /3f}-yi£c<*rti«*tyl|~ 13*6£f*£odm»i-5~yi)4V'CÍ -methyI-11/-py yr Af 2 >3M> 1 P v r |d I n -5-yl}« I l/-p|pxr§le4^yarlj0.xaPlMe
Ekmmt^i mkwfwetysiu {%, meamrgé{fheore?kai)) mom .3:2(31 ,§5);%Β-5:. 12(5 ,%%N- 10,63(1 ¢21} iligh-rewiutkni mes* .hpeetrometiy (ESí ''}:
Empírica! formula: CVH^NjO* |M vMf calcúlate*!; §54.2711 |M4'Hf íi!i:d«mv4; *tM 3710 <llhy<lr»is«q«iii«i|¡»-2{ll#>'yíÍ<í8rfeftiiyl)‘' 1í^yrrek'-3»
MirnmmM mmr&amp;rneimis: (%, memmetí(¡theoreikaí$ %073,9(74.1 i };%!f-=5. l<í(ñ.5;w:%N-O1<7.01) fíigk-reseíutimi mms speanwu'Ey (liSl-t); inunden! tíϊυτϊιϊ:4.s.; < dKsíbO, «v hi » v.uj,^a 000 24^ 'M *i|' -'Vínuuj ouoaa^s F^asttjile 49, 2í49~My dimypiseny 1 y 1^2«<lssiie$hvl-/V-(I -metliy 1~1 íí~pyms 1«|&amp;3~&amp;|* py r id 1o-5-> 1 p 5~( *H t 135>-344nerpkoHn~9hy I rnetfey 1ρ3,4-<Ι ihy<tr«§ísa<$ «1 nntin^C i H}- yiJc&amp;rfMnty l| * I3»hea*«di8x«l-Syl)· I íí^py liydrociilonde £iemmteí mkreMMpi'sí (%* mee $ emé0¡émefimk¿ %C^66 Jh'6ft.62};%H-x0Si5.5O);W:4í).85flO.84^%C:i.==4.68(4.57) íÍÍgh-resii)aímn mm\ spmimmefty (£SIH>* [uopmc;í formula: ( «R^N^O.·, tSMir-akmUcvi 73^32V. IM+Hf measurcd: 739,324o ilÍIBl&amp;iS íreference esampíe) 4'{!(;3t)»3-n^tl3yl-3v4~dil!yarfasiHpiUtaltu-2CI^"yl!t%^rl)uuyi||tlíeu>l|eil^íllearbamaíe
Siep.4; 3 t EBemjioxypkeHvít V(,7 tmneth\i-pim>k~3-verha\umn/e
To a suspensión oí 1 Joiin>cUpy!p>rf'oie-3-carbo>;yHc a cu! s Pifo y: lánun^!: ni i d- dsehknoethune (40 mU itere is added fi^-ehléiro-^ (2.3? mL. 17.9 .mrnoíf and the solarlo» -fomted is stirred tbr 1 horas To Ihe tesohmg ioluílon itere is added, ds\nn^se; un kemold soluüon oí 4-tewy!o\y-A-merhywrrdimd: t' ''Η- í> nuooü and V~Hhth Vixopo>py 1·propon-4 arabio {? ''5 nd., 45 sonad) m 13-diehlomathime <40 mU. The rpaeüon mixture is etirrexl &amp;t ambíent temperatura for· I hour; Si p$ then dihued wdh dtchforonKthane (450 mi \ and washed *vith water t4 \ 30 mi,\ dned nvu Ni'Mh and sunponred I he umn ewxh vi w a? trove <> T ds Ή i e ! ,n and K< £<áfdjlHMMe filletcd oíf te vidd the tirte compound. IR: \: Ή' «» * olo un ; nm de: t <08 un !' ν' O C i4Os, I} 3\ 5u<N em $¿ep ih; tert&amp;ufyi β~β~ί4~{/4-(&amp;ΜΖ)40Χ}'}ρ&amp;η}'ίβ{Μ&amp;Η)^4ΐ?Ηβ*ηο?ί}-ί,$·$*ηΗΗ?(~ΙΗ~ ρχηο^-νίϊ^-Η(3ίΟ”3^ρί&amp;}Ψ3ί4"&amp;^φΜ^>*$ίίίηί*8η~2(ίΗ}'Υΐ$<,ιϊϊ·&amp;οΜ}'}ϊρΗ*'Μ¥φίΗ),!$«· eiith&amp;tmifg
The compound oí Frepaimion. I <0.869 g; T67 mmol) and the eompouud oí Stop A <0.558 g; J .6? mmol) aio dts$olved- :n .dimethylaeetumide <8 mi) and dum «Uroge»· is huhbied throughThe soÍMort-fpr 5 minutes, Potaxsium acétate (0,33 g; 534 mmoi) and 14a(inphenylphosphtnelpalhulmmi \I} dichioride (0.117 g; 0.J 67 mmol) are added H> the mixture ohvh is then heaa.-d to t40°C and, eider sürring ío? 10 minutes, water (80 pi,4 is ud® huno ut 140 * smlu e mnogvt aínvsphetc sn continuad Xa ,>< nx ' na e le: vacuon msxune u aikxvul tu >,,md to umhem temperatura and Oten evaporand, The residuo Is partí üoned heneen diehlpm^ mL} and water (20 mL). The organsc pitase in vashed wdh watet t20ml.t, dncd o\ er \hoSO< mal evaporan?*! íhe rosüue xas pended by preparativa ÜFLC issmg waler-TFA and acero?)iinle aa ctoams, The plTofthe appmptlite tuedons ís adjsssted to 14 usmg apéeme KaOll suiution and Oteo tha aceUmitriíe is evaporated olVunder reduce:! prensare. 1 he aqueous residuo is extracten \uih dichlorometham? (2 x 100 mLy The organle phase is dried over NasS(>.j and evaporated to dryness to vieid the útk compound Hígh-remkPkm muss .’tpetirem&amp;íry (ESfr): bmmmxd lomuda (^.1ΚΛ4Τ =M t lTf calculuted: 7273861 : |® ΤΙΪ]' me a su red: 72 7 J 852 IR: v: T>0~ 1705, IMRcm'k amiüe. 1500 un'h C-OTs- 1444, uhft, 1012 um’* ¿e?tott}yp&amp;*^i~^~fó'(fenzyiúX]?)pkm}fy&amp;J,2~¿rimeik?i~iíí~p}'t?úte-3~eür&amp;0X8Íliádñ·· kydrm'hhmtfe [he eontponnd ot Su-g Ü e}J> g; 0.48 sumo!) was dhssoiv«d/sa«pe>Kled in 4M MCI ht dsoxane (3 mL). Fhe heseftsgeneam mixture 1¾ stírred Tur '30 mssuites ni ambienf' temperatura and Osen evupomed tu dryness to yidd the titie compouod wfekh ls useu ha next StC'p eerJeng heistg punded ai yií, UÍgh~n*$t*htfhm mas* ^pecfrmuiry ?leVi >i;
Empmeyí ionnsda: T jM-t-Hj' calcuhueü; 027.434? |M * H)+ ms e^ued' o? ? 35o‘s ;iK: v: C-H: 203! ero'*· >Ου: 1608 ero'*; anude; 1508 an‘!: C«0-C: 1333. !CH2em'5
Snm D’ Pkmtyi 7-0?'i4~Í{4 kyi&amp;a\yphenYÍ}tmetá}fh>arkam®rifJ.J~dimtkkrí~itt-pyrwl·· 2~yÍÍ^~Íf{3Ñ}^~meikyÍ^J^dmfnnv>qmnatin~¿{Ín)y4jYm’ktwyifpkí>nyi}eth'yi!~ rk? eomposnsd ol 5sep C (u 10 g„ 0 15 mmotí ¡a disolved in dichiosossiethane. Ohenvi ddomlormaíe (0.02? e, 0/f? muse!) asa! OhsopurpytefhyIusassm í0.i>0? g, o.?5 simio! 3 aro added tlarsito. i he seaeums sisiMsire kstsned ut anshiem temperatura tor Ό) minutos. After áil'iiÍÍM'WÍih:dígÍlé^m^ttee '(TOO mi.}, tlie orgpis phase is washstd vdth water (20 mis. evapora teri and eoncentrated. The residue ss Osen dissolved in ethanol and a PdC catalyst ss added «10 mgi. The maesioss mixture i? hvdrogesmSed a? atmospherk pressure ai ombkm íempensmre, When tía. reaeíaai i a complete, the ^atabal l\ remoxed hx fdseoeev. -he tshmte is eo¡wesst.s'atxsd and ihe cruda produet b psmíied hy ehrosssaiogs'aphy ovar siüea gol ushsg dichloromethaise a ral medirme i as dssasshs, as yieíd ihe tille pregue?. t hmnatmi mh'numahsis; (ó?, mamttredíthearetkiií}) "*' '2 ks?.U5) -vil e.!5i6 μ^ηΝ 8 13x8.53j
High •'resoiuikm mas\' spectmmHif
IsMpíritíal ibrmylá: Cy^M$0$ (M-Hj* taicubísd: 65? Jo?? '[MVif |* measurcd; 65?,3062 isaamkifes treference eSíimple ) 2{ll/)''yi|carlMi«yl}“5'~(2»|(|>hefioxyaeefyl)smi;««!eíhyij|ifeíí8yl)*l/íi:*pyrroÍ€“3-
The dtíe cotnpound ís nbtutned in aceordance sviüyihp iptceaf .pf I!|s$mp!b'4|: .pÉpgVl” ikenoxyacciyi ehbndc as aeylafeg: agein fa Stop 0,
Ekmmfñí mi¿-N)&amp;hñiy$í$t (%s -metmiredftkeoreEmi}} %C-?2.?4|73.4 0);2«H-6J8(a.5!>:%N-7.a5(0.35} Hígk-resühtfíim mas* speiirtmiitfn' (E-VI'J:
Empírica! formula: €;íd ¡«NyO^ |M J¡f eakukted: 67I.32.V5 [M-t-Hj* niaa.aareO; 671.3226
ExamMe 4?, AH4*HydraxyphenydH«2-dí*BelhyW¥-{l~m«thyM&amp;~pyra3N)M~yl)~S^&amp;* f|(VS)-3'{au>rp0s:ó6i'4-yla^lhy!}'\3,4'ílíhy6roÍsi¡íp6aaIía-2cl2l}'y!!t'arl:íaay!6'1;3'' !>ivír¿a:.a;jittxí6-S-vi6i?Epyr!a.6fck"varl.H^>tamkl€ hydtYsehíariíle
Efermaíai mk'nmnafysh': {%, m^mmedífheoreUaií)} 1 >41(11 .93(4,30?
Exsmple 40, A^4~llYdr«&amp;vphe«ylEN”{2-m&amp;íhoxYp¥rimldi«~5^dH.1”<ilmethYE§'46'' (f(3#?K’^***£íKv$'’3>4'd*hydmistKps8oll»»2CJÍ/)'yMc8rbíJ8v!)*tv3~ts«8*8di»x»!'4§''y!)"lif'' ¡> v reo k<E e a rho x a m Me iü'emeíiíai mkrmmuiyikí (%, mimsmed(theoreJkíd}} %C“68.07(68.45H%H-SCSa?)^»«í<iJ4(:l LOO)
Exatmtk iV»(4~I!yápoxy|íli««yl^I¿πΙΙβκΗού^ΑΜi «mett*yl«23^^$^ pyrrolopí3^||>yiidiw*S*y{)-5'<ÉÍ-||^S)-3«(«H>r|ih»Ílo~4-yÍí»«tliyl)''3>4“ ilI!iydr<>lsrM|«i^«5Íí»“2{lM)\vrk^r'b«3.níl|''l<,3-li«^«ftdtesftl-S-yl)“'ll#''pyrrelíí''3- pyrwfof2J^jp}riiÍin~$^}*$^fa{0$)*3»{»H>fphú&amp;H*4fyii»igiii$Í)-*3*4*tfi&amp;ydmteeqtámiÍil·'
Tnsr tille eonspeund ts obiamed in seeqidance of Ixaptpls I t»g |ke aeid oí Preparador* 11 ddl (Pk hsepinutíoo 3')tn Stop Λ, W ük eomponnd of Proparaiion \ I" in Stop C. Sím B¡ ?1{Μ^<Μ?%"ί^"$Μίζο<8βχβί5~χ&amp;Μφ}'η0^~3^·ΜΐΗ>χα^$$$$0$^Φ$&amp;ί!$!$$ Γο a ooh non oí 0 31 -i t.xC m«\n s oí -he <. οηχρΉηχΙ oí' Stop \ in 3 mL of acetíc ac«J Utero are addod 65 rng of xodinm eyanoitosohyílrsík 15 equivalente}. The seaetion mixtos o i« stirred for 3 boom at amhient íempeiature and thon ovemight ;d 50T/ The re are then udskb 3.6 cqrnxrdestts of sodium eyanohorohydñáe and the svaetiots mixturo is heated at XEO tcr ' boina I he npeinuno -o mp'.on\t bu t s-exosri suite rudditirm oi 2.6 eqoivakoUs ¡.0 sodtuits eyunoborohydnde and thest heating at 50°C íor 1 honra). Ah.tr coevapondimt <¡f the acede acid i?t the prexenec oí luhsenc. ihc residuo k saben np in 3 mí. oí snethanoh l ite pH oí the snlutiou is adj usted to 12 uaing IM rneshum.dk·. potasdiun hydroxide soiutmn. AJ'ter sdrring oxermghs at ombiestí lempos autK\ the toaetton mixture is poured mío saturated aqueots* xodumt bicarbonato soiutmn asid extrae sed xuds dkhioromubaoe. Ote rcxssidng or gante n.^e ts v okd ottb m-u** d>sed oxor X".?NOj din.sed and umeenoated ondes redneod preaxttro. The product oblahted ts porilkd by ovos ai lien ge!
Ipphlorontesltane/cíhanokammostk h4dvU6} and tlseáAn^híiaiJMtólÉep RPHs column (waíer/aceíoisttriie/tiiíluoroaeotic addx Altor ev aportólos» of the acetouitrile, the predneíi ix neutrniised uíth rodsusn bicarbonato h i a thou extractad whh otbyl acetato Ose urgame rhaee te dned ouu NaAd, hite red and eomoutmud nodos nxkeed presumo Ibe tostduc O&amp;t&amp;iaéd' m diMéív&amp;á i»· .a©et0.«iití}«': and q»a verted Into salí form with IM sí|«eo«s HC’I pipila, i he tilla .pmduct ts ohhbned in the íorm oía snifíi 3/igh-resoiííttim mms spe<itf>meiry (ESE) :
Bmphicai formnfo; (luM^TTOfe | Iv! =·Η eakulafod: 741.3395 [MAi}' nteasurcd: 74)3400
ImmuIiA. d:-l4"l!ydrax>:|daaívi|-d'd'2-0Uifla^^y¡syr.a.rddia':S~¥l}-!,2Hllmetbyl-5''!a~ ||(3d)»34míír|djaIb^4"y!midÍ\vi)~3,4"dil5yilraÍSi}qtyiMd¡R'd{!i7}»jdk’arbo^yi^IJ' lumrmifoxúBs~y 1)-117«pyr reí e-l-esrbexamiífo fmlrncyamte
Wgh -¡WMiafwn mms speiinmumy tESH};
Empírica I formula: C^I-U>Ní(ü·? j'3IHIi' calcuiated: 73 7005! Ϊ Μ·'· Η Γ mensurad: 7 I 7.3031 5L (referencc examplm S353j{Ba^fo,karf>auu.>yí)aa0au|mrtkyl|-.3-|¡{3á5P3'Uu3!pv1-3>4folEydraítí(a|ulaall«·'
IC1 i?jhylIc«rb»ay'l|^ftí^»yi)-Af»<4-hydr<Mcfplb«8yi)-/Ví I ^Hraeifeyl· I mrtmxmniíh
The tale compound is uhiained in aecurdance with the procesa oíExampk 40 using btmxyj Isocya.nate as aeyladag agení in Step 0.
Eiemetffm* m&amp;tüxnúiysh:' {%> memm'edfíkeoreÍkal}}
Mmm spPéfápmBry (Mífp Empírica) formiüar CTí4^M5©4 | Μ ?T 11: eaíaufatód: 030,3131 i Ti 0ΓΓ nanusured: 656.3220
Isaniikll, I relercísce exutnple) ^'f{^Íl(^íttyl€srbamoyl)aí»m«)methyÍ}"2-f|{3lfV^-m®th3ík1t*MÍIIi^4rols»í|uli«íllii?i 2(lí/>-ylf£»rÍm«yiJphcuyÍH^4-hytlrax>’phe«yÍ)bVJ^íHmeibyÍ-I##-pyrr»Sc!~3'í cárbaxamkft i'he tille cnmpouttd is obnümM masermteep wltb the posesa oí Bxapple 40 uppg ethyi isoevanatc as Mytetlpg age in Step C, /20mmtfrti mierwmaiysis; {%, memuteiíífhenretk'ai}} %(>?! .4(?02};%1 i~6.41|6.62);HN“9 i 24(11 .8) Π{φ -resm'tkkm mm$ speetrom&amp;iry (ESir}·:
Hmpirleal funriula: C*$l IMsk’k :[Μ2!·1)'' e2sula.Uk!; S94J0S2: [M í H]: roeasurstí 59420(4)
EssiuíOs 53. (eofo'ence exaínpkO 2;-4421yily«xY|>ÍH^iyl)'-2512.4rl0u4lp4M22'|]{ll()M-melÍ5ylMa^9s9y4r«míH|^lnoll«'-· 2{Ií#)-'y¡|carfeímyl|»5''{}(pheiiosyaie«íyi)amino|metfeyl|pheayi)'*I/#-pyrroÍü^3«
The tillo sompound ss obislnsd in aesordanee wiíh tho proco as oí kxampk 40 usías 2-phenoxyacoíyl duoride as ¿cvlaling agen! in Spp i'. kiememm' mh’rMnejyus: fV ffieásnredftheorntivtíi}} 14C'-=7222(13 ;Γ5:κ%ίΙ:::Ι^2| (0/14)59^^-729:(11.5¾) Hígh-resoiufhm meas specfrwaefr}· (.(2572,' llmpIrlMÍ formula: C2syl sCI5 ¡ fvt ?! I] sfocuiymd: 0572019 IMd'll)" msásured: 031,3105
Id ampie 54< ímlVmmm exampka Mí4~Nyár0xy |sbc*i>1H ,a-diiH«ihyi*S^- l«3»4<-<l%^^pl^IÍ9- S{ tl/Pylf car ipmy i| 'k''0(plwppxj's«^íyÍ>8p:PiP>|pie5feyl}p!tgnyü'-M(l »m«*S$yM #£< pyrrpkPphéi py ridiü-S-ylH l/~|*yrr&amp;te»3»«&amp;rfe<>x&amp;nfiík
Eimietff&amp;i micrtmmuiya's; (%< mee$iín*(f{t&amp;eorefieai}} %Ο--73,87(Β.04);%Η-5.4?(5/;4);%Ν“ 10.26( I0J?> íívíorcncccxanipitíi· 5''(S'P.'||!2-|4-Fhsprpph^pyI|«?hyIkiil|>li<p^yl|spn«p)«tkyÍf-2''||{3.á,}'-3''0HHkyk144·' dshysl roism|umof m~'2{ I lí)*yí)«arb«»y llpheftyiH^.^hydroxyphenyiHVs I jl'trímethyk I /#- py m> I e--3 - t a r h e s &amp;mkk
Thy tille eóippoúrsd IpohtpmM ift mamámcz wBh Iké proeMs oí Bk ampie 4S yahig 2-{4» íluo?ophenyl)eü«u*esuíjsbony) ehiodde as acylaímg agem in Step D.
Bteme&amp;f&amp;f mietommivih': {%t memuredfíheoreikaf)}
liiyh-resotuíion mms xpirctmmrtry (E8l·*·}; ír'mpifica! formula: ( .¡¡Mj J'NPOsS jMHfj* ededamd. 72 MUI 8 Ilvk-Bf measu red:: 72330 i I
Id ampie SL· (yelerence expropie* 545-|2'-|{Be«xyiearinpp(>yÍ}aíPÍap|elhyÍ|''2d|P^}^"»H-Ayl-'3,4"d5lwd?'Pisoí|pii}«ls!i-' 2Π l/)»,yi|cíirl>oay Í}phenylí"A,''-<4-byár»xypheiiy!)“Aí, Idklrkmkhykl 2l~py mde~3» mrb«myskk! i he Hile emnpmmd h ohimned io íUmordmum wüh 5he process oí ídasnpk: 45 u.sioy hen/.U isoevaruhe as aeylMotg appl in Sfep fe
Eiemeníiii mivrammiiysh; (%, memn?t>d{fhmrt>ÍieaÍ}} m&amp;mÁ4(nj2)^p^Míñ.mfm^4MWÁ€}
Htgh-res&amp;hw'im mms specírometry {ESI*}:
Empirieal formula; |M-35f cafcuíaieü: 670.336$ |M*HJ* müt&amp;ueed: 670.33%
Esample 5?. (relesena: example; íY''<4*l'íydru>í>plií:m''i^/V>lí2«írimethyl»S-{2“||E3i?}»3»íneíhy!«3t4»dlfe5?drélsííqi«fieÍ¡íí“ 2fl:ll)''>d|€urbí>uyl|"5''|2"|{|>l.«;ayl«cetyl}uíul^u|eihyl|pbeñ>>l^l#l-'|i>!rmte»3'' cíuixíMomkí
Tha Otle mmfütíná is oMninsd i» .«emMaaee svfth t.he pwe of ixampb 45 uslng eMpríde as acyiatmg agcnt in Step Π.
Eiememai mieroiinah-ik; {%< meastmdfíheoreiíeuO) %C-75.46(75.21 };%H-6.!U6.46);%N=^,31 (8.56) 'HigfafMeMim m&amp;m -Mpectrúmefry (ESfl·} <
Empírica! íonmika (\s;Hs?N63.4 |'Μ·Η Π' calculated: 655.32X6 (Μ*) U' meosufed: 655.3285 ICxaíBiiie Sjl» (rs/íanasce ^xarople) A6(4“lly<lra^yplses5yl|“A:il;24Haisll5yl''S~f2"(|f3^K2“«K4byl»3,4''dlbyilr<>lsoí.|umaiu^ 2(1//}^4|ί8ΐ4Η}^1}*5^2^ξϊ^ηνΕ8Γΐ«^ίανΙ}&amp;^Μΐ»)££^Ι| piie&amp;ytH^pyíTahKE-esrhoxíiímdfc
The tifie comp*>uad 6; obtameü in ¿it-cordunee with üie proee&amp;s ot'Txample 45 using pheml ísucyanate as acyiaíing agent in 5t$?p D. 67emenfai mh'rmttiitiysfo: {%, mea*u?¿d{íhe#?i?fia}i$)} %€: !!!?i JS(7 3.26} ;%!$-6.i2{6Jj;%N -f 31 (10.68)
High~re$t>iufhm ntms speetr&amp;fftMtf flSffihlí Empírica! .formula: mmr Micutatóá; 666,2230 !' sjeasureE: 6:16,3226
IkMSllkJli (retcrence sxampk> s 5,.|4„|({|2,(4,,irp,?arí>|PH?nylHclcv!|rr6p6racyllaaEcc)meth¥l|";2'-11(3^63-^^66^63,4» dlhyc!ro*s«í|«moil»'"2< .1 lí)yy!|earlnmyi| pheoylTAMÍ 4"!.cv6rc^y|6irrcyi)~A2! ,2'CrinKChyT ! í:l~py sr rcteT^&amp;riKsxsmkte
The lUití eumpoum! 1¾ oblaino! m accordanee wiih ihe procesa nf Kxao*píe 33 ucipg 2-14-flpprpiíhenyl}eihímesulpbony} chlortde as sulphonyiaiiun agen? m Step O,
Elemental mietomnirsis: (%,memmed&amp;he&amp;reimil}} %H-5J9{5^3í;%N«7/2S{7.9);S«4.45{4.52}:%C-6C>.!?C67.?g} iíigk·· remim'hn mass specfr&amp;meít}’ (TETE;
Knipíricai ionmüa: í\u2 EThíTpS.
[Mi ΠΓ cak'uWiíed: 706,2366 [MUI[' meaeured: ?Q0 7366 E sámele él!, 6'3S42iy6sx?^2''fl{3á>vT|f2^6h«clhylami«6)cífepiy|ipeihy!|-'3,4« dlbyílrofeeqtti»olla>2{ti#)-yl|€arbonyI)ph«8yl>*A^<4*hyílrox3f|íNiftyl)~B2”^:Í|ftfífcflM^· phe«yÍ-1//-pyrroÍe~3-earh«xam5<Jí; bydracbforíde
The pmeess oí l'-.xampie 2.6 is nsod, un thc one lumd repiacina ihc *M2·· brornoethy i )m«rph<>}iae hydrobromaie nsed m Step B hy ITehlnroeüvvfdinnnhylarniae bydroehlorhle and m* tile oíter haná ádding. a. eaíalyito. ammint oí tütjrahutyiantmonittm iodlde, ííigh-ntwiuíftm mus’í ípecmmn^n· {£$$+}: hnspiraah lomuda: Γ^Η^ΠΚ^ 6 P'H3f MéSiíatesk 617,2390 measmed: 677,2337 k 1L ΛΑ44* IIydroxyp&amp;e«yÍ)*1 32-dtpMkbyLS-Cb- | |(Λ^Ι*ίϋι#Μ^^4« 1 Isjc'cl rs.ílsí.>^ í.s II sí - J:í .1 ##1-¾1 ^ | c 5¾ rlí-t.ssjv 11" 1 <3" l>eíj*ís<l scí xíí ^"S-y il-íV-'fpy r ϊ íl ssí -4"y I 1 py rrokbk ea rb« .¾¾ m kla hy droeb I orkk Bígh-resofufim m¿m $pecmmtefn· {&amp;$$*};
Empírica! IbnnuUi: 05dfe%% | M ? I; | í.'íik'i.slated: 60! ,2445 [ M H s \' rmísaured: 60 i 2424
Es ampie 62< 5*(S-Fl«ofo-4*meíhosy“2^{{3$)“3“meíhyI'\3,4'4Hhydreis«qmm>lin'4!{Í//ih yl|e8rbo«yÍ)phe«yl}-iV-44*fey*lros>'fhe»yI)«!dkdimfethykAí«(!*meíbyM//"pyr&amp;*oM- yl)-l#/'pyrr<.ík''3'C^rbfiXí.ís>ikÍe
Elemental mlcraanafysist i%< nutasufedftheúretict»®) \ .35( 11,52>
High-res'ohaimt mass specímme??)’
Empines] ktvroukt'. C ísHí.sÍ N 4.L jXMM* cuicukucd; 608.2668 }M11| nKNtóured: <i08.2640
Esampie 63, 5-{ 5 -€: hlora-2-(1 (41?}~3«m«iby 1-3,4-bíby*trokmpíi*#im-2(14(}-yl|-€»rbi«iyÍ|i>b«5yyl(-Ar-(4-hydTOxy|>h«iyy1|- L2-di»H3byÍ-Af-(l-Metl>ykIÍí-¡yyrrol«|2J''b|" pyr(ibí^A-yl>'!.IAp;vrri4i:"3-i;arl.H>^a«k<!i; ítfemmfai mkmmuifysk: {%, tmmuml{tí¡eoreikal}} %C-?0,48|70JS(5»A.6?(5,32);%N-! LA ÍO 87)
Higkre.mlmmn mms sp&amp;cmmtaiif Bnypkk&amp;í formula: |ΜΗ!Γ caicukíed: 666 2242 iNt + H|’ OKa-aecd, om<,335'· ISMl&amp;tátJdi Evloroneo cxampte) MU- Tri ms i tí y 1-66( 1 -mv t h y Μ77' py rra lo 12,3-á] py ríd I &amp; "5-y l)-S-|é - {f l?«^rpliollr^4”yloietbyl}^,4''Clllyy?h'0^opiúíHíy?S''2(ll2}"yl|s.'g.rlKsayl!~l>3'^^wáte®t'' 5"yl}" I H-pyn'«k~.V€»rfeos‘í*«3i*le hytlroohSoriáe i-iigh~?ewhit!0n mas\ \pecfronmí'y (EStij;
Emgme&amp;j. fgmnitai CyHgaN&amp;Os |Ivfi I!I caícuhted; 66 Π i 33 |6f i Hf mpósimdi:: 66! ,3: 123
Exampio 65, lt2-l>lmetEyEA?~(!'-íni:ilsyl'Í2l~pyo'«te|23~6|pymllft"5~yl)~S"l6"||{36')“6“ |saorpholís-4-ylm&amp;tlpdK^3Hlltiy<lrofeoqoÍ5j0lkí''2(ll:/pyl¡e^rbimyl)''l3''l>o«^HlÉ>ypl'· S-ylhlv-pho^yl'' 176py rroloEWarbíMaeikk hyáro«fe!«réík
Migft-resefitíim mexs specimmeíty (Ε$Ι*β;
Empírica! ksnspls; .¾¾¾¾ ΙΜϊΠΓ calculaba: 723.33116 jM4!f myaMftó 723,3217
Esampte 66, {rderence exampie} 4~Melhylpbeny! {4~|4~{í44miraxyph*my}km£ÍhylkmrbamoyÍI~í,SHMme*hyM7/<· pyrrol-2-vl}-3“{{{3E)-3l-mí!íl}yí-'3»4-í'^kydroN«q«íaolm“2{lil}''y?|- osi r b un y 1} bemty 1 U’urh sí m a I o
The hilo eompsmnd E obhííned m acoomance wsíb ihe procos oí kxampk·. 32 usina 4-metby i ptesyl éhl omformaíe as· my I aifeg agón i i» Step; II. iiigh~resuiutk)i'i mms specfrometfy (ESIEk Em pi rtc a I formal a: B.$ |Mi III: eaícuiated; 657.307*3 saoasomá:: 65 7,3 676 2|Íl?)"yl)cs^fcÉpiI||?|ief^!>^44-hyir^phenylhIt2-ilwlllffc#-CI“pielfe|íl^l|l:*- pyra««ib4»yÍpl¿^pyrr»le^'€arb«x«mtd£hy$r»ehloriíi§
Efemenfaí mkrmumfysm {%> memmed{íhim’?mai}} r ^ í>*»,6> js>.' n 5 *&amp;> vΛ n isj; o;>ví λί~ * **(sam
Enmate 68, 64CS#lM»ro«l^#8sp^f^ flp I H»pyrmh¡~fritéthq$uim I<l« ,A H\ Nokiíion of iribromoivrane t i J upúvaloné) in dieídoromeihasu» b «««red rap^iK, ai •imbuid M# ;S.-spl.gildn of fc edmfpdod of Psampie 62 m didiled^ttee,
Tfedpdmtiod m repeled t^lc6 Id «jder W ddmpt^ tfeg JmcUoft in 5 faours. Tito mustím mixture i» poured ioto aoli> d^ous .ef&amp;md ai P£> Añ^mk^ fm llftmute, saturatcd iKpuxms Nal-ICO* xolutíon * addod. Ebé tóalas® p sxtmoted wüfe #ol$MBmettaQe. dried «per N&amp;38O4 pd ei«ed«tmíP lo dtyñbpi H»' p«duct obtamed ts podido! by ,pv^m$m ifftt mm w®M* TFA andmm<^ek as eluant$‘ Tlw· P11 of lhe app^pnafc Sdte m adjusted lo 12 with seíajuted »3»««* Na!íCG3 sohuiom and íhen the aeeiooUrdc ís evap^^ate^i^.u^^j^dciá'.-píe^11^· *^e aqooous resumo h extracted wlib diehioromothanei :1¾¾. «fgasle filase p; dbp<! :N%S0| ma evaporated tu dryaes#: if yieíddSiodlíie «deifpüpd.
Higk-resúimion mms spixínmnxny (L'Sí'):
Ldnpiríeai formula. C. J-P J-Nd p (M í \ \ f adcuíakídí 594.2511 |M -Mlp mo&amp;preéb .|f 4.251 ?:
Exornóle 69. m4-Uyúrmyphmyma^^^^^i%^^mm^^tmMA" yl>«setfs> 11 ~3 »*l ~·<ϊ i Ity <S rs>iso<|is Ss>#.* i i íi-3E ^ 1 |/^y||€dFb®^y^Pl3“^^®^di«xoP5vy|p.Pd'l*.' !%ή -rewhttíon mms spectrometrp {£?§$*}<
Bropimaí formula; C«H4sN?0$ [MRÍJ· cateulated: 7S2 5555 |MHlf measursd; 752.3552
Esample 7(8 77-(4 *Hydtmy pbeayi)-!v2'dl«ieibyÍ"5~Cé~| ylJmsihylJ-S^HlIhydroIsfcqumelin-^tiTkylfcsrbonyll-l^-beas&amp;dSuxaS-S-yiHV-(|>y ndfo* 4 -y i}«jH- |>y rro le-3 r boxa mhl e i* y ú raeh i ande
Hi»h~rt:%oluf¿on mws¡ ipecfrpmMry l'.mpmcai formula: Ο^ΙΙ^Ν^Ο? [Μ : H)' calcete!; »3289 |Μ··ΗΓ :hr«:<urcd: <>99 3293
Bsampit? ?L •:S«(5-Cbhr«*^~(|(M>3-<m«t|lfee!Í3*«l¿fteétli>i}-3,4'»íllhydr«isft(|«l8»ll«ii'. 2{li?}'yl)c'arb««yiiphe^yl}''Aí-24'4y¥dros.y|>liepyl}-I,2-dimi4hykk;»fl~pisilsyl~'ll7~ i;wm>¡of 1,3-é)py rkimS~yI}-172"pyrroiικΊ-carfo>s.»m k1e hydraeMíiride iiigh-resaíufson mass, \'pecín>meír\' (f'S.l··}:
Empírica! fonnula: OiOUO ίΝ,Λλ; [MEfff Cdeaiated: 223.3289 |M i 1! | r M-uu-ed: 72.23287
Baumspfo 72, 5·23-<Γ.:!ΕοΓο-2'3Ι82^)'·3·|ίΡΐίΓ|2ριΗρ^''>3ηιι92νΙ}<·3«4-252}'ί.ΐΓΡΕΡί|αΐΡοΜΡ'· 2(12í}'>Í|iarlK!!.yvi|phetíyl}'p3M4'2yydmtyp!ieayl>d^^'dk«i4h¥l'A!3d'nHdii>i'll/· be m. \ muhm-i i -S-y l|-17f*py r ¡ra le* 3 - ea rb»MmM«
Eiemeiítai micrametesh'? {%, twmttmÍftheúretkai}} %C-88 J9(69.1 .40; \ 1.52}
Esample 712 &amp;^4-£lydsmypheny!H ,2-dimeih>4»5-{6“(f(M|*3»(morphoU«-4*· ylíaefhyl)''3í4-dífeyílroisííqi«»oíi«*2{yp|)*y||esrÍMi«yl|-l3v'í-H»8*«dioxol''5"ylí*í¥“ {pyridiaO'-yl}·' 177 - py r γρ k-3 ·<' a r h« sus m kt δ íiihydroehforide mms specfr&amp;metry {£$1*};
EmpMMl $émmM Cm [M-'-Hf' cateidiáed: 686L2§73: [Ma-l'Il' iMaíliM'SíI- ES6.:2§7i
Enmate 74, <Μ$-α*!θΓθ.2- OI35>3'a'mor|>h<>iMi»4-ykm^fej'lE344HliHydmiso^islíM?ÍÉi-20//H31c^rb«m1|pls«ijyl}-iV''{4~kydn>syphj.®yi)-'l,2^Ikii'elhykV'|pyrykV'4-yl)-llf-py rrak--d-earbasam kl a hydroehloríde Hígh~?eu>h¿fkm mmv ,ψecírmnet'ry {ESh};
Empírica i fondula: <' v¡H ^C1 N*0* !M+H]: cakuiaicd: 2685 iEivHf measured: 676.2684
Examsitc TS, /Vp4-11 y <1myypbe6yf6!"m«iiwE/V-4 f..m<>$j*ypf |Epy M~y()~S4E- !^3Sl~3~(míU'plK4k^4~ylmekiy1)-4pÍ'-ks¡rydr?3?síMpíb'5«!i?^~2Cl llpylIcarhojyylhiíB'' lKaw^>di»xoES'y'i|-'2-4lráiluoraaH4lyyÍ|'lll''pyrrale-3''Carb«aamié8 hydr»dit»ride
Efementai mhromuily.ih: f% íheoreñmtmemun'il} %C>60.! 2:59.77: %\ 0«4,92:4.76: %N~ 10.75b 10.39 %C1:^4JS;5J 7
High-remfciífoft mass $peÍ'in>metry (ES i*·);
Epipkie&amp;l 'fppinila; (.7¾ lis? E; H;, (9. jMHÍj:'caUu»faicd: 743.2799 [M-' \ Í’J" meeaured: 742.280.2
Exaunpte 76, 24l)íH«»rííi»eífeyl)-Aí“(4'hydrftxyphe»vi)-1 -mctliyEA^ 1 -meíhyk 1 M~ pyra#.ol~4*yÍ}*5“Cb-(|{3á'}“3*<í»orphftlm«l-yÍmí;íhyí>»3}4'*clll>yclrob««|iii»oím*l<Si:/)* ylfcsrbonyO-Isx^henxoíliaxai-S-viVH/'pyrnde-sVcarbiesaínlds iMiiiti.m /V-(4'4Iydrísxypltsii¥Í}'2-{mfcih&amp;itymefhyl}-Í -methyl-/V-| 1-methyS» I ^ pyrs^i-4-yl}'-5-Cé"|¡CM>3~|mí>rpi^>lHV'4''ylmeíI>yÍ}-3s4*slfcHlrois«<|iti^í'sÍÍs»2(!#l)-ylf a*rfR«iyl) -134Kniyodln:aik5~ylH !;/-¡>y r rele-3 -i' art.Kix swa Id e íixampk 78, 2-(3>3~l>?f1^«i'iK;1hy|)-'jV-(4-hy^n>^ypfeimyl)''l''metlyyÍ-Aí-|!-i1iefhyl-II/-pyraEfíl'*4-yl)''S'-í6-||{3iiS5'»l-(morph«í?8~4-yfí»í;lliyl}''3í4-<lihyíír»is»q«líiolp<'|.(Í.#)^: y I ¡ ai rÍH.snv 11-13--¾ eModksed-S-y i}--1!!-- py r rofe-3 -e&amp;Apya sti ide
Foa^t&amp;le Λ~{4" 11 vilrex yphe nyI)- l-sneíhyl»¡V-41 -ineíhy 1-1 /l-pyrax»Í-4-yl)-5-(é- ||(3Sl'3-<iii«rpliídli^4--yÍm€lhylKVlH'liiiyiir«ií>i>i|iiiii<i11íi''2Clil#H^H-^l>íW^"!^v' haMod ios o 1-5-y 1} - 242 * 13~t n 11 u « r octhy 1)-1 l.í- p y rre k-3 -r arho $ 8m kk
Kmmftte.M., /V-<4~Hydr«xyph«»yl>'^-<2-metíjexyeihyl)-Í'«mtlliyl-'/V-Cl~métliyMH-« pysm0M-yl)-5-{6-(f{3áy33'morphoy8-4-ylmethyl|-344-dihyxh*»is«q«i*i»lm-2{Í/i}-yl}airhü»yl) -1
Esampk 81, 3--(4 - í I y dreyypheiiv 1)- 1 -ii*ethyl-$~{íMfl3$)~3~m«(ln‘Í~S,4~ dihydffíisoc|ttíii»l*«-2{ Ui)-y l{esrh»»yl) -13“h«»*«*1k£íi¡-5-yIHV-( 1 -inethy t-1 f.í~ pyr8£oM«yl)-£”f2^»í»*^halia-4»yl>€ihyl|<*Íi/-pyrroie-3-a*rb&amp;s;&amp;mid« kydreektprfdg fhe procedare is in accordante wuh the general pmtocol oí Exampie 1 um«£ (he componed of Preparaiiou 22 in Siep Λ. fhe proüuct obtuined is ttnaüy disolved in aodonitnkr and conceded Mo salí tono usdog C1.1.M aqueaus MCI soJiiíiae. Áftzr a lypphüiaaiion Oep.. the exponed componed is obnsined in the fono ol e salid.
Higk-waohtfhm mass spei-ímmeíry (/257-0.-Empírica! formula: C.«> hl.c NO fk \Μ ; 1 i Γ enlcuhíed: 703.3239 jM '-nf meaxaerJ; 7(0.3216 I he procedan? ?s m ¿ecordmive \%kh ihe general proíoeol oí Fsamplu 1 wmg ihe euMpuimá oí PrefMi;aikm 23 m Siep A.
High-rrsofitfmn mms %pec.fmmetry {ESI*}?
Fmpincai formula; C<vj Rso N6 í.k [MΉ 1 j' eolcnhued: <:AU 30S.2 fMHTf meaaurad; 68MJ0K5
<(·!< 2-(liíflusír^fttelhyií-A^C^Iiyilroxypfeeayli-I^iaaetliyl-iV-CI l>5'rrulo!2,3kkp>0'idiu-S-yl)-S-(t^djl3S)'3dd«arp!mÍio-4-yÍmtlhylp-3t4-íiíhykroim|iouolio~2(t í#)~¥lKwímmd|R3RKW.od$ox'íd-5~>kH^~pyna3ki~.$- c&amp;rhoxamkl* η2τΑ^ΜΜ>ϊίατ^ΑηΜϊί> '^' V i ^ ^ f^ Í> ^ 3' ^ ^ ^ ^ ^ ^ dihyd!xrkoí|rduodoRlllí)'ylkaarÍai:U):l|''l ?3d>í-u^j:Hllosid"S~y()4fEpy?oake'3~ aarlmxaotkk
.EadtMi3l%.$.?> AM4»Hydroxy pfeeay t)»l-otetby yv^ I S"yl|-SdíHf^^'M^orPboÍi3*"4»yim«lhyÍ)'3*4»dihydr»lxm§«m8lii*'4l{Í£f)* yÍ}carfc»ayl|~Ií3~b«n*oilÍQxol-'S~yl)^’*f2t2í2'4rill»#r»elfeyl)-ll#^yrr«íe»3<«»rbex»mi4«
Ex&amp;mpSe 88, AM4«lly<ly»^ypln6ayl)«-2«(2*»i«Ü»oxyetliyÍ>-픫iethyl-AMI-a|#^yM#'“' l>):rrok>|3,3"l>! |yerkHo-S-yird-|é -Ί |(3>S)-3'<morplíftti»«4~y <1ihydroisíW|BÍmsl«í'2fl#í}~yH«arb0!íyl}"13-bi'«£«dio^^ík3''ylpliíEpym)Ie«3''‘ ca xhn m mide Isy droe h lo si de
The proceduR* te in accorflaitco vviih ?he geoeraí prolocof «f hxample I uaiop tfee appfopdai© Rr8paratí««s¿ The prodoid otdamed &amp; frnaUy disolved i a <aceH>nüfüt‘ a:ad •amvened ímo xah f»rm usiog 0.1H aq»euu.s HC1 soiuhom Afier a lyophHi^itiot» atep, she expee i ed eomponnd h ohubned lo tbe ÍOrni oí(¡ soiíd.
EiemexÉai mícmmml?.ms: (%f íh&amp;orefk'ai;mem'nred}
M€-6$.b?:b6 I5| 968^78^.4^ 03:0:10,24: 053(3=333:4 JO fíigh'+esofutítm man spacirameír}f-(áWi&amp;%áJW^'M^:M0^W)*
Empmcal formula: C**1¼ li$ O? IM'i H.r cfoeukded; 783.3501 iMfoif measured'. /88 3502
Exasuple 89. /V*(4-llydimyphenyl)4»meíhyl”AMI~i«eíhyl»l/l,"pyrríib|23”élpyridifi-5'pi|'4~|2-{soorplo'.0io-4'yi)ed.s)iÍ'\3-(€~][(3il)-3'Oosdi>yÍ'\3í4“dihydroisfH|a0i:oliO''2(llí)'' yl}carliO«yIí-lí3*heííx<ídíoxí>í-5''.yl)“llf“pyrs'í>le''3*€arh»xamÍde
Eiapspte 0(1, .&amp;34-Hydrosyphe«yÍ)4H»ieíhyÍ-4V~{lHme$hyÍ4í7~pym>tef23^|pyHdi&amp;- yÍ|ésy%p%d]'d3^Hmíodfeol''5'yl)'li:Epys'í'ok''-3'-e8rií<.>ii'amlde liMIltdklL A4'4-ílydjmy p £* eny I ΚΙ- snethy NV41 - metby E I #f~py rasseEd -y 1 )-5-( é~ i ¡ (3S') -3 ~C a rplidllh^yi soatlty 1)-3,43 ihy ñ rblM^siü^i I» »2( i.#l)-y!|es^ rboayli'i 3 -besi«s.o3l<>xol-S-yl)>ll;0^|wr.mk>3'>«arhoxoo5Íde llamáis^: i~ltlyry^ppleii^ hyilrMÍkml4
The proccdure is m aeconianee wíih ihe prcrtocol describeáüm Ilxámple % usb5i &amp;£ eompoíinús fn»n Pregas as 50 asa; V hs Mep V and the cxanpssusvl tVmn Prepamdmi 1” m Sh'í* 0 The produ v ^Haku 3 s* daaih iksaoíu'3 m aeetoudsdc asid c<>nves(es3 mto sált Jbrm us.ing OJ.M aqsasoua ΠΠ solsdíars, Aíh;r a hasphihsaíson step, ihs.· expee¡«<1 eompound sa ohUiint-d m the íbrm oía «oiid, (%< tkem't?fk'aí:memmeií} '* ,\ n"li4* *, 18*' * »' fíií>h-remhitíott mms %petimnteiri' íKM’glg Kmpineai fórmala: ( |\1 ' Hf eakaoased' 703,3239 |NI5 H]' mess-ased; 703.3236 Éismnfe %&amp; JV>44«ilyif rosy pfc*nyIH -<2-mefb»xyetóy1 ^líietltyM &amp;. pyr»K«M*> 1)-5-16- |fs'35KHmorpheliiv'4"yteetbyl)^3l!4"diljyilre¡ís«s|«ÍB0liíik3(l##h yijoí rhíi»y 11“ 1 ,34hw.íuI fí>&amp;e!»$~yl)~ 111- py rrs> hei-ma γΙμμ^ο lile lsydr«eldoride
Eiemenkti micmítnúfysisí (%, theoreíse^tme^mmí) Λ dt) oí i’ 4 i ^bííd. t\ *0 8n„*oU 4 o i *4/0 Hígh-tWfhiihm mms sprarometri· •fESIpb'
Empujad ν-τηυΐν IT.s V O |M-i Ml^aíeolaiedr 733.3344 |M--H f mOíHured: 733.3345 lita a malo94,- í~-i2~¥iu®f?mthyl)~N''{4~'hyúnixyphÍmyly2~m$t'hyl~N4l''mitfhyl"tH~ pymKíí!''4''yí)'S”(6«|fCM|*3“<mftrphoSí»*4-ylmetfeyl}‘3*4“dils>'dríiiso<iiisi»»lin''2{1?l)-yl|cs^rhñ35yl>-4v1'tieí^^«di«s»l~:S-vl>'I4l~pvrri4e-x4H^irl>o‘s.5s.?jílí.!s; hydrooh bride Use píoeeduru Is in aeeordanee vOtls tlse pro tocos dtacsihed sn Stops Α-Ι> oí l-xsssnpk: 1, rssbg íhe compnnnds íton? Preparaban?; ?J and X u\ Stop Λ asid be «,'ontpound írmst iPreparaüon ]” in Sun? C Tho product ohtained is nnaily disolved in acetonitrib asid ponvcncd imo salí (brm using CUM aqueous HCI $o!uiion> AÉer '$ΧΦ$* Éb oxpecsed compou&amp;d is ohtained in sise lorm oía solid. EMmeníM mmmutmtysmí f% ¿kmMtimfcmemureti) %ζ>ñM4MMl 3bbS. 50:5.09; %N- 11.10:1 1.02; %ί.3-4,0Κ:4.?4
High-nasoiatioti man' xpecirúmetry (ESli); \ :mpi rica! lomuda: ( 4.2 U· 1'N;A. |NI-»-1-11f oabubíed: 72ÍJN4 fKWIf mcusurcd; 721J14?
Ibdnmbffi I-p,24'1if1iolriH4h>4)-iV~l4"ipydmsfpl«yl)~2-'Oi;i?íl5yl-A^CI -methyf- I ii~ pyrjixisl»4pv1|'ñH6“i|0M,}%'l4.)i?arplusl0?''4'UÍi5sed^yl}%4,4'di0y<irakaq«03i:Ola'2flll>· yljiwbimyib^3~hon;mdbMd25~yÍ}~1//~pym'.deAcarÍ>o:ss»ti<b hyatreohbtide The ütlo i. empotrad is ohtained in accnrdanco ^uh tho procos» íd'Fxampb 1 using íhe acal td Propa radon 32 and Oh? compound oí Prepararon .'.?' in Step A. and íhe compound oí P reparado» i" in Stop C Too cowpound ohtained i» eonvortcü soto solí lorm &amp;aá tyoplbl bebas desebbtdin the-general procesa i© otóíbthb sitio compabmt.
EiemvMf(tí mii.míitH*{psis: {%> fh&amp;oreiícifitmemured) mem MMJ9; %H 5.33:5.04; %N-I0,54;t 0,15; %a-4.57:4.55
Wgk-n^ifiut'km mas* spectram&amp;ry (MSiX/BM, ESÍXIM):
Empírica! íormuia: <7Í<S Η.;;: ΙΑ A-, O.. |:\t*!ΙΓoalcíshnod: /37,305(5 mensurad: 737.3052 E\íRllk.£!b I !<3lé>-3-s»<^l>yi-3s4- 1 i 1 '.^ ^ ^ ^ Λ ^ "'' $}~l-&amp;morphúí$methyí}p}(rrúíe-J~C8rb&amp;x8mMe
Vho vonnMmai «dn-bucd m Siep H oí bxample dd L dsxsohed i» <> mí. onekahydrofuran. S.*dmm .Mide *¡oo soya do* mnnO ^ added. LOovu'd hs mofphobne *0.3 i mí. ? 53 mmoh Á¡^¡n\ Le 1 i a· í cacho» mixture Ls heated m a seakd llusk af 6d' (' íor 72 hours. Aííer eooling. thc sokmob il evapísraíed %® ;ρϊ|: ¡|f pi#Íi«| # piitM %’ dtrormrUau'apby over si Uca gi: asing dichloromd.hane and meth&amp;noí as aíuams. lie expected product is obíaincd m the ftwAitet,,. ¡II NMR (500 MI b. dmsmdó) I ppm: 7,7-7,45 (4 h&amp;¿ 1 1% 7.2--6.9 fm. 4 Hl, 7.1 -6.4 (m, 7 iík ?. i-6.¾ (4 hst 1 Η), 7-6.8 14 m> 2 H). 6.75/6.48 (d-tlst 2 Hg 6.Í (4 bsg 2 H)s 5.25-4.7 <4 b.v 1 !ík 5 2-3 8 tm. 2 ΙΟ. Α.Ψ'4 *33 8 0 m. I ÍÍX 3 75 {4 a, 3 ΪΠ, 05 (N.4 U), 3-2 (m. id m. 3 412 3 i2 b$, Uh. HhMLd o,78 {3 t*j, 3 LO. O 8b im. $ JI>. O i im, o \í) IR (ATR) caí! 1626 6 >0*0 amides 1 he píoeedure is ín aceirlanee wlh iís^líraroepi deserlbfd in^. ,O-iT;itS^«í^pÍ0:-3|->:-''íf>í>0: p rodad ohtamed is OnaHy subjeeíed ío a síep oí conversión dúo salí íorm i» i he presente oí HC! in eíher. Ader ídmaion and íyophilisation m a mixture oí acetonitrilcAvater. the expeéíed ébMpotmd is obubned. MígfcremMUH· mass spectíWmPf Epvpirieal ibrpnla: ido 1½¾ C% [Md-Mf eaicuiaied: 703 323¾ ÍMAilf measured: 7033331 dihydr«ísoqukmímdl{l l/}~¥Í)«^rlH>»vl| 43db««8odk&amp;<>k5'yÉ)~i ^2-(«mrpteliu<4~ y 1 )st hy! J-/¥«plnmyI-iII-pyrro le-3-enrOe-xasnUle hyilim.dibri.cle l he proceátire b m accord&amp;nce witli itie prolocol deacribed m b.xample % repiacing the compound of Preparación Γ by ihai oíPreparntion 2,:. mhwHítifiíyiist f*¿t fkt>(irefh'8$:mt>m&amp;red} %€-6B,6i ;<bd2; %H-íTO:S J3; V«N~7.62:?.5*É; %U-4J2:4.6ft
High-resohitiúti mms speciromeín (ESI/FIA/HR asid MS/MSF*
Kmpis'icai formula: C.121½ Rj Θ§ (Μ Hlf caíeu lated: 690.3 17? C M r 1Ί ]" ni eas u red; 69 9.3 i 7 3
Eaummk 98, Í-|2-(ilh.ueChylamiico}elbyl¡-AS'4-^ydroxypbemI|-2»mcdbyÍ''Sd6"||131)~ 3“í»etbyI“3,4*dlhydmbeqyi»»ll»4(ll7VySfearh«i*>d}~I3~fe$s^odia:mÍ-$yyí>~&amp;^h«»yb i//-pyrroÍíJ-3»c8rboxamid« byclrecblarkle
The pmcddMrd is hi aeeérdaheé Wlihihe protoeoi describid m Exampie 27 repiucmg $he compoimd of Prepararon !" hv chai oi Preparador* 373 Tha produce obiaioed is finaHy «subiected 5o a asen ol’ conversión hito salí fono in the presenee of 1M HC'I in ether. Álter ftUration and lyophihxabon m a mixture of ucetoruirí te*uuter. the oxpeeted rorapoond b obisined.
Efcmiffititf microattffíyste: {%, ihearedc<d:m('mi{red} MM] ¡filgh-re.mitdkm ttwss speckomedy (TSI/f l A/IiR and ÍV18/MS);
Enipirical formula: C&amp; !1SÍ; N.s Í O ] Ki e- H|' caienhitcd: 657.5U7Í jMdij' measured: 657.3066 tsydfs'Kíhkm<k'
Jfe 4* $ikyi }42~&amp;'η^Βκφ*8&amp;χΙϊ~3~Μΐ?*Ηχί·-!*~ί&amp;~ί0Β}~3~*η*ίΗ}>ϊ~3,4~(ί!ί>}'όϊν*ίΗ~ i\t^Uittofí^2-eefbm^~fJ‘hettZfldit*Xi^S»}4fpyrrt^^S^afbfíx^Íate
The prot-edure is sn aeeotdanet.' whb tíre psroioeoi deseríbed m Siep A oí Example I replacing Ose cotapcasad ol IVepsmOion 1 by fhai oí Preparadon 19. *H NMR (500 MI O.. dntscwttri í> pptm 7.35-6,95 (ra„ 9 Hh 7-9.,5 {.severa! s5 2 H), 9 35-5.55 {senrT s, l· v I ' f ew d , „ i \ ^ N v s ves 0 a - f!\ 494"^ *a s ,,κί 5 s« i IR 4.4 im. " H\ $ 3 <ro .' Hs, 3.50 6a. 2 U\ -V-jjs «savuai so, JIÜ ' '<-2 ; (severa! s< 3 ük 1,25-1.1 (sebera) sn, 3II). !,05-0,7 {se v oral d, 3 H)
Sien B; petó íhe pjoeedusvr ss os aceordanee wsth the protoeoi desenbed m Ssep H oí iwample I. 114 (ATI4) em'T 3000-2500 v -OH, 1675-1925 v -0-0 carhoxyhc aeíd -s- amide
Step Cr Í~(2~Bmryia\yeíh)^N^4~iU’tí~bulví(éimeíh}4}siM^Ayphm%iÍ~2~m^fhrí-S~Íé-
The:ptwelbPóI;ypceptóa9if ayfíhdpprprpeoli|essit|í>ed os Mep Coi ! xesapk 1 *11 NMR (500 MR*. Onsvo-dós ο ρρηο/. r- > ¡ovosas s, I IIκ T34-O.0 <„>evend ns, Π H-, M o 9 ssevesa! *5, 1 I Π, o 6 (severa! v 7 I!}. o 6 o * (as, ? IIP 6 05 sseur.si a, ? Ih, 5 2'; i " ssevesal s„ I Hs, 6 \ (> {¡>e\vsa! ssi, 4 !h. 4 54 4 o'* 75 (sauxal sn. 1 10 4,3 {m. ? II), 3 TM 3 (R ΐ s ,m. ' !H > *0-3 I (se v oí al so, 2 IR 2,4-2,29 iscvesa! s, 5 Hs, 1-0.72 (ss,o,';,4 ü, 3 ih, 0.9 sso'.ora! 9 Hs, 0 s s^eu'r.d s, 9 IH IR <ATR) esn'1: 1929 v >00 ansldee ϊ(3%^~8&amp;ί&amp;ρΚ*,4-άί^ί&amp;ϋ~1Η-&amp;ί*$»ίηαΙΪΜ'2~*ΊΜίΗ*ΐΐι,ψϊ.3-ίίί'ΜΖ(*(ΐΗίχ>>ί~$'ν$β’·\~(ϊ" in a hydrogenatmg reactor, (he eompotmd oí i he above Step is dissolved ín 30 mi. oí methanol, The soMíon is degassed by buhbhng argón ins and palladíam~o.rs-carbón 10 % (550 mes is added. The resulting suspensión is stirred tmder a presante oí i bar oí hydrogen fbr 1$ hoursgand thed pusaed ihtouglt a \¥haíman!-r hlmr. The eatalyst is rniaed' vvith methano; aml The fíltrale is evapórate*! in v&amp;dik The residuo thcreby ofnrdned is ipiiriled hpohmmatog?aphy over sUiea ge! «sing í he expee u?d poviiu 5 i" m4atned in (he tmm i*f an oí!. !U NMR {5U0 KiH/. py.ridino-d5} δ ppm: 7.7-7,4 (4 s, 1 H}, 7.)-6,9 í®, 4 Η K 7.1'6,8 (4 s, 1 ¡k, '-o,? ¡m, 3 1ÍK nn.í4 4 «m. 3 m,o,8-o4 (4 m, 3 ií> o.l (m. 3 U). \2·Λ4.7 (4 s, í H), 5,15-3.9 ¿g d, 2 11), 4,9-4.8 ím. 1 H), 4.9/4.7/3,85 O m, i M>. 3 9-3.7 (m, 2 H), 3,75 (2 s> 3 He 3 50.2 tm 3 11),3-2,4 60. ,) lh. 3.40 5 μ a< ' lis 1 050.95 0.75(4 0. 3>Ít,9,85(R 9 Η1 0.15-0(m, 6 13} m P&amp;P) cm'! :3346 v -OH primary aleohol, 1621 v 0>0 anudes h&amp;mttáwx0Í-$~}4fpyrmi*i*yÍfetk$ nmihmmndphmuiíe
The coropound oí the abovo Siep (2.37 g, 3.17 samo!) is dissolved io 10 pll oí ,'.ι 'loemhstas Im macum tr \n.< *s o tokd lo 1 < .notíut are vncefcSssveb ao<eü ,s ,UhOunsnu.< v! 8 ud ! > u mme ' and, thopRise, mthata solpboosl chloude (¡(46 od , S. 17 rmnuh. The reaeñon mixunc ss ava sed íor 2 hotos ,u Oí'.i he reaciien mixture o (hen poured irsto saioraidsi &amp;qnmm sodium hydrogen carbonate soluiiosamd extracmd 3 limes with ethyi acetare. The organic pitase is washed 3 times whh brine, dríed over MgSÜ.¡, ti Iterad and Oten evaposated to dryness. The evaporaüon residuo ís ased wiihoul poriiieoliort io thc «xa Step. '11 NMR ΛΟΟ ΜΜ , «. ο' .VI"---4* ^euaal * Μ, 2ι> b 4 ,ο\ Μ), ) ο '> ocxera; s. 1 IIX o 05·ΟΜ ser va al "'. 2 H3. ó.! (seseos .v 2 II). $.2-4.6 (seseral s, I i!>. 5.050.75 (mesera) d. 2 H). 4.9/4.6/3.9/08 (severai m, I íi). 4.3*4.05 (m, 2 HX 4.2*3.95 (ηχ 2 H). 3.75/3.? (2 s, 3 H), 3,05*2.5 Bes eral m5 2 :1¾3 .^&amp;$erai a, 3 !·!)» 2.45*2.3 {severa! % 3 HX !.05-0275 (severa! ¡i 3 Νχ O.fpveral s, O 1¾ 0,1 (severa; s, 6 H) m (ATR) ení!: 1620 v -Γ-0. 1340 v -SO* 1249 6 --0113, ! Π2 v -SO*
Stm F: N-J4~fteri~Bitt?t{iíum,íh}i}$tf)i}&amp;x$phetiy!j¡~i"f2~fó~methK\yt'fáyÍ{ffie&amp;}fmmÍmp* ep7/-2~?ra thi4~S~¡f'>~i{J$¿h3pth>Íhii'-L4~dÍh}P^i-4H4>>^£ihwÍhP>l>-<ú?iimí)!?"Í¿~ £K,»4ííí//'íí«#/-5~v//-A»f/~w«'//0>/-Í//*//>‘w;í3i/-4”r/i/íj<m>/*v3-ítf#-éíí.VfWí/íie
The compound nf the above Step U.33 mg, 1.61 mmoi) i$ UwsoKed m 6 mil ®fl
telrahydroíur&amp;ii. Kodium Indole i 10O mg. 0.67 truno!) is added, followed by dimeihyhnsifM ΐΟ.ΓΟΙ e. 1 6 0: mitra!) drnpwis;.. The foaetíon mhHav is healed Ln a seated liase; al oOH.' *v> Ό Kt era Ule* >*; olma, 4 '* e-rape aoJ \/> * '\,\ns <a i pmsiko e. -,brómame aphv >nei "lie.i o5 ; mí.y e >, X\>m".nnhare and amirra \ ahorne· e.mo! as olearas Vbe eepeeied product js ohialned in ihe t'uvm oía team, % HUI pil «fe {4 bs, 1 I IX 7.2-6.9 (m, 4 IIX 7.10.9 (4 &amp;> ! H), 7-6,7 14 m, 2 IlX 7/6.45 (2 m, 2 H), 6.8/6.45 (2 m, 2 H)s &amp;1 p, 2 1¾ $,22/5.05/4.7 (4 ha, 1 Ή), 5.2-3.8 Cm, 2 /1), 4.91/4,65/3.9 (3 m, 1 H>, 3.75 (2 bs, 3 II), 3.7 (m, 2 HX 3.2 (bs, 3 Ηχ 3-2 Im, 2 H), 2.7-2 pm o II), 2.7-2 {κχ 3 H), 2.45/2.35 (2 bs, 3 II), f .05/0.95/0.8 (3 I>4 3 ΙΙχ 0.9 p;í911)0).1 (m. Mil 11¾ (/ÍTH) cm*'"' 1628 (shouíder) 6 amides Síí-p (¿í N~(4~Hydtt)^ψitmyξH-P~¡^metha\y^hyI{methyi^mm(ψihyih2‘methyl·S^ '' he proeedure ts m arco i desee wiíh ihe protoeoS deeo ibed su htep l> oí Pxampk i. i he produei obiained O ímalk sohpvtcd m a ,Mep oí eosvecMon ínlo -adf íorm ¿a the prese ace o'' iM HOi m ellu; M)ei ítltiauan ard isophí.fs ιοοιι ia a ooM.sv ot 'KfHrut»' x\.tiet. fhe mpeeted eoovo‘s\t is «Natoed ΙΕ CETO: m'·í; 30ÜÓ to 5500 v -ΜΙΠΟΕ, 1615 y >00 anuden látT-'Ijéí δ >€-04: <, 141 v >€H-Ar ifigh'remiufkm mms speeíromeíry (£8f*); l'.nipifiOrnUiiíS! ·\.·δ\: h,;,n,o6 ΙΜ-ΗΓ eiucuhaed. 705.3395 !M ¡ ΗΓ measured: 705.3391
Examnle 14HK 545-'Ehnm>E'a-aeth<m;^1fE35V34ammf.0ud1a'd~ylnu4fn4E3oE' ddíydyi4.\CH|UHU>Ilíy5i07:7)'y!|carb<>«)3:{phy?íyl4AM4-hyíÍr«syphy?n'í)~!,2-dim«í4¥!yV~ |l»MyOsyd1:I7-pyraz<4-'4-yi)yil7»pyrm!e'3''y8r4osaml«le hydroefdorkie
The proeeüurc is «5 aceordaoce whh ihe genera! protoco! oí P.xampfe 1 using lite appropmíe Prepanulons. The produei ohuúned is ímally dissolved la acetonitrile and converted into sult iorm uslng 0.1 M scjneous HC.1 sohuion. Afíer a lyophslisanon step* the exnacaai eornpmmá o obudned in íhf.· Iorm ';í¡¡ salid.
Ekmumbd mh'ro&amp;nah'sis: {%, fíwtweik'iibmeasifted}
34C-04.23;64.31; %N-U .52:11.46; %€T-4 Jb:4JS
High-resoitkhm mms spei'innneíry Íl2b!+j; Ümpirical formula: (.:.,¾ Un Γ N« O-ÍMTH]* cakuiafcd: 693,5195 [El a|4|" meaxured: (4)5,3194
Ex ampie 16L 544'TdnmavS''nkthox¥"2'dfEM>NT4morpftoli.m4''¥laHdhy!EE4'· díhydredsot|olord5n“2(!M}»yl|éáyfemíy!||>fe«s(y1EAM4"hydr^yphenyll“lE~dlTO<dhtÍ# (l'Xnethyl' l íEpyra«dM*y!'Hhydrodiiaride
Ure litio eompouod Is ohlamed® aeeord&amp;rme wiílt (fe prpeess of likampie 1 isáñgihe ad# ai' Preparado?* 27 and (he eoroponnd of Prepararon 5' in Step Λ, and fhe eompound of
Ppptodfeíi Γ lp. Sbp €>· il'Kf eddípauáá dóiáiddá Is PbPVéMed Me sslt bmv má iyapldlksdiisfcse.rfcd: 4« th.e gsnsmt praysss b ©Pop thellie ppppsppdl fUemettt&amp;i mu'?(umüiy$í&amp;! 7% íhe&amp;redmí:tmasured} %C "64.23:63J2; %Fb 5.80:5.20; 53Ν-Π.522 i 38. %Cr-4.86:5b3
High-remittíhm ma\s s'peí-twmt&amp;fjy (ES'H/HR, ESÍ-/LR}:
Fmphkb formula- Γν. !L¡ F ^.Os |ΜΉ|* ,;2ou:a¡;:d· 643.095 IMOÍj' me a so red: (4)3 3!93 7smmp.b 102, 5 ~{S'C.l^i »r í>-2- f | < 21S) -2¾ - {C4- 1 p i 1 -y I>Jfií s ? fey l| «3 ^4~<11 l%y ti sí*- ísí»<|amotm~2{ !lí)“yi|«arbo*iyi} pise ay 1} €V»{4 - fsy dro xyjb «ay 1}» I *2~df meHsyI~Af~| 1 ~ aH4ls>i4#|3.pyrrc0í>!23'-0]pyrid6^b'4lF!3|3|pvrrsak''3H::ísrÍKs"s,amkk' tñlryúr«cbfemie Hígh-rewhttám mas.s .ym'frfanefr? (ESI' };
Bmpirícal iormula: €,5.211¾O N? Oí [MO.lf cafeubufe 742.326? [M OÍ f meaxured: 742.3268 £xgmgkJ03áS-(5-<331ihux^2.-f|<3S>3-fC4-mk4hylpi|M:rr^m~VyÍk$iikHh>i|<3#4--diby d rufeo© o ¡ m> 6 η »2( i 7:7)»ylf e&amp;rbm-ry !| ph«u y ife I *2»dim©$6yb/¥~{ 1 ~hí eíHy M 77» py rro te 12,3 -7* | py rícli a«$*y IHY» p he«y 1-1 //»py r roie«3«ca rbox» m kl«
IlllIMílitxilMi ^d4-llydroií.yph«$iy.i)·-1 ^-dimeihyl^V-f!-mstlwl-l ii-pynmíhíld^-éj''
pyndi»-'S'-yl)“5-{6-{|{3S)*3-(m«rphaii«“4*ylmfetliyí)'*3í4»illbydr«Ísoí|ttm«Sla-2{1i¥B yÍ|c»rb«»ylh^3-'S3«?«£oc!mobS»yl)”li^-pyrrole«3>carlioxsi*iicie
Isxam r»k 105,5d542fdara 3-»l ÍOSV3-4 me ro hcdfe»4·- v Imelhv I s»3.4»drb vd rima a «i mi li rr» 2( I /¿)'yi jearb<ni>· i) pheny lk Y~74~hydr»xyph««yl]h 1^dbmdbyi'dYd I -apfiyMjS'' díhy<ir»«i 77» py r r o lo \23 »6| py rid I a»5~yi)»177» py mík'-.WpiPS&amp;mkk ileillM. íHS-CMere·v ÍCWO-ylíceriJoayiÍP^eay^U^toeíKví'/VHI-mítfeyiaWiíjTMiep^lpy^^·5^1^ .V*f*hvnyM/fi*yr roK’-.l 'CarÍK^aísnie
The pmoedmv i'' ή', ooootdafico ujtb tho penes al pseioeai oí Examplc I d?99i : Λ appropssata Píepaudosss. 0 heüsg uiuk-nnntx! i huí ^fep i’) is sh>í ra,triad n«t. j -*e cspecloJ pmducí i\ ohoarec oí 9..<. ha.se ínnn. Eímtent&amp;i mknmmilysht {%, thcamiicaEmemured) %C-70.72:69.7?; %H-5.?9:5.96; %N-11.78:11.43 .0$gh*msi$i$^--ntan ipeefrmtetry (J£Si+)i Empírica! fonmda; CV- R<¡ Cl Ní. (.)< f.M *1!)'calculaíed; 713.3001 ¡|M+HJ* measured: 710.79911 py rro 1«! 23"&amp;] py ii di raS-y 1V- i i/~py m¡ le-3 *c&amp;rt$ asa?» Ola
Esnmplty 10S. S«(S>Oblor^^2«f|{3»!»>-3''CfStes^»faeiÍii«'o4«*ylmetÍi$rI>-3«4~^iHy^rdÍ|^.i^iiÍli^Íl^» 2í i 17}"yíj<airi>taíy1}|.>heriylpA:-'{5-'Cvaao-'I»í«i9hyO I //-|»y rrol-3-yí ííydmxyph€ftyl)'”l,2'“*lii»eíhyl’-l/í»pjrr»lfc''.Warfe«xamlíle hydroehlorMe ih\. Hle ^ampound \c ohtankd m no üat't o wlh ihe \> oaess offexample 1 usinp.Tke.acid sn 1‘ropas.sisón \ and f3.S>.'·¡.λ.snospiinlisa hnethv ‘ 1-1,3x3.4^etrahvdix>i^q.ttí«dilse·' fsee ra'ikuanor ίΛ ns Sun -\. and iba amnponnd oi Preparation !9!í ín Siep C. lile pisxluaf ohiainai is luudly suhjected to a síep of conversión inío salí, forra in ihe presencc ot 110* m esa*.*· '3 iter Oh misan and hnphdfmtum m a mknue oí nccinnunk* vmicp tho tvpeUcd composmd is obiaincd. (%, tkearéfi&amp;íEmewmreii} »U>64.95:65.0k %]1-5.45:5.30; %Ή-1136:11.26; 5461--4,79:4.02 ¡'mpn'ica¡ ñmmíu í'¡ M*>*'i 'X%M. fM-í-Hf caleced: 703.2704 1M H| mcavus^ü 'O'5 2 f'Xsmmfi? 1¾¾ $»{5*€l*toro*2l·· 1'ifIϊ>~3t4~«ír^is<K|»i^f>ii íiimelhyl’·I//«pyrr«le-.V*arfeoxamÍ4e bvílrocMorhis H¡c utíe < .-mpound h oMamod m ..KXVKtiUkv vuth ihe ptoois'x X í'wnplc i ncrng iho acid Pí PrepanMón ! and ihe compuund of Prepamttou Ύ m Stop A, and ihe compound oí 23” £&amp;. Si«p 12 Mnipauud oMp?sd la coef^fed Inte salí ten and; lyophí íised as dcsaribed in Ote generas proceas lo obíam Ote titile compensad,
High-resáftitítm mms specjrmmtry íf&amp;, &amp;$Í*/FM)·?
hmpírk'ai lomuda. (V 1 í s - * iΜ^Η,Γ calculad 70!.263S j\t \ * HuK'W *0i 110. S~{5-í.ldi5n:x^2-t|l33V3^('kK>rphnlp5*4-yLni<Pliyl)»3;(4"dtPyPrs:5Ís<K|i.da€(lia” 2(IM>yl|aar!H>kylípbanTl)''A!3,4''pyaanp:xxdín-2''yi>';\Xd~liydrakypl«xwÍ)''!,'2- |ydrp8|fer|i« ÍUemenmi mkmmmtym; (%,.ihmrefimktmemred) %OPS. 13X3.72; %} 1===-539:4.76; %NM 1.33:12,04; %Cí--4.81:4,45 ESk/EM);
Rmpmeal formula* Cjí. 1-1.?? O N>, 04 [M >Hj! c dcniaíed: 70J Í6?8 jM dif measured: 701.2643 dimef Ιϊ>! I-I33-py rro le»3 -« x a m tde 1 ,2-dfmeíhyl4 ^pyrroíe^e&amp;rfoos'&amp;niiáe· ^ ^ v" ^ ^¾¾¾^¾ ί | ^-ift=?d^ty Ij 3j^ ^^^ 2{llf|"yl|carbi>»yl|pfei-m'!}-'A“C4'-feydr'üxyphea>,l}''Í^~<lss'iH'sthyÍ*¿¥''{|>ys'íá«)to¡13'' es fioisddde dihy<lroehioríd« 1 he tille eompound is uhisined m accordanee wíth the procesa oí F.xanipie l using the aeid of Prepara?ion i and the eoropound oí Preparados* 7' in Siep Λ, and the eonipound of Prepara! ion Γ' in Slep í..\ The eonsponnd obúúned is con verted i oto sai? form and iyophdistd as describid in Use genera; procesa to obtain the. Utie compound. Eíemettéaí mk'rtumeiysis? {%, fbmmiietíkmmmmd) %(:-6 = .0 i :60. i 7; 7Ü-5-5
High-re^iuihm maso specfmmefty {ESlF/FÍA/HM, ESiFFlAi:
Hnphieaí íbrrmda: 63¡ R.u Ή M? O.; jM = Hf ealeulaledí 73432 = 6 [hda-f-IJ* measufcdr 7743220 «Irb«ble dihyd roe ¡b Ior Id# 1 he ude cnmpoimd is ohuuncd in aecordanee wuh ibt* procos üfhdampl# 1 udag dt.radd: of Purpandon I and do i.omponnd d Pro para don s>' >n den Λ, and tho eompound of Preparalion i" lo Stop id i la* casaam-od obtained d con verted hito -íult loro· and iyophihoed a-; dcacdhed m tho generad procesa U> ohhn.n de tule eompouod. idkmmfaf mk'mmiafysisi {%, íheimnk'íd:nmyutred} %<-6 1.0 i :6! dtU; -5.74;5.(>*>: 12.15TI {-k °-»t 4-13.15:51,51
High'-res'ohitkm mass ípectrmmtry (ERÍ/flASBR end MS/MS):
Empírica í ¡brrmiia: <Y: Η.μ O d? (¾ [M-Hj* calcúlate*!: 734.3216 measurcd: 734.3218 yl}'vl/7'¡>yrnde'3''carbnxamble
Ex amolé 11H, S^aSddhiorod'd'lld.SdddcIjlInoroaHdbvlddd-dlhvdret.-sfainbutbn-dlIlfl--yb-1/'7"pyrrale~3''Oarhosaadde <f í meíhy I-I77-pyr re NkV «arfeo x o m i ck '1 ha míe emiipound ií* ohíüíned in aecordanre with ihe proeeas fsí Imam pie i using ihe aeid oí Preparación i and she eompoumi oí Preparación V in Step A. and ü\e compoimd oí Prepmoiem 2K'! m Stop 0.
Elememai micmamiym; (%f siíe&amp;retjcafrmetiremfí %G-6ÍPl2'67i:94·: %Η-5.Μ:5.?7; %H-d;L72;1! .65
High-resoinimn mms specírameíry (ESIkHM, ¡CS$~:LÑ);
Ernpiricai formula: C,.í; ÍL. (1 Nf, C K |'M s-IIÍ: eakedaied; 705.2651 IM-s-HJ' meassired; 705.2652 2Cl/7pylicarbí.Píyí||iirrPsyi}a5ó|4...||yílroxy¡OHajyl}o|G-c!iniídlvykGOÍIkiriñnnrpmalhyÍ)' 1 #/»pyraxoM»y !)·> til-py rsxdtk-xmrlssiremya
Kx»m»te US^^-Chlorft^-nf^SV^Kmftrpfaoltai^-.ytmrthyi^i^^djhydrcfeoqalgqMa·-2(U/)-ylJeikíi«>»yí ¡ plus»* !>-Ai-í4-bytíre*>ííh«iyl>*Af'*l I -(2-wifthoxyttfety!)-! #/-py rajtoK 4-yl j-1 ¿-tlimetiiy l- ti^pymdc^ai'bcíxuaaidi? MÍg^j^S4pehIertH2>.||<35í^morph(»l$n~4-ylto«tb3?Í}-3,4*díliy<lro$$oqiií«t»ilii. 2(l/0-yU«*HMmyí|phe»yl)-AK4*Mw»-V¡»h««yl>-M'<*i»e«%í'“/v”tH«x«*«»"3-yn’'llí· py*$iíéi*4-yl j« 1 hi. fíík coinpound is nhíuu^d ín accordance Hsc poseen ot 6\ a* ni: i ο'-,ηρ the m se oí PrepíUnikm I and the eonspound ot j're par a Uon 3! n* Nlep λ and tlv e^nipound of PreprMion 26,: in Step C\
High-rewluíion mma spertromeir? (ESÍ+/HM, ESi~/lE}>
Lnipírkul loop ufe:: ü4íi H4| C1 N,; 0$ iMHÍf culeukned. '>21 3000 fMtil]' urea sored: ?213>02 irample O?, S~(S”Chlení”2~||{3^-3-C^orphoH»-4-ylmeíhyÍ)-.:M'*áifey*lrolS0q»i»^|i^;· I(i/I}~>1|iarrfeonyl|plornyl}"^d’3'divdr<>xypliesíyi>-1 JH!i?«etby|-;¥'(!'-(let«hydn>foraP'· 3~y1)~l ll-|rvm?:ol-4~yi|~!í^|>yrro!«-'3'-e8rlH>yai!PÍrk' hyiiroehloride 1 he tuk compuune *s nln us *.3 r: record anee vúlh tlse ploceo of hxamnle 1 u* 'no iLe udd of Preparación I aml the lompound of Prcpannkm 5' ir» Su»p A, and the iPiipo«nd:M; Prepatation 22!< in Siep C\ The compound obla; ned is converted ínío aalt ferm and lyophiíised as desenhed in ihe peñera! procesa to oblara du? litio eonipound.
Eitwu ttittf mhi<tttmsh"ih: s ... fbeon-iicatmemur^i n C o* Si.^oV di ' 10 89:10.^.^0 4.5*4 n '
Higk*re$0Íutimt--mms¡-$pecfmmeíiy (ESÜ+/H8:, ESESlMf;
Empiríeal formula: í.\u C1 N6 C\ ¡M'l I!]: caíeulated: 735.3056 mensurad: 735.3061 1(1 #/}~yi|€srb»ay l| pfceay Ι}νΛ«| I ^2~feydrexy*&amp;meihy !pro|)y IH #l*py r«MÍ*4~yl!^ÍV^4« hyd r«s sy p h o*tyl)«1,2 ~d ira sí l%y 1~ I ii»py rr» I «ea ríw» *» ®tí«
Exanipie £2*hS"($-€hÍftf5>2"llEESV3“(mKypheim^"ylmtdhvlK1«4"dilmir&amp;to8Simali8~ 2{H/)»>i|«srbosyí|phfcíiyi)«í pyndí&amp;»$-yijHV~phenyMftr»pyrm!a^-«arbo$amiíie
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The. di le eompouml is ohiame» in aemrdanm sviih ihe procese oí Ex aro píe 1 usmg ¡he aeid oí kreparadun 1 mu! the conipound of Preparador 8’ io Stop. A, and ihe eompound of
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EssmbpE M5< S~{6-ll{^y>-3-lrl>iflm?r9»K>itiyÍES<4»iÍIfevdrftíso^íii«&amp;SI»~2mi)-yi|earÍi«»yl}''Ií3''b««KodI«sí>S~S-yl>-/V-(4''íiydrexypheüyl)'J»2»dimethyl'>'iV“{I“^«tfeyÍ“· 1 í/ - py r t'í ^ Ιΐ> 12 ? í - .61 py r I fl i i ^ - 5 -y i | -1 i/" |> v ~ ^¾¾ r ls« x a m í <ϊ íí
Exsju|}|e 3i^5~(é'4|f35)-34l>i0í3oríime$hy!}'3vl~<l¡hydriíis<>qyhieiin-2ilS}« yí|c»rh«ñyli-l3j4í««sí«di»x*s!«5*yí)''A'''í4''hyiír«syphí;»yl)-I,2“áImeihyl~A?'''íl”i«eíhyÍ''· 23-dihydr<v4lf-pyTTO!«í|23~¿í]pyí'sdifí'S'yl}''llE|n:míl«'-3“earbo.xsmky C$S$*3~( -2( 1£$}- yll€arb»nyl}*J3»btP?o4i»x^E5*yI)«A?*{4-hydrpxy.pfe«»yl^l>2‘dim«<fey!*AM1»f«Mfth%I»· l/í~pyr8?.«M~y¡)-llí-pyrr«k<Ee8H>$xaiiíÍ€Íe
Esa|)MílE3^546-if<;lS>3'<E''luf?rei5teflyyl}<43^1Wclrehe$pÍBsllii"2(li2|-< ^'“!*J'*>ro1o(23"é}pyri«ij»-S»yl}''Ií/-pyrr«b~3"e«rbo's.amkÍ€
Esainpl*? .M3, I (Cp y1|K$ri$oftylH34KWodmy«5~ylp;V^4"b>yyp£yphcspyíH3~<ti^yi*yW^<1 #μΙ1|1~ -{|(3xV}“3”|0»S)’'íIexali|‘ drbpf Mad««(2,1 k|fM]axsmss'd( i íí)“ v1rsii'll?yi^v3>4HÍil\v4rois«qiPín>lkV'2fÍi:f^'yl]«)<:ulK>ií\1kI J4H%Mí^dkisoi~3''yl)'';V--{4'' fmJrnyypfuípylP 1,1" d mudhv i » .-V- (i. - m zi ky (~ í 3(~pyra«d-d~yi}'d íf-pyry«k«3~ «arPssxsrpkkí
IiÉMfik 37 L 5"{é - {| (λ$>3-|f 9 &amp;hy ú ro py raQÉ&amp;&amp;ji2 4 *#| 11,4| »Μϋ»*β(Ι^ yÍimkhyl|^3H!ifeydrí4xíK^MrioÍip3kll/|"yl|c8r!mpyl}-!3*fe<-?»^^«<>kS~yl)-AH4-byiJrexypfmsy l)»I ^'•álmeíhy Ι*Λ-(! -snetliyl- I K-pyyr&amp;kj 23^ á)i>yridip-S«yl)--llk pyrrf>l«s-3-csirbftxa*»UÍe
IkaiBpk; 37:J<S'>lfe>ljl333-3'hÍ<i^3'dli‘x;kndrijpvra%lftfj|2J~¿.H1^ki8g:jji<3ílllV· y^f«éíltyl|'»^4-íÍihydreIsPí|«maüii-20iO~yl!«arb»ayl|-13“^e»attílkx»i»S-yl)'>iV«{4'· hydroxyphfcpyl)-! 3 ^IPHí?lyvkAí"Ckm«llyy!''23''^^>,^r<>^ ^“P.V rro^P3^Ipyndm"5-y l}~!. #(~ py md ¿?-,V c» r bo«» m idy E%mmk.m,^íéAim^rnngU dxkil*yi¡k>pyrMJB0|23 >c| f I ,4|οχ&amp;*Ι»-8(Ι I?)-ytoéíÍíyl}«3,4'^lihydrí}isoq«í«»ÍlP“2(l£ír)-yl]€srbo»yl}»l3'*beií««cüo5«l»S«yik/V''{4"‘: bydrsyy pbsmy tp 1,1-'d¡mekyP;V"( 1 -SMdhyP! H -py mm M -y i) -1 l/-py rrole->· yprbpxpípMs l3;MkPki24S-'(<k (1(35)-3- [{9sJ?pIf gt&amp;hydríípy rmdt«|2}.1.-c| ¡ 1 pÉ|»tM*«dií 1llp yimeííiyi|~3»4“<iihydniisoqaítift!íP'2(!I#)“yl|«:ai'feo»ylkM”b^»*o<lioxoil»S»yl)-<'V»í4v' iydroxyptmsyip 13”ddíHdhyl“5-(l -meffeyl· 1((- py r rulo \ '23-k] py rtd k-S-y I)-1 31-py y rtt k"-3-e&amp; y boxa mkf y
EmamSt 3?.$> J »<?} 11 >4fcMlSst-&amp; 1HV yÍme*hyl)3M“á&amp;>'*íirolsoq«i8oli?i*20£A'yiie&amp;^bo»y1H3~k«8*8dfoMb5~yÍVM{4*· hydmxy|>líeíiyl>4»2*d¿mfeíhyl«AM y I}-1 íl" py míie“3~« a rfeos «mid e Í||Sgi|dt3JS^"C^'4IPx^>vid'Á8TÚ$iíimeíhyl)~3>4'<lih>xlreh<H|i3h'$<4Íi$i--2i.iÍI)« yl¡e»si>o?sy I}"13*fe*»*<ídf0X8Í4S~yl)“AH''4"feydr«xy|dH:»yl)''l 3~dsm«fhy¡-iVdl -sspffbyl*· !/í'Pyr»2ol'>4-yl^1//*pyrroÍe*3’'Csrbo«8i»iíle 1#/*|»ym»bi|2>3"b||>yrídíii'\Vyií-l/f«|>yrn>le“3-«»rii«x8.míá0·
Emính 373, 5 36" | ¡ OS) OSA mi n « sind lw i KM'-difs’V d ro ksKf sdsso ti o~2f 1.71)-yÍj£»rbttfíyl}~i3“k£»*t>^bmd"5'yÍ)~3^4~hydroxypfee«yl)“J*2HÍImstl*yl“3MJM&amp;441iyl'’ 23-d iby dr^'d/Epy rreldP py riáli^S^lb 1 fed3^«s rfeíma ipldé
IklJMIlfeJJls C&amp;-! f{33>3' ff3»€yíme$y?.«l id m»! * y I) mol fyylj -3,4-dsby¢.1 re msptedtb-2{l/7)-yi|«ar Ικ>ϊη i}~ 1,3"fc^*«d^^«b5"yl}~Af“(44í5 f4rexyphe*sy I)-1 3-di*»«dfíy t~N-( I ~ meífeyMl^pyrMoM-ylHIsf-pyrroh-SK'íirbesamiik
IkMMilkiJE S46-I {(35)-3-1(3 ~ €y ss sso&amp;r.sd id i»~ I -y i} m sdhy I \ -3 ,4~dl §sy sí m kop a i $3 ssIM-2{! 7:/V-yljess ñumy 1} - i 3 4*is»£odis>rs'd3-y i )«N*(4-hyd rory ptieay I)-1 3-dbssrtísy Ϊ-Ν41 · m <d h y I-1 /A py r ro lo |234>| py si ú mO -yl) «1 #3py rrs> te~3-es rP swem ble I-ktlllilit. 3fTL5 '{6·« f 11' 33')~3-f {3» Cy § amrrsdkl its- I -y I psie f by I\-3,4-di i?yd ro ssesp lis «>Ísíí-3C1 /.l>yl)ess rbossylH3*be»**idÍos*d-§-y i W¥-<4»hydrexypls«fty S)-13-dia®e*hy t-/V~< i -sBidPyl33'dd3ys.lrs>'IÍ/'-pyrs'í'sbs|23"/s^pyrklm'S''yll"l#/-'pymík"3"yas'boxíss«kle
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Eliililltt 513, iV~í 4" II vil xypl«:; uy 1 p1 J->d»dky!--77p1 -1¾ $ iliy 1'1 ll-pv rmhí ¡ 2 3' 3 1~ py^idh^S'>'l)'3v|{>'||{331''3'3l'«^í^Cv'a«sss|>iro!3,3pM'p^'é''Vlmetirvl)N3;4'i!ifeyclr?ífóí;>·· pu ku.4s u »3|! 77}>;y !1 car b o siv !| 4,3* b««íá i pmí t-5-y 1 h 1 1·/~|>ν rrn! a-;Wa rt}«a sa I é 8 £MBtíBÍSix^C$»Fto»re-2»f|<3x$>3^mWpW4^IS^hyIH>4-dÍ6^driátewlsells» 2{ 1 IIKvllesrfeí>ís>3) pÍuu>yÍ>~Add-hydrurv pisen y l)»1^-dsmeíiryWV^py mimd-ytpl H~ p y r m k-3 - ess yb o y am M e hydroi'hloridí;
Ehimenfat {%, theimgkaEmemsn'ed} %€-67,2S:S?,73; %f-f-3.65:5,% %:N-f%GÍ>~&amp;09:5.79
Ebussaple 3B5,liC3S)-32^orpholH^4-yÍ0^ftyÍp3,4-31hv3rok^ipdB0lte'-2CIIÍ)~yl|€^íi>oíyyi|f>!i£í?á>3)~^i-^4“hydr8.i^fphfpyl^!s3^dim8Íh|;l^{l»8ietiiyi»íll~ pymdof23'^yr*dift-5-yIplJ:l«pyrr8te«3<&amp;rts*mss*'skk hy<lreeiI«rMt>
High~re.\úfuíwn mms speeiromeirv {ESh}: limpirica! fkmsuUu <A- il*5 0 57:-.¾ |Μ!ΉΓ calcúlate*! 7! 3.3253 |M+H|' measured: 71.13372 Í2MIMilll.3Í^k 5-CS"C13<>ry~2-l|(3S)-34.ronrplii>Hí^4~>ime$liyl)~3v4~dlhyd:relsnqyi«<3hv SClíO'yilcsírhímy^piH'SíylkA^IS'-síysjfio-kl'dmsel'kyklll-pyrrftlk^ylkA?-^' isydrpyypfeepylH^disseíisyM^ byárssdsjorMe
Siey A: N~¡4 -/ή?#?- B uiyHtftmeih}i)$ityjfoxyph emdf- 5-/5- chidm ~ 2 -^38)-3- (morpkoün a -
meíkyl)~SA<idiyiUH>-4ll4smpEimúme-2M:ürhmi^iEHmyí¡^N~i5~€yimih~L2''iümiHh\'E nyrfú¡-E-y¡\-4,2-'MmeihyEpwriik~d<mtmxamiúe
The iUk· eompound is ohiunic-d in accordaoce wúh i he proteo ofLxample l usina the acSd M' í and ppmpoupá # Pjregaf^jp* 1' ip Biep á.? auá ite eoippouud ·*>£ skcpiiraüoíi SH<! in dep C, IRi v -CN-: 22!0οηΓ; v -OO-: 1631 ems yijfi'jtrbimyitpiteftyfl·'^i$f~p\ ? 't-huÍn>xypL·nyí)-L2» émiiíkyl· i H~pyrr&amp;k -i «eiuhmmnMe kydrochi&amp;rhie
Tho compound <>f SU?p Λ ís dopíoloctod ift aacordance vvíth thc protoca! detóocd lo Step t> csf Exampic 1- fhe producí thm-by ohtalned ss flnaüy subjected o) a stop; 6lf aübv v> v ?! v s a* !o*r? <o *í o w> u! 1K 'i α v \ιΛι o iauon and v ohdn>alv m a mixture oúa.eloo9nleóvatc;\ iho expeciod prnduet l·: obkueed. li NMM ÓOO MH,\ 5 ppor 11,2 tks. HU 9 « tKsJíh, ; 63 ,d, i Hk 7 54 uk 1 H);: 7.33 (% I m* 114 0«, 2 H), 7 (m. 2 II k 6.8 Cd; 2 Η), 6.62 (d, 2 H). 6,57 (ha, ! «| 5.26 ko i H), 5.26 |ms I Hk 4.64/4.03 (AB( 2 H), 4.01/3.92 (2m, 4 Hk 3:75/3.43/3.15/3.02 {4m, 4 Hk 3.5'* Ok 3 tt\ 3.3/3.15 {2m, 2 Hk 2.07 ¡s. 4\\\ l.t#QS2 (ddKi, 2 Hk 2.0* !>. 3 1% 1.91 <s, 3 H)
MtemimMi micrmntífysti: (%t fheoreiieaímtemuréd} %<MH.34:65.30; 14(1-5.6.::5.1 5; ;0>N 91,15:10.94 %Cl-»4,?0:4,44
High-resüíuikm mmt iprcmnnem' (i SI+)t EmpMeai tbmmlb: Ü.p/B|s 0.1%(¾ p t lilf oalepiaíod: 717.2952 {MfHV meosured: 7) 7.294 i iSxatBioift 387. AK4«Mydrox>· ph«ny!H *2**d*wi«í by Ι-ΛΜ I -meíhyl- l/#~pyrroIoj23" yIlc8?'b4?>>4^1v3n?op^p|lox<'sb5~yO“17/~pyrrakK3-oarl.>«amlcle kydro«M»|$jN¡ Eíemetiífif mkí'onmih He' í V th* fi^dctitmemured) %í>66.62:66.36: %H-5 59:5.62: %Nb=10.44:(0,72 %Cl»-4.57:4.55 ili ρβϋ#Ι’ {KSí l·}: fmpitksd iommiu 1 jM-t-H}* caknlaíed: 73A3239 |M 1!)' mensurad ''3d3?4i |{'¾^¾¾¾ííi¾^3¾i,S4i^;i¾l¾rc?<^2"lll3χy^^S'(sn¾mh^Iia'4-41meilvvíí''34"¾lli¾¥llmls#¾:níi¾θik¾' íii^yd.r«~IM«pyrrQKi|2-3~^!i^yris:l«^S-yÍ)"ill“i>ym>kx'-3-€«rlHis:«wye dtbydrocliloridfc The tille eompennd is ohiamed in heeomaoce with ihe proeess describid in Step B of Exampie 49 using Example 71 as xtarting material, it being undersiood that the produetis fmalis subjeeted lo a step oí conversión into salí íorm in the pr escoce ο! IΜ HO in ether. Afier íiHratiou and lyophilisation in a mixture of áebioniiriieMatetY Éé£ expeetiíí oompoond as ohtained. i'fam&amp;tNi mónmmiym: r'»« thwmimhmmwm'é} ,i3- o2 Í o2 o#,,00*1 > o i 4 ON \ ! 0 4* i o E\ f λ 1 Ϊ' 2 U 2 9|
High-res&amp;itmm mms spectrumeíry (EShos Empineai foronda: i.o ¿ Us *21 Νλ CE |M+H| calenc-.tc-d'. 731.31o? [M+Hf meaxured: 7)1.3m A:itlurivd.«||<3ál«34m<niih<>lh-4”yloH9lwl}"3.4"<lihy<irois<i<|idnoliu-'· 2il/f)~¥l|carbaoylBrhcoyl}''A7(4d.rv'droxypb«nyi}'-ld''di^tdbyl''íV--'tI'Unetbyt''!l:A |.>yrrola|23~<1lfyt4díivS*yl)-!M'|iyrroÍc-3»€arboxaattdc bydrncblcrkle 'The procederé is in aceordanee with the general protoeol of Exampie 1 usina tire «pprpprtaie iheparaiiuns. Thespmtel obtuinedm intuí h disolved in amomtrile and converted into sal? forrn uslng OJM aqneous HCi sohniom Adera iyophílisarkm siep. the cxptcíed vompound Β obíained ín the isam ofu -adnl itiemeníai mkroimtdyshi {%< %C =«65.88-66,28; S3:>. 15; 10.08*. i 0.95; *5 át'l-M .63:4,47 Bígk-resúfation mms speetmmeirp fE&amp;S/ElA/SIR atul
Bmprlea! fbnnislav O42 IBrCONs (¾ iMítif calcúlate!: 729.2951 [IvN'l!!' measurcd: 729.2954 3^HMtihL^í^MCS~<''hl«r»-2-'||t%%x)'*xNm<>rpt«)li»*4«ylmelhyl^3>4*dihyár»iseqalm%iS* 2{17#}-yllcarl:»'ia>9|plH^)>1hB.2-99iifthyl“B5(Í;*mi9hyBI72"pyrrok92B's9||>¥ri99s~5“¥l|'' i'V~ p 6 e ísv I-157~ pv r r« k "2" ca rbe s. asas iú a
The proeedure is m aceordaoee wüí¡ the general, pnttncol &amp;f Ox&amp;mpie I esing ihe approprktfe Preparativas. it bdvg underskxxl IMt -§&amp;p. 1) 0 n«si éamed out. Tbfe expeeted prudííat is ohenned sn lae !>asc la-roe iÜemmtehnk'rwmüini.i; {%< ihenreiiaii:memntred}· %C>7C!.?2;69.?7. %!TO.?9:>.96; %N-H .7011.43
High-resoÍníim mms spetfmmetry {ESI*}?
EngnkeaJ. Iwmnis; C.¡¿ Iip: 01 0¾ (¾ [Kdedí]^ calenlaleá: 713,300! fMOHf nOásered: 713.2998 IBsmnte 391., 5kSkllikre~2- 1 ¡ {3O03Hi«nrp!rell!v4'n4«H4liy ll'-deOdlhvílriHsnfielnniln'· 211 £?H‘l|cart>68y !l|>lienylVB4(4^y<1roxyphanyl}~I v2nlkn*kliíykAM I-sm;tf*yk 1/7-py n'nln|3>2~E||>y rldln-S-yi)" 177-'|>ysrnkí'0««nrónMaBde |iy#rpefeI»rliÍ0 (?«» ánmretíí'üí;mmsMtmí) %0-í>5,88.65.69; 35! 1-5.33:4.8?; 9.0-10.98;!0.86; 0011--4,6300! tiigh-reseitífion mms specmm(!if¥ fEkt^OÍTÁjO Empklaa! fcnnnl&amp;: C2« H<n. OI Ms 04 cdemated: 729.2051 ÍOt-í Hj : measumd; 729,2953 i|i«:nofl0'\?4Í.II}')1|^8rÍ.HHív!||ilHrByl}'A^44i!yílr0x.y|>k€^yÍ|"!,l"
Ekmemíd' mk'rtxMíttlvsis; (%> Úwútef¡aii:meimin;d} %CMI; 19:63.33; %11-6.00:5.40: %HSK!(X?li:!0,7!; %G---4.55:4.46: %C! ->MM.H. xpedmme^f pSJ0'4MÁ;6 Empinca! lomuda; C.j » Hs; C! N> O; |M>-ll]‘c:Uí:ulalec!: ?03 3046 | Kl 5 ,H] measured: 703 .3((42
Eyaiai4a343,4'4S'4';01ar^'-2'4'H3á3-3453'4>i'a.ír|4K4(»~4'a44>TOpvIl--3>4-4i0¥cIraIsa··· <p ki olki-41 !£?)> y I jcarbotiy !) p fe a«y I }4V44'-hy dra&amp;y pfe «syljei k/V-( í»m«$|iy!* íÍ/“l>yrroío|23“^Ipyritim-S~yl)~I/f“|iiym>le"3“C»rííoxai»i«l« Imlreelderlde {KSl-WPIAk
Bmpirkal lormuía: i ϊ*?. CI5½ (¾ jMM!]' cuieuhued: 752.3354 ÍM-H!f me asa red: 757 3265
Ma¿Eal$.i2íts'4S'C:hi<>.$s>x2.f|(3.á0'x145^‘43B«r|aha!¡n--4--yl)propyÍ|-3<t4« dlliyárabaquInolln^l/^ylIcarbüRyilpfefa^l^i^l^ycIrüxyiíheiiyl^l'^ai^^y^ (I~a)ethy!~2v3'íllhy4ra~ll7~py.rrala|3.33'2?||nai4la~5~yl5!234|>yrrí>le~3'-carla.s.5i5aa>ií(a 13m (Ole eompoand la pOiallKíd la eceardaBca wlth the process describid in Step B of Isa&amp;aípte 49 psldg Oaample 333 as staftipa amOoiai, it bei«g umlet»iu<xf (ha? (he produci (a aoi siibpeled (a (Me slsp dlmPOMrslPbdMo aaM llaim
EiemeHMi mk-rmin^fy^h: {%, ihi'urtfik'aírttteasíired} %C-6v.60:é9 56; %H«C2t:é.34; %N::4 ! .07;! 1.08
Mígh~n\w$uíúm mms íESIt-dílAT
Empirkiíl CTnIio CI N#<Ü<» [ M*H j * -éatóibfeá :759.3420 im-Ηγ mmmm&amp; '75*3422
MlllllM3ÍSv5^4oA::hk>ro-a-Íl|3SKl4:BSioi>lo5lis^->lMi4lxyi)-3,4-'diliyílr^ís$$qyh.toI|B~ 2{!#l)-y^carl.w«yI}pÍM«yihA^3H:y*5H>-4~í»®^<s.wphewlK¥-{4-Fy0r«xyplM^yi)-l;|v. disj®tÍíyl'4ll“pyrro!^3^rfc«ssí»M^ liy^rps!ífeyii«
The procedure i': m accordiuut: wüh Ite ienupi proíoopl oí Imsmpk Tí ufpg tte appsopnak1· Pni'JKííuvtó 'k- prodwí ohumed is f«uH> díspaHod m meiomnrn and uomoned mío :--.51. u>*m u-nn^ OJM -.v-jucoo-; IR I solideo. Afee a kophüiáatíon aiq\ me e\pee*cd eompmmd sx oR-.med m the Hon ol -¾ vdkl. ílígit-teseimímt mms spi^mmmy ÍESI/PL4/Iii£ aad MS/MS.K’ * npmeal io* eka *. ¡ ίί$Ή V$k [.Vít-ΗΓ caladaíed: 730.2791 |ΜΗ!Γ oiessured; 730.27*4* §lilll|j|e3|4 iV»|3»Flaoro*4-hydrftxypSi«».f 1)-1 .rasmia yÍF5T^I|C3-FF3~{oo*rpl«*iln"4~yi.*«iT!wlT3í4-dihydr*>ismpiiKddvacI7lf)''ylK‘8rt*onyi|'' 1 ll»pvm>k'-3"«4í%lydsdldf l*i|#rp«feÍi>rMe i he tule <.omp-oynd e< obmiíkd m acvo'd.mee ooth *he procv^ o* Example í «s*n^ Ote acsd oí Prepurotíon H and *TV)- '-sv4mtorphoimy!methylV 1.2,3 4Aetrahydroisnquholíne (see Píopataun'» ,Ρ'ϊ h- Stop V and «he eempomid oí Ropa* aneo 20" m Stop ( , Uto psoducl ohimned ís íirafA subjocted to a xmp o! conversos*» isdo salí funn ut íhe preserve oí 1 Al I !d m ether. Aíler difundo and Uophidsadon rn a mixture oí neefonifrde/eo.um\ ihe expeeted componed *s ohiamed kkmmttttf mk'nmmtfym'? {%> ih e^reitaí^memureé} %Μ5.77:ί>5.43; %11-5.39:5.1*; %N-t1,31:1 i .21; %CM=4.??:4.34
Migh-re^üimwn mtm spearomeEy (lilSI/PIA/llE «fl lEnSpmcaí formula: C>«> fe F O* i[M-HIJ' caleuíated: 707.2988 :[MoHf meawred: 7ñ?.2m |Cggmp|c 397, S"l54d!ilora-2-fl(3>yh3-'im0rpdjilíii"4^vfmeifevÍW3x4'dik¥clrolrucj;ofooit^·' 2(1í/)»yl|c8rh«*iyíJphe«yl)''íV44''feydrnx5fph«»yljh^'l2“métliiasyp3fS>ii«idÍn^yÍ)''!,2~ <fimcthyÍ~lií“pyrmÍe“.Vearfe»xsmkÍe
Htgh~re* ítimiftn ma r.v spectmmeiry mmn &amp;njkíida! fornmlá; Cj<j 70 Cl! &amp; Ck fkf ; I If caícoíatcd: ?0?.2?43 ÍMrf ir tmmitiMi 70?.2746
Emroídr 396, 3'(5-4!llfdxoa>'-2klklfy^-k.nejid;n>ila'-4-vkrn^fevlk3Jolll^vdrcda<:mid:n»!hi.'-illiíkyllsarferníydlpluwlkA^^^ywidiimiyd^lV'dd-hyilroKypfeepyd^d^i^lPietiykl#» pyrroI«-3~ mrhmamidr frydroi'klsri4r
Elementa! mkro:vmdyO;: (%, them-eifcui:mm%ured) 00-60.85:66,75; %! 05,34:5.42: ON-9.51:9,73- 230-9.62:9.67; 000-4.0:4,71
High-reuiiuHím mm* specírometry ¿.ESΊ : -/7-7.4,77.62 ESE/FIA};
Empírica! lomuda: Cu i O O NU O,;. I540 11" eakulaied: 700.2685 [M»Hf measpie# 700,2616 iytmmpte 309. S-{S714duro-27l{3,S4-¿kim(rrplod:kr-4-o-1^e7hvl>/k4-€lili¥drokutiuÍuolijr-2{177)-yM mrf.KmyÍ| plseiívikA'-k^&amp;iorU'd-hydr^ypliesiy ik ldMlmeíltyÍ~7V~{ I -mtdtsyk 177- pe rm.n M-y 1} - ! /7 -pyrro kOOearl.H: s 55 m i d a tmtroeh I o mi &amp;
The hile eoropunnd k ohuaned in auusuianeo wuh ¡he procesa o?' Oxan-pk 1 uOng ihc acid of Preparaikn i and 0\5>344"«H>rpholiuyíme4hyiV \¡23»4-íctruhs droiso<$uinol me (see-Prepararon 3') lu Step A, and the cnmpound oí fkepatatkm 20“ in Step 0. The produci ttbinined *s Hnally suhjectad ve a Msp o 1'conversión into saU íorm io the presence oí'IM 1K1 m ctíKs Auoí íüuation and hophdivnmo m a snknae oí acelera?: üemanrr, Uu oxjtít kM *. empmmd
BiMmmíei f%:-^mré^0imemareá) %í>62.21:61.32; %H-5J6:S.i8; %N-11.46:! 1.14; %í> 9.66:10.i6; 3202-4.83:5.23 Jíigk-rvsoiaíi&amp;n mass spe€trámeiry-(£SH-/FÍA/ÍÍRt ESF/FL4 )? Iñnplnoai fonvnua: (.' o iOe O 6 62. O.; i MvH |" aileulatcd: 6902700 ¡P3- H f measureck 697.2704
Examnte 400. ¡Vf eihy i 2»| US-(5-«Wer#-2* 6y<lros.yp6coy26«BÍ«n|pyrldioc-4H^rÍ>osyÍ»sie feydrodsiorkie
Oie title compousp is a sposArv podpoí wbich fcrms: m tte course oí synife#sis oí Exumple U0 (m thc fimü Síep feefore (he stop oí conversión loto sal? form) owlpg i» hvJro'>Ms οΠΟο nUrUe ínnctio» *nk> a nuthvl.esicr nmoüoo* The comprniod k Nqv»tau.«i !Wm Ovo oíOxample 110 6> ehtomrnoyraphy owr sihea ed in a msxinre ni mdhanol and dichloromcthaoc. %C':::63.60:64.43; %HdS.3?>:5.0h %6k0.06:9,34: %€Γ--4.60;4.46
Migh-resüitíi^mm^m^0mv^p· ESF/IR): í:mpirie«l íommkv. C.<; H<;; Π N$ ik {MH!]* eakulaied: 734.2740 j' N i Ή-I j *· meas u red: 73 4.2 743 EEmemaí micrnimniysLs; {%> theoreiicüi:measMreii! %CÍ-"Í|.IS;64 J3; %1!g967:4,7!; %NG.24:9,36: %G:-: 9,4:0:9,59:. %G':::4,70:4.S0
High-mukm'km masx specimmefry *97,57 *·~.ΨΙ4/1¾ E$!~/FíA}íí Empliksl feMHifi; Cm %·? €4 f 19¾ CO [M*Hf calcdamd: 718.2591 fMflff measomá; 7!|,1|93
Elemei?M.í micro#nofysis: {*¿, úuoo^ikcE:memuno:¿i %C:r65,20:65,92; %105.87:5,78; %NG 1.13:10.36 %€G-4.69:4.?9)
High~rmoluium mmx spetirmnetry: '(Ε$ίή$ϊ Empírica! formula: i'.;0 0FN»>0* | ΜΌΊ |: cabulatud' 719.3359 measureO; 719,3302 miel 2(Í7fEv1¡carlu:rn;yl)plH\U:ylr'A:'3’4'4.:y0roxy|>lo:o)4)'^is2'UÍimrl034-'A9U|,,||3|? or Λ')' í«írshyUr«fyira»“3*yl|~Í/í»pyra«oi-4-yi)''l/í-|)yrí,oíe''3"«srb«xa«íitíe Nydrrfshioride
Ek/mem'íd micmsnafy'iis: {%, theoreíh'izL'tfteasijurtuI} %C3-0J::10:;66:,04| %11-5.87:5 87; 61)90 11,13:10.62: %CTG-.69;4.?6
Kmpineal lomuda: OaHjd'iSkOs {M+H |’ eaícalued: ? 1 <U >S*>
|Mt i I \: meamtred: 719, 3 3SO
The componíais oí Bxtanples 41)2 and 403 are obadned tn aecordance oélh the pmce:a; o) Fxampie 1 n^ng íhtr acal oíPstpsratuiít S and uv e^mpound oí P'vp.eaoou >’ m Stop and the eompound oí Preparaban 22“ in Shep O. The díasterenísomm uh lamed are sepurafed h% oblad ohtvnuuographv and Oten eonv.sted arto t-ah letm and Kopla.ssed as dcaudva 5;! the general ρκ\ν^ lo \ khd Ote tole a>mpvutnde, llnmnk 4Í4,i»í§^C2btero^>-l¡iMi4^^drpltÍÍ^I~Tl^lbyi>-3ei~dIltydiatÉdióÍbéÍio-ipií>yl|esrboti>:líphe«yI|#feíl-eyaaopyr|i0íiip^f|)*eít?*{4»hydrsaypheii>'l)-lUi;· dimetln h 1I3~§n mde»3 atarlas s&amp;snkk in drneldtmde
Examftb 405. S0t1-431bIara4-dlC3#4T304norpltoÍio-4^¥Íinetk¥lb3P"4^0T0^^^<Í^óialio-^ .2t2ll70yll«arbia5>4|plten>1)-Iv2alteetl*ykdd-(l~sietbyÍ~llkp¥ríxtlaPv3“é|pyrklia*S-y1i" iV^py rMswist-4-y l)> i /í-pyrr«í«-5-eai4»fixamldle dlbyilmekiorkk lite tale cotnpound k pftói^oíh: accardance wiityhe process oí Fxarnpk i iusmg Ote aeld ot PtepataOoa 1 and íhe oompound id Ptopau uou V ai Step V atul 0 e appn «pítate trena, in ívtep C;f: Olio Step D k aot carried oud The eompoand pbtttoed ® convertid mu» salí íorm and íyophdKed «§ described m Ote general {Sroeiasi to dhíatít Ote Hilé conrpound, iltgh-reHihitkm mtm .*pevíromefty {l\Sít~AFl í/7iHf ESiVFÍA}? inipirkal íorraula; €4η 1l.n¡ €1N* th |M*I1| .aknhuej Ί< [Μ H ff taasSOred: 71522999:
Exímale ,í-dimeihyl' 1 H<*py mi 1-3 - y 1)-A'- ( 4 4iy 0 r a $ y p h e rsy I j-! ,Ί»
íliiíielhj'l'S-C3-'||(3SK'%-(sierpliO:ÍI»'4*yl»ií5ibyÍ)-3?4-clÍliyárolsrí<|tti«o¡l»''2<ÍI/jh yl|csrboftyl|pÍ5¡eiiyl)''!l:^p>,rr'o!^V^rfe<>x-4iMkb hytlrocN i sv Sirle i empee tul s- obmínea m me-dami ^ith ihe procos o: Γ'ampie 1 mee me aeid oí Piepa» >uo» and me eonuOimd of IhoparaUon *' m '¿♦«ρ and the *. om|xxm4 M. Proparaiion ib" in Step €, The oomj>oum.i obtained is eortverted huo salí íorm «I Ivophilised üs >ΚνοπΚ'Ί hi ihe peta-raí proeess ίοβρίη the tille compound f.iemeíiñti mierrnimiims! {%< íheotsík'aL'measared} %€-68,40:08.2/: %ÍN6,03:S.1.1: <><>N-11.68;i 1.75; %€Γ-4.93:4.?3 }Í?gb~reMí$Méti· mmF$^m€írédtét^0MÍ/FM/HM muí MM/MSf Bmperie&amp;í #ptuls; €&amp;; %? N<> 0<¡ [M ; Hf edeukfed; 683.3340 [M-i Hf measured: 683.3334 EMIllIllt.ill2v^9.3''FI«íjr€>-'44iydrei'>-phenyl|"S"<S”Bietliex>!"2»4i(3Sp¿^f$Mer|>hQli$^4>> 5Íiix.'ihyí)"3í4''dthydrotxequhH>ltti^tll/)-y||earl.H>n>4|phe»yiplJ~itimeth¥l«i!V~{l'' methyf* I //«pyra&amp;ííl^yipIJ?*|>ym*!e*3*earbexamide hydrodileride
Fhe tule componed s > m<ed .n .kxouhme vum i< >'K>u s »9 inmole vü!'L.dt u ¡p '9' Pumarotmn '3 and the «empaimcí m lOeparaneis v ai Sonp A, and the compeled oí' Prepamtion 20" ¡n Stop 0'. The mepuund ohsaineü is convente ti mío salí íerm ami íyophiltsed as demOiad in the genera! procese to ohtain the sitie c emperna!. {%, theoretíe&amp;tmemttred) %í>64.23:63.94; %H-5.80:5.00: 1 >52:11.56; Μ;Γ-4.86;4.99 Mígk^mmiídim {ES¡*~/fr?A/Hít and MS/MS, ES.h/Fl4); bniphícal íbnmtia. Cw H,ii-N(Ah (M+M| Citle.uisiet.il t>93,319.4 |M'H)* nicsoutxxl. oe t mi >‘M^rtmeyHplw»yihÍt2^Í*^*fey*-*/^yrrol^^5^rbo»mUI*h]y<lw>chleriae »bo otk' u'uym nd v oHo*rr I m**won}n8*?« v>tt» >v ρηχόν* ol i mt'.v n'c 'lit *> i *> Γ qt< *u> ι\ιί >c von poíhu dPopn.v <* 5 i Stop i .nt< K ,λμ®«η« ^paredón iir in Stop C. The eomnound ohtaíned h converted imo salí, fomi and k*H'hO\ed as dcsenlxv in ihe general pnvess u> <4>iam toe til te oompound 0k Mmretimiimemmei| %0«67.32;6730; %B-6.05:5,2$; %N-U .22:1 i.! 5.; %Ci‘“4.?3:4.59 , High-tesvtufion num specfmnetrv (FSir^FMMBMmá ESί~/ΠΛ}; íimpfrkai formula: (.'^ lio No Oj : fM-Hj' clfeMp]' 213.3446 IMHÍj' nteamsmP 713.3443 K^amglf?409,jV^4~HvdmaLVgfefayl|»l«2»áít»»íliyl»Ar»|I”8»elliyi"l##«pyrr«lnil33^1“ enrbonyi)ph«mylH H~pyrrate~3«ef rfm&amp;mide bydiOefebrido 'Oto tille eonipound is ohíained in aceonknoe wüh íhe prucess of'Exíunple t nsing íhe aud of Preparado»; 25 Md tle eoniiidódd of Prepararon 3* in Síep A, apd ilte ^nipnM ol Preparado» !!’ ín Stop C I - te lompnund nbudned o; e*nnerkd mío salí lene and tyophiHsed as descñbed in ihe general process ?o obtain the dtie compouod.
Eiemení&amp;l mk'nxmatysk; {%, ihmreticuimtemursé) %C::'68.98:68.95; %H-S.93:4.?6; %N- 11.49:11.43
Empirícal tomada: (d.> lieNMXi i M *Tlf' ealculakxd 695,3340 [y tMf measured: okxd'kit pyrrofe|23^)nyrí<íín-5*yl>-S«(2-{f(lSí''3*(*«o*,|»^<»lm'4»yIm«íliylM*'MÍbyáí^»«‘. <l^hs«lls^Nl(!IÍ}''yl^^íÍH>ísyi|phemiÍ}''lM''pyrrí)kKlH'ark>i«i.>ikk ii>nir<Hd>ioriíte The litle eroripound is ohtained in aeeordance vvith th&amp; procesa desetibed in Step B of E^tó^le 49 uái»g E&amp;ample 409 a§: aíar%g-'»i8ferial; The .eompound obimned ts con verted foto salí i>rm asid lyophiíised as desarshed Ib tfee· general graneas to ohtain the ode componed.
Migh-n’sóiMió# mms spmímmMip {fSSl/FL4/MMm.é£SiVt'lAk I impiden) formula: C.¡; B44 !d.<s Os f Ν·Β·Η)': ealeniated:. 697,949? [MTHf· tpeasured; 690497 ]^^|giÍlS»(5<hki^~a«||<3^>3-methyi--3t4-dltiy<lrcíh«^iilBeÍl»--2(ljF^ yi|earh»ttyl| phsoyljHT-C 4Bgydsmypheny ST^-meíhyWVT I »t8€ihyi»l IT~f>yr&amp;£«i~4-yÍH -|2din<rr|:d:ndÍe''9'yl}etliyTd7fi>yrraÍ<s\1''€arbeisredde bydroebtortde lie: td.k componed i« ohtamed la accordanee vvith íhe procesa of L'xarnplc 96 uslng the· aeid o i" Preparaüno 30, i he componed of Preparaban i h and the compoood of Preparaban V\ The vompound obubued is eonverteb imo saU form and lyophüised as describid te Ote general pnmvm íp-dhién the tille eomgm.md.
Higk-r<?$o¿ítfion mms speerromerry (ESi/FÍA/HR ¿mrf M5VM-5/; I Impiden! torunda: 1.3;§ HU¡ OI Nft O.5 !MMi| salcuiated: 603.2951 jMh-Hj' mensurad; 694,2947
Eiamwk· 4Í2.,6a'S-429d;ere'2' H(376T3d'3''{an>rrrh6Íhe'4BlieraesdhO,4BlIhvdreke··· q ahteIitiT.|ll:fi'yl|earI>ónyO|dvests1pe%9CS..e>eyned,l'alh»ellp'l'd#Tpyrreh\3''yÍO;Td4- hydimyphe«yl)”li2”dímeíhy?-li/"pyrr*le"3"«arbííaamids feydreeltlaride
The tille componed is obtained í« aecotdance wnli the proeess ηΓΠχηηιρΙο l using Ote aeld of Prepararon t and abe oomponnd af Preparaban. f7 |n Slep As and the compound «f INbpáíMicm l#1! ϊη Stop €·, TImí Géiñfíé&amp;éá'· bfeiained is convened into saU fon» and jyopMlised as desnrtbesl ííj tPn gongral pmcsas 10 obt&amp;in the Üile coropound. ÍCiemefüiií mteromwÍyih': {%< >0? no r etica !> nna? % u m/j %&amp;mMMM; M'WM6.2¾
Higk 'resofsmms mms s'petfmmefty (ESi/FM/HH and MS/M$t E$Í-/FM}r Empírica! fornida; í}*¿ Ha i-i Na ib yalc«)ated: 745.3264 jfo fol j' measurc&amp; 245.3.260 jkagaak 4tXAMS»€v»m.ol ,7- íilpaeihyl- WPS) - 3 O m a rp 6 d d-4 -y i m s: 16 y 1)~3.4~d Ornl m im q o fo a! d ~2 i 177)~y i \ -carfKmyl) AfoWsbydrm 1,4» Panand db a~foy |}.. | í l'.-py ran fofo ea rda s. a m kla Pydraatdsfod
The tille codfobnd lánOlatvieáiB aeaérd&amp;sieo wdlvtfe pmenM 6f Idample 1 üfojgilfoábd oí Prypíu'&amp;tíon ; 3 and fot componnd <>Γ Preparadon 3' d Sien A: and tho componmi oí Pfoptmífon !SM lo 8í@f> €:. Tíd foiápsond odafosd is ocfovbteá Into m!|. '.íppe. ífod iyophbafo na dnxordvd in ihc yañera? procesa io afondo foe üde conrpound.
EiémeMél.mí-ermmlpsb: (%t ikmteámlimemmeé) líigh--resoiuü{m mms speamm^try iESi/F!A/HR mui MS/MS, ESE/t'Lí} t Empírica) tomada· C.fo 0.« \\<Oc IK-HUVcdeuluSed: 741.3395 f.MMJ[: measurtd; 74 i Λ 307
EM81Ekili¿S'^"^'^^iS'y^'^<'W'34merphe1ÍB''4'ylmelli:yI)'3v4><lihydrfehe<|«byslÍB-2CÍII}-'¥l]€arH«s5yl||'5heíw!K^444syi!rftxypls«^}'1)4v&amp;''ilm«&amp;yi-Ar^lS-M^'fey|-SII‘· pyrT*de|3d^fp>>f^ldi«'l’">dMíí“i^¥?ríil«'h§*c&amp;rbexai«id«! hyáraehlwlde Ί he tsbe compound is obiaiuísi \n seocavb-oo wuh ;he proaem ot'Txamnie i aamg ibe add of Puipau-dion 1 nod 5he eompound oh PrepuuiUoo 3' ia Siep Λ, and he- appropñate amme in Stop C. Ί he oompnum! ohtaíncd h eonveried ínto xah furm and lyopMlised m desedbed i π i!® general procósa to óbrala the tibe eoiapouedL íitemeníeí mien>amiiy.\h; (%< theorefktiL'metiSfired} %&amp;^4Μαμ
Iiígh-'?e$oinfitm tmm speetmmeFy {ESi/FlA/i'M cmé MS/MS> ESF/FL4}:
Bopirkal íomuba; Cu hL;í O bb O.s [M-eMf· eaiodated: 730.290! [MTIf j ’’ mmmmÚ '< 1M. 29Ú4' leaampbafih, Sdd4hldara'2'Hilf)^dau>ridHdbt--l-yb»fethylb3^4->dihydrdCcHpdoalta·' 20 /O-yil^arlKuntfpl^^dpiVdS'-eyaiu'í-lílHliiyudhyl'' I #f'pymíl"3">ipA?"{4~ hydraxy|.d:iiadv1h'i'a«eÍhyC!l:Cp¥rredeO-earlH>aaívikle hydraehierUk The tale componed is obhbned m «acordante kicbthó pmeess pf Exáipple ! ibdbg tfie M\á of Piepavíitioa '3 asid tíre eornpoimd oh Preparador 3· m Sep A, and ihe ePñipóiihd df Pfepamtion IfT ib Stop C, The compoum! oblasned i$ converled tuto salt ihrm md IvophHised aa desetíbed Ib the general procesa to ohmio ihe tifie compound.
Efemetmt mtemmmíyniÍ: (%t thmrttfctííiiimmiiMé)' %Cb»é4 J 5 ¡OS J 4:. %íf ~f ,4 3 :|J4; %M::: TI .3 6:1 Oh; %€ T:::4 ;7b: 4 M7 Ííigh~n>wiufi<m ütm&amp; $pectmmeí*y (ESÍESÍ-/FM)! hmpirkal banana IV. 0.a.' 01 Oh. O.; [M+HJ* ealeuicPed: 7030774: s Mil í }* messared; 703.2775 Ι(1#Ι>-¥ΐ!ί:ΐ^ΛΗ>»γ!|ρ1ι^^Ι^^4»^ν^Γ»χνρ!^ν^ν1)-ΐ;ΐ~ιΙΐΜϊ^#ί^Ι«Λ'»|14ίΗ^«ΐ^Γ!(>'' m«tfeyl)“l#f“|»5fr»*«M''yÍ|*Iií»|jyrr«li?'“3~c«rb«x8s»iíle hydrocbtorkte
Fhc toso eomponre! i\ obiasned m üiore\lasn.o VvUh thc procer oí f xasnple I using tkoacki of Prepararon 1 and st¡e compouod of Prepararos* 3' m Stop .A. and the coHtpoutsd of Prepanstion 35“ m Stop C. Ote compoumi obtahsed ia convertid into sait ftmn and hophsksed as docrsbed ki the general proejas so oboko she tuk compound, :EkfM^MimkmksmiÍy\i%: ff¿« theerrth-aíZ-niemured} i#«Mí;61l4h%\l· 5,63*5 42; W 111,69:11.6i; %ΟΓ··;4.93:4.8|; Hügh"reo ?adoren mass spetitomeín {Ε8ί*·~;'£'$ 4/HR, Eki~/.FIA}:
HrTíphicíd b sonaos' Cjs. R;,!: C1 ¡>< N;. O,; l'M-í-HJ' taíeulatcd. 682.2982 Ifpdifireoassrmd: 682.2986 iMEjBMilL^5^Bthl^im¾lfeyl-m«pfrml·¼<l>«AK4»lιy<lmx><pheι¾fl^5.(4. nHklri>«ya2~ll(3A3a.k(ín<ni>linl«r^"yl^udl5yl)~3,4~<lilpvdrrnsrx|«innlín~2C!l#k f!|e^l^«yl|pirenyl)-í J-dim«íbykÍl^pyrr»i&amp;~3-e&amp;r6ft«amy^feydr««|lsr!|te ín aeowdanee. wiih the pnseeas of :^:8¾ of Preparatkm 31 and the coínpound of Prepararon .V so Stop A, and she oomposind oí Prepararon i 8“ in Stop C. i'he eompousxl obtained ss convertid into salí torso and; hopkriised as describes! m she gañera! proeess lo oblas;* dse filie erenposmd.
iltmh nh¡!mkmmiah^- ^a^tk'&amp;iímettMiteS %í>p2 3,2:67,56; %H-6.0S;5.84; 'ANA 1.22:1 1.2!; re<íT-4,?3>i.7i
High~resoítftÍtm mms speeírmmry (FFi/FM/HM mui MS/MS, ESiFEiA}; lúnpu'scni tornada; (..,<> 1!^ Nc„ O* f?v! HÍ]' caknlaíod: 713.5446 {M-s-HV ssseastaod: 7Γ3Α446 *\4~üibydroisoq»moli»»2CI H}~yl¡mvhmy'l\phmyiyi s2-áie5€tifl-A-|! «mcfhy M íE pyra«oI"4~yÍ)~I /l-pyrreie~3*caríi8xi»»«4le hygrocMorkie- I fu. «tic autspound 5¾ ^otosoed ni uec^rdanee vuth the procesa ui ίνηηρίο 1 nsmg use aod oí Preparo ion .Π and the eompowul oí iAeparatíon .V iu Stop A. ;md the comjMond oí Prepassstion i" so Step C. Tlie componed oblaisied is eouverted hito sah. fhmi and Uophih\ed as deseo htd m the genend emees- k> obt.un the tille eompuimd,
Mmtenial micnmmt^sm {%. Mmretküímrnmureé} 50065.86:65.51; %H»6.09;6.U*#; %NH 1.82:11.73; 860==4,98:5.14
Higk-res&amp;fm&amp;m mms spcctmmeiry (KSI/FiA/fiR, ESE/FIA}; icmpiricai formula: íhs 11« No O? : 675 .3289 | M : 11!; nteasurcd: 675 ...1286 ::fct$^íj^üSiAK3~(}>-aaepbe«ylk5-<$-fiuey6»2~<«3xS>34mor|>hoH$i-4~ylmefbyl)-3t4«Í' dihydroisoqumolin-Sílí/kyilearboayllpheRylJ-iV-d^hydrfisyplíeBylkl^-dimsflhyl-ÍIApyrrole~3~€8i1manside hytirochiorkle íílio ütle cumpound i« ohtained in aoeordanee utth the procesa ofExat tipie 1 aylpf íhe acid. ol f-hvparaüon 8 and the eompouu-1 t»í O p calmo y n> Stop A, m-d the compumui oi Preparador 4i" :n Step Γ. The eoutpm.nd obtamed s- vomencd cato salt fono and lybpMlMdas deserihed tn ílib general procesa U> obtain the ütle cornpound. 7/igh-í'e.mÍmkm mms spectmmeíry (ESF):
Empírica! formula: 0^1½^¾ jM+llj’ itiieuíated; 684.2988 [M+H]4 measured: 684.2975 2(UI)-yí|€arh«iiyÍ^hí5nyI)“AM‘i-hvár<»x>'pfeeiij-l)‘l1(2*<lii»i?tbyÍ»^-|i Tirkkn*fero>· »ielhyl|“!H-pj rajgoM-y i|»l £?»p) rrole-A-earboxamltie hydmebliirkle Γη o tule cono ound :s obtoíned h- aceosJanee wvf * Oto procos·* οίΓνηηρΓ ' nonp the oc*J oí PrepantUon 8 and the componed oí Preparatioa T ín Stop A, and the eonipunnd oí P sopa mirón J5'! m (' llv caponad ohfao'ed »5* eooxerted han -olí fono nal ! ophd· e> i Ase» bed nibopinw ai p > ^ w te η ί< nt tbo i d» conven td l$h;h~n\uihitwn /m¡o spt'kítfwwfty (t'SÍ''>}: i π"> ϊκ,.Ι vo ad t ,* ΚΙΑΓΧλΤ [M4 Π Γ enk'uhsted; <>nb J3&amp;5 [Μ4Τ!Γ rnoasuved: 660.3365
Riaaftftle 4B> íV-(5»C>en»-I Jlslimetbyl·! ^-pjTi^i'^yM-MS-fluero-a-Uí^SH'· 'Cpi»rph»Uii*-4“ylm«íh>'I)"3,4-dibydlr®isogwínoli»"2(l/l)-yiÍ«arlí«n>Íi}|jiieii>'i)”/v-|4^·
.. fcytirasypfecaylM
The «tle compound ts ofctained in aceoaianee with the procer oí Bxampie I utangíbeacid oí Preparatkm 8 and the compound oí Prepararon Ύ m Stop A, and the compound of Preparador 18Í! in Stop C, The compound ohiamed is convertid inio salí íbrm and íyophíiised ais deserlbed in the general procese ío obtain the i» ccibpndnd. iífyh'Fesüitfiítw ψί íirometrv (ESi/l·ÍA/fíJR β?η$ M$<S$>$):
Pmpoieai ibrmuia: C.;¡ H,n i- RA-h (M*HÍ euh nhaed'. 701 3346 (M4di| measured: 701.3282 fésaamie 422. Aí*M ly<lraxyphe»>-¡>' 1 ¿-éimeth&amp;M'il-mzthyl· I ^pyra*aM-yl)«5-C2» i\0S)^H^rvhmn^y^thyibX^úihydmkn^^nimf^^m^%mm^ 1 íAp> rralt-3*caiÉóxa»*ü*: bydrccfelcmíe rite tule eompound iv ohtajneU in 4 !»»»¥ &amp;&amp;Má of Preparar ion 35 and the compound of Preparado» f m Slap: A, φύ the compomil oí:
PrepiMiím !” la Slep O. The óbtáined1 is eoaóofled So salí Íoob and lyüpMIsed as deseribeá la iha geíteralproeess I» «Main the lilla oonapoaná,
Eiemswtsl mk'r&amp;emtfysh; {%> fheoretic&amp;hme&amp;s&amp;red}
%€····*? .00:67.4?; T»M^.05:S,S4; %6N12,34:12.46, %CX-S JÓ;4JS
Migh*Pé$éMtkm· mmsspseimmeÉrr (ESI+-/FL4/HR, ESI-/FÍA):
Idapmeai Idmnila; Cas 1 Un N<, f.C fM'SII'I' tnoasoAd: 645,3115
Eaamnk 423,5'a'54:'6n»ra"2"il;,CS4'-3'-ünari>6olan4'a4nial4vlV\5.,4-'dl6¥6nnaannsaolln··· 2( 1 ##"> -.y 1! £:¾¾ r Ι.ϊ í>Yiy i | |:.4uaty IpNlM'l-hydrnay |'4H:nyl)'dí.2'allnHaÍ\y6;?V''l I ^ysaaao3~y 0~ I 77" l>>aaa«í4''4~S'16'I7:6<|>yrrnla^3H:ar!M.i.sanHsle
The tiüe compoaad ss obañned la aceoniunce wdh ihe pro casa oí he;n'np:e s mmg ¡he acid of frepa?a?km 8 ana the emopound ni Prepararon 3' m Stop Λ, and -he componed oí Prepamion 2ét! in Síep C.
Eiemmíaí mh'rofmafyvh; {%> ihet>retimi:memureií} %068.17:6?,62, %i 1-5.66:5.07; %N-1 i 5)5:11.48
Higk-resoiimmt ..mms .\pi'c¿mmefty (ESJ/FiA/HR mui MS/MS); :1¾¾pírica! foimufe: (¾¾¾ Cíh<¡CT Íyad4]"pabülaíed: 7013205 |M-3ir ineasured: 705,351!?
Eianipie 424 AíT4-Hydro$vpli^yl>~fVT^infethaiypyriiiiÍdm-S»yi^U-tUiaaíhy8»S42~ | |{311“,3~í m«rphoIíri~4»y troethyO^^HlIhyílroísoqwíaoti»^ !&amp;f)-ylfesrtK>nyl| pkmy ΪΪ» I M~ py reokod ~ea riso % a m i de hyú mch lo role 73alitio eaínppund is ohtalpeá in ácotodanód saith iho proopsaasFEaprápie: t üslog the aeid nf Froparalion 25 apd tho componed of BreparadoP: 5! in Step: A, and lité cnrnpound of
Preparaunn U" m Stop t\ i he ^.eegxeind *Ttatoed *s eonrerted bao suh uem and lypphllked as describid tn the general procese to oblata the tltte compound.
Eiem^rdíd micromiídysis; (%, lheereikai;memared) 05'65.63; Td I -5.81:5.45; %N-1) 854 1,93; %Cl'-5.G0;4.9i
High^e'iúhtíkm mesa ^pecimmeky (ESi'-/FL ídlH, ESIVFJ.4E Fmpírk.4 i oírme a Ow 1 bk hk |Μ*ΗΓ euiculhkd, oM.M '1 r M U Γ uiee^oed ov * * ? >i E;S.BBlM.li2§$i^M^-<5¥ane-5-me4u>r;ypken>i)-5-f5-llit«§rx5''2-f|<3S)-3"fm<5rpFc!in"4-yimí4%'l>í3v4*dihydralso«|aia:»li»~2<ljsl>*yl!carhanyl)f»í**»yl)“Nr-C4~fe>'di'«xyphenylí''· k2-dñrr«llpv64,|#-pyrroirr\3o:arbírr.ar?kde Iwdroehlerkfe
The tule componed ís obuñneü m eceord&amp;nc;. wiib the procesa oí oxample ! using (he ackl of Prepararon 8 and the compopif pff^ptmísün arninp in oren C. The eompound ohtumed is con verted inte salí Ibón and lyophiiísed as desersbed in the gtrnerol ρηκο^ so oblato the tifie eomponnd
MkmenMl mmtmme^am fM¡ MeúmiEdkmmÉmmE %Ca-h?.2$:6(5.$$; kdí'5.51 >.28: -6.3'68.55 %tT=-4,7V.4.67 £MIBilliLJiÍu5-3S-Í:|;.rern~2-y<3x5>3-<mnrph.idin~4-ylnieíhyl>-'3^4"ílÍhyilr<nNnqrdnnikv· 2t I l|€arhotü> tjphetuylK'Mtd-'hyd^m phen> IV.\m»t«etii05i>'pyrtmkita»5'> dli»eífeyl»Í/f>pyrr»íe-3-€arbasamide Ϊ he di le eootpound k ohtained in accotdatsce with the process of Ex&amp;mpie 1 nshtglheneid o.f Prepararon 8 and the eompound of Prcp&amp;raiíon 3: m dtcp A, and the compownd of Freparat'son kP m Step C. iKSi ϊΊΑΊΪΗ t$mi MS S?A> i v no *al ou a 0 di d \ t\ jKts-Πi’ eaíeobued' 6Sil.30.iH i Mi-! 1)" measured: oOl,3060
Ex»m»lg ^IT^jVWS-^Cv^jKM^aacíhoiiVfthfeiivI^S^íS^SaorÍa-^-^HáV^-lmorpIt^U»^ ylmeíl\vÍ}"34~^¿%6TOfeáK|«líH>lia'2lII#)"yl|earl>ORyl|plu\iwIi''AOl4'Oij6r0«yp%e«v^'> ljHliisie$hyl-l#/~|.>yrr<>le-3-«8rl>6itmide byérneyorkle íhe tille eompound ín oblahied m ueeordanee v\iih ihe proeea* οΠΌηηρΙο I utñng rite add oí Preparación 8 and Ihe eompound of Prepamtlon 3! m Stop Λ, and íhe apptoprmíe omine ia Stqp C, The eompound .obiained is convenid inte salí iorm and lypphíüsed as descebes! i» the general proceas io obtuso tita dtk eojnpound,
Ekmeetfái mkrmmfym; {%, ikmM^ki^mmsmei§
34:f<ML T4 i ^ >1:5.3.\ *«N -TTT8.07 '"4T 4JT.4.SI
Ipcmpk 438, vano^lxl^lmgthyl-l^ftvrrfti^y fmorpholiu~4~y Inifctbyll^^-dibytlrobíaiuinolin-Sl I pyl|cpii>n»yl)pbenyI)«iV~{4<· hyilroxy pheny |)*| ,2''diníeílyyÍdlT|yyrrnl&amp;-3'-earÍ>o^sodde hyd roedlo rule íhe tille eompound ís oblo oseo in nteonlauco wish úse process oí hxanspie 1 sssñrsg Ihe uod oí frep&amp;ration 26 and tía eompound oí hopa,mimo 3' in S'tep Λ. and che eompound; Éh Prepandinn 1IW in biep €. The eompound obüúned ¿s converíed loto fifí torstt apd iyophiiked <¡ * deso shed os km geno?al pro* o-ts ro ehguo ihe míe .vmpouud.
Ktememtii wuromah ue i V ihemv measutedl °cO 66 Όι,ηΟΤΜί ' 74* ..\ U40 1L43d'4'í 4 SU;J *»* tíigibresoiumm num spe&amp;romwy {B8i+FFiA/HRs ESF/FÍ4)♦·
\ ¡«1' ! i H'v I lj í \ P j&amp;T íl| eaieubted; ?ü 1.3246 |Mvííj moaNOvcd: 701.3243 ||(3iS'^^'(m?ifr|>luíllii"4'}Ílm«lhyÍ)''3;'4'dihydmk<>qiyíHílh'S"2(Í#l}''yÍ¡s.<arl30«yl^|>h€Swl)'^ ftydrocMemle
The: title eomponnd Is obtained in accoidanee with the procesa oíKxampie 1 nsing ihe aekl of Preparación 27 and che compound of Preparados 3* m Slcp Á, and thü eompoimd of Preparación IT1 in Síep G\ The eomjx>nnd obddeed ie convertid tino salt foro* and lydpMliM4ai':dtinirflie4: ftí the general procesa to obtam the tille eornpouml
Efáííieníat ttiicraenatysis; {%, iheon'tícú3raen%nred) %íM5;JMT43' %1T3.?TS3?; 3331035:tilJly%0M32:4 34
MigM-remMimn nm h* spevhmmin· (ESl/fi i/HM a mi M$/M$¡t ESiAFlA);
Brnpirica) formula: H,.;;< V Ni, (T j'M-ΗΗΓ edsmkted: 7313352 ' measured: 7313351
Ikaoiple 4313 S3S-Ch loro-2 -113-(iriíiaoromeihy 1 )-3,4-3thy4radon» inoido 2(lH}~yI!" carfeeoyltpIicnylP'AM^43*y3ro&amp;yphenytH, ^-ílnnefhyí-íV-í ϊ pyrklí n-5 ' y i}'· 1 H -$>yrroSe-3-carfeoxatnide l'he tille eompound 3 ohialned in accordepce dáihthe prpééss oíExanipIe 1 ksipg llie aéid of Preparatkm I and racemic 3~«trstluo«uncíhy iM 3 3 3•leirahydn.nsoqutftoí ine ¡n Slep Λ, and ihe eompound of Prepararon 1Γ* mStép C. H'tgh-resaíufwn mas* .ysecírometry (ESiWFM/HM mé MSMMt ESE/PlA): lonfñtichi IdfrodiT €3.1¾ Ti 1¾ 33 %. |M3M|" ealéulMeiP 6283147 fM“H4|' Mesataesl: 6933144
ExMTOk 43 Ix S-iS-Chlnra-l- | “{m« r ph o1m»4«y inwtfpv !}'3 ! hy á raisocj nina 1m~ 2{lü)"yl]«srfeím>:l|piseüyÍ|"A“{1>,'5*^l^^yMiEí~|J):^s®oM^Í>».?V;»í4“hy«lr^x>'phí:i4ylj« 1,1 -'íibsielhyi-1 H~px frolfc^-^srboxaiaide feyiiraddnrkk
Eiemeniní mk-roMwh'sh; f%, tke&amp;reth'iiL'measiirgrfl· %¡V~ b4.19:64.37; %H-5J0;>.18; %N-UJ2;I i.55; %Cr-4J6;4J«
High-resoimim mms spevimmmr (ESh~'Fl4/H.R! ESI'/F14);
KmpIHgjtíi &amp;nTUílif (¾3½ O MjvCk jM+Hf calcúlate! 493.24$I |M-Hj“ ommmmL 443.2452 ifatnrnnia 4^2»545-ChIor«-2-ISc3i.yKl4mftrfífe8li«~4~vlmethvÍV3.14^li'bv<i.r<&amp;lxi>««iM¡iü^ 2(1 Hy^mthmnl} phersylpAM 1 íS»dlmeihyt«lí/«|>yrs«»M'‘>'lHV’»C^f«lrex>|íheeyÍ)h ljf2“dÍr»ísíhyS“'l/#-pyrrok>'‘3-«srbo'ss»íitÍe Imiriadsferkfc·
Btemetif&amp;i mictoinmh'U^ (%, theútefimi;meiisiin'ii} %€:::64.19:64.43; %Ϊ-Ν5.80:5,22; %ΤΨ==11,52:11,60; %€Γ-4.86;4.66
Hsgh-remitHüw ><mm .φ«1«ί/Μίΐ! (ΕΒΙ^/ΒΙ 4/ΉΗ, t'SÍ~/flA):
Empinad E manda: C?!, EL; OEUCL [ΜΉΊ.Γ calcubued: 693.2951 fM+Hf' nlbsnondl: 6933953 comppusids of Imamples: 431 ¿nid 432 amobtninoá in nceordssnn witli -ihe prneess oí Ennrnjnn | psmg. tibe ankt oO^paoAinn } and t;h© coKvpopííO oí íbnpaxaónn 3: |n Stop A,. and Ole mixture nfídeparauon 2?!: in Stop €. Al Ihe end oí Siep D: d-e nnunem are nsgainied 6y pnípápatlv© 1IPLC; uaing sneinnibalp and wutñmn'Á 49 nlnanta. Albir svaporaihig olT íhe nolvnrM and neutmüáirip wibh soáibni Mnaríbonaim bui pmdunís am snbjepted tn a snsp nf eonvgjninu bbo nüt fmm in ihn pmaence ai IM. HO M otilar. Afte? flfipi'irm and Ipjplbllsbtton 'i$á a mixture ep :;|^gimitrlln/wa|ssr:: ihe tlite producís are ebubned S¿$ISII|hx«XAMS4:>®8#>«$-melíi>d4.i^m>k3~>d_HAM4”hydníxyplui$yl>~lv3·'· yl|c»rfeíj»y! |pbeny ¡p1 IApyrrole-3' earbammide bptroe y nrMss Thenitfe comixmod is obtained in aeyprdanee wiíh the procesa oilkampk i «smg the acki oí Preparaiion 25 and ihe eompoimd oí Preparation 3: in Siep A, and the oompoural of Preparaiion 19" in Sien i.\ ('be eompound obtained Ls converied imo sah ibón and lyopbitist'b as describid in the genera! procesa to ohtain the inte compound.
WgmgMitt mkmmmfysis? (%> tkmrétk&amp;kmmmmé} %ί>:60.12:68.29; %H-186:5.40; %N-11,92:12.05: %CT-5,03:4,92
Migé^mskÍMiáti m&amp;m 0$M^ÍA/MBMné MB&amp;fS)í
Empírica! lomuda: Pbobk 00 |M*llj Acúlate j po9 dd! [M s i !|' rm.asored: 669,3184 lAMilií2Ílit-'d.94<li?nm-2"i|<3ál'x1dmnrplo>iÍíiA-aln:elbYÍp3A~d$kT<l^n$e<|innnlisi* 2(1lí)-ylÍiniri)o«yi}pbenylixSH4"h¥clra^ypbinyvl)A;2x1inHdbyl>A;r"'(l'-nietbyP'2J'' d ibydrte I #l~py riadaf2,d 6fpyri d m~5~¥ 1)-4 IA pywde~3-earba^ amide ipyd rneW cride íhe tille compon od is obtained i« occordanee wnh the proeess dcsedhed in Step B ni' i \<nnp:e 49 o\tng i vunpk- 'S' a'-, aunnig inakiUt Πκ exmpoimd An.un. d is cojoemd inte a ai: ;orm <md ÍUiphiioad na described in lite ye no ral procer te obiairs the dile ccanpnundiü
Fiemenmi micmanaív>is: fV thi’otmaihmemtirifdi A< o ’ iSp'K 03 ΆΗ 39ÜÑN0 *A * ;.|9 10 yQ · Λ1! 4,"A5Í 4»yÍMtí^yi|'<^4*dibydr»i&amp;<>í|i?i^«|l«-I{||.|)~yl|tar!>«'«5¥l||>fe^u^yl>AH4*l¥íÍrtiiypb^^3';!h I JHlimafliykl l#»|wrr^k^3-€arfe«ssmMk hydraeypádÉe i oe tille compound is ohlained i» aeeordarase wíth ihe process of fíxarnpie I usingJhe acid o* Γ?*?ρ t ai on i4 aad ihe eompouad oí PreimraiuM λ ¡o Mep V and úse appropnate arnsoe ít) diep i' Toe «.ompound ohisíncd is vonverted. mí® salí íomi and lyophiHsed as described ¿n ihe panera! procesa io obíain Úse sitie eompoutuf. íiigh-reuiíntim mas* spectrometiy i'mpíTíCiíl formula: C!5 H * 1: ¡ [M-r f!|" eaieulaied ? i 5. }03O | M * H j" -oeasured: 715 3040
Esdiiple 4.36, S~P*ÉI®»r»*2 -11 {3^3^|marpk#ite-4»yteethy l)^3^ái^drnlM4ai®»fc 2illdb>d|estrÍ;sü»yI)plHsstyipeV~{5»eyaneilús>phes^2-yik.A?v.|;4dtYdrnsypbenyipL2-ííít«eíhyk!#/-pyrroÍe».Vcarl>®sanüd« h^droefeforíde j lie tule eompound ís obíasnea i.o aeeordsmee with ihe procesa of f'ix&amp;mple I usiog ihe aeíd of PsepnKOion I and Úse componed: oí Prepausúon 3' in bíep A, and ihe appfopriaie amine in Step C. 1 he componnd oNained is essnveríed soto salí torro and lyophilbed sis describes! Iplbrgdidral prc^ess so ohíain ihe tille compaund. l 'h?.(m.nhii mk-roiwafysk: {%, ikeúrBimímtmmfí^i <042-63.07:63.09. 7<sH-5.02.4.?ih %N“«í .43:9.35; %S-4.J2:4.0O; %C!-4,??;4.$0
Bigk'-resüimimt mm$ speettmnetíy (ESI/FIA/JIR ami ñ:lSMÍSt ES/WF/.d.C'
Empine»! formula: O^lfe C! !4|d4ii iMHlf oakoiaíed: 706,2240: |?U'in Ok\s:vavd 706 3250 "Tile íitlfe ©&amp;mpHi«a -m 0fetá«íéÍ4». aeeordanae ^lli:t|i;praeess of &amp;8tpp!e l::|||| the aoíd oí Preparution 8 and ihe eompoitud oi'Preparatioa .1' in Step Á, and fí«e appmprtate amin*· in stop L\ 1 he compnund oofained ús conceded mío sah tona and iyojphijieed as describid i;} fía: genera! poxess 10 ohudn the uüe componed.
Mgk*mimÍM¿mn mm$
Ihnpsrical formula: Qd ír?l:jK#i: uM-iíir eak\¡íak'd' \k.:th>4 [Mh-H]' meas·.ired; 7D2.2B8P :Wmmnh:4tH* $«(5«€h lo re-2 - f |0$>-3d m o peiího 2í í fpyifearhooyT p Imny Íp/V~t 4 ~ hv d royy p heny 1}-1 ;2»din^fbyk/V4|>yra«in-2~ylblH-P3 reo í e- 3 - e a r h as arn Id e hyd rae is lo π de
The tille compilad h obiained m necordance wiih the prncess oí Hx ampie ! usíng the acid oí í^etwanoo i mo the ornaos re ot'Pm ' af xa1 h m Vep \ \ad iho atppropriate anime ín líep CL The eonipoundíobíained is eonveried M# sáíí f&amp;m\ and lyophlllsed-m desoábed: in the genera) proteo u> ohtaín the büe campo-md. fh'mmhii mh nmntiiym; /V thü'ttrstk'aL'imtusutt'dí 1 » e3,dStid ^ , '\d s Jf? s ‘ \·«\- t ?5s U6L ( ΠΜΟ
Migk-resúiufhw mm:s spectrometry B$$~/Í,Mj:
Bmpiricai formula; í\,; i t3, Ci Ny Ü.} iM’HI'exih'ultf d. h'\i"nd8 :Μ-ΜΓ mommrod'o"\:nT> $ l}* 1 tjbdlmethy l~ 1 ^«py hyduHiiiteíIilfe
The tule compo und ia obanued m aeoerdanee witlt Hie proeess oí oxampie i usmg die acal oí IkeparaUod ti ail l» eomponnd oí Prepararon 3’ Id Síop A* asÉihe componed ot Preñar uíion 12" m Step Ch 4:he; componed ofetó**Nt :*£ dio S#ll loop ind
Iyophi|ise5.1 üs desorihed m bre general procer lo nluam thc ulls eopipxibb Hígh-re&amp;üí&amp;fhm tSfkPfA):
Empinviú formula: C.¡;! Hs¡ 1' Nk Oí( [M-t-H]'crtlcuiatód: 721.3144 fM-fH f iMtasurcd: 721.3144 fonionie 44ft> mM:mm4 Jhf ha&amp;feyM rroP3-yIkS-<^inaro^in^lÍmaFl~ A4(4~hyd rnsypl^uyl;i4k'2-ciuMrliyI-í/#-|wrrale-3-«arb»saniicie kyctroeiilnrlá^
Yh,; tule eomjwmd o obunnen m nec-anume unís the procesa otTxanspk; I nong íhe acal esf Prepaiadon 14 aod the compouml of Preparallon 3! m Stép A, and tire epinponná oí P ropa radon I K<: Id Slep C, The coorpound obtamed fá couvertel ¡rúo $a!t íonn and lyophühed na ®PíIthgd4o íhe genera!: proaibi Mofetgid thplltle opmi^ppd, M0rnéM0Ímkmtímijrm: {%Jke®?eí&amp;8tmm$wm$ %(O-6S.75:653>h:%I I-k.7K:5.50; %N-i 0.05:10.47; %CÍ-4.62:4.42 ΒιΜψέΜΜΙΜέψ 0SÍ^MMi, MMklJ?|b linpIrtPhllorliila; C,? EI43. f :¾ 0S:: (Mt-H|' calcúlate*!: 73!,3352 (MM1|! ureasured: 731.3353 2( I IlTyif e&amp;rbotiy i| p betiy Í)~k4S uy ao o -1 US <t ímet byi- I fl~pv mxlub-y i P 1,2«sli«íelliy WV» pb8ByÍ~!lí*pyrr0l«»3^»rbo&amp;a»tsde hydtmyorkfe t sale eoospesstsd ss obi osa d sí? a., esoanoe o oh ?k. pu^t u oí l· xaospie ( tóese the >sesd oí tAparaisots 1 and ibo uns^vone d iUowasov ' es Meo V os A día eonponnd *Ί dreparalion 2'*" m Step €, i! heisst: ondesosood dsat Stop i> is uoí eatrkd otst. The coropound obsasned ís eonvetted ίαιο uds form and iyophibses; os? desenbed in üx? general procesa k> obíain Use tille eotopossnd.
Ekmemai mkrmw&amp;fysis! í%, íheoreHciE^memureé} \>C'su 'nmní ! ,H - M'-STTA' Π tdMUVA" 4tK\ a 43
Htgh~n>$&amp;itttíen mas\ npecttoittHty /íSVI 0¾¾ ESí^LE};
Huptueal lomuda: C5J H5Í €s Né ()¾ [M-H-if caleulatetl: 7fE3« tMHlf meatesred: OtlJoog 2{1ií)»yl|e8rbo»yl|piHSíiyi)'-^SxcySjH3tldophe«A~yl>AM4"lwdr»sypIwtp¥l)~!í2~ dlmethyl-l lí»pyrroÍe-3-e»rfHHE8n*ldte iwlnsehioride
The title compmmd is ohtaineá m aceordouee vvitfs the process oí Bxampk I usñig the add oí psepiootion 1 satd ttsr ooopossad oí Onponstism T ns Stop Λ, osssl íhe uiopxxmi oí dsx'í'os,os^n 4T* iss Step C. Vbe eosopoustd uNasoed ss es'nverted imo saüt tora? «»d lyophsiised as dcscribed in the general procese to obtuso Use sitie eompound. i /tuoí-on¿/ m¡crmma(rsk; fT, EtetmHh'(ikwmmmÍ) %H -=5.02oU?; %N**M3A4 \: ^4.)2:4.24: %cr~‘4.??;4.5?
Mtgk^&amp;SéMtEm: mmsxpestrmmtff (ESE/EEf EEE/LM}: b.mpsricai kmnula: C-}9 lí^Cl s%í%§ (M -H'f eakas Usted: 706,2249 t.M-'Bf ntesssured: 706,2252 luán»»!» 443« fpToloP#*élpys1#sJf~yl|»A^ q^W(tfm~2ttfthyϊf<rfth<my!ϊphmyil·f,?'iiϊtitefiir^i'S\(f„met|iil~jβψ]ir?βfofJJ~hjpyt'i^iw* ÍM tille edm;pb?aad is ©üa¡»®álfe amsrianef m4ife?|^;pr^^:;0fi|^8t«^ | |§1eps A loCi) pÉpg tbe ae¡! gjf Preparador? 28 and ?He «eaifggjwtó |>íffgra|liP ψ m Stop A, pighe compound ni'Prepare?son 30" in Step i
Sm B; 5^i!1u<>ro-4^y<lr^y-'l»||fXSli~3~{m<>rpb0ÍI«"4-y!iSÍeíbfi)'3,4~tlíSiyártsiss>«· d|pyridi»-$*yl>«M>phe»yÍ'*Í^pf'**iN«3^^ÍÍíÉlli;
To a solutkm of ?he coropepii §f §?ep Á i j g· 1J? niMolií |ü Mfeplretay É0llÍ<>Mípe?!»i VH* mi ? tlieu l· tdded< dtnpvxke a? 0"V< a IM sokukm of barón iríbronikic ín dicbloromethaue O 8 mU 1.8 nano!). Ate;stírring toe IS jpors a? amblen? tempern?mp, ?he rcaction mixture i a poured dropteise ir??© a aoksibn pllf af -«1IFC. Ate sürriag for one huur* sautrated aqueous srodium bicarho?*aíe soíution is adcfeik and ?he reaciinn mixture is extmcied χχ-uh «Jiehloromethane. Aíierdrying over MgSOi. ilp reaidue is punte! on a aldea peí eoknnn usmg a mixture of dkldnroineltprne mú methanol M ornan? to sield ihe expected nrodue? mms speefmtfmi^, '($$$$$$&amp; MMMÍMM·
Pmpirieai formula: ij H;. Γ \s i O |M H lf ealm iated: 713.3 246 jMHIf' meaNtuod; 113,32 4 4
Esamote 444 $«{5~0*Ιογο4«Ί | 2íI#-#Kvi!eartioi>yl||íhepyl)-Aí-H-h>'ílr«xy|>Si«»yl)~A?-<S-mftthoxy|iyra*m“2-'yl>'l,2'' di nudhy i»III-pyrr nk-3-assr hesate# hydsroehiaFlde
The Utle compourtd h nhiutned m aceordanee wUh te procesa oCEnample I «eing te add oí Preparaban ; and ihe sompeand el Freparaíkm 3' m Stop As and te :gnm¡príass a ni i as in Stop C. The eompound obíamed ss eonverted inío salt forro and lyophiHsed as deseribed in fhe genera! procese to obten íhe tule componed.
Eieme nmi mkroimaiy.m; {%, fhem'eíit'titmtfmured) %í>62.te:62.?2; $#1-1,42:1:245 %lte1130: i lte: %<T-4./7:4.62
Hiph-resehpüm mms specírúmein1 (ESfr/HÑ, &amp;SÍ-/LH}.; EórpMcá! íorMdld: 'C··^ 11^ Q 66,47$. ί M -11# calcuiafed: 707.2743 jM+Hj’ mensural: 707.2747
Ii2IlM>Íl3Í4#73'54;idarii''2'||6ÍSV\$'44«or|i!«0!a-'4-'y!o:H/dPsl}s3si'di!pvdraiso<|aiaid6i" 2{!ff)''Ssl)car6/asy!3>fe/apví6A'44#>dros>p>bimyl6l3'dim€dia!»47| k (indityíerkpnelhy I)#3'di!iydrft" I &amp;|>ym>Io|23~é |pv r!dio-S'>i|-! #l"pyrí4il«alÉ c a rtm x a m ?ri e h yd re eh lorid e
Sfep.....A: M^$*C%foro-2-ft{3S)~3^mrphoí¡H-4~ylmethyi)~3,4~dihy<ir&amp;iii0qumtíím«2{l.f{}~ yífeurbüiiyífpküttyi)~N~{4-kydr&amp;xyphmyf}~If2"ittinethy¿-N~j[l~{irídeiiterwmgfhy¿}·-/17-pyrra 0//2,3 ~í>¡pyridi n - 5~yí/-3 H -pytrok-S - m rboxsmide
The intermedíate ts obtened tn uccordance wdh lite proéMS oí Exaropie ! usirtg ifed aafd oí l^repárélién i and ite aomponnd. éí teparahon 3S in Blep A, and te coropoamt oí Prp|íar8tlon 3 V‘ |n Stop €>
Sm> M: $-($~€híúrú-2"ff0S)»3»{mú¥phúUn~4~}>Ímeíh$i}~3t4-díikyiír0Í$oqtiWúi$tt~2(iH)·' yi^arbim^iíphetiyíh^-^hyiifitxyphetiyíhít^im^k^i-^ <Ííhydm^Jtif-pyntiiapj'>b^yriáim$^yí¡-Íff'itymík^^éarhtíxami(ie,hy(il,Mkíomie TjNf-:fro#^.^' ;||r'te::aemr%n«e: .Λ· tlur protoeol #príhai m Síep R oí Example 49. it: being ündersuxxi thai the produci h then xiibjeeted So a xtep oí conversión m?o saíi fotm m Ote pÍeevnee oí l.\f rhVt in eShet. \f>os ÍHuatam and hophihaanon in a mixune m ae^onOde oo:e tK"\h <·> <.\K * n-, umod
MiemmtíM (%, theer&amp;kukmms meé) %í>65.45:65.33; %IR$.?8:5.S9; %N-10,90:10.82; »40-4.40:4,28 íiigh~res&amp;fiítion nm\.\ spectmmeín {ESl·1· iiR, ESE i E);
Empírica! formula: €,ι; Η.κϊ Ci 0¾ Ní; 04 ¡M ñfV calcnlateÉ 734.3295 [M+Hj* measured; 734.3390 ,g^P^lü&amp;iNS~Chlorio*2»íf(3á->3*Craonph<ilin»4«'yl»netliyIi«3>4»díhy<lr«»oq»l»«lle* 2{ I //}~yil«:arb«ayll p h my 1}4V~{ 5 41 o ara |>y raxia-2 ~y!)~iV;' (4 4sy 4 ro xvp heu.y i1 „2-d ? meíliy 1« 1 $hpy r rnlo.Vea rboia mide hyd m e h ioride
The tale eom;vund s\ mnuiued w „u.coí dones v, r.h the procer oí 1 \ampk I u*dm> de .«nd :df Iripamida 1 and the cíhpphháidfPrepasmien 3' in Step A. and the appropnau: amóte ¿n Sh;p C, The componed obtaíned iseomerted inío sah fono and lyophíliaed aa desenhed n ‘7 ϊ. ^ ^ T p vH e \ obtain *he M* \< 'upo md
Fie mema i mhraumaíy^h; (%, ihemetieaEmeiisareé} %C-62.28:62.16; í-tdi-SJ0:4.97; %N«! 1.04:11.35; %Cr-4.85:4.4Ü
High-n 'íohíH<m mms specteometry (ESif/ílE. ESE/LE): hsnphiud formula: t\N H^Cl FMí;0.; ' Π}' v.doulated' o^5 354' fMtl{)+ meaeured: 53)5,2545 i he üHo compota td te ohtamed i« aceordañee wilhihe procesa of Bxample 1 uslng the add ni Acpmaum 2o and tho νΛ>;ιχ\>'me νΤ IVp andón * 1η Smp Λ, and ηχ> compon'5 i oí i7ep»raiíon Γ *n Step C, H»o componed obuñneO i'.' comeried mto coh U>r«t and hophnkod a\ dcMnlbed m Ha· general píocovs ío ebtain theíñle componed. WMíwhímí mienmntiysis: {%, fhwn'ticabmemttmd)··· %(>65.28:í>5.2?; %H-5.77:5.51: <999-12.02:1.90; na-$.07;4.?4
Mi^^msáíufmk mm$ ^peMrmmírf {ESif/íiRf ESí^/lM^
Fmpsrkul forimiís·: Cu fí,* P N,. <% l-VíHU" ealctdaled: 663.5091: fkle-Hf MdMdrMP 663.3014
The titie eornpnani Ixnbtalnííd In ácdarrknae wl#:pg pme®p oi-iiappk 1 nspi llpddíd of PrepíuaOon I and thc componed of Prepararon 3* in Stop As and ihe oompound of Preparadon 32" m Stop C. The- cPinprund óhtaíned te coimnted ijtu* Mil ;fci 'ttvd '' onhdmcd o,c dcacíshed m du general proces» to obu>u the ritió oompomtd* BMminiM mk^enú^s^: theoretieahmemureil]l· 'Ft p4 m-Ό 1 5 n Ad i 5 -15'> 4'6 'F.K I 1 '2 U Ib.'06 1 1 7 3 36 /íl}>h-r <o f6mio^í mtns speefrmteíry (Ι7Λ7 VW76 6'Λ7~ 7 8j>; IVoph'ioal ñ^nuno 1 H<:. í i 16 H ^9 jM * if tolcel Ued' “Ob>59^ [M: Hj mensured'706.3985 Γλ anmk 449< SdS43hkm>I·· ¡ Ü&amp;fikM m*jn>httlíB-4-y li»eife.ylK^«4^Íhy<lrolsftq»iiioIi««> v¡|„:^4.^y1Ím.SvplH?«yÍ}4;2Htimelfev^4ff-pvrr^k-3^sr!K>.s®Mkk h;yir#e!iferi# Ππ; úile cnmpound js ohi&amp;ined irs accnrdance witb íhe procesa o! !:mample 1 using i he aeid <d Frepanmon | and the eompound oí Prepnmtion 3' in Siep A. and the eompmmd of Prnpamimn *<*' Siep t - i he compwmd obmíned k eonvtriod mío salt Ιαπη and hoplrflku! as dos* Hia-d ¡n the penara! procer ;o obutn the tdk eomponnd MWmé&amp;Mígmwr&amp;8nnfy$&amp;g f‘’»> ^hem0m0ímem^w$ %í>65.0?:64.55; %H-5.62:5.S!; kN- Mil :U).9S; %€Γ-4.6Η:4.58 liigií^itiúíumm mms specirmmín (£$$*/&amp;&amp;* ES$~/LR¿;
Empinen! formula, i.4. 1Κνλ 1,1 13¾ Nn οά }Μ I í\! eakídaSed: 730.3! 39 fM*f!f mensurad; 720.3143 £:sampk 4S§<Sd542ldnjxr>2-l|d3S3-v34nrnrpiraÍln^%damtÍnlk3.k-dldy<lrah'nrmdnjtiño litifro if emrkmvi| pimovIK^Cd-ei' ma-! mudlo l'lil-pymrnl'S'ylf'íVdd'·· irv d roxypb raw i l -152a1imeiiiy1' I H~py r ral a-3 -en rOn &amp; a mida hydraehlerkk ihe n u ^ >o '' td‘s a\ nd i ,v(,¡}, <.e x*.ah the οιοοοόm | vuupU ! ev¡my no <uo oíIkepumdon l and ihe vompvund al kepmauon 3' m Siep A. and the componed oí
Fuá ¡ton 5 ' Skp v K' *a opmu ! y mime*. \ >. mc'kd mío salí íómt and lyophüised as described in she genera! proeess ío obíaln ihe tille componed.
Sientenfat mk'romtaiyshi f% the*treé'xknmmtreé) %i>63.24:62.62; %l KS.31;5.09; %N* 13.24:1.3.04; ¡kí'i'M.79:4,37
Mmk-rewímiútt (ESMW* ESI^ÍMM
Empines! formula; C}t> HÍJS Cl N, i^ |M > ü| y aicutnfed' 704.2744 Imensurad' 704.2747
Blaifti»le4Sl4 f|f3bS)4H morpholItt^-yimctliyl^^ihyilrolsííqittieoHwi- 2(1 #i)~yl|e&amp;rbe»yt) pfe^y i|»A^4*Í*ydroxy phe&amp;y t)~1 ?2“áÍ.í»ethyl"AM I -smThyF til-|íyrmoÍof3*44^pyridi»^ylH^”Pyrroi^3^»rb«ss;afiii<te hytlracliteríde
The hde oompoumf -ts >oím < oí ,u tou\\ h *L· o uu vs ofí \ mp o!>' ut 5 < *\i oí' FreparaíKin 8 and üv soinprniíid of Preparación 3* in Sfep A, and the eompound of Fk emane I ! it‘ Mor O ík *. mmoaed ohl * ** κ n. ornead oe vih a v m 1 lyophilised as deacrihed m ihe general procreas lo ohsain fhe tule componed. (%, théámíieélimmsured} %<>6SM:?>é2%; %!!-5 >1;5.4S; %N-13.07: i 2. i? %C1 -l.?;>:.\5i
Exaamk 452-, /VA I J~Í>ñmdhvM II - η ymm M~ v 1 >~S4STleoro-l"I|(3<S>-3-1marphaIÍn-4~ yírnenttyrud!s4'Ul¡hydraisríptiifi<disn'2ri##F^i)e8rhenylÍph«y!TAf'44''kyiirímyphe»yl)'· TJ'áiixetkyiTiTtyyrrele-d-earhesainldirfeydrnelderide i''íemenfiií mkromafym,' (% fhe&amp;n*k(iÍmMmW$h %C>-65.68:64.7'?; %H-5.44;S,5S; %N-J 1.78:i0.69 %€)-4.97:6.48 opá E&amp;nnmk 453, AHI,$"DimesUiyFifí-pyimoM-yl^-fS-Íluer«Fl^(|(M>3-(i8e.rphoÍÍd4-ylo.KdliylKM'Ufdn drm§mpinííÍio~2C 1 77í~5 IjcarÍMmy 0ph<f«> IV-AHd-bj ür«xy plienylF l»2~<1iai«íhyi~lil-f>yrr«k>»3-earfeí}xuí«ide hydruehlurhJe -MkwmM#l ítoiettiiÍtÍwiysfs; {%, tkeoretimknwmur&amp;í) 66(:-65.68:65.4 6 1514-5.04:5.62: %N-t! 78:?U.93 4,,0--4.07:5..60 1 he eompoumls of Fxamples 457 and 455 are ohamied m aecordonee \v*th the procees oí 1.'samóle I usjuií: 1BéÍiM:PF:i#piflílon 8 and Ote eooipihtóoí fepliadoh 3! íitilep A> fmi the númre of IVenarahoo 27" ha Sicp C. Al the eod of Stop O, (he isoaiees are ^panoed hy psepounuc IIP! Γ ;¡áioe -scetonnuie and waíer-TFA as e huirás, Ador <vjp<rdu olí he vlu í 1 * a aOup adh o<í üí ' > ο>οΉοϊ \ * i pn dor^ , ae íí1 |í> %© ps^ss©rip« oí 1Μ ΗΠ ín eün;r. Aíicr tiUí.Mion and hnpÍbii?ahon ín a nh\nne oí iiv.vto»i(ríkvw«wr. üie hüo pteene^ -r*. ohiainect ! ,3-oikn «i h y 1*1 íl- f >y r r o leO-a* r 1«? saim kk
The 'ido uompound >s obhnned its aeconlo^e o oh ihc |irA\v o* i x oh pie 1 o.\ing do? acid íram Prep;.m.mon ! and racemic Htriíluoromethyi>·.{^ \4 ^clrahydcois^Mlfttóte? Id .Mor \, ¿ni* Ju. vjvíjVU d o* P>t.|uuibnr 1 s Mtn*
Mhmmi&amp;i mkroúnafym: {%, ikeoretkühmemureé^ oO ft4.?í'.>4k Mi 45\45:\%N líOWoO
High~H'\ehikm m<t*\ ψ**< inmu‘?ry (MMmMMjí nnpu'iea! VohO 5 i\- l!> *1 h* hk 0-¾ iM *TI ok'H Oí d oKoíMO |ΊΜ*ΗΓmeasured; 686,2145 e»HK»ayl}phc»ylh^{5^y»n<^2<met)^!H2Hittorpitoi1a*4^Í)«Hiyl|*Íl?>i>ym»Í-3->'t}> .AM4~hydroxyphe»yi)4,2«tlimethyl«lÍ?-pyrmle-3*e»rbox*3ÉÍI«iÍi^ÍIMlr:tóáÍ!fe, Ί he tdk- compound ih obiuinud ín uccoixtance wíth ihe proeess of IkiiÉpIe I ρ$ΐί|| &amp; sdtd of Pr-eparuhou i ;md ihe íaenprn-nd of Prepnraüon V in Step Λ:ίand; fc ooínpwnd of Prepandon 35<: in Mep O. The eonipouml obiained is comerted Imo s&amp;U forro ápd hopiolneJ a-' doaenhed sn sfo genera! pn'uov·. ¡,- fonaín the uíe compound fíiemenM rntermmaiysist f% tfieeretitáfcmemured) %Oñ5.71:66.0?; %Hh5.?&amp;5,82: %N===!0»3;10.f>íi; %CT --4.02:4,45
High-resniuthm mms (EBIeSíM; MÉI~/LMp l'mpkic.O fornufor r¡J ?,5 CS V« h j'M-Hij’ eaíeulated: 731.310:7 1M'H| ivo<ssuk0 '"ίϊ ,»109 lllSSillSlí $-íS-€í$hr«-a"iÍ<3Í)^^ie$h> l^^dihydmhequiií^m-af I Hhyfy csrb«a>01|>fe«»>4)“íV~{4'-hytlrexyphcsiyÍ)"l»Ml^feyl*íV“(a*P»{ii:u>rplmlfe»4~yl}'' 1 he thk emopennd i5. tdnumed m uceo*dance wuh tíitr pomeoN t»f p vample 1 tudnp fu; ¡ul ·"#· Pf^üStíóB· 1 án#dfe&amp; dompOiisá'rf m Step :4, and the eomp3iind df
Preprnafion 36'* m Step C. The cwnpound ofeudned. is convenid mío sed! fono and lyophilked as deserihed lo ihe general procer 10 oht&amp;m thp |IOo pon-3.po:í|od>
Eh'mmfui mk nmutfysk; i%, thearetimknmmtmi) %C-63.41:63.51; %Hh5.59:5.26; %N-11.00:1 1.10; %€Γ-4,6Ε;4.46 tiigh-resoiutioa mm% %i^címmstty {ESI^/Mñ, ÉSIVLMl: l'mpiciea! formula; fo,.5 H,s Π Nfe O. |MMl|\:afoulated; 721,7900 M ü|'measured .1790'
ElM3iÉlil2¿5'(S^Cb$or«»2'di<lf)^4mo$iih<ili$v4"ylmeíhyt}»34«dlhydreÉi#i|i$Í$ínlhí* 2CI//py1¡eurhuípvl|pt!!eoyÍ)-A'«|dH;¥»oo-S"m«llioxypyrk!fo-2-ylVAO|4-h¥<lrí^wpheo>d)" l*2»ilÍmetliyl*IÍ?*|>yrrole-3«carbox»mMe hytlrochloride 1 he nOo eompotmd ie oWume<im «sordanee wsth dio p?oeo\e <0 l\ampV S osiop too oetd «O PtcpusuOoti 1 and ;he eeanpmsed <» Sfoíonomm m Mep A, and the uppmpdafe ens'Ui ?u htop Γ. The compottnd obuim-d te omnormd mto ?,df form and lyopkiUsed a-·: deseribed 3n Ifeu goneiBl prope§§:fo ohMIn Ihe Ode oampopodi 1 EkkmimÍmimmk!^lkE{% thmreíkukmmsumf} iSA>h4 l ' r»T*>5: fodl >.' v4 7*3 n\M0,9' SO 97; foCi" 4 62:4 22
Higk~reM*iW(*m mus* tpeammttry
Km pírica i íonouU C.p· ! U O U 0¾ [M'i-Bf cúuUU; 731J743 mcayoied: 73! ,274b
Sil!EMlLÍSiÍí34S'Cl$h)mx3'>y|3Sj'-3-|m<H'phti!ir$'4rylB>eth;ylí-3>4-dy)ydrah<á^«!iie!ii:i'' 'l{l/l}~>i|ia5rbo:nyI!p!Haíy!.h37'í5''í'ysTH^i,4-dime^li:yl''17l'Pprr(s!''3''yl}'vV'-{4'· hydimxyphfetsyl)»I ^»ámieíbyl·l H-pyrmic-3«earfeo x amidc bydrocidorkk
The íiiie cosrspouod is obt asneo m aeu>rdaiam Uín ihc ριαοοηηοί i:\arople ! usina ihc acid oí Prepararon ! and tile componed oí Pmpamtkm 3' m Step A, and i he eompnund oí
Preparaiiun 37" in Sícp (.' ihc eompoaníí obtioued ts eonverted Hito sai? íbmi and iyophibsed as descrihed in ihe acocori pmeess í« ubiain ihe tille compuund.
Eiemetiiíii mivmeneiym; t%* ihewefkahmem'^mi) %OU5.34;65,ó8; %IUS J2:5J!; %U üJ5:i US; %€Γ-4.?Κ;4.13 íífgh-resfiimhm muu speemtmétry {ESí-t/HR, £$i~/LR}:
Bmpirkal lbrmik· C4; iIiSl Ci N{> 04 l'M-rHi' calcidaied; 7i 7.2vS i Hvkiií mmmé< 'ni.2954
Exaia pb 4S4< S^d'S-Chioro-l-lhSiSVSdMóCpitjllÉ^^lmcthvikJ.d-dilhYilyokoqtimniii^ líl/í}'yl|fe«ApiiyisplifenyÍ!''A!-'íS"Cy^nn~l''feíhyh2''m«íhyl"íí/"Pj:rrol~3"yl}"fV"{4·' hydrnsyp!i«?yví|kí2''dinRdhyl4ll-pyrmk>3''Car!>nsiy«Ídfe hydrodtlorkle
The tille compound o ohUuned io nsxonhsnise wiih da: procesa oí’ Uarnpío 1 mang ihc acia oí iBeparMkni ! and ¡he oompound oí 1’repuraUon 3! in Stop Λ, asid lite compourid oí
Pnp&amp;mil&amp;ñ Mn id Stcp O, The. componed obtamed is oonverted tuto «ah íonm and lyophilised ás described in lite pencod popero tu abmin ihc fule contpoimd.
Eiemtfíiíüí mknmmiiy.hh: f%, Íhem'eúcai;me<m¿rerf$ %€US.7!;6$U>; %NU.?8'S.3i; %U!C!,35í!d.fS; ^;ί:ύ,02:4.32
MigM^mMMiém mm^evfrmííei^ β$ΜΜΜι MSSMUKfc Empine»! foratiik:. C\> C1 H, 04 fM+Hf calcntaed: 731310? íMNTIf mea.sured: lil Jií 06 Íi;ai]ilkÍÍI^'-^'C:!doro4~fH^^}^4^H^»rpbídi^4^i}pr8py!|a,4~ iddydr«isiH;|idi^lÍ^>-2(I#Í}~yl]<'sar!)OTij1)phi^yÍ)-^l4“h>!dms>l>Ísemd)>>Ií2^itoelh>>i''í¥-| l^l®m^ydnjíiirs»~3^'l}"I/f-|nrsmd"4~^l|'íliif-pymde»3~e»rtííj!3s»mkliB hydraehfortde The tblo compoond ís' óblame*! a> ae«e«knv.c with the piceas oí 1 xampte I us.m§ the acid of Preparatton 1 and íhe compound oí Prepara? so rs 6! i» Stop A. ami íhe compound oí hepataí m '' n Sup ^ 'Pe se^xsuid o» ?i \y i ter\*i\\í η?«ί a? ϊι^ΐι ano lyophdXod as dexenheel ?n ihe panera! procesa 10 obtmu the tule componed
EjfemeftM:WÍemim&amp;i¡0i$; {% íhi?&amp;?eticahfmMm3ͧ *tíC »vt <*l M 4! o?' 8$ ' \ ^ Μ 10 ' * * A'l 4 4 ' 4 '4 iítgh-re$útfition mas* ^peemnmfty {F>Sí ",ΉΗ, PSh /,60. I niponas ¡onnulo Cu ÍL c ' N< (X (M3íf caiakded: 763.3369 jM *!·!)' measured: 763.3371
Esansplc 4éI>5-d'5-Cl?kro4X|{3^T3-13XMori>toMi^4"yi)prdpyIX3,4~di!fydr«l$^-dimefbyl»/V»pb«isyl-llí«pysTal«>-3^»rl>ea»mid<ilst>dr«>e6!^r|de ! he tale man pe and - a o- mred m eeeejdaacc o sth ti», piocess oi rxampe |. usinp u?e ackt oí Prepuratíon 1 and the eompom! oi Preparador* 6* i» Siep A.; a«d the corupound oí Preparation 2*#" ín Step C\ » being tnalersfood that Step (J ís no? carneo out. ! he compound obhuned m eomeucd mn> salí tbms and !yophiM^|^íÍ^^|d procesa tn etnaiu thc-'Ude coippoimd,
Mternmmi Mzrümafysh; {\ the&amp;retieaíimiwmréfy %C> 67.44:66.6B; %H-6.05:5.HÜ; %M-! OJ730.95? «4;Cf ~d .63;4.57 txaw ^pevtnmiefn· (RSifZBM)?
Effipkkdt fepMlbrC^v ^4S Oi'H; Oí [M+Hf: ealcdaieíl: 729.3214 |Μ·1Ή|: pto&amp;stpf$!: 729-32||
El3^MkÍ^.l.^"OO-:Í^0rjÍ^'Hj3A3''34mffr|:ÜH?ÍI»'4~yjmg|ln'II---3,4'dllwdr^bfHjyl^j>Ikj' 2íI //)-ylIcarfe«»>!Í) |she»yl}”¡V~{4~fayíi!my|ihe»ySH &amp; x m. «I- 3 -y i}-- í H~py r ro te ~3~ο&amp; r h o x a mid o fhe tule compound ts obuñned m aeeordfmi. e wUh the procer <>f l:\amplc \ «sing the aeki of Preparas ten ¡ and iho compoond oí Prepuruhou .'V m Stop Λ, and the nppsoprsate asnino sn Stop ( . Eíementahmcívmiiíysk; {%< iheoteticahmemureé} %í>0?,1 0:66.64; 3411-5.61:5.40: teN-10.30:10.24 Hígh~?esiRmúm mes* vpee/rnmeOT (RS^i/HR, RSl-ZLE);
Emplrkal !%mdía:C^:3tes< Cl M5 C% IMJif caiutlaled: 680,2634 ¡ 841J! \' pipasumd:: 6S6>26 3 '' Ε»Μ«,Α-α -l;:íh«xyp>Tjmyí»'5"ylí»5“{5''f1»ara'-2“||Í^S)'^«<í«srpb»lm“4·' yh»eíhyíí“3>4-ílíhyflroisof|uÍRoií«“2{l//)“yl|carboayl||>heayl)''^»<4“hydr«x|fph«!ii|,l):« i Jtehmdhyi" I If'py rrole* 3“€&amp;rlmaiEKÍ<le
The tifie eosupound te obtained in aceordanee whh the procesa o?' Fxampte ! usiog. the ackl oí Preparasteis 8 and the eotepostósl oí Ptepartetest 3' in Stop Λ, and the enmpound oí Preparasteis 30” in Stop C. Ríemenmi mkrmmufysh: {%? tkeoteReiü;meusun‘d) 34í2te>teí2:é?J2p%PteteJ6;5.6te %N=1 602:11,43 ljearbe**$ I)'\\-C$>líytlr» sypyrkiitt' 2-v!)«! *2»tf ira*Ü*y kY~f I -dmdhy MII- pv rro lo 11,3-é] py ri d I n -5"% lp! //*pvri>deAI<arbíssataildfe dihydroehtaricle 1 v t»^L oo 's\\'!:>v·. s\ oo o \ \ m ,o v vSh o onfh ü v > en p\ totpk. t nsoig too u so oí Ptepandioo I aui fhe componed oí Prepararon ' m Stop A, and tito emttpotnC oí Prepa^ itH'U Ά'! '.o Stop C lito eompnund oHamed o contened tofo vtH ío¡m and lyophtoned as deee.íUv¡j in the general procesa to añudo tho litis compourtU. Hígh-resehtthw mma specJnmumy fáSMift, ESI!VIH): fippiríeal tornada: (,u 11^:0 p? Ct4 |M t-H): calculatod: 7dO<2®3; )\PUV metíate ed: oOASd? 2f I /#hyi|carha»>'i| |»lu'»ylVíV^S - sn amol v2 'dimoíliyklM^|>yrralAkyIkiV~-|'4'' feydrtíaypbattyi}-lU-dÍMstltyk1#/-|>ytay>loSk'«syr|ffaÉiiiplbfif«tetíÍpi'iÍp 1 la.- utie eomposaü is ol-tainco; m accordance with tlva procesa oí Exantpk i ttsing tho sea! o jkepatatíon du o*u tho * on>^Hv nd ot preparador V tn Stop a, and the comportad oí Prepararon !S" sn Stop C Πν coroponnd nhtained ts con ^ otted rnto salí íomt and lyophihsed as describid in tho genera! procesa to obfatn tho tifie compeond, h'kffiiwhtf mknnímiiy$i$t f'A} i^tiXnkeüi'ímemured'i
lVt η- ΊΛ 04 a * A «:t < 6^ 5 Γ o «.N 11 H spo^n 4 o - tN
Bigkmámimkt? mms spectnmtetry (ES¡+);
Propinen! íonmsht: €.. I! . C! N, O. 4? '· 4>; ΐί :>&amp;. |M Hj'eaíenlcded; |M HJf mensurad: ?l?.do>2 2(IH>*yl|<^rl^»3fl)ph«nyi)~/\M4^ll^3^'|tta^Í1í*ljMli^l^^^^^t'^^ pynwl»4yvi}' íH"py r raÍe\Vear boxamtde hydmebk*ritió I he tole coíapoura! ss uhOhaed >n ae* oídat* e v*tíh (he pee>. ^5 <9 * vsmi^° 1 ^s*!se a0i^ of Prepmaliou 29 and Ihe compoSÉiiéi iií PropíÉation 3* m Stop A» aüt4 &amp; eompountl $t Pr^fpaikp F In Stop :€L· The compound fhtpood h spny«!i:gsd íM© ®Út i.» ;Shd lyophíHsed as described m íhe general procesa (o ohiain (he ttüe componed. 67cm&amp;tíai {menamúh'V'*? F<· fhtwreífcakmmsamí} V'¿¿>66.77:62.82; %¿F5.63:5.2F %N-=I 1.74:1 i,75; %€Γ-5.0Ι;5.23
Higfy-mmi&amp;tkm mms tpectrflmefry (ESE FUE ESE i.E); Íimptíieal hnTnúkc, Γ;ϊ ΗμϊΓΐ N., Os [M^fcaieuiated: 679.2794 {Mfhlf measured: 679,2796 £lSSSliÍÍ2íS"<$4‘2d:en>'2'613x4Hlilmlrtdsapi6acdhx3|lli)»yl|e^rÍH>Tí3'l}plie«yl)'-M {4'hydmi>fphenyl)'1J'dlinetfe>l-'A"phen>d*l^pyr:rál^^esrbóxáMÍtle I o ode cerne n n<, m o 'teuocd i,' eiv,> d,eiee ^e\, (he , »eee^ íA -¡ ^arnylo I \ ^ ('<' (he «e c, o! hepanstioa i uid MFU kv^hv* otMtqumo *e *' Skn \ aná ido éümpupíl »# Preperaikm 7.1 m Síep C. Eíemetimi mkroanafysiss (%, thmniic&amp;L'measwsd}
FF "2F?;F ^ Fih * 2F52F, B,6 7.2’* 7M
Hfgh~re$&amp;fatwn mn\* speemmmry (ESí hl'VHR ead US .It'AF i ' r\ el |η?"Η I» s (6 <. \ O iTvl-Hf caloilated: 576,2048 |Μ6|||: ’ ifífmufed: 576:1067
IS&amp;BMSQMáHB£át»SB!IE fhe ihsereseenee polarisaütm tests weie csrried out ou roictopieles (484 vedis). The Bdd proldo, lahelíed (htstag-Bd-2 sueh that Be!··;: eotTesponds to the i.hbñ'otRB^ psknary <ioeev^<m mavsber; RiualM, at ;s fosal eonmsttathm of 1' OdUsá M, >*¡ auxed vistts a OtíOíCNcení pe pude i í hsnteseem RHuAQl RRMAUDI N 4QYt% at a ñ nal eoncemratsoss of kOOxlO'YM neo hollar soloUotvCHepes· 1.0 jsM, NsCl 150 mlVb TweenÜQ 0.05%,-, nH 7 4y |p the prese«ee or ahsenee of ipereadog etmcestratioss of test eotstpoonds. Altor incofeation for2ho«rs, the ffuorescmee polyrisasion O measurod.
The resolta are «Ajnesfwd m ΪΟ5* (íhe conceoóatioo oí eompoomi thal inhibid fluorescente polañsaiion by 50 %} asid are preaemed in Tabie 1 bdoxv. íhe resuiH show That Ose compounds ofthe inx'entioo ínhtbsi interaetion between the Bd-2 proteiti Pisa the llstoreaoeni ptíptídedeseriKa! hereksheioro, tmXMMMMLmsmmám 1 he e> íotoxicñx xUsdses were cerned oUí on the ROM: i 1 foui sesma Tomosrr lose. I'he edis are distribnted oato 'θα. topean <v ·, \putvd e the .ot uOipomids tes -4a he >, » l ile oeti viabilhy is then quantíüed by a colortmeme· assay*the Mierocultee Tetrasoltut» •Vvesy (Cáncer Ros , W87. 47, 0.40*042), 1 ío íesuíts ase evos caí ul n- K v Rhe ee>ü*.co?raUnn ol\ esopound :hat iohO-'t?<. eO x otbdity b> 50 do and ate nre.*etsieu m ! able I 'v4e--v 1 he rosolis show ihd lite eompotínds ofthe investí iots ose eytotodp.
N!> not detemúnod EXAMEtE C; Indoctieo oí eassmss aethdtv in viva,
The abdity oí the compoumfs oí the mvenbon to activare caapa.se 3 ís evahsuted so a xenngrail modo! oí RS4;11 leukaeona cello.
1*10’ RS4;li cello are |pafe4i íídotí 0CÍB siraisd. 25 to 30 days altar the gmlk the anímalo are treaíed orady wlth the. vanouo eompounds. Sixteen honra after treaünem» ihe &amp;h4 the campase 3 acüvity is measurcd la One turnoor lyeaieo. íhss encunado tooasnrene'nt earned ont b\ osayntg the appemanee oí a 1 ha «le eme eleavage produei (DEVDasK activity, Promega). it is expsssed m factor corresponding to the rallo horneen íhe two ρ$ν$ρ||τ:%Τ s resPd mire dh t k e K the neto U> to· the i. otare i once, the results show ihat the eoropcunds oí the Invención are capadle oí ltkÍOétd| apopéis m RS4;I I iomour cells in mm,
The ahtlhy oí the eompoumls of the imeutlon to actívalo casposo 3 lo evalaated in a xenograít modo] oí RS4;1 l knkaenda celia, jxdf RS4J1 ioih ate getfted snb~eonmwnsh mío lonntmosupp ressed falce (Sí 113 stnñn). 25 to 30 days aíter ihe grait. the animáis are ireated orally wlth the various comptamds. Aíter (rcamtenk the íumour msosoo are reeovered and lyoed. and the eleaved (activated) tono oíeaspase 3 o goandñed in the tumonr lysates.
HiO puamitkadon is cerned toa usina. ihe !SMeso Scalc Discovcry (SISO) HURA pnukonr test. xshieh spfeíikxdh as-sos ihe eleaxed hnm oí caspas* * \t ,s expresad to ihe form oían::aebx?pldniae&amp; cMpsi; % In tito troaloct mico divkied bv the qoantky oí dcaved caspase 3 ín the control mico.
The redolis show llmí lítb eompbubhs oí ihó mwbtlbb dio oapabk bi lEditblbg appMis :m RT4;f I O.oooor cdk ó? vmh teifáisstj&amp;vsr^ja^^
The aeüvífy oí (he eonspouoda oí (he uryemsoo % pTuaied os ,5 ΌοίοροίΟ modd OÍ R84; s i IcoLaemos ceiis, KUi RS4;U ce»h aje gsaOed sub-eutancou*^ uno onoomosoppa^ed 0000 tSOO áhíain). 25 to 30 daos a ñor itie grah, whm the lumoor mass has reaehed abouí ! 50 mm\ (he rnke are Srsáteá. orally vvith the varíous eompoundx so two dfííerent regimos (OaOv (rexuraesit íor flve days per week Ior ovo wcoks. or two -treaunenís \veekiy ior iwo weeks}. The roososir masa is sneasured iwice weekly iroso (he starí ot (reatsoent,
The residís obíamed accordsngh shtn\ ihaí Éi eobrpuida oí liio sovaatsosi as'o e.apab(e oí iadsiteg srgollüaoí mmbbí sepse^km. peridd. &amp;l£&amp;li$FlkE FtRharmaeeP^^ .§>»fe¡ji£y*^W>x>>>>>>s>S>S>>>>>^ ' llOíífahlda uadaisOng a José oí5 sssg oía ( M>fl? 5 g.
Wht-ai siareh................S
Mx-.s/.e starch........................................<..........-......................-<>.*:>?>o···*<«- -·w 4$ S
i. ac(080 ..........,y.? .... 1.:.,:.y. -. :.y .:.„,y:.::.... j .:000 · O .......>S -· '*0·: '5^ R
'-•S
Magne.skssrs s( ea r p! o...............................................,..---........................................... * g
SHiea......................................................................................................................···· I S
Byd roa yprppy Ice huloso ...... .,.,..:,:¾.. - d

Claims (5)

  1. Ssahadáteú igénypAktók
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  2. 3. Ak k vagy 2, sgxhryposa »x«dnthl; képleía veg>ok't, ahoi mimlA(i mmd A*je!ent<&amp;e metiícsopon. ·4χ A» k-3. igénxpoidok harmcRiU kx'pleTO vegyUbi# ahol.T jelíaatéa®··mdil% «¡ru.ísomtttíi-, N (‘kjmAdpíjv^O'i-Hwaftsl-, 2^m^r^hn-A^íkwl>, p-(wfrfy3m' i jI«ck»vn<'vt«K hklroNsvask, |x>-(diiiHSd.^msvk'V'cO«5Ciíkx Ivx Aíkímpiráí ísks^ 2,. S <.]!; xPloxadü- S{ i //>-ikvví-; l -i>'<a-6· a,\sp od '|lyptp I x xul 11 <k»k> r O* x^pd ν.χ?χ inV <<uu x¡\so|V5'5:.
    3. Ax 3. sjpdiypoíií S2#ri«ti <0 képlekt vegyídet ;
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  4. 33, A JI, IgÉíypiísS
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