HRP20230865T1 - Kompozicije egzosoma i postupci za njihovu pripremu i primjenu za reguliranje i kondicioniranje kože i kose - Google Patents
Kompozicije egzosoma i postupci za njihovu pripremu i primjenu za reguliranje i kondicioniranje kože i kose Download PDFInfo
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- HRP20230865T1 HRP20230865T1 HRP20230865TT HRP20230865T HRP20230865T1 HR P20230865 T1 HRP20230865 T1 HR P20230865T1 HR P20230865T T HRP20230865T T HR P20230865TT HR P20230865 T HRP20230865 T HR P20230865T HR P20230865 T1 HRP20230865 T1 HR P20230865T1
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- skin
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- heat shock
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- 210000001808 exosome Anatomy 0.000 title claims 14
- 238000000034 method Methods 0.000 title claims 9
- 230000001105 regulatory effect Effects 0.000 title claims 4
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- 230000035939 shock Effects 0.000 claims 13
- 239000002537 cosmetic Substances 0.000 claims 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 5
- 230000002500 effect on skin Effects 0.000 claims 3
- 230000003938 response to stress Effects 0.000 claims 3
- 239000004017 serum-free culture medium Substances 0.000 claims 3
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
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- 210000000130 stem cell Anatomy 0.000 claims 2
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 1
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- 108010022452 Collagen Type I Proteins 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 claims 1
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- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 claims 1
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 claims 1
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- MSCCTZZBYHQMQJ-AZAGJHQNSA-N Tocopheryl nicotinate Chemical compound C([C@@](OC1=C(C)C=2C)(C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)CC1=C(C)C=2OC(=O)C1=CC=CN=C1 MSCCTZZBYHQMQJ-AZAGJHQNSA-N 0.000 claims 1
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- 239000003963 antioxidant agent Substances 0.000 claims 1
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- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 claims 1
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- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 claims 1
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- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims 1
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- 229950009883 tocopheryl nicotinate Drugs 0.000 claims 1
- 230000036572 transepidermal water loss Effects 0.000 claims 1
- 239000011708 vitamin B3 Substances 0.000 claims 1
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- -1 vitamin B3 compound Chemical class 0.000 claims 1
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- QUEDXNHFTDJVIY-UHFFFAOYSA-N γ-tocopherol Chemical class OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-UHFFFAOYSA-N 0.000 claims 1
Classifications
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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Claims (15)
1. Topikalna kompozicija za reguliranje kozmetičkog stanja kože ili kose, kompozicija koja sadrži efikasnu količinu izoliranih egzosoma koji imaju povišene nivoe molekula odgovora na stres toplinskog šoka, pri čemu su izolirani egzosomi egzosomi toplinskog šoka izvedeni iz mezenhimalnih matičnih stanica podvrgnutih toplinskom šoku placentalnog ili adipoznog porijekla, i nosač i pri čemu su egzosomi toplinskog šoka proizvedeni postupkom koji obuhvaća:
(a) kultiviranje matičnih stanica u mediju za kulturu, pri čemu kultivacija uključuje korak toplinskog šokiranja matičnih stanica u mediju za kulturu bez seruma povećanjem temperaturu kulture na 41°C do 43 °C tokom 1 sata do 3 sata, i pri čemu medij za kulturu bez seruma sadrži egzosome koji imaju povišene nivoe molekula odgovora na stres toplinskog šoka; i
(b) izoliranje egzosoma koji imaju povišene nivoe molekula odgovora na stres toplinskog šoka iz medija bez seruma.
2. Kompozicija prema patentnom zahtjevu 1, pri čemu egzosomi toplinskog šoka imaju povišene nivoe HSP70 u odnosu na β-aktin u usporedbi sa usporedivim egzosomima izvedenim iz mezenhimalnih matičnih stanica placentalnog ili adipoznog porijekla kultiviranih bez toplinskog šoka.
3. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-2, koja dalje sadrži od 0.1 do 20% sredstva za vlaženje, gdje po izboru sredstvo za vlaženje sadrži jedno ili više od pantenola, derivata pantotenske kiseline, glicerina, glicerola, dimetikona, vazelina, hijaluronske kiseline, ili ceremida, i njihovih smjesa.
4. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-3, koja dalje sadrži spoj vitamina B3.
5. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-3, koja dalje sadrži tokoferol nikotinat.
6. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-3, koja dalje sadrži anti-oksidans, opcionalno jedan ili kombinaciju tokoferola ili estara tokoferola.
7. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-6, pri čemu egzosomi toplinskog šoka su osušeni zamrzavanjem.
8. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-7, pri čemu:
(a) medij za kulturu bez seruma je bez životinjskih proizvoda; i/ili
(b) kompozicija u obliku tekućine, losiona, kreme, gela, pjene, gušće pjene (mousse), spreja, paste, praha, ili čvrste supstance.
9. Kompozicija iz bilo kojeg od patentnih zahtjeva 1-8, pri čemu je kozmetičko stanje kože ili kose izazvano UVB zračenjem.
10. Postupak izrade topikalne kompozicije za reguliranje kozmetičkog stanja kože ili kose, pri čemu postupak obuhvaća kombiniranje efikasne količine egzosoma toplinskog šoka prema bilo kojem od patentnih zahtjeva 1-9 sa nosačem.
11. Postupak za reguliranje kozmetičkog stanja kože koji obuhvaća topikalno administriranje kompozicije prema bilo kojem od patentnih zahtjeva 1-9, pri čemu kozmetičko stanje kože obuhvaća integritet kože, sadržaj vode ili vlažnost kože, transepidermalni gubitak vode, perutanje kože, i ljuštenje, debljinu kože, zatezna svojstva kože, pojavu dermalnih finih linija i bora, teksturu kože, veličinu pora kože, glatkoću kože, staračke pjege na koži, ten kože, ili pojavu ožiljaka i abrazija kože.
12. Postupak prema patentnom zahtjevu 11, pri čemu egzosomi toplinskog šoka uključuju proizvodnju kolagena I dermalnih fibroblasta.
13. Postupak prema patentnom zahtjevu 11, pri čemu egzosomi toplinskog šoka induciraju proliferaciju dermalnih fibroblasta.
14. Postupak prema patentnom zahtjevu 11, pri čemu egzosomi toplinskog šoka inhibiraju eksprimiranje inflamatornih citokina.
15. Postupak iz bilo kojeg od patentnih zahtjeva 10-14, pri čemu kozmetičko stanje kože ili kose je uzrokovano UVB zračenjem.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562199696P | 2015-07-31 | 2015-07-31 | |
| EP16833569.3A EP3328397B1 (en) | 2015-07-31 | 2016-07-28 | Exosome compositions and methods for preparation and use thereof for regulating and conditioning skin and hair |
| PCT/US2016/044458 WO2017023690A1 (en) | 2015-07-31 | 2016-07-28 | Exosome compositions and methods for preparation and use thereof for regulating and conditioning skin and hair |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20230865T1 true HRP20230865T1 (hr) | 2023-11-10 |
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| HRP20230865TT HRP20230865T1 (hr) | 2015-07-31 | 2016-07-28 | Kompozicije egzosoma i postupci za njihovu pripremu i primjenu za reguliranje i kondicioniranje kože i kose |
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| US (2) | US20180147420A1 (hr) |
| EP (2) | EP3328397B1 (hr) |
| JP (2) | JP6980955B2 (hr) |
| CA (2) | CA2993227C (hr) |
| ES (1) | ES2952050T3 (hr) |
| HR (1) | HRP20230865T1 (hr) |
| HU (1) | HUE063486T2 (hr) |
| PL (1) | PL3328397T3 (hr) |
| RS (1) | RS64469B1 (hr) |
| WO (2) | WO2017023690A1 (hr) |
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| US11596656B2 (en) * | 2016-08-23 | 2023-03-07 | Albert Einstein College Of Medicine | Stem cell-produced microvesicles for treating tendon pathologies |
| CN107080753A (zh) * | 2017-04-20 | 2017-08-22 | 深圳市赛欧细胞生物科技有限公司 | 一种人脐带间充质干细胞源外泌体的美容制剂 |
| CN107006452A (zh) * | 2017-04-20 | 2017-08-04 | 深圳市赛欧细胞生物科技有限公司 | 一种人脐带间充质干细胞源外泌体冻干保存方法及其应用 |
| CN107245472B (zh) * | 2017-06-08 | 2021-08-06 | 北京智能宝生物科技有限公司 | 一种人间充质干细胞外泌体冻干粉的制备方法、使用方法 |
| WO2019004738A2 (ko) * | 2017-06-30 | 2019-01-03 | 주식회사 엑소코바이오 | 지방줄기세포 유래의 엑소좀을 유효성분으로 포함하는 조성물의 피부염 개선 용도 |
| JP7037205B2 (ja) * | 2017-06-30 | 2022-03-16 | エクソコバイオ インコーポレイテッド | 幹細胞由来のエキソソームを有効成分として含む組成物のかゆみの抑制又は改善の用途 |
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| WO2019103381A1 (ko) * | 2017-11-24 | 2019-05-31 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 조성물의 피부장벽 강화 내지 기능 개선 용도 |
| JP2021506801A (ja) * | 2017-12-14 | 2021-02-22 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | 精製されたエクソソーム生成物、作成方法及び使用方法 |
| WO2019139762A1 (en) * | 2018-01-09 | 2019-07-18 | Zen-Bio, Inc. | Exosome compositions and use thereof for joint disorders and diseases |
| US20210244769A1 (en) * | 2018-05-04 | 2021-08-12 | Figene, Llc | Pain-reducing effects of fibroblasts and treatment of pain |
| RU2710368C2 (ru) * | 2018-05-29 | 2019-12-26 | Станислав Евгеньевич Волчков | Способ получения и концентрирования микроРНК-содержащих экзосом мультипотентных мезенхимально-стромальных клеток для применения в косметических и лекарственных средствах для стимуляции регенеративных процессов и замедления процессов старения |
| WO2019231133A1 (ko) * | 2018-05-31 | 2019-12-05 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 모공 축소용 조성물 |
| KR102649069B1 (ko) * | 2018-05-31 | 2024-03-19 | 주식회사 엑소코바이오 | 줄기세포 유래의 엑소좀을 유효성분으로 포함하는 안면 홍조 개선용 조성물 |
| CN108715834B (zh) * | 2018-06-01 | 2021-09-14 | 天晴干细胞股份有限公司 | 一种富含cd41+、cd81+微囊的血小板裂解液制备方法 |
| KR102176845B1 (ko) * | 2018-10-02 | 2020-11-10 | 주식회사 스템온 | 유도된 엑소좀을 포함하는 피부 재생 및 상처 치유용 조성물 |
| WO2020180311A1 (en) * | 2019-03-06 | 2020-09-10 | Zen-Bio, Inc. | Plant-based exosome compositions and use thereof for rejuvenating skin |
| BR112021021676A2 (pt) * | 2019-04-29 | 2021-12-21 | Direct Biologics Llc | Uso de preparação de exossomo de células-tronco mesenquimais para a preparação de composição para o tratamento da doença periodontal |
| JP2021040551A (ja) * | 2019-09-11 | 2021-03-18 | 富士フイルム株式会社 | 培地組成物、キット、間葉系幹細胞組成物の製造方法、間葉系幹細胞組成物、間葉系幹細胞、細胞培養上清および細胞外小胞 |
| CN110559254A (zh) * | 2019-10-20 | 2019-12-13 | 张新梅 | 一种外泌体组合物、其制备方法及应用 |
| CN110721198A (zh) * | 2019-11-08 | 2020-01-24 | 赵凯 | 一种预防癫痫产生脑损伤的外泌体喷雾的制作工艺 |
| WO2022158816A1 (ko) * | 2021-01-20 | 2022-07-28 | 성균관대학교산학협력단 | 줄기세포 유래 엑소좀, 히알루론산, 및 bdde를 포함하는 피부 주름개선용 필러 조성물 및 이의 제조 방법 |
| KR102368442B1 (ko) * | 2021-02-19 | 2022-02-28 | 주식회사 엑소코바이오 | 생리활성이 강화된 엑소좀의 생산방법 및 이의 응용 |
| CN113041259B (zh) * | 2021-03-23 | 2023-05-12 | 哈尔滨科技实业开发有限公司 | 牙髓干细胞外泌体制剂及制备方法和应用 |
| CN113384518A (zh) * | 2021-07-27 | 2021-09-14 | 山西遗传资源细胞库有限公司 | 一种干细胞外泌体应用于面膜的方法 |
| WO2023079655A1 (ja) * | 2021-11-04 | 2023-05-11 | 株式会社リバティソリューション | エクソソーム抽出方法 |
| CN113940912B (zh) * | 2021-12-09 | 2024-04-16 | 深圳市泓浩生物科技有限公司 | 抗衰老的组合物及其应用 |
| CN116099056A (zh) * | 2023-04-07 | 2023-05-12 | 云南云科特色植物提取实验室有限公司 | 一种含仿生纹理结构的人工皮肤及其制备方法 |
| CN116790482A (zh) * | 2023-06-12 | 2023-09-22 | 成都诺医德医学检验实验室有限公司 | 一种刺激皮肤类器官分泌外泌体的方法 |
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| US5348945A (en) * | 1990-04-06 | 1994-09-20 | Wake Forest University | Method of treatment with hsp70 |
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| EP1514539A3 (en) * | 2003-05-14 | 2005-05-04 | Signal Investment & Management Co. | Micro-encapsulated topical analgesic for pain relief and sleeve comprising it |
| US20060143782A1 (en) * | 2004-10-07 | 2006-07-06 | Sherry Ajluni | Glove with skin lotion |
| WO2008086358A1 (en) * | 2007-01-08 | 2008-07-17 | University Of Southern California Usc Stevens | Skin wound healing compositions and methods of use thereof |
| JP5718648B2 (ja) * | 2008-02-22 | 2015-05-13 | エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ（エイ・スター） | 間葉系幹細胞粒子 |
| KR101480362B1 (ko) * | 2010-08-23 | 2015-01-28 | 주식회사 강스템바이오텍 | Ｎｏｄ２의 아고니스트를 처리한 줄기세포 또는 그 배양물을 포함하는 면역질환 또는 염증질환의 예방 또는 치료용 약학조성물 |
| SG189438A1 (en) * | 2010-10-18 | 2013-05-31 | Agency Science Tech & Res | Use of exosomes to promote or enhance hair growth |
| US9119974B2 (en) * | 2011-03-04 | 2015-09-01 | Ahmed H. Al-Qahtani | Skin cream |
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| JP3171007U (ja) * | 2011-06-28 | 2011-10-13 | ヘルスアシスト株式会社 | 腕肩美容パックシート |
| US9427450B2 (en) * | 2012-01-31 | 2016-08-30 | Xon Cells, Inc. | Therapeutic immune modulation by stem cell secreted exosomes |
| EP2687219A1 (en) * | 2012-07-18 | 2014-01-22 | Universität Duisburg-Essen | Use of preparations comprising exosomes derived from mesenchymal stem cells (MSCs) in the prevention and therapy of inflammatory conditions |
| PL2972193T3 (pl) * | 2013-03-13 | 2020-08-24 | University Of Miami | Sposób izolacji i oczyszczania mikropącherzyków z supernatantów hodowli komórkowej i płynów biologicznych |
| US20160120805A1 (en) * | 2013-06-05 | 2016-05-05 | The Trustees Of Columbia University In The City Of New York | Exosomes for orofacial diagnostics and therapeutics |
| JP2015101541A (ja) * | 2013-11-21 | 2015-06-04 | ポーラ化成工業株式会社 | 皮膚関連酵素活性化剤 |
| JP6253361B2 (ja) * | 2013-11-21 | 2017-12-27 | ポーラ化成工業株式会社 | 皮膚外用剤 |
| US9464272B2 (en) * | 2013-12-12 | 2016-10-11 | Stemnion, Inc. | Cell-derived composition |
| CN104488850B (zh) * | 2014-11-28 | 2016-11-02 | 广州赛莱拉干细胞科技股份有限公司 | 一种制备人羊膜间充质干细胞外泌体冻干粉的方法 |
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2016
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- 2016-07-28 EP EP16833568.5A patent/EP3328403A4/en not_active Withdrawn
- 2016-07-28 CA CA2993227A patent/CA2993227C/en active Active
- 2016-07-28 HR HRP20230865TT patent/HRP20230865T1/hr unknown
- 2016-07-28 WO PCT/US2016/044458 patent/WO2017023690A1/en active Application Filing
- 2016-07-28 RS RS20230599A patent/RS64469B1/sr unknown
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2018
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| EP3328403A1 (en) | 2018-06-06 |
| CA2993227A1 (en) | 2017-02-09 |
| EP3328397B1 (en) | 2023-07-12 |
| EP3328397C0 (en) | 2023-07-12 |
| PL3328397T3 (pl) | 2023-10-16 |
| JP2018522593A (ja) | 2018-08-16 |
| US20180147420A1 (en) | 2018-05-31 |
| CA2993227C (en) | 2020-07-21 |
| RS64469B1 (sr) | 2023-09-29 |
| US20180177828A1 (en) | 2018-06-28 |
| CA2993224A1 (en) | 2017-02-09 |
| JP6719559B2 (ja) | 2020-07-08 |
| EP3328397A1 (en) | 2018-06-06 |
| EP3328403A4 (en) | 2019-04-17 |
| HUE063486T2 (hu) | 2024-01-28 |
| EP3328397A4 (en) | 2019-04-03 |
| WO2017023690A1 (en) | 2017-02-09 |
| WO2017023689A1 (en) | 2017-02-09 |
| ES2952050T3 (es) | 2023-10-26 |
| JP2018522071A (ja) | 2018-08-09 |
| JP6980955B2 (ja) | 2021-12-15 |
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