ES8504930A1 - Procedimiento para preparar un plasmido de produccion - Google Patents

Procedimiento para preparar un plasmido de produccion

Info

Publication number
ES8504930A1
ES8504930A1 ES533214A ES533214A ES8504930A1 ES 8504930 A1 ES8504930 A1 ES 8504930A1 ES 533214 A ES533214 A ES 533214A ES 533214 A ES533214 A ES 533214A ES 8504930 A1 ES8504930 A1 ES 8504930A1
Authority
ES
Spain
Prior art keywords
sequences
prokaryotes
preparation
gene products
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES533214A
Other languages
English (en)
Other versions
ES533214A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of ES8504930A1 publication Critical patent/ES8504930A1/es
Publication of ES533214A0 publication Critical patent/ES533214A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA PREPARAR UN PLASMIDO DE PRODUCCION.COMPRENDE: A) INSERTAR EN UN PLASMIDO DE EXPRESION, UN GEN ESTRUCTURAL, QUE CODIFICA IFN-BABA Y VA A CONTINUACION DE LA SECUENCIA DEL ENLAZADOR; Y B) HACER REACCIONAR AL PLASMIDO DE LA ETAPA (A) CON LIGASA PARA INSERTAR UN PAR-LUGAR Y OBTENER UN PLASMIDO DE PRODUCCION QUE CONTIENE UNA O VARIAS SECUENCIAS DE ADN DE FORMULA (I) Y UN PAR-LUGAR (LUGAR DE PARIDAD) Y SUS MUTANTES OBTENIBLES.
ES533214A 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de produccion Granted ES533214A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19823247922 DE3247922A1 (de) 1982-12-24 1982-12-24 Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten

Publications (2)

Publication Number Publication Date
ES8504930A1 true ES8504930A1 (es) 1985-04-16
ES533214A0 ES533214A0 (es) 1985-04-16

Family

ID=6181687

Family Applications (4)

Application Number Title Priority Date Filing Date
ES528350A Granted ES528350A0 (es) 1982-12-24 1983-12-23 Procedimiento para la preparacion de interferon humano.
ES533213A Granted ES533213A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de expresion per 103
ES533214A Granted ES533214A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de produccion
ES533197A Granted ES533197A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ES528350A Granted ES528350A0 (es) 1982-12-24 1983-12-23 Procedimiento para la preparacion de interferon humano.
ES533213A Granted ES533213A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido de expresion per 103

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES533197A Granted ES533197A0 (es) 1982-12-24 1984-06-07 Procedimiento para preparar un plasmido

Country Status (19)

Country Link
EP (1) EP0115613B1 (es)
JP (2) JPH088868B2 (es)
KR (1) KR930005455B1 (es)
AT (1) ATE62018T1 (es)
AU (1) AU579991B2 (es)
DD (1) DD216043A5 (es)
DE (2) DE3247922A1 (es)
DK (1) DK576983A (es)
ES (4) ES528350A0 (es)
FI (1) FI86991C (es)
GR (1) GR78766B (es)
HU (1) HU202277B (es)
IL (1) IL70523A (es)
MX (1) MX9202878A (es)
NO (1) NO173742C (es)
NZ (1) NZ206700A (es)
SU (2) SU1366064A3 (es)
UA (1) UA8035A1 (es)
ZA (1) ZA839519B (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3584198D1 (de) * 1984-08-01 1991-10-31 Boehringer Ingelheim Int Neue genetische sequenzen, die durch sie codierten interferon-peptide vom typ i und diese sie produzierende organismen.
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
IL95223A (en) * 1984-12-06 1992-08-18 Synergen Biolog Inc Dna sequence for production of serine protease inhibitors
FI90990C (fi) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Rekombinantti-DNA-molekyyli, transformoitu isäntäorganismi ja menetelmä interferonin valmistamiseksi
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
DK151585D0 (da) * 1985-04-03 1985-04-03 Nordisk Gentofte Dna-sekvens
DE3514113A1 (de) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
DE3607835A1 (de) * 1986-03-10 1987-09-24 Boehringer Ingelheim Int Hybridinterferone, deren verwendung als arzneimittel und als zwischenprodukte zur herstellung von antikoerpern und deren verwendung sowie verfahren zu ihrer herstellung
DE3642096A1 (de) * 1986-12-10 1988-06-16 Boehringer Ingelheim Int Pferde-(gamma)-interferon
GB8813032D0 (en) * 1988-06-02 1988-07-06 Boehringer Ingelheim Int Antiviral pharmaceutical composition
WO1990005186A1 (en) * 1988-11-04 1990-05-17 The Upjohn Company Somatotropin expression using a serratia promoter
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
US5531880A (en) * 1994-09-13 1996-07-02 Microelectronics And Computer Technology Corporation Method for producing thin, uniform powder phosphor for display screens
FR2724665B1 (fr) * 1994-09-16 1996-12-20 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes, plasmides et cellules modifiees

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
ATE25985T1 (de) * 1980-06-12 1987-04-15 Japan Found Cancer Plasmid.
ZA814375B (en) * 1980-07-01 1982-07-28 Hoffmann La Roche Interferons and process for their preparation
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0062971B1 (en) * 1981-03-27 1990-03-07 Imperial Chemical Industries Plc Genetically modified microorganisms
DE3220116A1 (de) * 1982-05-28 1983-12-01 Dr. Karl Thomae Gmbh, 7950 Biberach Mikrobiologisch hergestellte (alpha)- und ss-interferone, dna-sequenzen, die fuer diese interferone codieren, mikroorganismen, die diese genetische information enthalten, und verfahren zu ihrer herstellung
DE3247923A1 (de) * 1982-12-24 1984-06-28 Dr. Karl Thomae Gmbh, 7950 Biberach Neue oligonucleotide und verfahren zu ihrer herstellung

Also Published As

Publication number Publication date
FI86991C (fi) 1992-11-10
ES8503372A1 (es) 1985-02-16
IL70523A (en) 1991-08-16
NZ206700A (en) 1987-11-27
AU2283283A (en) 1984-06-28
NO173742B (no) 1993-10-18
ES8501798A1 (es) 1984-12-01
DK576983D0 (da) 1983-12-14
EP0115613A2 (de) 1984-08-15
DK576983A (da) 1984-06-25
SU1417800A3 (ru) 1988-08-15
FI86991B (fi) 1992-07-31
FI834700A (fi) 1984-06-25
DE3382233D1 (de) 1991-05-02
JPS59162888A (ja) 1984-09-13
ES8506090A1 (es) 1985-06-16
KR840007105A (ko) 1984-12-05
MX9202878A (es) 1992-06-30
ES533213A0 (es) 1985-06-16
JPH088868B2 (ja) 1996-01-31
ZA839519B (en) 1985-08-28
NO834792L (no) 1984-06-25
DD216043A5 (de) 1984-11-28
GR78766B (es) 1984-10-02
FI834700A0 (fi) 1983-12-21
IL70523A0 (en) 1984-03-30
UA8035A1 (uk) 1995-12-26
ES528350A0 (es) 1984-12-01
AU579991B2 (en) 1988-12-22
ATE62018T1 (de) 1991-04-15
DE3247922A1 (de) 1984-06-28
SU1366064A3 (ru) 1988-01-07
ES533197A0 (es) 1985-02-16
HU202277B (en) 1991-02-28
EP0115613B1 (de) 1991-03-27
EP0115613A3 (en) 1986-07-23
ES533214A0 (es) 1985-04-16
JP2637328B2 (ja) 1997-08-06
JPH05214000A (ja) 1993-08-24
KR930005455B1 (ko) 1993-06-22
NO173742C (no) 1994-01-26

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000201