IL70523A - Dna sequence for expression in microorganisms,having a promoter,a ribosome binding site,a linker sequence,a structural gene encoding a polypeptide,plasmids containing the dna sequence,processes for preparing the plasmids and polypeptides encoded thereby - Google Patents

Dna sequence for expression in microorganisms,having a promoter,a ribosome binding site,a linker sequence,a structural gene encoding a polypeptide,plasmids containing the dna sequence,processes for preparing the plasmids and polypeptides encoded thereby

Info

Publication number
IL70523A
IL70523A IL70523A IL7052383A IL70523A IL 70523 A IL70523 A IL 70523A IL 70523 A IL70523 A IL 70523A IL 7052383 A IL7052383 A IL 7052383A IL 70523 A IL70523 A IL 70523A
Authority
IL
Israel
Prior art keywords
dna sequence
plasmids
sequence
expression
promoter
Prior art date
Application number
IL70523A
Other versions
IL70523A0 (en
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of IL70523A0 publication Critical patent/IL70523A0/en
Publication of IL70523A publication Critical patent/IL70523A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to the combination of the promoter/operator region of the tryptophan operon of Serratia marcescens with a synthetic ribosome binding sequence, an expression plasmid which contains this sequence and in which the above sequence is followed by the only Hind III cleavage site in the plasmid, into which site the gene to be expressed can be inserted, and to production plasmids for the expression of eukaryotic gene products in prokaryotes, especially for the preparation of leucocyte interferon.
IL70523A 1982-12-24 1983-12-22 Dna sequence for expression in microorganisms,having a promoter,a ribosome binding site,a linker sequence,a structural gene encoding a polypeptide,plasmids containing the dna sequence,processes for preparing the plasmids and polypeptides encoded thereby IL70523A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19823247922 DE3247922A1 (en) 1982-12-24 1982-12-24 DNA SEQUENCES, THEIR PRODUCTION, PLASMIDES CONTAINING THESE SEQUENCES AND THE USE THEREOF FOR THE SYNTHESIS OF EUKARYOTIC GENE PRODUCTS IN PROKARYOTS

Publications (2)

Publication Number Publication Date
IL70523A0 IL70523A0 (en) 1984-03-30
IL70523A true IL70523A (en) 1991-08-16

Family

ID=6181687

Family Applications (1)

Application Number Title Priority Date Filing Date
IL70523A IL70523A (en) 1982-12-24 1983-12-22 Dna sequence for expression in microorganisms,having a promoter,a ribosome binding site,a linker sequence,a structural gene encoding a polypeptide,plasmids containing the dna sequence,processes for preparing the plasmids and polypeptides encoded thereby

Country Status (19)

Country Link
EP (1) EP0115613B1 (en)
JP (2) JPH088868B2 (en)
KR (1) KR930005455B1 (en)
AT (1) ATE62018T1 (en)
AU (1) AU579991B2 (en)
DD (1) DD216043A5 (en)
DE (2) DE3247922A1 (en)
DK (1) DK576983A (en)
ES (4) ES528350A0 (en)
FI (1) FI86991C (en)
GR (1) GR78766B (en)
HU (1) HU202277B (en)
IL (1) IL70523A (en)
MX (1) MX9202878A (en)
NO (1) NO173742C (en)
NZ (1) NZ206700A (en)
SU (2) SU1366064A3 (en)
UA (1) UA8035A1 (en)
ZA (1) ZA839519B (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3584198D1 (en) * 1984-08-01 1991-10-31 Boehringer Ingelheim Int NEW GENETIC SEQUENCES CODED BY TYPE I INTERFERON PEPTIDES AND THESE PRODUCING ORGANISMS.
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
IL95223A (en) * 1984-12-06 1992-08-18 Synergen Biolog Inc Dna sequence for production of serine protease inhibitors
FI90990C (en) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Recombinant DNA molecule, transformed host organism, and method for producing interferon
DK156072C (en) * 1985-04-03 1989-11-06 Nordisk Gentofte SYNTHETIC DNA SEQUENCE CONTAINING A RIBO BINDING SITE
DK151585D0 (en) * 1985-04-03 1985-04-03 Nordisk Gentofte DNA sequence
DE3514113A1 (en) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt CHANGE OF THE DNA SEQUENCE BETWEEN SHINE-DALGARNO SEQUENCE AND START CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
DE3607835A1 (en) * 1986-03-10 1987-09-24 Boehringer Ingelheim Int HYBRID INTERFERONS, THEIR USE AS MEDICINAL PRODUCTS AND AS INTERMEDIATE PRODUCTS FOR THE PRODUCTION OF ANTIBODIES AND THE USE THEREOF AND METHOD FOR THEIR PRODUCTION
DE3642096A1 (en) * 1986-12-10 1988-06-16 Boehringer Ingelheim Int HORSE (GAMMA) INTERFERON
GB8813032D0 (en) * 1988-06-02 1988-07-06 Boehringer Ingelheim Int Antiviral pharmaceutical composition
WO1990005186A1 (en) * 1988-11-04 1990-05-17 The Upjohn Company Somatotropin expression using a serratia promoter
EP0626448A3 (en) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Process for preparing and purifying alpha-interferon
US5531880A (en) * 1994-09-13 1996-07-02 Microelectronics And Computer Technology Corporation Method for producing thin, uniform powder phosphor for display screens
FR2724665B1 (en) * 1994-09-16 1996-12-20 Rhone Poulenc Rorer Sa PROCESS FOR PRODUCING RECOMBINANT PROTEINS, PLASMIDS AND MODIFIED CELLS

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
ATE25985T1 (en) * 1980-06-12 1987-04-15 Japan Found Cancer PLASMID.
ZA814375B (en) * 1980-07-01 1982-07-28 Hoffmann La Roche Interferons and process for their preparation
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0062971B1 (en) * 1981-03-27 1990-03-07 Imperial Chemical Industries Plc Genetically modified microorganisms
DE3220116A1 (en) * 1982-05-28 1983-12-01 Dr. Karl Thomae Gmbh, 7950 Biberach MICROBIOLOGICALLY MANUFACTURED (ALPHA) AND SS INTERFERONES, DNA SEQUENCES CODING FOR THESE INTERFERONES, MICROORGANISMS CONTAINING THIS GENETIC INFORMATION, AND METHOD FOR THE PRODUCTION THEREOF
DE3247923A1 (en) * 1982-12-24 1984-06-28 Dr. Karl Thomae Gmbh, 7950 Biberach Oligonucleotides and process for their preparation

Also Published As

Publication number Publication date
FI86991C (en) 1992-11-10
ES8503372A1 (en) 1985-02-16
NZ206700A (en) 1987-11-27
AU2283283A (en) 1984-06-28
NO173742B (en) 1993-10-18
ES8501798A1 (en) 1984-12-01
DK576983D0 (en) 1983-12-14
EP0115613A2 (en) 1984-08-15
DK576983A (en) 1984-06-25
SU1417800A3 (en) 1988-08-15
FI86991B (en) 1992-07-31
FI834700A (en) 1984-06-25
DE3382233D1 (en) 1991-05-02
JPS59162888A (en) 1984-09-13
ES8506090A1 (en) 1985-06-16
KR840007105A (en) 1984-12-05
MX9202878A (en) 1992-06-30
ES533213A0 (en) 1985-06-16
JPH088868B2 (en) 1996-01-31
ZA839519B (en) 1985-08-28
NO834792L (en) 1984-06-25
DD216043A5 (en) 1984-11-28
GR78766B (en) 1984-10-02
FI834700A0 (en) 1983-12-21
IL70523A0 (en) 1984-03-30
UA8035A1 (en) 1995-12-26
ES8504930A1 (en) 1985-04-16
ES528350A0 (en) 1984-12-01
AU579991B2 (en) 1988-12-22
ATE62018T1 (en) 1991-04-15
DE3247922A1 (en) 1984-06-28
SU1366064A3 (en) 1988-01-07
ES533197A0 (en) 1985-02-16
HU202277B (en) 1991-02-28
EP0115613B1 (en) 1991-03-27
EP0115613A3 (en) 1986-07-23
ES533214A0 (en) 1985-04-16
JP2637328B2 (en) 1997-08-06
JPH05214000A (en) 1993-08-24
KR930005455B1 (en) 1993-06-22
NO173742C (en) 1994-01-26

Similar Documents

Publication Publication Date Title
IL70523A (en) Dna sequence for expression in microorganisms,having a promoter,a ribosome binding site,a linker sequence,a structural gene encoding a polypeptide,plasmids containing the dna sequence,processes for preparing the plasmids and polypeptides encoded thereby
ES8604252A1 (en) Expression vectors for enhanced production of polypeptides, plasmids containing the vectors, hosts containing the plasmids, products manufactured thereby and related methods.
ES8207514A1 (en) Mature human leukocyte interferon A, process for its microbial preparation, intermediates therefor and compositions containing it.
ES8307297A1 (en) Polypeptides, process for their microbial production, intermediates therefor and compositions containing them.
ES8306797A1 (en) Bacillus cloning vectors, recombinant DNA molecules, bacillus hosts transformed with them and methods for expressing foreign DNA sequences and producing polypeptides coded thereby.
EP0068693A3 (en) Production of foot and mouth disease vaccine from microbially expressed antigens
DK163937B (en) Structural gene which encodes authentic human serum albumin for expression in a non-human host, process for preparing it, recombinant DNA molecule which contains the gene, host organism which is transformed with it, and process for preparing authentic human serum albumin
ES8603574A1 (en) Production of polypeptides with a human gamma-interferon activity.
HU209747B (en) Process for producing fusion proteins with eukaryotic ballastparts
EP0256424B1 (en) Homogeneous recombinant immune interferon fragments and pharmaceutical compositions containing same
DE3666838D1 (en) Plasmid vectors for expression in escherichia coli and/or bacillus subtilis
MX9701614A (en) Method for the production of recombinant proteins, plasmids and modified cells.
DE3572843D1 (en) Site-specific proteolysis by renin
EP0072541A3 (en) Human leukocyte interferons, process for their microbial production, intermediates therefor and compositions containing them
GR3020035T3 (en) Mammalian cell derived autonomously replicating sequence DNA fragments having affinity to DNA binding proteins
ATE5151T1 (en) MICROBIOLOGICALLY PRODUCED POLYPEPTIDE WITH THE AMINO ACID SEQUENCE OF MONKEY PROINSULIN, DNA AND PLASMIDS CODING FOR THIS SEQUENCE, MICROORGANISMS CONTAINING SUCH GENETIC INFORMATION AND METHODS OF PRODUCTION THEREOF.
Mashko et al. Use of a dual-origin temperature-controlled amplifiable replicon for optimization of human interleukin-1β synthesis in Escherichia coli
BG52178B2 (en) Method for chemical synthesis and expression of the gene of human noncystein -interferon

Legal Events

Date Code Title Description
RH Patent void