AU579991B2 - Nucleic acids, plasmids containing such acids, processes for their preparation and the use of such plasmids in the synthesis of gene products - Google Patents

Nucleic acids, plasmids containing such acids, processes for their preparation and the use of such plasmids in the synthesis of gene products

Info

Publication number
AU579991B2
AU579991B2 AU22832/83A AU2283283A AU579991B2 AU 579991 B2 AU579991 B2 AU 579991B2 AU 22832/83 A AU22832/83 A AU 22832/83A AU 2283283 A AU2283283 A AU 2283283A AU 579991 B2 AU579991 B2 AU 579991B2
Authority
AU
Australia
Prior art keywords
plasmids
acids
preparation
gene products
synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU22832/83A
Other versions
AU2283283A (en
Inventor
Gunther Adolf
Marc-Bruce Dworkin
Eva Dworkin-Rastl
Norbert Hauel
Rudolf Hauptmann
Peter Meindl
Peter Swetly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of AU2283283A publication Critical patent/AU2283283A/en
Application granted granted Critical
Publication of AU579991B2 publication Critical patent/AU579991B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

Abstract

The present invention relates to the combination of the promoter/operator region of the tryptophan operon of Serratia marcescens with a synthetic ribosome binding sequence, an expression plasmid which contains this sequence and in which the above sequence is followed by the only Hind III cleavage site in the plasmid, into which site the gene to be expressed can be inserted, and to production plasmids for the expression of eukaryotic gene products in prokaryotes, especially for the preparation of leucocyte interferon.
AU22832/83A 1982-12-24 1983-12-23 Nucleic acids, plasmids containing such acids, processes for their preparation and the use of such plasmids in the synthesis of gene products Ceased AU579991B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19823247922 DE3247922A1 (en) 1982-12-24 1982-12-24 DNA SEQUENCES, THEIR PRODUCTION, PLASMIDES CONTAINING THESE SEQUENCES AND THE USE THEREOF FOR THE SYNTHESIS OF EUKARYOTIC GENE PRODUCTS IN PROKARYOTS
DE3247922 1982-12-24

Publications (2)

Publication Number Publication Date
AU2283283A AU2283283A (en) 1984-06-28
AU579991B2 true AU579991B2 (en) 1988-12-22

Family

ID=6181687

Family Applications (1)

Application Number Title Priority Date Filing Date
AU22832/83A Ceased AU579991B2 (en) 1982-12-24 1983-12-23 Nucleic acids, plasmids containing such acids, processes for their preparation and the use of such plasmids in the synthesis of gene products

Country Status (19)

Country Link
EP (1) EP0115613B1 (en)
JP (2) JPH088868B2 (en)
KR (1) KR930005455B1 (en)
AT (1) ATE62018T1 (en)
AU (1) AU579991B2 (en)
DD (1) DD216043A5 (en)
DE (2) DE3247922A1 (en)
DK (1) DK576983A (en)
ES (4) ES8501798A1 (en)
FI (1) FI86991C (en)
GR (1) GR78766B (en)
HU (1) HU202277B (en)
IL (1) IL70523A (en)
MX (1) MX9202878A (en)
NO (1) NO173742C (en)
NZ (1) NZ206700A (en)
SU (2) SU1366064A3 (en)
UA (1) UA8035A1 (en)
ZA (1) ZA839519B (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3584198D1 (en) * 1984-08-01 1991-10-31 Boehringer Ingelheim Int NEW GENETIC SEQUENCES CODED BY TYPE I INTERFERON PEPTIDES AND THESE PRODUCING ORGANISMS.
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
HU204563B (en) * 1984-12-06 1992-01-28 Synergen Biolog Inc Process for producing recombinant serine-protease inhibitors and dns sequencyes for them
FI90990C (en) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Recombinant DNA molecule, transformed host organism, and method for producing interferon
DK151585D0 (en) * 1985-04-03 1985-04-03 Nordisk Gentofte DNA sequence
DK156072C (en) * 1985-04-03 1989-11-06 Nordisk Gentofte SYNTHETIC DNA SEQUENCE CONTAINING A RIBO BINDING SITE
DE3514113A1 (en) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt CHANGE OF THE DNA SEQUENCE BETWEEN SHINE-DALGARNO SEQUENCE AND START CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
DE3607835A1 (en) * 1986-03-10 1987-09-24 Boehringer Ingelheim Int HYBRID INTERFERONS, THEIR USE AS MEDICINAL PRODUCTS AND AS INTERMEDIATE PRODUCTS FOR THE PRODUCTION OF ANTIBODIES AND THE USE THEREOF AND METHOD FOR THEIR PRODUCTION
DE3642096A1 (en) * 1986-12-10 1988-06-16 Boehringer Ingelheim Int HORSE (GAMMA) INTERFERON
GB8813032D0 (en) * 1988-06-02 1988-07-06 Boehringer Ingelheim Int Antiviral pharmaceutical composition
AU4315089A (en) * 1988-11-04 1990-05-28 Upjohn Company, The Somatotropin expression using a serratia promoter
EP0626448A3 (en) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Process for preparing and purifying alpha-interferon
US5531880A (en) * 1994-09-13 1996-07-02 Microelectronics And Computer Technology Corporation Method for producing thin, uniform powder phosphor for display screens
FR2724665B1 (en) * 1994-09-16 1996-12-20 Rhone Poulenc Rorer Sa PROCESS FOR PRODUCING RECOMBINANT PROTEINS, PLASMIDS AND MODIFIED CELLS

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
EP0042246B1 (en) * 1980-06-12 1987-03-18 The Cancer Institute Of Japanese Foundation For Cancer Research Plasmid
ZA814375B (en) * 1980-07-01 1982-07-28 Hoffmann La Roche Interferons and process for their preparation
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0062971B1 (en) * 1981-03-27 1990-03-07 Imperial Chemical Industries Plc Genetically modified microorganisms
DE3220116A1 (en) * 1982-05-28 1983-12-01 Dr. Karl Thomae Gmbh, 7950 Biberach MICROBIOLOGICALLY MANUFACTURED (ALPHA) AND SS INTERFERONES, DNA SEQUENCES CODING FOR THESE INTERFERONES, MICROORGANISMS CONTAINING THIS GENETIC INFORMATION, AND METHOD FOR THE PRODUCTION THEREOF
DE3247923A1 (en) * 1982-12-24 1984-06-28 Dr. Karl Thomae Gmbh, 7950 Biberach Oligonucleotides and process for their preparation

Also Published As

Publication number Publication date
FI834700A (en) 1984-06-25
NZ206700A (en) 1987-11-27
EP0115613A3 (en) 1986-07-23
FI834700A0 (en) 1983-12-21
FI86991C (en) 1992-11-10
ES528350A0 (en) 1984-12-01
ZA839519B (en) 1985-08-28
JPH05214000A (en) 1993-08-24
ES8503372A1 (en) 1985-02-16
IL70523A (en) 1991-08-16
ES533213A0 (en) 1985-06-16
JPS59162888A (en) 1984-09-13
EP0115613A2 (en) 1984-08-15
GR78766B (en) 1984-10-02
MX9202878A (en) 1992-06-30
DE3382233D1 (en) 1991-05-02
ES8504930A1 (en) 1985-04-16
HU202277B (en) 1991-02-28
DK576983D0 (en) 1983-12-14
NO834792L (en) 1984-06-25
DE3247922A1 (en) 1984-06-28
UA8035A1 (en) 1995-12-26
DK576983A (en) 1984-06-25
KR840007105A (en) 1984-12-05
SU1366064A3 (en) 1988-01-07
JPH088868B2 (en) 1996-01-31
NO173742C (en) 1994-01-26
ES533197A0 (en) 1985-02-16
DD216043A5 (en) 1984-11-28
ES533214A0 (en) 1985-04-16
ES8501798A1 (en) 1984-12-01
SU1417800A3 (en) 1988-08-15
JP2637328B2 (en) 1997-08-06
NO173742B (en) 1993-10-18
ES8506090A1 (en) 1985-06-16
ATE62018T1 (en) 1991-04-15
AU2283283A (en) 1984-06-28
FI86991B (en) 1992-07-31
EP0115613B1 (en) 1991-03-27
KR930005455B1 (en) 1993-06-22
IL70523A0 (en) 1984-03-30

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