UA8035A1 - СПОСІБ ОДЕРЖАННЯ alРha-ІНТЕРФЕРОНА ЛЮДИНИ - Google Patents

СПОСІБ ОДЕРЖАННЯ alРha-ІНТЕРФЕРОНА ЛЮДИНИ

Info

Publication number
UA8035A1
UA8035A1 UA3681952A UA3681952A UA8035A1 UA 8035 A1 UA8035 A1 UA 8035A1 UA 3681952 A UA3681952 A UA 3681952A UA 3681952 A UA3681952 A UA 3681952A UA 8035 A1 UA8035 A1 UA 8035A1
Authority
UA
Ukraine
Prior art keywords
interferon alpha
human interferon
producing human
plasmid
engineering
Prior art date
Application number
UA3681952A
Other languages
English (en)
Russian (ru)
Inventor
Дворкін Марк-Брус
Дворкин Марк-Брус
Дворкін-Растль Ева
Адольф Гюнтер
Гауель Hорберт
Мейндль Петер
Светли Петер
Хауптманн Рудольф
Original Assignee
"Берінгер Інгельгейм Інтернаціональ Гмбх"
Берингер Ингельгейм Интернациональ ГмбХ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by "Берінгер Інгельгейм Інтернаціональ Гмбх", Берингер Ингельгейм Интернациональ ГмбХ filed Critical "Берінгер Інгельгейм Інтернаціональ Гмбх"
Publication of UA8035A1 publication Critical patent/UA8035A1/uk

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Спосіб одержання альфа-інтерферону людини передбачає конструювання рекомбінантної плазмідної ДНК, що містить ген альфа-інтерферону, трансформацію Escherichia coli даною плазмідою з подальшим культивуванням і виділенням кінцевого продукту. З метою підвищення виходу кінцевого продукту конструюється рекомбінантна плазмідна ДНК. Для цього в плазміду ВК322, розщеплену ендонуклеазами Eco RI і Hind III, вбудовується фрагмент певної нуклеотидної послідовності.
UA3681952A 1982-12-24 1983-12-23 СПОСІБ ОДЕРЖАННЯ alРha-ІНТЕРФЕРОНА ЛЮДИНИ UA8035A1 (uk)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19823247922 DE3247922A1 (de) 1982-12-24 1982-12-24 Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten

Publications (1)

Publication Number Publication Date
UA8035A1 true UA8035A1 (uk) 1995-12-26

Family

ID=6181687

Family Applications (1)

Application Number Title Priority Date Filing Date
UA3681952A UA8035A1 (uk) 1982-12-24 1983-12-23 СПОСІБ ОДЕРЖАННЯ alРha-ІНТЕРФЕРОНА ЛЮДИНИ

Country Status (19)

Country Link
EP (1) EP0115613B1 (uk)
JP (2) JPH088868B2 (uk)
KR (1) KR930005455B1 (uk)
AT (1) ATE62018T1 (uk)
AU (1) AU579991B2 (uk)
DD (1) DD216043A5 (uk)
DE (2) DE3247922A1 (uk)
DK (1) DK576983A (uk)
ES (4) ES8501798A1 (uk)
FI (1) FI86991C (uk)
GR (1) GR78766B (uk)
HU (1) HU202277B (uk)
IL (1) IL70523A (uk)
MX (1) MX9202878A (uk)
NO (1) NO173742C (uk)
NZ (1) NZ206700A (uk)
SU (2) SU1366064A3 (uk)
UA (1) UA8035A1 (uk)
ZA (1) ZA839519B (uk)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3584198D1 (de) * 1984-08-01 1991-10-31 Boehringer Ingelheim Int Neue genetische sequenzen, die durch sie codierten interferon-peptide vom typ i und diese sie produzierende organismen.
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
DE3587875T3 (de) * 1984-12-06 2003-01-02 Amgen Inc.(N.D.Ges D. Staates Delaware), Thousand Oaks Rekombinantverfahren zur herstellung von serinproteaseinhibitoren, sowie dns-sequenzen dazu.
FI90990C (fi) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Rekombinantti-DNA-molekyyli, transformoitu isäntäorganismi ja menetelmä interferonin valmistamiseksi
DK151585D0 (da) * 1985-04-03 1985-04-03 Nordisk Gentofte Dna-sekvens
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
DE3514113A1 (de) * 1985-04-19 1986-10-23 Hoechst Ag, 6230 Frankfurt Veraenderung der dna-sequenz zwischen shine-dalgarno-sequenz und startcodon des trp-operons zur steigerung der proteinexpression
US4874703A (en) * 1985-08-26 1989-10-17 Eli Lilly And Company Expression vectors for use in E. coli
DE3607835A1 (de) * 1986-03-10 1987-09-24 Boehringer Ingelheim Int Hybridinterferone, deren verwendung als arzneimittel und als zwischenprodukte zur herstellung von antikoerpern und deren verwendung sowie verfahren zu ihrer herstellung
DE3642096A1 (de) * 1986-12-10 1988-06-16 Boehringer Ingelheim Int Pferde-(gamma)-interferon
GB8813032D0 (en) * 1988-06-02 1988-07-06 Boehringer Ingelheim Int Antiviral pharmaceutical composition
AU4315089A (en) * 1988-11-04 1990-05-28 Upjohn Company, The Somatotropin expression using a serratia promoter
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
US5531880A (en) * 1994-09-13 1996-07-02 Microelectronics And Computer Technology Corporation Method for producing thin, uniform powder phosphor for display screens
FR2724665B1 (fr) * 1994-09-16 1996-12-20 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes, plasmides et cellules modifiees

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
EP0042246B1 (en) * 1980-06-12 1987-03-18 The Cancer Institute Of Japanese Foundation For Cancer Research Plasmid
ZA814375B (en) * 1980-07-01 1982-07-28 Hoffmann La Roche Interferons and process for their preparation
IE52755B1 (en) * 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0062971B1 (en) * 1981-03-27 1990-03-07 Imperial Chemical Industries Plc Genetically modified microorganisms
DE3220116A1 (de) * 1982-05-28 1983-12-01 Dr. Karl Thomae Gmbh, 7950 Biberach Mikrobiologisch hergestellte (alpha)- und ss-interferone, dna-sequenzen, die fuer diese interferone codieren, mikroorganismen, die diese genetische information enthalten, und verfahren zu ihrer herstellung
DE3247923A1 (de) * 1982-12-24 1984-06-28 Dr. Karl Thomae Gmbh, 7950 Biberach Neue oligonucleotide und verfahren zu ihrer herstellung

Also Published As

Publication number Publication date
MX9202878A (es) 1992-06-30
ES533213A0 (es) 1985-06-16
HU202277B (en) 1991-02-28
ES8504930A1 (es) 1985-04-16
ZA839519B (en) 1985-08-28
GR78766B (uk) 1984-10-02
EP0115613B1 (de) 1991-03-27
JPS59162888A (ja) 1984-09-13
SU1417800A3 (ru) 1988-08-15
ES528350A0 (es) 1984-12-01
DK576983D0 (da) 1983-12-14
NO834792L (no) 1984-06-25
JPH05214000A (ja) 1993-08-24
KR930005455B1 (ko) 1993-06-22
FI834700A0 (fi) 1983-12-21
ES533197A0 (es) 1985-02-16
IL70523A0 (en) 1984-03-30
DD216043A5 (de) 1984-11-28
EP0115613A3 (en) 1986-07-23
ATE62018T1 (de) 1991-04-15
ES8503372A1 (es) 1985-02-16
DE3247922A1 (de) 1984-06-28
FI86991C (fi) 1992-11-10
NZ206700A (en) 1987-11-27
FI86991B (fi) 1992-07-31
ES8506090A1 (es) 1985-06-16
ES533214A0 (es) 1985-04-16
JPH088868B2 (ja) 1996-01-31
IL70523A (en) 1991-08-16
ES8501798A1 (es) 1984-12-01
AU2283283A (en) 1984-06-28
NO173742C (no) 1994-01-26
SU1366064A3 (ru) 1988-01-07
DK576983A (da) 1984-06-25
FI834700A (fi) 1984-06-25
EP0115613A2 (de) 1984-08-15
KR840007105A (ko) 1984-12-05
JP2637328B2 (ja) 1997-08-06
AU579991B2 (en) 1988-12-22
NO173742B (no) 1993-10-18
DE3382233D1 (de) 1991-05-02

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