ES2062757T3 - Vector de clonacion para uso en bacterias de acido lactico y un metodo para construirlo. - Google Patents

Vector de clonacion para uso en bacterias de acido lactico y un metodo para construirlo.

Info

Publication number
ES2062757T3
ES2062757T3 ES91901706T ES91901706T ES2062757T3 ES 2062757 T3 ES2062757 T3 ES 2062757T3 ES 91901706 T ES91901706 T ES 91901706T ES 91901706 T ES91901706 T ES 91901706T ES 2062757 T3 ES2062757 T3 ES 2062757T3
Authority
ES
Spain
Prior art keywords
lactic acid
pct
recombinant plasmid
cloning vector
acid bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91901706T
Other languages
English (en)
Inventor
Stephen Wessels
Jytte Josephsen
Finn Vogensen
Egil Waagner Nielsen
Wright Atte Von
Soile Tynkkynen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valio Finnish Cooperative Dairies Association
Original Assignee
Valio Finnish Cooperative Dairies Association
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valio Finnish Cooperative Dairies Association filed Critical Valio Finnish Cooperative Dairies Association
Application granted granted Critical
Publication of ES2062757T3 publication Critical patent/ES2062757T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/335Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

VECTOR DE CLONACION PARA UTILIZARLO EN BACTERIAS DE ACIDO LACTICO Y METODO PARA LA FORMACION DEL MISMO SE PRESENTAN UN PLASMIDO RECOMBINANTE UTIL COMO VECTOR DE CLONACION EN BACTERIAS DE ACIDO LACTICO Y QUE SOLO CONTIENE ADN DE ORIGEN BACTERIANO DE ACIDO LACTICO, CONSTANDO EL PLASMIDO DE (A) UN FRAGMENTO DE ADN QUE CONTIENE UNA REGION DE REPRODUCCION FUNCIONAL EN BACTERIAS DE ACIDO LACTICO Y (B) UN FRAGMENTO DE ADN QUE CONTIENE UN GEN MARCADOR SELECCIONABLE EN LA BACTERIA LACTICA, CUYA EXPRESION PERMITE LA SELECCION PRIMARIA EN UN SOLO PASO EN LAS CELULAS BACTERIANAS DE ACIDO LACTICO TRANSFORMADAS CON EL PLASMIDO RECOMBINANTE; UN METODO PARA LA FORMACION DEL PLASMIDO DEL VECTOR DE CLONACION; EL PLASMIDO RECOMBINANTE CON UN GEN INSERTADO QUE CODIFICA UN PRODUCTO GENETICO DESEADO; UN METODO PARA LA PREPARACION DE UN CULTIVO INICIADOR DE ALIMENTOS MEJORADO A BASE DE INTRODUCIR EL PLASMIDO RECOMBINANTE CON UN GEN INSERTADO QUE CODIFICA UN PRODUCTO GENETICO DESEADO Y CULTIVOS INICIADORES DE ALIMENTOS PREPARADOS SEGUN EL METODO.
ES91901706T 1989-12-20 1990-12-20 Vector de clonacion para uso en bacterias de acido lactico y un metodo para construirlo. Expired - Lifetime ES2062757T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/DK1989/000298 WO1991009131A1 (en) 1989-12-20 1989-12-20 Cloning vector for use in lactic acid bacteria

Publications (1)

Publication Number Publication Date
ES2062757T3 true ES2062757T3 (es) 1994-12-16

Family

ID=8153564

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91901706T Expired - Lifetime ES2062757T3 (es) 1989-12-20 1990-12-20 Vector de clonacion para uso en bacterias de acido lactico y un metodo para construirlo.

Country Status (10)

Country Link
US (1) US5580787A (es)
EP (1) EP0506789B1 (es)
AT (1) ATE102652T1 (es)
CA (1) CA2072007C (es)
DE (1) DE69007298T2 (es)
DK (1) DK0506789T3 (es)
ES (1) ES2062757T3 (es)
IE (1) IE66032B1 (es)
NZ (1) NZ236570A (es)
WO (2) WO1991009131A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPM876494A0 (en) * 1994-10-13 1994-11-03 Mauri Laboratories Pty Ltd Plasmids encoding bacteriophage resistance for use in lactic acid bacteria
GB9512106D0 (en) * 1995-06-14 1995-08-09 Norsk Naeringsmiddelforskning Animal feed
BR9809197B1 (pt) * 1997-05-30 2013-09-03 método de aumento da taxa de crescimento e/ou de controle da atividade metabólica de uma cepa bacteriana de ácido lático e composição de cultura de iniciador.
AU743215B2 (en) * 1998-04-21 2002-01-24 Chr. Hansen A/S Food-grade cloning vector and their use in lactic acid bacteria
US7358083B1 (en) 1998-04-21 2008-04-15 Chr. Hansen A/S Food-grade cloning vector and their use in lactic acid bacteria
WO2002002747A2 (en) * 2000-07-05 2002-01-10 Danmarks Tekniske Universitet Method of improving biomass yield of lactic acid bacterial cultures
KR20020090546A (ko) * 2001-05-28 2002-12-05 주식회사 알엔에이 유산균 염색체 dna로의 삽입을 위한 삽입 벡터
EP1634948A1 (en) 2004-09-10 2006-03-15 Basf Aktiengesellschaft Means and methods for preventing and/or treating caries
CN100350049C (zh) * 2004-12-09 2007-11-21 中国疾病预防控制中心传染病预防控制所 乳酸乳球菌的食品级载体
US8557313B2 (en) * 2007-07-03 2013-10-15 Danmarks Tekniske Universitet Phage resistance
CA2721653C (en) * 2008-04-17 2018-03-20 Anaeropharma Science, Inc. Expression vector
EP2133414A1 (en) 2008-06-11 2009-12-16 Basf Se Uses and methods for preventing and /or treating oral malodour
WO2010139195A1 (en) * 2009-06-04 2010-12-09 The Chinese University Of Hong Kong Anti-inflammatory bacteria
BR112013016493A2 (pt) 2011-01-24 2016-09-27 Basf Se uso de um microrganismo com capacidade de ligação ou fragmento deste, método de preparar uma composição, composição, e, microrganismo com capacidade de ligação ou fragmento deste
ES2710435T3 (es) 2013-03-13 2019-04-25 Novozymes As Nuevas cepas de Lactobacillus y sus usos
FI126681B (en) * 2014-12-19 2017-03-31 Teknologian Tutkimuskeskus Vtt Oy Process for converting berry and fruit material into antimicrobially active fractions

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6030686A (ja) * 1983-07-30 1985-02-16 Yakult Honsha Co Ltd 乳酸桿菌属の細菌に供与dνaを導入する方法
US4918014A (en) * 1985-10-11 1990-04-17 Microlife Technics, Inc. Method for producing mucoid and phage resistant group N streptococcus strains from non-mucoid and phage sensitive parent strains
NL8503316A (nl) * 1985-11-29 1987-06-16 Stichting Nl I Zuivelonderzoek Werkwijze voor het bereiden van proteinen met behulp van gastheer-organismen, in het bijzonder melkzuurbacterien, welke recombinant dna-plasmiden met een hoge replicon-aktiviteit en een hoge promotor-aktiviteit voor het betreffende proteine-coderende gen bevatten, de van dergelijke recombinant dna-plasmiden voorziene gastheerorganismen, de recombinant dna-plasmiden zelve, de replicon-aktiviteit en promotor-aktiviteit coderende dna-fragmenten, alsmede de verkregen proteinen.
FI77056C (fi) * 1986-06-24 1989-01-10 Valio Meijerien Vektorplasmid som laempar sig foer syrningsmedel, speciellt foer mejerisyrningsmedel.
EP0311469A3 (en) * 1987-09-02 1990-05-30 Plant Genetic Systems N.V. Transformed lactic acid bacteria
EP0316677A3 (en) * 1987-11-13 1990-04-25 Miles Inc. Method for cloning in lactic acid bacteria

Also Published As

Publication number Publication date
AU645459B2 (en) 1994-01-13
DK0506789T3 (da) 1994-06-06
DE69007298T2 (de) 1994-06-30
NZ236570A (en) 1991-11-26
AU7045991A (en) 1991-07-18
DE69007298D1 (de) 1994-04-14
EP0506789B1 (en) 1994-03-09
WO1991009131A1 (en) 1991-06-27
EP0506789A1 (en) 1992-10-07
IE66032B1 (en) 1995-11-29
CA2072007C (en) 1999-11-16
CA2072007A1 (en) 1991-06-21
WO1991009132A1 (en) 1991-06-27
US5580787A (en) 1996-12-03
ATE102652T1 (de) 1994-03-15
IE904621A1 (en) 1991-07-17

Similar Documents

Publication Publication Date Title
ES2062757T3 (es) Vector de clonacion para uso en bacterias de acido lactico y un metodo para construirlo.
Garrett et al. Cell lysis by induction of cloned lambda lysis genes
CA1195626A (en) Recombinant dna cloning vectors and the eukaryotic and prokaryotic transformants thereof
ES8205265A1 (es) Un metodo para producir la hormona de crecimiento humano
EP0088195A3 (en) Messenger rna, production and use thereof
EP0143834A1 (en) Heat regulated production of selected and fused proteins in yeast
Chiba et al. Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors
CN101978057B (zh) 修饰宿主dna中的对象区域的方法和选择性标记盒
CN102453716A (zh) 猪骨骼肌特异性表达基因α-actin启动子的克隆及应用
ES8301503A1 (es) Procedimiento para la produccion de un microorganismo gene- ticamente modificado
UA8035A1 (uk) СПОСІБ ОДЕРЖАННЯ alРha-ІНТЕРФЕРОНА ЛЮДИНИ
Flavell Mitochondria and chloroplasts as descendants of prokaryotes
AU4113597A (en) A method of improving the production of biomass or a desired product from a cell
JO1694B1 (en) New chemical products
Wilson et al. Genetic mapping of a linked cluster of ribosomal ribonucleic acid genes in Bacillus subtilis
AU589204B2 (en) Stabilization of unstably inherited replicons
EP0133756A2 (en) Vector for expression of polypeptides
DK619284D0 (da) Udtrykkelsesplasmid til forbedret produktion af heterologt protein ibakterier og fremgangsmaade til fremstilling af dette protein samtfremgangsmaade til fremstilling af plasmidet
PT1144656E (pt) Processo para o isolamento do adn do plasmideo ccc
AU2002314183A1 (en) Method of plasmid stabilisation by in vivo deleting the antibiotic resistence and selecting with an essential gene
EP0135045A2 (en) A novel plasmid and methods for its use
EP0316677A3 (en) Method for cloning in lactic acid bacteria
NZ214549A (en) Expression control dna sequences
DE3586410T2 (de) Verfahren zur herstellung von neutraler protease.
DK0759981T3 (da) Fremgangsmåde til dyrkning af bakterier, der producerer proteiner, som udtrykkes på en temperaturreguleret måde

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 506789

Country of ref document: ES