ES2395898T3 - Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas - Google Patents
Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas Download PDFInfo
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- ES2395898T3 ES2395898T3 ES03747055T ES03747055T ES2395898T3 ES 2395898 T3 ES2395898 T3 ES 2395898T3 ES 03747055 T ES03747055 T ES 03747055T ES 03747055 T ES03747055 T ES 03747055T ES 2395898 T3 ES2395898 T3 ES 2395898T3
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| US37431302P | 2002-04-19 | 2002-04-19 | |
| PCT/US2003/012556 WO2003089620A2 (en) | 2002-04-19 | 2003-04-21 | Phospholipases, nucleic acids encoding them and methods for making and using them |
| US374313P | 2010-08-17 |
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| ES2395898T3 true ES2395898T3 (es) | 2013-02-15 |
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| ES (1) | ES2395898T3 (https=) |
| PT (1) | PT1497418E (https=) |
| WO (1) | WO2003089620A2 (https=) |
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| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| ES2284897T3 (es) | 2001-05-18 | 2007-11-16 | Danisco A/S | Procedimiento para la preparacion de una masa con una enzima. |
| US7226771B2 (en) * | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| US7943360B2 (en) | 2002-04-19 | 2011-05-17 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| EP2853593B1 (en) | 2003-03-07 | 2017-10-04 | DSM IP Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
| DE10355838A1 (de) * | 2003-11-26 | 2005-07-07 | Martin-Luther-Universität Halle-Wittenberg | Verfahren zur Herstellung von Phospholipase A2 |
| AU2011218662B2 (en) * | 2004-03-08 | 2013-01-17 | Verenium Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| CN101432292B (zh) * | 2004-06-16 | 2013-03-13 | 维莱尼姆公司 | 对叶绿素进行酶促脱色的组合物和方法 |
| US20080070291A1 (en) | 2004-06-16 | 2008-03-20 | David Lam | Compositions and Methods for Enzymatic Decolorization of Chlorophyll |
| CN102533440B (zh) | 2004-07-16 | 2017-09-19 | 杜邦营养生物科学有限公司 | 用酶使油脱胶的方法 |
| US8557551B2 (en) | 2004-09-10 | 2013-10-15 | Dsm Ip Assets B.V. | Compositions and methods for making and modifying oils |
| CA2611071A1 (en) * | 2005-05-31 | 2007-05-18 | Verenium Corporation | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US20070148311A1 (en) * | 2005-12-22 | 2007-06-28 | Bunge Oils, Inc. | Phytosterol esterification product and method of make same |
| US20090324574A1 (en) * | 2006-02-02 | 2009-12-31 | Verenium Corporation | Esterases and Related Nucleic Acids and Methods |
| AR062947A1 (es) * | 2006-09-21 | 2008-12-17 | Verenium Corp | Fosfolipasas acidos nucleicos que las codifican y metodos para prepararlas y usarlas |
| US20080235824A1 (en) * | 2006-12-17 | 2008-09-25 | Gabriel Dean W | Use of Esterase Genes as Selectable Markers for Transforming Plant Cells |
| CN101610824A (zh) * | 2006-12-22 | 2009-12-23 | 丹尼斯科美国公司 | 酶辅助的水性脂类提取物的脱乳化作用 |
| US8460905B2 (en) * | 2007-09-11 | 2013-06-11 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time |
| US8956853B2 (en) * | 2007-01-30 | 2015-02-17 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases |
| ITRM20070129A1 (it) * | 2007-03-14 | 2008-09-15 | Plantechno S R L | Piante di tabacco mutagenizzate come coltura da seme per la produzioe di olio da usare ai fini energetici industriali e alimentari |
| JP5302189B2 (ja) * | 2007-04-27 | 2013-10-02 | 長瀬産業株式会社 | スフィンゴミエリナーゼ |
| US20090064567A1 (en) | 2007-09-12 | 2009-03-12 | Martek Biosciences Corporation | Biological oils and production and uses Thereof |
| US8076123B2 (en) * | 2007-10-26 | 2011-12-13 | Oilseeds Biorefinery Corporation | Emulsification-free degumming of oil |
| WO2009081094A2 (en) * | 2007-12-21 | 2009-07-02 | Danisco A/S | Process |
| US8241876B2 (en) | 2008-01-07 | 2012-08-14 | Bunge Oils, Inc. | Generation of triacylglycerols from gums |
| UA111708C2 (uk) * | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
| CN103068965A (zh) | 2009-12-28 | 2013-04-24 | Dsmip资产公司 | 在蔗糖上生长的重组破囊壶菌和其组合物、制备方法及用途 |
| ES2651313T3 (es) * | 2009-12-28 | 2018-01-25 | Dsm Ip Assets B.V. | Traustoquítridos recombinantes que crecen en xilosa, y composiciones, métodos de preparación y usos de los mismos |
| MX2012010399A (es) | 2010-03-12 | 2012-10-05 | Dupont Nutrition Biosci Aps | Proceso. |
| US20130085287A1 (en) | 2010-06-17 | 2013-04-04 | Dupont Nutrition Biosciences Aps | Process |
| KR101202135B1 (ko) | 2010-06-22 | 2012-11-15 | 고려대학교 산학협력단 | 병저항성 관련 고추 파타틴 유사 포스포라이페이즈 유전자 ＣａＰＬＰ１ 및 이를 이용한 식물병 저항성 탐색 및 형질전환 식물체 |
| DE102010025764A1 (de) * | 2010-07-01 | 2012-01-05 | Süd-Chemie AG | Phospholipase-Träger-Komplex |
| AR085251A1 (es) | 2011-02-23 | 2013-09-18 | Danisco | Proceso para tratar aceite vegetal |
| WO2012114234A1 (en) | 2011-02-23 | 2012-08-30 | Dupont Nutrition Biosciences Aps | Process |
| CN102504946B (zh) * | 2011-11-04 | 2013-09-04 | 福建春辉生物工程有限公司 | 一种对三高人群有益的油茶籽毛油精炼方法 |
| WO2013104660A1 (en) | 2012-01-13 | 2013-07-18 | Dupont Nutrition Biosciences Aps | Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation |
| WO2013160372A1 (en) | 2012-04-27 | 2013-10-31 | Dupont Nutrition Biosciences Aps | Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme |
| RU2500811C1 (ru) * | 2012-10-10 | 2013-12-10 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") | Рекомбинантный штамм бактерий bacillus subtilis - продуцент фосфолипазы с |
| WO2014160862A1 (en) | 2013-03-27 | 2014-10-02 | The Curators Of The University Of Missouri | Increasing the content of long chain fatty acids in seed oil |
| CN103540576B (zh) * | 2013-09-30 | 2015-05-20 | 上海交通大学 | patatin-like磷脂酶及其编码基因与用途 |
| CN104630176B (zh) * | 2013-11-13 | 2020-05-01 | 丰益(上海)生物技术研发中心有限公司 | 一种提高发酵法生产磷脂酶c产量的方法 |
| CN104726357B (zh) * | 2013-12-20 | 2019-07-09 | 丰益(上海)生物技术研发中心有限公司 | 一株产生磷脂酶的解淀粉芽孢杆菌诱变菌株及磷脂酶c |
| BR112016021474B1 (pt) | 2014-03-19 | 2022-10-04 | Novozymes A/S | Composição compreendendo uma fosfatidilinositol fosfolipase c e um polipeptídeo de fosfolipase c específica para fosfatidilcolina e fosfatidiletanolamina e método para reduzir o teor de fosfolipídeos em uma composição de óleo |
| US20170058235A1 (en) | 2014-05-15 | 2017-03-02 | Novozymes A/S | Compositions Comprising Polypeptides Having Phospholipase C Activity and Use Thereof |
| CN104604704A (zh) * | 2015-02-16 | 2015-05-13 | 安徽科技学院 | 一种b5(含碳源)固体培养基质的规模化制作方法 |
| EP3282856A1 (en) | 2015-04-14 | 2018-02-21 | DSM IP Assets B.V. | Use of phospholipase c |
| CA3014326C (en) | 2016-02-19 | 2024-01-09 | Basf Se | Baking lipases |
| WO2017151103A1 (en) * | 2016-02-29 | 2017-09-08 | Hewlett-Packard Development Company, L.P. | Enzymatic sample purification |
| CN105567713B (zh) * | 2016-03-09 | 2019-01-08 | 河北省农林科学院粮油作物研究所 | 花生AhPLDα3蛋白及其编码基因与应用 |
| CN110218712B (zh) * | 2016-06-02 | 2021-08-03 | 天津科技大学 | 磷脂酶d突变体及其在制备磷脂酸、磷脂酰丝氨酸中的应用 |
| CN108220267B (zh) * | 2016-12-22 | 2022-10-04 | 丰益(上海)生物技术研发中心有限公司 | 磷脂酶及其应用 |
| RU2019140871A (ru) | 2017-05-12 | 2021-06-16 | Басф Се | Способ применения липазных ферментов для очистки |
| US12110531B2 (en) | 2017-06-16 | 2024-10-08 | Danstar Ferment Ag | Method for increasing oil yield during ethanol production |
| CA3076578A1 (en) | 2017-10-25 | 2019-05-02 | Basf Se | Beta-amylase enzymes |
| CN108384768A (zh) * | 2018-02-28 | 2018-08-10 | 江苏中酶生物科技有限公司 | 一种磷脂酶c的突变体及其应用 |
| US11732250B2 (en) | 2018-04-26 | 2023-08-22 | Basf Se | Lipase enzymes |
| US20210115422A1 (en) | 2018-05-03 | 2021-04-22 | Basf Se | Amylase enzymes |
| CN111378634B (zh) * | 2018-12-28 | 2024-04-02 | 丰益(上海)生物技术研发中心有限公司 | 具有磷脂酶c活性的多肽及其用途 |
| CN111051505B (zh) * | 2018-12-29 | 2023-11-03 | 邦泰生物工程(深圳)有限公司 | 一种磷脂酶d突变体、其应用及其制备磷酯酰丝氨酸的方法 |
| US20220186177A1 (en) | 2019-02-20 | 2022-06-16 | Basf Se | Industrial fermentation process for bacillus using defined medium and magnesium feed |
| MX2021010109A (es) | 2019-02-20 | 2021-09-21 | Basf Se | Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos. |
| WO2020185737A1 (en) | 2019-03-11 | 2020-09-17 | Basf Se | Amylases and methods for making and using them |
| WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
| US12606809B2 (en) | 2019-03-25 | 2026-04-21 | Basf Se | Amylase enzymes |
| US20220162576A1 (en) | 2019-03-25 | 2022-05-26 | Basf Se | Amylase enzymes |
| MX2021015364A (es) | 2019-06-13 | 2022-01-24 | Basf Se | Metodo de recuperacion de una proteina de un caldo de fermentacion mediante el uso de un cation divalente. |
| PL3959326T3 (pl) | 2019-07-05 | 2023-10-09 | Basf Se | Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem |
| CN114364795A (zh) | 2019-08-22 | 2022-04-15 | 巴斯夫欧洲公司 | 淀粉酶变体 |
| CN119630280A (zh) | 2022-05-14 | 2025-03-14 | 诺维信公司 | 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法 |
| CN114835780B (zh) * | 2022-06-29 | 2022-09-27 | 中国农业大学 | 辣椒籽分离的寡肽medei及其在预防或治疗癌症中的应用 |
| CN116790552B (zh) * | 2022-11-28 | 2024-11-01 | 柏定生物工程(北京)有限公司 | 鞘磷脂酶突变体及其在sdLDL胆固醇检测中的应用 |
| CN120640986A (zh) | 2023-02-24 | 2025-09-12 | 卡拉万成分股份有限公司 | 蛋类替代物组合物及其制备和使用方法 |
| WO2024243330A1 (en) | 2023-05-22 | 2024-11-28 | Caravan Ingredients Inc. | Baked goods enhancer and methods of making and using the same |
Family Cites Families (129)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US87571A (en) | 1869-03-09 | Improved axle-set | ||
| SE318048B (https=) | 1959-09-21 | 1969-12-01 | Pellerin Ab Zenith | |
| SE417441B (sv) | 1977-08-19 | 1981-03-16 | Kare Larsson | Forfarande for att isolera polera lipider ur en blandning av polera och opolera cerealiclipider genom blandning med vatten och derpa foljande tyngdkraftsseparation |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4707364A (en) * | 1984-01-27 | 1987-11-17 | Miles Laboratories, Inc. | Composition for accelerating cheese aging |
| US5087571A (en) | 1984-06-22 | 1992-02-11 | President And Fellows Of Harvard College | Method for providing a cell culture from a transgenic non-human mammal |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| GB2183661B (en) | 1985-03-30 | 1989-06-28 | Marc Ballivet | Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique |
| US4752483A (en) * | 1986-05-30 | 1988-06-21 | Campbell Soup Company | Method for producing a highly flavored cheese ingredient |
| US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| US5573933A (en) | 1987-04-14 | 1996-11-12 | Luminis Pty, Ltd. | Transgenic pigs |
| US6054270A (en) | 1988-05-03 | 2000-04-25 | Oxford Gene Technology Limited | Analying polynucleotide sequences |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| US5217879A (en) | 1989-01-12 | 1993-06-08 | Washington University | Infectious Sindbis virus vectors |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5527681A (en) | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5288619A (en) | 1989-12-18 | 1994-02-22 | Kraft General Foods, Inc. | Enzymatic method for preparing transesterified oils |
| WO1991016427A1 (en) | 1990-04-24 | 1991-10-31 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US5326477A (en) | 1990-05-07 | 1994-07-05 | Bio-Sep, Inc. | Process for digesting solid waste |
| US5387742A (en) | 1990-06-15 | 1995-02-07 | Scios Nova Inc. | Transgenic mice displaying the amyloid-forming pathology of alzheimer's disease |
| DE69133512T2 (de) | 1990-11-23 | 2006-09-28 | Bayer Bioscience N.V. | Verfahren zur Transformation monokotyler Pflanzen |
| EP0563296B1 (en) | 1990-12-20 | 1999-03-17 | Ixsys, Inc. | Optimization of binding proteins |
| US6110700A (en) | 1991-03-11 | 2000-08-29 | The General Hospital Corporation | PRAD1 cyclin and its cDNA |
| DE4115938A1 (de) | 1991-05-16 | 1992-11-19 | Metallgesellschaft Ag | Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen |
| US5922854A (en) | 1991-06-14 | 1999-07-13 | Kumar; Ramesh | Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids |
| CA2075135A1 (en) | 1991-08-02 | 1993-02-03 | David E. Ellis | Particle-mediated transformation of gymnosperms |
| US5632957A (en) | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
| JP2626380B2 (ja) | 1991-11-12 | 1997-07-02 | 株式会社大林組 | クレーン用吊治具の旋回防止装置 |
| JPH05236997A (ja) | 1992-02-28 | 1993-09-17 | Hitachi Ltd | ポリヌクレオチド捕捉用チップ |
| JP2937746B2 (ja) | 1993-04-25 | 1999-08-23 | 昭和産業株式会社 | 油脂の精製方法 |
| JPH06306386A (ja) | 1993-04-25 | 1994-11-01 | Showa Sangyo Co Ltd | 油脂の精製方法 |
| EP0670670A4 (en) | 1993-09-30 | 1996-04-24 | Agracetus | HETEROLOGICAL PEROXIDASE PROCESSING TRANSGENIC COTTON PLANTS. |
| US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
| US6468742B2 (en) | 1993-11-01 | 2002-10-22 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip |
| US5965452A (en) | 1996-07-09 | 1999-10-12 | Nanogen, Inc. | Multiplexed active biologic array |
| DE4339556C1 (de) | 1993-11-19 | 1995-02-02 | Metallgesellschaft Ag | Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5639940A (en) | 1994-03-03 | 1997-06-17 | Pharmaceutical Proteins Ltd. | Production of fibrinogen in transgenic animals |
| WO1995024494A1 (en) | 1994-03-09 | 1995-09-14 | Abbott Laboratories | Humanized milk |
| MX198456B (es) | 1994-03-09 | 2000-09-05 | Abbott Lab | Animales trangenicos que producen oligosacaridos y glucoconjugados. |
| DK0753057T3 (da) | 1994-03-29 | 2006-01-30 | Novozymes As | Alkalisk Bacillus-amylase |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5959171A (en) | 1994-08-17 | 1999-09-28 | Pharming B.V. | Method for the production of biologically active polypeptides in a mammal's |
| EP0778886A4 (en) | 1994-09-01 | 2001-05-02 | TRANSGENIC ANIMAL WHICH EXPRESSES A FAMILY FORM OF THE HUMAN AMYLOID FOREQUARTER PROTEIN | |
| US6111166A (en) | 1994-09-19 | 2000-08-29 | Medarex, Incorporated | Transgenic mice expressing human Fcα and β receptors |
| US5795737A (en) | 1994-09-19 | 1998-08-18 | The General Hospital Corporation | High level expression of proteins |
| US5880327A (en) | 1994-09-21 | 1999-03-09 | American National Red Cross | Transgenic mammals expressing human coagulation factor VIII |
| US5556752A (en) | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
| US6187992B1 (en) | 1994-12-05 | 2001-02-13 | Merck & Co., Inc. | Transgenic mouse having a disrupted amyloid precursor protein gene |
| US5830645A (en) | 1994-12-09 | 1998-11-03 | The Regents Of The University Of California | Comparative fluorescence hybridization to nucleic acid arrays |
| US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| US5959098A (en) | 1996-04-17 | 1999-09-28 | Affymetrix, Inc. | Substrate preparation process |
| CA2223103A1 (en) | 1995-06-06 | 1996-12-12 | Isis Pharmaceuticals Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
| TR199701705T1 (xx) | 1995-06-27 | 1998-04-21 | Unilever N.V. | Trigliserid ya�lar�n i�lenmesinde hareketsiz enzim ve kullan�m�. |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US5985662A (en) | 1995-07-13 | 1999-11-16 | Isis Pharmaceuticals Inc. | Antisense inhibition of hepatitis B virus replication |
| US5958672A (en) | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
| DE19527274A1 (de) | 1995-07-26 | 1997-01-30 | Metallgesellschaft Ag | Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase |
| SE504664C2 (sv) | 1995-09-22 | 1997-03-24 | Scotia Lipidteknik Ab | Sätt att framställa fraktionerad olja, oljan, dess användning samt emulsionskomposition innehållande oljan |
| US5721118A (en) | 1995-10-31 | 1998-02-24 | The Regents Of The University Of California, San Diego | Mammalian artificial chromosomes and methods of using same |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6022963A (en) | 1995-12-15 | 2000-02-08 | Affymetrix, Inc. | Synthesis of oligonucleotide arrays using photocleavable protecting groups |
| US6013440A (en) | 1996-03-11 | 2000-01-11 | Affymetrix, Inc. | Nucleic acid affinity columns |
| US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
| US6025155A (en) | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| US6197070B1 (en) | 1996-05-15 | 2001-03-06 | The Procter & Gamble Company | Detergent compositions comprising alpha combination of α-amylases for malodor stripping |
| AU3568897A (en) | 1996-06-07 | 1998-01-05 | Eos Biotechnology, Inc. | Immobilised linear oligonucleotide arrays |
| US6326341B1 (en) | 1996-09-11 | 2001-12-04 | The Procter & Gamble Company | Low foaming automatic dishwashing compositions |
| US6127137A (en) | 1996-10-31 | 2000-10-03 | Novo Nordisk A/S | Acidic phospholipase, production and methods using thereof |
| AU4772597A (en) | 1996-10-31 | 1998-05-22 | Novo Nordisk A/S | Novel phospholipase, production and use thereof |
| AU5187898A (en) | 1996-12-09 | 1998-07-03 | Novo Nordisk A/S | Reduction of phosphorus containing components in edible oils comprising a high amount of non-hydratable phosphorus by use of a phospholipase, a phospholipase from a filamentous fungus having phospholipase a and/or b activity |
| US6103505A (en) | 1996-12-09 | 2000-08-15 | Novo Nordisk A/S | Method for reducing phosphorus content of edible oils |
| JP4017082B2 (ja) | 1996-12-19 | 2007-12-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 固定化酵素及びトリグリセリド油の処理のためのその使用 |
| US6069122A (en) | 1997-06-16 | 2000-05-30 | The Procter & Gamble Company | Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| EP1717322B1 (en) | 1997-01-17 | 2012-07-18 | Codexis Mayflower Holdings, LLC | Evolution of whole cells and organisms by recursive sequence recombination |
| DE19703364A1 (de) | 1997-01-30 | 1998-08-06 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Wasch- und Reinigungsmittel |
| US6303803B1 (en) | 1997-01-31 | 2001-10-16 | Cargill, Incorporated | Removal of sterols from fats and oils |
| US5880300A (en) | 1997-01-31 | 1999-03-09 | Cargill, Incorporated | Phospholipid-based removal of sterols from fats and oils |
| EP0973940B1 (en) | 1997-03-18 | 2008-07-02 | Novozymes A/S | An in vitro method for the construction of a dna library |
| JP4263248B2 (ja) | 1997-03-18 | 2009-05-13 | ノボザイムス アクティーゼルスカブ | Ｄｎａのシャッフリングによるライブラリーの作成方法 |
| US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
| US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| US6826296B2 (en) | 1997-07-25 | 2004-11-30 | Affymetrix, Inc. | Method and system for providing a probe array chip design database |
| CN1273609A (zh) | 1997-08-15 | 2000-11-15 | 希斯克有限公司 | 检测或量化核酸物类的方法和组合物 |
| CN1677076A (zh) | 1997-09-11 | 2005-10-05 | 生物风险公司 | 制备高密度阵列的方法 |
| US6465178B2 (en) | 1997-09-30 | 2002-10-15 | Surmodics, Inc. | Target molecule attachment to surfaces |
| AU1124499A (en) | 1997-10-28 | 1999-05-17 | Maxygen, Inc. | Human papillomavirus vectors |
| CA2308119C (en) | 1997-10-30 | 2014-06-03 | Novo Nordisk A/S | .alpha.-amylase mutants |
| WO1999023107A1 (en) | 1997-10-31 | 1999-05-14 | Maxygen, Incorporated | Modification of virus tropism and host range by viral genome shuffling |
| EP1032644B1 (en) | 1997-11-10 | 2003-03-19 | The Procter & Gamble Company | Process for preparing a detergent tablet |
| US6118044A (en) | 1997-11-14 | 2000-09-12 | Sankyo Company, Limited | Transgenic animal allergy models and methods for their use |
| DK1036198T3 (da) | 1997-12-08 | 2013-01-02 | California Inst Of Techn | Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser |
| US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
| MXPA00007893A (es) | 1998-02-11 | 2002-10-23 | Maxygen Inc | Direccion de vectores de vacunas geneticas. |
| CA2320958A1 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Antigen library immunization |
| AU3463699A (en) | 1998-04-03 | 1999-10-25 | Phylos, Inc. | Addressable protein arrays |
| US6156952A (en) | 1998-04-09 | 2000-12-05 | Constituent Institution Of The University Of Maryland System | HIV transgenic animals and uses therefor |
| AU3408199A (en) | 1998-05-01 | 1999-11-23 | Novo Nordisk A/S | Enhancers such as n-hydroxyacetanilide |
| US6048695A (en) | 1998-05-04 | 2000-04-11 | Baylor College Of Medicine | Chemically modified nucleic acids and methods for coupling nucleic acids to solid support |
| DE19824705A1 (de) | 1998-06-03 | 1999-12-09 | Henkel Kgaa | Amylase und Protease enthaltende Wasch- und Reinigungsmittel |
| ATE547532T1 (de) | 1998-06-29 | 2012-03-15 | Bristol Myers Squibb Co | Verfahren zur erzeugung von hochgradig diversen bibliotheken |
| JP4335995B2 (ja) | 1998-07-22 | 2009-09-30 | 昭 神谷 | 環境保全型粒状洗浄用組成物 |
| FR2782323B1 (fr) | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
| US6277628B1 (en) | 1998-10-02 | 2001-08-21 | Incyte Genomics, Inc. | Linear microarrays |
| US6172248B1 (en) * | 1998-11-20 | 2001-01-09 | Ip Holdings, L.L.C. | Methods for refining vegetable oils and byproducts thereof |
| US6277489B1 (en) | 1998-12-04 | 2001-08-21 | The Regents Of The University Of California | Support for high performance affinity chromatography and other uses |
| US6511824B1 (en) | 1999-03-17 | 2003-01-28 | Exelixis, Inc. | Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use |
| US6309871B1 (en) | 1999-03-31 | 2001-10-30 | Novozymes A/S | Polypeptides having alkaline α-amylase activity |
| US6221653B1 (en) | 1999-04-27 | 2001-04-24 | Agilent Technologies, Inc. | Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids |
| US6653151B2 (en) | 1999-07-30 | 2003-11-25 | Large Scale Proteomics Corporation | Dry deposition of materials for microarrays using matrix displacement |
| EP1106603A3 (en) | 1999-12-06 | 2003-11-19 | Fuji Photo Film Co., Ltd. | DNA chip and reactive solid carrier |
| US6531644B1 (en) | 2000-01-14 | 2003-03-11 | Exelixis, Inc. | Methods for identifying anti-cancer drug targets |
| JP2004518413A (ja) | 2000-10-12 | 2004-06-24 | エクセリクシス・インコーポレイテッド | ヒトｅｃｔ２と使用方法 |
| US6309872B1 (en) | 2000-11-01 | 2001-10-30 | Novozymes Biotech, Inc | Polypeptides having glucoamylase activity and nucleic acids encoding same |
| US9409174B2 (en) | 2013-06-21 | 2016-08-09 | Bio-Rad Laboratories, Inc. | Microfluidic system with fluid pickups |
-
2003
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- 2003-04-21 CN CN038135949A patent/CN1659276A/zh active Pending
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- 2003-04-21 BR BR0309391-3A patent/BR0309391A/pt active IP Right Grant
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| EA010903B1 (ru) | 2008-12-30 |
| EA200401407A1 (ru) | 2006-02-24 |
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| AU2003243157A1 (en) | 2003-11-03 |
| BRPI0309391B1 (pt) | 2018-10-30 |
| CA2481411A1 (en) | 2003-10-30 |
| CN1659276A (zh) | 2005-08-24 |
| AU2003243157C1 (en) | 2008-09-04 |
| EP2298871A1 (en) | 2011-03-23 |
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