EA200900096A1 - Способ получения полипептидов - Google Patents

Способ получения полипептидов

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Publication number
EA200900096A1
EA200900096A1 EA200900096A EA200900096A EA200900096A1 EA 200900096 A1 EA200900096 A1 EA 200900096A1 EA 200900096 A EA200900096 A EA 200900096A EA 200900096 A EA200900096 A EA 200900096A EA 200900096 A1 EA200900096 A1 EA 200900096A1
Authority
EA
Eurasian Patent Office
Prior art keywords
suitability
methods
codon pairs
individual codons
host cells
Prior art date
Application number
EA200900096A
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English (en)
Other versions
EA015925B1 (ru
Inventor
Йоханнес Андрис Раубос
Ноэль Николас Мария Элизабет Пейдж Ван
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA200900096A1 publication Critical patent/EA200900096A1/ru
Publication of EA015925B1 publication Critical patent/EA015925B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение касается способов оптимизации кодирующих белки последовательностей для экспрессии в заданных клетках хозяина. В этих способах для оптимизации последовательностей, кодирующих заданную аминокислотную последовательность, по пригодности отдельных кодонов и/или по пригодности пар кодонов применяются генетические алгоритмы. При создании новых вариантов последовательностей и последующем отборе наилучших вариантов алгоритм выполняет итерацию до тех пор, пока варианты кодирующих последовательностей не достигнут минимального значения пригодности отдельных кодонов и/или пригодности пар кодонов. Изобретение также касается компьютера, включающего процессор и запоминающее устройство (память), причем компьютер настроен на чтение из памяти и запись в неё, при этом память включает данные и инструкции, предназначенные для придания процессору способности к выполнению генетических алгоритмов для оптимизации пригодности отдельных кодонов и/или пригодности пар кодонов. Изобретение также касается нуклеиновых кислот, содержащих кодирующую последовательность для заданной аминокислотной последовательности, причем кодирующая последовательность оптимизирована по пригодности отдельных кодонов и/или по пригодности пар кодонов для данного хозяина способами изобретения, а также клеток хозяина, содержащих такие нуклеиновые кислоты, и способов получения полипептидов и других продуктов ферментации, в которых применяются эти клетки хозяина.
EA200900096A 2006-06-29 2007-06-15 Способ получения полипептидов EA015925B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06076328 2006-06-29
PCT/EP2007/055943 WO2008000632A1 (en) 2006-06-29 2007-06-15 A method for achieving improved polypeptide expression

Publications (2)

Publication Number Publication Date
EA200900096A1 true EA200900096A1 (ru) 2009-06-30
EA015925B1 EA015925B1 (ru) 2011-12-30

Family

ID=37199000

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200900096A EA015925B1 (ru) 2006-06-29 2007-06-15 Способ получения полипептидов

Country Status (12)

Country Link
US (2) US8812247B2 (ru)
EP (2) EP2035561A1 (ru)
JP (1) JP5250850B2 (ru)
CN (1) CN101490262B (ru)
AU (1) AU2007263880A1 (ru)
BR (1) BRPI0713795B1 (ru)
CA (1) CA2657975A1 (ru)
DK (1) DK2423315T3 (ru)
EA (1) EA015925B1 (ru)
ES (1) ES2534282T3 (ru)
PL (1) PL2423315T3 (ru)
WO (1) WO2008000632A1 (ru)

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