DK3242950T3 - Crispr-cas-system til en trådformet svampeværtscelle - Google Patents

Crispr-cas-system til en trådformet svampeværtscelle Download PDF

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Publication number
DK3242950T3
DK3242950T3 DK16716466.4T DK16716466T DK3242950T3 DK 3242950 T3 DK3242950 T3 DK 3242950T3 DK 16716466 T DK16716466 T DK 16716466T DK 3242950 T3 DK3242950 T3 DK 3242950T3
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Denmark
Prior art keywords
mushroom
crispr
wired
host cell
cas system
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DK16716466.4T
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English (en)
Inventor
Bernard Meijrink
René Verwaal
Bianca Elisabeth Maria Gielesen
Johannes Andries Roubos
Roelof Arij Lans Bovenberg
Arnold Jacob Mathieu Driessen
Carsten Pohl
Yvonne Irene Nygård
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Dsm Ip Assets Bv
Univ Groningen
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
DK16716466.4T 2015-01-06 2016-01-06 Crispr-cas-system til en trådformet svampeværtscelle DK3242950T3 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP15150148 2015-01-06
EP15150164 2015-01-06
US201562177497P 2015-03-16 2015-03-16
US201562177495P 2015-03-16 2015-03-16
US201562177496P 2015-03-16 2015-03-16
PCT/EP2016/000029 WO2016110453A1 (en) 2015-01-06 2016-01-06 A crispr-cas system for a filamentous fungal host cell

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DK3242950T3 true DK3242950T3 (da) 2021-12-20

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US (1) US11396665B2 (da)
EP (1) EP3242950B1 (da)
DK (1) DK3242950T3 (da)
WO (1) WO2016110453A1 (da)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
WO2016100272A1 (en) * 2014-12-16 2016-06-23 Danisco Us Inc Fungal genome modification systems and methods of use
WO2017019867A1 (en) * 2015-07-28 2017-02-02 Danisco Us Inc Genome editing systems and methods of use
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
EP3412765B1 (en) * 2016-02-04 2023-01-18 Kao Corporation Method for producing mutant filamentous fungi
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
US11149268B2 (en) 2016-07-28 2021-10-19 Dsm Ip Assets B.V. Assembly system for a eukaryotic cell
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3516056A1 (en) * 2016-09-23 2019-07-31 DSM IP Assets B.V. A guide-rna expression system for a host cell
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US20200131487A1 (en) * 2017-03-06 2020-04-30 Institute For Basic Science Composition containing c2cl endonuclease for dielectric calibration and method for dielectric calibration using same
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
CN110431224A (zh) 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 锌双核簇转录调控因子缺陷型菌株
KR20190130613A (ko) 2017-03-23 2019-11-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018127611A1 (en) 2017-04-06 2018-07-12 Dsm Ip Assets B.V. Self-guiding integration construct (sgic)
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN110719956A (zh) 2017-06-06 2020-01-21 齐默尔根公司 用于改良真菌菌株的高通量基因组工程改造平台
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US20190002874A1 (en) 2017-06-30 2019-01-03 Inscripta, Inc. Cell libraries created using rationally designed nucleic acids
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019079347A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. USES OF BASIC EDITORS ADENOSINE
CN107858346B (zh) * 2017-11-06 2020-06-16 天津大学 一种敲除酿酒酵母染色体的方法
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
WO2019215102A1 (en) 2018-05-09 2019-11-14 Dsm Ip Assets B.V. Crispr transient expression construct (ctec)
BR112020024839A2 (pt) * 2018-06-06 2021-05-18 Zymergen Inc. manipulação de genes envolvidos em transdução de sinal para controlar a morfologia fúngica durante a fermentação e produção
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
US10604746B1 (en) 2018-10-22 2020-03-31 Inscripta, Inc. Engineered enzymes
EP3891281A1 (en) * 2018-12-05 2021-10-13 DSM IP Assets B.V. Crispr guide-rna expression strategies for multiplex genome engineering
KR20210143230A (ko) 2019-03-19 2021-11-26 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
US10837021B1 (en) 2019-06-06 2020-11-17 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
US10920189B2 (en) 2019-06-21 2021-02-16 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US20220389458A1 (en) 2019-11-04 2022-12-08 Dsm Ip Assets B.V. Low volume transfection
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
CN114787347A (zh) 2019-12-10 2022-07-22 因思科瑞普特公司 新颖的mad核酸酶
JP2023507566A (ja) 2019-12-18 2023-02-24 インスクリプタ， インコーポレイテッド 核酸誘導ヌクレアーゼ編集済み細胞のｉｎ ｖｉｖｏ検出のためのカスケード／ｄＣａｓ３相補性アッセイ
CA3157061A1 (en) 2020-01-27 2021-08-05 Christian SILTANEN Electroporation modules and instrumentation
CN111349649B (zh) * 2020-03-16 2020-11-17 三峡大学 一种用于双孢蘑菇的基因编辑的方法及应用
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
CN116096873A (zh) 2020-05-08 2023-05-09 布罗德研究所股份有限公司 同时编辑靶标双链核苷酸序列的两条链的方法和组合物
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
CN111979258B (zh) * 2020-08-04 2022-04-19 华中农业大学 一种高通量的基因编辑方法
US11299731B1 (en) 2020-09-15 2022-04-12 Inscripta, Inc. CRISPR editing to embed nucleic acid landing pads into genomes of live cells
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
EP4019627A1 (en) 2020-12-28 2022-06-29 Koninklijke Nederlandse Akademie van Wetenschappen New enzymes and methods for benzoic acid-related metabolic pathways
EP4271802A1 (en) 2021-01-04 2023-11-08 Inscripta, Inc. Mad nucleases
EP4274890A1 (en) 2021-01-07 2023-11-15 Inscripta, Inc. Mad nucleases
EP4032900A1 (en) 2021-01-21 2022-07-27 Koninklijke Nederlandse Akademie van Wetenschappen New enzymes and methods for vanillic acid-related metabolic pathways
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
WO2023023285A2 (en) * 2021-08-19 2023-02-23 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
CN114410635B (zh) * 2022-03-29 2022-06-14 中国科学院天津工业生物技术研究所 威尼斯镰刀菌内源U6启动子及其基于CRISPR/Cas9的基因编辑方法

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1341226C (en) 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
EP1321523A3 (en) 1993-07-23 2004-03-03 DSM IP Assets B.V. Selection marker gene free recombinant strains; a method for obtaining them and the use of these strains
DE69534185T2 (de) 1994-06-30 2006-02-23 Novozymes Biotech, Inc., Davis Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
DE69637784D1 (de) 1995-08-03 2009-02-05 Dsm Ip Assets Bv amdS-Gen aus Aspergillus niger für eine Acetamidase kodierend
EP2298913A1 (en) 1997-04-11 2011-03-23 DSM IP Assets B.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
DE69838106T2 (de) 1997-12-22 2008-04-03 Dsm Ip Assets B.V. Expressionsklonierung in filamentösen pilzen
ATE349528T1 (de) 1998-10-05 2007-01-15 Novozymes As Transkriptionsaktivator prtt aus dem pilz aspergillus niger verwendbar in verfahren zur herstellung von polypeptiden
CN1195058C (zh) 1999-02-22 2005-03-30 诺维信公司 草酰乙酸水解酶缺陷型真菌宿主细胞
AU2001242299A1 (en) 2000-03-14 2001-09-24 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
WO2004070022A2 (en) 2003-02-05 2004-08-19 Dsm Ip Assets B.V. Use of oxalate deficient aspergillus niger strains for producing a polypeptide
ES2294958B1 (es) 2003-07-15 2009-02-16 Mintek Procedimiento de lixiviacion oxidativa.
AU2004272629A1 (en) 2003-09-12 2005-03-24 Commonwealth Scientific And Industrial Research Organisation Modified gene-silencing nucleic acid molecules and uses thereof
DE602005023427D1 (de) 2004-04-02 2010-10-21 Dsm Ip Assets Bv Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz
DK1756145T3 (da) 2004-06-16 2014-09-29 Dsm Ip Assets Bv Produktion af polypeptider ved hjælp af forbedret sekretion
EP1799827A2 (en) 2004-10-12 2007-06-27 DSMIP Assets B.V. Fungal transcriptional activators useful in methods for producing a polypeptide
EP1799829B1 (en) 2004-10-15 2012-01-18 DSM IP Assets B.V. Method for production of a compound in a eukaryotic cell
JP2008516594A (ja) 2004-10-15 2008-05-22 ディーエスエム アイピー アセッツ ビー．ブイ． 選択可能なマーカーとしての相同性ａｍｄｓ遺伝子
AU2006207463B2 (en) 2005-01-24 2011-03-17 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
DK1954812T3 (da) 2005-11-29 2013-02-18 Dsm Ip Assets Bv DNA-bindingssted for en transskriptionsaktivator, der er anvendelig ved genekspression
EP2035561A1 (en) 2006-06-29 2009-03-18 DSMIP Assets B.V. A method for achieving improved polypeptide expression
WO2008053019A2 (en) 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Method for reducing the expression of a gene in a filamentous fungal cell
US9862956B2 (en) * 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2406386B1 (en) 2009-03-10 2019-01-23 DSM IP Assets B.V. Method for improving the yield of a polypeptide
AU2010241099A1 (en) 2009-04-22 2011-10-27 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
EP2449087A1 (en) 2009-07-03 2012-05-09 DSM IP Assets B.V. Talaromyces strains and enzyme compositions
CN102597241B (zh) 2009-07-22 2015-09-02 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的化合物的经改进的宿主细胞
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
EP2955221A1 (en) 2009-11-04 2015-12-16 DSM IP Assets B.V. Talaromyces transformants
EA201300074A1 (ru) 2010-07-01 2013-06-28 ДСМ АйПи АССЕТС Б.В. Способ получения представляющего интерес соединения
CN103596970A (zh) 2011-03-11 2014-02-19 帝斯曼知识产权资产管理有限公司 载体-宿主系统
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
CN104603273B (zh) 2012-03-12 2019-09-10 帝斯曼知识产权资产管理有限公司 重组系统
WO2013135732A1 (en) 2012-03-12 2013-09-19 Dsm Ip Assets B.V. Rasamsonia transformants
CN104981542B (zh) 2012-03-12 2018-10-16 帝斯曼知识产权资产管理有限公司 重组系统
DK2831238T3 (da) 2012-03-27 2018-04-03 Dsm Ip Assets Bv Kloningsfremgangsmåde
JP6343605B2 (ja) * 2012-05-25 2018-06-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ依存性標的ｄｎａ修飾およびｒｎａ依存性転写調節のための方法および組成物
DK2875119T3 (da) 2012-07-19 2018-04-03 Dsm Ip Assets Bv Agse-deficient stamme
EP2875120B1 (en) 2012-07-19 2019-03-20 DSM IP Assets B.V. Amylase-deficient strain
AU2014273085B2 (en) * 2013-05-29 2020-10-22 Cellectis New compact scaffold of Cas9 in the type II CRISPR system
KR20160035587A (ko) * 2013-07-10 2016-03-31 노파르티스 아게 복수개의 프로테아제 결핍 사상형 진균 세포들 및 그의 이용방법
WO2015017866A1 (en) * 2013-08-02 2015-02-05 Enevolv, Inc. Processes and host cells for genome, pathway, and biomolecular engineering
WO2015054507A1 (en) * 2013-10-10 2015-04-16 Pronutria, Inc. Nutritive polypeptide production systems, and methods of manufacture and use thereof
CN108064287A (zh) * 2015-01-06 2018-05-22 帝斯曼知识产权资产管理有限公司 用于解脂酵母宿主细胞的crispr-cas系统
WO2016110512A1 (en) * 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a yeast host cell

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EP3242950A1 (en) 2017-11-15
WO2016110453A1 (en) 2016-07-14
US20190194692A1 (en) 2019-06-27
US11396665B2 (en) 2022-07-26
WO2016110453A8 (en) 2016-12-22

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