DE602005023427D1 - Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz - Google Patents

Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz

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Publication number
DE602005023427D1
DE602005023427D1 DE602005023427T DE602005023427T DE602005023427D1 DE 602005023427 D1 DE602005023427 D1 DE 602005023427D1 DE 602005023427 T DE602005023427 T DE 602005023427T DE 602005023427 T DE602005023427 T DE 602005023427T DE 602005023427 D1 DE602005023427 D1 DE 602005023427D1
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efficiency
nhr
mutanes
improved
pathway
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Inventor
Petrus Jacobus Dekker
Van Den Marco Alexan Berg
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DSM IP Assets BV
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DSM IP Assets BV
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/385—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Penicillium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/80—Penicillium
    • C12R2001/82—Penicillium chrysogenum

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Mushroom Cultivation (AREA)
DE602005023427T 2004-04-02 2005-03-31 Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz Active DE602005023427D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04076057 2004-04-02
PCT/EP2005/051464 WO2005095624A2 (en) 2004-04-02 2005-03-31 Filamentous fungal mutants with improved homologous recombination efficiency

Publications (1)

Publication Number Publication Date
DE602005023427D1 true DE602005023427D1 (de) 2010-10-21

Family

ID=34966505

Family Applications (1)

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DE602005023427T Active DE602005023427D1 (de) 2004-04-02 2005-03-31 Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz

Country Status (12)

Country Link
US (2) US9243043B2 (de)
EP (3) EP1733040B1 (de)
JP (2) JP2007530065A (de)
CN (1) CN101218348B (de)
AT (1) ATE480632T1 (de)
AU (1) AU2005229437A1 (de)
CA (1) CA2559827A1 (de)
DE (1) DE602005023427D1 (de)
DK (1) DK1733040T3 (de)
ES (1) ES2350048T3 (de)
PL (1) PL1733040T3 (de)
WO (1) WO2005095624A2 (de)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE510925T1 (de) 2003-06-25 2011-06-15 Novozymes As Stärkehydrolyseverfahren
EP1733040B1 (de) 2004-04-02 2010-09-08 DSM IP Assets B.V. Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz
JP4625318B2 (ja) * 2004-12-06 2011-02-02 財団法人野田産業科学研究所 相同組換え頻度が上昇した形質転換菌
DK2410048T3 (en) 2005-01-24 2016-11-28 Dsm Ip Assets Bv A process for the preparation of a compound of interest in a filamentous fungal cell
AU2006219925A1 (en) * 2005-03-01 2006-09-08 Dsm Ip Assets B.V. Aspergillus promotors for expressing a gene in a fungal cell
CN101454455A (zh) 2005-11-29 2009-06-10 帝斯曼知识产权资产管理有限公司 在基因表达中有用的转录激活因子的dna结合位点
BRPI0619091A2 (pt) 2005-12-01 2011-09-13 Dsm Ip Assests Bv novos genes úteis para a produção industrial de ácido cìtrico
EP2035561A1 (de) 2006-06-29 2009-03-18 DSMIP Assets B.V. Verfahren für verbesserte polypeptidexpression
EP2511372A1 (de) 2006-11-02 2012-10-17 DSM IP Assets B.V. Verbesserte Herstellung von sekretierten Proteinen durch filamentöse Pilze
AU2008214663B2 (en) 2007-02-15 2013-10-03 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
US20100120154A1 (en) 2007-03-21 2010-05-13 Dsm Ip Assets B.V. Method for homologous recombination
WO2008138835A1 (en) 2007-05-09 2008-11-20 Novozymes A/S Expression cloning method suitable for selecting library clones producing a polypeptide of interest
EP2123772A1 (de) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-Lactamantibiotikum produzierende Stämme
EP2392649A3 (de) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA-produzierende Stämme
CA2754387C (en) 2009-03-10 2018-11-13 Dsm Ip Assets B.V. Pregastric esterase and derivatives thereof
JP2012520064A (ja) 2009-03-10 2012-09-06 ディーエスエム アイピー アセッツ ビー．ブイ． ポリペプチドの収量を改良する方法
EP2406385A2 (de) 2009-03-11 2012-01-18 DSM IP Assets B.V. Herstellung von adipinsäure
EP2421986A1 (de) 2009-04-22 2012-02-29 DSM IP Assets B.V. Verfahren zur herstellung eines interessierenden rekombinanten polypeptids
DK2421965T3 (en) 2009-04-24 2016-09-05 Dsm Ip Assets Bv Carbohydratdegraderende polypeptide and uses thereof
CN102597241B (zh) 2009-07-22 2015-09-02 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的化合物的经改进的宿主细胞
AU2010317063B2 (en) 2009-11-04 2014-10-23 Dsm Ip Assets B.V. Talaromyces transformants
AU2011212619A1 (en) 2010-02-04 2012-08-09 Dsm Ip Assets B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
US9109214B2 (en) 2010-02-11 2015-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
AU2011214327B2 (en) 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
ES2672125T3 (es) 2010-06-29 2018-06-12 Dsm Ip Assets B.V. Polipéptido que tiene actividad beta-glucosidasa y usos del mismo
ES2628345T3 (es) 2010-06-29 2017-08-02 Dsm Ip Assets B.V. Polipéptido que tiene o que ayuda en la actividad de degradación de materiales glucídicos y usos del mismo
EA201300058A1 (ru) 2010-06-29 2013-06-28 ДСМ АйПи АССЕТС Б.В. Полипептид, обладающий бета-глюкозидазной активностью, и его применение
BR112012033396A2 (pt) 2010-06-29 2015-06-23 Dsm Ip Assets Bv Polipetídeo tendo atividade swollenin e seus usos.
US9109213B2 (en) 2010-06-29 2015-08-18 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
CN103391999B (zh) 2010-06-29 2016-06-22 帝斯曼知识产权资产管理有限公司 具有乙酰基木聚糖酯酶活性的多肽及其用途
EA201300074A1 (ru) 2010-07-01 2013-06-28 ДСМ АйПи АССЕТС Б.В. Способ получения представляющего интерес соединения
WO2012031910A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Method for preparing alpha-ketopimelic acid by c1-elongation
DK2683732T3 (en) 2011-03-11 2016-12-12 Dsm Ip Assets Bv Vector-host-system
WO2013076280A1 (en) 2011-11-23 2013-05-30 Dsm Ip Assets B.V. Nucleic acid assembly system
BR112014031526A2 (pt) 2012-06-19 2017-08-01 Dsm Ip Assets Bv promotores para expressar um gene em uma célula
DK3327112T3 (en) 2012-07-19 2020-05-04 Dsm Ip Assets Bv Agse-deficient stamme
RU2015115890A (ru) * 2012-09-28 2016-11-20 Юсб Биофарма Спрл Способы получения рекомбинантных белков
MY176331A (en) 2013-02-04 2020-07-29 Dsm Ip Assets Bv Carbohydrate degrading polypeptide and uses thereof
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
ES2706742T3 (es) 2013-12-02 2019-04-01 Dsm Ip Assets Bv Proteína estructuradora del hielo
WO2015086798A2 (en) * 2013-12-13 2015-06-18 Cellectis New method of selection of algal-transformed cells using nuclease
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
WO2016110512A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a yeast host cell
EP3302076A1 (de) 2015-06-02 2018-04-11 DSM IP Assets B.V. Verwendung von in filamentösen pilzstämmen exprimiertem eisstrukturprotein afp19 zur herstellung von lebensmitteln
EP3387134B1 (de) * 2015-12-11 2020-10-14 Danisco US Inc. Verfahren und zusammensetzungen für verbesserte nukleasevermittelte genommodifizierung und verringerte off-target-nebenwirkungen
US10913938B2 (en) 2016-07-29 2021-02-09 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
KR102593668B1 (ko) * 2016-10-04 2023-10-24 다니스코 유에스 인크. 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산
EP3559221A1 (de) 2016-12-21 2019-10-30 DSM IP Assets B.V. Varianten lipolytischer enzyme
WO2018114941A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
EP3635112A2 (de) 2017-06-06 2020-04-15 Zymergen, Inc. Genomische htp-engineering-plattform zur verbesserung von pilzstämmen
JP2021526799A (ja) 2018-06-06 2021-10-11 ザイマージェン インコーポレイテッド 発酵および産生中の真菌の形態を制御するためのシグナル伝達に関与する遺伝子の操作
WO2021089452A1 (en) 2019-11-04 2021-05-14 Dsm Ip Assets B.V. Low volume transfection
CN111088173B (zh) * 2019-12-26 2021-08-20 南京高新工大生物技术研究院有限公司 一株黑曲霉基因工程菌及其构建方法和应用
WO2021139779A1 (zh) * 2020-01-09 2021-07-15 中国科学院分子植物科学卓越创新中心 一种优化的真菌双体外基因组编辑方法
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
EP4019627A1 (de) 2020-12-28 2022-06-29 Koninklijke Nederlandse Akademie van Wetenschappen Neue enzyme und verfahren für benzoesäurebezogene stoffwechselwege
EP4032900A1 (de) 2021-01-21 2022-07-27 Koninklijke Nederlandse Akademie van Wetenschappen Neue enzyme und verfahren für vanillesäurebezogene stoffwechselwege
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8416212D0 (en) 1984-06-26 1984-08-01 Sturge John & E Ltd Lactase containing compositions
CA1341226C (en) 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
DE69432543T2 (de) 1993-07-23 2003-12-24 Dsm Nv Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
EP0758020B1 (de) 1995-08-03 2008-12-24 DSM IP Assets B.V. amdS-Gen aus Aspergillus niger für eine Acetamidase kodierend
US6432672B1 (en) 1997-04-11 2002-08-13 Gerardus Cornelis Maria Selten Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
WO1999053017A2 (en) 1998-04-15 1999-10-21 Fred Hutchinson Cancer Research Center Methods and vector constructs for making transgenic non-human animals which ubiquitously express a heterologous gene
GB9907687D0 (en) 1999-04-01 1999-05-26 Kudos Pharm Ltd Assays methods and means
AU1647501A (en) 1999-12-10 2001-06-18 Cytos Biotechnology Ag Replicon based activation of endogenous genes
US6569681B1 (en) * 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
DE60132801T2 (de) 2000-12-07 2009-02-12 Dsm Ip Assets B.V. Prolylendoprotease aus aspergillus niger
EP1217074A1 (de) 2000-12-22 2002-06-26 Universiteit Leiden Integration von Nukleinsäure in Eukaryoten
US20080108056A1 (en) * 2001-03-30 2008-05-08 Masayuki Machida Method of Detecting the Expression of Aspergillus Gene
DE10131786A1 (de) 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20030092183A1 (en) 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
AU2003231028A1 (en) 2002-04-22 2003-11-03 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
US20070298455A1 (en) 2003-10-14 2007-12-27 Van Den Berg Marco A Method For Preparing A Modified Host Cell
CA2548220A1 (en) 2003-12-01 2005-09-09 Introgen Therapeutics, Inc. Use of mda-7 to inhibit infection by pathogenic organisms
US20050181509A1 (en) 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
JP4050712B2 (ja) * 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 相同組換えを行わせる方法
EP1733040B1 (de) 2004-04-02 2010-09-08 DSM IP Assets B.V. Filamentöse pilzmutanten mit verbesserter homologer rekombinationseffizienz
JP2006058093A (ja) * 2004-08-18 2006-03-02 National Institute For Materials Science 血液分析装置
EP1794305A2 (de) 2004-09-23 2007-06-13 BASF Plant Science GmbH Rekombinationskassetten und verfahren zur sequenz-exzision in pflanzen
US20070155014A1 (en) 2005-07-20 2007-07-05 Invitrogen Corporation Methods for increasing efficiency of homologous recombination
WO2007115886A1 (en) 2006-04-08 2007-10-18 Dsm Ip Assets B.V. Improved method for homologous recombination in eukaryotic cells
US20100120154A1 (en) 2007-03-21 2010-05-13 Dsm Ip Assets B.V. Method for homologous recombination
US8597923B2 (en) 2009-05-06 2013-12-03 SyntheZyme, LLC Oxidation of compounds using genetically modified Candida

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Publication number Publication date
EP1733040A2 (de) 2006-12-20
CA2559827A1 (en) 2005-10-13
EP1733040B1 (de) 2010-09-08
US9243043B2 (en) 2016-01-26
US9657301B2 (en) 2017-05-23
DK1733040T3 (da) 2010-11-22
EP2172557A1 (de) 2010-04-07
EP3351637B1 (de) 2020-06-10
WO2005095624A3 (en) 2006-02-16
PL1733040T3 (pl) 2011-01-31
EP2172557B1 (de) 2018-03-14
US20160130589A1 (en) 2016-05-12
ATE480632T1 (de) 2010-09-15
AU2005229437A1 (en) 2005-10-13
CN101218348A (zh) 2008-07-09
JP2007530065A (ja) 2007-11-01
CN101218348B (zh) 2014-07-30
WO2005095624A2 (en) 2005-10-13
US20080227145A1 (en) 2008-09-18
EP2172557A8 (de) 2010-06-23
ES2350048T3 (es) 2011-01-17
JP2011152148A (ja) 2011-08-11
EP3351637A1 (de) 2018-07-25

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