DE69432709T2 - Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen - Google Patents

Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen

Info

Publication number
DE69432709T2
DE69432709T2 DE69432709T DE69432709T DE69432709T2 DE 69432709 T2 DE69432709 T2 DE 69432709T2 DE 69432709 T DE69432709 T DE 69432709T DE 69432709 T DE69432709 T DE 69432709T DE 69432709 T2 DE69432709 T2 DE 69432709T2
Authority
DE
Germany
Prior art keywords
nucleic acid
capture
label
bound
hybridizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69432709T
Other languages
German (de)
English (en)
Other versions
DE69432709D1 (de
Inventor
Mark Collins
Timothy Fultz
S. Urdea
D. Warner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Publication of DE69432709D1 publication Critical patent/DE69432709D1/de
Application granted granted Critical
Publication of DE69432709T2 publication Critical patent/DE69432709T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
    • F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
    • F02B75/00—Other engines
    • F02B75/02—Engines characterised by their cycles, e.g. six-stroke
    • F02B2075/022—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle
    • F02B2075/027—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle four
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE69432709T 1993-12-08 1994-12-07 Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen Expired - Lifetime DE69432709T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/164,388 US5681697A (en) 1993-12-08 1993-12-08 Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
PCT/US1994/014119 WO1995016055A1 (en) 1993-12-08 1994-12-07 Solution phase nucleic acid sandwich assays having reduced background noise

Publications (2)

Publication Number Publication Date
DE69432709D1 DE69432709D1 (de) 2003-06-26
DE69432709T2 true DE69432709T2 (de) 2003-11-20

Family

ID=22594262

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69432709T Expired - Lifetime DE69432709T2 (de) 1993-12-08 1994-12-07 Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen

Country Status (11)

Country Link
US (2) US5681697A (enExample)
EP (1) EP0731848B1 (enExample)
JP (4) JP3802925B2 (enExample)
KR (1) KR100327610B1 (enExample)
AT (1) ATE241017T1 (enExample)
AU (1) AU694468B2 (enExample)
CA (1) CA2178598A1 (enExample)
DE (1) DE69432709T2 (enExample)
ES (1) ES2199236T3 (enExample)
HU (1) HU221237B1 (enExample)
WO (1) WO1995016055A1 (enExample)

Families Citing this family (352)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7825215B1 (en) * 1993-04-26 2010-11-02 Peter E. Nielsen Substituted nucleic acid mimics
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
DE69528670T2 (de) * 1994-12-23 2004-03-11 Dade Behring Inc., Deerfield Nachweis von nukleinsäuren durch nuklease-katalysierte produktbildung
FR2737502B1 (fr) 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
WO1997023646A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
CA2239683A1 (en) * 1995-12-22 1997-07-03 Dade Behring Marburg Gmbh Homogeneous amplification and detection of nucleic acids
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
US5902724A (en) 1996-07-12 1999-05-11 Tm Technologies, Inc. Signal amplification method
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
DK0975807T3 (da) * 1997-05-02 2007-01-29 Gen Probe Inc To-trins hybridisering og indfangning af et polynukleotid
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US5939261A (en) * 1997-06-24 1999-08-17 Sarnoff Corporation Method for capturing a nucleic acid
CA2313641A1 (en) * 1997-12-12 1999-06-24 Digene Corporation Universal collection medium
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
WO1999036574A1 (en) * 1998-01-20 1999-07-22 Mayo Foundation For Medical Education And Research Simplified mutation detection
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
GB9805935D0 (en) * 1998-03-19 1998-05-13 Ciba Geigy Ag Organic compounds
WO2000004192A1 (en) * 1998-07-17 2000-01-27 Emory University Methods for detecting and mapping genes, mutations and variant polynucleotide sequences
ATE234468T1 (de) 1998-09-18 2003-03-15 Massachusetts Inst Technology Biologische verwendungen von halbleitenden nanokristallen
US6203989B1 (en) * 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
US20030032029A1 (en) * 1998-12-21 2003-02-13 Nanogen, Inc. Three dimensional apparatus and method for integrating sample preparation and multiplex assays
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
WO2000050640A1 (en) 1999-02-25 2000-08-31 Exact Laboratories, Inc. Methods for preserving dna integrity
GB9905580D0 (en) * 1999-03-12 1999-05-05 Tepnel Medical Ltd Enyzymatically catalysed signal amplification
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
DE60040741D1 (de) 1999-04-20 2008-12-18 Illumina Inc Detektion von nukleinsäurereaktionen auf kügelchen-arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20020045182A1 (en) * 1999-07-16 2002-04-18 Lynx Therapeutics, Inc. Multiplexed differential displacement for nucleic acid determinations
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
ATE458831T1 (de) * 1999-12-07 2010-03-15 Exact Sciences Corp Verfahren zum nachweis von lungenneoplasmen in fäkalen proben
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
JP2003521252A (ja) 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US20020094538A1 (en) * 2001-01-13 2002-07-18 Getts Robert C. Methods for detecting and assaying nucleic acid sequences using temperature cycling
WO2001073122A1 (en) * 2000-03-28 2001-10-04 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US20030211496A1 (en) * 2000-03-29 2003-11-13 Getts Robert C Quality control reagents for nucleic acid microarrays
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
GB0016813D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
GB0016833D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
GB0016814D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (3)
CA2731495C (en) 2000-09-01 2015-02-03 Gen-Probe Incorporated Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
US20030044803A1 (en) * 2000-09-22 2003-03-06 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of JAK1
US20020115058A1 (en) * 2000-09-22 2002-08-22 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of Pik3r1
US20030077590A1 (en) * 2000-09-22 2003-04-24 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of neurogranin
US6806051B2 (en) * 2000-09-25 2004-10-19 Picoliter Inc. Arrays of partially nonhybridizing oligonucleotides and preparation thereof using focused acoustic energy
WO2002031206A2 (en) * 2000-10-11 2002-04-18 Ragland William L Methods and compositions using hybridization assays for detecting infectious agents
US7892730B2 (en) 2000-12-22 2011-02-22 Sagres Discovery, Inc. Compositions and methods for cancer
US7700274B2 (en) * 2000-12-22 2010-04-20 Sagres Discovery, Inc. Compositions and methods in cancer associated with altered expression of KCNJ9
US20030232334A1 (en) 2000-12-22 2003-12-18 Morris David W. Novel compositions and methods for cancer
US7645441B2 (en) 2000-12-22 2010-01-12 Sagres Discovery Inc. Compositions and methods in cancer associated with altered expression of PRLR
US20030099963A1 (en) * 2000-12-22 2003-05-29 Morris David W. Novel compositions and methods in cancer associated with altered expression of TBX21
US20030165878A1 (en) * 2000-12-22 2003-09-04 Morris David W. Novel compositions and methods in cancer associated with altered expression of MCM3AP
US7820447B2 (en) 2000-12-22 2010-10-26 Sagres Discovery Inc. Compositions and methods for cancer
US20030087252A1 (en) * 2000-12-22 2003-05-08 Morris David W. Novel compositions and methods in cancer associated with altered expression of PRDM11
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US20030092157A1 (en) * 2001-03-16 2003-05-15 Hayden Michael R. Compositions, screening systems and methods for modulating HDL cholesterol and triglyceride levels
US20030082571A1 (en) * 2001-04-10 2003-05-01 Kachab Edward Hanna Linear nucleic acid and sequence therefor
US20030191073A1 (en) 2001-11-07 2003-10-09 Challita-Eid Pia M. Nucleic acid and corresponding protein entitled 161P2F10B useful in treatment and detection of cancer
AU2002305019A1 (en) * 2001-05-25 2002-12-09 University Of British Columbia Diagnostic methods for cardiovascular disease, low hdl-cholesterol levels, and high triglyceride levels
US7291457B2 (en) * 2001-06-21 2007-11-06 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
US20070098728A1 (en) * 2001-09-24 2007-05-03 Pedersen Finn S Novel compositions and methods in cancer
US20040166490A1 (en) * 2002-12-17 2004-08-26 Morris David W. Novel therapeutic targets in cancer
US20040126762A1 (en) * 2002-12-17 2004-07-01 Morris David W. Novel compositions and methods in cancer
US20060040262A1 (en) * 2002-12-27 2006-02-23 Morris David W Novel compositions and methods in cancer
US20040197778A1 (en) * 2002-12-26 2004-10-07 Sagres Discovery, Inc. Novel compositions and methods in cancer
US20040180344A1 (en) * 2003-03-14 2004-09-16 Morris David W. Novel therapeutic targets in cancer
US6635428B2 (en) * 2001-12-04 2003-10-21 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis B viral nucleic acids
US6946245B2 (en) * 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
EP1463829A1 (en) * 2001-12-19 2004-10-06 Quantibact A/S A method and a kit for determination of a microbial count
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
US7776524B2 (en) * 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
WO2003080808A2 (en) * 2002-03-21 2003-10-02 Sagres Discovery, Inc. Novel compositions and methods in cancer
CA2480673A1 (en) * 2002-03-29 2003-10-09 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
AU2003243562A1 (en) * 2002-06-12 2003-12-31 Datascope Investment Corp. Polymeric label molecules
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
EP1389638A1 (en) * 2002-08-06 2004-02-18 Roche Diagnostics GmbH Improved fluorescent resonance energy transfer probes
US20040081653A1 (en) 2002-08-16 2004-04-29 Raitano Arthur B. Nucleic acids and corresponding proteins entitled 251P5G2 useful in treatment and detection of cancer
EP1563089A4 (en) * 2002-08-30 2007-09-19 Bayer Healthcare Llc SOLID PHASE-BASED NUCLEIC ACID ASSAYS WITH HIGH AFFINITY AND SPECIFICITY
EP1561109A1 (de) * 2002-09-03 2005-08-10 Zeptosens AG Analytische plattform und nachweisverfahren mit den in einer probe nachzuweisenden analyten als immobilisierten spezifischen bindungspartnern
EP1567673A2 (en) * 2002-09-13 2005-08-31 Hvidovre Hospital Method of rapid detection of mutations and nucleotide polymorphisms using chemometrics
AU2003280692A1 (en) * 2002-10-31 2004-05-25 Mitsubishi Chemical Corporation Electrolyte for electrolytic capacitor, electrolytic capacitor and process for producing tetrafluoroaluminate salt of organic onium
EP1585832B1 (en) * 2002-12-20 2008-02-13 Stratagene Compositions and methods for polynucleotide detection
CA2860151A1 (en) 2003-02-10 2004-08-26 Agensys, Inc. Nucleic acid and corresponding protein named 158p1d7 useful in the treatment and detection of bladder and other cancers
US20070218071A1 (en) * 2003-09-15 2007-09-20 Morris David W Novel therapeutic targets in cancer
JP2007524362A (ja) 2003-02-14 2007-08-30 サイグレス ディスカバリー， インコーポレイテッド 癌における治療ｇｐｃｒ標的
US7767387B2 (en) * 2003-06-13 2010-08-03 Sagres Discovery, Inc. Therapeutic targets in cancer
US20040170982A1 (en) * 2003-02-14 2004-09-02 Morris David W. Novel therapeutic targets in cancer
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
US7229763B2 (en) * 2003-04-07 2007-06-12 Beckman Coulter, Inc. Assay system using labeled oligonucleotides
EA009302B1 (ru) * 2003-05-20 2007-12-28 Инвестиген, Инк. Система обнаружения полинуклеотидов
AU2004263823B2 (en) 2003-05-30 2008-09-18 Agensys, Inc. Prostate stem cell antigen (PSCA) variants and subsequences thereof
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
US20040259100A1 (en) 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
DE20310332U1 (de) * 2003-07-04 2004-11-11 Mwg-Biotech Ag Vorrichtung zum automatischen Öffnen und Schließen von Reaktionsgefäßen
US20060035242A1 (en) 2004-08-13 2006-02-16 Michelitsch Melissa D Prion-specific peptide reagents
WO2005040074A2 (en) * 2003-08-29 2005-05-06 Dendritic Nanotechnologies, Inc. Crick-watson base paired dendrimer structures
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20070281896A1 (en) * 2003-09-30 2007-12-06 Morris David W Novel compositions and methods in cancer
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2005111242A2 (en) * 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
US20080124714A1 (en) * 2004-05-14 2008-05-29 Exact Sciences Corporation Method for Stabilizing Biological Samples for Nucleic Acid Analysis
CA2566519C (en) 2004-05-14 2020-04-21 Rosetta Genomics Ltd. Micrornas and uses thereof
EP2322665A1 (en) 2004-05-14 2011-05-18 Rosetta Genomics Ltd MicroRNAs and uses thereof
US7473551B2 (en) 2004-05-21 2009-01-06 Atonomics A/S Nano-mechanic microsensors and methods for detecting target analytes
RU2381234C2 (ru) 2004-05-28 2010-02-10 Эдженсис, Инк. Антитела и родственные молекулы, связывающиеся с белками psca
JP5020818B2 (ja) 2004-07-01 2012-09-05 ジェン−プローブ・インコーポレーテッド 生物学的試料中の核酸を検出するための方法及び組成物
US20060024677A1 (en) 2004-07-20 2006-02-02 Morris David W Novel therapeutic targets in cancer
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US7482122B1 (en) * 2004-10-19 2009-01-27 Hai Xing Chen Method of signal enhancement for measurement of nucleic acid sequences
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
CN101137758B (zh) * 2004-11-03 2012-10-10 意力速分子诊断股份有限公司 均相分析物检测
WO2006076683A2 (en) * 2005-01-13 2006-07-20 Novartis Vaccines And Diagnostics Inc. Isolation and detection of pathogenic prions
EP1921454B1 (en) 2005-03-10 2015-08-12 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes
US7794659B2 (en) 2005-03-10 2010-09-14 Gen-Probe Incorporated Signal measuring system having a movable signal measuring device
EP3300739A3 (en) 2005-03-31 2018-07-18 Agensys, Inc. Antibodies and related molecules that bind to 161p2f10b proteins
CA2604844A1 (en) 2005-04-07 2006-10-19 Novartis Vaccines And Diagnostics, Inc. Cancer-related genes
AU2006235276A1 (en) 2005-04-07 2006-10-19 Novartis Vaccines And Diagnostics Inc. CACNA1E in cancer diagnosis, detection and treatment
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US7993853B2 (en) 2005-05-06 2011-08-09 Gen-Probe Incorporated Methods of nucleic acid target capture
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1880206B1 (en) 2005-05-09 2012-11-14 Affymetrix, Inc. Multiplex capture of nucleic acids
ATE502120T1 (de) * 2005-05-12 2011-04-15 Affymetrix Inc Multiplex-assays für verzweigtkettige dna
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
EP3042963A1 (en) 2005-06-20 2016-07-13 Advanced Cell Diagnostics, Inc. Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
KR20080047355A (ko) * 2005-07-06 2008-05-28 바이오칩 이노베이션즈 피티와이 리미티드 표적 핵산 서열 분석 방법 및 키트
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
US7410763B2 (en) * 2005-09-01 2008-08-12 Intel Corporation Multiplex data collection and analysis in bioanalyte detection
US20120231453A1 (en) * 2005-09-13 2012-09-13 Affymetrix, Inc. Brownian Microbarcodes for Bioassays
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
US8017360B2 (en) * 2005-11-10 2011-09-13 Panomics, Inc. Detection of nucleic acids through amplification of surrogate nucleic acids
WO2007087312A2 (en) * 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US8021839B2 (en) * 2006-02-24 2011-09-20 Investigen, Inc. Methods and compositions for detecting polynucleotides
WO2007127564A2 (en) 2006-04-26 2007-11-08 Siemens Medical Solutions Diagnostics Gmbh Solid phase based nucleic acid assays combining high affinity capturing and detection by specific hybridization
WO2008029295A2 (en) 2006-06-30 2008-03-13 Rosetta Genomics Ltd A method for detecting nucleic acids
JP2008043332A (ja) * 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
JP2008064475A (ja) * 2006-09-04 2008-03-21 Osaka Univ 標的物質の高感度検出方法、検出用キットおよび検出装置
WO2008052774A2 (en) * 2006-10-31 2008-05-08 Noxxon Pharma Ag Methods for detection of a single- or double-stranded nucleic acid molecule
US7615351B2 (en) 2006-12-01 2009-11-10 Panomics, Inc. Two stage nucleic acid amplification using an amplification oligomer
US8481506B2 (en) * 2006-12-05 2013-07-09 Rosetta Genomics, Ltd. Nucleic acids involved in viral infection
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20110136099A1 (en) * 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US7855054B2 (en) * 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20080241140A1 (en) 2007-02-12 2008-10-02 Medical College Of Georgia Gene amplification of coactivator coaa and uses thereof
US7771947B2 (en) * 2007-02-23 2010-08-10 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
JP5211790B2 (ja) * 2007-03-26 2013-06-12 住友化学株式会社 Ｄｎａメチル化測定方法
US7794985B2 (en) * 2007-04-04 2010-09-14 Ghc Technologies, Inc. Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins
WO2008122960A2 (en) * 2007-04-10 2008-10-16 Microvacuum Ltd. Label-free optical detection method
WO2009022682A1 (ja) * 2007-08-14 2009-02-19 Eisai R & D Management Co., Ltd. 標的物質の検出方法
EP3136103B1 (en) 2007-10-05 2018-08-29 Affymetrix, Inc. Highly multiplexed assays
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
JP2011520108A (ja) * 2008-04-30 2011-07-14 ノバルティス アーゲー 病原性配座異性体についてのアッセイ
US10316352B2 (en) * 2008-05-13 2019-06-11 Gen-Probe Incorporated Methods of capturing a target nucleic acid for amplification and detection using an inactivatable target capture oligomer
US20090298709A1 (en) * 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
US8703416B2 (en) 2008-07-17 2014-04-22 Somalogic, Inc. Method for purification and identification of sperm cells
US20100076185A1 (en) * 2008-09-22 2010-03-25 Nils Adey Selective Processing of Biological Material on a Microarray Substrate
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
TWI419974B (zh) * 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
US9150910B2 (en) 2008-11-17 2015-10-06 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using linking molecules
EP2358898B1 (en) 2008-11-17 2016-08-31 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using covalent bond forming reactive pairs
DK2189539T4 (en) * 2008-11-21 2018-09-17 Chimera Biotec Gmbh Conjugate complexes for analyte detection
JP2012509675A (ja) 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド 低分子ｒｎａを検出するための組成物および方法、ならびにその使用
CN102257160A (zh) 2008-12-18 2011-11-23 西门子医疗保健诊断公司 用于缩短杂交分析中的培养时间的方法和试剂
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
EP2399129B1 (en) 2009-02-20 2015-11-25 Michael P. Lisanti A method of diagnosis or prognosis of a neoplasm comprising determining the level of expression of a protein in stromal cells adjacent to the neoplasm
AU2010242867B2 (en) 2009-05-01 2016-05-12 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of RNA splice-forms in a sample
EP2478087B1 (en) 2009-09-14 2017-01-18 QIAGEN Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
CN102725639A (zh) 2009-11-04 2012-10-10 诺华有限公司 蛋白质聚集物与单体分离中作为结合试剂的带正电物质
WO2011071923A2 (en) 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011084168A1 (en) * 2010-01-08 2011-07-14 The Regents Of The University Of California Bioassays based on polymeric sequence probes
WO2011094514A1 (en) * 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
EP2529030B1 (en) 2010-01-29 2019-03-13 Advanced Cell Diagnostics, Inc. Methods of in situ detection of nucleic acids
DK2539355T3 (da) * 2010-02-26 2017-01-02 Ventana Med Syst Inc IN-SITU-hybridisering med polytag-prober
EP2572001A2 (en) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CA2799205A1 (en) 2010-05-25 2011-12-01 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
ES2562821T3 (es) * 2010-10-21 2016-03-08 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
US9255293B2 (en) 2010-11-01 2016-02-09 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
US20120178081A1 (en) * 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
CN103476951B (zh) 2011-02-16 2017-07-28 海德威技术公司 用于可检测的标记的靶标定位锚定的方法和组合物
EP2678442B1 (en) 2011-02-24 2019-01-16 QIAGEN Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
JP6430253B2 (ja) * 2011-11-05 2018-11-28 プレジデント アンド フェローズ オブ ハーバード カレッジ 相互作用を検出および測定するための核酸ベースのリンカー
BR112014012288A2 (pt) 2011-11-22 2017-05-23 Intermune Inc métodos para diagnosticar e tratar fibrose pulmonar idiopática
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
GB2523211B (en) 2012-01-27 2020-03-18 Univ Jefferson MCT protein inhibitor-related prognostic and therapeutic methods
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
EP3287531B1 (en) 2012-02-28 2019-06-19 Agilent Technologies, Inc. Method for attaching a counter sequence to a nucleic acid sample
EP2824180B1 (en) * 2012-03-05 2019-04-03 NGK Insulators, Ltd. Method for detecting target nucleic acid
US20140178869A1 (en) 2012-04-05 2014-06-26 Advanced Cell Diagnostics, Inc. Detection of immunoglobulin light chain restriction by rna in situ hybridization
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
PL2893040T3 (pl) 2012-09-04 2019-05-31 Guardant Health Inc Sposoby wykrywania rzadkich mutacji i wariantu liczby kopii
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
WO2014074785A1 (en) 2012-11-08 2014-05-15 Ludwig Institute For Cancer Research Ltd. Methods of predicting outcome and treating breast cancer
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US20160024565A1 (en) * 2013-03-13 2016-01-28 Tufts University Fragment complementation of based assays
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
EP2986290A4 (en) 2013-04-19 2017-01-04 Thomas Jefferson University Caveolin-1 related methods for treating glioblastoma with temozolomide
EP3434784A1 (en) * 2013-06-14 2019-01-30 Nanostring Technologies, Inc Multiplexable tag-based reporter system
US9745633B2 (en) * 2013-06-28 2017-08-29 Diacarta Ltd Detection of PNA clamping
DE102013011304A1 (de) * 2013-07-02 2015-01-22 Technische Universität Dresden Verfahren und Anordnung zur Erfassung von Bindungsereignissen von Molekülen
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
CN105745528A (zh) 2013-10-07 2016-07-06 赛卢拉研究公司 用于以数字方式对阵列上的特征进行计数的方法和系统
CN111534580B (zh) 2013-12-28 2024-06-04 夸登特健康公司 用于检测遗传变异的方法和系统
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US11198900B2 (en) 2014-12-06 2021-12-14 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
EP3286326B1 (en) 2015-04-23 2025-01-22 Becton, Dickinson and Company Method for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
US11396650B2 (en) 2015-06-02 2022-07-26 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches
US10501777B2 (en) 2015-07-17 2019-12-10 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
CN114350752B (zh) 2015-07-17 2025-02-25 布鲁克空间生物学公司 在横切面组织的用户定义区域中的基因表达的同时定量
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
EP3913068B1 (en) 2015-10-12 2023-08-02 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
CN108474022A (zh) 2015-11-03 2018-08-31 哈佛学院董事及会员团体 用于包含三维核酸的基质容积成像的设备和方法
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
WO2017139409A1 (en) 2016-02-09 2017-08-17 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
US10919037B2 (en) 2016-03-23 2021-02-16 Children's Medical Center Corporation Systems and apparatus for detecting compounds in human biological samples
WO2017165647A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
EP3452614B1 (en) 2016-05-02 2023-06-28 Becton, Dickinson and Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
ES2908919T3 (es) 2016-07-05 2022-05-04 California Inst Of Techn Reacción en cadena de hibridación de iniciador fraccionario
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
AU2017359048B2 (en) 2016-11-08 2022-07-21 Becton, Dickinson And Company Methods for expression profile classification
CN109906274B (zh) 2016-11-08 2023-08-25 贝克顿迪金森公司 用于细胞标记分类的方法
AU2017361521B2 (en) 2016-11-21 2020-08-27 Bruker Spatial Biology, Inc. Chemical compositions and methods of using same
JP7093355B2 (ja) * 2016-12-16 2022-06-29 アラトム， エルエルシー ライゲーション増幅を使用する分子検出
US11492661B2 (en) * 2017-01-10 2022-11-08 President And Fellows Of Harvard College Multiplexed signal amplification
WO2018132610A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
CN107227350A (zh) * 2017-06-09 2017-10-03 苏州达麦迪生物医学科技有限公司 一种快速检测alk基因断裂的探针组、试剂盒及方法
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
WO2019068880A1 (en) 2017-10-06 2019-04-11 Cartana Ab RNA MATRIX LIGATION
US11591636B2 (en) 2017-11-20 2023-02-28 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
WO2019147945A1 (en) 2018-01-26 2019-08-01 President And Fellows Of Harvard College Proximity detection methods and compositions
JP7372927B6 (ja) 2018-02-12 2023-11-27 ナノストリング テクノロジーズ，インコーポレイティド 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
CN112292457A (zh) 2018-04-09 2021-01-29 领先细胞医疗诊断有限公司 用于原位检测核酸的进一步增强信号放大的方法
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
KR102893736B1 (ko) 2018-05-14 2025-11-28 브루커 스페이셜 바이올로지, 인크. 화학 조성물 및 이의 사용 방법
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
EP3894552B1 (en) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
CN114555825A (zh) 2019-02-15 2022-05-27 领先细胞医疗诊断有限公司 用于通过原位杂交进行核酸的多路复用检测的方法
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN119220641A (zh) 2019-05-31 2024-12-31 10X基因组学有限公司 检测目标核酸分子的方法
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
GB201919029D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
WO2021167986A1 (en) 2020-02-17 2021-08-26 10X Genomics, Inc. In situ analysis of chromatin interaction
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021174071A1 (en) 2020-02-29 2021-09-02 President And Fellows Of Harvard College Nanoswitch caliper trains for high-throughput, high-resolution structural analysis of biomolecules
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
WO2021207062A1 (en) 2020-04-07 2021-10-14 Advanced Cell Diagnostics, Inc. Methods for detecting target nucleic acids by in situ hybridization and a kit thereof
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
WO2022040455A1 (en) 2020-08-21 2022-02-24 Advanced Cell Diagnostics, Inc. Compositions and methods for determining t cell clonality
AU2021355407A1 (en) 2020-09-29 2023-05-18 Auburn University Dna-based assays
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284925A4 (en) 2021-01-26 2025-08-13 California Inst Of Techn ALLOSTERIC CONDITIONAL GUIDE RNAS FOR SELECTIVE REGULATION OF CRISPR/CAS CELLS
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
CN117460837A (zh) * 2021-05-24 2024-01-26 加州理工学院 与指数辐亮度拴系的连锁放大
EP4326900B1 (en) 2021-07-30 2024-09-04 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
EP4381095A1 (en) 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
US12674196B2 (en) 2022-01-18 2026-07-07 10X Genomics, Inc. Methods and compositions for improved probe specificity
EP4733410A3 (en) 2022-01-21 2026-05-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
US12703879B2 (en) 2022-03-29 2026-08-11 10X Genomics, Inc. RNase H assisted in situ rolling circle amplification
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
US12687494B2 (en) 2022-04-04 2026-07-21 10X Genomics, Inc. Systems and methods for nearly isotropic optical resolution using tilted structured illumination microscopy
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
US12692484B2 (en) 2022-08-12 2026-07-28 10X Genomics, Inc. PUMA1 polymerases and uses thereof
US12116626B2 (en) 2022-08-16 2024-10-15 10X Genomics, Inc. AP50 polymerases and uses thereof
WO2024124041A1 (en) 2022-12-07 2024-06-13 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5175270A (en) * 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
EP0481999B1 (en) * 1989-03-10 1997-07-23 VYSIS, Inc. Preventing interference with affinity capture schemes
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
AU3467293A (en) * 1991-12-23 1993-07-28 Chiron Corporation (Chlamydiae) probes for use in solution phase sandwich hybridization assays
ES2267094T3 (es) * 1991-12-23 2007-03-01 Novartis Vaccines And Diagnostics, Inc. Conjunto de sondas de vih para su uso en ensayos de hibridacion en sandwich en fase de disolucion.
EP0625214A4 (en) * 1991-12-23 1997-07-16 Chiron Corp Cmv probes for use in solution phase sandwich hybridization assays.
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes

Also Published As

Publication number Publication date
US5681697A (en) 1997-10-28
KR100327610B1 (ko) 2002-07-02
DE69432709D1 (de) 2003-06-26
US5635352A (en) 1997-06-03
KR960706566A (ko) 1996-12-09
HU221237B1 (en) 2002-08-28
JP3892450B2 (ja) 2007-03-14
JPH09507024A (ja) 1997-07-15
ATE241017T1 (de) 2003-06-15
EP0731848B1 (en) 2003-05-21
CA2178598A1 (en) 1995-06-15
AU694468B2 (en) 1998-07-23
HUT74225A (en) 1996-11-28
AU1303895A (en) 1995-06-27
HU9601593D0 (en) 1996-07-29
JP2004301849A (ja) 2004-10-28
WO1995016055A1 (en) 1995-06-15
EP0731848A1 (en) 1996-09-18
JP3802925B2 (ja) 2006-08-02
JP2006055170A (ja) 2006-03-02
ES2199236T3 (es) 2004-02-16
JP2006020649A (ja) 2006-01-26

Similar Documents

Publication Publication Date Title
US5681697A (en) Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
DE69233630T2 (de) Sätze von HIV-Sonden zur Verwendung in Sandwich-Hydridisierungsassays in Lösungsphase
DE69528839T2 (de) Reduzierung von nicht spezifischer hybridisierung unter verwendung neuer basenpaarschemata
DE68926504T2 (de) Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
EP0324474B1 (de) Verfahren zum Nachweis von Nukleinsäuren
DE3688931T2 (de) Arabinonukleinsäure-Sonden für DNS/RNS-Bestimmungen.
DE68927373T2 (de) Verfahren und mittel zum nachweis von nukleinsäuresequenzen
EP0608737B1 (de) Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren
DE69516116T2 (de) Sequenzierung von nukleinsäuren
DE69130612T2 (de) Grosse kammförmig verzweigte polynukleotide
EP0628568A2 (de) Verfahren zur Immobilisierung von Nukleinsäuren
EP1029077A2 (de) Spezifisches und empfindliches nukleinsäurenachweisverfahren
EP0523557B1 (de) Immobilisierung von Nukleinsäuren
EP0996631A1 (de) Modifizierte nukleinsäureanaloga
US7285401B1 (en) Method and reagents for detecting nucleic acid sequences
DE19814828A1 (de) Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19748690A1 (de) Spezifisches und sensitives Nukleinsäurenachweisverfahren
HK1006858B (en) Method and reagents for detecting nucleic acid sequences

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US

8328 Change in the person/name/address of the agent

Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN