KR100327610B1 - 백그라운드노이즈가감소된용액상핵산샌드위치측정법 - Google Patents

백그라운드노이즈가감소된용액상핵산샌드위치측정법 Download PDF

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KR100327610B1
KR100327610B1 KR1019960703018A KR19960703018A KR100327610B1 KR 100327610 B1 KR100327610 B1 KR 100327610B1 KR 1019960703018 A KR1019960703018 A KR 1019960703018A KR 19960703018 A KR19960703018 A KR 19960703018A KR 100327610 B1 KR100327610 B1 KR 100327610B1
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nucleic acid
capture
probe
hybridizing
support
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Korean (ko)
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KR960706566A (ko
Inventor
에스. 우데어 마이클
풀츠 티모시
디. 워너 브라이언
콜린스 마크
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유르데 미키
베이어 코퍼레이션
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
    • F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
    • F02B75/00—Other engines
    • F02B75/02—Engines characterised by their cycles, e.g. six-stroke
    • F02B2075/022—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle
    • F02B2075/027—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle four
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
KR1019960703018A 1993-12-08 1994-12-07 백그라운드노이즈가감소된용액상핵산샌드위치측정법 Expired - Fee Related KR100327610B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/164,388 US5681697A (en) 1993-12-08 1993-12-08 Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US08/164,388 1993-12-08
US08/164388 1993-12-08
PCT/US1994/014119 WO1995016055A1 (en) 1993-12-08 1994-12-07 Solution phase nucleic acid sandwich assays having reduced background noise

Publications (2)

Publication Number Publication Date
KR960706566A KR960706566A (ko) 1996-12-09
KR100327610B1 true KR100327610B1 (ko) 2002-07-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019960703018A Expired - Fee Related KR100327610B1 (ko) 1993-12-08 1994-12-07 백그라운드노이즈가감소된용액상핵산샌드위치측정법

Country Status (11)

Country Link
US (2) US5681697A (enExample)
EP (1) EP0731848B1 (enExample)
JP (4) JP3802925B2 (enExample)
KR (1) KR100327610B1 (enExample)
AT (1) ATE241017T1 (enExample)
AU (1) AU694468B2 (enExample)
CA (1) CA2178598A1 (enExample)
DE (1) DE69432709T2 (enExample)
ES (1) ES2199236T3 (enExample)
HU (1) HU221237B1 (enExample)
WO (1) WO1995016055A1 (enExample)

Families Citing this family (336)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7825215B1 (en) * 1993-04-26 2010-11-02 Peter E. Nielsen Substituted nucleic acid mimics
US7582421B2 (en) * 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
EP1260593A3 (en) * 1994-12-23 2003-12-03 Dade Behring Inc. Detection of nucleic acids by nuclease-catalyzed product formation
FR2737502B1 (fr) 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
DE19537952A1 (de) * 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
WO1997023646A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
WO1997023647A1 (en) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Homogeneous amplification and detection of nucleic acids
US5824478A (en) * 1996-04-30 1998-10-20 Vysis, Inc. Diagnostic methods and probes
AU4040097A (en) * 1996-07-12 1998-02-09 Tm Technologies, Inc. Signal amplification method
DK0975807T3 (da) * 1997-05-02 2007-01-29 Gen Probe Inc To-trins hybridisering og indfangning af et polynukleotid
US6534273B2 (en) 1997-05-02 2003-03-18 Gen-Probe Incorporated Two-step hybridization and capture of a polynucleotide
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US5939261A (en) * 1997-06-24 1999-08-17 Sarnoff Corporation Method for capturing a nucleic acid
JP2002508190A (ja) * 1997-12-12 2002-03-19 ダイジーン・コーポレーション 万能収集媒質
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
AU2117599A (en) 1998-01-20 1999-08-02 Mayo Foundation For Medical Education And Research Simplified mutation detection
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
GB9805935D0 (en) * 1998-03-19 1998-05-13 Ciba Geigy Ag Organic compounds
CA2335324A1 (en) * 1998-07-17 2000-01-27 Genetag Technology, Inc. Methods for detecting and mapping genes, mutations and variant polynucleotide sequences
EP0990903B1 (en) 1998-09-18 2003-03-12 Massachusetts Institute Of Technology Biological applications of semiconductor nanocrystals
US6203989B1 (en) * 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
US20030032029A1 (en) * 1998-12-21 2003-02-13 Nanogen, Inc. Three dimensional apparatus and method for integrating sample preparation and multiplex assays
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
ATE330032T1 (de) 1999-02-25 2006-07-15 Exact Sciences Corp Verfahren zur erhaltung der dns-integrität
GB9905580D0 (en) * 1999-03-12 1999-05-05 Tepnel Medical Ltd Enyzymatically catalysed signal amplification
CA2366778C (en) * 1999-04-09 2008-07-22 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
HK1046156B (en) 1999-04-20 2009-01-23 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20020045182A1 (en) * 1999-07-16 2002-04-18 Lynx Therapeutics, Inc. Multiplexed differential displacement for nucleic acid determinations
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6428957B1 (en) 1999-11-08 2002-08-06 Agilent Technologies, Inc. Systems tools and methods of assaying biological materials using spatially-addressable arrays
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
EP1238113B1 (en) * 1999-12-07 2010-02-24 EXACT Sciences Corporation Detection of lung neoplasms in stool
US6235483B1 (en) 2000-01-31 2001-05-22 Agilent Technologies, Inc. Methods and kits for indirect labeling of nucleic acids
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1259643B1 (en) 2000-02-07 2008-10-15 Illumina, Inc. Nucleic acid detection methods using universal priming
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US20020094538A1 (en) * 2001-01-13 2002-07-18 Getts Robert C. Methods for detecting and assaying nucleic acid sequences using temperature cycling
WO2001073122A1 (en) * 2000-03-28 2001-10-04 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US20030211496A1 (en) * 2000-03-29 2003-11-13 Getts Robert C Quality control reagents for nucleic acid microarrays
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
GB0016814D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (3)
GB0016813D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (4)
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
GB0016833D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
JP4965793B2 (ja) 2000-09-01 2012-07-04 ジェン−プローブ・インコーポレーテッド 薬剤耐性突然変異と関連する配列検出のためのｈｉｖ−１配列の増幅
US20030044803A1 (en) * 2000-09-22 2003-03-06 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of JAK1
US20020115058A1 (en) * 2000-09-22 2002-08-22 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of Pik3r1
US20020164576A1 (en) * 2000-09-22 2002-11-07 Pedersen Finn Skou Methods for diagnosis and treatment of diseases associated with altered expression of Nrf2
US6806051B2 (en) * 2000-09-25 2004-10-19 Picoliter Inc. Arrays of partially nonhybridizing oligonucleotides and preparation thereof using focused acoustic energy
AU2002213129A1 (en) * 2000-10-11 2002-04-22 William L. Ragland Methods and compositions using hybridization assays for detecting infectious agents
US7892730B2 (en) 2000-12-22 2011-02-22 Sagres Discovery, Inc. Compositions and methods for cancer
US7820447B2 (en) 2000-12-22 2010-10-26 Sagres Discovery Inc. Compositions and methods for cancer
US20030165878A1 (en) * 2000-12-22 2003-09-04 Morris David W. Novel compositions and methods in cancer associated with altered expression of MCM3AP
US20030232334A1 (en) 2000-12-22 2003-12-18 Morris David W. Novel compositions and methods for cancer
US7700274B2 (en) * 2000-12-22 2010-04-20 Sagres Discovery, Inc. Compositions and methods in cancer associated with altered expression of KCNJ9
US7645441B2 (en) * 2000-12-22 2010-01-12 Sagres Discovery Inc. Compositions and methods in cancer associated with altered expression of PRLR
US20030099963A1 (en) * 2000-12-22 2003-05-29 Morris David W. Novel compositions and methods in cancer associated with altered expression of TBX21
US20030087252A1 (en) * 2000-12-22 2003-05-08 Morris David W. Novel compositions and methods in cancer associated with altered expression of PRDM11
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US20030092157A1 (en) * 2001-03-16 2003-05-15 Hayden Michael R. Compositions, screening systems and methods for modulating HDL cholesterol and triglyceride levels
US20030191073A1 (en) 2001-11-07 2003-10-09 Challita-Eid Pia M. Nucleic acid and corresponding protein entitled 161P2F10B useful in treatment and detection of cancer
US20030082571A1 (en) * 2001-04-10 2003-05-01 Kachab Edward Hanna Linear nucleic acid and sequence therefor
CA2448484A1 (en) * 2001-05-25 2002-12-05 Xenon Genetics, Inc. Diagnostic methods for cardiovascular disease, low hdl-cholesterol levels, and high triglyceride levels
AU2002345746A1 (en) * 2001-06-21 2003-01-08 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
US20070098728A1 (en) * 2001-09-24 2007-05-03 Pedersen Finn S Novel compositions and methods in cancer
US20040126762A1 (en) * 2002-12-17 2004-07-01 Morris David W. Novel compositions and methods in cancer
US20040166490A1 (en) * 2002-12-17 2004-08-26 Morris David W. Novel therapeutic targets in cancer
US20040197778A1 (en) * 2002-12-26 2004-10-07 Sagres Discovery, Inc. Novel compositions and methods in cancer
US20060040262A1 (en) * 2002-12-27 2006-02-23 Morris David W Novel compositions and methods in cancer
US20040180344A1 (en) * 2003-03-14 2004-09-16 Morris David W. Novel therapeutic targets in cancer
US6946245B2 (en) * 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
US6635428B2 (en) * 2001-12-04 2003-10-21 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis B viral nucleic acids
CA2470774A1 (en) * 2001-12-19 2003-06-26 Hvidovre Hospital A method and a kit for determination of a microbial count
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
EP1481090A4 (en) * 2002-02-15 2006-08-09 Somalogic Inc METHOD AND REAGENTS FOR DETECTING BINDING OF TARGET MOLECULES BY NUCLEIC ACID ELECTRODES
EP2093233A1 (en) 2002-03-21 2009-08-26 Sagres Discovery, Inc. Novel compositions and methods in cancer
JP2005521410A (ja) * 2002-03-29 2005-07-21 ジェネンテック・インコーポレーテッド 核酸分析試料の検出と定量化のための方法及び組成物
EP1530581A4 (en) * 2002-06-12 2006-08-30 Datascope Investment Corp POLYMERIC MARKING MOLECULES
US7601493B2 (en) * 2002-07-26 2009-10-13 Nanogen, Inc. Methods and apparatus for screening and detecting multiple genetic mutations
EP1389638A1 (en) * 2002-08-06 2004-02-18 Roche Diagnostics GmbH Improved fluorescent resonance energy transfer probes
US20040081653A1 (en) 2002-08-16 2004-04-29 Raitano Arthur B. Nucleic acids and corresponding proteins entitled 251P5G2 useful in treatment and detection of cancer
US20060105337A1 (en) * 2002-08-30 2006-05-18 Brian Warner Solid phase based nucleic acid assays combining high affinity and high specificity
WO2004023142A1 (de) * 2002-09-03 2004-03-18 Zeptosens Ag Analytische plattform und nachweisverfahren mit den in einer probe nachzuweisenden analyten als immobilisierten spezifischen bindungspartnern
EP1567673A2 (en) * 2002-09-13 2005-08-31 Hvidovre Hospital Method of rapid detection of mutations and nucleotide polymorphisms using chemometrics
EP2323145A1 (en) * 2002-10-31 2011-05-18 Mitsubishi Chemical Corporation Electrolytic solution for electrolytic capacitor and electrolytic capacitor as well as method for preparing an organic onium tetrafluoroaluminate
WO2004061132A1 (en) * 2002-12-20 2004-07-22 Stratagene Compositions and methods for polynucleotide detection
AU2004210975B2 (en) 2003-02-10 2008-04-10 Agensys, Inc. Nucleic acid and corresponding protein named 158P1D7 useful in the treatment and detection of bladder and other cancers
US20040170982A1 (en) * 2003-02-14 2004-09-02 Morris David W. Novel therapeutic targets in cancer
US7767387B2 (en) * 2003-06-13 2010-08-03 Sagres Discovery, Inc. Therapeutic targets in cancer
US20070218071A1 (en) * 2003-09-15 2007-09-20 Morris David W Novel therapeutic targets in cancer
US20070149449A1 (en) 2003-02-14 2007-06-28 Morris David W Therapeutic targets in cancer
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
US7229763B2 (en) * 2003-04-07 2007-06-12 Beckman Coulter, Inc. Assay system using labeled oligonucleotides
EA009302B1 (ru) * 2003-05-20 2007-12-28 Инвестиген, Инк. Система обнаружения полинуклеотидов
DK1629088T3 (da) 2003-05-30 2012-05-07 Agensys Inc Varianter af prostatastamcelleantigen (psca) og undersekvenser deraf
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
US20040259100A1 (en) 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
DE20310332U1 (de) * 2003-07-04 2004-11-11 Mwg-Biotech Ag Vorrichtung zum automatischen Öffnen und Schließen von Reaktionsgefäßen
US20060035242A1 (en) * 2004-08-13 2006-02-16 Michelitsch Melissa D Prion-specific peptide reagents
WO2005040074A2 (en) * 2003-08-29 2005-05-06 Dendritic Nanotechnologies, Inc. Crick-watson base paired dendrimer structures
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
US20090163375A1 (en) 2003-09-09 2009-06-25 Bowman Christopher N Use of Photopolymerization for Amplification and Detection of a Molecular Recognition Event
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20070281896A1 (en) * 2003-09-30 2007-12-06 Morris David W Novel compositions and methods in cancer
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
WO2005111242A2 (en) * 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
US20080241827A1 (en) * 2004-05-10 2008-10-02 Exact Sciences Corporation Methods For Detecting A Mutant Nucleic Acid
EP2336363A1 (en) 2004-05-14 2011-06-22 Rosetta Genomics Ltd MicroRNAs and uses thereof
US20080124714A1 (en) * 2004-05-14 2008-05-29 Exact Sciences Corporation Method for Stabilizing Biological Samples for Nucleic Acid Analysis
JP5697297B2 (ja) 2004-05-14 2015-04-08 ロゼッタ ジノミクス リミテッド マイクロｎａｓおよびその使用
AU2005245996A1 (en) 2004-05-21 2005-12-01 Atonomics A/S Surface acoustic wave sensor comprising a hydrogel
JP4651663B2 (ja) 2004-05-28 2011-03-16 アジェンシス，インコーポレイテッド Ｐｓｃａタンパク質に結合する抗体および関連分子
JP5020818B2 (ja) 2004-07-01 2012-09-05 ジェン−プローブ・インコーポレーテッド 生物学的試料中の核酸を検出するための方法及び組成物
US20060024677A1 (en) 2004-07-20 2006-02-02 Morris David W Novel therapeutic targets in cancer
WO2006026654A2 (en) * 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US7482122B1 (en) * 2004-10-19 2009-01-27 Hai Xing Chen Method of signal enhancement for measurement of nucleic acid sequences
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
JP5188808B2 (ja) * 2004-11-03 2013-04-24 アイリス モレキュラー ダイアグノスティクス， インコーポレイテッド 同種の分析物検出
WO2006076683A2 (en) * 2005-01-13 2006-07-20 Novartis Vaccines And Diagnostics Inc. Isolation and detection of pathogenic prions
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
EP1909108B1 (en) 2005-03-10 2019-05-29 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifiying analytes
RU2413735C2 (ru) 2005-03-31 2011-03-10 Эдженсис, Инк. Антитела и родственные молекулы, связывающиеся с белками 161p2f10b
JP2008535853A (ja) 2005-04-07 2008-09-04 ノバルティス ヴァクシンズ アンド ダイアグノスティクス インコーポレイテッド 癌関連遺伝子
JP2008535857A (ja) 2005-04-07 2008-09-04 ノバルティス ヴァクシンズ アンド ダイアグノスティクス インコーポレイテッド 癌の診断、検出および処置におけるｃａｃｎａ１ｅ
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
ATE551433T1 (de) 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
US8628918B2 (en) 2005-05-09 2014-01-14 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1880025B1 (en) * 2005-05-12 2011-03-16 Affymetrix, Inc. Multiplex branched-chain dna assays
WO2007001986A2 (en) * 2005-06-20 2007-01-04 Yuling Luo Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
KR20080047355A (ko) * 2005-07-06 2008-05-28 바이오칩 이노베이션즈 피티와이 리미티드 표적 핵산 서열 분석 방법 및 키트
US20090137415A1 (en) * 2005-08-05 2009-05-28 Euclid Diagnostics Llc SUBTRACTIVE SEPARATION AND AMPLIFICATION OF NON-RIBOSOMAL TRANSCRIBED RNA (nrRNA)
US7410763B2 (en) * 2005-09-01 2008-08-12 Intel Corporation Multiplex data collection and analysis in bioanalyte detection
US20120231453A1 (en) * 2005-09-13 2012-09-13 Affymetrix, Inc. Brownian Microbarcodes for Bioassays
US7927798B2 (en) * 2005-10-05 2011-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
WO2007058898A2 (en) * 2005-11-10 2007-05-24 Panomics, Inc. Detection of nucleic acids through amplification of surrogate nucleic acids
US7537897B2 (en) * 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
US8021839B2 (en) * 2006-02-24 2011-09-20 Investigen, Inc. Methods and compositions for detecting polynucleotides
WO2007127564A2 (en) 2006-04-26 2007-11-08 Siemens Medical Solutions Diagnostics Gmbh Solid phase based nucleic acid assays combining high affinity capturing and detection by specific hybridization
DK2038432T3 (en) 2006-06-30 2017-04-03 Rosetta Genomics Ltd METHOD OF DETECTING AND QUANTIFYING A TARGET NUCLEIC ACID GENERATED BY RT-PCR BY MIRNA
JP2008043332A (ja) 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
JP2008064475A (ja) * 2006-09-04 2008-03-21 Osaka Univ 標的物質の高感度検出方法、検出用キットおよび検出装置
EP2407558A1 (en) * 2006-10-31 2012-01-18 Noxxon Pharma AG Methods for the detection of a single- or double-stranded nucleic acid molecule
WO2008069884A2 (en) 2006-12-01 2008-06-12 Panomics, Inc. Two-stage nucleic acid amplification using an amplification oligomer
US8481506B2 (en) * 2006-12-05 2013-07-09 Rosetta Genomics, Ltd. Nucleic acids involved in viral infection
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7855054B2 (en) * 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US20080241140A1 (en) * 2007-02-12 2008-10-02 Medical College Of Georgia Gene amplification of coactivator coaa and uses thereof
WO2008103702A2 (en) * 2007-02-23 2008-08-28 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
JP5211790B2 (ja) * 2007-03-26 2013-06-12 住友化学株式会社 Ｄｎａメチル化測定方法
US7794985B2 (en) * 2007-04-04 2010-09-14 Ghc Technologies, Inc. Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins
US20100047792A1 (en) * 2007-04-10 2010-02-25 Szendroe Istvan Label-free optical detection method
EP2180063B1 (en) * 2007-08-14 2013-01-09 Eisai R&D Management Co., Ltd. Method of detecting target substance
EP2511708B1 (en) 2007-10-05 2016-09-14 Affymetrix, Inc. Highly multiplexed particle-based assays
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
WO2009134942A1 (en) * 2008-04-30 2009-11-05 Novartis Ag. Assay for pathogenic conformers
EP2288726B1 (en) 2008-05-13 2012-06-13 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
US20090298709A1 (en) * 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
US8703416B2 (en) 2008-07-17 2014-04-22 Somalogic, Inc. Method for purification and identification of sperm cells
WO2010033844A2 (en) * 2008-09-22 2010-03-25 Biomicro, Inc. Selective processing of biological material on a microarray substrate
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
TWI394839B (zh) 2008-10-27 2013-05-01 Qiagen Gaithersburg Inc 快速結果雜交捕捉法及系統
US8309306B2 (en) * 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP2358897B1 (en) * 2008-11-17 2015-02-25 Headway Technologies, Inc. Methods and compositions in particle-based detection of target molecules using linking molecules
JP5744743B2 (ja) * 2008-11-17 2015-07-08 ヘッドウェイ テクノロジーズ, インク.Headway Technologies, Inc. 共有結合形成反応ペアを用いた標的分子の粒子ベースの検出における方法および組成物
ATE523603T1 (de) * 2008-11-21 2011-09-15 Chimera Biotec Gmbh Konjugatkomplexe zum analytnachweis
EP2367958B1 (en) * 2008-11-25 2017-08-23 Gen-Probe Incorporated Compositions and methods for detecting small rnas, and uses thereof
US8685646B2 (en) * 2008-12-18 2014-04-01 Siemens Healthcare Diagnostics Inc. Methods and reagents for shortening incubation times in hybridization assays
JP6108661B2 (ja) * 2009-01-28 2017-04-05 キアジェン ゲイサーズバーグ インコーポレイテッド 配列特異的な大量試料調製方法およびアッセイ法
WO2010096574A1 (en) 2009-02-20 2010-08-26 Lisanti Michael P A method of diagnosis or prognosis of a neoplasm comprising determining the level of expression of a protein in stromal cells adjacent to the neoplasm
JP5738278B2 (ja) 2009-05-01 2015-06-24 キアジェン ゲイサーズバーグ インコーポレイテッド 試料中のｒｎａスプライシング形態を検出するための非標的増幅法
AU2010291990B2 (en) 2009-09-14 2016-05-05 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
AU2010315133B2 (en) 2009-11-04 2015-09-10 Novartis Ag Positively charged species as binding reagents in the separation of protein aggregates from monomers
WO2011071923A2 (en) 2009-12-07 2011-06-16 Illumina, Inc. Multi-sample indexing for multiplex genotyping
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011084168A1 (en) * 2010-01-08 2011-07-14 The Regents Of The University Of California Bioassays based on polymeric sequence probes
US9605303B2 (en) 2010-01-29 2017-03-28 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of an HPV in a sample
EP2528932B1 (en) * 2010-01-29 2016-11-30 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
WO2011094669A1 (en) 2010-01-29 2011-08-04 Advanced Cell Diagnostics, Inc. Methods of in situ detection of nucleic acids
WO2011106583A1 (en) * 2010-02-26 2011-09-01 Ventana Medical Systems, Inc. Polytag probes
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
EP2576840B1 (en) 2010-05-25 2018-10-17 QIAGEN Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
ES2648564T3 (es) * 2010-10-21 2018-01-04 Advanced Cell Diagnostics, Inc. Método ultrasensible para la detección in situ de ácidos nucleicos
DK2633071T3 (en) 2010-10-27 2017-01-30 Harvard College COMPOSITIONS OF "MAINTENANCE" PRIMER DUPLEXES AND METHODS OF USE
US9255293B2 (en) 2010-11-01 2016-02-09 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
US20120178081A1 (en) * 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
CN103476951B (zh) 2011-02-16 2017-07-28 海德威技术公司 用于可检测的标记的靶标定位锚定的方法和组合物
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
WO2013067489A1 (en) * 2011-11-05 2013-05-10 President And Fellows Of Harvard College Nucleic acid-based linkers for detecting and measuring interactions
BR112014012288A2 (pt) 2011-11-22 2017-05-23 Intermune Inc métodos para diagnosticar e tratar fibrose pulmonar idiopática
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US10385392B2 (en) * 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes
US10085987B2 (en) 2012-01-27 2018-10-02 Thomas Jefferson University MCT protein inhibitor-related prognostic and therapeutic methods
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
WO2013128281A1 (en) 2012-02-28 2013-09-06 Population Genetics Technologies Ltd Method for attaching a counter sequence to a nucleic acid sample
EP2824180B1 (en) * 2012-03-05 2019-04-03 NGK Insulators, Ltd. Method for detecting target nucleic acid
US20140178869A1 (en) 2012-04-05 2014-06-26 Advanced Cell Diagnostics, Inc. Detection of immunoglobulin light chain restriction by rna in situ hybridization
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
WO2014074785A1 (en) 2012-11-08 2014-05-15 Ludwig Institute For Cancer Research Ltd. Methods of predicting outcome and treating breast cancer
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
US20160024565A1 (en) * 2013-03-13 2016-01-28 Tufts University Fragment complementation of based assays
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US9255265B2 (en) 2013-03-15 2016-02-09 Illumina, Inc. Methods for producing stranded cDNA libraries
EP2986290A4 (en) 2013-04-19 2017-01-04 Thomas Jefferson University Caveolin-1 related methods for treating glioblastoma with temozolomide
EP3434784A1 (en) * 2013-06-14 2019-01-30 Nanostring Technologies, Inc Multiplexable tag-based reporter system
US9745633B2 (en) * 2013-06-28 2017-08-29 Diacarta Ltd Detection of PNA clamping
DE102013011304A1 (de) * 2013-07-02 2015-01-22 Technische Universität Dresden Verfahren und Anordnung zur Erfassung von Bindungsereignissen von Molekülen
CN110964796B (zh) 2013-08-28 2024-04-05 贝克顿迪金森公司 大规模平行单细胞分析
JP2017504307A (ja) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド アレイ上のフィーチャーをデジタルカウントするための方法およびシステム
EP3378952B1 (en) 2013-12-28 2020-02-05 Guardant Health, Inc. Methods and systems for detecting genetic variants
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
WO2016089588A1 (en) 2014-12-06 2016-06-09 Children's Medical Center Corporation Nucleic acid-based linkers for detecting and measuring interactions
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
ES2975332T3 (es) 2015-02-19 2024-07-04 Becton Dickinson Co Análisis unicelular de alto rendimiento que combina información proteómica y genómica
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
WO2016196824A1 (en) 2015-06-02 2016-12-08 Children's Medical Center Corporation Nucleic acid complexes for screening barcoded compounds
US12077807B2 (en) 2015-06-27 2024-09-03 The Research Foundation For The State University Of New York Compositions and methods for analyte detection using nanoswitches
US10640816B2 (en) 2015-07-17 2020-05-05 Nanostring Technologies, Inc. Simultaneous quantification of gene expression in a user-defined region of a cross-sectioned tissue
SG10202107055SA (en) 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
US11078528B2 (en) 2015-10-12 2021-08-03 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
JP2019507585A (ja) 2015-12-17 2019-03-22 ガーダント ヘルス， インコーポレイテッド 無細胞ｄｎａの分析による腫瘍遺伝子コピー数を決定するための方法
WO2017139409A1 (en) 2016-02-09 2017-08-17 Children's Medical Center Corporation Method for detection of analytes via polymer complexes
WO2017165647A1 (en) 2016-03-23 2017-09-28 Children's Medical Center Corporation Rapid and sensitive detection and quantification of analytes in complex samples using polymer-based methods
US10919037B2 (en) 2016-03-23 2021-02-16 Children's Medical Center Corporation Systems and apparatus for detecting compounds in human biological samples
EP4613756A3 (en) * 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
EP4269616A3 (en) 2016-05-02 2024-02-14 Becton, Dickinson and Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
IL290679B2 (en) 2016-07-05 2023-10-01 California Inst Of Techn Hybridization chain reaction based on a minimal initiator
KR102638006B1 (ko) 2016-09-26 2024-02-20 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
CN109906274B (zh) 2016-11-08 2023-08-25 贝克顿迪金森公司 用于细胞标记分类的方法
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
JP6730525B2 (ja) 2016-11-21 2020-07-29 ナノストリング テクノロジーズ，インコーポレイティド 化学組成物とそれを利用する方法
DK3929304T3 (da) * 2016-12-16 2024-04-08 Aratome Llc Molekylær detektering ved ligeringsforstærkning
EP3568492B1 (en) * 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
CN110573253B (zh) 2017-01-13 2021-11-02 赛卢拉研究公司 流体通道的亲水涂层
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
WO2018165566A1 (en) 2017-03-09 2018-09-13 Auburn University Differential circuit for background correction in electrochemical measurements
EP4345172A3 (en) 2017-06-05 2024-07-03 Becton, Dickinson and Company Sample indexing for single cells
CN107227350A (zh) * 2017-06-09 2017-10-03 苏州达麦迪生物医学科技有限公司 一种快速检测alk基因断裂的探针组、试剂盒及方法
WO2019010341A1 (en) 2017-07-07 2019-01-10 Innamed, Inc. APTAMERS FOR MEASURING LIPOPROTEIN RATES
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
US11591636B2 (en) 2017-11-20 2023-02-28 Children's Medical Center Corporation Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
US11946095B2 (en) 2017-12-19 2024-04-02 Becton, Dickinson And Company Particles associated with oligonucleotides
EP3743525A4 (en) 2018-01-26 2021-09-29 President and Fellows of Harvard College PROXIMITY DETECTION METHODS AND COMPOSITIONS
KR20250161650A (ko) 2018-02-12 2025-11-17 브루커 스페이셜 바이올로지, 인크. 유전자 및 단백질 발현을 검출하는 생체 분자 프로브 및 방법
WO2019199643A1 (en) 2018-04-09 2019-10-17 Bio-Techne Corporation Methods to further enhance signal amplification for the in situ detection of nucleic acids
AU2019262048B2 (en) 2018-05-03 2025-09-04 Becton, Dickinson And Company High throughput multiomics sample analysis
EP4545647A3 (en) 2018-05-03 2025-07-09 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US20220154262A1 (en) 2019-02-15 2022-05-19 Advanced Cell Diagnostics, Inc. Methods for multiplex detection of nucleic acids by in situ hybridization
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN113906147B (zh) 2019-05-31 2024-09-13 10X基因组学有限公司 检测目标核酸分子的方法
EP4004231B1 (en) 2019-07-22 2025-11-12 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
EP4097228B1 (en) 2020-01-29 2024-08-14 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
JP2023514749A (ja) 2020-02-21 2023-04-07 １０エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4407030B1 (en) 2020-06-02 2025-12-17 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116194589A (zh) 2020-07-31 2023-05-30 贝克顿迪金森公司 用于转座酶可及染色质的单细胞测定
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
WO2022040455A1 (en) 2020-08-21 2022-02-24 Advanced Cell Diagnostics, Inc. Compositions and methods for determining t cell clonality
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284925A4 (en) 2021-01-26 2025-08-13 California Inst Of Techn ALLOSTERIC CONDITIONAL GUIDE RNAS FOR SELECTIVE REGULATION OF CRISPR/CAS CELLS
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
JP2024520987A (ja) * 2021-05-24 2024-05-28 カリフォルニア インスティチュート オブ テクノロジー 指数関数的な放射輝度と結びつく連鎖増幅
WO2023009842A1 (en) 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
CN117858958A (zh) 2021-08-16 2024-04-09 10X基因组学有限公司 包含分割条形码区的探针和使用方法
CN118974836A (zh) 2022-03-08 2024-11-15 10X基因组学有限公司 将光学拥挤最小化的原位代码设计方法
EP4505177A1 (en) 2022-04-01 2025-02-12 10x Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
WO2023245190A1 (en) 2022-06-17 2023-12-21 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2024040060A1 (en) 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
EP4630578A1 (en) 2022-12-07 2025-10-15 Advanced Cell Diagnostics, Inc. Multiplexed detection of nucleic acid targets
US12319956B2 (en) 2023-07-31 2025-06-03 10X Genomics, Inc. Methods and systems for targeted RNA cleavage and target RNA-primed rolling circle amplification

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5175270A (en) * 1986-09-10 1992-12-29 Polyprobe, Inc. Reagents for detecting and assaying nucleic acid sequences
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
DE69031121T2 (de) * 1989-03-10 1997-11-13 Vysis Inc Interferenzverhinderung mit affinitätseinfangschemas
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
DE69233630T2 (de) * 1991-12-23 2007-05-31 Chiron Corp., Emeryville Sätze von HIV-Sonden zur Verwendung in Sandwich-Hydridisierungsassays in Lösungsphase
CA2124795A1 (en) * 1991-12-23 1993-07-08 Ray Sanchez-Pescador Chlamydiae probes for use in solution phase sandwich hybridization assays
CA2124796A1 (en) * 1991-12-23 1993-07-08 Chiron Diagnostics Corporation Cmv probes for use in solution phase sandwich hybridization assays
US5424413A (en) * 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes

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EP0731848A1 (en) 1996-09-18
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JP2006020649A (ja) 2006-01-26
JP2004301849A (ja) 2004-10-28
JP3802925B2 (ja) 2006-08-02
US5635352A (en) 1997-06-03
AU694468B2 (en) 1998-07-23
US5681697A (en) 1997-10-28
WO1995016055A1 (en) 1995-06-15
JP3892450B2 (ja) 2007-03-14
JPH09507024A (ja) 1997-07-15
EP0731848B1 (en) 2003-05-21
JP2006055170A (ja) 2006-03-02
HU221237B1 (en) 2002-08-28
DE69432709T2 (de) 2003-11-20
KR960706566A (ko) 1996-12-09
DE69432709D1 (de) 2003-06-26
ATE241017T1 (de) 2003-06-15
AU1303895A (en) 1995-06-27
CA2178598A1 (en) 1995-06-15
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