DE60320520D1 - Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen - Google Patents

Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen

Info

Publication number
DE60320520D1
DE60320520D1 DE60320520T DE60320520T DE60320520D1 DE 60320520 D1 DE60320520 D1 DE 60320520D1 DE 60320520 T DE60320520 T DE 60320520T DE 60320520 T DE60320520 T DE 60320520T DE 60320520 D1 DE60320520 D1 DE 60320520D1
Authority
DE
Germany
Prior art keywords
amplification
serum
poxvirus
primary cells
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60320520T
Other languages
English (en)
Other versions
DE60320520T2 (de
DE60320520T3 (de
Inventor
Ingmar Raethe
Eva Felder
Karl Heller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bavarian Nordic AS
Original Assignee
Bavarian Nordic AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31970216&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60320520(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bavarian Nordic AS filed Critical Bavarian Nordic AS
Application granted granted Critical
Publication of DE60320520D1 publication Critical patent/DE60320520D1/de
Publication of DE60320520T2 publication Critical patent/DE60320520T2/de
Publication of DE60320520T3 publication Critical patent/DE60320520T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/275—Poxviridae, e.g. avipoxvirus
    • A61K39/285—Vaccinia virus or variola virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C12N7/08—Inactivation or attenuation by serial passage of virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Rheumatology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60320520.8T 2002-09-05 2003-09-01 Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen Expired - Lifetime DE60320520T3 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK200201302 2002-09-05
DKPA200201302 2002-09-05
PCT/EP2003/009704 WO2004022729A1 (en) 2002-09-05 2003-09-01 Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
EP03766057.8A EP1434858B2 (de) 2002-09-05 2003-09-01 Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen

Publications (3)

Publication Number Publication Date
DE60320520D1 true DE60320520D1 (de) 2008-06-05
DE60320520T2 DE60320520T2 (de) 2008-12-11
DE60320520T3 DE60320520T3 (de) 2019-05-09

Family

ID=31970216

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60320520.8T Expired - Lifetime DE60320520T3 (de) 2002-09-05 2003-09-01 Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen

Country Status (23)

Country Link
US (4) US7695939B2 (de)
EP (1) EP1434858B2 (de)
JP (2) JP2005537793A (de)
KR (1) KR101044538B1 (de)
CN (1) CN1692156B (de)
AT (1) ATE393212T2 (de)
AU (1) AU2003264144C1 (de)
BR (1) BRPI0313559B8 (de)
CA (1) CA2494379C (de)
CY (1) CY1114598T1 (de)
DE (1) DE60320520T3 (de)
DK (1) DK1434858T4 (de)
EA (1) EA009008B1 (de)
ES (1) ES2305514T5 (de)
HK (1) HK1080507B (de)
MX (1) MXPA04012966A (de)
NO (1) NO343489B1 (de)
NZ (1) NZ538575A (de)
PL (1) PL215169B1 (de)
PT (1) PT1434858E (de)
SI (1) SI1434858T2 (de)
UA (1) UA85543C2 (de)
WO (1) WO2004022729A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100537773C (zh) * 2000-11-23 2009-09-09 巴法里安诺迪克有限公司 改良安卡拉痘苗病毒变体
US7445924B2 (en) * 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
ATE393212T2 (de) * 2002-09-05 2008-05-15 Bavarian Nordic As Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen
EP1528101A1 (de) 2003-11-03 2005-05-04 ProBioGen AG Immortalisierte Vogel-Zelllinien für die Produktion von Viren
CA2593532C (en) 2005-02-23 2017-05-02 Bavarian Nordic A/S Use of a modified poxvirus for the rapid induction of immunity against a poxvirus or other infectious agents
FR2884255B1 (fr) * 2005-04-11 2010-11-05 Vivalis Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe
RU2489486C2 (ru) 2006-06-20 2013-08-10 Трансжене С.А. Процесс получения поксвирусов и композиции поксвирусов
SG178090A1 (en) 2009-07-21 2012-03-29 Transgene Sa Enzymatic composition for the digestion of chicken embryos
WO2011103137A1 (en) * 2010-02-17 2011-08-25 Alnylam Pharmaceuticals, Inc. Cell-based methods and reagents
BR112012026095A2 (pt) 2010-04-14 2015-09-15 Emd Millipore Corp métodos de produção de estoques de vírus de alta pureza e altos títulos e métodos de uso dos mesmos.
AU2011281982B2 (en) * 2010-07-20 2015-12-17 Bavarian Nordic A/S Method for harvesting expression products
KR101504159B1 (ko) * 2012-04-20 2015-03-19 한국생명공학연구원 미세조류의 성장을 촉진하는 엑소피아라 올리고스퍼마 및 이의 용도
WO2015195758A1 (en) * 2014-06-18 2015-12-23 Medimmune, Llc Cell culture methods and media comprising n-acetylcysteine
KR101677231B1 (ko) 2014-12-18 2016-11-30 제주대학교 산학협력단 무혈청배지（serum free medium）를 이용한 메갈로사이티바이러스의 효율적 제조방법
CN105462911B (zh) * 2015-12-22 2018-04-13 肇庆大华农生物药品有限公司 用于培养病毒的无血清培养液及其制备方法
CN107129963A (zh) * 2017-04-24 2017-09-05 浙江美保龙生物技术有限公司 一种df‑1细胞培养液添加剂及其制备方法、使用方法
CA3061678A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
CA3062549A1 (en) 2017-05-15 2018-11-22 Janssen Vaccines & Prevention B.V. Stable virus-containing composition
US20210301263A1 (en) * 2018-08-08 2021-09-30 Arizona Board Of Regents On Behalf Of Arizona State University Methods for improved poxvirus yields
WO2020049151A1 (en) 2018-09-06 2020-03-12 Bavarian Nordic A/S Storage improved poxvirus compositions
BR112022017438A2 (pt) 2020-03-12 2022-10-18 Bavarian Nordic As Composições que melhoram a estabilidade do poxvírus
CN113355297A (zh) * 2021-06-23 2021-09-07 吉林冠界生物技术有限公司 一种灌流式培养全悬浮mdck细胞生产重组禽流感病毒的方法
WO2024003238A1 (en) 2022-06-29 2024-01-04 Bavarian Nordic A/S Epstein-barr-virus vaccine
WO2024003239A1 (en) 2022-06-29 2024-01-04 Bavarian Nordic A/S RECOMBINANT MODIFIED saRNA (VRP) AND VACCINIA VIRUS ANKARA (MVA) PRIME-BOOST REGIMEN
CN116656628A (zh) * 2023-07-31 2023-08-29 深圳市卫光生物制品股份有限公司 一种痘病毒载体疫苗的制备方法

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2004402A (en) * 1934-04-07 1935-06-11 Conklin John Edward Atomizer
US3887430A (en) * 1972-02-24 1975-06-03 Dow Chemical Co Culture medium for tissue culture techniques
US4072565A (en) * 1974-11-04 1978-02-07 The Dow Chemical Company Production of viruses in tissue culture without use of serum
US3914408A (en) * 1973-10-12 1975-10-21 Univ Nebraska Vaccine for neonatal calf diarrhea
DE2714665A1 (de) * 1977-04-01 1978-10-05 Mayr Anton Praeparat zur behandlung von herpes zoster und anderen herpes-infektionen, sowie verfahren zu seiner herstellung
US5338683A (en) * 1981-12-24 1994-08-16 Health Research Incorporated Vaccinia virus containing DNA sequences encoding herpesvirus glycoproteins
US5155020A (en) * 1989-03-08 1992-10-13 Health Research Inc. Recombinant poxvirus host range selection system
US5833975A (en) * 1989-03-08 1998-11-10 Virogenetics Corporation Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence
AU613583B2 (en) * 1986-08-22 1991-08-08 Transgene S.A. Proteins and the method for preparing them, DNA sequences, antibodies and their application, poxviruses, transformed or infected cells, pharmaceutical compositions which are useful in the prevention of schistosomiasis and application by way of an agent possessing glutathione s-transferase activity
US5024947A (en) * 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
CA1341245C (en) * 1988-01-12 2001-06-05 F. Hoffmann-La Roche Ag Recombinant vaccinia virus mva
US6248333B1 (en) 1990-04-04 2001-06-19 Health Research Inc. Isolated nucleic acid sequence of equine herpesvirus type 1 glycoprotein D (EHV-1 gD)
AT393277B (de) * 1990-01-04 1991-09-25 Immuno Ag Verfahren zur herstellung von fruehsommer- meningoenzephalitis-virus (fsme-virus)-antigen
CA2091443A1 (en) * 1990-09-25 1992-03-26 Luciano Ramos Medium for culture of mammalian cells
WO1992015672A1 (en) * 1991-03-07 1992-09-17 Virogenetics Corporation Genetically engineered vaccine strain
US5843456A (en) * 1991-03-07 1998-12-01 Virogenetics Corporation Alvac poxvirus-rabies compositions and combination compositions and uses
US5766598A (en) 1991-03-07 1998-06-16 Virogenetics Corporation Recombinant attenuated ALVAC canarypoxvirus expression vectors containing heterologous DNA segments encoding lentiviral gene products
IL102929A (en) 1992-08-24 1996-11-14 Interpharm Lab Ltd Serum-free medium for mammalian cells
US5403582A (en) * 1993-01-21 1995-04-04 Nippon Zeon Co., Ltd. Vaccine comprising fowlpox virus recombinants expressing the envelope glycoprotein of an avian reticuloendotheliosis retrovirus
US5405772A (en) 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
US5768598A (en) 1993-09-13 1998-06-16 Intel Corporation Method and apparatus for sharing hardward resources in a computer system
US6015686A (en) * 1993-09-15 2000-01-18 Chiron Viagene, Inc. Eukaryotic layered vector initiation systems
US6190655B1 (en) * 1993-12-03 2001-02-20 Immunex Corporation Methods of using Flt-3 ligand for exogenous gene transfer
DE4405841C1 (de) * 1994-02-23 1995-01-05 Mayr Anton Prof Dr Med Vet Dr Multipotente Paramunitätsinducer auf der Basis von Kombinationen von Pockenviruskomponenten, Verfahren zu ihrer Herstellung und ihre Verwendung als Arzneimittel
EP0758397B1 (de) * 1994-04-29 2005-06-22 Baxter Healthcare S.A. Rekombinante poxviren mit fremdenpolynukleotiden in wichtigen regionen
US5756341A (en) * 1994-11-10 1998-05-26 Immuno Ag Method for controlling the infectivity of viruses
US6146873A (en) * 1994-11-10 2000-11-14 Baxter Aktiengesellschaft Production of orthomyxoviruses in monkey kidney cells using protein-free media
US5753489A (en) * 1994-11-10 1998-05-19 Immuno Ag Method for producing viruses and vaccines in serum-free culture
DK0791055T3 (da) 1994-11-10 2012-04-16 Baxter Healthcare Sa Fremgangsmåde til fremstilling af biologiske produkter i proteinfri kultur
US5503582A (en) 1994-11-18 1996-04-02 Micron Display Technology, Inc. Method for forming spacers for display devices employing reduced pressures
UA68327C2 (en) 1995-07-04 2004-08-16 Gsf Forschungszentrum Fur Unwe A recombinant mva virus, an isolated eukaryotic cell, infected with recombinant mva virus, a method for production in vitro of polypeptides with use of said cell, a method for production in vitro of virus parts (variants), vaccine containing the recombinant mva virus, a method for immunization of animals
JPH09154571A (ja) * 1995-12-05 1997-06-17 Kurabo Ind Ltd ウイルス製造用無血清培地
EP0882134B8 (de) 1996-02-21 2009-09-02 Genetic Immunity kft. Verfahren und zusammensetzungen zur genetisch schützenden und therapeutischen immunisierung
AU3813597A (en) 1996-07-26 1998-02-20 University Of Manitoba Serum-free medium for growth of anchorage-dependant mammalian cells
ID19548A (id) 1996-09-24 1998-07-23 Bavarian Nordic Res Inst As Virus mva rekombinan yang mengekspresikan antigen-antigen virus demam dan penggunaan daripadanya dalam vaksin-vaksin
AU4751697A (en) 1996-10-10 1998-05-05 Douglas Danner Animal cell culture media comprising plant-derived nutrients
WO1998017283A1 (en) 1996-10-25 1998-04-30 The Wistar Institute Of Anatomy & Biology Method of vaccinating infants against infections
US6204250B1 (en) * 1996-11-22 2001-03-20 The Mount Sinai Medical Center Of The City Of New York Immunization of infants
US20030138454A1 (en) * 1997-06-09 2003-07-24 Oxxon Pharmaccines, Ltd. Vaccination method
GB9711957D0 (en) 1997-06-09 1997-08-06 Isis Innovation Methods and reagents for vaccination
GB0023203D0 (en) 2000-09-21 2000-11-01 Isis Innovation Vaccination method
AT407255B (de) * 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
AU3910097A (en) 1997-08-05 1999-03-01 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Live recombinant vaccine comprising inefficiently or non-replicating vir us
WO2000008179A1 (en) * 1998-08-04 2000-02-17 Immunex Corporation Ikr-1 and ikr-2, protein kinases which are related to the i kappa b kinases
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
DE69839970D1 (de) 1998-11-10 2008-10-16 Univ Rochester Methoden zu herstellung von genbanken
EP1160323A1 (de) 1998-11-18 2001-12-05 Oxford Biomedica (UK) Limited Verwendung vom Krebs-assoziierten Antigen 5T4 zur Immuntherapie
GB2370571B (en) 1998-11-18 2003-05-07 Oxford Biomedica Ltd A modified 5t4 antigen
US6497883B1 (en) * 1999-06-10 2002-12-24 Merial Porcine circovirus recombinant poxvirus vaccine
DE19935351A1 (de) 1999-07-29 2001-02-01 Abb Daimler Benz Transp Verfahren zur Energieoptimierung bei einem Fahrzeug/Zug mit arbeitspunktabhängigem Wirkungsgrad
DK1263936T3 (da) * 2000-03-14 2006-02-13 Bavarian Nordic As andret stamme af den modificerede Vacciniavirus Ankara (MVA)
EP1283718A2 (de) 2000-05-24 2003-02-19 Merial Impfstoff aus rekombinantem geflügelpockenvirus gegen fortpflanzungs- und atmungs-syndrom schweinevirus
PL366397A1 (en) * 2000-07-11 2005-01-24 Bayer Aktiengesellschaft Use of strains of the parapox ovis virus for producing antiviral pharmaceuticals and anticancer pharmaceuticals
DK1358319T3 (da) * 2000-09-25 2009-10-05 Polymun Scient Immunbio Forsch Levende influenzavaccine og fremgangsmåde til fremstilling heraf
US7445924B2 (en) * 2000-11-23 2008-11-04 Bavarian Nordic A/S Modified Vaccinia Ankara virus variant and cultivation method
CN100537773C (zh) 2000-11-23 2009-09-09 巴法里安诺迪克有限公司 改良安卡拉痘苗病毒变体
US7097842B2 (en) * 2000-11-23 2006-08-29 Bavarian Nordic A/S Modified vaccinia virus ankara for the vaccination of neonates
UA82466C2 (uk) * 2001-07-18 2008-04-25 Бавариан Нордика А/С Спосіб посилення ампліфікації хордопоксвірусу
KR101042458B1 (ko) * 2001-12-04 2011-06-16 벤처 테크놀로지스 에스디엔 비에이치디 플라비바이러스 ｎｓ1 서브유닛 백신
US6951752B2 (en) 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
BR0214822A (pt) 2001-12-10 2004-12-14 Bavarian Nordic As Formulações contendo poxvìrus e processo para preparar composições contendo poxvìrus estáveis
UA77735C2 (en) * 2001-12-20 2007-01-15 Bavarian Nordic As Method for recovery of poxviruses from infected cells
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
AU2003219057A1 (en) * 2002-03-15 2003-09-29 Baxter Healthcare S.A. Recombinant drug-sensitive vaccinia virus as smallpox vaccine
JP4895505B2 (ja) * 2002-05-16 2012-03-14 バヴァリアン・ノルディック・アクティーゼルスカブ 変異ワクシニアウイルスアンカラ（ｍｖａ）ゲノム中の挿入部位としての遺伝子間領域
KR20040108804A (ko) * 2002-05-16 2004-12-24 버베리안 노딕 에이/에스 우두 ａｔｉ 프로모터를 사용하는 것에 의한 변형백시니아 바이러스 안카라에서 유전자의 발현
ATE393212T2 (de) 2002-09-05 2008-05-15 Bavarian Nordic As Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen
ATE467678T1 (de) 2003-07-22 2010-05-15 Vivalis Produktion von poxviren mit adhärenten oder nicht adhärenten vogelzellinien
US6915752B2 (en) * 2003-09-30 2005-07-12 Lockheed Martin Corporation Utility service protection strip
US6976752B2 (en) * 2003-10-28 2005-12-20 Lexmark International, Inc. Ink jet printer with resistance compensation circuit

Also Published As

Publication number Publication date
CA2494379A1 (en) 2004-03-18
HK1080507B (zh) 2011-10-07
EP1434858A1 (de) 2004-07-07
JP2005537793A (ja) 2005-12-15
PL373992A1 (en) 2005-09-19
CY1114598T1 (el) 2016-10-05
PT1434858E (pt) 2008-07-28
US8329466B2 (en) 2012-12-11
ES2305514T5 (es) 2019-06-14
US20050214324A1 (en) 2005-09-29
EP1434858B1 (de) 2008-04-23
WO2004022729A8 (en) 2005-03-17
US8673318B2 (en) 2014-03-18
ES2305514T3 (es) 2008-11-01
AU2003264144A2 (en) 2004-03-29
AU2003264144A1 (en) 2004-03-29
AU2003264144C1 (en) 2009-12-17
DE60320520T2 (de) 2008-12-11
SI1434858T2 (sl) 2019-05-31
EA009008B1 (ru) 2007-10-26
NO343489B1 (no) 2019-03-25
EA200500448A1 (ru) 2005-08-25
US7964397B2 (en) 2011-06-21
DK1434858T4 (en) 2019-04-01
HK1080507A1 (en) 2006-04-28
DE60320520T3 (de) 2019-05-09
US20130089913A1 (en) 2013-04-11
WO2004022729A1 (en) 2004-03-18
KR101044538B1 (ko) 2011-06-27
AU2003264144B2 (en) 2009-05-14
EP1434858B2 (de) 2018-12-19
ATE393212T2 (de) 2008-05-15
KR20050037551A (ko) 2005-04-22
JP5612338B2 (ja) 2014-10-22
UA85543C2 (ru) 2009-02-10
CN1692156A (zh) 2005-11-02
CN1692156B (zh) 2011-05-11
US7695939B2 (en) 2010-04-13
US20090029459A1 (en) 2009-01-29
DK1434858T3 (da) 2008-08-11
BRPI0313559B8 (pt) 2021-05-25
MXPA04012966A (es) 2005-05-16
US20110217749A1 (en) 2011-09-08
SI1434858T1 (sl) 2008-10-31
CA2494379C (en) 2012-11-06
BRPI0313559B1 (pt) 2019-02-26
BR0313559A (pt) 2005-07-12
PL215169B1 (pl) 2013-10-31
NO20051175L (no) 2005-03-04
JP2010148522A (ja) 2010-07-08
NZ538575A (en) 2007-03-30

Similar Documents

Publication Publication Date Title
ATE393212T2 (de) Verfahren zur amplifikation von einem poxvirus in serumfreien verhältnissen
DK1646715T3 (da) Fremstilling af poxvirum med adhærente eller ikke-adhærente fuglecellelinjer
JP2003526362A5 (de)
DK1108787T3 (da) Fremstilling af vacciner
ATE517996T1 (de) Verfahren und mittel zur erhöhung der toleranz von pflanzen gegenüber stressbedingungen
DK0904351T3 (da) Dyreceller og fremgangsmåder til replikationen af influenzavirus
NO20054124L (no) Dyre-fri celle kultur metode
RU2011148791A (ru) Иммортализованные линии клеток птиц и их применение
ATE289348T1 (de) Methoden zur erhöhung der produktion von viralen impstoffen in zellkultur durch unterdrückung von interferon
DE50214416D1 (de) Vermehrung von viren in zellkultur
ATE464375T1 (de) Verfahren zur herstellung von virusmaterial
ATE530657T1 (de) Verfahren zur herstellung von zellinien mit latentem immunschwächevirus
ATE270325T1 (de) Rückgewinnung von viren aus zellkulturen durch verwendung einer hypertonischen salzlösung
ATE511397T1 (de) Lungenzellen von baumwollratten zur virenkultur
ATE486124T1 (de) Keratinozyten-kulturverfahren und verwendung davon
ATE313622T1 (de) Verfahren zur züchtung von haftenden tierischen zellen
WO2002028174A1 (fr) Procedes d'infection par le virus de l'hepatite c
MX2021006115A (es) Medio libre de suero para produccion de vacuna aviar y usos del mismo.

Legal Events

Date Code Title Description
8363 Opposition against the patent