ATE530657T1 - Verfahren zur herstellung von zellinien mit latentem immunschwächevirus - Google Patents
Verfahren zur herstellung von zellinien mit latentem immunschwächevirusInfo
- Publication number
- ATE530657T1 ATE530657T1 AT02797433T AT02797433T ATE530657T1 AT E530657 T1 ATE530657 T1 AT E530657T1 AT 02797433 T AT02797433 T AT 02797433T AT 02797433 T AT02797433 T AT 02797433T AT E530657 T1 ATE530657 T1 AT E530657T1
- Authority
- AT
- Austria
- Prior art keywords
- immunodeficiency virus
- latent
- invention further
- further provides
- cell lines
- Prior art date
Links
- 206010061598 Immunodeficiency Diseases 0.000 title abstract 8
- 208000029462 Immunodeficiency disease Diseases 0.000 title abstract 8
- 230000007813 immunodeficiency Effects 0.000 title abstract 8
- 241000700605 Viruses Species 0.000 title abstract 7
- 238000004519 manufacturing process Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 210000004027 cell Anatomy 0.000 abstract 3
- 238000012216 screening Methods 0.000 abstract 2
- 230000006044 T cell activation Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000007420 reactivation Effects 0.000 abstract 1
- 230000001177 retroviral effect Effects 0.000 abstract 1
- 238000007423 screening assay Methods 0.000 abstract 1
- 230000009385 viral infection Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/60—Vector systems having a special element relevant for transcription from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US34172701P | 2001-12-19 | 2001-12-19 | |
PCT/US2002/040698 WO2003054160A2 (en) | 2001-12-19 | 2002-12-18 | Cell lines with latent immunodeficiency virus and methods of use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE530657T1 true ATE530657T1 (de) | 2011-11-15 |
Family
ID=23338764
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02797433T ATE530657T1 (de) | 2001-12-19 | 2002-12-18 | Verfahren zur herstellung von zellinien mit latentem immunschwächevirus |
Country Status (5)
Country | Link |
---|---|
US (3) | US7232685B2 (de) |
EP (1) | EP1463822B1 (de) |
AT (1) | ATE530657T1 (de) |
AU (1) | AU2002361797A1 (de) |
WO (1) | WO2003054160A2 (de) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003303953B2 (en) * | 2002-09-10 | 2007-10-04 | Microbiotix, Inc. | Antibacterial pyrazole carboxylic acid hydrazides |
CN1301263C (zh) * | 2002-12-18 | 2007-02-21 | 北京昭衍新药研究中心 | 一组抗hiv感染及防治艾滋病的核苷酸序列及其应用 |
WO2006019841A2 (en) * | 2004-07-13 | 2006-02-23 | Government Of The United States Of America | Treatment of viral infections with egr 1 activators |
WO2006029029A2 (en) * | 2004-09-02 | 2006-03-16 | The Uab Research Foundation | Compositions for detection of latent hiv reactivation and methods of using the same |
CA2632456C (en) * | 2005-12-05 | 2016-06-21 | Joseph P. Dougherty | An hiv-1 latency model for high throughput screening |
EP2010680A2 (de) * | 2006-04-14 | 2009-01-07 | Tibotec Pharmaceuticals Ltd. | Verfahren und mittel zur beurteilung von hiv-gag-/proteasehemmer-therapie |
GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
WO2009018219A2 (en) | 2007-07-28 | 2009-02-05 | University Of Chicago | Methods and compositions for modulating rad51 and homologous recombination |
JP5779503B2 (ja) * | 2008-10-29 | 2015-09-16 | イスティチュート スペリオーレ ディ サニータ | 酸化ストレスを用いるレトロウイルスレゼルボアの処置 |
WO2010099169A2 (en) * | 2009-02-24 | 2010-09-02 | Johns Hopkins University | A novel in vitro hiv-1 latency model for screening reactivation agents of hiv-1 |
US9474788B2 (en) | 2010-05-10 | 2016-10-25 | The J. David Gladstone Institutes | Methods for the reactivation of latent HIV using cytosine methylation inhibitors and NF-KB activators |
EP2830629A4 (de) * | 2012-03-28 | 2016-01-20 | Icahn School Of Medicine Mount Sinai | Zusammensetzungen und verfahren zur reaktivierung von latentem immundefizienzvirus |
US10106818B2 (en) | 2013-08-16 | 2018-10-23 | The J. David Gladstone Institutes | Dual-color HIV reporter system for the detection of latently-infected cells |
WO2017096161A1 (en) * | 2015-12-04 | 2017-06-08 | The J. David Gladstone Institutes | Compositions and methods for reactivating latent immunodeficiency virus using an akt activator |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5459056A (en) * | 1989-08-28 | 1995-10-17 | The United States Of America As Represented By The Department Of Health And Human Services | Human T cell line chronically infected with HIV |
US5256534A (en) | 1991-08-09 | 1993-10-26 | The United States Of America As Represented By The Department Of Health And Human Services | CD4+, latently HIV-1-infected hematopoietic progenitor cells |
US6025124A (en) | 1994-03-03 | 2000-02-15 | The Regents Of The University Of California | Marker, method and an assay for detection and monitoring of latency and activation of human immunodeficiency virus |
WO1999016891A1 (en) | 1997-10-01 | 1999-04-08 | Oregon Health Sciences University | Methods of replicating virus in monocyte-derived macrophage cultures |
-
2002
- 2002-12-18 AT AT02797433T patent/ATE530657T1/de not_active IP Right Cessation
- 2002-12-18 AU AU2002361797A patent/AU2002361797A1/en not_active Abandoned
- 2002-12-18 US US10/323,463 patent/US7232685B2/en active Active
- 2002-12-18 EP EP02797433A patent/EP1463822B1/de not_active Expired - Lifetime
- 2002-12-18 WO PCT/US2002/040698 patent/WO2003054160A2/en not_active Application Discontinuation
-
2007
- 2007-05-09 US US11/746,404 patent/US7544467B2/en not_active Expired - Lifetime
- 2007-10-29 US US11/927,262 patent/US20090306131A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1463822A4 (de) | 2005-12-28 |
US7232685B2 (en) | 2007-06-19 |
AU2002361797A1 (en) | 2003-07-09 |
WO2003054160A3 (en) | 2003-12-18 |
AU2002361797A8 (en) | 2003-07-09 |
WO2003054160A2 (en) | 2003-07-03 |
US20030157693A1 (en) | 2003-08-21 |
EP1463822A2 (de) | 2004-10-06 |
US7544467B2 (en) | 2009-06-09 |
EP1463822B1 (de) | 2011-10-26 |
US20090306131A1 (en) | 2009-12-10 |
US20080038716A1 (en) | 2008-02-14 |
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Legal Events
Date | Code | Title | Description |
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |