DE602005025791D1 - Verfahren zur nukleinsäureamplifikation - Google Patents

Verfahren zur nukleinsäureamplifikation

Info

Publication number
DE602005025791D1
DE602005025791D1 DE602005025791T DE602005025791T DE602005025791D1 DE 602005025791 D1 DE602005025791 D1 DE 602005025791D1 DE 602005025791 T DE602005025791 T DE 602005025791T DE 602005025791 T DE602005025791 T DE 602005025791T DE 602005025791 D1 DE602005025791 D1 DE 602005025791D1
Authority
DE
Germany
Prior art keywords
pcr
late
following
detection during
fluorescent dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602005025791T
Other languages
German (de)
English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Cristina Hartshorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36203686&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602005025791(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Brandeis University filed Critical Brandeis University
Publication of DE602005025791D1 publication Critical patent/DE602005025791D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE602005025791T 2004-10-18 2005-10-17 Verfahren zur nukleinsäureamplifikation Expired - Lifetime DE602005025791D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

Publications (1)

Publication Number Publication Date
DE602005025791D1 true DE602005025791D1 (de) 2011-02-17

Family

ID=36203686

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005025791T Expired - Lifetime DE602005025791D1 (de) 2004-10-18 2005-10-17 Verfahren zur nukleinsäureamplifikation

Country Status (17)

Country Link
US (3) US7632642B2 (https=)
EP (3) EP1805199B1 (https=)
JP (2) JP5543065B2 (https=)
KR (3) KR101424812B1 (https=)
CN (1) CN101076607B (https=)
AT (1) ATE494295T1 (https=)
AU (1) AU2005295298B2 (https=)
BR (1) BRPI0517105B1 (https=)
CA (3) CA2996947C (https=)
DE (1) DE602005025791D1 (https=)
DK (1) DK1805199T3 (https=)
ES (3) ES2359322T3 (https=)
NO (1) NO20072506L (https=)
PL (1) PL1805199T3 (https=)
PT (1) PT1805199E (https=)
RU (1) RU2460804C2 (https=)
WO (1) WO2006044994A2 (https=)

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2460804C2 (ru) 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
US7933011B2 (en) 2007-07-12 2011-04-26 Smiths Detection Inc. Fiber optic detection system
WO2009055617A1 (en) 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
US9856522B2 (en) 2008-12-30 2018-01-02 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムpcr装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
AU2010224100B2 (en) * 2009-03-12 2015-10-22 Brandeis University Reagents and methods for PCR
US8674080B2 (en) * 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
US8853373B2 (en) * 2009-08-14 2014-10-07 Hitachi, Ltd. Method and reagent for gene sequence analysis
ES2743959T3 (es) 2009-10-21 2020-02-21 Univ Brandeis Kits para analizar secuencias de ácido nucleico monocatenario
CA2778249C (en) * 2009-11-03 2018-12-04 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection sequencing (qnps)
EP2569447A4 (en) * 2010-05-14 2013-11-27 Fluidigm Corp ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
WO2012024642A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Quantitative real-time pcr assay using fret dual-labeled primers
US9850529B2 (en) 2010-09-08 2017-12-26 Brandeis University Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
EP2619329B1 (en) 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
WO2012048207A2 (en) * 2010-10-07 2012-04-12 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP3135775B1 (en) 2010-11-10 2018-06-27 Brandeis University Kit for detecting and identifying mycobacteria
EP2646575B1 (en) 2010-12-03 2017-05-31 Brandeis University Detecting mutations in dna
WO2012079016A1 (en) * 2010-12-10 2012-06-14 Brandeis University Compositions and methods for the detection and analysis of african swine fever virus
WO2012092259A1 (en) 2010-12-27 2012-07-05 Ibis Biosciences, Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
ES2597032T3 (es) 2011-05-04 2017-01-13 HTG Molecular Diagnostics, Inc Mejoras en un ensayo cuantitativo de protección contra la nucleasa (qNPA) y secuenciación (qNPS)
US20150031024A1 (en) * 2011-07-30 2015-01-29 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
CN103946398B (zh) 2011-09-15 2019-08-13 健能泰格技术公司 探针:反义探针组合物对高特异性dna或rna的检测
CN104024433B (zh) * 2011-10-31 2016-11-16 爱科来株式会社 基因丰度的测定方法
CN114717296B (zh) 2012-02-03 2025-04-04 加州理工学院 多路生化测定中信号的编码和解码
WO2013131083A1 (en) 2012-03-02 2013-09-06 Winthrop-University Hospital METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED β CELL DERIVED DNA AS A MEASURE OF β CELL LOSS IN DIABETES
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
EP3901243A1 (en) * 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
CN104995312A (zh) * 2012-09-17 2015-10-21 布兰代斯大学 用于表征ssdna序列的dsdna结合染料和探针的组合
EP2959016A4 (en) * 2013-02-21 2016-10-12 Toma Biosciences Inc METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS
EP2971095B1 (en) 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
CN105378160B (zh) 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
CN105745334B (zh) * 2013-09-30 2019-12-10 吴迪 通过使用邻近条形编码分析分子复合物的概况的方法
EP3080291B1 (en) * 2013-12-11 2020-02-26 Qiagen GmbH Nucleic acid detection and quantification
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170247745A1 (en) * 2014-09-12 2017-08-31 Click Diagnostics, Inc. Multiplex optical detection
LT3957753T (lt) 2014-10-10 2025-01-10 Rutgers, The State University Of New Jersey Polimerazės grandininės reakcijos pradmenys ir zondai, skirti mycobacterium tuberculosis
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
CA2971200A1 (en) 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
ES2898103T3 (es) 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
EP3748002A1 (en) * 2015-01-12 2020-12-09 Life Technologies Corporation Methods, systems and compositions thereof for nucleic acid library quality control and quantification
WO2017052300A1 (en) * 2015-09-24 2017-03-30 Seegene, Inc. . Multiple dataset analysis for determining the presence or absence of target analyte
WO2017087943A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
KR102326604B1 (ko) * 2016-06-02 2021-11-15 주식회사 씨젠 신호 변화량 데이터 세트를 이용한 샘플 내 타겟 분석물질 검출 방법
WO2018005710A1 (en) 2016-06-29 2018-01-04 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
JP6983906B2 (ja) * 2017-04-11 2021-12-17 ニューゲン テクノロジーズ, インコーポレイテッド ライブラリーの定量および定性
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
WO2019045532A2 (en) * 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
KR20200079264A (ko) 2017-11-09 2020-07-02 비스비 메디컬, 인코포레이티드 표적 바이러스 검출을 위한 휴대용 분자 진단 디바이스 및 방법
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅(武汉)生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
JP2021511056A (ja) * 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
KR102464501B1 (ko) * 2018-04-20 2022-11-17 주식회사 씨젠 샘플 내 복수의 타겟 핵산 서열의 검출을 위한 방법 및 장치
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
AU2019337088A1 (en) 2018-09-03 2021-05-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
WO2020223257A1 (en) 2019-04-28 2020-11-05 Visby Medical, Inc. Molecular diagnostic devices with digital detection capability and wireless connectivity
US20220259641A1 (en) * 2019-07-17 2022-08-18 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
EP4034674B1 (en) 2019-09-23 2025-03-26 Universiteit Gent Probe and method for str-genotyping
US11352675B2 (en) 2020-01-03 2022-06-07 Visby Medical, Inc. Devices and methods for antibiotic susceptability testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术(深圳)有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
WO2023023533A1 (en) * 2021-08-19 2023-02-23 Luminex Corporation Digital amplification assay analysis method
US12516389B2 (en) 2021-11-19 2026-01-06 Stmicroelectronics S.R.L. Microdot array having PCR-primers fixed in each microdot and method of forming the same on a substrate for gene based pathogen detection
JP7852045B2 (ja) * 2022-06-20 2026-04-27 株式会社日立ハイテク プライマー、dna検出方法およびdna検出キット
WO2026024677A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Method for determining the plasma volume of a blood sample and method for determining the dilution of plasma
WO2026024668A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Method of determining a volume of a liquid in a sample
WO2026024666A1 (en) 2024-07-22 2026-01-29 Abbott Laboratories Systems and methods for digital detection assays

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Pcr中のハイブリダイゼーションのモニタリング
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
WO2001013086A2 (en) 1999-08-13 2001-02-22 Brandeis University Detection of nucleic acids
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
DE60030145T2 (de) * 1999-10-22 2008-01-10 PHRI Properties, Inc., Newark Nachweisverfahren für kurze sequenzvarianten
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
WO2002101004A2 (en) * 2001-06-08 2002-12-19 Shanghai Mendel Dna Center Co., Ltd Low-temperature cycle extension of dna with high priming specificity
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
EP1444365A4 (en) 2001-10-25 2005-07-20 Gorilla Genomics Inc ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASERESISTENT MOLECULAR BEACONS
US20030175806A1 (en) 2001-11-21 2003-09-18 Peter Rule Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
US7659060B2 (en) * 2002-09-02 2010-02-09 Toyo Boseki Kabushiki Kaisha Method for identifying nucleotide polymorphism
AU2003297297A1 (en) * 2002-11-22 2004-06-18 North Shore-Long Island Jewish Research Institute Elf3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
ES2385485T3 (es) * 2003-12-03 2012-07-25 Abbott Laboratories Sonda de ácido nucleico lineal de doble hebra y usos de la misma
ES2425749T3 (es) * 2004-03-31 2013-10-17 The General Hospital Corporation Método para determinar la respuesta del cáncer a tratamientos dirigidos al receptor del factor de crecimiento epidérmico
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации

Also Published As

Publication number Publication date
EP1805199B1 (en) 2011-01-05
CA2855748C (en) 2018-08-28
CN101076607A (zh) 2007-11-21
KR101447405B1 (ko) 2014-10-06
KR20130021465A (ko) 2013-03-05
US20120040352A1 (en) 2012-02-16
ES2668467T3 (es) 2018-05-18
US7632642B2 (en) 2009-12-15
CA2996947A1 (en) 2006-04-27
CA2584569A1 (en) 2006-04-27
RU2460804C2 (ru) 2012-09-10
PL1805199T3 (pl) 2011-06-30
EP2927238B1 (en) 2018-01-17
CA2584569C (en) 2014-09-30
EP2351766B1 (en) 2014-12-03
PT1805199E (pt) 2011-04-11
CN101076607B (zh) 2013-04-24
WO2006044994A2 (en) 2006-04-27
KR101424812B1 (ko) 2014-08-07
KR20130113541A (ko) 2013-10-15
NO20072506L (no) 2007-07-11
KR101347951B1 (ko) 2014-01-07
EP1805199A2 (en) 2007-07-11
WO2006044994A3 (en) 2007-03-08
JP5860484B2 (ja) 2016-02-16
JP2014110793A (ja) 2014-06-19
EP2927238A1 (en) 2015-10-07
EP2351766A2 (en) 2011-08-03
CA2855748A1 (en) 2006-04-27
ES2529204T3 (es) 2015-02-17
JP5543065B2 (ja) 2014-07-09
EP2351766A3 (en) 2012-03-21
HK1106251A1 (en) 2008-03-07
EP1805199A4 (en) 2008-07-16
ES2359322T3 (es) 2011-05-20
US20130095479A1 (en) 2013-04-18
AU2005295298A1 (en) 2006-04-27
RU2007118545A (ru) 2008-11-27
CA2996947C (en) 2019-02-26
DK1805199T3 (da) 2011-04-26
JP2008516612A (ja) 2008-05-22
BRPI0517105B1 (pt) 2015-06-23
AU2005295298B2 (en) 2011-07-14
KR20070090159A (ko) 2007-09-05
US9745624B2 (en) 2017-08-29
ATE494295T1 (de) 2011-01-15
BRPI0517105A (pt) 2008-09-30
US20060177841A1 (en) 2006-08-10

Similar Documents

Publication Publication Date Title
DE602005025791D1 (de) Verfahren zur nukleinsäureamplifikation
JP2985446B2 (ja) 核酸の検出及び測定方法
CN107385040B (zh) 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
JP3785517B2 (ja) 核酸融解温度測定法
CN105821138B (zh) 一种基于连接反应构建双茎环结构dna模板检测核酸的方法
JP2008529555A (ja) 微生物のゲノムdnaを同定するデバイスおよび方法
US20150159195A1 (en) Method and device for rapid detection of amplified nucleotide sequences
DE10084146D2 (de) Verfahren zur schnellen Bestimmung von Mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
Treerattrakoon et al. Rolling circle amplification and graphene-based sensor-on-a-chip for sensitive detection of serum circulating miRNAs
JP5299986B2 (ja) 核酸の定量方法
CN111270007A (zh) 猪瘟病毒检测用引物、微流控芯片、系统及其应用
JP2002027993A (ja) 特定の核酸を検出定量するための等長プライマー伸長法およびキット
JP6374967B2 (ja) 多孔質基材における核酸増幅の検出
CN112921120B (zh) 一种可重复利用的核酸荧光探针及相应的核酸检测方法
US20130115616A1 (en) Detection of nucleic acids by agglutination
EP0877094A2 (en) Detection and quantification of one or more nucleotide sequence target analytes in a sample using spatially localized target analyte replication
JP4347404B2 (ja) 核酸の検出方法
KR101334072B1 (ko) 핵산 정량 방법 및 키트
Powell et al. Tissue source attribution using the PyroMark® Q48 Autoprep System: Sperm identification in forensic casework
WO2005001124A3 (en) Methods of nucleic acid analysis
RU2644262C1 (ru) Способ детекции множества последовательностей нуклеотидов в одном образце методом кодирования ампликонов при проведении полимеразной цепной реакции
CN114657237A (zh) 一种pcr终点可视化检测核酸的方法
KR20260044333A (ko) 물개 아넬로바이러스 4 진단용 조성물 및 이를 이용한 물개 아넬로바이러스 4 실시간 진단 방법
KR20260044336A (ko) 물개 토크 테노바이러스 5 진단용 조성물 및 이를 이용한 물개 토크 테노바이러스 5 실시간 진단 방법
KR20260044332A (ko) 물개 아넬로바이러스 2 진단용 조성물 및 이를 이용한 물개 아넬로바이러스 2 실시간 진단 방법