DD252614A5 - Verfahren zur Herstellung von Hepatitis B-Oberflächenantigenen - Google Patents

Verfahren zur Herstellung von Hepatitis B-Oberflächenantigenen Download PDF

Info

Publication number
DD252614A5
DD252614A5 DD29662586A DD29662586A DD252614A5 DD 252614 A5 DD252614 A5 DD 252614A5 DD 29662586 A DD29662586 A DD 29662586A DD 29662586 A DD29662586 A DD 29662586A DD 252614 A5 DD252614 A5 DD 252614A5
Authority
DD
German Democratic Republic
Prior art keywords
dna
plasmid
dna fragment
fragment
hepatitis
Prior art date
Application number
DD29662586A
Other languages
German (de)
English (en)
Inventor
Juerg F Tschopp
Michael M Harpold
James M Cregg
Richard G Buckholz
Original Assignee
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phillips Petroleum Co filed Critical Phillips Petroleum Co
Publication of DD252614A5 publication Critical patent/DD252614A5/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DD29662586A 1985-11-26 1986-11-25 Verfahren zur Herstellung von Hepatitis B-Oberflächenantigenen DD252614A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/801,713 US4895800A (en) 1985-11-26 1985-11-26 Yeast production of hepatitis B surface antigen

Publications (1)

Publication Number Publication Date
DD252614A5 true DD252614A5 (de) 1987-12-23

Family

ID=25181867

Family Applications (1)

Application Number Title Priority Date Filing Date
DD29662586A DD252614A5 (de) 1985-11-26 1986-11-25 Verfahren zur Herstellung von Hepatitis B-Oberflächenantigenen

Country Status (28)

Country Link
US (1) US4895800A (ja)
EP (1) EP0226846B1 (ja)
JP (1) JPH0763372B2 (ja)
KR (1) KR920004050B1 (ja)
CN (1) CN86107885A (ja)
AT (1) ATE92527T1 (ja)
AU (1) AU583683B2 (ja)
CA (1) CA1285895C (ja)
CS (1) CS276453B6 (ja)
DD (1) DD252614A5 (ja)
DE (1) DE3688831T2 (ja)
DK (1) DK565186A (ja)
EG (1) EG17949A (ja)
ES (1) ES2058055T3 (ja)
FI (1) FI95929C (ja)
HU (1) HUT43112A (ja)
IE (1) IE64519B1 (ja)
IL (1) IL80754A (ja)
IN (1) IN166428B (ja)
MX (1) MX4334A (ja)
NO (1) NO176025C (ja)
NZ (1) NZ218286A (ja)
PH (2) PH25941A (ja)
PL (1) PL152882B1 (ja)
PT (1) PT83819B (ja)
TW (1) TW215457B (ja)
YU (1) YU46031B (ja)
ZA (1) ZA868601B (ja)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4777240A (en) * 1984-03-08 1988-10-11 Scripps Clinic And Research Foundation SV40 expression vector containing HBxAg as an expression marker
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US5135868A (en) * 1985-10-25 1992-08-04 Phillips Petroleum Company Cultures of yeast of the genus Pichia altered by site selective genomic modification
US4683293A (en) * 1986-10-20 1987-07-28 Phillips Petroleum Company Purification of pichia produced lipophilic proteins
DE3882154T2 (de) * 1987-02-27 1993-12-02 Merck & Co Inc Verfahren zur Herstellung des pres 1/S2/S-Hepatitis-B-Antigens aus Hefe.
US5026828A (en) * 1987-02-27 1991-06-25 Merck & Co., Inc. Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast
EP0291146B1 (en) * 1987-03-09 1993-09-01 Merck & Co. Inc. Purification of recombinant hepatitis b surface antigen
EP0299242A3 (en) * 1987-06-22 1989-01-25 Medico Labs Ag Heterologous viral peptide particle immunogens
US5011915A (en) * 1987-10-26 1991-04-30 Merck & Co., Inc. Process for purifying recombinant hepatitis antigens
EP0314240A3 (en) * 1987-10-26 1990-03-28 Merck & Co. Inc. Process for purifying recombinant hepatitis antigens
US5004688A (en) * 1988-04-15 1991-04-02 Phillips Petroleum Company Purification of hepatitis proteins
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
IL89991A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of hepatitis b pres2 protein in methylotrophic yeasts
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL90161A0 (en) * 1988-05-13 1989-12-15 Phillips Petroleum Co Expression of hepatitis b s and pres2 proteins in methylotrophic yeasts
JPH04501662A (ja) * 1988-09-26 1992-03-26 ザ・サルク・インスティチュート・バイオテクノロジー／インダストリアル・アソシエイツ・インコーポレーテッド 混合給送組換え酵母発酵
WO1990009434A1 (en) * 1989-02-13 1990-08-23 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of superoxide dismutase in pichia pastoris yeast cells
US6197548B1 (en) 1990-04-02 2001-03-06 Medeva Pharma Limited Transformed Pichia expressing the pertactin antigen
WO1992004363A1 (en) * 1990-09-04 1992-03-19 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
CU22290A1 (es) * 1990-10-08 1995-01-31 Cigb Procedimiento para la obtencion de antigeno de superficie del virus de la hepatitis b de superior capacidad inmunoganica y su uso en un preparado vacunal
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
EP0491077A1 (en) * 1990-12-19 1992-06-24 Medeva Holdings B.V. A composition used as a therapeutic agent against chronic viral hepatic diseases
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
CA2063890C (en) * 1991-03-27 2007-03-13 Masami Miura Mutant aox2 promoter, microorganism carrying same, method of preparation thereof and production of heterologous protein using such microorganism
CA2105064A1 (en) * 1991-04-01 1992-10-02 Martin Anthony Gleeson Genes which influence pichia proteolytic activity, and uses therefor
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5850025A (en) * 1991-09-19 1998-12-15 Sibia Neurosciences, Inc. Protection of plants against plant pathogens
PT835663E (pt) 1992-05-23 2010-01-04 Glaxosmithkline Biolog Sa Vacinas combinadas compreendendo o antigénio de superfície da hepatite b e outros antigénios
PT761231E (pt) 1992-06-25 2000-06-30 Smithkline Beecham Biolog Composicao de vacina contendo adjuvantes
JPH06209763A (ja) * 1993-01-13 1994-08-02 Green Cross Corp:The 変異株
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
NZ296648A (en) * 1994-10-18 2001-05-25 Corvas Int Inc Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5955349A (en) * 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5716808A (en) * 1995-11-09 1998-02-10 Zymogenetics, Inc. Genetic engineering of pichia methanolica
GB9623233D0 (en) 1996-11-07 1997-01-08 Smithkline Beecham Biolog Vaccine composition
DE69935606T9 (de) 1998-10-16 2021-03-11 Glaxosmithkline Biologicals S.A. Adjuvanzsysteme und impfstoffe
UA79735C2 (uk) 2000-08-10 2007-07-25 Глаксосмітклайн Байолоджікалз С.А. Очищення антигенів вірусу гепатиту b (hbv) для використання у вакцинах
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
DE60319333D1 (de) * 2002-12-20 2008-04-10 Roche Diagnostics Gmbh Hitzelabile Desoxyribonuklease I-Varianten
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
JP4411192B2 (ja) * 2003-12-05 2010-02-10 エフ．ホフマン−ラ ロシュ アーゲー 組換えで発現されるカルボキシペプチダーゼｂおよびその精製
MX2008003362A (es) * 2005-09-14 2008-03-25 Sanofi Aventis Deutschland Escision de precursores de insulinas mediante una variante de tripsina.
EP2441469A1 (en) 2006-03-14 2012-04-18 Oregon Health and Science University Methods for producing an immune response to tuberculosis
AU2007293672B2 (en) 2006-09-07 2013-06-27 Glaxosmithkline Biologicals S.A. Vaccine
JP4216875B2 (ja) * 2006-09-29 2009-01-28 株式会社東芝 メタノール応答性プロモーター、プロモーターと外来遺伝子を発現可能な様式で連結した融合遺伝子、ベクター、形質転換体、およびタンパク質の生産方法
EP2444410A3 (en) 2007-02-28 2012-08-08 The Govt. Of U.S.A. As Represented By The Secretary Of The Department Of Health And Human Services Brachyury polypeptides and methods for use
KR20100017569A (ko) 2007-05-02 2010-02-16 글락소스미스클라인 바이오로지칼즈 에스.에이. 백신
AU2009248810B2 (en) 2008-05-23 2013-12-05 The Regents Of The University Of Michigan Nanoemulsion vaccines
US9005631B2 (en) 2008-08-04 2015-04-14 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Membrane proximal region of HIV gp41 anchored to the lipid layer of a virus-like particle vaccine
US8664183B2 (en) 2009-02-27 2014-03-04 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services SPANX-B polypeptides and their use
WO2011047340A1 (en) 2009-10-16 2011-04-21 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Insertion of foreign genes in rubella virus and their stable expression in a live, attenuated viral vaccine
GB201105981D0 (en) 2011-04-08 2011-05-18 Glaxosmithkline Biolog Sa Novel process
US9566329B2 (en) 2012-04-06 2017-02-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Live, attenuated rubella vector to express vaccine antigens
EP2895191B1 (en) 2012-09-14 2019-06-05 The U.S.A. as represented by the Secretary, Department of Health and Human Services Brachyury protein, adenoviral vectors encoding brachyury protein, and their use
MY172181A (en) 2013-03-08 2019-11-15 Janssen Vaccines & Prevention Bv Acellular pertussis vaccine
EP3331554B1 (en) 2015-08-03 2022-05-11 The U.S.A. as represented by the Secretary, Department of Health and Human Services Brachyury deletion mutants, non-yeast vectors encoding brachyury deletion mutants, and their use

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2480779B2 (fr) * 1979-08-30 1986-07-18 Anvar Vecteur contenant une sequence nucleotidique de l'antigene de surface du virus de l'hepatite b et procede de fabrication d'une molecule immunogene mettant en oeuvre ce vecteur
GB2055847A (en) * 1979-07-30 1981-03-11 Bull F G Process for the production of carrier particles
US4769238A (en) * 1981-08-04 1988-09-06 The Regents Of The University Of California Synthesis of human virus antigens by yeast
US4803164A (en) * 1981-08-31 1989-02-07 Genentech, Inc. Preparation of hepatitis b surface antigen in yeast
NZ201705A (en) * 1981-08-31 1986-03-14 Genentech Inc Recombinant dna method for production of hepatitis b surface antigen in yeast
DE3381566D1 (de) * 1982-08-16 1990-06-21 Agency Science & Tech Hepatitis-b-virus-gen enthaltendes rekombinantes plasmid, mit diesem rekombinanten plasmid transformierte hefe und herstellung von hepatitis-b-virus-oberflaechenantigen.
IL69202A (en) * 1982-09-08 1991-08-16 Smith Kline Rit Surface antigen polypeptides of hbv virus produced in yeast and the preparation thereof
US4510245A (en) * 1982-11-18 1985-04-09 Chiron Corporation Adenovirus promoter system
CA1341302C (en) * 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
US4515714A (en) * 1983-03-09 1985-05-07 Juridicial Foundation, The Chemo-Semo-Sero-Therapeutic Research Institute Method for purification of hepatitis B virus surface antigen
KR850001534A (ko) * 1983-08-22 1985-03-30 제임스 에프. 나우톤 형질전환된 효모로 부터 유도된 면역원성 HBsAg
US4614793A (en) * 1983-10-14 1986-09-30 Merck & Co., Inc. Hepatitis A--subunit antigen
JPS60196185A (ja) * 1984-03-19 1985-10-04 Chemo Sero Therapeut Res Inst 形質転換酵母の培養方法
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia

Also Published As

Publication number Publication date
IE64519B1 (en) 1995-08-09
DE3688831D1 (de) 1993-09-09
FI864791A0 (fi) 1986-11-25
PH25941A (en) 1991-12-19
NO176025C (no) 1995-01-18
CS864186A3 (en) 1992-01-15
YU201386A (en) 1988-10-31
ES2058055T3 (es) 1994-11-01
ATE92527T1 (de) 1993-08-15
IL80754A (en) 1993-03-15
EP0226846B1 (en) 1993-08-04
AU6538486A (en) 1987-05-28
PL152882B1 (en) 1991-02-28
DE3688831T2 (de) 1993-11-25
IN166428B (ja) 1990-05-05
NO176025B (no) 1994-10-10
ZA868601B (en) 1987-06-24
CA1285895C (en) 1991-07-09
JPH0763372B2 (ja) 1995-07-12
KR920004050B1 (ko) 1992-05-23
EP0226846A1 (en) 1987-07-01
CS276453B6 (en) 1992-06-17
YU46031B (sh) 1992-12-21
TW215457B (ja) 1993-11-01
PH25539A (en) 1991-07-24
MX4334A (es) 1993-12-01
NO864704D0 (no) 1986-11-25
FI95929B (fi) 1995-12-29
CN86107885A (zh) 1987-09-23
AU583683B2 (en) 1989-05-04
DK565186D0 (da) 1986-11-25
PT83819B (pt) 1988-10-14
DK565186A (da) 1987-05-27
KR870005099A (ko) 1987-06-04
FI95929C (fi) 1996-04-10
EG17949A (en) 1991-03-30
FI864791A (fi) 1987-05-27
PT83819A (en) 1986-12-01
HUT43112A (en) 1987-09-28
US4895800A (en) 1990-01-23
IL80754A0 (en) 1987-02-27
PL262598A1 (en) 1987-09-21
NZ218286A (en) 1991-07-26
IE863021L (en) 1987-05-26
JPS62190085A (ja) 1987-08-20

Similar Documents

Publication Publication Date Title
DD252614A5 (de) Verfahren zur Herstellung von Hepatitis B-Oberflächenantigenen
US4855231A (en) Regulatory region for heterologous gene expression in yeast
DD255544A5 (de) Verfahren zur ortsselektiven Genommodifikation von Hefen der Gattung Pichia
DD284899A5 (de) Verfahren zur herstellung eines plasmodium-impfstoffes
DD218118A5 (de) Verfahren zur herstellung eines hefe- oder nichthefepolypeptids
JP2580036B2 (ja) メチロトローフ酵母におけるヒト血清アルブミンの発現
CA1340617C (en) Expression of hepatitis b s and pres2 protiens in methylotrophic yeasts
JPH04117291A (ja) ヘテロＤＮＡ配列およびその配列を用いた工業用酵母ヤロウイア属（Ｙａｒｒｏｗｉａ）の形質転換
DD284047A5 (de) Verfahren zur herstellung einer dna-sequenz, die fuer einen alkohol-oxidose-ii-regulationsbereich einer methylotrophen hefe kodiert
DE3308030A1 (de) Tierische interferone
DE69133525T2 (de) Verfahren zur Herstellung von recombinantem Hepatitis B Oberflächenantigen mit erhöhter Immunogen-Kapazität und Verwendung in einer Impfstoffzubereitung
DD283836A5 (de) Verfahren zur bildung von antigenen hbv-teilchen
EP0339568A1 (en) Expression of Human Interleukin-2 in Methylotrophic Yeasts
DE69433222T2 (de) Mutanter AOX2-Promotor
EP0503252B1 (de) Rekombinante Proteine mit der Immunreaktivität des Hepatitis B Virus e Antigens (HBeAg), Verfahren zu ihrer Herstellung und ihre Verwendung in Immunoassays und Impfstoffen
DD257646A5 (de) Verfahren zur Herstellung von Streptokinase
EP0248359A1 (de) Verfahren zur Sekretion von Proteinen aus Hefen
EP0149430A1 (de) Verfahren zur Herstellung chimärer DNS
GB2217332A (en) Expression of salmon growth hormone in methylotrophic yeast of genus pichia
DD240029A1 (de) Verfahren zur herstellung eines blv-kodierten huellproteins
EP0431543A1 (de) Verfahren zur Expression von Fremdgenen in Hefen
DD280119A1 (de) Verfahren zur herstellung von hbv-dna-rekombinantenklonen
DD274052A5 (de) Verfahren zur Herstellung von Rekombinanten Hbv-, Hiv- oder Plasmodium-Oberflächenproteinen bzw. Hybridpartikeln
DD226592A1 (de) Verfahren zur gewinnung von isogenen streptomyces-plasmiden

Legal Events

Date Code Title Description
RPI Change in the person, name or address of the patentee (searches according to art. 11 and 12 extension act)
RPV Change in the person, the name or the address of the representative (searches according to art. 11 and 12 extension act)
ASS Change of applicant or owner

Owner name: RESEARCH CORPORATION TECHNOLOGIES, INC

Effective date: 19940503

IF04 In force in the year 2004

Expiry date: 20061126