CZ293278B6 - Způsob přípravy komplexních DNA methylačních peptidových map - Google Patents

Způsob přípravy komplexních DNA methylačních peptidových map Download PDF

Info

Publication number
CZ293278B6
CZ293278B6 CZ20001934A CZ20001934A CZ293278B6 CZ 293278 B6 CZ293278 B6 CZ 293278B6 CZ 20001934 A CZ20001934 A CZ 20001934A CZ 20001934 A CZ20001934 A CZ 20001934A CZ 293278 B6 CZ293278 B6 CZ 293278B6
Authority
CZ
Czechia
Prior art keywords
dna
oligonucleotides
reaction
bisulfite
sequence
Prior art date
Application number
CZ20001934A
Other languages
Czech (cs)
English (en)
Other versions
CZ20001934A3 (cs
Inventor
Alexander Olek
Sven Stefan Olek
Jörn WALTER
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Publication of CZ20001934A3 publication Critical patent/CZ20001934A3/cs
Publication of CZ293278B6 publication Critical patent/CZ293278B6/cs

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/112Disease subtyping, staging or classification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CZ20001934A 1997-11-27 1998-11-27 Způsob přípravy komplexních DNA methylačních peptidových map CZ293278B6 (cs)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19754482A DE19754482A1 (de) 1997-11-27 1997-11-27 Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke

Publications (2)

Publication Number Publication Date
CZ20001934A3 CZ20001934A3 (cs) 2000-11-15
CZ293278B6 true CZ293278B6 (cs) 2004-03-17

Family

ID=7851173

Family Applications (1)

Application Number Title Priority Date Filing Date
CZ20001934A CZ293278B6 (cs) 1997-11-27 1998-11-27 Způsob přípravy komplexních DNA methylačních peptidových map

Country Status (19)

Country Link
US (1) US6214556B1 (https=)
EP (1) EP1034309B1 (https=)
JP (1) JP2001525181A (https=)
CN (1) CN1253582C (https=)
AT (1) ATE217348T1 (https=)
AU (1) AU753368B2 (https=)
CA (1) CA2310384C (https=)
CZ (1) CZ293278B6 (https=)
DE (2) DE19754482A1 (https=)
DK (1) DK1034309T3 (https=)
ES (1) ES2173669T3 (https=)
HU (1) HUP0100424A3 (https=)
IL (1) IL136158A (https=)
IS (1) IS1921B (https=)
NZ (1) NZ504586A (https=)
PL (1) PL341681A1 (https=)
PT (1) PT1034309E (https=)
RU (1) RU2223324C2 (https=)
WO (1) WO1999028498A2 (https=)

Families Citing this family (210)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6979728B2 (en) * 1998-05-04 2005-12-27 Baylor College Of Medicine Articles of manufacture and methods for array based analysis of biological molecules
DE19853398C1 (de) * 1998-11-19 2000-03-16 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischer DNA
US7058517B1 (en) 1999-06-25 2006-06-06 Genaissance Pharmaceuticals, Inc. Methods for obtaining and using haplotype data
AU5638600A (en) * 1999-06-25 2001-01-31 Genaissance Pharmaceuticals, Inc. Methods for obtaining and using haplotype data
DE19935772C2 (de) * 1999-07-26 2002-11-07 Epigenomics Ag Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben
DE19935749C2 (de) * 1999-07-28 2003-06-26 Epigenomics Ag Verfahren zur Chrakterisierung von Nukleinsäurefragmenten
CA2387035A1 (en) * 1999-10-13 2001-04-19 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
US7668658B2 (en) 1999-10-13 2010-02-23 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
DE19951189C2 (de) * 1999-10-15 2003-11-06 Epigenomics Ag Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA
WO2001036669A2 (de) * 1999-11-12 2001-05-25 Epigenomics Ag Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen
DE19957827C2 (de) * 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
DE19959691A1 (de) * 1999-12-06 2001-08-16 Epigenomics Ag Verfahren zur parallelen Detektions des Methylierungszustandes von genomischer DNA
WO2001044634A1 (en) * 1999-12-15 2001-06-21 Komatsu Zenoah Co. Piston valve type layered scavenging 2-cycle engine
GB9929720D0 (en) * 1999-12-17 2000-02-09 Zeneca Ltd Diagnostic method
DE19963536C2 (de) * 1999-12-20 2003-04-10 Epigenomics Ag Verfahren zur Analyse von Nukleinsäuresequenzen
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8076063B2 (en) * 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
DE10010280B4 (de) * 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010281B4 (de) * 2000-02-25 2005-03-10 Epigenomics Ag Ligase/Polymerase-Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
US20040048254A1 (en) * 2000-03-15 2004-03-11 Alexander Olek Diagnosis of diseases associated with tumor supressor genes and oncogenes
DE10013847A1 (de) * 2000-03-15 2001-09-27 Epigenomics Ag Oligonukleotide oder PNA-Oligomere und Verfahren zur parallelen Detektion des Methylierungszustandes genomischer DNA
DE10019058A1 (de) * 2000-04-06 2001-12-20 Epigenomics Ag Detektion von Variationen des DNA-Methylierungsprofils
DE10032529A1 (de) * 2000-06-30 2002-02-07 Epigenomics Ag Diagnose von bedeutenden genetischen Parametern innerhalb des Major Histocompatibility Complex (MHC)
WO2001077375A2 (en) * 2000-04-06 2001-10-18 Epigenomics Ag Diagnosis of diseases associated with gene regulation
EP1268856A2 (de) * 2000-04-07 2003-01-02 Epigenomics AG Detektion von snp's und cytosin-methylierungen
DE10347399B4 (de) * 2003-10-09 2005-09-15 Epigenomics Ag Bisulfit-Umwandlung zum Nachweis von Cytosin-Methylierungen in DNA mittels optimierter Aufreinigung
DE10029914A1 (de) * 2000-06-19 2002-01-03 Epigenomics Ag Verfahren zur hochparallelen Analyse von Polymorphismen
DE10347396B4 (de) * 2003-10-09 2005-06-23 Epigenomics Ag Optimierte Bisulfit-Umwandlung von DNA durch Einsatz von Dioxan
US20060134643A1 (en) 2000-06-19 2006-06-22 Kurt Berlin Bisulfite conversion of DNA
DE10347400B4 (de) * 2003-10-09 2005-08-04 Epigenomics Ag Verbesserte Bisulfit-Umwandlung von DNA durch kurzzeitige Temperaturerhöhungen
DE10347397B4 (de) * 2003-10-09 2005-08-04 Epigenomics Ag Optimierte Bisulfit-Umwandlung durch Zusatz von n-Alkylenglykol-Verbindungen
DE10029915B4 (de) * 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
US6931326B1 (en) 2000-06-26 2005-08-16 Genaissance Pharmaceuticals, Inc. Methods for obtaining and using haplotype data
WO2002002807A2 (en) * 2000-06-30 2002-01-10 Epigenomics Ag Diagnosis of diseases associated with cell signalling
WO2002018631A2 (de) * 2000-09-01 2002-03-07 Epigenomics Ag Diagnose von bestehenden erkrankungen oder der prädisposition für bestimmte erkrankungen
IL154663A0 (en) * 2000-09-01 2003-09-17 Epigenomics Ag Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3'
DE10044543C2 (de) * 2000-09-05 2003-09-11 Epigenomics Ag Verfahren zur Bestimmung des Methylierungsgrades von bestimmten Cytosinen in genomischer DNA im Sequenzkontext 5'-CpG-3'
DE10050942B4 (de) * 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
US6868342B2 (en) 2000-10-13 2005-03-15 The Brigham & Women's Hospital, Inc. Method and display for multivariate classification
DE10054974A1 (de) * 2000-11-06 2002-06-06 Epigenomics Ag Diagnose von mit Cdk4 assoziierten Krankheiten
AUPR142500A0 (en) * 2000-11-13 2000-12-07 Human Genetic Signatures Pty Ltd A peptide nucleic acid-based assay for the detection of specific nucleic acid sequences
DE10056802B4 (de) * 2000-11-14 2005-06-16 Epigenomics Ag Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik
DE10061348C2 (de) * 2000-12-06 2002-10-24 Epigenomics Ag Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA
DE10061338A1 (de) * 2000-12-06 2002-06-20 Epigenomics Ag Diagnose von mit Angiogenese assoziierten Krankheiten
JP2002171973A (ja) * 2000-12-07 2002-06-18 Univ Tokyo Dnaメチル化パターンによる細胞の同定法
DE10065814B4 (de) * 2000-12-22 2004-07-08 Epigenomics Ag Verfahren für die simultane Amplifikation vieler Sequenzen in einer PCR-Reaktion und deren Markierung
DE10065815A1 (de) * 2000-12-22 2002-07-11 Epigenomics Ag Verfahren zur flexiblen Herstellung von Oligomer-Arrays
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
DE10104937B4 (de) * 2001-01-29 2005-03-17 Epigenomics Ag Fluoreszenzpolarisation 2
US6893820B1 (en) * 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
WO2002070741A2 (en) * 2001-03-01 2002-09-12 Epigenomics Ag Methods, systems and computer program products for determining the biological effect and/or activity of drugs, chemical substances and/or pharmaceutical compositions based on their effect on the methylation status of the dna
EP1627927A3 (en) * 2001-03-01 2006-06-07 Epigenomics AG Methods, systems and computer program products for determining the biological effect and/or activity of drugs, chemical substances and/or pharmaceutical compositions based on their effect on the methylation status of the DNA
EP1410304A2 (en) * 2001-03-26 2004-04-21 Epigenomics AG Method for epigenetic feature selection
DE10119468A1 (de) * 2001-04-12 2002-10-24 Epigenomics Ag Mikroarray-Verfahren zur Anreicherung von DNA-Fragmenten aus komplexen Mischungen
DE10128509A1 (de) * 2001-06-14 2003-01-02 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata- und Nierenkarzinomen
DE10128508A1 (de) * 2001-06-14 2003-02-06 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren
DE10130800B4 (de) * 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
DE10132212B4 (de) * 2001-06-27 2005-11-24 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung durch vergleichende Analyse der Einzelstränge von Amplifikaten
WO2003004696A2 (en) * 2001-07-02 2003-01-16 Epigenomics Ag A distributed system for epigenetic based prediction of complex phenotypes
WO2003012051A2 (en) * 2001-07-31 2003-02-13 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Inhibitor of dna methylation
DE10139283A1 (de) * 2001-08-09 2003-03-13 Epigenomics Ag Verfahren und Nukleinsäuren zur Analyse von Colon-Krebs
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US20050032060A1 (en) * 2001-08-31 2005-02-10 Shishir Shah Arrays comprising pre-labeled biological molecules and methods for making and using these arrays
DE10151055B4 (de) * 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
WO2003038726A2 (en) * 2001-10-31 2003-05-08 Epigenomics Ag A method for epigenetic knowledge generation
JP2003144172A (ja) * 2001-11-16 2003-05-20 Nisshinbo Ind Inc メチル化検出用オリゴヌクレオチド固定化基板
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
DE10161625A1 (de) * 2001-12-14 2003-07-10 Epigenomics Ag Verfahren und Nukleinsäuren für die Analyse einer Lungenzell-Zellteilungsstörung
US20040267458A1 (en) * 2001-12-21 2004-12-30 Judson Richard S. Methods for obtaining and using haplotype data
DE10201138B4 (de) * 2002-01-08 2005-03-10 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)
WO2003064701A2 (en) * 2002-01-30 2003-08-07 Epigenomics Ag Method for the analysis of cytosine methylation patterns
DE10204566A1 (de) * 2002-02-04 2003-08-14 Nanogen Recognomics Gmbh Verfahren zur Bestimmung des Methylierungsmusters von DNA
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
EP1340818A1 (en) 2002-02-27 2003-09-03 Epigenomics AG Method and nucleic acids for the analysis of a colon cell proliferative disorder
DK1342794T3 (da) * 2002-03-05 2006-04-24 Epigenomics Ag Fremgangsmåde og anordning til at bestemme vævsspecificitet af fritflydende DNA i legemsvæsker
EP1344832A1 (en) * 2002-03-15 2003-09-17 Epigenomics AG Methods and nucleic acids for the analysis of methylation within the gene melastatin
EP1488003A1 (en) * 2002-03-25 2004-12-22 Epigenomics AG Method for the analysis of methylation patterns within nucleic acids by means of mass spectrometry
US6916621B2 (en) * 2002-03-27 2005-07-12 Spectral Genomics, Inc. Methods for array-based comparitive binding assays
WO2003085132A2 (en) * 2002-04-09 2003-10-16 Epigenomics Ag Method for analysis of methylated nucleic acids
US9943847B2 (en) 2002-04-17 2018-04-17 Cytonome/St, Llc Microfluidic system including a bubble valve for regulating fluid flow through a microchannel
US6808075B2 (en) 2002-04-17 2004-10-26 Cytonome, Inc. Method and apparatus for sorting particles
US7157274B2 (en) * 2002-06-24 2007-01-02 Cytonome, Inc. Method and apparatus for sorting particles
US20070065808A1 (en) * 2002-04-17 2007-03-22 Cytonome, Inc. Method and apparatus for sorting particles
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
DE10236406C1 (de) 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
WO2004013284A2 (en) * 2002-08-02 2004-02-12 Tufts University A method to assess genomic dna methylation using high-performance liquid chromatography-electospray ionization mass spectrometry
US6931688B2 (en) * 2002-08-09 2005-08-23 Colgate-Palmolive Company Toothbrush
EP1554407B1 (en) 2002-10-01 2009-07-29 Epigenomics AG Method for the treatment of breast cell proliferative disorders
US20040101843A1 (en) * 2002-11-22 2004-05-27 Gerald Zon Detection of methylated DNA sites
AU2003298733B2 (en) * 2002-11-27 2009-06-18 Agena Bioscience, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
EP1567669B1 (en) * 2002-12-02 2010-03-24 Illumina Cambridge Limited Determination of methylation of nucleic acid sequences
EP1428889A1 (en) * 2002-12-10 2004-06-16 Epigenomics AG Method for monitoring the transition of a cell from one state into another
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1618216A2 (en) * 2003-04-25 2006-01-25 Sequenom, Inc. Fragmentation-based methods and systems for de novo sequencing
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
EP1641936B1 (en) * 2003-06-17 2010-08-04 Human Genetic Signatures PTY Ltd. Methods for genome amplification
US8163488B2 (en) 2003-06-23 2012-04-24 Epigenomics Ag Methods and nucleic acids for analysis of colon proliferative disorders
AU2004252554B2 (en) 2003-06-23 2012-01-19 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
WO2005001141A2 (en) 2003-06-23 2005-01-06 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
US20060183128A1 (en) * 2003-08-12 2006-08-17 Epigenomics Ag Methods and compositions for differentiating tissues for cell types using epigenetic markers
US7371526B2 (en) * 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
WO2005024068A2 (en) * 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
CA2540310A1 (en) * 2003-10-09 2005-04-28 Epigenomics Ag Improved bisulfite conversion of dna
ES2801379T3 (es) 2003-12-01 2021-01-11 Epigenomics Ag Métodos y ácidos nucleicos para el análisis de la expresión génica asociada con el desarrollo de trastornos proliferativos de células de próstata
CA2549852A1 (en) 2003-12-11 2005-06-30 Epigenomics Ag Method and nucleic acids for the improved treatment of breast cell proliferative disorders
CA2555704A1 (en) * 2004-02-10 2005-08-25 Cornell Research Foundation, Inc. Method for detection of promoter methylation status
EP1568786A3 (en) * 2004-02-13 2007-08-29 Affymetrix, Inc. (A US Entity) Analysis of methylation status using nucleic acid arrays
EP2380993B1 (en) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
CA2561381C (en) 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US7279281B2 (en) * 2004-06-01 2007-10-09 The Regents Of The University Of Michigan Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases
WO2006002344A1 (en) 2004-06-23 2006-01-05 Epigenomics Ag Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders
US20090298054A1 (en) 2004-07-18 2009-12-03 Epigenomics Ag Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
JP2008512129A (ja) * 2004-09-10 2008-04-24 セクエノム,インコーポレイティド 核酸の広範囲配列分析法
ES2787454T3 (es) 2004-09-30 2020-10-16 Epigenomics Ag Método para proveer fragmentos de ADN derivados de una muestra archivada
US7658288B2 (en) * 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP2311984A1 (en) 2004-12-02 2011-04-20 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders
US9260693B2 (en) 2004-12-03 2016-02-16 Cytonome/St, Llc Actuation of parallel microfluidic arrays
US7833942B2 (en) * 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
WO2006088940A2 (en) 2005-02-14 2006-08-24 The Johns Hopkins University Neoplasia screening compositions and methods of use
EP1748080A3 (en) 2005-03-11 2007-04-11 Epiontis GmbH Specific DNAs for epigenetic characterisation of cells and tissues
US8298762B2 (en) 2005-03-11 2012-10-30 Epiontis Gmbh Specific DNAS for Epigenetic Characterisation of cells and tissues
WO2006113770A1 (en) 2005-04-15 2006-10-26 Epigenomics Ag A method for providing dna fragments derived from a remote sample
EA013634B1 (ru) 2005-04-15 2010-06-30 Эпиджиномикс Аг Способы и нуклеиновые кислоты для анализов клеточных пролиферативных нарушений
EP2386654A1 (en) 2005-05-02 2011-11-16 University of Southern California DNA Methylation markers associated with the CpG island methylator phenotype (cimp) in human colorectal cancer
US7439024B2 (en) * 2005-06-01 2008-10-21 The United States Of America As Represented By The Department Of Veterans Affairs Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20090005268A1 (en) * 2005-07-18 2009-01-01 Epigenomics Ag Compositions and Methods for Cancer Diagnostics Comprising Pan-Cancer Markers
WO2007018602A1 (en) * 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
EP2298932A1 (en) 2005-09-29 2011-03-23 Epigenomics AG Methods and nucleic acids for the analysis of gene expression, in particular methylation of KAAG1, associated with tissue classification
US8912129B2 (en) 2005-11-17 2014-12-16 Epigenomics Ag Method for the determination of the DNA methylation level of a CPG position in identical cells within a tissue sample
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
CA2649777C (en) 2006-04-17 2018-08-14 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
EP2044221B1 (en) 2006-07-21 2015-07-22 Epigenomics AG Methods and kit for analyses of methylation in colorectal cellular proliferative disorders
CA2660218A1 (en) * 2006-08-08 2008-02-14 Epigenomics Ag A method for methylation analysis of nucleic acid
EP2087137B1 (en) 2006-10-18 2011-11-30 Epigenomics AG A molecule for providing a standard for the quantitative analysis of the methylation status of a nucleic acid
EP3184649A1 (en) 2006-11-24 2017-06-28 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Dnaメチル化検出における非メチル化シトシン変換処理後のdna量の確認方法
JP2010514692A (ja) 2006-12-20 2010-05-06 バイエル ヘルスケア リミティド ライアビリティ カンパニー 癌の治療に有用なヒドロキシメチルフェニルピラゾリル尿素化合物
WO2008087040A2 (en) 2007-01-19 2008-07-24 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
US8623599B2 (en) 2007-06-08 2014-01-07 Epigenomics Ag Method for methylation analysis
US20090042290A1 (en) * 2007-08-06 2009-02-12 Christopher Steele Method of modifying a macromolecule without prior extraction from a sample
CA2708163A1 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
EP2271772B1 (en) * 2008-03-11 2014-07-16 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
ES2606703T3 (es) * 2008-07-15 2017-03-27 Epigenomics Ag Método para predecir el pronóstico de una terapia de cáncer de mama con base en el análisis de metilación del gen
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
EP2966181B1 (en) 2009-06-26 2018-02-14 Epigenomics AG Methods and nucleic acids for analysis of bladder carcinoma
WO2011008541A2 (en) * 2009-06-29 2011-01-20 The Regents Of The University Of California Molecular markers and assay methods for characterizing cells
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
AU2012249531B2 (en) 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
WO2012162139A1 (en) 2011-05-20 2012-11-29 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
NO2729579T3 (https=) 2011-07-08 2018-03-03
EP2820129A1 (en) 2012-03-02 2015-01-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CN105378108A (zh) 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
EP2971171A4 (en) 2013-03-14 2016-11-02 Abbott Molecular Inc SYSTEMS AND METHODS FOR MULTIPLEX AMPLIFICATION SPECIFIC TO METHYLATION
CA2902916C (en) 2013-03-14 2018-08-28 Mayo Foundation For Medical Education And Research Detecting neoplasm
US10337049B2 (en) 2013-06-17 2019-07-02 The Trustees Of Columbia University In The City Of New York Universal methylation profiling methods
WO2015124921A1 (en) 2014-02-19 2015-08-27 The University Court Of The University Of Edinburgh Methods and uses for determining the presence of inflammatory bowel disease
EP3736344A1 (en) 2014-03-13 2020-11-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN120700143A (zh) 2014-03-31 2025-09-26 梅奥医学教育和研究基金会 检测结直肠赘生物
US10174383B2 (en) * 2014-08-13 2019-01-08 Vanadis Diagnostics Method of estimating the amount of a methylated locus in a sample
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
US10957421B2 (en) 2014-12-03 2021-03-23 Syracuse University System and method for inter-species DNA mixture interpretation
CA3183545C (en) 2014-12-12 2026-01-13 Exact Sciences Corporation Compositions and methods for performing methylation detection assays
US10435755B2 (en) 2015-03-27 2019-10-08 Exact Sciences Development Company, Llc Detecting esophageal disorders
CN114574585A (zh) 2015-08-31 2022-06-03 梅约医药教育及研究基金会 检测胃肿瘤
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
CN109563546B (zh) 2016-05-05 2022-09-09 精密科学发展有限责任公司 通过分析甲基化dna来检测肺肿瘤
EP3978624B1 (en) 2016-07-19 2025-03-26 Exact Sciences Corporation Methylated control dna
EP3507298B1 (en) 2016-09-02 2023-08-30 Mayo Foundation for Medical Education and Research Detecting hepatocellular carcinoma
KR102892245B1 (ko) 2017-01-27 2025-11-27 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 결장 신조직형성의 검출
JP7356349B2 (ja) 2017-02-28 2023-10-04 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 前立腺癌検出
CN111032868A (zh) * 2017-06-30 2020-04-17 加利福尼亚大学董事会 用于评估无细胞dna中的dna甲基化的方法和系统
JP7277460B2 (ja) 2017-11-30 2023-05-19 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 乳癌の検出
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
EP3740589A4 (en) 2018-01-17 2021-11-03 The Regents of the University of California BIOMARKERS BASED ON DNA METHYLATION AND PHENOTYPIC AGE FOR LIFE EXPECTANCY AND MORBIDITY
WO2020076983A1 (en) 2018-10-10 2020-04-16 The Regents Of The University Of California Dna methylation based biomarkers for life expectancy and morbidity
EP3880847B1 (en) 2018-11-16 2025-09-03 Oslo Universitetssykehus HF Methods and compositions for characterizing bladder cancer
JP7320067B2 (ja) 2019-01-18 2023-08-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 保存された遺伝子座に基づく、哺乳動物に関するdnaメチル化測定
CA3127329A1 (en) 2019-01-24 2020-07-30 Mayo Foundation For Medical Education And Research Detecting endometrial cancer
US11702704B2 (en) 2019-10-31 2023-07-18 Mayo Foundation For Medical Education And Research Detecting ovarian cancer
AU2021329899A1 (en) 2020-08-19 2023-03-09 Exact Sciences Corporation Detecting non-hodgkin lymphoma
KR20230150814A (ko) 2021-01-29 2023-10-31 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 다양한 유형의 암에 대한 존재 또는 부재의 검출
US20250210133A1 (en) 2022-03-15 2025-06-26 Genknowme S.A. Method Determining the Difference Between the Biological Age and the Chronological Age of a Subject
EP4728518A1 (en) 2023-06-15 2026-04-22 Genknowme S.A. Computer implemented method determining a value of allostatic load of a human being

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0734436B1 (en) * 1993-11-30 1999-04-14 McGILL UNIVERSITY Inhibition of dna methyltransferase
US5871917A (en) * 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids

Also Published As

Publication number Publication date
IS5493A (is) 2000-05-12
DK1034309T3 (da) 2002-08-26
JP2001525181A (ja) 2001-12-11
ES2173669T3 (es) 2002-10-16
RU2223324C2 (ru) 2004-02-10
US6214556B1 (en) 2001-04-10
EP1034309A2 (de) 2000-09-13
CZ20001934A3 (cs) 2000-11-15
WO1999028498A2 (de) 1999-06-10
WO1999028498A3 (de) 1999-09-02
IL136158A (en) 2004-07-25
CA2310384C (en) 2007-05-22
IS1921B (is) 2004-03-15
AU2408599A (en) 1999-06-16
EP1034309B1 (de) 2002-05-08
CN1283235A (zh) 2001-02-07
HUP0100424A3 (en) 2003-08-28
NZ504586A (en) 2002-10-25
CN1253582C (zh) 2006-04-26
DE19754482A1 (de) 1999-07-01
AU753368B2 (en) 2002-10-17
ATE217348T1 (de) 2002-05-15
HK1033473A1 (en) 2001-08-31
CA2310384A1 (en) 1999-06-10
PT1034309E (pt) 2002-10-31
PL341681A1 (en) 2001-04-23
DE59804090D1 (de) 2002-06-13
IL136158A0 (en) 2001-05-20
HUP0100424A2 (hu) 2001-06-28

Similar Documents

Publication Publication Date Title
CZ293278B6 (cs) Způsob přípravy komplexních DNA methylačních peptidových map
US20250109439A1 (en) Methods for determining fraction of fetal nucleic acids in maternal samples
EP1759011B1 (en) Detection of chromosomal disorders
AU2010343276B2 (en) Methods for determining fraction of fetal nucleic acid in maternal samples
KR20070011354A (ko) 취약 x염색체 증후군과 같은 strp의 검출 방법
WO2013103945A1 (en) Composite assay for developmental disorders
BRPI0616323A2 (pt) mÉtodo para determinar se um fluido mamÁrio doado foi obtido a partir de um indivÍduo especÍfico
WO2018147438A1 (ja) Hla遺伝子のpcrプライマーセット及びそれを用いたシークエンス法
JP2016516449A (ja) Hlaマーカーを使用する母体血液中の胎児dna分率の決定方法
US20200040390A1 (en) Methods for Sequencing Repetitive Genomic Regions
US11535897B2 (en) Composite epigenetic biomarkers for accurate screening, diagnosis and prognosis of colorectal cancer
CN115851973A (zh) 实时荧光PCR快速检测人类InDel遗传多态性的方法、试剂盒和应用
US20060099581A1 (en) Method for analysis of methylated nucleic acids
EP1951894A1 (en) Microarrays for genotyping and methods of use
Deharvengt et al. Molecular assessment of human diseases in the clinical laboratory
RU2738752C1 (ru) Способ идентификации личности и установления родства с помощью InDel полиморфизмов и набор синтетических олигонуклеотидов для их генотипирования
KR102816628B1 (ko) 대사증후군 특이적 후성유전 메틸화 마커 및 이의 용도
MXPA00004986A (en) Method for producing complex dna methylation fingerprints
CN118234874A (zh) 一种基于标签序列和链置换的全基因组甲基建库测序方法
HK1033473B (en) Method for producing complex dna methylation fingerprints
JPH09285300A (ja) 遺伝子の塩基置換の検査法

Legal Events

Date Code Title Description
PD00 Pending as of 2000-06-30 in czech republic
MM4A Patent lapsed due to non-payment of fee

Effective date: 20071127