CY1121738T1 - Μεθοδοι και συνθεσεις για στοχευμενη γενετικη τροποποιηση χρησιμοποιωντας συζευγμενα rna-οδηγους - Google Patents

Μεθοδοι και συνθεσεις για στοχευμενη γενετικη τροποποιηση χρησιμοποιωντας συζευγμενα rna-οδηγους

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Publication number
CY1121738T1
CY1121738T1 CY20191100640T CY191100640T CY1121738T1 CY 1121738 T1 CY1121738 T1 CY 1121738T1 CY 20191100640 T CY20191100640 T CY 20191100640T CY 191100640 T CY191100640 T CY 191100640T CY 1121738 T1 CY1121738 T1 CY 1121738T1
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Cyprus
Prior art keywords
methods
compositions
rnas
targeted gene
gene modification
Prior art date
Application number
CY20191100640T
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English (en)
Inventor
Andrew J. Murphy
David Frendewey
Ka-Man Venus Lai
Wojtek Auerbach
Gustavo Droguett
Anthony Gagliardi
David M. Valenzuela
Vera VORONINA
Lynn Macdonald
George D. Yancopoulos
Original Assignee
Regeneron Pharmaceuticals, Inc.
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Application filed by Regeneron Pharmaceuticals, Inc. filed Critical Regeneron Pharmaceuticals, Inc.
Publication of CY1121738T1 publication Critical patent/CY1121738T1/el

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Abstract

Παρέχονται συνθέσεις και μέθοδοι για τη δημιουργία και την προώθηση διαλληλικών στοχευμένων τροποποιήσεων σε γονιδιώματα με κύτταρα καθώς και για την παραγωγή μη ανθρώπινων ζώων που περιλαμβάνουν τα τροποποιημένα γονιδιώματα. Παρέχονται επίσης συνθέσεις και μέθοδοι για την τροποποίηση ενός γονιδιώματος εντός ενός κυττάρου, το οποίο είναι ετερόζυγο για ένα αλληλόμορφο, ώστε να γίνει ομόζυγο για το αλληλόμορφο αυτό. Οι μέθοδοι χρησιμοποιούν τις πρωτεΐνες Cas και δυο ή περισσότερα RNA-οδηγούς που στοχεύουν σε διαφορετικές θέσεις εντός του ίδιου γονιδιωματικού τόπου-στόχου. Παρέχονται επίσης μέθοδοι ταυτοποίησης κυττάρων με τροποποιημένα γονιδιώματα.
CY20191100640T 2014-11-21 2019-06-20 Μεθοδοι και συνθεσεις για στοχευμενη γενετικη τροποποιηση χρησιμοποιωντας συζευγμενα rna-οδηγους CY1121738T1 (el)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462083005P 2014-11-21 2014-11-21
US201562182314P 2015-06-19 2015-06-19
US201562211421P 2015-08-28 2015-08-28
PCT/US2015/062023 WO2016081923A2 (en) 2014-11-21 2015-11-20 METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAs

Publications (1)

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CY1121738T1 true CY1121738T1 (el) 2020-07-31

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US (4) US10457960B2 (el)
EP (2) EP3221457B1 (el)
JP (2) JP6727199B2 (el)
KR (3) KR20230070319A (el)
CN (2) CN113444747A (el)
AU (2) AU2015349692B2 (el)
BR (1) BR112017010547A2 (el)
CA (2) CA2968440A1 (el)
CY (1) CY1121738T1 (el)
DK (1) DK3221457T3 (el)
ES (1) ES2731437T3 (el)
HR (1) HRP20190949T1 (el)
HU (1) HUE044907T2 (el)
IL (3) IL301900A (el)
LT (1) LT3221457T (el)
MX (2) MX2017006670A (el)
NZ (1) NZ731962A (el)
PL (1) PL3221457T3 (el)
PT (1) PT3221457T (el)
RS (1) RS58893B1 (el)
RU (2) RU2734770C2 (el)
SG (2) SG10201913829YA (el)
SI (1) SI3221457T1 (el)
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