CY1118197T1 - Uk-2 βιοσυνθετικα γονιδια και μεθοδος για τη βελτιωση της παραγωγικοτητας σε uk-2 με χρηση των ιδιων - Google Patents
Uk-2 βιοσυνθετικα γονιδια και μεθοδος για τη βελτιωση της παραγωγικοτητας σε uk-2 με χρηση των ιδιωνInfo
- Publication number
- CY1118197T1 CY1118197T1 CY20161100725T CY161100725T CY1118197T1 CY 1118197 T1 CY1118197 T1 CY 1118197T1 CY 20161100725 T CY20161100725 T CY 20161100725T CY 161100725 T CY161100725 T CY 161100725T CY 1118197 T1 CY1118197 T1 CY 1118197T1
- Authority
- CY
- Cyprus
- Prior art keywords
- region
- biosynthetic gene
- strain
- dna
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- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
- C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Το γενωμικό DNA του Streptoverticillium sp. 3-7, το οποίο παράγει το UK-2, αναλύθηκε για την ταυτοποίηση μιας περιοχής αναμενόμενης να είναι ένα σύμπλεγμα UK-2 βιοσυνθετικών γονιδίων. Επιπλέον, με υβριδοποίηση αποικίας, απομονώθηκαν επιτυχώς τα DNA στην περιοχή. Περαιτέρω, τα DNA χρησιμοποιήθηκαν για την παρασκευή ενός στελέχους στο οποίο είχαν διασπαστεί τα γονίδια που υπήρχαν στην περιοχή. Το στέλεχος βρέθηκε ότι δεν παράγει UK-2. Επιβεβαιώθηκε ότι η γενωμική περιοχή ήταν το σύμπλεγμα UK-2 βιοσυνθετικών γονιδίων. Περαιτέρω, το Streptoverticillium sp. 3-7 μετασχηματίστηκε με εισαγωγή ενός φορέα στον οποίο είχε εισαχθεί το απομονωμένο σύμπλεγμα UK-2 βιοσυνθετικών γονιδίων. Βρέθηκε επίσης ότι η παραγωγικότητα σε UK-2 από το μετασχηματισμένο κύτταρο ήταν βελτιωμένη περίπου 10 έως 60 φορές ή περισσότερο σε σύγκριση με εκείνη του γονικού στελέχους. Επιπλέον, αποκαλύφθηκε ότι υπήρχαν 2 αντίγραφα του συμπλέγματος UK-2 βιοσυνθετικών γονιδίων ανά κύτταρο σε αυτά τα μετασχηματισμένα κύτταρα, αντιστοίχως.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012153986 | 2012-07-09 | ||
| PCT/JP2013/069081 WO2014010714A1 (en) | 2012-07-09 | 2013-07-08 | Uk-2 biosynthetic genes and method for improving uk-2 productivity using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1118197T1 true CY1118197T1 (el) | 2017-06-28 |
Family
ID=48874462
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20161100725T CY1118197T1 (el) | 2012-07-09 | 2016-07-22 | Uk-2 βιοσυνθετικα γονιδια και μεθοδος για τη βελτιωση της παραγωγικοτητας σε uk-2 με χρηση των ιδιων |
Country Status (28)
| Country | Link |
|---|---|
| US (2) | US9365879B2 (el) |
| EP (1) | EP2731435B1 (el) |
| JP (1) | JP6263133B2 (el) |
| KR (1) | KR102159415B1 (el) |
| CN (1) | CN104427870B (el) |
| AU (1) | AU2013287626B2 (el) |
| BR (1) | BR112015000282B1 (el) |
| CA (1) | CA2878644C (el) |
| CR (1) | CR20150052A (el) |
| CY (1) | CY1118197T1 (el) |
| DK (1) | DK2731435T3 (el) |
| EA (1) | EA028269B1 (el) |
| EC (1) | ECSP15001894A (el) |
| ES (1) | ES2592405T3 (el) |
| GT (1) | GT201400294A (el) |
| IL (1) | IL236532B (el) |
| IN (1) | IN2014KN03120A (el) |
| MA (1) | MA37790B1 (el) |
| MX (1) | MX358867B (el) |
| NZ (1) | NZ703756A (el) |
| PE (1) | PE20150753A1 (el) |
| PH (1) | PH12015500004B1 (el) |
| PT (1) | PT2731435T (el) |
| TN (1) | TN2014000538A1 (el) |
| TW (1) | TWI601822B (el) |
| UA (1) | UA116989C2 (el) |
| WO (1) | WO2014010714A1 (el) |
| ZA (1) | ZA201500470B (el) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017205387A1 (en) * | 2016-05-23 | 2017-11-30 | Northwestern University | Systems and methods for untargeted metabolomic screening |
| CN115261291B (zh) * | 2021-04-29 | 2023-10-20 | 上海医药工业研究院 | 一种生产uk 2a的基因工程菌及其制备方法和应用 |
| CN114717208B (zh) * | 2022-05-18 | 2024-07-09 | 上海交通大学 | 一种酰基CoA合成酶及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3526602B2 (ja) | 1994-02-24 | 2004-05-17 | サントリー株式会社 | 新規抗真菌化合物 |
| AU8887898A (en) | 1997-08-29 | 1999-03-22 | Meiji Seika Kaisha Ltd. | Rice blast control agent and wheat scab control agent |
| NZ506249A (en) | 1998-02-06 | 2003-04-29 | Meiji Seika Kaisha | Antifungal compounds and process for producing the same from UK-2 |
| US7285404B1 (en) | 1999-09-07 | 2007-10-23 | Meiji Seika Kaisha, Ltd. | Cyclic depsipeptide synthetase and method for recombinant production |
| AR037328A1 (es) * | 2001-10-23 | 2004-11-03 | Dow Agrosciences Llc | Compuesto de [7-bencil-2,6-dioxo-1,5-dioxonan-3-il]-4-metoxipiridin-2-carboxamida, composicion que lo comprende y metodo que lo utiliza |
-
2013
- 2013-07-08 WO PCT/JP2013/069081 patent/WO2014010714A1/en active Application Filing
- 2013-07-08 MX MX2014015928A patent/MX358867B/es active IP Right Grant
- 2013-07-08 JP JP2014560583A patent/JP6263133B2/ja active Active
- 2013-07-08 PE PE2014002494A patent/PE20150753A1/es active IP Right Grant
- 2013-07-08 EA EA201590170A patent/EA028269B1/ru unknown
- 2013-07-08 DK DK13740383.8T patent/DK2731435T3/en active
- 2013-07-08 IN IN3120KON2014 patent/IN2014KN03120A/en unknown
- 2013-07-08 MA MA37790A patent/MA37790B1/fr unknown
- 2013-07-08 EP EP13740383.8A patent/EP2731435B1/en active Active
- 2013-07-08 BR BR112015000282-0A patent/BR112015000282B1/pt active IP Right Grant
- 2013-07-08 CN CN201380036753.1A patent/CN104427870B/zh active Active
- 2013-07-08 KR KR1020147036291A patent/KR102159415B1/ko active IP Right Grant
- 2013-07-08 ES ES13740383.8T patent/ES2592405T3/es active Active
- 2013-07-08 AU AU2013287626A patent/AU2013287626B2/en active Active
- 2013-07-08 CA CA2878644A patent/CA2878644C/en active Active
- 2013-07-08 US US13/936,771 patent/US9365879B2/en active Active
- 2013-07-08 NZ NZ703756A patent/NZ703756A/en unknown
- 2013-07-08 PT PT137403838T patent/PT2731435T/pt unknown
- 2013-07-09 TW TW102124528A patent/TWI601822B/zh active
- 2013-08-07 UA UAA201500953A patent/UA116989C2/uk unknown
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2014
- 2014-12-18 GT GT201400294A patent/GT201400294A/es unknown
- 2014-12-26 TN TN2014000538A patent/TN2014000538A1/fr unknown
- 2014-12-31 IL IL236532A patent/IL236532B/en active IP Right Grant
-
2015
- 2015-01-05 PH PH12015500004A patent/PH12015500004B1/en unknown
- 2015-01-19 EC ECIEPI20151894A patent/ECSP15001894A/es unknown
- 2015-01-22 ZA ZA2015/00470A patent/ZA201500470B/en unknown
- 2015-02-05 CR CR20150052A patent/CR20150052A/es unknown
-
2016
- 2016-05-10 US US15/150,887 patent/US9771606B2/en active Active
- 2016-07-22 CY CY20161100725T patent/CY1118197T1/el unknown
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