PE20150753A1 - Genes biosinteticos uk-2 y metodo para mejorar la productividad de uk-2 usando los mismos - Google Patents
Genes biosinteticos uk-2 y metodo para mejorar la productividad de uk-2 usando los mismosInfo
- Publication number
- PE20150753A1 PE20150753A1 PE2014002494A PE2014002494A PE20150753A1 PE 20150753 A1 PE20150753 A1 PE 20150753A1 PE 2014002494 A PE2014002494 A PE 2014002494A PE 2014002494 A PE2014002494 A PE 2014002494A PE 20150753 A1 PE20150753 A1 PE 20150753A1
- Authority
- PE
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- Prior art keywords
- region
- productivity
- biosynthetic
- strain
- genes
- Prior art date
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
- C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La invencion proporciona un metodo de produccion con capacidad de produccion en masa de UK-2 a bajo costo, se analizo el ADN genomico de Streptoverticillium sp. 3-7, el cual produce UK-2, para identificar una region que se espera sea un grupo de genes biosinteticos de UK-2. Por otra parte, por hibridacion por colonias, se aislaron exitosamente los ADN en la region. Ademas, se utilizaron los ADN para preparar una cepa en la cual se alteraron los genes presentes en la region. Se encontro que la cepa no produce UK-2. Se verifico que la region genomica fuera el grupo de genes biosinteticos de UK-2. Ademas, se transformo Streptoverticillium sp. 3-7 por la introduccion de un vector en el cual se inserto el grupo de genes biosinteticos de UK-2 aislados. Tambien se encontro que la productividad de UK-2 por el transformante mejoro alrededor de 10 a 60 veces o mas en comparacion con aquel de la cepa progenitora. Por otra parte, se revelo que las dos copias del grupo de genes biosinteticos de UK-2 estuvieron presentes estos transformantes, respectivamente
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012153986 | 2012-07-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PE20150753A1 true PE20150753A1 (es) | 2015-05-17 |
Family
ID=48874462
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PE2014002494A PE20150753A1 (es) | 2012-07-09 | 2013-07-08 | Genes biosinteticos uk-2 y metodo para mejorar la productividad de uk-2 usando los mismos |
Country Status (28)
| Country | Link |
|---|---|
| US (2) | US9365879B2 (es) |
| EP (1) | EP2731435B1 (es) |
| JP (1) | JP6263133B2 (es) |
| KR (1) | KR102159415B1 (es) |
| CN (1) | CN104427870B (es) |
| AU (1) | AU2013287626B2 (es) |
| BR (1) | BR112015000282B1 (es) |
| CA (1) | CA2878644C (es) |
| CR (1) | CR20150052A (es) |
| CY (1) | CY1118197T1 (es) |
| DK (1) | DK2731435T3 (es) |
| EA (1) | EA028269B1 (es) |
| EC (1) | ECSP15001894A (es) |
| ES (1) | ES2592405T3 (es) |
| GT (1) | GT201400294A (es) |
| IL (1) | IL236532B (es) |
| IN (1) | IN2014KN03120A (es) |
| MA (1) | MA37790B1 (es) |
| MX (1) | MX358867B (es) |
| NZ (1) | NZ703756A (es) |
| PE (1) | PE20150753A1 (es) |
| PH (1) | PH12015500004B1 (es) |
| PT (1) | PT2731435T (es) |
| TN (1) | TN2014000538A1 (es) |
| TW (1) | TWI601822B (es) |
| UA (1) | UA116989C2 (es) |
| WO (1) | WO2014010714A1 (es) |
| ZA (1) | ZA201500470B (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10808256B2 (en) | 2016-05-23 | 2020-10-20 | Northwestern University | Systems and methods for untargeted metabolomic screening |
| CN115261291B (zh) * | 2021-04-29 | 2023-10-20 | 上海医药工业研究院 | 一种生产uk 2a的基因工程菌及其制备方法和应用 |
| CN114717208A (zh) * | 2022-05-18 | 2022-07-08 | 上海交通大学 | 一种酰基CoA合成酶及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3526602B2 (ja) | 1994-02-24 | 2004-05-17 | サントリー株式会社 | 新規抗真菌化合物 |
| WO1999011127A1 (fr) | 1997-08-29 | 1999-03-11 | Meiji Seika Kaisha, Ltd. | Agent de lutte contre la piriculariose du riz et agent de lutte contre la tavelure du ble |
| AU751098B2 (en) | 1998-02-06 | 2002-08-08 | Meiji Seika Kaisha Ltd. | Novel antifungal compounds and process for producing the same |
| KR100702728B1 (ko) | 1999-09-07 | 2007-04-03 | 메이지 세이카 가부시키가이샤 | 환상 뎁시펩티드 합성효소 및 그의 유전자 및 환상뎁시펩티드의 대량생산계 |
| AR037328A1 (es) * | 2001-10-23 | 2004-11-03 | Dow Agrosciences Llc | Compuesto de [7-bencil-2,6-dioxo-1,5-dioxonan-3-il]-4-metoxipiridin-2-carboxamida, composicion que lo comprende y metodo que lo utiliza |
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2013
- 2013-07-08 NZ NZ703756A patent/NZ703756A/en unknown
- 2013-07-08 WO PCT/JP2013/069081 patent/WO2014010714A1/en active Application Filing
- 2013-07-08 BR BR112015000282-0A patent/BR112015000282B1/pt active IP Right Grant
- 2013-07-08 IN IN3120KON2014 patent/IN2014KN03120A/en unknown
- 2013-07-08 ES ES13740383.8T patent/ES2592405T3/es active Active
- 2013-07-08 EP EP13740383.8A patent/EP2731435B1/en active Active
- 2013-07-08 JP JP2014560583A patent/JP6263133B2/ja active Active
- 2013-07-08 EA EA201590170A patent/EA028269B1/ru unknown
- 2013-07-08 MX MX2014015928A patent/MX358867B/es active IP Right Grant
- 2013-07-08 AU AU2013287626A patent/AU2013287626B2/en active Active
- 2013-07-08 CN CN201380036753.1A patent/CN104427870B/zh active Active
- 2013-07-08 KR KR1020147036291A patent/KR102159415B1/ko active IP Right Grant
- 2013-07-08 US US13/936,771 patent/US9365879B2/en active Active
- 2013-07-08 DK DK13740383.8T patent/DK2731435T3/en active
- 2013-07-08 PT PT137403838T patent/PT2731435T/pt unknown
- 2013-07-08 MA MA37790A patent/MA37790B1/fr unknown
- 2013-07-08 CA CA2878644A patent/CA2878644C/en active Active
- 2013-07-08 PE PE2014002494A patent/PE20150753A1/es active IP Right Grant
- 2013-07-09 TW TW102124528A patent/TWI601822B/zh active
- 2013-08-07 UA UAA201500953A patent/UA116989C2/uk unknown
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2014
- 2014-12-18 GT GT201400294A patent/GT201400294A/es unknown
- 2014-12-26 TN TN2014000538A patent/TN2014000538A1/fr unknown
- 2014-12-31 IL IL236532A patent/IL236532B/en active IP Right Grant
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2015
- 2015-01-05 PH PH12015500004A patent/PH12015500004B1/en unknown
- 2015-01-19 EC ECIEPI20151894A patent/ECSP15001894A/es unknown
- 2015-01-22 ZA ZA2015/00470A patent/ZA201500470B/en unknown
- 2015-02-05 CR CR20150052A patent/CR20150052A/es unknown
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2016
- 2016-05-10 US US15/150,887 patent/US9771606B2/en active Active
- 2016-07-22 CY CY20161100725T patent/CY1118197T1/el unknown
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