EA201591447A1 - Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов - Google Patents

Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов

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EA201591447A1
EA201591447A1 EA201591447A EA201591447A EA201591447A1 EA 201591447 A1 EA201591447 A1 EA 201591447A1 EA 201591447 A EA201591447 A EA 201591447A EA 201591447 A EA201591447 A EA 201591447A EA 201591447 A1 EA201591447 A1 EA 201591447A1
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Eurasian Patent Office
Prior art keywords
genes
compositions
efficiency
improving
methods
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EA201591447A
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English (en)
Inventor
Питер Р. Битэм
Грегори Ф.В. Гокал
Кристиан Шопке
Ноэль Джой Сойер
Джеймс Пирс
Роза Е. Сегами
Джерри Мозорук
Original Assignee
Сибас Юс Ллс
Сибас Юроп Б.В.
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Application filed by Сибас Юс Ллс, Сибас Юроп Б.В. filed Critical Сибас Юс Ллс
Publication of EA201591447A1 publication Critical patent/EA201591447A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

В настоящем изобретении предложены улучшенные способы модификации генов в клетках растения и растения и семена, полученные из них. В частности, настоящее изобретение относится к повышенной эффективности направленного мутирования генов путем комбинирования олигонуклеотидов для репарации генов с подходами, которые повышают доступность компонентов механизмов репарации генов в целевой клетке.
EA201591447A 2013-03-15 2014-03-14 Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов EA201591447A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361801320P 2013-03-15 2013-03-15
PCT/US2014/029621 WO2014144987A2 (en) 2013-03-15 2014-03-14 Methods and compositions for increasing efficiency of increased efficiency of targeted gene modification using oligonucleotide-mediated gene repair

Publications (1)

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EA201591447A1 true EA201591447A1 (ru) 2016-05-31

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EA201591447A EA201591447A1 (ru) 2013-03-15 2014-03-14 Способы и композиции для повышения эффективности направленной модификации генов с применением опосредованной олигонуклеотидами репарации генов

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US (2) US11434494B2 (ru)
EP (1) EP2966984B1 (ru)
JP (1) JP2016516408A (ru)
KR (1) KR102304487B1 (ru)
CN (1) CN105338805A (ru)
AU (2) AU2014233465A1 (ru)
BR (1) BR112015022848B1 (ru)
CA (1) CA2905135C (ru)
CL (1) CL2015002650A1 (ru)
DK (1) DK2966984T3 (ru)
EA (1) EA201591447A1 (ru)
ES (1) ES2912400T3 (ru)
HK (1) HK1220864A1 (ru)
HR (1) HRP20220521T1 (ru)
HU (1) HUE058351T2 (ru)
IL (1) IL241145B (ru)
LT (1) LT2966984T (ru)
MY (1) MY191390A (ru)
PL (1) PL2966984T3 (ru)
PT (1) PT2966984T (ru)
RS (1) RS63188B1 (ru)
SI (1) SI2966984T1 (ru)
UA (1) UA121099C2 (ru)
WO (1) WO2014144987A2 (ru)

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MY191390A (en) 2022-06-22
DK2966984T3 (da) 2022-04-25
AU2020220059A1 (en) 2020-09-03
SI2966984T1 (sl) 2022-06-30
LT2966984T (lt) 2022-05-25
EP2966984A2 (en) 2016-01-20
US11434494B2 (en) 2022-09-06
WO2014144987A3 (en) 2014-12-04
US20230141977A1 (en) 2023-05-11
HRP20220521T1 (hr) 2022-06-10
EP2966984B1 (en) 2022-03-02
KR102304487B1 (ko) 2021-09-24
JP2016516408A (ja) 2016-06-09
CA2905135C (en) 2022-10-25
BR112015022848A2 (pt) 2017-11-07
KR20150132202A (ko) 2015-11-25
EP2966984A4 (en) 2016-09-21
RS63188B1 (sr) 2022-06-30
CN105338805A (zh) 2016-02-17
AU2014233465A1 (en) 2015-09-03
ES2912400T3 (es) 2022-05-25
US20160304892A1 (en) 2016-10-20
IL241145B (en) 2022-07-01
UA121099C2 (uk) 2020-04-10
PL2966984T3 (pl) 2022-06-13
HK1220864A1 (zh) 2017-05-19
CL2015002650A1 (es) 2016-03-28
AU2020220059B2 (en) 2023-06-15
NZ711146A (en) 2021-08-27
WO2014144987A2 (en) 2014-09-18
IL241145A0 (en) 2015-11-30
PT2966984T (pt) 2022-05-05
CA2905135A1 (en) 2014-09-18
BR112015022848B1 (pt) 2022-10-11
HUE058351T2 (hu) 2022-07-28

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