BR112015022848A2 - métodos e composições para aumentar a eficiência de maior eficiência de modificação genética visada usando o reparo de genes mediada por oligonucleotídeo - Google Patents

métodos e composições para aumentar a eficiência de maior eficiência de modificação genética visada usando o reparo de genes mediada por oligonucleotídeo

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Publication number
BR112015022848A2
BR112015022848A2 BR112015022848A BR112015022848A BR112015022848A2 BR 112015022848 A2 BR112015022848 A2 BR 112015022848A2 BR 112015022848 A BR112015022848 A BR 112015022848A BR 112015022848 A BR112015022848 A BR 112015022848A BR 112015022848 A2 BR112015022848 A2 BR 112015022848A2
Authority
BR
Brazil
Prior art keywords
efficiency
increasing
oligonucleotide
compositions
gene repair
Prior art date
Application number
BR112015022848A
Other languages
English (en)
Other versions
BR112015022848B1 (pt
Inventor
Schopke Christian
F W Gocal Gregory
Pearce James
Mozoruk Jerry
Joy Sauer Noel
R Beetham Peter
E Segami Rosa
Original Assignee
Cibus Europe Bv
Cibus Us Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Cibus Europe Bv, Cibus Us Llc filed Critical Cibus Europe Bv
Publication of BR112015022848A2 publication Critical patent/BR112015022848A2/pt
Publication of BR112015022848B1 publication Critical patent/BR112015022848B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

resumo métodos e composições para aumentar a eficiência de maior eficiência de modificação genética visada usando o reparo de genes mediada por oligonucleotídeo a invenção provê para métodos aperfeiçoados a modificação de genes em células vegetais, e plantas e sementes derivados desta. mais especificamente, a invenção se refere ao aumento da eficiência da mutação do gene alvo combinando oligonucleotídeos de reparação de gene com abordagens que melhoram a disponibilidade de componentes dos mecanismos de reparação de gene de célula alvo. 1/1
BR112015022848-8A 2013-03-15 2014-03-14 Método para introdução de uma mutação mediada por gron em uma sequência de dna alvo em um protoplasto vegetal e uso de uma ou mais endonucleases sítio específica que introduzem cortes de fita única BR112015022848B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361801320P 2013-03-15 2013-03-15
US61/801,320 2013-03-15
PCT/US2014/029621 WO2014144987A2 (en) 2013-03-15 2014-03-14 Methods and compositions for increasing efficiency of increased efficiency of targeted gene modification using oligonucleotide-mediated gene repair

Publications (2)

Publication Number Publication Date
BR112015022848A2 true BR112015022848A2 (pt) 2017-11-07
BR112015022848B1 BR112015022848B1 (pt) 2022-10-11

Family

ID=51538403

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022848-8A BR112015022848B1 (pt) 2013-03-15 2014-03-14 Método para introdução de uma mutação mediada por gron em uma sequência de dna alvo em um protoplasto vegetal e uso de uma ou mais endonucleases sítio específica que introduzem cortes de fita única

Country Status (24)

Country Link
US (2) US11434494B2 (pt)
EP (1) EP2966984B1 (pt)
JP (1) JP2016516408A (pt)
KR (1) KR102304487B1 (pt)
CN (1) CN105338805A (pt)
AU (2) AU2014233465A1 (pt)
BR (1) BR112015022848B1 (pt)
CA (1) CA2905135C (pt)
CL (1) CL2015002650A1 (pt)
DK (1) DK2966984T3 (pt)
EA (1) EA201591447A1 (pt)
ES (1) ES2912400T3 (pt)
HK (1) HK1220864A1 (pt)
HR (1) HRP20220521T1 (pt)
HU (1) HUE058351T2 (pt)
IL (1) IL241145B (pt)
LT (1) LT2966984T (pt)
MY (1) MY191390A (pt)
PL (1) PL2966984T3 (pt)
PT (1) PT2966984T (pt)
RS (1) RS63188B1 (pt)
SI (1) SI2966984T1 (pt)
UA (1) UA121099C2 (pt)
WO (1) WO2014144987A2 (pt)

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PL2966984T3 (pl) 2013-03-15 2022-06-13 Cibus Us Llc Ukierunkowana modyfikacja genu z użyciem naprawy genu, w której pośredniczy oligonukleotyd
CA2997535A1 (en) * 2015-07-23 2017-01-26 Mayo Foundation For Medical Education And Research Editing mitochondrial dna
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
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JP2017205104A (ja) 2016-05-13 2017-11-24 株式会社カネカ 植物のゲノム編集方法
CN106755067A (zh) * 2016-12-05 2017-05-31 天津吉诺沃生物科技有限公司 通过瞬时表达对植物基因进行定点插入的方法及获得的瞬时表达细胞、组织和突变植株
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA

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Also Published As

Publication number Publication date
MY191390A (en) 2022-06-22
HRP20220521T1 (hr) 2022-06-10
KR102304487B1 (ko) 2021-09-24
PT2966984T (pt) 2022-05-05
CL2015002650A1 (es) 2016-03-28
AU2020220059B2 (en) 2023-06-15
EP2966984B1 (en) 2022-03-02
HUE058351T2 (hu) 2022-07-28
SI2966984T1 (sl) 2022-06-30
CA2905135C (en) 2022-10-25
IL241145B (en) 2022-07-01
US20230141977A1 (en) 2023-05-11
PL2966984T3 (pl) 2022-06-13
UA121099C2 (uk) 2020-04-10
DK2966984T3 (da) 2022-04-25
JP2016516408A (ja) 2016-06-09
IL241145A0 (en) 2015-11-30
US20160304892A1 (en) 2016-10-20
EP2966984A2 (en) 2016-01-20
AU2020220059A1 (en) 2020-09-03
RS63188B1 (sr) 2022-06-30
AU2014233465A1 (en) 2015-09-03
EA201591447A1 (ru) 2016-05-31
ES2912400T3 (es) 2022-05-25
HK1220864A1 (zh) 2017-05-19
NZ711146A (en) 2021-08-27
CA2905135A1 (en) 2014-09-18
CN105338805A (zh) 2016-02-17
WO2014144987A3 (en) 2014-12-04
EP2966984A4 (en) 2016-09-21
KR20150132202A (ko) 2015-11-25
LT2966984T (lt) 2022-05-25
BR112015022848B1 (pt) 2022-10-11
US11434494B2 (en) 2022-09-06
WO2014144987A2 (en) 2014-09-18

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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