CL2018002140A1 - Métodos y composiciones para aumentar la eficiencia de modificación genética dirigida usando reparación genética mediada por oligonucleótidos - Google Patents

Métodos y composiciones para aumentar la eficiencia de modificación genética dirigida usando reparación genética mediada por oligonucleótidos

Info

Publication number
CL2018002140A1
CL2018002140A1 CL2018002140A CL2018002140A CL2018002140A1 CL 2018002140 A1 CL2018002140 A1 CL 2018002140A1 CL 2018002140 A CL2018002140 A CL 2018002140A CL 2018002140 A CL2018002140 A CL 2018002140A CL 2018002140 A1 CL2018002140 A1 CL 2018002140A1
Authority
CL
Chile
Prior art keywords
compositions
methods
oligonucleotide
increasing
efficiency
Prior art date
Application number
CL2018002140A
Other languages
English (en)
Inventor
Peter R Beetham
Gregory F W Gocal
Christian Schopke
James Pearce
Noel Sauer
Rosa E Segami
Jerry Mozoruk
Original Assignee
Cibus Europe Bv
Cibus Us Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cibus Europe Bv, Cibus Us Llc filed Critical Cibus Europe Bv
Publication of CL2018002140A1 publication Critical patent/CL2018002140A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

Abstract

EN LA PRESENTE SE INCLUYEN MÉTODOS Y COMPOSICIONES PARA REALIZAR CAMBIOS DIRIGIDOS EN UNA SECUENCIA DE ADN. EN DIVERSOS ASPECTOS Y REALIZACIONES, SE PROPORCIONAN MÉTODOS Y COMPOSICIONES PARA MODIFICAR UNA SECUENCIA DE ADN EN UNA CÉLULA (COMO UNA CÉLULA VEGETAL, BACTERIANA, DE LEVADURA, FÚNGICA, DE ALGAS O DE MAMÍFERO). EN ALGUNOS ASPECTOS Y REALIZACIONES, LA MODIFICACIÓN DEL ADN INVOLUCRA LA COMBINACIÓN DE OLIGONUCLEÓTIDOS DE REPARACIÓN GENÉTICA CON ENFOQUES QUE MEJORAN LA DISPONIBILIDAD DE COMPONENTES DE LOS MECANISMOS DE REPARACIÓN GENÉTICA DE LA CÉLULA DIANA, COMO UN CORTADOR DE ADN.
CL2018002140A 2016-02-09 2018-08-08 Métodos y composiciones para aumentar la eficiencia de modificación genética dirigida usando reparación genética mediada por oligonucleótidos CL2018002140A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201662293278P 2016-02-09 2016-02-09

Publications (1)

Publication Number Publication Date
CL2018002140A1 true CL2018002140A1 (es) 2019-01-04

Family

ID=59563243

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2018002140A CL2018002140A1 (es) 2016-02-09 2018-08-08 Métodos y composiciones para aumentar la eficiencia de modificación genética dirigida usando reparación genética mediada por oligonucleótidos

Country Status (9)

Country Link
US (2) US11542515B2 (es)
JP (2) JP2019506170A (es)
KR (1) KR20180116769A (es)
CN (1) CN109072248A (es)
BR (1) BR112018016278A2 (es)
CA (1) CA3014036A1 (es)
CL (1) CL2018002140A1 (es)
EA (1) EA201891629A1 (es)
WO (1) WO2017138986A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018115389A1 (en) * 2016-12-22 2018-06-28 Keygene N.V. Methods of targeted genetic alteration in plant cells
CN107474129B (zh) * 2017-10-12 2018-10-19 江西汉氏联合干细胞科技有限公司 特异性增强crispr-cas系统基因编辑效率的方法
CN111787788B (zh) 2017-12-28 2024-03-19 嘉士伯有限公司 具有改善的细胞壁性质的谷类植物
EP3731628A1 (en) 2017-12-28 2020-11-04 Carlsberg A/S Barley with increased hydrolytic enzyme activity
WO2019134962A1 (en) 2018-01-05 2019-07-11 Carlsberg A/S Cereal comprising starch with low gelatinisation temperature
AU2019247403A1 (en) * 2018-04-04 2020-11-26 Cibus Europe, B.V. FAD2 genes and mutations
WO2020184403A1 (ja) * 2019-03-08 2020-09-17 公立大学法人福島県立医科大学 培養細胞の生産方法
EP3785529A1 (en) 2019-08-28 2021-03-03 Carlsberg A/S Barley plants with altered protein content in grains
US20230111237A1 (en) 2020-03-02 2023-04-13 Carlsberg A/S Barley Plants with High Limit Dextrinase Activity
WO2021183504A1 (en) * 2020-03-11 2021-09-16 North Carolina State University Compositions, methods, and systems for genome editing technology
CN112375781B (zh) * 2020-11-08 2023-05-02 浙江大学 pC1300-MAS-Cas9基因编辑系统在84K杨树基因编辑中的应用
WO2022232840A1 (en) * 2021-04-29 2022-11-03 The Regents Of The University Of California Systems and methods for genetic editing with increased efficiency
WO2023006699A1 (en) 2021-07-30 2023-02-02 Københavns Universitet Cells and method for producing isoprenoid molecules with canonical and non-canonical structures

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5024944A (en) 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5008200A (en) 1988-05-08 1991-04-16 United Agriseeds, Inc. Propagating multiple whole fertile plants from immature leguminous
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
ATE225853T1 (de) 1990-04-12 2002-10-15 Syngenta Participations Ag Gewebe-spezifische promotoren
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5219746A (en) 1990-07-19 1993-06-15 Chris Brinegar Ice-mediated introduction of substances into biological material
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
US5641625A (en) 1992-05-22 1997-06-24 Isis Pharmaceuticals, Inc. Cleaving double-stranded DNA with peptide nucleic acids
DE4216134A1 (de) 1991-06-20 1992-12-24 Europ Lab Molekularbiolog Synthetische katalytische oligonukleotidstrukturen
AU668096B2 (en) 1991-08-27 1996-04-26 Syngenta Participations Ag Proteins with insecticidal properties against homopteran insects and their use in plant protection
TW261517B (es) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5593874A (en) 1992-03-19 1997-01-14 Monsanto Company Enhanced expression in plants
IT230274Y1 (it) 1993-06-11 1999-06-02 Silc Spa Pannolone assorbente sagomato per incontinenza
US5962426A (en) 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
PT733059E (pt) 1993-12-09 2001-03-30 Univ Jefferson Compostos e metodos para mutacoes dirigidas ao local em celulas eucarioticas
ATE244723T1 (de) 1994-04-27 2003-07-15 Novartis Pharma Gmbh Nukleoside und oligonukleotide mit 2'- ethergruppen
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US5760012A (en) 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations
US5731181A (en) 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
US5888983A (en) 1996-05-01 1999-03-30 Thomas Jefferson University Method and oligonucleobase compounds for curing diseases caused by mutations
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US6524613B1 (en) 1997-04-30 2003-02-25 Regents Of The University Of Minnesota Hepatocellular chimeraplasty
EP0979311A1 (en) 1997-04-30 2000-02-16 Of The University Of Minnesota Regents $i(IN VIVO) USE OF RECOMBINAGENIC OLIGONUCLEOBASES TO CORRECT GENETIC LESIONS IN HEPATOCYTES
JP2001512687A (ja) 1997-08-05 2001-08-28 キメラジェン，インコーポレーテッド 植物に局在性遺伝子変化を生じさせるための混合二本鎖オリゴヌクレオチドの使用
EP1019516B1 (en) 1997-10-07 2004-06-09 EMBRAPA-Empresa Brasileira De Pesquisa Agropecuaria A process for obtaining transgenic leguminous plants (leguminosae) containing exogenous dna
DE69920879T2 (de) 1998-02-26 2005-10-13 Pioneer Hi-Bred International, Inc. Konstitutive maispromotoren
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
US6004804A (en) 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US7013219B2 (en) 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7070934B2 (en) 1999-01-12 2006-07-04 Sangamo Biosciences, Inc. Ligand-controlled regulation of endogenous gene expression
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6271360B1 (en) 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
DK1353941T3 (da) 2001-01-22 2013-06-17 Sangamo Biosciences Inc Modificerede zinkfingerbindingsproteiner
US20030115641A1 (en) 2001-07-24 2003-06-19 Dobres Michael S. Transformation of plants by electroporation of cultured explants
US20030084473A1 (en) * 2001-08-09 2003-05-01 Valigen Non-transgenic herbicide resistant plants
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
US7361635B2 (en) 2002-08-29 2008-04-22 Sangamo Biosciences, Inc. Simultaneous modulation of multiple genes
EP4174179A3 (en) 2005-08-23 2023-09-27 The Trustees of the University of Pennsylvania Rna containing modified nucleosides and methods of use thereof
WO2007073149A1 (en) 2005-12-22 2007-06-28 Keygene N.V. Alternative nucleotides for improved targeted nucleotide exchange
EP2923563A3 (en) * 2006-01-12 2015-10-14 Cibus Europe B.V. EPSPS mutants
CA2693525C (en) 2007-06-07 2020-09-29 Agriculture And Agri-Food Canada Nanocarrier based transfection and transduction of plant gametophytic cells
US20090205064A1 (en) * 2007-10-05 2009-08-13 Christian Schopke Mutated Acetohydroxyacid Synthase Genes in Brassica
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
SG187075A1 (en) * 2010-07-23 2013-03-28 Sigma Aldrich Co Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
US20120122223A1 (en) 2010-08-03 2012-05-17 Cibus Us Llc Mutated protoporphyrinogen ix oxidase (ppx) genes
US20140298508A1 (en) * 2010-12-23 2014-10-02 Philip Morris Products S.A. Alpha-mannosidases from plants and methods for using the same
PE20190844A1 (es) 2012-05-25 2019-06-17 Emmanuelle Charpentier Modulacion de transcripcion con arn de direccion a adn generico
CA2876426A1 (en) * 2012-06-15 2013-12-19 E. I. Du Pont De Nemours And Company Methods and compositions involving als variants with native substrate preference
CN102850444B (zh) * 2012-07-21 2015-02-18 上海斯丹赛生物技术有限公司 一对转录激活子样效应因子核酸酶l3和r1及其编码基因与应用
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US9580722B2 (en) 2013-03-01 2017-02-28 North Carolina State University Transcription factors that regulate nicotine biosynthesis in tobacco
US9957515B2 (en) * 2013-03-15 2018-05-01 Cibus Us Llc Methods and compositions for targeted gene modification
BR112016003561B8 (pt) * 2013-08-22 2022-11-01 Du Pont Método para a produção de uma modificação genética, método para a introdução de um polinucleotídeo de interesse no genoma de uma planta, método para a edição de um segundo gene em um genoma de planta e método para gerar uma planta de milho resistente ao glifosato
CA3208184A1 (en) * 2014-03-14 2015-09-17 Cibus Us Llc Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
US10676754B2 (en) * 2014-07-11 2020-06-09 E I Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide

Also Published As

Publication number Publication date
US20230407322A1 (en) 2023-12-21
CA3014036A1 (en) 2017-08-17
JP2019506170A (ja) 2019-03-07
JP2022153467A (ja) 2022-10-12
US20190249182A1 (en) 2019-08-15
CN109072248A (zh) 2018-12-21
BR112018016278A2 (pt) 2019-01-02
WO2017138986A1 (en) 2017-08-17
KR20180116769A (ko) 2018-10-25
US11542515B2 (en) 2023-01-03
EA201891629A1 (ru) 2019-03-29

Similar Documents

Publication Publication Date Title
CL2018002140A1 (es) Métodos y composiciones para aumentar la eficiencia de modificación genética dirigida usando reparación genética mediada por oligonucleótidos
CL2016002316A1 (es) Método para provocar un cambio genético en una célula vegetal, basado en someter dicha célula a un corte de adn y una gron modificada.
CL2015002648A1 (es) Métodos y composiciones para aumentar la eficacia de la modificación génica dirigida utilizando reparación génica mediada por oligonucleótidos
CL2018000194A1 (es) Compuestos de piridina
UY35812A (es) ?loci de maíz óptimos?.
CR20130592A (es) Composiciones y métodos para transferencia de energía mitocondrial de línea germinal autóloga
UY35814A (es) ?lugares óptimos para la soja?.
UY34100A (es) INMUNOCONJUGADOS DEL PROFARMACO seco-MGBA, COMPOSICIONES Y MÉTODOS PARA PREPARARLOS, Y SUS USOS
AR085533A1 (es) Metodos de produccion de un locus de rasgos transgenicos complejo
AR105776A1 (es) Recombinación mejorada de loci genómicos
RS54073B1 (en) STROMAL MATERIAL CELLS
AR111033A1 (es) Mezcla controlada de biodiésel en flujos destilados
MX2020003605A (es) Manipulación del metabolismo de triptamina.
CL2015002650A1 (es) Métodos y composiciones para aumentar la eficacia de la modificación génica, dirigida utilizando reparación génica mediada por oligonucleótidos.
CY1123144T1 (el) Επαγωγη καρδιακης αναγεννησης μe microrna
AR095348A1 (es) Medios de cultivo celular y métodos de producción de anticuerpos
BR112016028182A2 (pt) método para produzir um fragmento de fragmento cristalizável, e, fragmento fc.
MX2020009670A (es) Metodos de cultivo por perfusion y usos de los mismos.
BR112013026093A2 (pt) mistura de combustível renovável de baixo ponto de escoamento
CL2020000734A1 (es) Expansión y uso de fracciones de células nk ampliadas.
WO2015171852A3 (en) Methods and compositions for non-cytotoxic stem cell transplantation
UY35966A (es) ?elementos reguladores de zea mays y usos de estos?.
MX2016012102A (es) Metodos relacionados con celulas pluripotentes.
BR112015017830A2 (pt) processos e meios para aumentar a tolerância ao estresse e a biomassa em plantas
AR090250A1 (es) Factores de transcripcion en las plantas relacionadas con niveles de nitrato y metodos para usar los mismos