AR088562A1 - Metodos y composiciones para silenciar genes usando micro arn artificiales - Google Patents

Metodos y composiciones para silenciar genes usando micro arn artificiales

Info

Publication number
AR088562A1
AR088562A1 ARP120104034A ARP120104034A AR088562A1 AR 088562 A1 AR088562 A1 AR 088562A1 AR P120104034 A ARP120104034 A AR P120104034A AR P120104034 A ARP120104034 A AR P120104034A AR 088562 A1 AR088562 A1 AR 088562A1
Authority
AR
Argentina
Prior art keywords
sequence
mirna
target sequence
methods
compositions
Prior art date
Application number
ARP120104034A
Other languages
English (en)
Inventor
Kurek Itzhak
Mgonigle Brian
Zhu Genhai
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of AR088562A1 publication Critical patent/AR088562A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8269—Photosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/01—Hydrolases acting on acid anhydrides (3.6) in phosphorus-containing anhydrides (3.6.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)

Abstract

Se estipulan métodos y composiciones que emplean microRNA (miARN) que, cuando se expresa en una célula vegetal, tiene la capacidad de reducir el nivel de mARN de una secuencia objetivo (es decir, secuencia endógena) sin reducir el nivel de mARN de una o más secuencias estrechamente relacionadas. Mientras que se pueden diseñar miARN con especificidad para una secuencia objetivo particular, la aplicación instantánea demuestra que un miARN puede silenciar específicamente una secuencia objetivo sin silenciar una secuencia estrechamente relacionada que tiene una alta identidad de secuencia con la secuencia objetivo. En ciertas modalidades, una secuencia objetivo endógena puede suprimirse con una construcción de expresión de miARN recombinante sin silenciar un polinucleótido de interés recombinante que tenga una secuencia estrechamente relacionada con la secuencia objetivo. Tales métodos y composiciones emplean construcciones de expresión de miARN recombinante que producen un miARN de 21 nt. Se estipulan, además, células de plantas transgénicas, plantas y semillas que incorporan construcciones de expresión de miARN y construcciones de polinucleótidos recombinantes que comprenden polinucleótidos de interés.
ARP120104034A 2011-10-28 2012-10-26 Metodos y composiciones para silenciar genes usando micro arn artificiales AR088562A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161552700P 2011-10-28 2011-10-28

Publications (1)

Publication Number Publication Date
AR088562A1 true AR088562A1 (es) 2014-06-18

Family

ID=47178344

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120104034A AR088562A1 (es) 2011-10-28 2012-10-26 Metodos y composiciones para silenciar genes usando micro arn artificiales

Country Status (12)

Country Link
US (1) US20130111634A1 (es)
EP (1) EP2771467A1 (es)
CN (1) CN103890179A (es)
AR (1) AR088562A1 (es)
AU (1) AU2012328502A1 (es)
BR (1) BR112014009954A2 (es)
CA (1) CA2850390A1 (es)
IN (1) IN2014DN02042A (es)
MX (1) MX2014004958A (es)
RU (1) RU2014121387A (es)
WO (1) WO2013063487A1 (es)
ZA (1) ZA201401942B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201809406PA (en) * 2016-04-27 2018-11-29 Nexgen Plants Pty Ltd Construct and vector for intragenic plant transformation

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Also Published As

Publication number Publication date
AU2012328502A1 (en) 2014-04-03
WO2013063487A1 (en) 2013-05-02
CN103890179A (zh) 2014-06-25
EP2771467A1 (en) 2014-09-03
MX2014004958A (es) 2014-10-17
IN2014DN02042A (es) 2015-05-15
BR112014009954A2 (pt) 2017-12-05
ZA201401942B (en) 2015-06-24
RU2014121387A (ru) 2015-12-10
US20130111634A1 (en) 2013-05-02
CA2850390A1 (en) 2013-05-02

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